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https://www.readbyqxmd.com/read/28513556/microscale-quantitative-analysis-of-polyhydroxybutyrate-in-prokaryotes-using-idms
#1
Mariana Itzel Velasco Alvarez, Angela Ten Pierick, Patricia T N van Dam, Reza Maleki Seifar, Mark C M van Loosdrecht, S Aljoscha Wahl
Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale and speed of these approaches. Here, we present a method based on isotope dilution mass spectrometry (IDMS) and propanolysis extraction of poly(3-hydroxybutyrate) from an Escherichia coli strain engineered for PHB production...
May 17, 2017: Metabolites
https://www.readbyqxmd.com/read/28474705/-quality-of-life-in-patients-with-psoriasis
#2
Liliana García-Sánchez, Álvaro José Montiel-Jarquín, Eduardo Vázquez-Cruz, Adriana May-Salazar, Itzel Gutiérrez-Gabriel, Jorge Loría-Castellanoso
INTRODUCTION: Psoriasis is a chronic inflammatory skin disease, in which an autoimmune mechanism participates, triggering an accelerated keratopoiesis. Its etiology is unknown; environmental factors, trauma, and infections are involved. The aim of this paper is to present the correlation between the index of severity of psoriasis and quality of life in patients with psoriasis. METHODS: This was a cross-sectional study in 72 patients with psoriasis, older than 15 years old, who agreed to participate in the study...
March 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/28421790/abdominal-obesity-is-strongly-associated-to-blood-pressure-in-young-mexicans
#3
Rene Urquidez Romero, Miguel Murguía Romero, Julián Esparza Romero, Beatriz Araceli Díaz Torres, Alejandra Rodríguez Tadeo, Gabriel Medrano Donlucas, Arnulfo Ramos Jiménez, Abraham Wall Medrano, Itzell A Gallardo Ortíz, Diana C Tapia-Pancardo C Tapia Pancardo, A René Méndez Cruz, J Rafael Jiménez Flores, Rafael Villalobos Molina
OBJECTIVE: The objective of this study was to determine associations between abdominal obesity (AOb) and the other components of metabolic syndrome (MetS) in young Mexicans in a cross-sectional survey completed during a 4 year period. METHODS: This cross-sectional study reports on components and prevalence of MetS by using Alberti et al. (16) criteria, as well as association between AOb and elevated blood pressure (BP) of 2,993 Mexican university students, ages 17 to 25 years (66% women) from central and northern Mexico, over a 4-year survey (2010-2013)...
March 30, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28336214/indoleamine-2-3-dioxygenase-1-ido1-inhibitors-activate-the-aryl-hydrocarbon-receptor
#4
COMPARATIVE STUDY
Benjamin J Moyer, Itzel Y Rojas, Iain A Murray, Seokwon Lee, Haley F Hazlett, Gary H Perdew, Craig R Tomlinson
Indoleamine 2,3-dioxygenase 1 (IDO1) plays a key role in the immune system by regulating tryptophan levels and T cell differentiation. Several tumor types overexpress IDO1 to avoid immune surveillance making IDO1 of interest as a target for therapeutic intervention. As a result, several IDO1 inhibitors are currently being tested in clinical trials for cancer treatment as well as several other diseases. Many of the IDO1 inhibitors in clinical trials naturally bear structural similarities to the IDO1 substrate tryptophan, as such, they fulfill many of the structural and functional criteria as potential AHR ligands...
May 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28331843/curative-effect-of-hf10-on-liver-and-peritoneal-metastasis-mediated-by-host-antitumor-immunity
#5
Yoshihiro Hotta, Hideki Kasuya, Itzel Bustos, Yoshinori Naoe, Toru Ichinose, Maki Tanaka, Yasuhiro Kodera
BACKGROUND: HF10 is a highly attenuated type 1 herpes simplex virus (HSV) with proven effective oncolytic effect. Previous investigations have demonstrated that colon cancer mice model treated with HF10 not only had better survival but were also resistant to the reimplantation of the antitumor effect mediated by host antitumor immunity. Importantly, it has also been noted that in mice with antitumors implanted on both sides of the back, an injection of HF10 on only one side strongly restrains not only the injected antitumor but also the non-injected ones...
