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embryo in vitro production

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https://www.readbyqxmd.com/read/28629378/microrna-130b-is-involved-in-bovine-granulosa-and-cumulus-cells-function-oocyte-maturation-and-blastocyst-formation
#1
Pritam Bala Sinha, Dawit Tesfaye, Franca Rings, Munir Hossien, Michael Hoelker, Eva Held, Christaine Neuhoff, Ernst Tholen, Karl Schellander, Dessie Salilew-Wondim
BACKGROUND: Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. METHODS: For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse...
June 19, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#2
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28619101/in-vitro-production-of-bovine-embryos-derived-from-individual-donors-in-the-corral-%C3%A2-dish
#3
Maaike Catteeuw, Eline Wydooghe, Erik Mullaart, Hiemke M Knijn, Ann Van Soom
BACKGROUND: Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor. Due to the rather low yield of oocytes collected after ovum pick-up (OPU) per individual cow, oocyte maturation and embryo culture take place in small groups, which is often associated with inferior embryo development. The objective of this study was to improve embryonic development in small donor groups by using the Corral(®) dish...
June 15, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28617821/supplementation-with-small-extracellular-vesicles-from-ovarian-follicular-fluid-during-in-vitro-production-modulates-bovine-embryo-development
#4
Juliano C da Silveira, Gabriella M Andrade, Maite Del Collado, Rafael V Sampaio, Juliano R Sangalli, Luciano A Silva, Fábio V L Pinaffi, Izabelle B Jardim, Marcelo C Cesar, Marcelo F G Nogueira, Aline S M Cesar, Luiz L Coutinho, Rinaldo W Pereira, Felipe Perecin, Flávio V Meirelles
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception. Alternatives to overcome these problems are based on assisted reproductive techniques. Extracellular vesicles are cell-secreted vesicles present in different body fluids and contain bioactive materials, such as messenger RNA, microRNAs (miRNAs), and proteins. Thus, our hypothesis is that extracellular vesicles from follicular fluid from 3-6 mm ovarian follicles can modulate bovine embryo development in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28616913/-the-effect-of-exogenous-recombinant-hmgb1-on-neural-stem-cells-and-related-mechanism
#5
Hui-Min Zhang, Bo Wu, Tian Cao, Yu-Ying Yan, Ming Liu
OBJECTIVES: To explore the effect of exogenous recombinant high mobility group protein box1 (rHMGB1) on proliferation and differentiation of neural stem cells (NSCs) and the related mechanism. METHODS: SD rat cerebral cortex cells were cultured in serum-free medium, extending the culture and purification of neural stem cells. NSCs were identified by detecting nestin-label with immunofluorescence method.The NSCs proliferation activity after adding different concentrations of rHMGB1 was determined by CCK-8 assay and the optimal concentration of rHMGB1 for the follow-up experiments was selected...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28613231/melatonin-scavenger-properties-against-oxidative-and-nitrosative-stress-impact-on-gamete-handling-and-in-vitro-embryo-production-in-humans-and-other-mammals
#6
REVIEW
Pía Loren, Raúl Sánchez, María-Elena Arias, Ricardo Felmer, Jennie Risopatrón, Carolina Cheuquemán
Oxidative and nitrosative stress are common problems when handling gametes in vitro. In vitro development in mammalian embryos is highly affected by culture conditions, especially by reactive oxygen species (ROS) and reactive nitrogen species (RNS), because their absence or overproduction causes embryo arrest and changes in gene expression. Melatonin in gamete co-incubation during in vitro fertilization (IVF) has deleterious or positive effects, depending on the concentration used in the culture medium, demonstrating the delicate balance between antioxidant and pro-oxidant activity...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28613150/toll-like-receptor-tlr-21-signalling-mediated-antiviral-response-against-avian-influenza-virus-infection-correlates-with-macrophage-recruitment-and-nitric-oxide-production
#7
Mohamed Sarjoon Abdul-Cader, Hanaa Ahmed-Hassan, Aruna Amarasinghe, Eva Nagy, Shayan Sharif, Mohamed Faizal Abdul-Careem
Cytosine-guanosinedeoxynucleotide (CpG) DNA can be used for the stimulation of the toll-like receptor (TLR)21 signalling pathway in avian species which ultimately leads to up-regulation of gene transcription for pro-inflammatory molecules including nitric oxide and recruitment of innate immune cells. The objective of this study was to determine the antiviral effect of NO, produced in response to in ovo delivery of CpG DNA, against avian influenza virus (AIV) infection. We found that when CpG DNA is delivered at embryo day (ED)18 in ovo and subsequently challenged with H4N6 AIV at ED19 pre-hatch and day 1 post-hatching, CpG DNA reduces H4N6 AIV replication associated with enhanced NO production and macrophage recruitment in lungs...
