riffle

Membrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ∼1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a "liquid biopsy," while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry of unfractionated plasma. We have developed an enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma...

The recent surge in the discovery of hidden diversity within rheophilic taxa, particularly in West and East Africa, prompted a closer examination of the extent to which the current taxonomy may obscure the diversity of riffle-dwelling suckermouth catfishes in the genus Chiloglanis in southern Africa. Currently, the region comprises eight valid species within this genus. Seven of them have relatively narrow geographic distribution ranges except for C.neumanni , which is considered to be widely distributed, occurring from the Buzi River system in the south, and its northern limit being the eastward draining river systems in Tanzania...

Bioluminescence imaging is a well-established platform for evaluating engineered cell therapies in preclinical studies. However, despite the discovery of new luciferases and substrates, optimal combinations to simultaneously monitor two cell populations remain limited. This makes the functional assessment of cellular therapies cumbersome and expensive, especially in preclinical in vivo models. In this study, we explored the potential of using a green bioluminescence-emitting click beetle luciferase, CBG99, and a red bioluminescence-emitting firefly luciferase mutant, Akaluc, together to simultaneously monitor two cell populations...

In an effort to expedite the publication of articles, AJHP is posting manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time.

BACKGROUND: Cuspidevia Jäch & Boukal, 1995 is a member of the tribe Macronychini which has the following features: antennae short, 6-10 segmented, aedeagus long and cylindrical, paramere very small, slender or lacking. This genus currently comprises only three species. All species are distributed in China. NEW INFORMATION: Cuspideviapilosus sp. nov. is reported from Guangdong, Guangxi and Jiangxi Provinces in China. Habitus and diagnostic features of the new species are illustrated...

Notalgia paresthetica (NP) is a chronic cutaneous neuropathy characterized by localized pruritus and pain, numbness, and/or paresthesia, often linked to degenerative cervicothoracic changes. Treatment options for NP are limited. This case report details a 54-year-old woman with a six-year history of right-sided periscapular pruritus and cervicothoracic discomfort who presented to a chiropractor upon referral with a prior diagnosis of NP. Prior topical treatments yielded minimal relief. Radiographs revealed degenerative spinal changes at C5/6 and C6/7 which correlated with her periscapular symptom distribution...

The synthesis and structural characterization of the first sulfur-containing derivatives of the C,C-biscarborane { ortho -C2 B10 }2 cluster - thiol, thioether, and disulfide - are reported. The biscarboranyl dithiol (1-HS-C2 B10 H10 )2 exhibits an exceedingly long intracluster carbon-carbon bond length of 1.858(3) Å, which is attributed to the extensive interaction between the lone pairs of the thiol groups and the unoccupied molecular orbital of the carborane cluster. The structures of the doubly deprotonated biscarboranyl dithiolate anion (1-S-C2 B10 H10 )2 2- with various counter cations feature an even longer carbon-carbon bond length of 2...

Here we present an original approach to generate 2D high detail riverbed based on a drone photogrammetric survey, and RTK bathymetry measurements for Mera river in the Italian Alps. The aim is to better represent macro-roughness and riverbed structure of the river, also extending it to an ungauged area. Specifically, we apply a step-by-step approach. I) Depth and average slope of the riverbed were calculated from bathymetry data. II) Thus, a trapezoidal channel with constant slope and variable width was defined using the drone images...

Alfaxalone is a commonly used injectable anesthetic in dogs and cats due to its minimal cardiovascular side effects. Data for its use in mice are limited and demonstrate strain- and sex-associated differences in dose-response relationships. We performed a dose-comparison study of alfaxalone-xylazine-buprenorphine (AXB) in Crl: CFW (SW) mice. Subcutaneous injection of 50 mg/kg alfaxalone-10 mg/kg xylazine-0.1 mg/kg buprenorphine HCl consistently achieved a surgical plane of anesthesia (loss of toe pinch) for 48...

Traditionally, researchers have assessed diet selection by comparing consumed versus available taxa. However, taxonomic assignment is probably irrelevant for predators, who likely base their selection on characteristics including prey size, habitat, or behavior. Here, we use an aquatic insectivore, the threatened Pyrenean Desman ( Galemys pyrenaicus ), as a model species to assess whether biological traits help unravel the criteria driving food and habitat preferences. We reanalyzed data from a previous taxonomy-based study of prey selection in two contrasting streams, one with excellent conservation status and the other affected by diversion for hydropower and forestry...

