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https://www.readbyqxmd.com/read/29871972/mir-362-3p-functions-as-a-tumor-suppressor-through-targeting-mcm5-in-cervical-adenocarcinoma
#1
Dan Wang, Hongyan Wang, Yichun Li, Qian Li
Our previous study suggested that minichromosome maintenance protein 5 (MCM5) overexpression was observed in cervical adenocarcinoma, and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade and poor prognosis. Down-regulation of MCM5 inhibited cervical adenocarcinoma cell proliferation. The purpose of this study is to search and confirm valuable microRNAs (miRNAs), which target MCM5 to modulate cervical adenocarcinoma cell proliferation...
June 5, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29656186/dclk1-plays-an-important-role-in-colorectal-cancer-tumorgenesis-through-the-regulation-of-mir-200c
#2
Yasaman Mohammadi, Seyed Mohammad Tavangar, Massoud Saidijam, Razieh Amini, Katayoon Etemadi, Fatemeh Karimi Dermani, Rezvan Najafi
Doublecortin-like kinase 1 (DCLK1) is a protein kinase that is known as a specific cancer stem cell (CSC) marker in colorectal cancer (CRC). Deregulation of DCLK1 expression has been reported in various cancers. We measured the protein expression of DCLK1 in 38 CRC and normal colon samples by immunohistochemistry (IHC). HCT-116 and SW-48 cells were transfected with DCLK1 siRNA and analyzed for expression of DCLK1 and miR-200c. The effects of DCLK1 knockdown on cell migration, invasion, sphere-forming, and apoptosis were explored...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29604293/immune-cell-production-of-interleukin-17-induces-stem-cell-features-of-pancreatic-intraepithelial-neoplasia-cells
#3
Yu Zhang, Michelle Zoltan, Erick Riquelme, Hanwen Xu, Ismet Sahin, Susana Castro-Pando, Maria Fernanda Montiel, Kyle Chang, Zhengyu Jiang, Jianhua Ling, Sonal Gupta, William Horne, Melissa Pruski, Huamin Wang, Shao-Cong Sun, Guillermina Lozano, Paul Chiao, Anirban Maitra, Steven D Leach, Jay K Kolls, Eduardo Vilar Sanchez, Timothy C Wang, Jennifer M Bailey, Florencia McAllister
BACKGROUND & AIMS: Little is known about how the immune system affects stem cell features of pancreatic cancer cells. Immune cells that produce interleukin 17A (IL17A) in the chronically inflamed pancreas (chronic pancreatitis) contribute to pancreatic interepithelial neoplasia (PanIN) initiation and progression. We investigated the effects that IL17A signaling exerts on pancreatic cancer progenitor cells and the clinical relevance of this phenomena. METHODS: We performed studies with Mist1Cre;LSLKras;Rosa26mTmG (KCiMist ;G) and Kras(G12D);Trp53(R172H);Pdx1-Cre (KPC) mice (which upon tamoxifen induction spontaneously develop PanINs) and control littermates...
March 29, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29577277/alternative-splice-variants-of-dclk1-mark-cancer-stem-cells-promote-self-renewal-and-drug-resistance-and-can-be-targeted-to-inhibit-tumorigenesis-in-kidney-cancer
#4
Yang Ge, Nathaniel Weygant, Dongfeng Qu, Randal May, William L Berry, Jiannan Yao, Parthasarathy Chandrakesan, Wei Zheng, Lichao Zhao, Karena L Zhao, Michael Drake, Kenneth J Vega, Michael S Bronze, James J Tomasek, Guangyu An, Courtney W Houchen
Renal cell carcinoma (RCC) is a common and devastating disease characterized by a hypoxic microenvironment, epithelial-mesenchymal transition and potent resistance to therapy evidencing the presence of cancer stem cells (CSCs). Various CSC markers have been studied in RCC, but overall there is limited data on their role and most markers studied have been relatively nonspecific. Doublecortin-like kinase 1 (DCLK1) is a validated CSC marker in the gastrointestinal tract and evidence for an equivalent role in other cancers is accumulating...
