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embryo in vitro production

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https://www.readbyqxmd.com/read/28727799/atlantic-salmon-cardiac-primary-cultures-an-in-vitro-model-to-study-viral-host-pathogen-interactions-and-pathogenesis
#1
Patricia A Noguera, Bianka Grunow, Matthias Klinger, Katherine Lester, Bertrand Collet, Jorge Del-Pozo
Development of Salmon Cardiac Primary Cultures (SCPCs) from Atlantic salmon pre-hatch embryos and their application as in vitro model for cardiotropic viral infection research are described. Producing SCPCs requires plating of trypsin dissociated embryos with subsequent targeted harvest from 24h up to 3 weeks, of relevant tissues after visual identification. SCPCs are then transferred individually to chambered wells for culture in isolation, with incubation at 15-22°. SCPCs production efficiency was not influenced by embryo's origin (0...
2017: PloS One
https://www.readbyqxmd.com/read/28708512/insulin-during-in%C3%A2-vitro-oocyte-maturation-has-an-impact-on-development-mitochondria-and-cytoskeleton-in-bovine-day-8-blastocysts
#2
Denise Laskowski, Renée Båge, Patrice Humblot, Göran Andersson, Marc-André Sirard, Ylva Sjunnesson
Insulin is a key metabolic hormone that controls energy homeostasis in the body, including playing a specific role in regulating reproductive functions. Conditions associated with hyperinsulinemia can lower developmental rates in bovine in vitro embryo production and are linked to decreased fertility in humans, as in cases of obesity or type 2 diabetes. Embryo quality is important for fertility outcome and it can be assessed by choosing scoring standards for various characteristics, such as developmental stage, quality grade, cell number, mitochondrial pattern or actin cytoskeleton structure...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706533/inhibition-of-histone-h3k9-methylation-by-bix-01294-promotes-stress-induced-microspore-totipotency-and-enhances-embryogenesis-initiation
#3
Eduardo Berenguer, Ivett Bárány, María-Teresa Solís, Yolanda Pérez-Pérez, María C Risueño, Pilar S Testillano
Microspore embryogenesis is a process of cell reprogramming, totipotency acquisition and embryogenesis initiation, induced in vitro by stress treatments and widely used in plant breeding for rapid production of doubled-haploids, but its regulating mechanisms are still largely unknown. Increasing evidence has revealed epigenetic reprogramming during microspore embryogenesis, through DNA methylation, but less is known about the involvement of histone modifications. In this study, we have analyzed the dynamics and possible role of histone H3K9 methylation, a major repressive modification, as well as the effects on microspore embryogenesis initiation of BIX-01294, an inhibitor of histone methylation, tested for the first time in plants, in Brassica napus and Hordeum vulgare...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28693630/developmental-competence-of-dromedary-camel-camelus-dromedarius-oocytes-selected-using-brilliant-cresyl-blue-staining
#4
Mohamed Fathi, Mohamed Ashry, Ali Salama, Magdy R Badr
The objectives of the present studies were to investigate the developmental capacity of dromedary camel oocytes selected by brilliant cresyl blue (BCB) staining and to investigate the expression of select transcripts in germinal vesicle (GV) stage oocytes. These transcripts included BMP15 and GDF9 as important transcripts for folliculogenesis and oocyte development, Zar1 and Mater as maternal transcripts required for embryonic development, Cyclin B1 and CDK1 as cell cycle regulators and Oct4 and STAT3 as transcription factors...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28690171/a-fucoidan-fraction-purified-from-chnoospora-minima-a-potential-inhibitor-of-lps-induced-inflammatory-responses
#5
I P Shanura Fernando, K K Asanka Sanjeewa, Kalpa W Samarakoon, Won Woo Lee, Hyun-Soo Kim, Nalae Kang, P Ranasinghe, Hyi-Seung Lee, You-Jin Jeon
Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50=27.82±0.88μg/ml) and expression of PGE2 through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264...
