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https://www.readbyqxmd.com/read/27905541/serbian-language-version-of-the-modified-checklist-for-autism-in-toddlers-revised-with-follow-up-cross-cultural-adaptation-and-assessment-of-reliability
#1
Mia Carakovac, Jelena Jovanovic, Marko Kalanj, Nenad Rudic, Olivera Aleksic-Hil, Branko Aleksic, Itzel Bustos Villalobos, Hideki Kasuya, Norio Ozaki, Dusica Lecic-Tosevski, Milica Pejovic-Milovancevic
Early detection of Autism Spectrum Disorder (ASD) has proven to be of high significance, however there is a limited availability of ASD screening tools in Serbian language. In this study we aim to translate, assess reliability and, in part, test the applicability of Modified Checklist for Autism in Toddlers, Revised, with Follow-Up (M-CHAT R/F) in Serbian Healthcare environment. We screened 128 children in three primary healthcare centres and 20 children in a tertiary psychiatric center, using M-CHAT R/F translated into Serbian language, between December 2014 and October 2015...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894837/cd4-virtual-memory-antigen-inexperienced-t-cells-reside-in-the-na%C3%A3-ve-regulatory-and-memory-t-cell-compartments-at-similar-frequencies-implications-for-autoimmunity
#2
Alina I Marusina, Yoko Ono, Alexander A Merleev, Michiko Shimoda, Hiromi Ogawa, Elizabeth A Wang, Kayo Kondo, Laura Olney, Guillaume Luxardi, Yoshinori Miyamura, Tilahun D Yilma, Itzel Bustos Villalobos, Jennifer W Bergstrom, Daniel G Kronenberg, Athena M Soulika, Iannis E Adamopoulos, Emanual Maverakis
It is widely accepted that central and effector memory CD4(+) T cells originate from naïve T cells after they have encountered their cognate antigen in the setting of appropriate co-stimulation. However, if this were true the diversity of T cell receptor (TCR) sequences within the naïve T cell compartment should be far greater than that of the memory T cell compartment, which is not supported by TCR sequencing data. Here we demonstrate that aged mice with far fewer naïve T cells, respond to the model antigen, hen eggwhite lysozyme (HEL), by utilizing the same TCR sequence as their younger counterparts...
November 25, 2016: Journal of Autoimmunity
https://www.readbyqxmd.com/read/27818436/hiv-diagnosis-linkage-to-care-and-retention-among-men-who-have-sex-with-men-and-transgender-women-in-guatemala-city
#3
Clare Barrington, Kelly Knudston, Olga Alicia Bailey, Jose Manuel Aguilar, Marilu Itzel Loya-Montiel, Sonia Morales-Miranda
Men who have sex with men (MSM) and transgender women are disproportionately affected by HIV in Guatemala, yet little is known about their experiences with diagnosis, linkage to care, and retention. We conducted qualitative in-depth interviews with 26 MSM and transgender women living with HIV in Guatemala City. HIV diagnosis experiences changed over time with increasing asymptomatic testing at non-governmental organizations. Fear of the physical and social impacts of HIV delayed testing, acceptance of diagnosis, and linkage to HIV care...
2016: Journal of Health Care for the Poor and Underserved
https://www.readbyqxmd.com/read/27799655/balancing-selection-shapes-density-dependent-foraging-behaviour
#4
Joshua S Greene, Maximillian Brown, May Dobosiewicz, Itzel G Ishida, Evan Z Macosko, Xinxing Zhang, Rebecca A Butcher, Devin J Cline, Patrick T McGrath, Cornelia I Bargmann
The optimal foraging strategy in a given environment depends on the number of competing individuals and their behavioural strategies. Little is known about the genes and neural circuits that integrate social information into foraging decisions. Here we show that ascaroside pheromones, small glycolipids that signal population density, suppress exploratory foraging in Caenorhabditis elegans, and that heritable variation in this behaviour generates alternative foraging strategies. We find that natural C. elegans isolates differ in their sensitivity to the potent ascaroside icas#9 (IC-asc-C5)...
October 31, 2016: Nature
https://www.readbyqxmd.com/read/27795219/-hyper-ige-syndrome-lessons-from-function-and-defects-of-stat-3-or-dock-8
#5
Julio César Alcántara-Montiel, Brittany Itzel Vega-Torres
In the classification of primary immunodeficiencies, hyper-IgE syndrome, identified with OMIM code # 147060 in the Online Mendelian Inheritance in Man catalog, belongs to the group of syndromes associated with combined immunodeficiencies. It is characterized by elevated levels of IgE, eosinophilia, recurrent skin abscesses, pneumonia, lung parenchyma lesions, recurrent infections, rashes in newborns, eczema, sinusitis, otitis, and mucocutaneous candidiasis. Hyper-IgE syndrome can be transmitted by autosomal dominant or autosomal recessive modes of inheritance...
