Read by QxMD icon Read

Collagen Matrix

Xiaozhao Wang, Zun Chen, Beibei Zhou, Xiyue Duan, Wen-Jian Weng, Kui Cheng, Huiming Wang, Jun Lin
Extracellular matrix (ECM) provides a dynamic and complex environment to determine the fate of stem cells. In this work, in vitro cultured cell sheets were treated with paraformaldehyde or ethanol and eventually become ECM. Such ECM was then immobilized on titanium substrates via polydopamine chemistry. Their effects on bone marrow mesenchymal stromal cells (BMSCs) behaviors were investigated. It was found that paraformaldehyde treated ECM coatings (PT-ECM) showed well-maintained microstructure, whereas that of ethanol treated (ET-ECM) were completely changed...
March 22, 2018: ACS Applied Materials & Interfaces
Takuya Hirose, Ippei Suzuki, Naoki Takahashi, Toshiyuki Fukada, Prasarn Tangkawattana, Kazushige Takehana
Ehlers-Danlos syndrome (EDS) is a group of hereditary diseases caused by mutation of extracellular matrix-related genes. Recently, spondylodysplastic EDS-Zip13 (spEDS-Zip13: OMIM 612350) was recognized as a new EDS type. This current study could reveal various morphometric differences of collagenous population in the proper substance of cornea between the wild type and spEDS-Zip13-knockout (Zip13-KO) mice. Blockade of Smad-signaling pathway might initiate these alterations. Predilected dissimilarity in level of transcriptional activity probably dictated morphology of keratocyte and shape and electron density of its nucleus...
March 22, 2018: Journal of Veterinary Medical Science
Souta Motoike, Mikihito Kajiya, Nao Komatsu, Manabu Takewaki, Susumu Horikoshi, Shinji Matsuda, Kazuhisa Ouhara, Tomoyuki Iwata, Katsuhiro Takeda, Tsuyoshi Fujita, Hidemi Kurihara
BACKGROUND: Three-dimensional (3D) cultured clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. C-MSCs can regulate cellular functions in vitro and can be grafted into a defect site without an artificial scaffold to induce bone regeneration. Long-term cryopreservation of C-MSCs, which can enable them to serve as a ready-to-use cell preparation, may be helpful in developing beneficial cell therapy for bone regeneration...
March 21, 2018: Stem Cell Research & Therapy
Kena Song, Zirui Wang, Ruchuan Liu, Guo Chen, Liyu Liu
Exploring the complicated development of tumors and metastases needs a deep understanding of the physical and biological interactions between cancer cells and their surrounding microenvironments. One of the major challenges is the ability to mimic the complex 3-D tissue microenvironment that particularly influences cell proliferation, migration, invasion, and apoptosis in relation to the extracellular matrix (ECM). Traditional cell culture is unable to create 3-D cell scaffolds resembling tissue complexity and functions, and, in the past, many efforts were made to realize the goal of obtaining cell clusters in hydrogels...
March 21, 2018: International Journal of Molecular Sciences
Yang Wei, Yu-Han Chang, Chung-Jui Liu, Ren-Jei Chung
The susceptibility of guided bone regeneration (GBR) material to infection by pathogens at wound sites during bone healing has often been overlooked. The objective of this study was the synthesis and characterization of a potential material for antibacterial GBR application. In the current study, the mechanical strength and biocompatibility of a composite restoration material-made of oxidized hyaluronic acid (HA)/type I collagen hydrogel integrated with tricalcium phosphate (β-TCP) using a natural crosslinking agent, oligomeric proanthocyanidins (OPCs)-were evaluated...
March 21, 2018: Pharmaceutics
Julie Kohn, Francois Bordeleau, Joseph Miller, Hannah Watkins, Shweta Modi, Jenny Ma, Julian Azar, David Putnam, Cynthia Reinhart-King
Aerobic exercise helps to maintain cardiovascular health in part by mitigating age-induced arterial stiffening. However, the long-term effects of exercise regimens on aortic stiffness remain unknown, especially in the intimal extracellular matrix layer known as the subendothelial matrix. To examine how the stiffness of the subendothelial matrix changes following exercise cessation, mice were exposed to an 8-week swimming regimen followed by an 8-week sedentary rest period. Whole vessel and subendothelial matrix stiffness were measured after both the exercise and rest periods...
March 12, 2018: Journal of Biomechanical Engineering
Massoud Saghafi, Mandana Khodashahi, Nayyereh Saadati, Azita Azarian, Zahra Rezaieyazdi, Maryam Salehi, Maryam Sahebari
Background: Serum cartilage oligomeric matrix protein (COMP) is a non-collagen glycoprotein produced by the cartilage, synovium, tendon, and meniscus. Recent studies showed that COMP is a reliable factor for monitoring cartilage damage. Objective: To determine the relationship between serum COMP concentration and the severity of rheumatoid arthritis (RA). Methods: This cross-sectional study lasted from 2013 to 2015 at the Rheumatology Clinic of Ghaem Hospital, Mashhad, Iran...
