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https://www.readbyqxmd.com/read/28423423/invertebrates-fungal-biomass-and-leaf-breakdown-in-pools-and-riffles-of-neotropical-streams
#1
Renato Tavares Martins, Lidimara Souza da Silveira, Marcos Pereira Lopes, Roberto Gama Alves
We evaluated fungal biomass (as ergosterol concentration) and invertebrate colonization during leaf breakdown of Picramnia sellowii Planch. (Picramniaceae) in pools and riffles of three low-order forested streams in southeastern Brazil. We hypothesized that leaf breakdown will be higher in riffles due to the high physical fragmentation and fungal activity. The experiment was carried out during the dry season of 2012, using 108 litter bags, each containing 3 ± 0.05 g of air-dried leaves. After 7, 15, 30, 60, 90, and 120 d of incubation, six litter bags (riffle = 3 and pool = 3) were removed from each stream...
January 1, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28331072/direct-measurement-of-microtubule-attachment-strength-to-yeast-centrosomes
#2
Kimberly K Fong, Krishna K Sarangapani, Erik C Yusko, Michael Riffle, Aida Llauró, Beth Graczyk, Trisha N Davis, Charles L Asbury
Centrosomes, or spindle pole bodies (SPBs) in yeast, are vital mechanical hubs that maintain load-bearing attachments to microtubules during mitotic spindle assembly, spindle positioning, and chromosome segregation. However, the strength of microtubule-centrosome attachments is unknown, and the possibility that mechanical force might regulate centrosome function has scarcely been explored. To uncover how centrosomes sustain and regulate force, we purified SPBs from budding yeast and used laser trapping to manipulate single attached microtubules in vitro Our experiments reveal that SPB-microtubule attachments are extraordinarily strong, rupturing at forces about 4-fold higher than kinetochore attachments under identical loading conditions...
March 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28296675/eosinophilic-esophagitis-and-esophageal-granular-cell-tumor-an-unexpected-association
#3
Mary Ellen Riffle, Alexandros D Polydorides, Jessica Niakan, Mirna Chehade
Esophageal granular cell tumors (GCTs) are rare. Eosinophilic esophagitis (EoE) is an immune-mediated disease characterized by esophageal eosinophilia despite proton pump inhibitor (PPI) therapy. Given that GCTs occur at sites of scarring and inflammation, we sought to determine the prevalence of EoE in patients with esophageal GCTs. Our center's pathology database was searched for GCT specimens from 1995 to 2014. Slides were blindly rereviewed. GCTs were scored for atypical cytological features. Presence and number of eosinophils in the tumor and the surrounding esophageal epithelium and any EoE features were recorded...
May 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28191870/the-ndc80-complex-bridges-two-dam1-complex-rings
#4
Jae Ook Kim, Alex Zelter, Neil T Umbreit, Athena Bollozos, Michael Riffle, Richard Johnson, Michael J MacCoss, Charles L Asbury, Trisha N Davis
Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling and is regulated by Aurora B kinase. We show that the Ndc80 complex can simultaneously bind and bridge across two Dam1 complex rings through a tripartite interaction, each component of which is regulated by Aurora B kinase...
February 13, 2017: ELife
https://www.readbyqxmd.com/read/28183103/description-of-the-mature-larva-of-i-austrolimnius-i-i-nyctelioides-i-germain-1892-and-i-a-elatus-i-hinton-1941-coleoptera-elmidae
#5
Nicolás Martínez Román, Miguel Archangelsky
Mature larvae of two species of the riffle beetle genus Austrolimnius Carter & Zeck, 1929 are described and illustrated for the first time: Austrolimnius nyctelioides (Germain, 1892) and A. elatus Hinton, 1941. A larval diagnosis for the genus is presented and both larvae are compared with each other and with the previously described larva of A. mucubajiensis Gómez & Bello, 2006. Comments on the species' habitats are included.
January 11, 2017: Zootaxa
https://www.readbyqxmd.com/read/28040495/tips-pentacene-loaded-peo-pdlla-core-shell-nanoparticles-have-similar-cellular-uptake-dynamics-in-m1-and-m2-macrophages-and-in-corresponding-in-vivo-microenvironments
#6
Dylan K McDaniel, Ami Jo, Veronica M Ringel-Scaia, Sheryl Coutermarsh-Ott, Daniel E Rothschild, Michael D Powell, Rui Zhang, Timothy E Long, Kenneth J Oestreich, Judy S Riffle, Richey M Davis, Irving C Allen
Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles...
April 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27890476/spatial-and-temporal-dynamics-of-macrophyte-cover-in-a-large-regulated-river
#7
A Tena, D Vericat, L E Gonzalo, R J Batalla
The River Ebro basin is extensively dammed. Dams alter the geomorphological functioning of the river by altering its flow regime (e.g. reducing mean and maximum discharges), increasing bed stability (armouring) and decreasing turbidity (water clarity). These effects, together with an increase in nutrient concentrations and water temperature, have generated optimal conditions for the proliferation of aquatic macrophytes. In this paper, we analyse the temporal and spatial changes of macrophyte cover in the lowermost Ebro through a series of field campaigns carried out between 2009 and 2010...
