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https://www.readbyqxmd.com/read/27918443/human-intervention-study-to-assess-the-effects-of-supplementation-with-olive-leaf-extract-on-peripheral-blood-mononuclear-cell-gene-expression
#1
Anna Boss, Chi Hsiu-Juei Kao, Pamela M Murray, Gareth Marlow, Matthew P G Barnett, Lynnette R Ferguson
Olive leaf extract (OLE) has been used for many years for its putative health benefits, but, to date, scientific evidence for the basis of these effects has been weak. Although recent literature has described a link between ailments such as cardiovascular disease, diabetes and cancer and a protective effect of polyphenols in the OLE, the mode of action is still unclear. Here, we describe a double-blinded placebo (PBO)-controlled trial, in which gene expression profiles of peripheral blood mononuclear cells from healthy male volunteers (n = 29) were analysed to identify genes that responded to OLE, following an eight-week intervention with 20 mL daily consumption of either OLE or PBO...
December 2, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27889568/fosb-regulates-expression-of-mir-22-during-pma-induced-differentiation-of-k562-cells-to-megakaryocytes
#2
Hafiz M Ahmad, Pamchui Muiwo, Rohini Muthuswami, Alok Bhattacharya
Expression of many miRNAs is altered in different cancers and these changes are thought to play a key role in formation and progression of cancer. In chronic myelogenous leukemia (CML) a number of miRNAs are known to be down regulated as compared to normal cells. In this report we have investigated the mechanism of this down regulation by using PMA induced differentiation of CML cell line K562 to megakaryocytes as an experimental system. On treatment with PMA, expression of many down regulated miRNAs including miR-22 is induced...
November 23, 2016: Biochimie
https://www.readbyqxmd.com/read/27889530/identification-of-proximal-biomarkers-of-pkc-agonism-and-evaluation-of-their-role-in-hiv-reactivation
#3
Sai Vikram Vemula, Jill W Maxwell, Alexey Nefedov, Bang-Lin Wan, Justin Steve, William Newhard, Rosa I Sanchez, David Tellers, Richard J Barnard, Wade Blair, Daria Hazuda, Andrea L Webber, Bonnie J Howell
DESIGN: The HIV latent CD4(+) T cell reservoir is broadly recognized as a barrier to HIV cure. Induction of HIV expression using protein kinase C (PKC) agonists is one approach under investigation for reactivation of latently infected CD4(+) T cells (Beans et al., 2013; Abreu et al., 2014; Jiang et al., 2014; Jiang and Dandekar, 2015). We proposed that an increased understanding of the molecular mechanisms of action of PKC agonists was necessary to inform on biological signaling and pharmacodynamic biomarkers...
November 23, 2016: Antiviral Research
https://www.readbyqxmd.com/read/27878298/trail-overexpression-co-regulated-by-egr1-and-hre-enhances-radiosensitivity-of-hypoxic-a549-cells-depending-on-its-apoptosis-inducing-role
#4
Yan-Ming Yang, Fang Fang, Xin Li, Lei Yu, Zhi-Cheng Wang
Ionizing radiation can upregulate the expression levels of TRAIL and enhance tumor cell apoptosis. While Early growth response 1 (Egr1) gene promoter has radiation inducible characteristics, the expression for exogenous gene controlled by Egr1 promoter could be enhanced by ionizing radiation, but its efficiency is limited by tissue hypoxia. Hypoxia response elements (HREs) are important hypoxic response regulatory sequences and sensitivity enhancers. Therefore, we chose TRAIL as the gene radiotherapy to observe whether it is regulated by Egr1 and HER and its effects on A549 cells and its mechanism...
November 23, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27857060/identification-of-transcription-factors-tfs-and-targets-involved-in-the-cholangiocarcinoma-cca-by-integrated-analysis
#5
L Yang, S Feng, Y Yang
The present study was designed to investigate the upstream transcription factors (TFs) and the signature genes in cholangiocarcinoma (CCA), providing better clues on the regulatory mechanisms and therapeutic applications. Gene expression data sets of CCA were searched in the Gene Expression Omnibus database for integrated analysis. Functional annotation of differently expressed genes (DEGs) was then conducted and the TFs were identified. Moreover, a global transcriptional regulatory network of TFs-targets was constructed...