July 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28680528/protective-effect-of-icariin-on-the-development-of-preimplantation-mouse-embryos-against-hydrogen-peroxide-induced-oxidative-injury
#6
Rong Ye, Songhua Xu, Yue Liu, Lili Pang, Xiuli Lian, Yuhuan Zhong, Yang Su, Shie Wang
During in vitro cultivation of preimplantation embryos, the balance between ROS production and clearance is disturbed and may lead to incompetent embryos, which might be a main reason of IVF-ET failure. Icariin (ICA) is reported to be active in clearing ROS. The present study aimed to investigate whether ICA could reverse H2O2 pretreatment-induced mouse preimplantation embryo development arrest and, furthermore, to study the underlying mechanisms by detecting ROS levels, mitochondrial membrane potential (ΔΨm), and zygotic gene expression...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28679464/linoleic-la-and-linolenic-ala-acid-concentrations-in-follicular-fluid-of-prepubertal-goats-and-their-effect-on-oocyte-in-vitro-maturation-and-embryo-development
#7
Montserrat Roura, María G Catalá, Sandra Soto-Heras, Sondes Hammami, Dolors Izquierdo, Ali Fouladi-Nashta, Maria-Teresa Paramio
In this study we assessed the concentration of linoleic acid (LA) and linolenic acid (ALA) in follicular fluid of prepubertal goats according to follicle size (<3mm or ≥3mm) by gas chromatography and tested the addition of different LA and ALA (LA:ALA) concentration ratios (50:50, 100:50 and 200:50µM) to the IVM medium on embryo development, mitochondrial activity, ATP concentration and relative gene expression (RPL19, ribosomal protein L19; SLC2A1, facilitated glucose transporter 1; ATF4, activating transcription factor 4; GPX1, glutathione peroxidase 1; HSPA5, heat-shock protein family A 70 kDa; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DNMT1, DNA methyltransferase 1; GCLC, glutamate-cysteine ligase catalytic subunit; SOD1, superoxide dismutase 1)...
July 6, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28678404/characterization-of-companion-animal-pluripotent-stem-cells
#8
REVIEW
Y Z Paterson, C Kafarnik, D J Guest
Pluripotent stem cells have the capacity to grow indefinitely in culture and differentiate into derivatives of the three germ layers. These properties underpin their potential to be used in regenerative medicine. Originally derived from early embryos, pluripotent stem cells can now be derived by reprogramming an adult cell back to a pluripotent state. Companion animals such as horses, dogs, and cats suffer from many injuries and diseases for which regenerative medicine may offer new treatments. As many of the injuries and diseases are similar to conditions in humans the use of companion animals for the experimental and clinical testing of stem cell and regenerative medicine products would provide relevant animal models for the translation of therapies to the human field...
July 5, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28674502/treatment-of-abnormal-vaginal-microbiota-before-frozen-embryo-transfer-case-report-and-minireview-to-discuss-the-longitudinal-treatment-efficacy-of-oral-clindamycin
#9
Thor Haahr, Helle O Elbaek, Rita J Laursen, Birgit Alsbjerg, Jørgen S Jensen, Peter Humaidan
Abnormal vaginal microbiota (AVM) or bacterial vaginosis (BV) might negatively impact reproductive outcomes of in vitro fertilization (IVF). However, before randomized controlled trials are initiated to investigate cause and effect, it is necessary to establish the optimal treatment for AVM. Metronidazole seems ineffective to treat the biofilm in AVM; thus, clindamycin could be suggested as a relevant antibiotic agent for future intervention based studies. In the present case report, we present the first longitudinal follow-up of the vaginal microbiota with molecular methods during and after oral clindamycin treatment...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28673989/quadrupling-efficiency-in-production-of-genetically-modified-pigs-through-improved-oocyte-maturation
#10
Ye Yuan, Lee D Spate, Bethany K Redel, Yuchen Tian, Jie Zhou, Randall S Prather, R Michael Roberts
Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28670426/comparative-stepwise-pattern-of-reactive-oxygen-species-production-during-in-vitro-development-of-fertilized-and-nuclear-transferred-goat-embryos
#11
Mehdi Hajian, Sayed Morteza Hosseini, Somayyeh Ostadhosseini, Mohammad Hossein Nasr-Esfahani
BACKGROUND: A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somatic cell nuclear transfer (SCNT). Furthermore, the pattern of ROS production of SCNT embryos were compared with zona free embryos derived from in vitro fertilization (IVF)...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28667127/effect-of-urokinase-type-plasminogen-activator-on-in-vitro-bovine-oocyte-maturation
#12
Mariela Roldán-Olarte, Veronica Maillo, Maria-Jesús Sánchez Calabuig, Paula Beltran-Breña, Dimitrios Rizos, Alfonso Gutierrez-Adan
This study examines the impacts of the urokinase type plasminogen activator (uPA) on the in vitro maturation (IVM) of bovine oocytes. Cumulus-oocyte complexes in IVM medium were treated with uPA, amiloride (an uPA inhibitor), DMSO or left untreated (control group). After 24 h of IVM, oocytes were recovered for testing or were in vitro fertilized and cultured to the blastocyst stage. The factors examined in all groups were: i) oocyte nuclear maturation (Hoëscht staining), ii) oocyte cytoplasmic maturation (cortical granules, CG, distribution assessed by LCA-FITC), iii) oocyte and cumulus cell (CC) gene expression (RT-qPCR), and iv) embryo development (cleavage rate and blastocyst yield)...
