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Rafael Sentandreu, Antonio Caminero, Itzel Rentería, Claudia León-Ramirez, Luis González-de-la-Vara, Eulogio Valentin-Gomez, José Ruiz-Herrera
The walls of both, yeast and mycelial cells of Candida albicans possess a species specific antigen that is recognized by a monoclonal antibody (MAb 3H8). This antigen can be extracted in the form of a very high Mr complex, close or over 106 Da, by treatment, with β-1,3 glucanase, β mercaptoethanol or dithothreitol, or mild alkali, but not by saturated HF in pyridine, suggesting that the complex is bound to wall β-1,3 glucans, and to proteins by disulfide bonds, but not to β-1,6 glucans. Through its sensitivity to trypsin and different deglycosylation procedures, it was concluded that the epitope is associated to a glycoprotein containing N-glycosidic, but not O-glycosidic mannan moieties...
April 10, 2018: FEMS Yeast Research
Nana Wiberg, Tobias Wirenfeldt Klausen, Tobias Tyrberg, Lennart Nordström, Eva Wiberg-Itzel
OBJECTIVE: To correlate the value of lactate in fetal scalp blood at delivery and the outcomes of the offspring at four years of age. METHODS: Cases where scalp blood lactate was taken within sixty minutes before delivery were identified from the randomized trial "Determination of pH or lactate in fetal scalp blood in management of intrapartum fetal distress". Data were grouped according to the generally accepted cutoffs for normality, pre-acidemia, acidemia and concentrations above mean +2 SD during the second stage...
2018: PloS One
Neftali Vazquez, Lilia Sanchez, Rebecca Marks, Eduardo Martinez, Victor Fanniel, Alma Lopez, Andrea Salinas, Itzel Flores, Jesse Hirschmann, Robert Gilkerson, Erin Schuenzel, Robert Dearth, Reginald Halaby, Wendy Innis-Whitehouse, Megan Keniry
BACKGROUND: Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided adaptive immune systems are found in prokaryotes to defend cells from foreign DNA. CRISPR Cas9 systems have been modified and employed as genome editing tools in wide ranging organisms. Here, we provide a detailed protocol to truncate genes in mammalian cells using CRISPR Cas9 editing. We describe custom donor vector construction using Gibson assembly with the commonly utilized pcDNA3 vector as the backbone...
March 14, 2018: BMC Molecular Biology
María Elizbeth Alvarez-Sánchez, Laura Itzel Quintas-Granados, Laura Isabel Vázquez-Carrillo, Jonathan Puente-Rivera, Alma Villalobos-Osnaya, María Dolores Ponce-Regalado, Minerva Camacho-Nuez
Infection with Trichomonas vaginalis produces a malodorous seropurulent vaginal discharge due to several chemicals, including polyamines. The presence of 1,4-diamino-2-butanone (DAB) reduces the amount of intracellular putrescine by 90%, preventing the cotransport of exogenous spermine. DAB-treated parasites present morphological changes, which are restored by adding exogenous putrescine into the culture medium. However, the effect of polyamines over the trichomonad proteomic profile is unknown. In this study, we used a proteomic approach to analyze the polyamine-depletion and restoration effect by exogenous putrescine on T...
March 7, 2018: Parasitology Research
Wendy Itzel Escobedo-Hinojosa, Erika Gomez-Chang, Karina García-Martínez, Raquel Guerrero Alquicira, Alexandre Cardoso-Taketa, Irma Romero
Gastric ulcers are a worldwide health problem and their poor healing is one of the most important causes for their recurrence. We have previously reported the remarkable gastroprotective and anti- Helicobacter pylori activities of the methanolic extract (CpMet) of Cyrtocarpa procera bark. This work investigates, in a murine model, the CpMet gastroprotective mechanism and establishes its preclinical efficacy in the resolution of ethanol-induced gastric ulcers. The results showed that the gastroprotective activity of CpMet is mainly associated with endogenous NO and prostaglandins, followed by sulfhydryl groups and KATP channels...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Annete Itzel Apodaca-Medina, José Israel Torres-Avendaño, José Guadalupe Rendón-Maldonado, Edith Hilario Torres-Montoya, Beatriz Armida Flores-López, Rosa M Del Angel, Jesús Salvador Velarde-Félix, Víctor Manuel Salomón-Soto, Hipólito Castillo-Ureta
Fourteen pools of Aedes aegypti larvae collected within the urban area of Culiacán, Sinaloa, were analyzed by RT-PCR. The results demonstrate, for the first time, the vertical infection of serotype-2 dengue virus (DENV-2) in Sinaloa, Mexico, suggesting that Ae. aegypti acts as a natural reservoir of DENV-2 in this region.
