Read by QxMD icon Read


Xin Quan, Liangliang Huang, Yafeng Yang, Teng Ma, Zhongyang Liu, Jun Ge, Jinghui Huang, Zhuojing Luo
Electrical stimulation (ES) promotes neurite outgrowth and nerve regeneration, but the underlying mechanisms remain undefined. In the present study, we investigated the role of micro RNAs (miRNAs) in ES-mediated neurite outgrowth. First, we performed microarray analyses to identify changes in the miRNAs profile of dorsal root ganglion neurons (DRGNs) following ES. The expression of 16 known miRNAs was altered by ES. Bioinformatics showed that the potential targets of these differentially expressed miRNAs were involved in neurite outgrowth...
November 30, 2016: Neurochemical Research
Parthasarathy Chandrakesan, Randal May, Nathaniel Weygant, Dongfeng Qu, William L Berry, Sripathi M Sureban, Naushad Ali, Chinthalapally Rao, Mark Huycke, Michael S Bronze, Courtney W Houchen
Crypt epithelial survival and regeneration after injury require highly coordinated complex interplay between resident stem cells and diverse cell types. The function of Dclk1 expressing tuft cells regulating intestinal epithelial DNA damage response for cell survival/self-renewal after radiation-induced injury is unclear. Intestinal epithelial cells (IECs) were isolated and purified and utilized for experimental analysis. We found that small intestinal crypts of Villin(Cre);Dclk1(f/f) mice were hypoplastic and more apoptotic 24 h post-total body irradiation, a time when stem cell survival is p53-independent...
November 23, 2016: Scientific Reports
Andrew O'Neil, Christine P Petersen, Eunyoung Choi, Amy C Engevik, James R Goldenring
The glandular stomach has two major zones: the acid secreting corpus and the gastrin cell-containing antrum. Nevertheless, a single gland lies at the transition between the forestomach and corpus in the mouse stomach. We have sought to define the lineages that make up this gland unit at the squamocolumnar junction. The first gland in mice showed a notable absence of characteristic corpus lineages, including parietal cells and chief cells. In contrast, the gland showed strong staining of Griffonia simplicifolia-II (GSII)-lectin-positive mucous cells at the bases of glands, which were also positive for CD44 variant 9 and Clusterin...
November 21, 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
C Benedikt Westphalen, Moritz Middelhoff, Michael Quante, Timothy C Wang
No abstract text is available yet for this article.
2016: Stem Cell Investigation
Raphael Schellnegger, Anne Quante, Susanne Rospleszcz, Martina Schernhammer, Bettina Höhl, Moritz Tobiasch, Agnieszka Pastula, Anna Brandtner, Julian A Abrams, Konstantin Strauch, Roland M Schmid, Michael Vieth, Timothy C Wang, Michael Quante
The increasing incidence of esophageal adenocarcinoma (EAC) is mirrored by the increasing prevalence of Barrett esophagus, a precursor lesion resulting in a large number of individuals "at risk" for this lethal malignancy. Among patients with Barrett esophagus, only about 0.3% annually will develop EAC. Because large numbers of patients are followed in endoscopic surveillance, there is a need for risk prediction among a growing population of patients with Barrett esophagus. We identified four potential biomarkers from an inflammation (IL1β)-dependent mouse model of Barrett esophagus and tested them in 189 patients with Barrett esophagus with and without high-grade dysplasia (HGD)/early cancer (T1)...
November 2, 2016: Cancer Prevention Research
Alireza Mirzaei, Zahra Madjd, Azade Amini Kadijani, Masoumeh Tavakoli-Yaraki, Mohammad Hossein Modarresi, Javad Verdi, Abolfazl Akbari, Gholamreza Tavoosidana
BACKGROUND: DCLK1, as the most potential colorectal cancer stem cell (CSC) marker has been the core of many recent investigations. However, no study has been performed to evaluate the circulating cellular DCLK1 protein (CCDP) that might reflect the presences of colorectal CSC in circulation. OBJECTIVES: We aimed to evaluate CCDP in the peripheral blood mononuclear cells (PBMCs) of colorectal cancer (CRC) patients applying immunoassay based methods including PLA, IPCR and ELISA in order to introduce the method of choice for clinical detection of CCDP...
September 26, 2016: Cancer Biomarkers: Section A of Disease Markers
Pomila Singh, Malaney O'Connell, Sarkar Shubhashish
Colorectal carcinogenesis is a multi-step process. While ~25% of colorectal cancers (CRCs) arise in patients with a family history (genetic predisposition), ~75% of CRCs are due to age-associated accumulation of epigenetic alterations which can result in the suppression of key tumor suppressor genes leading to mutations and activation of oncogenic pathways. Sporadic colon-carcinogenesis is facilitated by many molecular pathways of genomic instability which include chromosomal instability (CIN), micro-satellite instability (MSI) and CpG island methylator phenotype (CIMP), leading towards loss of homeostasis and onset of neoplastic transformation...