November 24, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27824341/critical-decisions-in-metaproteomics-achieving-high-confidence-protein-annotations-in-a-sea-of-unknowns
#8
Emma Timmins-Schiffman, Damon H May, Molly Mikan, Michael Riffle, Chris Frazar, H R Harvey, William S Noble, Brook L Nunn
No abstract text is available yet for this article.
February 2017: ISME Journal
https://www.readbyqxmd.com/read/27723440/hairworm-infection-and-seasonal-changes-in-paratenic-hosts-in-a-mountain-stream-in-japan
#9
J Yamashita, T Sato, K Watanabe
For parasites with complex life cycles, the ecological traits determining host competence and seasonal changes in infection in natural habitats are often unclear, making it difficult to predict infection dynamics, including disease outbreaks. Hairworms (phylum Nematomorpha) require both aquatic and terrestrial hosts to complete their life cycle. Although hairworm host competencies have been tested in laboratory experiments, knowledge of the paratenic hosts (aquatic insect larvae) in their natural habitats is limited...
February 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/27672404/de-novo-assembly-and-analysis-of-the-chilean-pencil-catfish-trichomycterus-areolatus-transcriptome
#10
Thomas T Schulze, Jonathan M Ali, Maggie L Bartlett, Madalyn M McFarland, Emalie J Clement, Harim I Won, Austin G Sanford, Elyssa B Monzingo, Matthew C Martens, Ryan M Hemsley, Sidharta Kumar, Nicolas Gouin, Alan S Kolok, Paul H Davis
Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment...
2016: Journal of Genomics
https://www.readbyqxmd.com/read/27644858/remote-actuation-of-magnetic-nanoparticles-for-cancer-cell-selective-treatment-through-cytoskeletal-disruption
#11
Alyssa M Master, Philise N Williams, Nikorn Pothayee, Nipon Pothayee, Rui Zhang, Hemant M Vishwasrao, Yuri I Golovin, Judy S Riffle, Marina Sokolsky, Alexander V Kabanov
Motion of micron and sub-micron size magnetic particles in alternating magnetic fields can activate mechanosensitive cellular functions or physically destruct cancer cells. However, such effects are usually observed with relatively large magnetic particles (>250 nm) that would be difficult if at all possible to deliver to remote sites in the body to treat disease. Here we show a completely new mechanism of selective toxicity of superparamagnetic nanoparticles (SMNP) of 7 to 8 nm in diameter to cancer cells...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27541752/does-type-of-substratum-affects-chironomid-larvae-assemblage-composition-a-study-in-a-river-catchment-in-northern-patagonia-argentina
#12
M Mauad, A Siri, M Donato
Chironomid larvae assemblages were investigated at seven sampling stations in relation to stream habitat type in the Challhuaco-Ñireco river system located in the Nahuel Huapi National Park, in the North-Western part of Rio Negro Province, Argentina. A total of 2229 individuals were sampled and 43 Chironomidae taxa were recorded with Orthocladiinae (29) being the best represented subfamily, followed by Diamesinae (5), Tanypodinae (3), Podonominae (3) and Chironominae (3). Stictocladius spF, Cricotopus (Paratrichocladius) sp6, Cricotopus sp2, Cricotopus sp3 and Parapsectrocladius sp2 were the most abundant taxa...
February 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/27302480/proxl-protein-cross-linking-database-a-platform-for-analysis-visualization-and-sharing-of-protein-cross-linking-mass-spectrometry-data
#13
Michael Riffle, Daniel Jaschob, Alex Zelter, Trisha N Davis
ProXL is a Web application and accompanying database designed for sharing, visualizing, and analyzing bottom-up protein cross-linking mass spectrometry data with an emphasis on structural analysis and quality control. ProXL is designed to be independent of any particular software pipeline. The import process is simplified by the use of the ProXL XML data format, which shields developers of data importers from the relative complexity of the relational database schema. The database and Web interfaces function equally well for any software pipeline and allow data from disparate pipelines to be merged and contrasted...
August 5, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27157654/eosinophilic-esophagitis-diagnosed-in-a-patient-with-common-variable-immunodeficiency
#14
Meng Chen, Huaibin M Ko, Mary E Riffle, Doerthe A Andreae, Charlotte Cunningham-Rundles, Mirna Chehade, Paul J Maglione
No abstract text is available yet for this article.
September 2016: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/26986207/differences-found-in-the-macroinvertebrate-community-composition-in-the-presence-or-absence-of-the-invasive-alien-crayfish-orconectes-hylas
#15
Brandye T Freeland-Riggert, Stefan H Cairns, Barry C Poulton, Christopher M Riggert
Introductions of alien species into aquatic ecosystems have been well documented, including invasions of crayfish species; however, little is known about the effects of these introductions on macroinvertebrate communities. The woodland crayfish (Orconectes hylas (Faxon)) has been introduced into the St. Francis River watershed in southeast Missouri and has displaced populations of native crayfish. The effects of O. hylas on macroinvertebrate community composition were investigated in a fourth-order Ozark stream at two locations, one with the presence of O...
