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https://www.readbyqxmd.com/read/27901206/antimicrobial-activity-of-different-disinfectants-against-cariogenic-microorganisms
#1
Esra Uzer Celik, Ayse Tugce Tunac, Mustafa Ates, Bilge Hakan Sen
The aim of this study was to assess the in vitro antimicrobial effects of chlorhexidine digluconate (CHX), polyhexamethylene biguanide (PHBM), and octenidine dihydrochloride (OCT) on cariogenic microorganisms by using their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). CHX, PHBM, and OCT were diluted in distilled water to the final test concentrations. Using the in-tube dilution method, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Actinomyces viscosus were cultivated on blood agar and Mueller-Hinton broth (MHB) at 37°C for 48 h...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27898021/salivary-microbiome-diversity-in-caries-free-and-caries-affected-children
#2
Shan Jiang, Xiaoli Gao, Lijian Jin, Edward C M Lo
Dental caries (tooth decay) is an infectious disease. Its etiology is not fully understood from the microbiological perspective. This study characterizes the diversity of microbial flora in the saliva of children with and without dental caries. Children (3-4 years old) with caries (n = 20) and without caries (n = 20) were recruited. Unstimulated saliva (2 mL) was collected from each child and the total microbial genomic DNA was extracted. DNA amplicons of the V3-V4 hypervariable region of the bacterial 16S rRNA gene were generated and subjected to Illumina Miseq sequencing...
November 25, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27896580/highly-improved-acarbose-production-of-actinomyces-through-the-combination-of-artp-and-penicillin-susceptible-mutant-screening
#3
Fei Ren, Long Chen, Qunyi Tong
Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27870811/abdominal-actinomycosis-in-children-a-case-report-and-literature-review
#4
Noppadol Wacharachaisurapol, Jeffrey M Bender, Larry Wang, David Bliss, Skorn Ponrartana, Pia S Pannaraj
Abdominal actinomycosis is an uncommon pediatric infection that often manifests with a tumor-like lesion. We describe a previously healthy 11-year-old girl who presented with right lower quadrant abdominal pain and drainage. Computed tomography scan showed an abdominal wall mass. Surgical debridement cultures grew Actinomyces meyeri. Literature review identified 18 additional pediatric cases since 1964 that we have summarized.
November 18, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27843558/prevalence-of-bovine-milk-pathogens-in-azorean-pastures-mobile-versus-fixed-milking-machines
#5
C Azevedo, D Pacheco, L Soares, M Moitoso, J Maldonado, R Guix, J Simões
The aims of the present study were (1) to evaluate the influence of using mobile (n=47) or fixed (n=45) milking machines in Azorean herds on the apparent prevalence of several milk pathogens in bulk tank milk (BTM) and (2) to determine whether separated subclinical mastitic cows can serve, in real time, as predictors of milk pathogen prevalence for the remaining animals at the herd level. The use of a mobile or fixed milking machine influenced (P≤0.05) the prevalence of Staphylococcus aureus (72.3 per cent; n=34 v 51...
2016: Veterinary Record Open
https://www.readbyqxmd.com/read/27837345/molecular-detection-of-bacteria-associated-to-caries-activity-in-dentinal-lesions
#6
Beatriz Gonçalves Neves, Rafael Nóbrega Stipp, Daniela da Silva Bezerra, Sarah Florindo de Figueiredo Guedes, Lidiany Karla Azevedo Rodrigues
OBJECTIVES: This study aimed at identifying and quantifying Actinomyces naeslundii, Bifidobacterium spp., Streptococcus mitis group, Lactobacillus acidophilus, Lactobacillus casei group, Streptococcus gordonii, and Streptococcus mutans in active and inactive carious dentine lesions of children with early childhood caries by using quantitative polymerase chain reaction. MATERIAL AND METHODS: Fifty-six dentin lesion samples, classified as active (n = 39) or inactive (n = 17), were collected from children aged from 2 to 5 years old...
November 12, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27834171/bacterial-communities-associated-with-apical-periodontitis-and-dental-implant-failure
#7
Simon Dingsdag, Stephen Nelson, Nicholas V Coleman
BACKGROUND: Previously, we demonstrated that bacteria reside in apparently healed alveolar bone, using culture and Sanger sequencing techniques. Bacteria in apparently healed alveolar bone may have a role in peri-implantitis and dental implant failure. OBJECTIVE: To compare bacterial communities associated with apical periodontitis, those colonising a failed implant and alveolar bone with reference biofilm samples from healthy teeth. METHODS AND RESULTS: The study consisted of 196 samples collected from 40 patients undergoing routine dental implant insertion or rehabilitation...