October 2016: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/27792702/-diagnosis-of-the-productive-capacity-of-the-imss-regarding-health-technologies
#6
Alejandro Figueroa-Lara, Fátima Itzel López-Fernández, Adriana López-Domínguez, German Fajardo-Dolci
OBJECTIVE: To quantify the production capacity and performance in research and technological developments of the Mexican Social Security Institute (IMSS). MATERIAL AND METHODS: We identified and analyzed information of the legislation, human and financial resources, and infrastructure addressed for research and technological development of IMSS. We analyzed whether the information on the legal framework contained key features to boost research and technological development...
September 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27790940/genetic-variability-of-cyp2c9-2-and-cyp2c9-3-in-seven-indigenous-groups-from-mexico
#7
Katy Sánchez-Pozos, Carolina Rivera-Santiago, María Helena García-Rodríguez, María Guadalupe Ortiz-López, Barbara Itzel Peña-Espinoza, María de Los Ángeles Granados-Silvestre, Adrian Llerena, Marta Menjívar
AIM: CYP2C9 is one of the major drug metabolizing enzymes, however, little is known about polymorphisms in CYP2C9 gene and pharmacological implications in Mexican indigenous populations. Thus, frequencies of CYP2C9*2 and CYP2C9*3 alleles were evaluated in indigenous groups located in northwest (Cora), center (Mazahua and Teenek), south (Chatino and Mixteco) and southeast (Chontal and Maya) regions Mexico. MATERIALS & METHODS: Allelic discrimination was performed by real-time PCR...
October 28, 2016: Pharmacogenomics
https://www.readbyqxmd.com/read/27783611/lactate-in-amniotic-fluid-predictor-of-labor-outcome-in-oxytocin-augmented-primiparas-deliveries
#8
Eva Wiberg-Itzel, Andrea B Pembe, Hans Järnbert-Pettersson, Margareta Norman, Anna-Carin Wihlbäck, Irene Hoesli, Monya Todesco Bernasconi, Elie Azria, Helena Åkerud, Elisabet Darj
BACKGROUND: One of the major complications related to delivery is labor dystocia, or an arrested labor progress. Many dystocic deliveries end vaginally after administration of oxytocin, but a large numbers of women with labor dystocia will undergo a long and unsafe parturition. As a result of the exertion required in labor, the uterus produces lactate. The uterine production of lactate is mirrored by the level of lactate in amniotic fluid (AFL). OBJECTIVES: To evaluate whether the level of AFL, analysed in a sample of amniotic fluid collected vaginally at arrested labor when oxytocin was needed, could predict labor outcome in nulliparous deliveries...
2016: PloS One
https://www.readbyqxmd.com/read/27756222/analysis-and-description-of-the-stages-of-aspergillus-fumigatus-biofilm-formation-using-scanning-electron-microscopy
#9
Alejandra Itzel González-Ramírez, Adrián Ramírez-Granillo, María Gabriela Medina-Canales, Aída Verónica Rodríguez-Tovar, María Angeles Martínez-Rivera
BACKGROUND: Biofilms are a highly structured consortia of microorganisms that adhere to a substrate and are encased within an extracellular matrix (ECM) that is produced by the organisms themselves. Aspergillus fumigatus is a biotechnological fungus that has a medical and phytopathogenic significance, and its biofilm occurs in both natural and artificial environments; therefore, studies on the stages observed in biofilm formation are of great significance due to the limited knowledge that exists on this specific topic and because there are multiple applications that are being carried out...
October 18, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27721373/inhibition-of-trpv1-channels-by-a-naturally-occurring-omega-9-fatty-acid-reduces-pain-and-itch
#10
Sara L Morales-Lázaro, Itzel Llorente, Félix Sierra-Ramírez, Ana E López-Romero, Miguel Ortíz-Rentería, Barbara Serrano-Flores, Sidney A Simon, León D Islas, Tamara Rosenbaum
The transient receptor potential vanilloid 1 (TRPV1) ion channel is mainly found in primary nociceptive afferents whose activity has been linked to pathophysiological conditions including pain, itch and inflammation. Consequently, it is important to identify naturally occurring antagonists of this channel. Here we show that a naturally occurring monounsaturated fatty acid, oleic acid, inhibits TRPV1 activity, and also pain and itch responses in mice by interacting with the vanilloid (capsaicin)-binding pocket and promoting the stabilization of a closed state conformation...