February 27, 2018: Vector Borne and Zoonotic Diseases
Cindy Jazmín Delgado-Galván, Felipe Padilla-Vaca, Fátima Berenice Ramírez Montiel, Ángeles Rangel-Serrano, Itzel Paramo-Pérez, Fernando Anaya Velázquez, B Franco
Entamoeba histolytica genetic organization and genome structure is complex and under intense research. The genome is fully sequenced, and several tools have been developed for the molecular study of this organism. Nevertheless, good protein tracking tags that are easy to measure and image, like the fluorescent proteins are lacking. In this report, we codon-optimized the red fluorescent protein from the coral Discosoma striata (DsRFP) for its use in E. histolytica and demonstrated functionality in vivo. We envision that this protein can be widely used for the development of transcriptional reporter systems and protein-tagging applications...
February 21, 2018: Experimental Parasitology
Itzel Guerrero-Ríos, Alfonso H Ortiz-Ramírez, Piet W N M van Leeuwen, Erika Martin
The asymmetric allylic alkylation of rac-1,3-diphenyl-3-acetoxyprop-1-ene (I) catalysed by palladium and diverse phosphorus containing ligands [(S)-BINAP, (R,R)-Chiraphite and (R,R)-Et-Duphos] in an ionic liquid [HDBU][OAc] was successfully performed, achieving full conversions and up to 96% ee of the (S)-product when (R,R)-Et-Duphos was used as a ligand. The reaction could be performed using an equimolar amount of substrate, malonate and base DBU, in which case the total products sum to the desired alkylated product and the ionic pair [HDBU][OAc]; this system thus produces its own IL solvent as the only co-product...
February 14, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Juan Carlos Camacho-Chab, María Del Refugio Castañeda-Chávez, Manuel Jesús Chan-Bacab, Ruth Noemí Aguila-Ramírez, Itzel Galaviz-Villa, Pascual Bartolo-Pérez, Fabiola Lango-Reynoso, Carolina Tabasco-Novelo, Christine Gaylarde, Benjamín Otto Ortega-Morales
Cadmium is a major heavy metal found in polluted aquatic environments, mainly derived from industrial production processes. We evaluated the biosorption of solubilized Cd 2+ using the extracellular polymeric substances (EPS) produced by Bacillus sp. MC3B-22 and Microbacterium sp. MC3B-10 (Microbactan); these bacteria were originally isolated from intertidal biofilms off the coast of Campeche, Mexico. EPS were incubated with different concentrations of cadmium in ultrapure water. Residual Cd 2+ concentrations were determined by Inductive Coupled Plasma-Optic Emission Spectrometry and the maximum sorption capacity (Qmax) was calculated according to the Langmuir model...
February 11, 2018: International Journal of Environmental Research and Public Health
Itzel Castillejos-García, Velia Ramírez-Amador, Adela Carrillo-García, Alejandro García-Carrancá, Marcela Lizano, Gabriela Anaya-Saavedra
BACKGROUND: Oral high-risk human papillomavirus (HR-HPV) infections are frequent and persistent among HIV-positive population and are associated with an increased risk for head and neck cancer (HNC). In this study, we sought to determine the incidence, persistence, and clearance of HPV-infections in oral and oropharyngeal samples from HIV/AIDS subjects. METHODS: A longitudinal, observational, and analytical study was performed with an ongoing cohort of HIV/AIDS subjects in Mexico City (September 2013-February 2015)...
