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https://www.readbyqxmd.com/read/29681936/metformin-regulating-mir-34a-pathway-to-inhibit-egr1-in-rat-mesangial-cells-cultured-with-high-glucose
#1
Can Wu, Ningning Qin, Huiwen Ren, Min Yang, Shuang Liu, Qiuyue Wang
Background: Activating AMPK α negatively regulates Egr1 to inhibit inflammatory cytokines in high glucose. miR-34a inhibition increases phosphorylated AMPK α through mediating SIRT1 to suppress the development of fatty liver. Aim of the Study: To clarify the function of Egr1 on the inflammation and fibrosis in high glucose-cultured MCs, as well as to explore the effects of metformin on miR-34a pathway and Egr1 expression. Methods: We transfected MCs with miR-34a inhibitor...
2018: International Journal of Endocrinology
https://www.readbyqxmd.com/read/29679052/differential-contribution-of-cis-and-trans-gene-transcription-regulatory-mechanisms-in-amygdala-and-prefrontal-cortex-and-modulation-by-social-stress
#2
Eli Reuveni, Dmitry Getselter, Oded Oron, Evan Elliott
While both individual transcription factors and cis-acting sites have been studied in relation to psychiatric disorders, there is little knowledge of the relative contribution of trans-acting and cis-acting factors to gene transcription in the brain. Using an RNA-seq approach in mice bred from two evolutionary-distinct mice strains, we determined the contribution of cis and trans factors to gene expression in the prefrontal cortex and amygdala, two regions of the brain relevant to the stress response, and the contribution of cis and trans factors in the prefrontal cortex after Chronic Social Defeat (CSD) in mice...
April 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29669292/parn-and-toe1-constitute-a-3-end-maturation-module-for-nuclear-non-coding-rnas
#3
Ahyeon Son, Jong-Eun Park, V Narry Kim
Poly(A)-specific ribonuclease (PARN) and target of EGR1 protein 1 (TOE1) are nuclear granule-associated deadenylases, whose mutations are linked to multiple human diseases. Here, we applied mTAIL-seq and RNA sequencing (RNA-seq) to systematically identify the substrates of PARN and TOE1 and elucidate their molecular functions. We found that PARN and TOE1 do not modulate the length of mRNA poly(A) tails. Rather, they promote the maturation of nuclear small non-coding RNAs (ncRNAs). PARN and TOE1 act redundantly on some ncRNAs, most prominently small Cajal body-specific RNAs (scaRNAs)...
April 17, 2018: Cell Reports
https://www.readbyqxmd.com/read/29644781/inpp4b-overexpression-and-c-kit-downregulation-in-human-achalasia
#4
E Bonora, F Bianco, A Stanzani, F Giancola, A Astolfi, V Indio, C Evangelisti, A M Martelli, E Boschetti, M Lugaresi, A Ioannou, F Torresan, V Stanghellini, P Clavenzani, M Seri, A Moonen, K Van Beek, M Wouters, G E Boeckxstaens, G Zaninotto, S Mattioli, R De Giorgio
BACKGROUND: Achalasia is a rare motility disorder characterized by myenteric neuron and interstitial cells of Cajal (ICC) abnormalities leading to deranged/absent peristalsis and lack of relaxation of the lower esophageal sphincter. The mechanisms contributing to neuronal and ICC changes in achalasia are only partially understood. Our goal was to identify novel molecular features occurring in patients with primary achalasia. METHODS: Esophageal full-thickness biopsies from 42 (22 females; age range: 16-82 years) clinically, radiologically, and manometrically characterized patients with primary achalasia were examined and compared to those obtained from 10 subjects (controls) undergoing surgery for uncomplicated esophageal cancer (or upper stomach disorders)...
April 11, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29614078/a-bi-stable-feedback-loop-between-gdnf-egr1-and-er%C3%AE-contribute-to-endocrine-resistant-breast-cancer
#5
Sachi Horibata, Edward J Rice, Hui Zheng, Chinatsu Mukai, Tinyi Chu, Brooke A Marks, Scott A Coonrod, Charles G Danko
Discovering regulatory interactions between genes that specify the behavioral properties of cells remains an important challenge. We used the dynamics of transcriptional changes resolved by PRO-seq to identify a regulatory network responsible for endocrine resistance in breast cancer. We show that GDNF leads to endocrine resistance by switching the active state in a bi-stable feedback loop between GDNF, EGR1, and the master transcription factor ERα. GDNF stimulates MAP kinase, activating the transcription factors SRF and AP-1...
