keyword
MENU ▼
Read by QxMD icon Read
search

embryo in vitro production

keyword
https://www.readbyqxmd.com/read/27925005/seed-desiccation-tolerance-and-cryopreservation-of-philippine-calamansi-x-citrofortunella-microcarpa-bunge-wijnands
#1
O L Damasco, L C Refuerzo
BACKGROUND: The traditional on farm conservation of Calamansi [x Citrofortunella microcarpa (Bunge) Wijnands], an important indigenous Citrus species in the Philippines, is now being threatened by shifting agricultural crop production, climate change, and increasing biotic and abiotic stresses. OBJECTIVE: The study aimed to characterize the desiccation and cryopreservation tolerance of seeds as the basis for complementary long term ex situ conservation. MATERIALS AND METHODS: Intact seeds were desiccated in an airtight container filled with activated silica gel for 0-96 h...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27913778/effect-of-deslorelin-acetate-treatment-in-oocyte-recovery-and-in-vitro-embryo-production-in-domestic-cats
#2
Camila Louise Ackermann, Eduardo Trevisol, Leticia Ferrari Crocomo, Tatiana da Silva Rascado, Rodrigo Volpato, Carlos Renato de Freitas Guaitolini, Carlize Lopes, Talita de Almeida Costa, Maria Denise Lopes
OBJECTIVES: The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. METHODS: Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group...
December 2, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27896488/erratum-to-designing-3-dimensional-in-vitro-oviduct-culture-systems-to-study-mammalian-fertilization-and-embryo-production
#3
Marcia A M M Ferraz, Heiko H W Henning, Tom A E Stout, Peter L A M Vos, Bart M Gadella
No abstract text is available yet for this article.
November 28, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27890045/protein-in-culture-and-endogenous-lipid-interact-with-embryonic-stages-in-vitro-to-alter-calf-birthweight-after-embryo-vitrification-and-warming
#4
E Gómez, S Carrocera, S Uzbekova, D Martín, A Murillo, M Alonso-Guervós, F Goyache, M Muñoz
Short-term protein removal in vitro improves long-term blastocyst competence to survive vitrification. We investigated the mechanisms and effects underlying protein removal. Day-6 morulae and early blastocysts were cultured individually with and without protein for 24h. Development and lipid content were analysed in expanded blastocysts derived from morulae (M-XB) and from early blastocysts (EB-XB). Expression of genes involved in lipid metabolism, stress responses and apoptosis was analysed in fresh and vitrified-warmed M-XB produced with and without protein...
November 28, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27862396/research-on-reproduction-is-essential-for-captive-breeding-of-endangered-carnivore-species
#5
K Jewgenow, B C Braun, M Dehnhard, J Zahmel, F Goeritz
Assisted reproductive technology (ART) has great potential for conservation, but its successful application in captive breeding programmes of endangered species is often compromised by limited background on species' biology. Although carnivore species benefit from knowledge obtained in domesticated species (dogs, cats and ferrets), the focus of research is different. In pet animals, research in reproduction has mainly been focused on ovarian function and contraception, although substantial progress has also been made in the field of in vitro embryo production, transgenic embryos and cloning to aid relevant medical models...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27846865/hysteroscopic-management-of-a-heterotopic-pregnancy-following-uterine-artery-embolization-a-case-report
#6
Jigyasa Subedi, Min Xue, Xin Sun, Dabao Xu, Xinliang Deng, Kenan Yu, Xi Yang
BACKGROUND: Intra-uterine pregnancy coexisting with cervical pregnancy (heterotopic pregnancy) is a rare condition and its management is challenging because of the massive bleeding associated with cervical pregnancy. Uterine artery embolization followed by hysteroscopic removal of cervical and intra-uterine products of conception can theoretically prevent massive bleeding and provide a direct view during the removal. Hysteroscopic management following uterine artery embolization of heterotopic pregnancy after in vitro fertilization and embryo transfer is rarely reported...
November 15, 2016: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/27844174/designing-3-dimensional-in-vitro-oviduct-culture-systems-to-study-mammalian-fertilization-and-embryo-production
#7
Marcia A M M Ferraz, Heiko H W Henning, Tom A E Stout, Peter L A M Vos, Bart M Gadella
The oviduct was long considered a largely passive conduit for gametes and embryos. However, an increasing number of studies into oviduct physiology have demonstrated that it specifically and significantly influences gamete interaction, fertilization and early embryo development. While oviduct epithelial cell (OEC) function has been examined during maintenance in conventional tissue culture dishes, cells seeded into these two-dimensional (2-D) conditions suffer a rapid loss of differentiated OEC characteristics, such as ciliation and secretory activity...