Fish exhibit complex social behaviours that can influence their stress levels and well-being. However, little is known about the link between social interactions and stress in wild fish, especially in running water environments. While many studies have explored the stress axis in fish, most have focused on specific social contexts, leaving gaps in understanding stress responses to social changes. Our study investigated collective behaviour and stress in wild Italian riffle dace (Telestes muticellus) in a controlled experimental setup simulating a natural river system...

We present a genome assembly from an individual female Elmis aenea (a riffle beetle; Arthropoda; Insecta; Coleoptera; Elmidae). The genome sequence is 516.5 megabases in span. Most of the assembly is scaffolded into 9 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 18.06 kilobases in length.

PURPOSE: Access to research programs and increased diversity in research enrollment may be key to improving diverse populations' health and healthcare outcomes. To facilitate research recruitment, a Research Registry ("Registry"), a pre-recruitment database, was developed at an urban tertiary Autism Center ("Autism Center"). In this study, we examined whether disparities in research participation occur in the pre-research recruitment (pre-recruitment) stage. METHODS: We compared demographic factors of patients seen at the Autism Center (but not enrolled in the Registry) vs...

Reservoir formation in a river system changes a lotic environment to more lacustrine conditions with impacts throughout the ecosystem. In this study, a river reach containing typical salmonid riffle/run habitat was flooded to create a large, deep pool from June to September in each of three years. We test the hypothesis that juvenile Atlantic salmon (Salmo salar) with their preference for run/riffle habitats will respond to the transformation to a lentic environment by moving into adjacent lotic environments...

Losses in freshwater fish diversity might produce a loss in important ecological services provided by fishes in particular habitats. An important gap in our understanding of ecosystem services by fishes is the influence of individuals from different size classes, which is predicted based on known ontogenetic shifts in metabolic demand and diet. I used 20 experimental stream mesocosms located at Konza Prairie Biological Station (KPBS), KS, USA, to assess the influence of fish size on ecosystem properties. Mesocosms included two macrohabitats: one riffle upstream from one pool filled with consistent pebble and gravel substrate...

Intercalation is the process of inserting chemical species into the heterointerfaces of two-dimensional (2D) layered materials. While much research has focused on the intercalation of metals and small gas molecules into graphene, the intercalation of larger molecules through the basal plane of graphene remains challenging. In this work, we present a new mechanism for intercalating large molecules through monolayer graphene to form confined oxide materials at the graphene-substrate heterointerface. We investigate the intercalation of phosphorus pentoxide (P2 O5 ) molecules directly from the vapor phase and confirm the formation of confined P2 O5 at the graphene-substrate heterointerface using various techniques...

Despite lotic systems demonstrating high levels of seasonal and spatial variability, most research and biomonitoring practices do not consider seasonality when interpreting results and are typically focused at the meso-scale (combined pool/riffle samples) rather than considering habitat patch dynamics. We therefore sought to determine if the sampling season (spring, summer and autumn) influenced observed macroinvertebrate biodiversity, structure and function at the habitat unit scale (determined by substrate composition), and if this in turn influenced the assessment of fine sediment (sand and silt) pressures...

In addition to quantifying the taxonomic diversity of aquatic communities, understanding the patterns of alpha functional diversity (α-diversity) and exploring changes in functional dissimilarity (β-diversity) can improve our understanding on how ecosystems respond to environmental changes. In this study, we quantified functional alpha (α) and beta (β) diversity of macrophytic assemblages from river sites in Greece and then, examined relationships with water quality parameters and hydromorphological factors...

We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific Orbitrap mass spectrometers, which have long been the gold standard for high-resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high-quality quantitative measurements across a wide dynamic range...

Three new riffle beetles of the genus Zaitzevia Champion, 1923 are described from China, namely Zaitzeviasichuanensis sp. nov. and Zaitzeviafengtongzhaiensis sp. nov. from Sichuan Province, and Zaitzeviayingzuijieensis sp. nov. from Hunan Province. Habitus and diagnostic features of the new species are illustrated. A checklist of all known Chinese Zaitzevia species is given, and a key and distributional map of Zaitzevia species from the Chinese mainland are provided.