June 14, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28601158/effect-of-corifollitropin-alfa-supplemented-with-or-without-lh-on-ovarian-stimulation-and-embryo-viability-in-rabbit
#8
María Pilar Viudes-de-Castro, Francisco Marco-Jiménez, José Isaí Cedano-Castro, José Salvador Vicente
There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601148/leptin-supplementation-in%C3%A2-vitro-improved-developmental-competence-of-buffalo-oocytes-and-embryos
#9
Bibhudatta S K Panda, Sriti Pandey, Anjali Somal, Mehtab S Parmar, Irfan A Bhat, Indu Baiju, Mukesh K Bharti, G Sai Kumar, Vikash Chandra, G Taru Sharma
The aim of the present study was to determine potential role of leptin on in vitro developmental competence of buffalo oocytes and embryos. Slaughterhouse derived culture grade buffalo cumulus oocyte complexes (COCs) were matured in vitro (IVM) with leptin (10 ng/ml) or without leptin (control). In each experiment, a pool of matured COCs was used for further in vitro embryo production and another pool of COCs was used for cumulus cells and mature oocytes isolation to study the relative mRNA expression of developmentally important genes...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28597418/influence-of-led-light-spectra-on-in-vitro-somatic-embryogenesis-and-lc-ms-analysis-of-chlorogenic-acid-and-rutin-in-peucedanum-japonicum-thunb-a-medicinal-herb
#10
Chia-Chen Chen, Dinesh Chandra Agrawal, Maw-Rong Lee, Ren-Jye Lee, Chao-Lin Kuo, Chi-Rei Wu, Hsin-Sheng Tsay, Hung-Chi Chang
BACKGROUND: Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of P. japonicum via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of P. japonicum in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos...
December 2016: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28596081/vitamin-k2-improves-developmental-competency-and-cryo-tolerance-of-in-vitro-derived-ovine-blastocyst
#11
Fatemeh Sefid, S Ostadhosseini, S M Hosseini, F Ghazvini Zadegan, M Pezhman, Mohammad Hossein Nasr Esfahani
Vitamin K2 (VK2), acts as an electron carrier in mitochondria and thereby effects reactive oxygen species (ROS) and ATP production. This study evaluates role of VK2 on in vitro developmental competency and cryo-survival of pre-implantation ovine embryos. Initially the optimal and beneficial concentration of VK2 on compaction and blastocyst formation rates was defined (0.1 μM). Subsequently, it was shown that 0.1 μM VK2, at blastocyst stage, reduces H2O2 production, increase the expression of mitochondrial related gene and improved embryos quality...
June 5, 2017: Cryobiology
https://www.readbyqxmd.com/read/28583610/effects-of-oocytes-exposure-to-bovine-diarrhea-viruses-bvdv-1-bvdv-2-and-hobi-like-virus-on-in%C3%A2-vitro-produced-bovine-embryo-development-and-viral-infection
#12
V da Silva Cardoso Pinto, M F Alves, M de Souza Nunes Martins, A C Basso, J H Tannura, J H F Pontes, M Santos Lima, T Garcia da Silva, L H Okuda, E Stefano, A H C N Romaldini, D R Arnold, E M Pituco
As production of in vitro (IVP) bovine embryos steadily increases, the sanitary risk associated with IVP embryos remains a concern. One of the greatest concerns is how BVDV may be transmitted through IVP embryos. The objective of this study was to evaluate the effects caused by BVDV-1, BVDV-2 and Hobi-like virus exposure during in vitro maturation on embryo development and viral infection. Abittior-derived oocytes were randomly assigned for in vitro maturation with serial concentrations of BVDV-1 (3.12 × 10(2) - 2...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28576621/chelating-agents-in-combination-with-rosmarinic-acid-for-boar-sperm-freeze-drying
#13
Maite Olaciregui, Victoria Luño, Noelia González, Paula Domingo, Ignacio de Blas, Lydia Gil
The presence of DNA protective agents in the medium is necessary to maintain sperm functionality after freeze-drying procedure. The objective of this study was to investigate the effect of chelating agents, ethylene diaminetetraacetic acid (EDTA) and ethylene glycoltetraacetic acid (EGTA), in combination with rosmarinic acid (RA) on DNA integrity of freeze-dried boar sperm. We also examined the effect of these agents on the in vitro developmental ability of porcine oocytes following sperm injection (ICSI). Heterospermic mix, obtained from ejaculated sperm of three boars, was freeze-dried in two different chelating agents' media: 50mM EDTA or 50mM EGTA, and in these media supplemented with 105μM of rosmarinic acid...