January 30, 2018: Journal of Oral Pathology & Medicine
Miguel Ortíz-Rentería, Rebeca Juárez-Contreras, Ricardo González-Ramírez, León D Islas, Félix Sierra-Ramírez, Itzel Llorente, Sidney A Simon, Marcia Hiriart, Tamara Rosenbaum, Sara L Morales-Lázaro
The Transient Receptor Potential Vanilloid 1 (TRPV1) ion channel is expressed in nociceptors where, when activated by chemical or thermal stimuli, it functions as an important transducer of painful and itch-related stimuli. Although the interaction of TRPV1 with proteins that regulate its function has been previously explored, their modulation by chaperones has not been elucidated, as is the case for other mammalian TRP channels. Here we show that TRPV1 physically interacts with the Sigma 1 Receptor (Sig-1R), a chaperone that binds progesterone, an antagonist of Sig-1R and an important neurosteroid associated to the modulation of pain...
February 13, 2018: Proceedings of the National Academy of Sciences of the United States of America
Jonadab E Olguín, Itzel Medina-Andrade, Emmanuel Molina, Armando Vázquez, Thalia Pacheco-Fernández, Rafael Saavedra, Carlos Pérez-Plasencia, Yolanda I Chirino, Felipe Vaca-Paniagua, Luis E Arias-Romero, Emma B Gutierrez-Cirlos, Sonia A León-Cabrera, Miriam Rodriguez-Sosa, Luis I Terrazas
Colorectal cancer (CRC) is the second most commonly diagnosed cancer in women and the third in men in North America and Europe. CRC is associated with inflammatory responses in which intestinal pathology is caused by different cell populations including a T cell dysregulation that concludes in an imbalance between activated T (Tact) and regulatory T (Treg) cells. Treg cells are CD4+Foxp3+ cells that actively suppress pathological and physiological immune responses, contributing to the maintenance of immune homeostasis...
2018: Journal of Cancer
Pichardo Macías Luz Adriana, Ramírez Mendiola Blanca Alcira, Contreras García Itzel Jatziri, Zamudio Hernández Sergio Roberto, Chávez Pacheco Juan Luis, Sánchez Huerta Karla Berenice, Mendoza Torreblanca Julieta Griselda
Levetiracetam (LEV) is an anticonvulsant drug with a unique mechanism of action that is not completely understood. However, its activity profile may involve effects on excitatory and/or inhibitory neurotransmission since the primary target of LEV, synaptic vesicle protein 2A, is ubiquitously expressed in all types of synaptic vesicles. Therefore, the objective of the present study was to explore the effect of LEV (300 mg/kg/day for one week, administered via osmotic mini-pumps) on neurotransmitter release and its probable selective effect on extracellular gamma-amino butyric acid (GABA), glutamate (Glu), aspartate (Asp), glutamine (Gln), taurine (Tau) and glycine (Gly) concentrations (using in vivo microdialysis under basal and high-K+ conditions) in the dorsal hippocampus (DH), a region that undergoes major synaptic changes during epilepsy...
February 2018: Epilepsy Research
L Iorizzo, T W Klausen, E Wiberg-Itzel, F Ovin, N Wiberg
OBJECTIVE: Measurement of fetal scalp blood lactate is a supplementary tool to cardiotocography in the case of a non-reassuring tracing. Several hand-held lactate meters have been launched, all with differentials in absolute values. Therefore, the reference intervals must be calculated for each device. The internationally accepted reference interval is based on measurement with Lactate ProTM with recently got out of production. The aim of this study was to propose cutoffs for normality, preacidemia, and acidemia in fetal scalp blood for Lactate ProTM 2 based on the comparison of lactate values measured with Lactate ProTM and Lactate ProTM 2...