December 2017: Electronic Physician
Chien-Liang Fang, Yiwei Wang, Kevin H-Y Tsai, Hsin-I Chang
The dermis of human skin contains large numbers of fibroblasts that are responsible for the production of the extracellular matrix (ECM) that supporting skin integrity, elasticity and wound healing. Previously, an in vivo study demonstrated that dermal fibroblasts siting in the lower dermis are capable to convert into skin adipose layer and hence fibroblast lipogenesis may vary the structure and elasticity of dermis. In the present study, Hs68 human dermal fibroblasts were utilized as an in vitro model to study the lipogenesis via using adipogenic differentiation medium (ADM)...
2018: Frontiers in Pharmacology
Yunus E Demiray, Kati Rehberg, Stefanie Kliche, Oliver Stork
The serine/threonine kinase Ndr2 has been shown to control the inside-out activation of the β1 subunit of integrins and the formation of neurites in both primary neurons and neurally differentiated pheochromacytoma (PC12) cells. In this study, we demonstrate that Ndr2 kinase furthermore determines the substrate specificity of neurite extension in PC12 cells via expression of α1 β1 integrins. We show that stable overexpression of Ndr2 in PC12 cells increases neurite growth and extension on poly-D-lysine substrate, likely involving an increased expression of active β1 integrin in the growth tips of these cells...
2018: Frontiers in Molecular Neuroscience
Michael F Sweeney, Carlos Sonnenschein, Ana M Soto
Background: Three-dimensional cultures of mammary epithelial cells allow for biologically-relevant studies of the development of the mammary gland in rodents and humans under normal and pathological conditions, like carcinogenesis. Under these conditions, mammotropic hormones play significant roles in tissue morphogenesis. Therefore, a system that recreates the normal, hormonally responsive epithelium would be a valuable tool to study the normal state and its transition to carcinogenesis...
2018: Cancer Cell International
Ji-Hyun Lee, Barun Poudel, Hyeon-Hui Ki, Sarmila Nepali, Young-Mi Lee, Jeon-Soo Shin, Dae-Ki Kim
C1q is known to perform several functions in addition to the role it plays in complement activation. C1q contains a collagen-like portion and DDR1 (discoidin domain receptor 1) is a well-known collagen receptor. Accordingly, we hypothesized C1q might be a novel ligand of DDR1. This study shows for the first time C1q directly induces the activation and upregulation of DDR1, and that this leads to enhanced migration and invasion of HepG2 cells. In addition, C1q was found to induce the activations of mitogen-activated protein kinases (MAPKs) and phosphoinositide 3-kinase (PI3K)/Akt signaling, and to increase the expressions of matrix metalloproteinases (MMP2 and 9)...
March 20, 2018: Scientific Reports
Arash Mehdizadeh, Bruce S Gardiner, Michael Lavagnino, David W Smith
The composition of extracellular matrix (ECM) in tendon depends on the secretion profile of resident cells known as tenocytes. For tissues with a mechanical role like tendon, mechanical strain is known to play an important role in determining the secretion profile of resident cells. Previously we explored the idea of estimating average concentrations of ECM molecules as a function of tendon strain magnitude and number of loading cycles. Specifically, we developed a model of the mechanical fatigue damage of tendon collagen fibers and introduced elementary cell responses (ECRs) by which local cellular-level responses to the strain environment, combined with the fatigue damage model, were scaled up to predict tissue-level responses...
March 20, 2018: Connective Tissue Research
Feng Fu, Xiang Zhu, Zhe Qin, Jing-Jing Wang, Chao Xu, Li-Na Wang, Yue Tu, Sai Zhang, Rui-Xin Li, Xiao-Hong Li, Ming-Liang Zhao
Degradation rate is an important index for evaluating biomaterials. The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation. The collagen-chitosan (CG-CS) composite scaffolds were prepared using freeze-drying technology. The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro. The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds...
March 19, 2018: Journal of Materials Science. Materials in Medicine
Cem Gabay, Jérôme Msihid, Moshe Zilberstein, Caroline Paccard, Yong Lin, Neil M H Graham, Anita Boyapati
Introduction: Interleukin-6 (IL-6) orchestrates formation of an inflammatory pannus, leading to joint damage in rheumatoid arthritis (RA). Sarilumab is a human monoclonal antibody blocking the IL-6Rα. In TARGET (NCT01709578), a phase 3 study in adults with moderate-to-severe RA and inadequate response or intolerance to tumour necrosis factor inhibitors, subcutaneous sarilumab 200 mg or 150 mg every 2 weeks (q2w) plus conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) significantly reduced disease activity versus placebo plus csDMARDs...