November 14, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27829441/characterization-of-exosomal-release-in-bovine-endometrial-intercaruncular-stromal-cells
#8
Yong Qin Koh, Hassendrini N Peiris, Kanchan Vaswani, Sarah Reed, Gregory E Rice, Carlos Salomon, Murray D Mitchell
BACKGROUND: Cell-to-cell communication between the blastocyst and endometrium is critical for implantation. In recent years, evidence has emerged from studies in humans and several other animal species that exosomes are secreted from the endometrium and trophoblast cells and may play an important role in cell-to-cell communication maternal-fetal interface during early pregnancy. Exosomes are stable extracellular lipid bilayer vesicles that encapsulate proteins, miRNAs, and mRNAs, with the ability to deliver their cargo to near and distant sites, altering cellular function(s)...
November 9, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27821054/determination-of-virulence-and-antibiotic-resistance-pattern-of-biofilm-producing-listeria-species-isolated-from-retail-raw-milk
#9
Kamelia M Osman, Ahmed Samir, Usama H Abo-Shama, Essam H Mohamed, Ahmed Orabi, Tara Zolnikov
BACKGROUND: One of the foodborne pathogens is Listeria monocytogenes, which causes serious invasive illness in elderly and immunocompromised patients, pregnant women, newborns and infants ranking second after salmonellosis because of its high case fatality rate. Listerial cow mastitis marked by abnormal milk, increased cell counts and reduced production has not been reported. Therefore, apparently healthy cows can be reservoirs of L. monocytogenes. A number of 203 udder milk samples from apparently healthy animals (buffalo, n = 100; cow, n = 103) were collected and tested for Listeria...
November 8, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27812444/embryogenesis-in-polianthes-tuberosa-l-var-simple-from-megasporogenesis-to-early-embryo-development
#10
Alejandra G González-Gutiérrez, Benjamín Rodríguez-Garay
The genus Polianthes belongs to the subfamily Agavoideae of the Asparagaceae family formerly known as Agavaceae. The genus is endemic to México and comprises about 15 species, among them is Polianthes tuberosa L. The aim of this work was to study and characterize the embryo sac and early embryo development of this species in order to generate basic knowledge for its use in taxonomy, in vitro fertilization and production of haploid plants and to complement studies already performed in other genera and species belonging to the Agavoideae sub-family...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27807889/viability-and-growth-of-feline-preantral-follicles-in-vitro-cultured-with-insulin-growth-factor-and-epidermal-growth-factor-supplemented-medium
#11
A E Alves, L C Padilha-Nakaghi, E A Pires-Butler, M Apparicio, Nam Silva, T F Motheo, Wrr Vicente, G C Luvoni
In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior results of follicular development, antrum formation and proliferation of granulosa cells. However, there are only few reports regarding the use of these factors on feline preantral follicle in vitro culture. Thus, the aim of this study was to investigate the effect of a combination of IGF-1 and EGF on in vitro viability and growth of preantral follicles and enclosed oocytes collected from domestic cats...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27805545/effect-of-sperm-preparation-on-development-of-bovine-blastocyst-in-vitro
#12
Maria Celina Abraham, Anders Johannisson, Jane M Morrell
Sperm preparation is an important step in the in vitro production of embryos. Centrifugation through colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B)...
December 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27799912/understanding-mechanisms-of-gli-mediated-transcription-during-craniofacial-development-and-disease-using-the-ciliopathic-mutant-talpid-2
#13
Ya-Ting Chang, Praneet Chaturvedi, Elizabeth N Schock, Samantha A Brugmann
The primary cilium is a ubiquitous, microtubule-based organelle that cells utilize to transduce molecular signals. Ciliopathies are a group of diseases that are caused by a disruption in the structure or function of the primary cilium. Over 30% of all ciliopathies are primarily defined by their craniofacial phenotypes, which typically include midfacial defects, cleft lip/palate, micrognathia, aglossia, and craniosynostosis. The frequency and severity of craniofacial phenotypes in ciliopathies emphasizes the importance of the cilium during development of the craniofacial complex...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27797719/construction-of-a-series-of-pcs2-backbone-based-gateway-vectors-for-overexpressing-various-tagged-proteins-in-vertebrates
#14
Hong-Yan Wang, Yang Li, Tingling Xue, Ningyan Cheng, Hai-Ning Du
Gateway vectors have been extensively developed to facilitate gene cloning in numerous species; however, a universal system that is compatible for multiple organisms was lacking. As a multipurpose expression vector, pCS2+ backbone-based expression plasmids are widely used for high-level expression of messenger RNAs (mRNAs) or proteins in mammalian/avian culture cells or Xenopus/zebrafish embryos. To date, a suite of vectors with pCS2+ backbone applicable for Gateway cloning system were unavailable yet. Here, we generated a set of Gateway destination vectors, named as pGCS (plasmids of Gateway in pCS2+) vectors, which can be fused to a choice of frequently used amino- or carboxyl-terminal tags, including MYC, HA, FLAG, His, GST, as well as eGFP fluorescent epitope...
