keyword
MENU ▼
Read by QxMD icon Read
search

egr1

keyword
https://www.readbyqxmd.com/read/29241165/sc1-promotes-mir124-3p-expression-to-maintain-the-self-renewal-of-mouse-embryonic-stem-cells-by-inhibiting-the-mek-erk-pathway
#1
Qing Wei, Hongliang Liu, Zhiying Ai, Yongyan Wu, Yingxiang Liu, Zhaopeng Shi, Xuexue Ren, Zekun Guo
BACKGROUND/AIMS: Self-renewal is one of the most important features of embryonic stem (ES) cells. SC1 is a small molecule modulator that effectively maintains the self-renewal of mouse ES cells in the absence of leukemia inhibitory factor (LIF), serum and feeder cells. However, the mechanism by which SC1 maintains the undifferentiated state of mouse ES cells remains unclear. METHODS: In this study, microarray and small RNA deep-sequencing experiments were performed on mouse ES cells treated with or without SC1 to identify the key genes and microRNAs that contributed to self-renewal...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29235250/discovery-and-mechanistic-elucidation-of-a-class-of-pdi-inhibitors-for-the-treatment-of-glioblastoma
#2
Anahita Kyani, Shuzo Tamura, Suhui Yang, Andrea Shergalis, Soma Somanta, Yuting Kuang, Mats Ljungman, Nouri Neamati
Protein disulfide isomerase (PDI) is overexpressed in glioblastoma, the most aggressive form of brain cancer, and folds nascent proteins responsible for the progression and spread of the disease. Herein, we describe a novel, nanomolar PDI inhibitor, pyrimidotriazinedione 35G8, that is toxic in a panel of human glioblastoma cell lines. We performed a medium throughput 20,000-compound screen of a diverse subset of 1,000,000 compounds to identify cytotoxic small molecules. Cytotoxic compounds were screened for PDI inhibition, and, from the screen, 35G8 emerged as the most cytotoxic inhibitor of PDI...
December 12, 2017: ChemMedChem
https://www.readbyqxmd.com/read/29228577/egr1-regulates-cellular-metabolism-and-survival-in-endocrine-resistant-breast-cancer
#3
Ayesha N Shajahan-Haq, Simina M Boca, Lu Jin, Krithika Bhuvaneshwar, Yuriy Gusev, Amrita K Cheema, Diane D Demas, Kristopher S Raghavan, Ryan Michalek, Subha Madhavan, Robert Clarke
About 70% of all breast cancers are estrogen receptor alpha positive (ER+; ESR1). Many are treated with antiestrogens. Unfortunately, de novo and acquired resistance to antiestrogens is common but the underlying mechanisms remain unclear. Since growth of cancer cells is dependent on adequate energy and metabolites, the metabolomic profile of endocrine resistant breast cancers likely contains features that are deterministic of cell fate. Thus, we integrated data from metabolomic and transcriptomic analyses of ER+ MCF7-derived breast cancer cells that are antiestrogen sensitive (LCC1) or resistant (LCC9) that resulted in a gene-metabolite network associated with EGR1 (early growth response 1)...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212169/ccctc-binding-factor-inhibits-breast-cancer-cell-proliferation-and-metastasis-via-inactivation-of-the-nuclear-factor-kappab-pathway
#4
Jie Wu, Peng-Chang Li, Jun-Yi Pang, Guo-You Liu, Xue-Min Xie, Jia-Yao Li, Yi-Cong Yin, Jian-Hua Han, Xiu-Zhi Guo, Ling Qiu
CCCTC-binding factor (CTCF) is an important epigenetic regulator implicated in multiple cellular processes, including growth, proliferation, differentiation, and apoptosis. Although CTCF deletion or mutation has been associated with human breast cancer, the role of CTCF in breast cancer is questionable. We investigated the biological functions of CTCF in breast cancer and the underlying mechanism. The results showed that CTCF expression in human breast cancer cells and tissues was significantly lower than that in normal breast cells and tissues...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29198014/inhibition-of-complement-drives-increase-in-early-growth-response-proteins-and-neuroprotection-mediated-by-salidroside-after-cerebral-ischemia
#5
Wenfang Lai, XiuLi Xie, Xiaoqin Zhang, Yingzheng Wang, Kedan Chu, John Brown, Lidian Chen, Guizhu Hong
Salidroside is neuroprotective across a wide therapeutic time-window after cerebral ischemia-reperfusion injury (IRI). Here, we investigated the role of complement in mediating effects of salidroside after cerebral IRI in rats. Rats were administrated with vehicle or salidroside 50 mg/kg, given daily for either 24 or 48 h, after middle cerebral artery occlusion (MCAO) for 2 h and reperfusion for 1 h. Levels of proteins in ischemic brain were measured by immunofluorescence and western blotting. We observed early increases in the deposition of immunoglobulin M, mannose-binding lectin 2, and annexin IV on cerebral endothelial cells, induction of the complement components C3 and C3a, by 24 h after IRI, and a later significant increase in the complement component C1q by 48 h...