March 25, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29550638/mir-424-5p-regulates-cell-proliferation-migration-and-invasion-by-targeting-doublecortin-like-kinase-1-in-basal-like-breast-cancer
#5
Jianling Wang, Shibing Wang, Jijun Zhou, Qian Qian
Our previous study has showed doublecortin like kinase 1 (DCLK1) serves as an oncogene to regulate basal-like breast cancer cell proliferation, migration and invasion, and is associated with malignant status and poor prognosis. The aim of this study is to identify microRNAs (miRNAs), which target DCLK1 to regulate basal-like breast cancer cell proliferation, migration and invasion. In our results, we observed that miR-424-5p expression was decreased in basal-like breast cancer tissues and cell lines. Furthermore, we found 3'-UTR of DCLK1 had binding site of miR-424-5p based on microRNA target databases, and there was an inverse correlation between miR-424-5p and DCLK1 in basal-like breast cancer tissues...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29535532/association-of-doublecortin-like-kinase-1-with-tumor-aggressiveness-and-poor-biochemical-recurrence-free-survival-in-prostate-cancer
#6
Donggen Jiang, Chutian Xiao, Tuzeng Xian, Liantao Wang, Yunhua Mao, Junfu Zhang, Jun Pang
Background: Doublecortin-like kinase 1 (DCLK1) has been proven to be involved in numerous tumors, while its role in prostate cancer (PCa) is still unclear. This study aimed at investigating the expression pattern and prognostic value of DCLK1 in PCa. Patients and methods: Real-time polymerase chain reaction and Western blot were employed to determine DCLK1 mRNA and protein levels in 25 paired fresh samples of PCa and benign prostatic hyperplasia (BPH) as well as in PCa cell lines...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29526803/pancreatic-dclk1-cells-originate-distinctly-from-pdx1-progenitors-and-contribute-to-the-initiation-of-intraductal-papillary-mucinous-neoplasm-in-mice
#7
Wanglong Qiu, Helen E Remotti, Sophia M Tang, Elizabeth Wang, Lily Dobberteen, Ayman Lee Youssof, Joo Hee Lee, Edwin C Cheung, Gloria H Su
PanINs and IPMNs are the two most common precursor lesions that can progress to invasive pancreatic ductal adenocarcinoma (PDA). DCLK1 has been identified as a biomarker of progenitor cells in PDA progressed from PanINs. To explore the potential role of DCLK1-expressing cells in the genesis of IPMNs, we compared the incidence of DCLK1-positive cells in pancreatic tissue samples from genetically-engineered mouse models (GEMMs) for IPMNs, PanINs, and acinar to ductal metaplasia by immunohistochemistry and immunofluorescence...
June 1, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29475922/dclk1-expression-in-colorectal-polyps-increases-with-the-severity-of-dysplasia
#8
Aki Takiyama, Toshiaki Tanaka, Shinsuke Kazama, Hiroshi Nagata, Kazushige Kawai, Keisuke Hata, Kensuke Otani, Takeshi Nishikawa, Kazuhito Sasaki, Manabu Kaneko, Shigenobu Emoto, Koji Murono, Hirotoshi Takiyama, Hiroaki Nozawa
BACKGROUND: The expression of doublecortin-like kinase 1 (DCLK1) has been investigated in cancer; however not in precancerous adenomatous polyps. MATERIALS AND METHODS: Immunohistological expression of DCLK1 was evaluated in various grades of adenomas, cancerous polyps, and hyperplastic polyps in resected human tissue specimens. RESULTS: Ninety-two specimens were positive for DCLK1 and 134 were negative. Cancerous polyps showed a high DCLK1 positivity rate compared to adenomas (68...
March 2018: In Vivo
https://www.readbyqxmd.com/read/29416778/modified-mir-15a-has-therapeutic-potential-for-improving-treatment-of-advanced-stage-colorectal-cancer-through-inhibition-of-bcl2-bmi1-yap1-and-dclk1
#9
Andrew Fesler, Hua Liu, Jingfang Ju
Despite advances in colon cancer treatments, resistance and recurrence remain a significant challenge in treating patients. Novel therapeutic strategies are in urgent need to overcome resistance and improve patient outcomes. MicroRNA based therapeutics have potential to help combat resistance. In this study, we have shown that low miR-15a expression correlates with poor patient prognosis. We have demonstrated the therapeutic potential of miR-15a in colon cancer. miR-15a inhibits several important genes ( BCL2, BMI1, YAP1 and DCLK1 ), decreasing cancer progression and resistance...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29399338/expression-of-doublecortin-and-cam-kinase-like-1-protein-in-serrated-neoplasia-of-the-colorectum
#10
Keiko Morio, Kazuo Yashima, Akihiro Tamoto, Kohei Hosoda, Sohei Yamamoto, Taku Iwamoto, Naoki Ueda, Yuichiro Ikebuchi, Koichiro Kawaguchi, Kenichi Harada, Yoshikazu Murawaki, Hajime Isomoto
The adenoma-carcinoma sequence (ACS) and the serrated pathway are two distinct developmental routes leading to the formation of colorectal carcinoma. Recently, the doublecortin and CaM kinase-like-1 protein (DCLK1) has been reported to serve as an intestinal cancer stem cell marker and has been demonstrated to be overexpressed through the ACS; however, there is a lack of reports on the role of DCLK1 in the serrated pathway. To clarify the correlation between DCLK1 protein expression and clinicopathological characteristics of the serrated tumorigenic pathway, the present study used immunohistochemistry to examine the expression of DCLK1 in endoscopically resected samples of 62 serrated polyps [20 hyperplastic polyps (HPs), 16 traditional serrated adenomas (TSAs) and 26 sessile serrated adenoma-polyps (SSA/Ps)], as well as 20 non-serrated adenomas, 20 carcinoma in adenomas (CIAs) and 18 early pure colorectal carcinomas without any adenoma component (EPCs)...