2017: Oncolytic Virotherapy
https://www.readbyqxmd.com/read/28285133/modification-of-atgrdp1-gene-expression-affects-silique-and-seed-development-in-arabidopsis-thaliana
#6
Aída Araceli Rodríguez-Hernández, Carlos Vladimir Muro-Medina, Jocelin Itzel Ramírez-Alonso, Juan Francisco Jiménez-Bremont
Glycine Rich Proteins (GRPs) are induced at different developmental stages and in specific plant tissues. Recently, we described a novel Arabidopsis gene encoding a short glycine-rich domain protein (AtGRDP1). This gene is involved in abiotic stress responsiveness; the Atgrdp1-null mutant seeds were more sensitive to stress, while the opposite phenotype was achieved by AtGRDP1 overexpression. In this study, we analyzed the phenotype of the fruits produced by Arabidopsis Atgrdp1 mutants and 35S::AtGRDP1 overexpression lines...
April 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28261992/quality-of-life-in-patients-with-antiphospholipid-syndrome-is-related-to-disease-burden-and-anticoagulant-therapy
#7
Gabriela Hernández-Molina, Itzel González-Pérez, Carlos Pacheco-Molina, Antonio R Cabral
AIM: To evaluate health-related quality of life (HRQoL) in primary antiphospholipid syndrome (PAPS) and correlate it with a crude estimate of accrual organ damage, comorbidity (diabetes mellitus, hypertension and dyslipidemia) and treatment (oral anticoagulation, immunosuppressors and prednisone). METHODS: We assessed HRQoL with the Short-Form 36 (SF-36) and the Lupus Quality of Life instrument (LupusQoL) and the disease burden with a modified Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index (SLICC/ACR SDI)...
March 6, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28204171/the-influence-of-prior-multiport-experience-on-the-learning-curve-for-single-port-thoracoscopic-lobectomy-a-multicentre-comparative-study%C3%A2
#8
Antonio E Martin-Ucar, Javier Aragon, Sergio Bolufer Nadal, Carlos Galvez Munoz, Qigang Luo, Itzel Perez Mendez, Alan D L Sihoe, Laura Socci
OBJECTIVES: Competency in video-assisted thoracoscopic (VATS) lobectomy is estimated to be reached after the surgeon completes 50 cases. We wanted to explore the impact of competency in performing multiport VATS lobectomies on completing the needed number of single-port VATS. METHODS: In a retrospective multicentre study, 6 individual surgeons (3 with previous competency in multiport VATS lobectomy and 3 without) submitted their first 50 cases of single-port VATS lobectomies...
June 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28110881/effect-of-the-continuous-addition-of-ozone-on-biomass-clogging-control-in-a-biofilter-treating-ethyl-acetate-vapors
#9
Itzel Covarrubias-García, Aitor Aizpuru, Sonia Arriaga
Biofiltration systems have been recognized as a cost-effective and environmentally friendly control technique for volatile organic compounds (VOC) removal. However, the long-term operation of biofilters causes biomass accumulation, and thus the occurrence of bed clogging, leading to a major decrease in biofilter performance. Control methods have been carried out in order to solve clogging problems, including backwashing, bed stirring, modification of flow patterns, predation, starvation and others. Ozone (O3) has been used in biofiltration systems at low concentrations to control the excess of biomass...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28099703/prevalence-and-3-year-persistence-of-human-papillomavirus-serotypes-in-asymptomatic-patients-in-northern-mexico
#10
Oscar R Fajardo-Ramírez, María C Barboza-Cerda, Rocío Ortiz-López, Augusto Rojas-Martínez, María L Garza-Rodríguez, Abel Sepúlveda-Flores, Juan F González-Guerrero, Sofía Bernal-Silva, Ricardo M Cerda-Flores, Itzel E Calleja-Macías, Socorro Rodríguez-Flores, Esthela Sandoval-Guzmán, Teresa Plascencia-Solis, Patricia Pérez-Reyes, Jesús Z Villarreal, Hugo A Barrera-Saldaña
OBJECTIVE: To investigate clinical outcomes and 3-year persistence of human papillomavirus (HPV) infections among women in Mexico. METHODS: A prospective study enrolled sexually active women attending primary healthcare clinics in metropolitan Monterrey, Mexico, between June 3 and August 30, 2002. Baseline data were collected and participants underwent HPV screening. Patients with HPV infections were asked to attend a repeat screening appointment after 3 years, when the same screening data were gathered...