May 30, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28555192/the-use-of-xanthan-gum-as-vaccine-adjuvant-an-evaluation-of-immunostimulatory-potential-in-balb-c-mice-and-cytotoxicity-in-vitro
#14
Rodrigo Andrade Schuch, Thaís Larré Oliveira, Thaís Farias Collares, Leonardo Garcia Monte, Guilherme Roig Inda, Odir Antonio Dellagostin, Claire Tondo Vendruscolo, Angelita da Silveira Moreira, Daiane Drawanz Hartwig
The successful production of new, safe, and effective vaccines that generate immunological memory is directly related to adjuvant feature, which is responsible for increasing and/or modulating the immune response. Several compounds display adjuvant activity, including carbohydrates. These compounds play important roles in the immune response, as well as having biocompatible properties in vaccine formulations. One such carbohydrate is xanthan gum, a polysaccharide that is produced by the plant-pathogenic bacterium Xanthomonas spp...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28548038/marek-s-disease-virus-infection-of-phagocytes-a-de-novo-in-vitro-infection-model
#15
Pankaj Chakraborty, Lonneke Vervelde, Robert G Dalziel, Peter S Wasson, Venugopal Nair, Bernadette M Dutia, Pete Kaiser
Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells...
May 26, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28543358/transient-arrest-of-germinal-vesicle-breakdown-improved-in-vitro-development-potential-of-buffalo-bubalus-bubalis-oocytes
#16
Sandeep Kumar, Manish Kumar, Sunny Dholpuria, Parul Sarwalia, Vipul Batra, Sachinandan De, Rakesh Kumar, T K Datta
Germinal vesicle breakdown (GVBD) is the first milestone that an oocyte needs to achieve towards completing the maturation and gaining potential to fertilise. Significantly lower in vitro embryo production rate in buffaloes can be attributed to heterogeneity of GVBD occurrence among oocytes obtained from abattoir derived ovaries. Evidence from our earlier work had suggested that different qualities of buffalo oocytes differ significantly in their timing of GVBD. Besides, these oocytes also differ in terms of volume of Akt phosphorylation, which initiates the process of GVBD...
May 25, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#17
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28518112/functional-manipulation-of-maternal-gene-products-using-in-vitro-oocyte-maturation-in-zebrafish
#18
Elaine L Welch, Celeste C Eno, Sreelaja Nair, Robin E Lindeman, Francisco Pelegri
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into the developing oocyte. Because these events rely on maternal products which typically act very soon after fertilization-that preexist inside the egg, standard approaches for expression and functional reduction involving the injection of reagents into the fertilized egg are typically ineffective. Instead, such manipulations must be performed during oogenesis, prior to or during the accumulation of maternal products...
April 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518085/analysis-of-chromosome-segregation-histone-acetylation-and-spindle-morphology-in-horse-oocytes
#19
Federica Franciosi, Irene Tessaro, Rozenn Dalbies-Tran, Cecile Douet, Fabrice Reigner, Stefan Deleuze, Pascal Papillier, Ileana Miclea, Valentina Lodde, Alberto M Luciano, Ghylene Goudet
The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513460/the-role-of-oxidative-stress-in-female-infertility-and-in-vitro-fertilization
#20
Joanna Wojsiat, Jerzy Korczyński, Marta Borowiecka, Halina Małgorzata Żbikowska
Infertility problem involves many couples of reproductive age. It has been estimated that in Poland 0.7-1.0 million pairs require treatment, while for more than half of them assisted reproduction is the only recommended and effective method. Infertility affects 13 to 15% of the world's population. A major concern is the age-related decline in female fertility even more that often a decision about pregnancy is taken at later age. Recent studies show that increased production of reactive oxygen species is an important factor in etiopathogenesis of pregnancy and affects female reproduction...
May 9, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
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