January 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29344199/expression-and-prognostic-significance-of-doublecortin-like-kinase-1-in-patients-with-hepatocellular-carcinoma
#11
Mengjiao Fan, Niansong Qian, Guanghai Dai
Doublecortin-like kinase 1 (DCLK1), a putative cancer stem cell marker in intestinal and pancreatic tumors, is associated with tumor pathogenesis and progression, and poor survival outcomes in numerous types of cancer. However, DCLK1 expression and its prognostic value remain unclear in hepatocellular carcinoma (HCC). In the present study, the expression of DCLK1 was assessed using immunohistochemistry in 96 resected HCC and 68 adjacent tissue specimens. The staining intensity and the percentage of stained cells were scored on a scale of 0-3 and 0-4, respectively...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29277893/dclk1-promotes-epithelial-mesenchymal-transition-via-the-pi3k-akt-nf-%C3%AE%C2%BAb-pathway-in-colorectal-cancer
#12
Weiying Liu, Shixing Wang, Qi Sun, Zhen Yang, Min Liu, Hua Tang
Double cortin-like kinase 1 (DCLK1) plays important roles during the epithelial-mesenchymal transition (EMT) process in human colorectal cancer (CRC). However, the role of DCLK1 in regulating the EMT of CRC is still poorly understood. In this study, we report evidence that DCLK1 acts as a potent oncogene to drive its extremely malignant character of EMT in an NF-κB-dependent manner in CRC cells. Mechanistic investigations showed that DCLK1 induced the NF-κBp65 subunit expression through the PI3K/Akt/Sp1 axis and activated NF-κBp65 through the PI3K/Akt/IκBα pathway during the EMT of CRC cells...
May 15, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29246000/increased-dclk1-correlates-with-the-malignant-status-and-poor-outcome-in-malignant-tumors-a-meta-analysis
#13
Wenhua Shi, Fangwei Li, Shaojun Li, Jian Wang, Qingting Wang, Xin Yan, Qianqian Zhang, Limin Chai, Manxiang Li
Doublecortin-like kinase 1 (DCLK1) has been found to be involved in malignant biological behavior of cancers and poor prognosis of cancer patients. The aim of this meta-analysis was to systematically clarify the relationships between expression level of DCLK1 and clinicopathological characteristics in tumors and assess its clinical value in cancer diagnosis and prognosis. 18 eligible studies with a total of 2660 patients were identified by searching the electronic bibliographic databases. Pooled results showed that DCLK1 was highly expressed in tissues from cancer patients compared to normal tissues (OR, 10...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29224010/microrna-195-suppresses-the-progression-of-pancreatic-cancer-by-targeting-dclk1
#14
Bin Zhou, Chuandong Sun, Xiao Hu, Hanxiang Zhan, Hao Zou, Yujie Feng, Fabo Qiu, Shun Zhang, Liqun Wu, Bingyuan Zhang
BACKGROUND/AIMS: Doublecortin-like kinase 1 (DCLK1) is emerging as a tumor-specific stem cell marker in pancreatic cancer (PC). MicroRNA-195 (miR-195) plays an important role in many types of tumors. However, the roles of DCLK1 in cancer and miRNAs that directly regulate DCLK1 have not been elucidated. The goal of this study is to assess the effects of miR-195 on inhibiting DCLK1 and to clarify the regulating mechanism of miR-195-DCLK1 in PC cells. METHODS: The expression of DCLK1 protein and miR-195 in PC tissues and adjacent healthy pancreatic tissues was detected by Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR), respectively and the correlation between overall survival of PC patients and expression of DCLK1 was measured by Kaplan-Meier analysis...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29212456/helicobacter-induced-gastric-inflammation-alters-the-properties-of-gastric-tissue-stem-progenitor-cells
#15
Wataru Shibata, Soichiro Sue, Sachiko Tsumura, Yasuaki Ishii, Takeshi Sato, Eri Kameta, Makoto Sugimori, Hiroaki Yamada, Hiroaki Kaneko, Tomohiko Sasaki, Tomohiro Ishii, Toshihide Tamura, Masaaki Kondo, Shin Maeda
BACKGROUND: Although Helicobacter-induced gastric inflammation is the major predisposing factor for gastric carcinogenesis, the precise mechanism by which chronic gastritis causes gastric cancer remains unclear. Intestinal and spasmolytic polypeptide-expressing metaplasia (SPEM) is considered as precancerous lesions, changes in epithelial tissue stem/progenitor cells after chronic inflammation has not been clarified yet. In this study, we utilized three-dimensional gastric epithelial cell culture systems that could form organoids, mimicking gastric epithelial layer, and characterized the changes in epithelial cells after chronic Helicobacter felis infection...