November 18, 2016: Cancer Gene Therapy
https://www.readbyqxmd.com/read/27852327/ada3-regulates-normal-and-tumor-mammary-epithelial-cell-proliferation-through-c-myc
#6
Nicolas I Griffin, Gayatri Sharma, Xiangshan Zhao, Sameer Mirza, Shashank Srivastava, Bhavana J Dave, Mohammed Aleskandarany, Emad Rakha, Shakur Mohibi, Hamid Band, Vimla Band
BACKGROUND: We have established the critical role of ADA3 as a coactivator of estrogen receptor (ER), as well as its role in cell cycle progression. Furthermore, we showed that ADA3 is predominantly nuclear in mammary epithelium, and in ER+, but is cytoplasmic in ER- breast cancers, the latter correlating with poor survival. However, the role of nuclear ADA3 in human mammary epithelial cells (hMECs), and in ER+ breast cancer cells, as well as the importance of ADA3 expression in relation to patient prognosis and survival in ER+ breast cancer have remained uncharacterized...
November 16, 2016: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/27835650/zhx1-promotes-the-proliferation-migration-and-invasion-of-cholangiocarcinoma-cells
#7
Ryuk-Jun Kwon, Myoung-Eun Han, Ji-Young Kim, Liangwen Liu, Yun-Hak Kim, Jin-Sup Jung, Sae-Ock Oh
Zinc-fingers and homeoboxes 1 (ZHX1) is a transcription repressor that has been associated with the progressions of hepatocellular carcinoma, gastric cancer, and breast cancer. However, the functional roles of ZHX1 in cholangiocarcinoma (CCA) have not been determined. We investigated the expression and roles of ZHX1 during the proliferation, migration, and invasion of CCA cells. In silico analysis and immunohistochemical studies showed amplification and overexpression of ZHX1 in CCA tissues. Furthermore, ZHX1 knockdown using specific siRNAs decreased CCA cell proliferation, migration, and invasion, whereas ZHX1 overexpression promoted all three characteristics...
2016: PloS One
https://www.readbyqxmd.com/read/27820865/egr1-regulates-transcription-downstream-of-mechanical-signals-during-tendon-formation-and-healing
#8
Ludovic Gaut, Nicolas Robert, Antony Delalande, Marie-Ange Bonnin, Chantal Pichon, Delphine Duprez
BACKGROUND: Tendon is a mechanical tissue that transmits forces generated by muscle to bone in order to allow body motion. The molecular pathways that sense mechanical forces during tendon formation, homeostasis and repair are not known. EGR1 is a mechanosensitive transcription factor involved in tendon formation, homeostasis and repair. We hypothesized that EGR1 senses mechanical signals to promote tendon gene expression. METHODOLOGY/PRINCIPAL FINDINGS: Using in vitro and in vivo models, we show that the expression of Egr1 and tendon genes is downregulated in 3D-engineered tendons made of mesenchymal stem cells when tension is released as well as in tendon homeostasis and healing when mechanical signals are reduced...
2016: PloS One
https://www.readbyqxmd.com/read/27809618/identification-of-upstream-transcription-factors-tfs-for-expression-signature-genes-in-breast-cancer
#9
Hongyan Zang, Ning Li, Yuling Pan, Jingguang Hao
Breast cancer is a common malignancy among women with a rising incidence. Our intention was to detect transcription factors (TFs) for deeper understanding of the underlying mechanisms of breast cancer. Integrated analysis of gene expression datasets of breast cancer was performed. Then, functional annotation of differentially expressed genes (DEGs) was conducted, including Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Furthermore, TFs were identified and a global transcriptional regulatory network was constructed...
November 4, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27745706/pka-novel-pkc-isoforms-and-erk-is-mediating-pacap-auto-regulation-via-pac1r-in-human-neuroblastoma-nb-1-cells
#10
Birgitte Georg, Birgitte Falktoft, Jan Fahrenkrug
The neuropeptide PACAP is expressed throughout the central and peripheral nervous system where it modulates diverse physiological functions including neuropeptide gene expression. We here report that in human neuroblastoma NB-1 cells PACAP transiently induces its own expression. Maximal PACAP mRNA expression was found after stimulation with PACAP for 3h. PACAP auto-regulation was found to be mediated by activation of PACAP specific PAC1Rs as PACAP had >100-fold higher efficacy than VIP, and the PAC1R selective agonist Maxadilan potently induced PACAP gene expression...