December 2, 2017: Inflammation
https://www.readbyqxmd.com/read/29197744/transcriptional-landscape-of-a-retc634y-mutated-ipsc-and-its-crispr-corrected-isogenic-control-reveals-the-putative-role-of-egr1-transcriptional-program-in-the-development-of-multiple-endocrine-neoplasia-type-2a-associated-cancers
#6
Julien Hadoux, Christophe Desterke, Olivier Féraud, Mathieu Guibert, Roberta Francesca De Rose, Paule Opolon, Dominique Divers, Emilie Gobbo, Frank Griscelli, Martin Schlumberger, Annelise Bennaceur-Griscelli, Ali G Turhan
MEN2A is a hereditary cancer-predisposing syndrome that affects patients with germline RET mutations. The effects of this oncogenic tyrosine kinase in the context of primitive stem cells are not known. In order to study these events, we generated a MEN2A induced Pluripotent Stem Cell (iPSC) line from a patient with RET mutation and an isogenic counterpart by CRISPR-Cas9 correction of the mutation. Whole exome sequencing of iPSC before and after CRISPR-Cas9 genome edition revealed no major exonic off target effect of the CRISPR correction...
November 23, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29197151/transcriptional-regulation-of-cacybp-sip-gene-and-the-influence-of-increased-cacybp-sip-level-on-gene-expression-pattern-in-colorectal-cancer-hct116-cells
#7
Beata Kądziołka, Konrad J Dębski, Paweł Bieganowski, Wiesława Leśniak, Anna Filipek
The CacyBP/SIP protein is expressed at a particularly high level in brain, spleen, and various tumors. In this work, we have studied transcriptional regulation of the CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression in colorectal cancer HCT116 cells. We have shown that E2F1, EGR1, and CREB transcription factors bind to the CacyBP/SIP gene promoter and stimulate transcription of CacyBP/SIP gene. The role of CREB was further confirmed by the observation that forskolin, a strong activator of CREB phosphorylation/activity, increased CacyBP/SIP gene promoter activity...
December 1, 2017: IUBMB Life
https://www.readbyqxmd.com/read/29196218/stress-induced-hippocampus-npas4-mrna-expression-relates-to-specific-psychophysiological-patterns-in-stress-response
#8
Jean-Baptiste Drouet, André Peinnequin, Patrice Faure, Josiane Denis, Nadine Fidier, Renaud Maury, Alain Buguet, Raymond Cespuglio, Frédéric Canini
Neuronal Per-Arnt-Sim (PAS) domain protein4 (Npas4) is a key protein that intervenes in GABA synapse scaling and neurotrophicity enhancing. Since GABA and neurotrophicity are implicated in stress response and Npas4-deficient rodents exhibit behavioral alterations, an investigation was designed in rats to verify whether stress-induced spontaneous hippocampus Npas4 mRNA expression would be associated with specific patterns of stress response. The rats were exposed to one of three stressor levels: no stress (CTL, n=15), exposure to a footshock apparatus (Sham, S, n=40) and footshock (F, n=80)...
November 28, 2017: Brain Research
https://www.readbyqxmd.com/read/29194616/comparative-analysis-of-single-cell-rna-sequencing-data-from-mouse-spermatogonial-and-mesenchymal-stem-cells-to-identify-differentially-expressed-genes-and-transcriptional-regulators-of-germline-cells
#9
Sajjad Sisakhtnezhad, Parvin Heshmati
Identifying effective internal factors for regulating germline commitment during development and for maintaining spermatogonial stem cells (SSCs) self-renewal is important to understand the molecular basis of spermatogenesis process, and to develop new protocols for the production of the germline cells from other cell sources. Therefore, this study was designed to investigate single-cell RNA-sequencing data for identification of differentially expressed genes (DEGs) in 12 mouse-derived single SSCs (mSSCs) in compare with 16 mouse-derived single mesenchymal stem cells...