January 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/27916816/blueberry-consumption-affects-serum-uric-acid-concentrations-in-older-adults-in-a-sex-specific-manner
#11
Carol L Cheatham, Itzel Vazquez-Vidal, Amanda Medlin, V Saroja Voruganti
Blueberries are rich in antioxidants and may protect against disease. Uric acid accounts for about 50% of the antioxidant properties in humans. Elevated levels of serum uric acid (SUA) or hyperuricemia is a risk factor for cardiovascular disease (CVD). The aim was to determine the effect of blueberries on SUA in older adults. Participants (n = 133, 65-80 years) experiencing mild cognitive impairment (MCI) were randomized in a double-blind 6-month clinical trial to either blueberry or placebo. A reference group with no MCI received no treatment...
November 29, 2016: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/27905541/serbian-language-version-of-the-modified-checklist-for-autism-in-toddlers-revised-with-follow-up-cross-cultural-adaptation-and-assessment-of-reliability
#12
Mia Carakovac, Jelena Jovanovic, Marko Kalanj, Nenad Rudic, Olivera Aleksic-Hil, Branko Aleksic, Itzel Bustos Villalobos, Hideki Kasuya, Norio Ozaki, Dusica Lecic-Tosevski, Milica Pejovic-Milovancevic
Early detection of Autism Spectrum Disorder (ASD) has proven to be of high significance, however there is a limited availability of ASD screening tools in Serbian language. In this study we aim to translate, assess reliability and, in part, test the applicability of Modified Checklist for Autism in Toddlers, Revised, with Follow-Up (M-CHAT R/F) in Serbian Healthcare environment. We screened 128 children in three primary healthcare centres and 20 children in a tertiary psychiatric center, using M-CHAT R/F translated into Serbian language, between December 2014 and October 2015...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894837/cd4-virtual-memory-antigen-inexperienced-t-cells-reside-in-the-na%C3%A3-ve-regulatory-and-memory-t-cell-compartments-at-similar-frequencies-implications-for-autoimmunity
#13
Alina I Marusina, Yoko Ono, Alexander A Merleev, Michiko Shimoda, Hiromi Ogawa, Elizabeth A Wang, Kayo Kondo, Laura Olney, Guillaume Luxardi, Yoshinori Miyamura, Tilahun D Yilma, Itzel Bustos Villalobos, Jennifer W Bergstrom, Daniel G Kronenberg, Athena M Soulika, Iannis E Adamopoulos, Emanual Maverakis
It is widely accepted that central and effector memory CD4(+) T cells originate from naïve T cells after they have encountered their cognate antigen in the setting of appropriate co-stimulation. However, if this were true the diversity of T cell receptor (TCR) sequences within the naïve T cell compartment should be far greater than that of the memory T cell compartment, which is not supported by TCR sequencing data. Here we demonstrate that aged mice with far fewer naïve T cells, respond to the model antigen, hen eggwhite lysozyme (HEL), by utilizing the same TCR sequence as their younger counterparts...
February 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/27818436/hiv-diagnosis-linkage-to-care-and-retention-among-men-who-have-sex-with-men-and-transgender-women-in-guatemala-city
#14
Clare Barrington, Kelly Knudston, Olga Alicia Bailey, Jose Manuel Aguilar, Marilu Itzel Loya-Montiel, Sonia Morales-Miranda
Men who have sex with men (MSM) and transgender women are disproportionately affected by HIV in Guatemala, yet little is known about their experiences with diagnosis, linkage to care, and retention. We conducted qualitative in-depth interviews with 26 MSM and transgender women living with HIV in Guatemala City. HIV diagnosis experiences changed over time with increasing asymptomatic testing at non-governmental organizations. Fear of the physical and social impacts of HIV delayed testing, acceptance of diagnosis, and linkage to HIV care...
2016: Journal of Health Care for the Poor and Underserved
https://www.readbyqxmd.com/read/27799655/balancing-selection-shapes-density-dependent-foraging-behaviour
#15
Joshua S Greene, Maximillian Brown, May Dobosiewicz, Itzel G Ishida, Evan Z Macosko, Xinxing Zhang, Rebecca A Butcher, Devin J Cline, Patrick T McGrath, Cornelia I Bargmann
The optimal foraging strategy in a given environment depends on the number of competing individuals and their behavioural strategies. Little is known about the genes and neural circuits that integrate social information into foraging decisions. Here we show that ascaroside pheromones, small glycolipids that signal population density, suppress exploratory foraging in Caenorhabditis elegans, and that heritable variation in this behaviour generates alternative foraging strategies. We find that natural C. elegans isolates differ in their sensitivity to the potent ascaroside icas#9 (IC-asc-C5)...
