keyword
MENU ▼
Read by QxMD icon Read
search

Mtap

keyword
https://www.readbyqxmd.com/read/28213009/immunohistochemical-detection-of-mtap-and-bap1-protein-loss-for-mesothelioma-diagnosis-comparison-with-9p21-fish-and-bap1-immunohistochemistry
#1
Tomoyuki Hida, Makoto Hamasaki, Shinji Matsumoto, Ayuko Sato, Tohru Tsujimura, Kunimitsu Kawahara, Akinori Iwasaki, Tatsuro Okamoto, Yoshinao Oda, Hiroshi Honda, Kazuki Nabeshima
OBJECTIVES: Differentiating malignant pleural mesothelioma (MPM) from reactive mesothelial hyperplasia (RMH) is still challenging. Detection of homozygous deletion (HD) of 9p21 region including p16(INK4A) (p16) by fluorescence in situ hybridization (FISH) and immunohistochemical detection of loss of BRCA1 associated protein 1 (BAP1), are reliable markers for MPM diagnosis. However, not all laboratories are equipped to perform 9p21 FISH; immunohistochemistry (IHC) is a more common and feasible technique...
February 2017: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/28026167/heat-capacity-changes-for-transition-state-analogue-binding-and-catalysis-with-human-5-methylthioadenosine-phosphorylase
#2
Ross S Firestone, Scott A Cameron, Jerome M Karp, Vickery L Arcus, Vern L Schramm
Human 5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolysis of 5'-methylthioadenosine (MTA). Its action regulates cellular MTA and links polyamine synthesis to S-adenosylmethionine (AdoMet) salvage. Transition state analogues with picomolar dissociation constants bind to MTAP in an entropically driven process at physiological temperatures, suggesting increased hydrophobic character or dynamic structure for the complexes. Inhibitor binding exhibits a negative heat capacity change (-ΔCp), and thus the changes in enthalpy and entropy upon binding are strongly temperature-dependent...
December 27, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27994653/association-of-common-variants-in-mtap-with-susceptibility-and-overall-survival-of-osteosarcoma-a-two-stage-population-based-study-in-han-chinese
#3
Liqiang Zhi, Dan Liu, Stephen G Wu, Tianqing Li, Guanghui Zhao, Bo Zhao, Meng Li
Osteosarcoma (OS) is a common malignant tumor, which exists widely in the bone of children and adolescents, and genetic factors may influence its susceptibility. Recently, the gene MTAP has been reported to be associated with OS in a Caucasian population. To investigate the association of common variants in MTAP with OS risk in Han Chinese individuals, we designed a two-stage case-control study with 392 OS patients and 1,578 unrelated healthy controls of Han Chinese individuals. A total of 17 tagging single nucleotide polymorphisms (SNPs) were firstly genotyped in the discovery stage, and single-SNP association and haplotypic association analyses have been performed...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/27960044/genetic-variants-at-9p21-3-are-associated-with-risk-of-esophageal-squamous-cell-carcinoma-in-a-chinese-population
#4
Xiaoming Lin, Caiwang Yan, Yong Gao, Jiangbo Du, Xun Zhu, Fei Yu, Tongtong Huang, Juncheng Dai, Hongxia Ma, Yue Jiang, Rong Yin, Zhibin Hu, Guangfu Jin, Lin Xu, Hongbing Shen
Genome-wide association studies (GWAS) have linked genetic variants at 9p21.3 to the risk of multiple cancers. However, the roles of genetic variants at 9p21.3 in esophageal squamous cell carcinoma (ESCC) development are largely unknown. Here we evaluated the genetic variants at 9p21.3 reported in cancer GWAS with case-control study including 2,139 ESCC cases and 2,273 controls in a Chinese population, and measured the mRNA expression levels of MTAP, CDKN2A, CDKN2B and CDKN2B-AS1 in paired ESCC tumor and adjacent normal tissues...
