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Actinomyces

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https://www.readbyqxmd.com/read/28225785/betel-nut-chewing-oral-premalignant-lesions-and-the-oral-microbiome
#1
Brenda Y Hernandez, Xuemei Zhu, Marc T Goodman, Robert Gatewood, Paul Mendiola, Katrina Quinata, Yvette C Paulino
Oral cancers are attributed to a number of causal agents including tobacco, alcohol, human papillomavirus (HPV), and areca (betel) nut. Although betel nut chewing has been established as an independent cause of oral cancer, the mechanisms of carcinogenesis are poorly understood. An investigation was undertaken to evaluate the influence of betel nut chewing on the oral microbiome and oral premalignant lesions. Study participants were recruited from a dental clinic in Guam. Structured interviews and oral examinations were performed...
2017: PloS One
https://www.readbyqxmd.com/read/28218631/the-non-innoculous-hilar-calcification-recurrent-pneumonia-secondary-to-broncholith-associated-actinomyces
#2
Oluwayemisi Ojemakinde, Adam Wellikoff, Catherine Chaudoir, Guillermo Sangster, Alberto Simoncini, Carlos Previgliano, Disha Adelle Desouza
Dystrophic calcification of hilar lymph nodes is a common response to chronic inflammation related to several etiologies and rarely is associated with any clinical findings. A clinical scenario related to these calcified lymph nodes can thus be delayed by the low clinical suspicion associated with such a presumably innocuous finding. Normal respiratory movements however, can cause erosion into adjacent bronchi leading to a broncholith, complications of which can result in morbidity. We illustrate one of these complications, a partial obstruction with subsequent recurrent infection due to normal oral flora - actinomyces...
January 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28211942/antifungal-activity-change-of-streptomyces-rimosus-my02-mediated-by-confront-culture-with-other-microorganism
#3
Jicheng Yu, Qiu Liu, Chao Chen, Xiaohui Qi
Streptomyces rimosus can produce antibacterial and antifungal antibiotics, which have important applications in medicine and agriculture. Seventy-nine microbial strains were employed to assay interaction between S. rimosus MY02 and different fungi, actinomyces, and bacteria when confront cultured on solid media. The results showed that the presence of a microorganism might affect the activity of another one. When S. rimosus MY02 confront cultured with other microorganisms, the inductive effect might be positive or negative...
February 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28209819/draft-genome-sequence-of-actinomyces-glycerinitolerans-strain-g10-t-isolated-from-sheep-rumen-fluid
#4
Susakul Palakawong Na Ayudthaya, Nikolaos Strepis, Peter Pristaš, Caroline M Plugge
Actinomyces glycerinitolerans strain G10(T), which was isolated from sheep rumen fluid, can metabolize a range of substrates, including complex carbohydrates to organic acids (OAs). Here, we report a 3.69-Mbp draft genome of Actinomyces glycerinitolerans.
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28199766/dysbiosis-of-oral-buccal-mucosa-microbiota-in-patients-with-oral-lichen-planus
#5
Yuan He, Diping Gong, Cong Shi, Fangyang Shao, Jiahao Shi, Jian Fei
OBJECTIVE: The bacterial community structure of buccal mucosa in patients with oral lichen planus was evaluated and compared with healthy control. SUBJECTS AND METHODS: Buccal scraping samples have been taken on 43 oral lichen planus patients (21 erosive and 22 non-erosive) and 21 mucosal healthy volunteers. The V3 hypervariable 16S rDNA region were amplified and sequenced by high-throughput 454 pyrosequencing. RESULTS: 94.26% of the total buccal bacteria were classified into 15 abundant genera...
February 15, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28193569/analysis-of-the-expression-of-nlrp3-and-aim2-in-periapical-lesions-with-apical-periodontitis-and-microbial-analysis-outside-the-apical-segment-of-teeth
#6
Shujun Ran, Bin Liu, Shensheng Gu, Zhe Sun, Jingping Liang
OBJECTIVE: To detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and the absent in Melanoma 2 (AIM2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. DESIGN: The distribution of NLRP3 and AIM2 inflammasomes in sixteen periapical lesions was investigated by immunohistochemistry. Meanwhile, the relative gene expression levels of NLRP3 and AIM2 in sixteen periapical lesions and three health periodontal tissue were quantified by real-time polymerase chain reaction (PCR)...
February 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28188167/hidradenitis-suppurativa-neutrophilic-dermatoses-and-diverticular-disease-in-a-young-african-american-patient
#7
Kavita Darji, Maulik M Dhandha, Mary Guo
Hidradenitis suppurativa (HS) is a chronic skin disorder of the terminal follicular epithelium of apocrine sweat glands, manifesting as painful and exudative papules, pustules, cysts or nodules. This inflammatory condition often presents with other systemic and cutaneous disorders. We present the case of an African-American man with HS who was also diagnosed with neutrophilic dermatoses and diverticular disease. Neutrophilic dermatosis was identified based on histopathology findings. Our patient underwent multiple surgeries for flaring of his skin condition...