October 10, 2016: Nature Communications
https://www.readbyqxmd.com/read/27699180/altered-macrophage-and-dendritic-cell-response-in-mif-mice-reveals-a-role-of-mif-for-inflammatory-th1-response-in-type-1-diabetes
#11
Yuriko Itzel Sánchez-Zamora, Imelda Juarez-Avelar, Alicia Vazquez-Mendoza, Marcia Hiriart, Miriam Rodriguez-Sosa
Macrophage migration inhibitory factor (Mif) is highly expressed in type 1 diabetes mellitus (T1DM). However, there is limited information about how Mif influences the activation of macrophages (Mφ) and dendritic cells (DC) in T1DM. To address this issue, we induced T1DM by administering multiple low doses of streptozotocin (STZ) to Mif-/- or wild-type (Wt) BALB/c mice. We found that Mif-/- mice treated with STZ (Mif-/-STZ) developed lower levels of hyperglycemia, inflammatory cytokines, and specific pancreatic islet antigen- (PIAg-) IgG and displayed reduced cellular infiltration into the pancreatic islets compared to Wt mice treated with STZ (WtSTZ)...
2016: Journal of Diabetes Research
https://www.readbyqxmd.com/read/27672361/transgenic-gdnf-positively-influences-proliferation-differentiation-maturation-and-survival-of-motor-neurons-produced-from-mouse-embryonic-stem-cells
#12
Daniel Cortés, Yolanda Robledo-Arratia, Ricardo Hernández-Martínez, Itzel Escobedo-Ávila, José Bargas, Iván Velasco
Embryonic stem cells (ESC) are pluripotent and thus can differentiate into every cell type present in the body. Directed differentiation into motor neurons (MNs) has been described for pluripotent cells. Although neurotrophic factors promote neuronal survival, their role in neuronal commitment is elusive. Here, we developed double-transgenic lines of mouse ESC (mESC) that constitutively produce glial cell line-derived neurotrophic factor (GDNF) and also contain a GFP reporter, driven by HB9, which is expressed only by postmitotic MNs...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27610385/anaplasma-marginale-diversity-virulence-and-vaccine-landscape-through-a-genomics-approach
#13
REVIEW
Rosa Estela Quiroz-Castañeda, Itzel Amaro-Estrada, Sergio Darío Rodríguez-Camarillo
In order to understand the genetic diversity of A. marginale, several efforts have been made around the world. This rickettsia affects a significant number of ruminants, causing bovine anaplasmosis, so the interest in its virulence and how it is transmitted have drawn interest not only from a molecular point of view but also, recently, some genomics research have been performed to elucidate genes and proteins with potential as antigens. Unfortunately, so far, we still do not have a recombinant anaplasmosis vaccine...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27538402/the-proinflammatory-cytokines-ifn%C3%AE-tnf%C3%AE-increased-chromogranin-a-positive-neuroendocrine-cells-in-the-colonic-epithelium
#14
José Antonio Hernández-Trejo, Dimelza Lisett Suárez-Pérez, Itzel Zenidel Gutiérrez-Martínez, Omar E Fernandez-Vargas, Carolina Serrano, Aurora Antonia Candelario Martínez, Marco Antonio Meraz Ríos, Alí Francisco Citalán Madrid, Marcela Hernández Ruíz, Elba Reyes-Maldonado, Ricard Valle-Ríos, Jacobo Feintuch-Unger, Michael Schnoor, Nicolás Villegas-Sepúlveda, Oscar Medina Contreras, Porfirio Nava Domínguez
The gastrointestinal (GI) tract is the largest hormone-producing organ in the body due to a specialized cell population called enteroendocrine cells (EECs). The number of EECs increases in the mucosa of inflammatory bowel disease (IBD) patients; however, the mechanisms responsible for these changes remain unknown. Here, we show that the proinflammatory cytokines IFNγ and TNFα or DSS induced colitis increased the number of EECs producing Chromogranin A (CgA) in the colonic mucosa of C57BL/6J mice. CgA-positive cells were non-proliferating cells enriched with inactive PTEN and autophagy markers...
August 18, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27516989/anxiolytic-like-effect-of-ethanolic-extract-of-argemone-mexicana-and-its-alkaloids-in-wistar-rats
#15
Aideé Itzel Arcos-Martínez, Omar David Muñoz-Muñiz, Miguel Ángel Domínguez-Ortiz, Margarita Virginia Saavedra-Vélez, Maribel Vázquez-Hernández, María Gabriela Alcántara-López
OBJECTIVE: Argemone mexicana is a Papaveracea plant; some reports have shown their antibacterial, anti-cancer, sedative and probably anti-anxiety properties. From their aerial parts, flavonoids and alkaloids have been isolated, which are intrinsically related to some actions on the central nervous system. The aim of this study was to evaluate the anxiolytic-like effects of the plant, using its ethanolic extract and alkaloid-enriched extract obtained from fresh leaves. MATERIAL AND METHODS: Phytochemical screening was carried out together with evaluation of antioxidant capacity and the enrichment of alkaloids present in the extract...