December 13, 2016: Cancer Science
https://www.readbyqxmd.com/read/27935959/crystal-structure-of-schistosoma-mansoni-adenosine-phosphorylase-5-methylthioadenosine-phosphorylase-and-its-importance-on-adenosine-salvage-pathway
#5
Juliana Roberta Torini, José Brandão-Neto, Ricardo DeMarco, Humberto D'Muniz Pereira
Schistosoma mansoni do not have de novo purine pathways and rely on purine salvage for their purine supply. It has been demonstrated that, unlike humans, the S. mansoni is able to produce adenine directly from adenosine, although the enzyme responsible for this activity was unknown. In the present work we show that S. mansoni 5´-deoxy-5´-methylthioadenosine phosphorylase (MTAP, E.C. 2.4.2.28) is capable of use adenosine as a substrate to the production of adenine. Through kinetics assays, we show that the Schistosoma mansoni MTAP (SmMTAP), unlike the mammalian MTAP, uses adenosine substrate with the same efficiency as MTA phosphorolysis, which suggests that this enzyme is part of the purine pathway salvage in S...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27929028/concordance-between-somatic-copy-number-loss-and-down-regulated-expression-a-pan-cancer-study-of-cancer-predisposition-genes
#6
Ran Wei, Ming Zhao, Chun-Hou Zheng, Min Zhao, Junfeng Xia
Cancer predisposition genes (CPGs) are a class of cancer genes in which germline variants lead to increased risk of cancer. Research has revealed that copy number variation (CNV) may be linked to cancer susceptibility in CPGs. In this pan-cancer analysis, we explored the relationship between somatic CNV and gene expression changes in CPGs. Based on curated 827 human CPGs from literature, we firstly identified 729 CPGs with precise CNV information from 5067 tumor samples using TCGA CNV data. Among them, 128 CPGs tended to have more frequent copy number losses (CNLs) compared with copy number gains (CNGs)...
December 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27851804/experimentally-assessed-reactive-aggression-in-borderline-personality-disorder
#7
Olga Kogan-Goloborodko, Elisabeth Brügmann, Jonathan Repple, Ute Habel, Benjamin Clemens
Approximately 73% of patients suffering from Borderline personality disorder (BPD) exhibit aggressive behaviour, which severely hinders therapeutic work and clinical improvement. Because the underlying mechanisms of aggression in BPD are not yet completely understood, additional research in this domain has a high clinical and scientific relevance. We employed a modified version of the Taylor Aggression Paradigm (mTAP), in order to examine for the first time whether this task can be used to differentiate between BPD patients and healthy controls with regard to reactive aggression...
2016: PloS One
https://www.readbyqxmd.com/read/27779859/continuous-fluorescence-assays-for-reactions-involving-adenine
#8
Ross S Firestone, Scott A Cameron, Peter C Tyler, Rodrigo G Ducati, Adam Z Spitz, Vern L Schramm
5'-Methylthioadenosine phosphorylase (MTAP) and 5'-methylthioadenosine nucleosidase (MTAN) catalyze the phosphorolysis and hydrolysis of 5'-methylthioadenosine (MTA), respectively. Both enzymes have low KM values for their substrates. Kinetic assays for these enzymes are challenging, as the ultraviolet absorbance spectra for reactant MTA and product adenine are similar. We report a new assay using 2-amino-5'-methylthioadenosine (2AMTA) as an alternative substrate for MTAP and MTAN enzymes. Hydrolysis or phosphorolysis of 2AMTA forms 2,6-diaminopurine, a fluorescent and easily quantitated product...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27775850/clinicopathological-characteristics-and-genomic-profile-of-primary-sinonasal-tract-diffuse-large-b-cell-lymphoma-dlbcl-reveals-gain-at-1q31-and-rgs1-encoding-protein-high-rgs1-immunohistochemical-expression-associates-with-poor-overall-survival-in-dlbcl-not
#9
Joaquim Carreras, Yara Y Kikuti, Sílvia Beà, Masashi Miyaoka, Shinichiro Hiraiwa, Haruka Ikoma, Ryoko Nagao, Sakura Tomita, David Martin-Garcia, Itziar Salaverria, Ai Sato, Akifumi Ichiki, Giovanna Roncador, Juan F Garcia, Kiyoshi Ando, Elias Campo, Naoya Nakamura
AIMS: We aimed to define the clinicopathological characteristics of 29 primary sinonasal diffuse large B cell lymphoma (DLBCL(sn) ) in a series of 240 cases of DLBCL not otherwise specified [DLBCL(all ()(NOS)()) ], including DLBCL(sn) training set (n = 11) and validation set (n = 18), and DLBCL(non-sn) (n = 211). METHODS AND RESULTS: In the training set, 82% had a non-germinal center B-cell-like (Hans' Classifier) (non-GCB) phenotype and 18% were Epstein-Barr virus-encoded small RNAs (EBER)(+) ...