2018: RMD Open
Hwi Don Lee, Jun Geun Shin, Hoon Hyun, Bong-Ahn Yu, Tae Joong Eom
Tendons are tough, flexible, and ubiquitous tissues that connect muscle to bone. Tendon injuries are a common musculoskeletal injury, which affect 7% of all patients and are involved in up to 50% of sports-related injuries in the United States. Various imaging modalities are used to evaluate tendons, and both magnetic resonance imaging and sonography are used clinically to evaluate tendons with non-invasive and non-ionizing radiation. However, these modalities cannot provide 3-dimensional (3D) structural images and are limited by angle dependency...
March 19, 2018: Scientific Reports
Xingli Ji, Honglian Wang, Zhaojun Wu, Xia Zhong, Menglian Zhu, Yuwei Zhang, Ruizhi Tan, Yuhang Liu, Jianchun Li, Li Wang
BACKGROUND Fibrosis is the common pathological feature in most kinds of chronic kidney disease (CKD). TGF-β/Smads signaling is the master pathway regulating kidney fibrosis pathogenesis, in which Smad3 acts as the integrator of various pro-fibrosis signals. In this study, we analyzed the role of SIS3, a specific inhibitor of Smad3, in mouse unilateral ureteral obstruction (UUO) kidneys. MATERIAL AND METHODS UUO mice were intraperitoneally injected with 0.2 mg/kg/day or 2 mg/kg/day of SIS3 or control saline for 7 days, followed by analysis of structure injury, fibrosis status, inflammation, apoptosis, and TGF-β/Smads signaling activity...
March 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Xiaopeng Zhou, Jingkai Wang, Weijing Fang, Yiqing Tao, Tengfei Zhao, Kaishun Xia, Chengzhen Liang, Jianming Hua, Fangcai Li, Qixin Chen
Nucleus pulposus (NP) degeneration is usually the origin of intervertebral disc degeneration and consequent lower back pain. Although adipose-derived stem cell (ADSC)-based therapy is regarded to be promising for the treatment of degenerated NP, there is a lack of viable cell carriers to transplant ADSCs into the NP while maintaining cell function. In this study, we developed a type II collagen/chondroitin sulfate (CS) composite hydrogel-like ADSC (CCSA) delivery system with genipin as the cross-linking agent...
March 16, 2018: Acta Biomaterialia
Qing Li, Chao Wang, Biao Han, Feini Qu, Hao Qi, Christopher Y Li, Robert L Mauck, Lin Han
To elucidate how maturation impacts the structure and mechanics of meniscus extracellular matrix (ECM) at the length scale of collagen fibrils and fibers, we tested the micromechanical properties of fetal and adult bovine menisci via atomic force microscopy (AFM)-nanoindentation. For circumferential fibers, we detected significant increase in the effective indentation modulus, Eind , with age. Such impact is in agreement with the increase in collagen fibril diameter and alignment during maturation, and is more pronounced in the outer zone, where collagen fibrils are more aligned and packed...
March 9, 2018: Journal of Biomechanics
Adriana Correa Melo, Isabella Cattani-Cavalieri, Marina Valente Barroso, Nicolas Quesnot, Lycia Brito Gitirana, Manuella Lanzetti, Samuel Santos Valença
Emphysema results in a proteinase - antiproteinase imbalance, inflammation and oxidative stress. Our objective was to investigate whether atorvastatin could repair mouse lungs after elastase-induced emphysema. Vehicle (50 μL) or porcine pancreatic elastase (PPE) was administered on day 1, 3, 5 and 7 at 0.6 U intranasally. Male mice were divided into a control group (sham), PPE 32d (sacrificed 24 h after 32 days), PPE 64d (sacrificed 24 h after 64 days), and atorvastatin 1, 5 and 20 mg treated from day 33 until day 64 and sacrificed 24 h later (A1 mg, A5 mg and A20 mg, respectively)...
March 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Lisa A Sawicki, Leila H Choe, Katherine L Wiley, Kelvin H Lee, April M Kloxin
Cells interact with and remodel their microenvironment, degrading large extracellular matrix (ECM) proteins (e.g., fibronectin, collagens) and secreting new ECM proteins and small soluble factors (e.g., growth factors, cytokines). Synthetic mimics of the ECM have been developed as controlled cell culture platforms for use in both fundamental and applied studies. However, how cells broadly remodel these initially well-defined matrices remains poorly understood and difficult to probe. In this work, we have established methods for widely examining both large and small proteins that are secreted by cells within synthetic matrices...
March 12, 2018: ACS Biomaterials Science & Engineering
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"