July 2016: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/27397028/-anti-estrogenic-and-anti-androgenic-effects-in-wastewater-during-advanced-treatment-comparison-of-three-in-vitro-bioassays
#16
Linda Gehrmann, Helena Bielak, Maximilian Behr, Fabian Itzel, Sven Lyko, Anne Simon, Gotthard Kunze, Elke Dopp, Martin Wagner, Jochen Tuerk
Endocrine-disrupting chemicals are mainly discharged into the environment by wastewater treatment plants (WWTPs) and are known to induce adverse effects in aquatic life. Advanced treatment with ozone successfully removes such organic micropollutants, but an increase of estrogenic effects after the ozonation of hospital wastewater was observed in previous studies. In order to investigate this effect, estrogenic and androgenic as well as anti-estrogenic and anti-androgenic activities were observed during treatment of hospital wastewater using three different effect-based reporter gene bioassays...
July 11, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27389272/draft-genome-sequence-of-candidatus-mycoplasma-haemobos-a-hemotropic-mycoplasma-identified-in-cattle-in-mexico
#17
Fernando Martínez-Ocampo, Sergio D Rodríguez-Camarillo, Itzel Amaro-Estrada, Rosa Estela Quiroz-Castañeda
We present here the draft genome sequence of the first "Candidatus Mycoplasma haemobos" strain found in cattle in Mexico. This hemotropic mycoplasma causes acute and chronic disease in animals. This genome is a starting point for studying the role of this mycoplasma in coinfections and synergistic mechanisms associated with the disease.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27364575/mesorectal-invasion-depth-in-rectal-carcinoma-is-associated-with-low-survival
#18
Leonardo S Lino-Silva, Reynaldo Loaeza-Belmont, Miguel A Gómez Álvarez, Itzel Vela-Sarmiento, José M Aguilar-Romero, Jorge A Domínguez-Rodríguez, Rosa A Salcedo-Hernández, Erika B Ruiz-García, Héctor A Maldonado-Martínez, Ángel Herrera-Gómez
BACKGROUND: Most cases of rectal cancer (RC) in our institution are in pathologic stage T3. They are a heterogeneous group but have been classified in a single-stage category. We performed the present study to validate the prognostic significance of the mesorectal extension depth (MED) in T3 RC measured in millimeters beyond the muscularis propria plane. MATERIALS AND METHODS: We performed a retrospective analysis of 104 patients with T3 RC who had undergone curative surgery after a course of preoperative chemoradiotherapy at a tertiary referral cancer hospital...
June 8, 2016: Clinical Colorectal Cancer
https://www.readbyqxmd.com/read/27318147/comparison-of-gene-expression-patterns-between-mouse-models%C3%A2-of-nonalcoholic-fatty-liver-disease-and-liver-tissues%C3%A2-from-patients
#19
Andreas Teufel, Timo Itzel, Wiebke Erhart, Mario Brosch, Xiao Yu Wang, Yong Ook Kim, Witigo von Schönfels, Alexander Herrmann, Stefan Brückner, Felix Stickel, Jean-François Dufour, Triantafyllos Chavakis, Claus Hellerbrand, Rainer Spang, Thorsten Maass, Thomas Becker, Stefan Schreiber, Clemens Schafmayer, Detlef Schuppan, Jochen Hampe
BACKGROUND & AIMS: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder in industrialized countries. Mouse models of NAFLD have been used in studies of pathogenesis and treatment, and have certain features of the human disease. We performed a systematic transcriptome-wide analysis of liver tissues from patients at different stages of NAFLD progression (ranging from healthy obese individuals to those with steatosis), as well as rodent models of NAFLD, to identify those that most closely resemble human disease progression in terms of gene expression patterns...
September 2016: Gastroenterology
https://www.readbyqxmd.com/read/27297776/glazed-clay-pottery-and-lead-exposure-in-mexico-current-experimental-evidence
#20
Araceli Diaz-Ruiz, Luis Antonio Tristán-López, Karen Itzel Medrano-Gómez, Juan Alejandro Torres-Domínguez, Camilo Ríos, Sergio Montes
OBJECTIVES: Lead exposure remains a significant environmental problem; lead is neurotoxic, especially in developing humans. In Mexico, lead in human blood is still a concern. Historically, much of the lead exposure is attributed to the use of handcrafted clay pottery for cooking, storing and serving food. However, experimental cause-and-effect demonstration is lacking. The present study explores this issue with a prospective experimental approach. METHODS: We used handcrafted clay containers to prepare and store lemonade, which was supplied as drinking water to pregnant rats throughout the gestational period...
June 14, 2016: Nutritional Neuroscience
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