2018: PloS One
https://www.readbyqxmd.com/read/29602637/importin-11-overexpression-promotes-the-migration-invasion-and-progression-of-bladder-cancer-associated-with-the-deregulation-of-cdkn1a-and-thbs1
#6
Junjie Zhao, Lei Shi, Shuxiong Zeng, Chong Ma, Weidong Xu, Zhensheng Zhang, Qingzuo Liu, Peng Zhang, Yinghao Sun, Chuanliang Xu
OBJECTIVES: We recently determined that a novel oncogene, IPO11 from 5q12, participates in bladder cancer (BCa) progression. However, the biological function of IPO11 and the molecular mechanisms through which it contributes to BCa progression remain unclear. The aim of this study was to investigate the role of IPO11 in BCa aggressiveness and elucidate the molecular mechanisms underlying its effects in BCa. MATERIALS AND METHODS: The mRNA expression levels of IPO11 in BIU-87, RT4, UMUC3, EJ, 5637, T24, J82, and HT-1376 cell lines were determined using quantitative real-time polymerase chain reaction...
March 27, 2018: Urologic Oncology
https://www.readbyqxmd.com/read/29601929/6-ohbde-47-induces-transcriptomic-alterations-of-cyp1a1-xrcc2-hspa1a-egr1-genes-and-trigger-apoptosis-in-hepg2-cells
#7
Quaiser Saquib, Maqsood A Siddiqui, Javed Ahmad, Sabiha M Ansari, Hend A Al-Wathnani, Christopher Rensing
In this study, HepG2 cells were exposed to 6-hydroxy- 2,2',4,4'-tetrabromodiphenyl ether (6-OHBDE-47) for 3 and 6 days for monitoring cytotoxic effects and alterations in its transcriptomic profile. MTT assay showed that cells exposed to 6-OHBDE-47 (50 nM) exhibited 48.5% and 53.7% decline in cell survival after 3 and 6 days. Neutral red uptake (NRU) assay also demonstrated 47.1% and 56% reduction in cell survival at 50 nM, indicating lysosomal toxicity. The flow cytometric data confirmed an increase in intracellular reactive oxygen species (ROS) and mitochondrial dysfunction (ΔYm)...
March 27, 2018: Toxicology
https://www.readbyqxmd.com/read/29590119/nf90-ilf3-is-a-transcription-factor-that-promotes-proliferation-over-differentiation-by-hierarchical-regulation-in-k562-erythroleukemia-cells
#8
Ting-Hsuan Wu, Lingfang Shi, Jessika Adrian, Minyi Shi, Ramesh V Nair, Michael P Snyder, Peter N Kao
NF90 and splice variant NF110 are DNA- and RNA-binding proteins encoded by the Interleukin enhancer-binding factor 3 (ILF3) gene that have been established to regulate RNA splicing, stabilization and export. The roles of NF90 and NF110 in regulating transcription as chromatin-interacting proteins have not been comprehensively characterized. Here, chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) identified 9,081 genomic sites specifically occupied by NF90/NF110 in K562 cells. One third of NF90/NF110 peaks occurred at promoters of annotated genes...
2018: PloS One
https://www.readbyqxmd.com/read/29568765/the-potential-protective-effect-of-curcumin-on-amyloid-%C3%AE-42-induced-cytotoxicity-in-ht-22-cells
#9
Lu Zhang, Yu Fang, Xuan Cheng, Yajun Lian, Zhaoshu Zeng, Chuanjie Wu, Hongcan Zhu, Hongliang Xu
Background: We aimed to investigate the effect and mechanism of curcumin (CUR) in Alzheimer's disease (AD). Methods: Mouse hippocampal neuronal cell line HT-22 was treated with A β 1-42 and/or CUR, and then cell viability was evaluated by cell counting kit 8, Beclin-l level was detected using western blotting, and the formation of autophagosomes was observed by transmission electron microscopy (TEM). Furthermore, transcriptome sequencing and analysis were performed in cells with A β 1-42 alone or A β 1-42 + CUR...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29567506/phylogeny-of-the-subfamily-stelliferinae-suggests-speciation-in-ophioscion-gill-1863-sciaenidae-perciformes-in-the-western-south-atlantic
#10
Tárcia Fernanda da Silva, Horacio Schneider, Iracilda Sampaio, Arturo Angulo, Marcelo Fulgêncio Guedes Brito, Alexandre Clistenes de Alcântara Santos, Jonas de Andrade Santos, Alfredo Carvalho-Filho, Simoni Santos
Phylogenies based on morphological and molecular data confirm the monophyly of the subfamily Stelliferinae; however, there is no consensus on the intergeneric and interspecific relationships in the group. Previous studies suggested the non-monophyly of Ophioscion and Stellifer, and possible cryptic species in Ophioscion punctatissimus. Therefore, we used mitochondrial (16S rDNA and COI) and nuclear (Rhodopsin, EGR1, and RAG1) regions to examine phylogenetic relationships among species of this subfamily. Our results confirmed the monophyly of Stelliferinae and supports the close relationship among Bardiella, Corvula and Odontoscion, which form a sister group to Stellifer and Ophioscion...