2016: Microbial Ecology in Health and Disease
https://www.readbyqxmd.com/read/27833819/salivary-microbiomes-of-indigenous-tsimane-mothers-and-infants-are-distinct-despite-frequent-premastication
#8
Cliff S Han, Melanie Ann Martin, Armand E K Dichosa, Ashlynn R Daughton, Seth Frietze, Hillard Kaplan, Michael D Gurven, Joe Alcock
BACKGROUND: Premastication, the transfer of pre-chewed food, is a common infant and young child feeding practice among the Tsimane, forager-horticulturalists living in the Bolivian Amazon. Research conducted primarily with Western populations has shown that infants harbor distinct oral microbiota from their mothers. Premastication, which is less common in these populations, may influence the colonization and maturation of infant oral microbiota, including via transmission of oral pathogens...
2016: PeerJ
https://www.readbyqxmd.com/read/27800254/canaliculitis-awareness
#9
Melike Balıkoğlu Yılmaz, Emine Şen, Ebru Evren, Ufuk Elgin, Pelin Yılmazbaş
OBJECTIVES: To evaluate the demographic characteristics, treatment, and results of patients with canaliculitis. MATERIALS AND METHODS: Medical records including the demographic characteristics, clinical findings, and treatment outcomes of patients diagnosed and treated for canaliculitis between September 2009 and March 2014 were analyzed retrospectively. RESULTS: The median age of the 7 canaliculitis patients consisting of 4 women and 3 men was 49 (range 8-58) years...
January 2016: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/27789164/-a-case-of-mediastinum-actinomycosis-by-aggregatibacter-actinomycetemcomitans
#10
N N M Razafimanjato, A M Portela, D M Radu, P Guiraudet, M D Destable, A Seguin, E Martinod
The actinomycosis is a suppurative infection due to an anaerobic and microaerophillic bacteria called actinomyces. Only few case reports are described for the mediastinal locations of this rare entity. We report a new case of inflammatory pseudotumor in the mediastinum due to Aggregatibacte actinomycetemcomitans revealed by hemoptysis. The mediastinoscopy procedure with biopsy was needed to confirm the definitive bacteriological diagnosis by a positive culture. During the postoperative course, a cutaneous fistula was found which had a favourable evolution after appropriate antibiotherapy...
October 24, 2016: Revue de Pneumologie Clinique
https://www.readbyqxmd.com/read/27775308/-appendicular-actinomycosis-as-a-cause-of-ileocecal-mass-in-children-case-report
#11
M Fernández Castilla, A Salazar Villacorta, A Martín Nalda, J C Ferreres Piñas, A Coma Muñoz, G Guillén Burrieza
INTRODUCTION: Appendicular actinomycosis is a rare disease produced by cecal appendix invasion by Actinomyces gender bacteria after a predisposing cause that disrupts mucosal barrier, such as acute appendicitis. The highest frequency appears in adults; however, there are cases in paediatric age described in literature. The aim of this article is to introduce a case of appendicular actinomycosis in a child with atypical presentation and to review the literature. CLINICAL CASE: 10-year-old boy transferred to our hospital due to intraoperative finding at a different centre of a right flank tumor, without identification of cecal appendix...
July 20, 2015: Cirugía Pediátrica: Organo Oficial de la Sociedad Española de Cirugía Pediátrica
https://www.readbyqxmd.com/read/27771826/the-mycobiome-of-root-canal-infections-is-correlated-to-the-bacteriome
#12
Ilona F Persoon, Mark J Buijs, Ahmet R Özok, Wim Crielaard, Bastiaan P Krom, Egija Zaura, Bernd W Brandt
OBJECTIVES: Bacterial infection of the root canal system causes apical periodontitis. Less is known about the role of fungi in these infections. This study aimed to assess the fungal prevalence, abundance, and diversity of root canal infections, as well as the relation between fungi and bacteria present in different parts of the root canal. MATERIALS AND METHODS: Twenty-six teeth with primary apical periodontitis were extracted, split in apical and coronal root segments, and cryo-pulverized...