December 6, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/29053464/co-expression-network-approach-to-studying-the-effects-of-botulinum-neurotoxin-a
#16
Kavitha Mukund, Samuel R Ward, Richard L Lieber, Shankar Subramaniam
Botulinum Neurotoxin A (BoNT-A) is a potent neurotoxin with several clinical applications.The goal of this study was to utilize co-expression network theory to analyze temporal transcriptional data from skeletal muscle after BoNT-A treatment. Expression data for 2000 genes (extracted using a ranking heuristic) served as the basis for this analysis. Using weighted gene co-expression network analysis (WGCNA), we identified 19 co-expressed modules, further hierarchically clustered into 5 groups. Quantifying average expression and co-expression patterns across these groups revealed temporal aspects of muscle's response to BoNT-A...
October 16, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/29048622/enhancement-of-cytotoxic-effects-of-gemcitabine-by-dclk1-inhibition-through-suppression-of-chk1-phosphorylation-in-human-pancreatic-cancer-cells
#17
Daichi Kawamura, Yoshihiro Takemoto, Arata Nishimoto, Koji Ueno, Tohru Hosoyama, Bungo Shirasawa, Toshiki Tanaka, Naruji Kugimiya, Eijiro Harada, Kimikazu Hamano
Although gemcitabine (GEM) is frequently used in the treatment of pancreatic cancer, the effects are limited. To increase the inhibitory effect of GEM, the identification of a molecular target is needed. Recent studies have revealed that doublecortin-like kinase 1 (Dclk1) positively regulates tumor growth, invasion, metastasis, factors related to epithelial-mesenchymal transition (EMT), pluripotency, angiogenesis, and anti-apoptosis in pancreatic cancer cells. Therefore, Dclk1 is a potential therapeutic target for pancreatic cancer...
September 20, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28946555/lgr5high-dclk1high-phenotype-is-more-common-in-early-stage-and-intestinal-subtypes-of-gastric-carcinomas
#18
Elham Kalantari, Mohammad Hossein Asadi Lari, Raheleh Roudi, Alireza Korourian, Zahra Madjd
BACKGROUND: Gastric carcinoma is the third most common malignancy and is one of the main causes of cancer deaths worldwide. Cancer stem cells (CSCs) are a subpopulation of tumour cells capable of self-renewal and differentiation, likely responsible for the initiation, recurrence, metastasis and chemo/radio-resistance. OBJECTIVE: This study was conducted to evaluate the expression patterns and clinicopathologic significance of putative CSC markers, Lgr5 and DCLK1, in gastric carcinoma...
December 6, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28887549/satb2-%C3%AE-catenin-tcf-lef-pathway-induces-cellular-transformation-by-generating-cancer-stem-cells-in-colorectal-cancer
#19
Wei Yu, Yiming Ma, Sharmila Shankar, Rakesh K Srivastava
Recent studies have demonstrated the involvement of colorectal cancer (CRC) stem cells (CSC) in transformation, cancer progression and metastasis. The main goal of this paper was to examine the molecular mechanisms by which SATB2 induced malignant transformation of colorectal epithelial cells. SATB2 induced malignant transformation and these transformed cells gained the characteristics of CSCs by expressing stem cell markers (CD44, CD133, LGR5 and DCLK1) and transcription factors (c-Myc, Nanog and Sox2). Overexpression of SATB2 in normal colorectal epithelial cells increased cell motility, migration and invasion, which were associated with an increase in N-cadherin and Zeb1, and decrease in E-cadherin expression...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28883702/doublecortin-and-cam-kinase-like-1-as-an-independent-prognostic-factor-in-patients-with-resected-pancreatic-carcinoma
#20
Kohei Nishio, Kenjiro Kimura, Ryosuke Amano, Bunzo Nakata, Sadaaki Yamazoe, Go Ohira, Kotaro Miura, Naoki Kametani, Hiroaki Tanaka, Kazuya Muguruma, Kosei Hirakawa, Masaichi Ohira
AIM: To elucidate the effect of expression of doublecortin and CaM kinase-like-1 (DCLK1) in patients with pancreatic ductal adenocarcinoma (PDAC). METHODS: Tumor specimens were obtained from 136 patients with pancreatic cancer who had undergone resection without preoperative therapy between January 2000 and December 2013 at the Department of Surgical Oncology, Osaka City University. The resected specimens were analyzed for associations with clinicopathological data, including DCLK1 expression, epithelial mesenchymal transition (EMT) marker expression, and cancer stem cell (CSC) marker expression...
August 21, 2017: World Journal of Gastroenterology: WJG
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