December 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27780517/effect-and-possible-mechanisms-of-melatonin-treatment-on-the-quality-and-developmental-potential-of-aged-bovine-oocytes
#15
Shuang Liang, Jing Guo, Jeong-Woo Choi, Nam-Hyung Kim, Xiang-Shun Cui
After reaching the metaphase II (MII) stage, unfertilised oocytes undergo a time-dependent process of quality deterioration referred to as oocyte aging. The associated morphological and cellular changes lead to decreased oocyte developmental potential. This study investigated the effect of exogenous melatonin supplementation on in vitro aged bovine oocytes and explored its underlying mechanisms. The levels of cytoplasmic reactive oxygen species and DNA damage response in bovine oocytes increased during in vitro aging...
October 26, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27780485/meiotic-arrest-as-an-alternative-to-increase-the-production-of-bovine-embryos-by-somatic-cell-nuclear-transfer
#16
F M C Caixeta, R V Sousa, A L Guimarães, L O Leme, J F W Sprícigo, S B Senna Netto, I Pivato, M A N Dode
This study aimed to evaluate the effect of meiotic arrest using phosphodiesterase type 3A (PDE 3A) inhibitors, cilostamide and C-type natriuretic peptide (NPPC), on pre-maturation (PM) of oocytes to be used in the production of cloned embryos. Nuclear maturation, in vitro embryo production (IVP), somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA), and total cells number of cloned embryos were evaluated. The results were analysed by chi-squared and Kruskal-Wallis test with a P-value 0.05) between control and PM, both for cleavage (78...
October 26, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27770559/fluorescent-quantification-of-melanin
#17
Bruno Fernandes, Teresa Matamá, Diana Guimarães, Andreia Gomes, Artur Cavaco-Paulo
Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin...
November 2016: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/27766972/insulin-concentrations-used-in-in-vitro-embryo-production-systems-a-pilot-study-on-insulin-stability-with-an-emphasis-on-concentrations-measured-in-vivo
#18
Denise Laskowski, Ylva Sjunnesson, Hans Gustafsson, Patrice Humblot, Göran Andersson, Renée Båge
BACKGROUND: Insulin has been used as a stimulatory factor for in vitro cell culture since many years. Even for routine in vitro embryo production (IVP), insulin is added to the media during different steps. There is a strong difference in concentrations used in vitro compared to what is measured in vivo in follicular fluid or serum. We performed a pilot study on insulin stability to explain possible reasons for that variation. RESULTS: We measured insulin concentrations before and after bovine oocyte maturation in an experiment by using a quantitative ELISA (Mercodia bovine insulin ELISA immunoassay) and found that concentrations were stable up to 22 h of incubation...
October 20, 2016: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/27756325/less-is-more-low-expression-of-mt1-mmp-is-optimal-to-promote-migration-and-tumourigenesis-of-breast-cancer-cells
#19
Mario A Cepeda, Jacob J H Pelling, Caitlin L Evered, Karla C Williams, Zoey Freedman, Ioana Stan, Jessica A Willson, Hon S Leong, Sashko Damjanovski
BACKGROUND: Membrane Type-1 Matrix Metalloproteinase (MT1-MMP) is a multifunctional protease implicated in metastatic progression ostensibly due to its ability to degrade extracellular matrix (ECM) components and allow migration of cells through the basement membrane. Despite in vitro studies demonstrating this principle, this knowledge has not translated into the use of MMP inhibitors (MMPi) as effective cancer therapeutics, or been corroborated by evidence of in vivo ECM degradation mediated by MT1-MMP, suggesting that our understanding of the role of MT1-MMP in cancer progression is incomplete...
October 18, 2016: Molecular Cancer
https://www.readbyqxmd.com/read/27752179/-effect-of-catheter-choice-during-embryo-transfer-on-the-clinical-outcome-of-in-vitro-fertilization-embryo-transfer
#20
X L Ren, P Liu, Y Lian, J Huang, X Y Zheng, Y J Zhu, J Qiao
OBJECTIVE: To compare two flexible embryo catheters and determine whether clinical outcome differs in the in vitro fertilization-embryo transfer (IVF-ET) cycles. METHODS: This prospective control study was conducted by one doctor between July 2012 and November 2013. In the study, 2 064 patients undergoing fresh embryo transfer by using IVF-ET/intracytoplasmic sperm injection (ICSI)-ET in Reproductive Medical Center of Peking University Third Hospital were recruited...
October 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
keyword
keyword
95563
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"