December 1, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29171282/-3r-5-6-7-trihydroxy-3-isopropyl-3-methylisochroman-1-one-alleviates-lipoteichoic-acid-induced-photoreceptor-cell-damage
#10
En-Hui Yi, Feng Xu, Peng Li
Exposure to oxidative stress will lead to the progression of retinal degenerative diseases, and unfortunately the exact mechanisms have not been fully understood. In this study, the protective effects of (3R)-5,6,7-trihydroxy-3-isopropyl-3-methylisochroman-1-one (TIM) against the lipoteichoic acid (LTA)-induced cell damage in mouse photoreceptor-derived 661W cells were investigated. 661W cells were pre-treated with TIM at different concentrations (0.1-2.5 μM) before exposure to LTA. The oxidative stress and inflammatory response were detected in 661W cells...
November 24, 2017: Cutaneous and Ocular Toxicology
https://www.readbyqxmd.com/read/29170465/egr1-deficiency-induces-browning-of-inguinal-subcutaneous-white-adipose-tissue-in-mice
#11
Cécile Milet, Marianne Bléher, Kassandra Allbright, Mickael Orgeur, Fanny Coulpier, Delphine Duprez, Emmanuelle Havis
Beige adipocyte differentiation within white adipose tissue, referred to as browning, is seen as a possible mechanism for increasing energy expenditure. The molecular regulation underlying the thermogenic browning process has not been entirely elucidated. Here, we identify the zinc finger transcription factor EGR1 as a negative regulator of the beige fat program. Loss of Egr1 in mice promotes browning in the absence of external stimulation and leads to an increase of Ucp1 expression, which encodes the key thermogenic mitochondrial uncoupling protein-1...
November 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29169284/vegfc-vegfr3-signaling-regulates-mouse-spermatogonial-cell-proliferation-via-the-activation-of-akt-mapk-and-cyclin-d1-pathway-and-mediates-the-apoptosis-by-affecting-caspase-3-9-and-bcl-2
#12
Liangyu Zhao, Zijue Zhu, Chencheng Yao, Yuhua Huang, Erlei Zhi, Huixing Chen, Ruhui Tian, Peng Li, Qingqing Yuan, Yunjing Xue, Zhong Wan, Chao Yang, Yuehua Gong, Zuping He, Zheng Li
We have previously shown that the transcript levels of Vegfc and its receptor Vegfr3 were high in spermatogonia and extremely low in spermatocytes and spermatids. However, it remains unknown about the functions and the mechanisms of VEGFC/VEGFR3 signaling in regulating the fate determinations of spermatogonia. To this end, here we explored the role and signaling pathways of VEGFC/VEGFR3 by using a cell line derived from immortalized mouse spermatogonia retaining markers of mitotic germ cells, namely GC-1 cells...
November 23, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29168882/fibroblast-growth-factor-2-regulates-activity-and-gene-expression-of-human-postmitotic-excitatory-neurons
#13
Shweta Gupta, Tanya M-Redmond, Fan Meng, Andrew Tidball, Huda Akil, Stanley Watson, Jack M Parent, Michael Uhler
Many neuropsychiatric disorders are thought to result from subtle changes in neural circuit formation. We used human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to model mature, postmitotic excitatory neurons and examine effects of FGF2. FGF2 gene expression is known to be altered in brain regions of major depressive disorder (MDD) patients and FGF2 has anti-depressive effects in animal models of depression. We generated stable inducible neurons (siNeurons) conditionally expressing human neurogenin 2 (NEUROG2) to generate a homogenous population of post-mitotic excitatory neurons and study the functional as well as the transcriptional effects of FGF2...
November 23, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29149041/the-hmga1-pseudogene-7-induces-mir-483-and-mir-675-upregulation-by-activating-egr1-through-a-cerna-mechanism
#14
Marco De Martino, Giuseppe Palma, Amalia Azzariti, Claudio Arra, Alfredo Fusco, Francesco Esposito
Several studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudogenes have a critical role in cancer progression, acting as micro RNA (miRNA) sponges for HMGA1 and other cancer-related genes. HMGA1 pseudogenes were found overexpressed in several human carcinomas, and their expression levels positively correlate with an advanced cancer stage and a poor prognosis...