March 2017: Histopathology
https://www.readbyqxmd.com/read/27761950/melanoma-risk-alleles-are-associated-with-down-regulation-of-the-mtap-gene-and-hyper-methylation-of-a-cpg-island-upstream-of-the-gene-in-dermal-fibroblasts
#10
A Sangalli, G Malerba, G Tessari, M Rodolfo, M Gomez-Lira
No abstract text is available yet for this article.
October 20, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27622058/suppressive-effects-of-tumor-cell-derived-5-deoxy-5-methylthioadenosine-on-human-t-cells
#11
Frederik C Henrich, Katrin Singer, Kerstin Poller, Luise Bernhardt, Carolin D Strobl, Katharina Limm, Axel P Ritter, Eva Gottfried, Simon Völkl, Benedikt Jacobs, Katrin Peter, Dimitrios Mougiakakos, Katja Dettmer, Peter J Oefner, Anja-Katrin Bosserhoff, Marina P Kreutz, Michael Aigner, Andreas Mackensen
The immunosuppressive tumor microenvironment represents one of the main obstacles for immunotherapy of cancer. The tumor milieu is among others shaped by tumor metabolites such as 5'-deoxy-5'-methylthioadenosine (MTA). Increased intratumoral MTA levels result from a lack of the MTA-catabolizing enzyme methylthioadenosine phosphorylase (MTAP) in tumor cells and are found in various tumor entities. Here, we demonstrate that MTA suppresses proliferation, activation, differentiation, and effector function of antigen-specific T cells without eliciting cell death...
August 2016: Oncoimmunology
https://www.readbyqxmd.com/read/27556634/concordance-of-copy-number-loss-and-down-regulation-of-tumor-suppressor-genes-a-pan-cancer-study
#12
Min Zhao, Zhongming Zhao
BACKGROUND: Tumor suppressor genes (TSGs) encode the guardian molecules to control cell growth. The genomic alteration of TSGs may cause tumorigenesis and promote cancer progression. So far, investigators have mainly studied the functional effects of somatic single nucleotide variants in TSGs. Copy number variation (CNV) is another important form of genetic variation, and is often involved in cancer biology and drug treatment, but studies of CNV in TSGs are less represented in literature...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27491082/histologic-characterization-of-regenerated-tissues-after-pulp-revascularization-of-immature-dog-teeth-with-apical-periodontitis-using-tri-antibiotic-paste-and-platelet-rich-plasma
#13
Carlos Stambolsky, Soledad Rodríguez-Benítez, José Luis Gutiérrez-Pérez, Daniel Torres-Lagares, Jenifer Martín-González, Juan José Segura-Egea
INTRODUCTION: This study evaluates histologically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP). METHODS: Forty double-rooted immature premolar teeth from 4 female Beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP. Four teeth were left untreated and assigned to the positive control group. The last 28 teeth were randomly assigned into four experimental groups of 8 teeth, each one treated with a different treatment protocol: A1, sodium hypochlorite (SH)+blood clot (BC); A2, SH+platelet-rich plasma (PRP); B1, SH+modified tri-antibiotic paste (mTAP)+BC; B2, SH+mTAP+PRP...