January 4, 2018: Journal of Maternal-fetal & Neonatal Medicine
Dirk A Davis, Jose Manuel Aguilar, Cesar Galindo Arandi, Sanny Northbrook, M Itzel Loya-Montiel, Sonia Morales-Miranda, Clare Barrington
Men who have sex with men (MSM) are disproportionately affected by HIV in Guatemala and may benefit from health navigation programs designed to support timely linkage to HIV care. We conducted qualitative in-depth interviews with MSM (n = 19) linked to care within days of their diagnosis with the support of a health navigator. We used narrative analysis and systematic coding to identify themes related to HIV diagnosis and experiences with navigators. Participants experienced strong feelings of fear upon receiving a positive HIV test result...
December 2017: AIDS Education and Prevention: Official Publication of the International Society for AIDS Education
Luis Alfonso Ortíz-Reyes, Lilia Castillo-Martínez, Arianne Itzel Lupián-Angulo, Daniel Dante Yeh, Héctor Isaac Rocha-González, Aurora Elizabeth Serralde-Zúñiga
BACKGROUND: Unintentional underfeeding is common in patients receiving enteral nutrition (EN), and is associated with increased risk of malnutrition complications. Protocols for EN in critically ill patients have been shown to enhance adequacy, resulting in better clinical outcomes; however, outside of intensive care unit (ICU) settings, the influence of a protocol for EN is unknown. OBJECTIVE: To evaluate the efficacy and safety of implementing an EN protocol in a noncritical setting...
January 2018: Journal of the Academy of Nutrition and Dietetics
Itzel Sifuentes-Romero, Boris M Tezak, Sarah L Milton, Jeanette Wyneken
Experimental and field studies of different turtle species suggest that moisture influences embryonic development and sex ratios, wetter substrates tend to produce more males, and drier substrates produce more females. In this study, we used Trachemys scripta elegans to test the effect of moisture on embryonic development and sex ratios. T. s. elegans eggs were incubated under different temperature and moisture regimes. We monitored embryonic development until stage 22 (after sex determination) and, for the first time, we estimated sex ratios using a male-specific transcriptional molecular marker, Sox9...
November 28, 2017: Zoology: Analysis of Complex Systems, ZACS
Itzell E Hernández-Sánchez, Israel Maruri-López, Steffen P Graether, Juan F Jiménez-Bremont
Dehydrins (DHNs) are intrinsically disordered proteins that play central roles in plant abiotic stress responses; however, how they work remains unclear. Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions. To explore the dimerization ability of the Arabidopsis acidic DHNs AtCOR47 and AtERD10, we conducted an in planta DHN binding assay using the Bimolecular Fluorescence Complementation (BiFC) technique. Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10; interestingly, heterodimeric associations also occurred with these DHNs, and these interactions were observed in the cytosol of tobacco cells...
December 6, 2017: Scientific Reports
Oliver Knoop, Fabian Itzel, Jochen Tuerk, Holger V Lutze, Torsten C Schmidt
Ozonation is used as additional wastewater treatment option to remove recalcitrant micropollutants. It also removes the estrogenic activity found in wastewater but not always the anti-estrogenic activity. This can be explained by an incomplete removal of anti-estrogenic micropollutants or by formation of transformation products (TPs) which retain the activity. The present study investigates the degradation of the anti-estrogenic pharmaceutical tamoxifen in pure water, regarding TP formation and related anti-estrogenic effect using Arxula adeninivorans yeast estrogen screen (A-YES)...
May 1, 2018: Science of the Total Environment
Dmitrii Yu Osadchii, Alma I Olivos-Suarez, Anastasiya V Bavykina, Jorge Gascon
Covalent triazine frameworks (CTFs) are porous organic materials promising for applications in catalysis and separation due to their high stability, adjustable porosity, and intrinsic nitrogen functionalities. CTFs are prepared by ionothermal trimerization of aromatic nitriles; however, multiple side reactions also occur under synthesis conditions, and their influence on the material properties is still poorly described. Here we report the systematic characterization of nitrogen in CTFs using X-ray photoelectron spectroscopy...
December 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
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