February 10, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28177125/association-of-oral-microbiome-with-type-2-diabetes-risk
#8
J Long, Q Cai, M Steinwandel, M K Hargreaves, S R Bordenstein, W J Blot, W Zheng, X O Shu
BACKGROUND AND OBJECTIVE: The oral microbiome may help to maintain systemic health, including how it affects blood glucose levels; however, direct evidence linking the oral microbiome with diabetes is lacking. MATERIAL AND METHODS: We compared the oral microbiome profiles of 98 participants with incident diabetes, 99 obese non-diabetics and 97 normal weight non-diabetics, via deep sequencing of the 16S rRNA gene. RESULTS: We found that the phylum Actinobacteria was present significantly less abundant among patients with diabetes than among the controls (p = 3...
February 8, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28173873/microbiomic-differences-in-tumor-and-paired-normal-tissue-in-head-and-neck-squamous-cell-carcinomas
#9
Hannah Wang, Pauline Funchain, Gurkan Bebek, Jessica Altemus, Huan Zhang, Farshad Niazi, Charissa Peterson, Walter T Lee, Brian B Burkey, Charis Eng
BACKGROUND: While the role of the gut microbiome in inflammation and colorectal cancers has received much recent attention, there are few data to support an association between the oral microbiome and head and neck squamous cell carcinomas. Prior investigations have been limited to comparisons of microbiota obtained from surface swabs of the oral cavity. This study aims to identify microbiomic differences in paired tumor and non-tumor tissue samples in a large group of 121 patients with head and neck squamous cell carcinomas and correlate these differences with clinical-pathologic features...
February 7, 2017: Genome Medicine
https://www.readbyqxmd.com/read/28169034/diagnosis-of-actinomyces-graevenitzii-lung-infection-using-linear-ebus
#10
Alberto Caballero Vázquez, Juan Jose Cruz Rueda, Julián Andrés Ceballos Gutierrez
No abstract text is available yet for this article.
February 3, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/28162845/role-of-microbiological-culture-and-polymerase-chain-reaction-pcr-of-actinomyces-in-medication-related-osteonecrosis-of-the-jaw-mronj
#11
Sappasith Panya, Riham Fliefel, Florian Probst, Matthias Tröltzsch, Michael Ehrenfeld, Sören Schubert, Sven Otto
We hypothesized that local infection plays a critical role in the pathogenesis of medication-related osteonecrosis of the jaw (MRONJ). Recent developments in molecular methods have revolutionized new approaches for the rapid detection of microorganisms including those difficult to culture. The aim of our study is to identify the bacterial profiles in MRONJ by microbiological culture and polymerase chain reactions (PCR). A retrospective analysis was performed on MRONJ patients from 2008 to 2014. The bacterial profile from MRONJ bone samples was determined using microbiological culture and PCR...
January 9, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28159588/bacterial-diversity-in-medication-related-osteonecrosis-of-the-jaw
#12
Fredrik Hallmer, Tore Bjørnland, Gunilla Andersson, Jonas P Becktor, Anne K Kristoffersen, Morten Enersen
OBJECTIVE: The aim was to study the association between microflora and medication-related osteonecrosis of the jaw (MRONJ) by using culture-independent molecular techniques to detect bacteria in necrotic bone lesions. STUDY DESIGN: Included were 18 consecutive patients with MRONJ, 10 with osteoporosis and 8 cancer patients. Bone biopsies were retrieved from the center of the necrotic bone and from visually healthy bone, and 16 S rRNA gene fragments from bacterial DNA were amplified with polymerase chain reaction...
December 7, 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28141637/cervicofacial-primary-cutaneous-actinomycosis-surgical-treatment-for-complete-remission-of-the-disease
#13
Yeon Jin Jeong, Hyo Wan Suh, Hyung-Sup Shim
Actinomycosis, an infectious bacterial disease caused by Actinomyces species, is very rare and is characterized by contiguous spreading, subacute to chronic granulomatous inflammation and the formation of multiple abscesses and sinus tracts that may discharge sulfur granules. Actinomycosis that presents on the skin without endogenous origin is called primary cutaneous actinomycosis, and the occurrence and treatment of primary cutaneous actinomycosis is rarely reported. This report describes the treatment of primary cervicofacial actinomycosis with a literature review, and emphasizes the importance of surgical option for complete remission of the disease...