November 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27479139/characterization-of-the-methylthioadenosine-phosphorylase-polymorphism-rs7023954-incidence-and-effects-on-enzymatic-function-in-malignant-melanoma
#14
Katharina Limm, Katja Dettmer, Jörg Reinders, Peter J Oefner, Anja-Katrin Bosserhoff
Deficiency of methylthioadenosine phosphorylase (MTAP) supports melanoma development and progression through accumulation of its substrate 5'-methylthioadenosine (MTA), which leads amongst others to a constitutive inhibition of protein arginine methyltransferases (PRMTs) and activation of the transcription factor AP-1 via the receptor ADORA2B. Genetic association studies have also suggested that genetic polymorphism in MTAP may modulate the risk of melanoma. Here, we investigated the only globally common non-synonymous single nucleotide polymorphism (SNP) reported to date for MTAP...
2016: PloS One
https://www.readbyqxmd.com/read/27433286/the-dysregulation-of-polyamine-metabolism-in-colorectal-cancer-is-associated-with-overexpression-of-c-myc-and-c-ebp%C3%AE-rather-than-enterotoxigenic-bacteroides-fragilis-infection
#15
Anastasiya V Snezhkina, George S Krasnov, Anastasiya V Lipatova, Asiya F Sadritdinova, Olga L Kardymon, Maria S Fedorova, Nataliya V Melnikova, Oleg A Stepanov, Andrew R Zaretsky, Andrey D Kaprin, Boris Y Alekseev, Alexey A Dmitriev, Anna V Kudryavtseva
Colorectal cancer is one of the most common cancers in the world. It is well known that the chronic inflammation can promote the progression of colorectal cancer (CRC). Recently, a number of studies revealed a potential association between colorectal inflammation, cancer progression, and infection caused by enterotoxigenic Bacteroides fragilis (ETBF). Bacterial enterotoxin activates spermine oxidase (SMO), which produces spermidine and H2O2 as byproducts of polyamine catabolism, which, in turn, enhances inflammation and tissue injury...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27270441/molecular-analysis-of-urothelial-cancer-cell-lines-for-modeling-tumor-biology-and-drug-response
#16
M L Nickerson, N Witte, K M Im, S Turan, C Owens, K Misner, S X Tsang, Z Cai, S Wu, M Dean, J C Costello, D Theodorescu
The utility of tumor-derived cell lines is dependent on their ability to recapitulate underlying genomic aberrations and primary tumor biology. Here, we sequenced the exomes of 25 bladder cancer (BCa) cell lines and compared mutations, copy number alterations (CNAs), gene expression and drug response to BCa patient profiles in The Cancer Genome Atlas (TCGA). We observed a mutation pattern associated with altered CpGs and APOBEC-family cytosine deaminases similar to mutation signatures derived from somatic alterations in muscle-invasive (MI) primary tumors, highlighting a major mechanism(s) contributing to cancer-associated alterations in the BCa cell line exomes...
January 5, 2017: Oncogene
https://www.readbyqxmd.com/read/27172220/copy-number-profiling-of-brazilian-astrocytomas
#17
Lucas Tadeu Bidinotto, Raul Torrieri, Alan Mackay, Gisele Caravina Almeida, Marta Viana-Pereira, Adriana Cruvinel-Carloni, Maria Luisa Spina, Nathalia Cristina Campanella, Weder Pereira de Menezes, Carlos Afonso Clara, Aline Paixão Becker, Chris Jones, Rui Manuel Reis
Copy number alterations (CNA) are one of the driving mechanisms of glioma tumorigenesis, and are currently used as important biomarkers in the routine setting. Therefore, we performed CNA profiling of 65 astrocytomas of distinct malignant grades (WHO grade I-IV) of Brazilian origin, using array-CGH and microsatellite instability analysis (MSI), and investigated their correlation with TERT and IDH1 mutational status and clinico-pathological features. Furthermore, in silico analysis using the Oncomine database was performed to validate our findings and extend the findings to gene expression level...