March 19, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29546053/-positive-regulation-of-rna-metabolic-process-ontology-group-highly-regulated-in-porcine-oocytes-matured-in-vitro-a-microarray-approach
#11
Piotr Celichowski, Mariusz J Nawrocki, Marta Dyszkiewicz-Konwińska, Maurycy Jankowski, Joanna Budna, Artur Bryja, Wiesława Kranc, Sylwia Borys, Sandra Knap, Sylwia Ciesiółka, Michal Jeseta, Karolina Piasecka-Stryczyńska, Ronza Khozmi, Dorota Bukowska, Paweł Antosik, Klaus P Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29529029/m%C3%A3-ller-glia-derived-prss56-is-required-to-sustain-ocular-axial-growth-and-prevent-refractive-error
#12
Seyyedhassan Paylakhi, Cassandre Labelle-Dumais, Nicholas G Tolman, Michael A Sellarole, Yusef Seymens, Joseph Saunders, Hesham Lakosha, Wilhelmine N deVries, Andrew C Orr, Piotr Topilko, Simon Wm John, K Saidas Nair
A mismatch between optical power and ocular axial length results in refractive errors. Uncorrected refractive errors constitute the most common cause of vision loss and second leading cause of blindness worldwide. Although the retina is known to play a critical role in regulating ocular growth and refractive development, the precise factors and mechanisms involved are poorly defined. We have previously identified a role for the secreted serine protease PRSS56 in ocular size determination and PRSS56 variants have been implicated in the etiology of both hyperopia and myopia, highlighting its importance in refractive development...
March 12, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29520222/immediate-early-genes-anchor-a-biological-pathway-of-proteins-required-for-memory-formation-long-term-depression-and-risk-for-schizophrenia
#13
Ketan K Marballi, Amelia L Gallitano
While the causes of myriad medical and infectious illnesses have been identified, the etiologies of neuropsychiatric illnesses remain elusive. This is due to two major obstacles. First, the risk for neuropsychiatric disorders, such as schizophrenia, is determined by both genetic and environmental factors. Second, numerous genes influence susceptibility for these illnesses. Genome-wide association studies have identified at least 108 genomic loci for schizophrenia, and more are expected to be published shortly...
2018: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/29511684/oncogenic-n-ras-stimulates-srf-mediated-transactivation-via-h3-acetylation-at-lysine-9
#14
Sun-Ju Yi, Seong Yun Hwang, Myung-Ju Oh, Yang-Hoon Kim, Hojin Ryu, Sung-Keun Rhee, Byung H Jhun, Kyunghwan Kim
Signal transduction pathways regulate the gene expression by altering chromatin dynamics in response to mitogens. Ras proteins are key regulators linking extracellular stimuli to a diverse range of biological responses associated with gene regulation. In mammals, the three ras genes encode four Ras protein isoforms: H-Ras, K-Ras4A, K-Ras4B, and N-Ras. Although emerging evidence suggests that Ras isoforms differentially regulate gene expressions and are functionally nonredundant, the mechanisms underlying Ras specificity and Ras signaling effects on gene expression remain unclear...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29511351/hpse-enhancer-rna-promotes-cancer-progression-through-driving-chromatin-looping-and-regulating-hnrnpu-p300-egr1-hpse-axis
#15
Wanju Jiao, Yajun Chen, Huajie Song, Dan Li, Hong Mei, Feng Yang, Erhu Fang, Xiaojing Wang, Kai Huang, Liduan Zheng, Qiangsong Tong
Recent studies reveal the emerging functions of enhancer RNAs (eRNAs) in gene expression. However, the roles of eRNAs in regulating the expression of heparanase (HPSE), an established endo-β-D-glucuronidase essential for cancer invasion and metastasis, still remain elusive. Herein, through comprehensive analysis of publically available FANTOM5 expression atlas and chromatin interaction dataset, we identified a super enhancer and its derived eRNA facilitating the HPSE expression (HPSE eRNA) in cancers. Gain-of-function and loss-of-function experiments indicated that HPSE eRNA facilitated the in vitro and in vivo tumorigenesis and aggressiveness of cancer cells...