June 30, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28662882/ovum-pick-up-interval-in-buffalo-bubalus-bubalis-managed-under-wetland-conditions-in-argentina-effect-on-follicular-population-oocyte-recovery-and-in-vitro-embryo-development
#13
J Konrad, G Clérico, M J Garrido, G Taminelli, M Yuponi, R Yuponi, G Crudeli, M Sansinena
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or 14-day (n=6) OPU interval groups...
June 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28660662/chlorogenic-acid-supplementation-during-in-vitro-maturation-improves-maturation-fertilization-and-developmental-competence-of-porcine-oocytes
#14
T-V Nguyen, F Tanihara, Ltk Do, Y Sato, M Taniguchi, M Takagi, T Van Nguyen, T Otoi
Chlorogenic acid (CGA) is a quinic acid conjugate of caffeic acid, and a phytochemical found in many fruits and beverages that acts as an antioxidant. The present study investigated the effects of CGA supplementation during in vitro maturation (IVM), on in vitro development of porcine oocytes, to improve the porcine in vitro production (IVP) system. Oocytes were matured either without (control) or with CGA (10, 50, 100 and 200 μM). Subsequently, the matured oocytes were fertilized and cultured in vitro for 7 day...
June 28, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28651653/influence-of-nitric-oxide-and-phosphodiesterases-during-in-vitro-maturation-of-bovine-oocytes-on-meiotic-resumption-and-embryo-production
#15
Ramon Cesar Botigelli, Katia Lancellotti Schwarz, Fabiane Gilli Zaffalon, Maite Del Collado, Fernanda Cavallari Castro, Hugo Fernandes, Claudia Lima Verde Leal
This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78...
June 27, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28629378/microrna-130b-is-involved-in-bovine-granulosa-and-cumulus-cells-function-oocyte-maturation-and-blastocyst-formation
#16
Pritam Bala Sinha, Dawit Tesfaye, Franca Rings, Munir Hossien, Michael Hoelker, Eva Held, Christaine Neuhoff, Ernst Tholen, Karl Schellander, Dessie Salilew-Wondim
BACKGROUND: Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. METHODS: For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse...
June 19, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#17
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28619101/in-vitro-production-of-bovine-embryos-derived-from-individual-donors-in-the-corral-%C3%A2-dish
#18
Maaike Catteeuw, Eline Wydooghe, Erik Mullaart, Hiemke M Knijn, Ann Van Soom
BACKGROUND: Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor. Due to the rather low yield of oocytes collected after ovum pick-up (OPU) per individual cow, oocyte maturation and embryo culture take place in small groups, which is often associated with inferior embryo development. The objective of this study was to improve embryonic development in small donor groups by using the Corral(®) dish...
June 15, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28617821/supplementation-with-small-extracellular-vesicles-from-ovarian-follicular-fluid-during-in-vitro-production-modulates-bovine-embryo-development
#19
Juliano C da Silveira, Gabriella M Andrade, Maite Del Collado, Rafael V Sampaio, Juliano R Sangalli, Luciano A Silva, Fábio V L Pinaffi, Izabelle B Jardim, Marcelo C Cesar, Marcelo F G Nogueira, Aline S M Cesar, Luiz L Coutinho, Rinaldo W Pereira, Felipe Perecin, Flávio V Meirelles
Pregnancy success results from the interaction of multiple factors, among them are folliculogenesis and early embryonic development. Failure during these different processes can lead to difficulties in conception. Alternatives to overcome these problems are based on assisted reproductive techniques. Extracellular vesicles are cell-secreted vesicles present in different body fluids and contain bioactive materials, such as messenger RNA, microRNAs (miRNAs), and proteins. Thus, our hypothesis is that extracellular vesicles from follicular fluid from 3-6 mm ovarian follicles can modulate bovine embryo development in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28616913/-the-effect-of-exogenous-recombinant-hmgb1-on-neural-stem-cells-and-related-mechanism
#20
Hui-Min Zhang, Bo Wu, Tian Cao, Yu-Ying Yan, Ming Liu
OBJECTIVES: To explore the effect of exogenous recombinant high mobility group protein box1 (rHMGB1) on proliferation and differentiation of neural stem cells (NSCs) and the related mechanism. METHODS: SD rat cerebral cortex cells were cultured in serum-free medium, extending the culture and purification of neural stem cells. NSCs were identified by detecting nestin-label with immunofluorescence method.The NSCs proliferation activity after adding different concentrations of rHMGB1 was determined by CCK-8 assay and the optimal concentration of rHMGB1 for the follow-up experiments was selected...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
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