2016: PloS One
https://www.readbyqxmd.com/read/26707499/the-topographic-design-of-river-channels-for-form-process-linkages
#16
Rocko A Brown, Gregory B Pasternack, Tin Lin
Scientists and engineers design river topography for a wide variety of uses, such as experimentation, site remediation, dam mitigation, flood management, and river restoration. A recent advancement has been the notion of topographical design to yield specific fluvial mechanisms in conjunction with natural or environmental flow releases. For example, the flow convergence routing mechanism, whereby shear stress and spatially convergent flow migrate or jump from the topographic high (riffle) to the low point (pool) from low to high discharge, is thought to be a key process able to maintain undular relief in gravel bedded rivers...
April 2016: Environmental Management
https://www.readbyqxmd.com/read/26680264/early-comparison-of-inflammatory-vs-fibrostenotic-phenotype-in-eosinophilic-esophagitis-in-a-multicenter-longitudinal-study
#17
Manish B Singla, Mirna Chehade, Diana Brizuela, Corinne L Maydonovitch, Yen-Ju Chen, Mary Ellen Riffle, Sami R Achem, Fouad J Moawad
OBJECTIVES: Eosinophilic esophagitis (EoE) is a chronic inflammatory condition that causes esophageal remodeling and stricture formation. We compared the clinical course of symptoms, endoscopic findings, histology, and changes in phenotype over time in EoE patients with inflammatory and fibrostenotic phenotypes. METHODS: Data were obtained from EoE patients from three medical centers and followed prospectively. Endoscopic features and histology from index and follow-up endoscopies were recorded...
2015: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/26589313/the-evolution-of-an-integrated-ultrasound-curriculum-iusc-for-medical-students-9-year-experience
#18
Richard A Hoppmann, Victor V Rao, Floyd Bell, Mary Beth Poston, Duncan B Howe, Shaun Riffle, Stephen Harris, Ruth Riley, Carol McMahon, L Britt Wilson, Erika Blanck, Nancy A Richeson, Lynn K Thomas, Celia Hartman, Francis H Neuffer, Brian D Keisler, Kerry M Sims, Matthew D Garber, C Osborne Shuler, Michael Blaivas, Shawn A Chillag, Michael Wagner, Keith Barron, Danielle Davis, James R Wells, Donald J Kenney, Jeffrey W Hall, Paul H Bornemann, David Schrift, Patrick S Hunt, William B Owens, R Stephen Smith, Allison G Jackson, Kelsey Hagon, Steven P Wilson, Stanley D Fowler, James F Catroppo, Ali A Rizvi, Caroline K Powell, Thomas Cook, Eric Brown, Fernando A Navarro, Joshua Thornhill, Judith Burgis, William R Jennings, James B McCallum, James M Nottingham, James Kreiner, Robert Haddad, James R Augustine, Norman W Pedigo, Paul V Catalana
Interest in ultrasound education in medical schools has increased dramatically in recent years as reflected in a marked increase in publications on the topic and growing attendance at international meetings on ultrasound education. In 2006, the University of South Carolina School of Medicine introduced an integrated ultrasound curriculum (iUSC) across all years of medical school. That curriculum has evolved significantly over the 9 years. A review of the curriculum is presented, including curricular content, methods of delivery of the content, student assessment, and program assessment...
December 2015: Critical Ultrasound Journal
https://www.readbyqxmd.com/read/26560693/the-molecular-architecture-of-the-dam1-kinetochore-complex-is-defined-by-cross-linking-based-structural-modelling
#19
Alex Zelter, Massimiliano Bonomi, Jae ook Kim, Neil T Umbreit, Michael R Hoopmann, Richard Johnson, Michael Riffle, Daniel Jaschob, Michael J MacCoss, Robert L Moritz, Trisha N Davis
Accurate segregation of chromosomes during cell division is essential. The Dam1 complex binds kinetochores to microtubules and its oligomerization is required to form strong attachments. It is a key target of Aurora B kinase, which destabilizes erroneous attachments allowing subsequent correction. Understanding the roles and regulation of the Dam1 complex requires structural information. Here we apply cross-linking/mass spectrometry and structural modelling to determine the molecular architecture of the Dam1 complex...
November 12, 2015: Nature Communications
https://www.readbyqxmd.com/read/26531711/response-of-invertebrates-from-the-hyporheic-zone-of-chalk-rivers-to-eutrophication-and-land-use
#20
Octavian Pacioglu, Oana Teodora Moldovan
Whereas the response of lotic benthic macroinvertebrates to different environmental stressors is a widespread practice nowadays in assessing the water and habitat quality, the use of hyporheic zone invertebrates is still in its infancy. In this study, classification and regression trees analysis were employed in order to assess the ecological requirements and the potential as bioindicators for the hyporheic zone invertebrates inhabiting four lowland chalk rivers (south England) with contrasting eutrophication levels (based on surface nitrate concentrations) and magnitude of land use (based on percentage of fine sediments load and median interstitial space)...
March 2016: Environmental Science and Pollution Research International
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