December 2016: Neuropeptides
https://www.readbyqxmd.com/read/27720869/caffeic-acid-attenuated-acetaminophen-induced-hepatotoxicity-by-inhibiting-erk1-2-mediated-early-growth-response-1-transcriptional-activation
#11
Chun Pang, Liang Shi, Yuchen Sheng, Zhiyong Zheng, Hai Wei, Zhengtao Wang, Lili Ji
Caffeic acid (CA) is a natural compound abundant in fruits, coffee and plants. This study aims to investigate the involved mechanism of the therapeutic detoxification of CA against acetaminophen (APAP)-induced hepatotoxicity. CA (10, 30 mg/kg) was orally given to mice at 1 h after mice were pre-administrated with APAP (300 mg/kg). The therapeutic detoxification of CA against APAP-induced hepatotoxicity was observed by detecting serum aminotransferases, liver malondialdehyde (MDA) amount and liver histological evaluation in vivo...
October 6, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27716817/context-dependent-egr1-expression-in-the-avian-hippocampus
#12
Stephanie L Grella, Mélanie F Guigueno, David J White, David F Sherry, Diano F Marrone
In mammals, episodic memory and spatial cognition involve context-specific recruitment of unique ensembles in the hippocampal formation (HF). Despite their capacity for sophisticated spatial (e.g., for migration) and episodic-like (e.g., for food-caching) memory, the mechanisms underlying contextual representation in birds is not well understood. Here we demonstrate environment-specific Egr1 expression as male brown-headed cowbirds (Molothrus ater) navigate environments for food reward, showing that the avian HF, like its mammalian counterpart, recruits distinct neuronal ensembles to represent different contexts...
2016: PloS One
https://www.readbyqxmd.com/read/27677186/putative-tumor-suppressor-genes-egr1-and-brsk1-are-mutated-in-gastric-and-colorectal-cancers
#13
Eun Ji Choi, Nam Jin Yoo, Min Sung Kim, Chang Hyeok An, Sug Hyung Lee
OBJECTIVE: The transcription factor-encoding EGR1 and the kinase-encoding BRSK1 are considered putative tumor suppressor genes (TSGs). However, EGR1 and BRSK1 mutations that could inactivate their functions are not reported in colorectal (CRC) and gastric (GC) cancers. METHODS: There are mononucleotide repeats in EGR1 and BRSK1, which could be mutated in cancers with defects in mismatch repair, resulting in microsatellite instability (MSI). We analyzed 124 CRCs and 79 GCs for mutations and their intratumoral heterogeneities (ITHs)...
2016: Oncology
https://www.readbyqxmd.com/read/27644246/%C3%AE-aminobutyric-acid-inhibits-the-proliferation-and-increases-oxaliplatin-sensitivity-in-human-colon-cancer-cells
#14
Lihua Song, Aiying Du, Ying Xiong, Jing Jiang, Yao Zhang, Zhaofeng Tian, Hongli Yan
γ-Aminobutyric acid (GABA) is a natural non-protein amino acid, which broadly exists in many plant parts and is widely used as an ingredient in the food industry. In mammals, it is widely distributed in central nervous system and non-neural tissues. In addition to a primary inhibitory neurotransmitter in the central nervous system, endogenous GABA content has been found to be elevated in neoplastic tissues in colon cancer. However, the effect of extraneous GABA on colon cancer has rarely been reported. In this study, we found the inhibitory effects of GABA on the proliferation of colon cancer cells (CCCs)...
September 19, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27642033/a-combination-hepatoma-targeted-therapy-based-on-nanotechnology-phre-egr1-hsv-tk-131-i-antiafpmcab-gcv-mfh
#15
Mei Lin, Junxing Huang, Xingmao Jiang, Jia Zhang, Hong Yu, Jun Ye, Dongsheng Zhang
Combination targeted therapy is a promising cancer therapeutic strategy. Here, using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as magnetic media for MFH (magnetic fluid hyperthermia) and gene transfer vector for gene-therapy, a combined therapy, pHRE-Egr1-HSV-TK/(131)I-antiAFPMcAb-GCV/MFH, for hepatoma is developed. AntiAFPMcAb (Monoclonal antibody AFP) is exploited for targeting. The plasmids pHRE-Egr1-HSV-TK are achieved by incorporation of pEgr1-HSV-TK and pHRE-Egr1-EGFP. Restriction enzyme digestion and PCR confirm the recombinant plasmids pHRE-Egr1-HSV-TK are successfully constructed...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27639037/extracellular-phosphate-induces-the-expression-of-dentin-matrix-protein-1-through-the-fgf-receptor-in-osteoblasts
#16
Jin Nishino, Miwa Yamazaki, Masanobu Kawai, Kanako Tachikawa, Keiko Yamamoto, Kazuaki Miyagawa, Mikihiko Kogo, Keiichi Ozono, Toshimi Michigami
Dentin matrix protein 1 (DMP1) is an extracellular matrix protein involved in phosphate metabolism and biomineralization, and its expression markedly increases during the maturation of osteoblasts into osteocytes. We previously reported that an increased level of inorganic phosphate (Pi) in media up-regulated the expression of Dmp1 in primary osteocytes isolated from mouse bones. In the present study, we found that elevated extracellular Pi strongly induced the expression of Dmp1 in osteoblasts and explored its underlying mechanism of action...