2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27068473/mtap-deletions-in-cancer-create-vulnerability-to-targeting-of-the-mat2a-prmt5-riok1-axis
#18
Katya Marjon, Michael J Cameron, Phong Quang, Michelle F Clasquin, Everton Mandley, Kaiko Kunii, Michael McVay, Sung Choe, Andrew Kernytsky, Stefan Gross, Zenon Konteatis, Joshua Murtie, Michelle L Blake, Jeremy Travins, Marion Dorsch, Scott A Biller, Kevin M Marks
Homozygous deletions of p16/CDKN2A are prevalent in cancer, and these mutations commonly involve co-deletion of adjacent genes, including methylthioadenosine phosphorylase (MTAP). Here, we used shRNA screening and identified the metabolic enzyme, methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion. Metabolomic and biochemical studies revealed a mechanistic basis for this synthetic lethality. The MTAP substrate methylthioadenosine (MTA) accumulates upon MTAP loss...
April 19, 2016: Cell Reports
https://www.readbyqxmd.com/read/26936918/methionine-and-kynurenine-activate-oncogenic-kinases-in-glioblastoma-and-methionine-deprivation-compromises-proliferation
#19
Kamalakannan Palanichamy, Krishnan Thirumoorthy, Suman Kanji, Nicolaus Gordon, Rajbir Singh, John R Jacob, Nikhil Sebastian, Kevin T Litzenberg, Disha Patel, Emily Bassett, Brinda Ramasubramanian, Tim Lautenschlaeger, Steven M Fischer, Abhik Ray-Chaudhury, Arnab Chakravarti
PURPOSE: We employed a metabolomics-based approach with the goal to better understand the molecular signatures of glioblastoma cells and tissues, with an aim toward identifying potential targetable biomarkers for developing more effective and novel therapies. EXPERIMENTAL DESIGN: We used liquid chromatography coupled with mass spectrometry (LC-MS/Q-TOF and LC-MS/QQQ) for the discovery and validation of metabolites from primary and established glioblastoma cells, glioblastoma tissues, and normal human astrocytes...
July 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/26912361/disordered-methionine-metabolism-in-mtap-cdkn2a-deleted-cancers-leads-to-dependence-on-prmt5
#20
Konstantinos J Mavrakis, E Robert McDonald, Michael R Schlabach, Eric Billy, Gregory R Hoffman, Antoine deWeck, David A Ruddy, Kavitha Venkatesan, Jianjun Yu, Gregg McAllister, Mark Stump, Rosalie deBeaumont, Samuel Ho, Yingzi Yue, Yue Liu, Yan Yan-Neale, Guizhi Yang, Fallon Lin, Hong Yin, Hui Gao, D Randal Kipp, Songping Zhao, Joshua T McNamara, Elizabeth R Sprague, Bing Zheng, Ying Lin, Young Shin Cho, Justin Gu, Kenneth Crawford, David Ciccone, Alberto C Vitari, Albert Lai, Vladimir Capka, Kristen Hurov, Jeffery A Porter, John Tallarico, Craig Mickanin, Emma Lees, Raymond Pagliarini, Nicholas Keen, Tobias Schmelzle, Francesco Hofmann, Frank Stegmeier, William R Sellers
5-Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine salvage pathway. The MTAP gene is frequently deleted in human cancers because of its chromosomal proximity to the tumor suppressor gene CDKN2A. By interrogating data from a large-scale short hairpin RNA-mediated screen across 390 cancer cell line models, we found that the viability of MTAP-deficient cancer cells is impaired by depletion of the protein arginine methyltransferase PRMT5. MTAP-deleted cells accumulate the metabolite methylthioadenosine (MTA), which we found to inhibit PRMT5 methyltransferase activity...
March 11, 2016: Science
keyword
keyword
95575
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"