October 22, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27769339/case-of-actinomyces-lacrimal-sac-abscess-mimicking-malignancy
#13
R Tom Liu, Lucy Goold, Valerie A White, Steve Rasmussen, Vivian T Yin
No abstract text is available yet for this article.
October 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/27759406/salivary-microbiota-and-caries-occurrence-in-mutans-streptococci-positive-school-children
#14
M ElSalhy, E Söderling, E Honkala, M Fontana, S Flannagan, A Kokaras, B J Paster, A Varghese, S Honkala
AIM: To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition. MATERIALS AND METHODS: Twenty eight healthy, 11-12-year-old schoolchildren with high MS counts (>10⊃5 CFU/mL) were included in this study. The children were screened with the Dentocult SM Strip Mutans test (Orion Diagnostica, Espoo, Finland) and examined using the International Caries Detection and Assessment System (ICDAS)...
September 2016: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/27752374/empyema-secondary-to-actinomyces-meyeri-treated-successfully-with-ceftriaxone-followed-by-doxycycline
#15
Etienne Paris, Tonio Piscopo, Karen Cassar
Actinomycosis is a relatively rare infection caused by Gram-positive bacteria. We present the case of a 54-year-old, previously healthy, male patient with a history of severe penicillin allergy who developed severe pneumonia and empyema caused by Actinomyces meyeri. Presenting symptoms included productive cough, right upper quadrant pain, and chills and rigors. He required drainage of the empyema via tube and prolonged antibiotic treatment with intravenous ceftriaxone for 2 weeks followed by oral doxycycline for 6 months...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27730039/primary-actinomycosis-of-vulva-with-inguinal-lymphadenopathy
#16
Anand J Asia, Vaibhav N Tapre
Actinomycosis is a chronic suppurative granulomatous infection of subcutaneous tissues caused by bacterium Actinomyces israelii. It is a normal commensal of the oral cavity, gastrointestinal tract, respiratory tract, and vagina. Infection is first established locally by breach of mucosal barrier during various procedures, aspiration, trauma, or human bite. Rarely, it may spread through hematogenous and lymphatic system. We present a case of actinomycosis involving the vulva, extending to the inguinal region along with inguinal lymphadenopathy...
September 2016: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/27722787/bacterial-invasion-into-radicular-dentine-an-in-vitro-study
#17
Simone Stauffacher, Adrian Lussi, Sandor Nietzsche, Klaus W Neuhaus, Sigrun Eick
OBJECTIVES: We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals. METHODS: Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordonii ATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5)...
October 8, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27701899/the-effect-of-seasons-on-brazilian-red-propolis-and-its-botanical-source-chemical-composition-and-antibacterial-activity
#18
Bruno Bueno-Silva, Alexandre Marsola, Masaharu Ikegaki, Severino M Alencar, Pedro L Rosalen
The aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of Brazilian red propolis (BRP) and its plant source. BRP was collected from Maceio, Alagoas state, north-east of Brazil, during one year. Chemical composition was determined by physicochemical analyses and HPLC while antimicrobial activity was assessed against Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus and Actinomyces naeslundii by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC, respectively)...
October 4, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27666996/supragingival-plaque-microbial-community-analysis-of-children-with-halitosis
#19
Wen Ren, Qun Zhang, Xuenan Liu, Shuguo Zheng, Lili Ma, Feng Chen, Tao Xu, Baohua Xu
As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present...
September 23, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27660886/benefits-of-bifidobacterium-animalis-subsp-lactis-probiotic-in-experimental-periodontitis
#20
Luiz F F Oliveira, Sérgio L Salvador, Pedro H F Silva, Flávia A C Furlaneto, Luciene Figueiredo, Renato Casarin, Edilson Ervolino, Daniela B Palioto, Sérgio L S Souza, Mario Taba, Arthur B Novaes, Michel R Messora
BACKGROUND: This study evaluated the effects of topical administration of probiotic bacteria (PROB) of the genus Bifidobacterium on experimental periodontitis (EP) in rats. METHODS: 32 rats were divided into groups C (control; without EP), EP (EP only), C-HN019 (control+PROB) and EP-HN019 (EP+PROB). On day 0 of the experiment, animals of groups EP and EP-HN019 received cotton ligatures around mandibular first molars (MFM). In groups C-HN019 and EP-HN019, 1mL of suspensions containing Bifidobacterium animalis subsp lactis (B...
September 23, 2016: Journal of Periodontology
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