2016: Stem Cell Investigation
Charles B Nguyen, Courtney W Houchen, Naushad Ali
Liver diseases are the fourth leading cause of mortality among adults in the United States. Patients with chronic liver diseases such as viral hepatitis, fibrosis, and cirrhosis have significantly higher risks of developing hepatocellular carcinoma (HCC). With a dismal five-year survival rate of 11%, HCC is the third most common cause of cancer-related deaths worldwide. Regardless of the underlying cause, late presentation and a lack of effective therapy are the major impediments for successful treatment of HCC...
September 30, 2016: Experimental Biology and Medicine
Joni Leppänen, Olli Helminen, Heikki Huhta, Joonas H Kauppila, Ilkka Miinalainen, Veli-Pekka Ronkainen, Juha Saarnio, Petri P Lehenkari, Tuomo J Karttunen
Doublecortin-like kinase 1 (DCLK1) is a microtubule-associated kinase. In murine intestine, DCLK1 marks tuft cells with characteristic microvilli, features of neuroendocrine cells and also quiescent stem cell-like properties. The occurrence and pathological role of DCLK1-positive cells in human intestinal mucosa is unknown. We analysed DCLK1 expression in healthy duodenal, jejunal and colorectal mucosa samples (n = 35), and in duodenal specimens from patients with coeliac disease (n = 20). The samples were immunohistochemically double-stained with DCLK1, and synaptophysin, chromogranin A and Ki-67...
November 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Hironori Koga, Yu Ikezono, Takuji Torimura
No abstract text is available yet for this article.
2016: Stem Cell Investigation
Onisha Patel, Weiwen Dai, Mareike Mentzel, Michael D W Griffin, Juliette Serindoux, Yoann Gay, Stefanie Fischer, Shoukat Sterle, Ashleigh Kropp, Christopher J Burns, Matthias Ernst, Michael Buchert, Isabelle S Lucet
Doublecortin-like kinase 1 (DCLK1) is a serine/threonine kinase that belongs to the family of microtubule-associated proteins. Originally identified for its role in neurogenesis, DCLK1 has recently been shown to regulate biological processes outside of the CNS. DCLK1 is among the 15 most common putative driver genes for gastric cancers and is highly mutated across various other human cancers. However, our present understanding of how DCLK1 dysfunction leads to tumorigenesis is limited. Here, we provide evidence that DCLK1 kinase activity negatively regulates microtubule polymerization...
September 6, 2016: Structure
Matthew P Hanley, Krishna Kadaveru, Christine Perret, Charles Giardina, Daniel W Rosenberg
The role of folate one-carbon metabolism in colorectal cancer development is controversial, with nutritional intervention studies producing conflicting results. It has been reported that Apc(Min/+) mice maintained on a diet deficient in the methyl donors folic acid, methionine, choline, and vitamin B12, and supplemented with homocysteine, show a greater than 95% reduction in intestinal tumor development. The present study extends these findings and shows that tumor protection afforded by dietary methyl donor deficiency (MDD) is long-lasting...
October 2016: Cancer Prevention Research
Tianbo Gao, Min Wang, Lingling Xu, Tao Wen, Jian Liu, Guangyu An
PURPOSE: Metastasis is a primary cause of colorectal cancer (CRC)-related death, and cancer stem cells (CSCs) are thought to be majorly responsible for initiating metastatic behaviors. Doublecortin-like kinase 1 (DCLK1) was recently discovered to be a marker for gastrointestinal CSCs. Here, we aimed to explore whether DCLK1 is associated with CRC metastasis through clinical and in vitro investigations. METHODS: The expression levels of DCLK1 mRNA and protein in human CRC tissues were analyzed through quantitative RT-PCR and immunohistochemistry staining, respectively...
October 2016: Journal of Cancer Research and Clinical Oncology
Hiroyuki Koizumi, Hiromi Fujioka, Kazuya Togashi, James Thompson, John R Yates, Joseph G Gleeson, Kazuo Emoto
Doublecortin-like kinase 1 (DCLK1) is a member of the neuronal microtubule-associated doublecortin (DCX) family and functions in multiple stages of neural development including radial migration and axon growth of cortical neurons. DCLK1 is suggested to play the roles in part through its protein kinase activity, yet the kinase substrates of DCLK1 remain largely unknown. Here we have identified MAP7D1 (microtubule-associated protein 7 domain containing 1) as a novel substrate of DCLK1 by using proteomic analysis...