November 17, 2017: Genes
https://www.readbyqxmd.com/read/29146189/oxidized-low-density-lipoprotein-promotes-osteoclast-differentiation-from-cd68-positive-mononuclear-cells-by-regulating-hmgb1-release
#15
Chunfeng Hou, Luan Luan, Chunfeng Ren
Patients with ankylosing spondylitis (AS) have an increased risk for cardiovascular mortality. The circulating ox-LDL/LDL ratio is associated with subclinical atherosclerosis in patients with systemic lupus erythematosus. In this study, we found that the ox-LDL/LDL ratio was increased in AS patients. The levels of serum RANKL and HMGB1 were also elevated in AS patients, and the number of CD68(+)/RANK(+) cells was increased in peripheral blood from AS patients. 0.03% ox-LDL in LDL, similar to the ox-LDL/LDL ratio in peripheral blood from AS patients, promoted cytoplasmic translocation and release of HMGB1 as well as RANK expression...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29138967/mice-lacking-egr1-have-impaired-clock-gene-bmal1-oscillation-locomotor-activity-and-body-temperature
#16
Casper Schwartz Riedel, Birgitte Georg, Henrik L Jørgensen, Jens Hannibal, Jan Fahrenkrug
Early growth response transcription factor 1 (EGR1) is expressed in the suprachiasmatic nucleus (SCN) after light stimulation. We used EGR1-deficient mice to address the role of EGR1 in the clock function and light-induced resetting of the clock. The diurnal rhythms of expression of the clock genes BMAL1 and PER1 in the SCN were evaluated by semi-quantitative in situ hybridization. We found no difference in the expression of PER1 mRNA between wildtype and EGR1-deficient mice; however, the daily rhythm of BMAL1 mRNA was completely abolished in the EGR1-deficient mice...
November 14, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29129208/augmented-gene-expression-triggered-by-na-k-atpase-inhibition-role-of-ca-2-i-mediated-and-independent-excitation-transcription-coupling
#17
Larisa V Smolyaninova, Svetlana V Koltsova, Svetlana V Sidorenko, Sergei N Orlov
In rat vascular smooth muscle cells (RVSMC), 3-h Na(+),K(+)-ATPase inhibition by ouabain or in K(+)-free medium resulted in the inversion of the [Na(+)]i/[K(+)]i ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca(2+) influx by 2-fold that was abolished by L-type voltage-gated Ca(2+) channel blocker nicardipine, but it was resistant to Na(+)/Ca(2+) exchanger inhibitor KB-R7943. To study the role of Ca(2+)-mediated signaling in the expression of Na(+)i/K(+)i-sensitive genes we used intracellular Ca(2+) chelator BAPTA and incubated RVSMC in Ca(2+)-free medium...
December 2017: Cell Calcium
https://www.readbyqxmd.com/read/29122838/soluble-cd80-protein-delays-tumor-growth-and-promotes-tumor-infiltrating-lymphocytes
#18
Lucas A Horn, Tiha M Long, Ryan Atkinson, Virginia Clements, Suzanne Ostrand-Rosenberg
Tumor cells employ various immune suppressive strategies to overcome antitumor immunity. One such method is tumor expression of programmed death ligand-1 (PD-L1), which triggers apoptotic death or anergy upon binding programmed death-1 (PD-1) on T cells. Our previous in vitro cellular studies with human and mouse PD-L1+ tumor cells demonstrated that a soluble form of the costimulatory molecule CD80 prevented PD-L1-mediated immune suppression and restored T-cell activation by binding PD-L1 and blocking interaction with PD-1...
November 9, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/29105401/bioinformatic-analysis-identifies-three-potentially-key-differentially-expressed-genes-in-peripheral-blood-mononuclear-cells-of-patients-with-takayasu-s-arteritis
#19
Renping Huang, Yang He, Bei Sun, Bing Liu
OBJECTIVES: This study aimed to identify several potentially key genes associated with the pathogenesis of Takayasu's arteritis (TA). This identification may lead to a deeper mechanistic understanding of TA etiology and pave the way for potential therapeutic approaches. MATERIALS AND METHODS: In this experimental study, the microarray dataset GSE33910, which includes expression data for peripheral blood mononuclear cell (PBMC) samples isolated from TA patients and normal volunteers, was downloaded from the publicly accessible Gene Expression Omnibus (GEO) database...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29100496/gene-expression-profiling-of-upregulated-mrnas-in-granulosa-cells-of-bovine-ovulatory-follicles-following-stimulation-with-hcg
#20
Jacques G Lussier, Mame N Diouf, Valérie Lévesque, Jean Sirois, Kalidou Ndiaye
BACKGROUND: Ovulation and luteinization of follicles are complex biological processes initiated by the preovulatory luteinizing hormone surge. The objective of this study was to identify genes that are differentially expressed in bovine granulosa cells (GC) of ovulatory follicles. METHODS: Granulosa cells were collected during the first follicular wave of the bovine estrous cycle from dominant follicles (DF) and from ovulatory follicles (OF) obtained 24 h following injection of human chorionic gonadotropin (hCG)...
November 3, 2017: Reproductive Biology and Endocrinology: RB&E
keyword
keyword
95716
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"