September 17, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27637333/identification-of-novel-nuclear-factor-of-activated-t-cell-nfat-associated-proteins-in-t-cells
#17
Christian H Gabriel, Fridolin Gross, Martin Karl, Heike Stephanowitz, Anna Floriane Hennig, Melanie Weber, Stefanie Gryzik, Ivo Bachmann, Katharina Hecklau, Jürgen Wienands, Johannes Schuchhardt, Hanspeter Herzel, Andreas Radbruch, Eberhard Krause, Ria Baumgrass
Transcription factors of the nuclear factor of activated T cell (NFAT) family are essential for antigen-specific T cell activation and differentiation. Their cooperative DNA binding with other transcription factors, such as AP1 proteins (FOS, JUN, and JUNB), FOXP3, IRFs, and EGR1, dictates the gene regulatory action of NFATs. To identify as yet unknown interaction partners of NFAT, we purified biotin-tagged NFATc1/αA, NFATc1/βC, and NFATc2/C protein complexes and analyzed their components by stable isotope labeling by amino acids in cell culture-based mass spectrometry...
November 11, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27616216/fsh-stimulation-of-anestrous-cats-improves-oocyte-quality-and-development-of-parthenogenetic-embryos
#18
Daniel Veraguas, Paula F Gallegos, Alejandra E Velasquez, Fidel O Castro, Lleretny Rodriguez-Alvarez
In the domestic cat, the efficiency of in vitro embryo production systems is negatively affected during the nonbreeding season. The objective of this research was to evaluate the effect of FSH stimulation in anestrous cats, on quality of cumulus-oocyte complexes (COCs) and in vitro developmental competence after parthenogenetic activation. To accomplish this purpose, anestrous cats were grouped into: (1) FSH treated (serial doses of 5 mg of porcine FSH each, every 24 hours, for 4 days) and (2) untreated control...
August 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27587583/genome-wide-co-localization-of-active-egfr-and-downstream-erk-pathway-kinases-mirrors-mitogen-inducible-rna-polymerase-2-genomic-occupancy
#19
M Mikula, M Skrzypczak, K Goryca, K Paczkowska, J K Ledwon, M Statkiewicz, M Kulecka, M Grzelak, M Dabrowska, U Kuklinska, J Karczmarski, I Rumienczyk, K Jastrzebski, M Miaczynska, K Ginalski, K Bomsztyk, J Ostrowski
Genome-wide mechanisms that coordinate expression of subsets of functionally related genes are largely unknown. Recent studies show that receptor tyrosine kinases and components of signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), once thought to act predominantly in the vicinity of plasma membrane and in the cytoplasm, can be recruited to chromatin encompassing transcribed genes. Genome-wide distribution of these transducers and their relationship to transcribing RNA polymerase II (Pol2) could provide new insights about co-regulation of functionally related gene subsets...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27582276/epigenetic-regulation-of-transcription-factor-promoter-regions-by-low-dose-genistein-through-mitogen-activated-protein-kinase-and-mitogen-and-stress-activated-kinase-1-nongenomic-signaling
#20
Linda Yu, Kyle Ham, Xiaohua Gao, Lysandra Castro, Yitang Yan, Grace E Kissling, Charles J Tucker, Norris Flagler, Ray Dong, Trevor K Archer, Darlene Dixon
BACKGROUND: The phytoestrogen, genistein at low doses nongenomically activates mitogen-activated protein kinase p44/42 (MAPKp44/42) via estrogen receptor alpha (ERα) leading to proliferation of human uterine leiomyoma cells. In this study, we evaluated if MAPKp44/42 could activate downstream effectors such as mitogen- and stress-activated protein kinase 1 (MSK1), which could then epigenetically modify histone H3 by phosphorylation following a low dose (1 μg/ml) of genistein. RESULTS: Using hormone-responsive immortalized human uterine leiomyoma (ht-UtLM) cells, we found that genistein activated MAPKp44/42 and MSK1, and also increased phosphorylation of histone H3 at serine10 (H3S10ph) in ht-UtLM cells...
2016: Cell Communication and Signaling: CCS
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