August 9, 2016: Developmental Neurobiology
Lorenz Kadletz, Klaus Aumayr, Gregor Heiduschka, Sven Schneider, Elisabeth Enzenhofer, Claudia Lill
Salivary gland carcinomas are a rare malignancy. Therefore, little is known about biomarkers and cancer stem cells in salivary gland malignancies. Double cortin-like kinase 1 (DCLK1) is a promising therapeutic target and cancer stem cell marker, predominantly investigated in pancreatic and colorectal cancer. The purpose of this study was to investigate the expression of DCLK1 in major and minor salivary gland carcinomas and its influence on survival. We examined a total of 80 patients with major or minor salivary gland cancer in this retrospective study...
July 28, 2016: European Archives of Oto-rhino-laryngology
C Benedikt Westphalen, Michael Quante, Timothy C Wang
Doublecortin like kinase protein 1 (Dclk1) is a microtubule-associated protein with C-terminal serine/threonine kinase domain. Originally designated Doublecortin and CaM kinase-like 1 protein (Dcamkl1) or KIAA0369, Dclk1 was first described as a marker for radial glia cells in the context of microtubule polymerization and neuronal migration, possibly contributing to early neurogenesis. Additionally, Dclk1 was proposed as a marker of quiescent gastrointestinal and pancreatic stem cells, but in recent years has been recognized as a marker for tuft cells in the gastrointestinal tract...
July 26, 2016: Small GTPases
Kishan Bellamkonda, Naveen Kumar Chandrashekar, Janina Osman, Benson Chellakkan Selvanesan, Sayeh Savari, Anita Sjölander
BACKGROUND: Colorectal cancer is one of the most common types of cancers worldwide. Recent studies have identified cancer-initiating cells (CICs) as a subgroup of replication-competent cells in the development of colorectal cancer. Although it is understood that an inflammation-rich tumor microenvironment presumably supports CIC functions, the contributory factors are not very well defined. The present study advances our understanding of the role of the eicosanoids leukotriene D4 (LTD4) and prostaglandin E2 (PGE2) in the tumorigenic ability of CICs and investigates the consequential changes occurring in the tumor environment that might support tumor growth...
2016: BMC Cancer
P Chandrakesan, J Panneerselvam, D Qu, N Weygant, R May, M S Bronze, C W Houchen
The identification of functionally relevant subpopulations of therapy-resistant cancer cells is a challenge. These cells, intrinsically resistant to conventional therapy, can cause recurrence. Evidence has suggested that therapy-resistant cancer cells are likely epithelial-mesenchymal transition (EMT) cells and/or stem-like cells called cancer stem cells (CSCs). EMT, a normal embryological process that converts epithelial cells into mesenchymal cells, is frequently activated during cancer development and progression...
April 2016: Journal of Carcinogenesis & Mutagenesis
C Benedikt Westphalen, Yoshihiro Takemoto, Takayuki Tanaka, Marina Macchini, Zhengyu Jiang, Bernhard W Renz, Xiaowei Chen, Steffen Ormanns, Karan Nagar, Yagnesh Tailor, Randal May, Youngjin Cho, Samuel Asfaha, Daniel L Worthley, Yoku Hayakawa, Aleksandra M Urbanska, Michael Quante, Maximilian Reichert, Joshua Broyde, Prem S Subramaniam, Helen Remotti, Gloria H Su, Anil K Rustgi, Richard A Friedman, Barry Honig, Andrea Califano, Courtney W Houchen, Kenneth P Olive, Timothy C Wang
The existence of adult pancreatic progenitor cells has been debated. While some favor the concept of facultative progenitors involved in homeostasis and repair, neither a location nor markers for such cells have been defined. Using genetic lineage tracing, we show that Doublecortin-like kinase-1 (Dclk1) labels a rare population of long-lived, quiescent pancreatic cells. In vitro, Dclk1+ cells proliferate readily and sustain pancreatic organoid growth. In vivo, Dclk1+ cells are necessary for pancreatic regeneration following injury and chronic inflammation...
April 7, 2016: Cell Stem Cell
Wenyao Wang, Hongfei Zhang, Lichao Wang, Shaojun Zhang, Miao Tang
microRNAs (miRNAs) play key regulatory roles in various biological processes. In this study, we aimed to determine the expression and biological roles of miR-613 in hepatocellular carcinoma (HCC). Compared with non-cancerous liver tissues, miR-613 was significantly downregulated in HCC tissues. Ectopic expression of miR-613 significantly suppressed the proliferation and invasion of Hep3B and SMMC-7721 HCC cells. Bioinformatic and luciferase reporter analysis identified doublecortin-like kinase 1 (DCLK1) as a direct target of miR-613...
May 13, 2016: Biochemical and Biophysical Research Communications
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"