November 10, 2016: Nature
https://www.readbyqxmd.com/read/27795219/-hyper-ige-syndrome-lessons-from-function-and-defects-of-stat-3-or-dock-8
#16
Julio César Alcántara-Montiel, Brittany Itzel Vega-Torres
In the classification of primary immunodeficiencies, hyper-IgE syndrome, identified with OMIM code # 147060 in the Online Mendelian Inheritance in Man catalog, belongs to the group of syndromes associated with combined immunodeficiencies. It is characterized by elevated levels of IgE, eosinophilia, recurrent skin abscesses, pneumonia, lung parenchyma lesions, recurrent infections, rashes in newborns, eczema, sinusitis, otitis, and mucocutaneous candidiasis. Hyper-IgE syndrome can be transmitted by autosomal dominant or autosomal recessive modes of inheritance...
October 2016: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/27792702/-diagnosis-of-the-productive-capacity-of-the-imss-regarding-health-technologies
#17
Alejandro Figueroa-Lara, Fátima Itzel López-Fernández, Adriana López-Domínguez, German Fajardo-Dolci
OBJECTIVE: To quantify the production capacity and performance in research and technological developments of the Mexican Social Security Institute (IMSS). MATERIAL AND METHODS: We identified and analyzed information of the legislation, human and financial resources, and infrastructure addressed for research and technological development of IMSS. We analyzed whether the information on the legal framework contained key features to boost research and technological development...
September 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27790940/genetic-variability-of-cyp2c9-2-and-cyp2c9-3-in-seven-indigenous-groups-from-mexico
#18
Katy Sánchez-Pozos, Carolina Rivera-Santiago, María Helena García-Rodríguez, María Guadalupe Ortiz-López, Barbara Itzel Peña-Espinoza, María de Los Ángeles Granados-Silvestre, Adrian Llerena, Marta Menjívar
AIM: CYP2C9 is one of the major drug metabolizing enzymes, however, little is known about polymorphisms in CYP2C9 gene and pharmacological implications in Mexican indigenous populations. Thus, frequencies of CYP2C9*2 and CYP2C9*3 alleles were evaluated in indigenous groups located in northwest (Cora), center (Mazahua and Teenek), south (Chatino and Mixteco) and southeast (Chontal and Maya) regions Mexico. MATERIALS & METHODS: Allelic discrimination was performed by real-time PCR...
October 28, 2016: Pharmacogenomics
https://www.readbyqxmd.com/read/27783611/lactate-in-amniotic-fluid-predictor-of-labor-outcome-in-oxytocin-augmented-primiparas-deliveries
#19
Eva Wiberg-Itzel, Andrea B Pembe, Hans Järnbert-Pettersson, Margareta Norman, Anna-Carin Wihlbäck, Irene Hoesli, Monya Todesco Bernasconi, Elie Azria, Helena Åkerud, Elisabet Darj
BACKGROUND: One of the major complications related to delivery is labor dystocia, or an arrested labor progress. Many dystocic deliveries end vaginally after administration of oxytocin, but a large numbers of women with labor dystocia will undergo a long and unsafe parturition. As a result of the exertion required in labor, the uterus produces lactate. The uterine production of lactate is mirrored by the level of lactate in amniotic fluid (AFL). OBJECTIVES: To evaluate whether the level of AFL, analysed in a sample of amniotic fluid collected vaginally at arrested labor when oxytocin was needed, could predict labor outcome in nulliparous deliveries...
2016: PloS One
https://www.readbyqxmd.com/read/27756222/analysis-and-description-of-the-stages-of-aspergillus-fumigatus-biofilm-formation-using-scanning-electron-microscopy
#20
Alejandra Itzel González-Ramírez, Adrián Ramírez-Granillo, María Gabriela Medina-Canales, Aída Verónica Rodríguez-Tovar, María Angeles Martínez-Rivera
BACKGROUND: Biofilms are a highly structured consortia of microorganisms that adhere to a substrate and are encased within an extracellular matrix (ECM) that is produced by the organisms themselves. Aspergillus fumigatus is a biotechnological fungus that has a medical and phytopathogenic significance, and its biofilm occurs in both natural and artificial environments; therefore, studies on the stages observed in biofilm formation are of great significance due to the limited knowledge that exists on this specific topic and because there are multiple applications that are being carried out...
October 18, 2016: BMC Microbiology
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