January 30, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28131960/three-species-biofilm-model-onto-plasma-treated-titanium-implant-surface
#14
Adaias O Matos, Antônio P Ricomini-Filho, Thamara Beline, Erika S Ogawa, Bárbara E Costa-Oliveira, Amanda B de Almeida, Francisco H Nociti Junior, Elidiane C Rangel, Nilson C da Cruz, Cortino Sukotjo, Mathew T Mathew, Valentim A R Barão
In this study, titanium (Ti) was modified with biofunctional and novel surface by micro-arc oxidation (MAO) and glow discharge plasma (GDP) and we tested the development of a three-species periodontopatogenic biofilm onto the treated commercially-pure titanium (cpTi) surfaces. Machined and sandblasted surfaces were used as control group. Several techniques for surface characterizations and monoculture on bone tissue cells were performed. A multispecies biofilm composed of Streptococcus sanguinis, Actinomyces naeslundii and Fusobacterium nucleatum was developed onto cpTi discs for 16...
January 21, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28122873/rising-stakes-for-healthcare-associated-infection-prevention-implications-for-the-clinical-microbiology-laboratory-revised
#15
Daniel J Diekema
Healthcare associated infection (HAI) rates are subject to public reporting, and are linked to hospital reimbursement from the Centers for Medicare and Medicaid Services (CMS). The increasing pressure to lower HAI rates comes at a time when advances in the clinical microbiology laboratory (CML) provide more precise and sensitive tests, altering HAI detection in ways that may increase reported HAI rates. I review how changing CML practices can impact HAI rates, and how the financial implications of HAI metrics may produce pressure to change diagnostic testing practices...
January 25, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28115186/red-fluorescence-of-dental-plaque-in-children-a-cross-sectional-study
#16
Catherine M C Volgenant, Egija Zaura, Bernd W Brandt, Mark J Buijs, Marisol Tellez, Gayatri Malik, Amid I Ismail, Jacob M Ten Cate, Monique H van der Veen
OBJECTIVES: The relation between the presence of red fluorescent plaque and the caries status in children was studied. In addition, the microbial composition of dental plaque from sites with red fluorescent plaque (RFP) and from sites with no red fluorescent plaque (NFP) was assessed. METHODS: Fluorescence photographs were taken from fifty children (6-14 years old) with overnight plaque. Full-mouth caries scores (ICDAS II) were obtained. The composition of a saliva sample and two plaque samples (RFP and NFP) was assessed using 16S rDNA sequencing...
January 20, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28109846/antimicrobial-susceptibility-of-clinical-isolates-of-actinomyces-and-related-genera-reveals-an-unusual-clindamycin-resistance-among-actinomyces-urogenitalis-strains
#17
Claudia Barberis, Mabel Budia, Susana Palombarani, Carlos Hernán Rodriguez, María Soledad Ramírez, Barbara Arias, Laura Bonofiglio, Angela Famiglietti, Marta Mollerach, Marisa Almuzara, Carlos Vay
OBJECTIVES: Patterns of antimicrobial susceptibility in Actinomyces and related genera are very limited in the literature. Data of predominant susceptibility profiles could contribute to the establishment of an accurate empirical treatment. METHODS: A total of 113 isolates from clinical samples were included in this study. Each isolate was identified using phenotypic methods and MALDI-TOF/MS. When discrepancies were observed, 16S rRNA gene sequencing was performed...
January 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28108235/the-use-of-bacteriophages-to-biocontrol-oral-biofilms
#18
Szymon P Szafrański, Andreas Winkel, Meike Stiesch
Infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. As bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. An innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and can penetrate into biofilms...
January 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28065676/rhamnolipids-from-non-pathogenic-burkholderia-thailandensis-e264-physicochemical-characterization-antimicrobial-and-antibiofilm-efficacy-against-oral-hygiene-related-pathogens
#19
Mohamed Elshikh, Scott Funston, Alif Chebbi, Syed Ahmed, Roger Marchant, Ibrahim M Banat
Biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. Rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. One of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportunistic pathogen Pseudomonas aeruginosa...
May 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28061510/bacterial-coaggregation-among-the-most-commonly-isolated-bacteria-from-contact-lens-cases
#20
Ananya Datta, Fiona Stapleton, Mark D P Willcox
Purpose: To examine the coaggregation and cohesion between the commonly isolated bacteria from contact lens cases. Methods: Four or five strains each of commonly isolated bacteria from contact lens cases, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Serratia marcescens, were grown, washed, mixed in equal proportions, and allowed to coaggregate for 24 hours. Lactose (0.06 M), sucrose (0.06 M), and pronase (2 mg/mL; 2 hours, 37°C) were used to inhibit coaggregation...
January 1, 2017: Investigative Ophthalmology & Visual Science
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