March 7, 2018: Oncogene
https://www.readbyqxmd.com/read/29511024/genome-wide-strategies-identify-downstream-target-genes-of-connective-tissue-associated-transcription-factors
#16
Mickael Orgeur, Marvin Martens, Georgeta Leonte, Sonya Nassari, Marie-Ange Bonnin, Stefan T Börno, Bernd Timmermann, Jochen Hecht, Delphine Duprez, Sigmar Stricker
Connective tissues support organs and play crucial roles in development, homeostasis and fibrosis, yet our understanding of their formation is still limited. To gain insight into the molecular mechanisms of connective tissue specification, we selected five zinc finger transcription factors - OSR1, OSR2, EGR1, KLF2 and KLF4 - based on their expression patterns and/or known involvement in connective tissue subtype differentiation. RNA-seq and ChIP-seq profiling revealed a set of common genes regulated by all five transcription factors, which we propose as connective tissue core expression set...
March 6, 2018: Development
https://www.readbyqxmd.com/read/29494759/transcriptional-co-repressor-sin3a-silencing-rescues-decline-in-memory-consolidation-during-scopolamine-induced-amnesia
#17
Sweta Srivas, Mahendra K Thakur
Epigenetic modifications through methylation of DNA and acetylation of histones modulate neuronal gene expression and regulate long-term memory. Earlier we demonstrated that scopolamine-induced decrease in memory consolidation is correlated with enhanced expression of hippocampal DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) in mice. DNMT1 and HDAC2 act together by recruiting a co-repressor complex and deacetylating the chromatin. The catalytic activity of HDACs is mainly dependent on its incorporation into multiprotein co-repressor complexes, among which SIN3A-HDAC2 co-repressor is widely studied to regulate synaptic plasticity...
March 1, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29487594/beta-defensin-2-and-3-promote-bacterial-clearance-of-pseudomonas-aeruginosa-by-inhibiting-macrophage-autophagy-through-downregulation-of-early-growth-response-gene-1-and-c-fos
#18
Yongjian Wu, Dandan Li, Yi Wang, Xi Liu, Yuanqing Zhang, Wenting Qu, Kang Chen, Ngiambudulu M Francisco, Lianqiang Feng, Xi Huang, Minhao Wu
Beta-defensins 2 and 3 (BD2 and BD3) are inducible peptides present at the sites of infection, and they are well characterized for their antimicrobial activities and immune-regulatory functions. However, no study has thoroughly investigated their immunomodulatory effects on macrophage-mediated immune responses against Pseudomonas aeruginosa (PA). Here, we use THP-1 and RAW264.7 cell lines and demonstrate that BD2 and BD3 suppressed macrophage autophagy but enhanced the engulfment of PA and Zymosan bioparticles as well as the formation of phagolysosomes, using immunofluorescence staining and confocal microscopy...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29487567/regulatory-architecture-of-the-l%C3%AE-t2-gonadotrope-cell-underlying-the-response-to-gonadotropin-releasing-hormone
#19
Frederique Ruf-Zamojski, Miguel Fribourg, Yongchao Ge, Venugopalan Nair, Hanna Pincas, Elena Zaslavsky, German Nudelman, Stephanie J Tuminello, Hideo Watanabe, Judith L Turgeon, Stuart C Sealfon
The LβT2 mouse pituitary cell line has many characteristics of a mature gonadotrope and is a widely used model system for studying the developmental processes and the response to gonadotropin-releasing hormone (GnRH). The global epigenetic landscape, which contributes to cell-specific gene regulatory mechanisms, and the single-cell transcriptome response variation of LβT2 cells have not been previously investigated. Here, we integrate the transcriptome and genome-wide chromatin accessibility state of LβT2 cells during GnRH stimulation...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29478859/fmri-reveals-a-novel-region-for-evaluating-acoustic-information-for-mate-choice-in-a-female-songbird
#20
Lisbeth Van Ruijssevelt, Yining Chen, Kaya von Eugen, Julie Hamaide, Geert De Groof, Marleen Verhoye, Onur Güntürkün, Sarah C Woolley, Annemie Van der Linden
Selection of sexual partners is among the most critical decisions that individuals make and is therefore strongly shaped by evolution. In social species, where communication signals can convey substantial information about the identity, state, or quality of the signaler, accurate interpretation of communication signals for mate choice is crucial. Despite the importance of social information processing, to date, relatively little is known about the neurobiological mechanisms that contribute to sexual decision making and preferences...
February 21, 2018: Current Biology: CB
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