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embryo in vitro production

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https://www.readbyqxmd.com/read/28241436/gomisin-n-inhibits-melanogenesis-through-regulating-the-pi3k-akt-and-mapk-erk-signaling-pathways-in-melanocytes
#1
Jae Kyoung Chae, Lalita Subedi, Minsun Jeong, Yong Un Park, Chul Young Kim, Hakwon Kim, Sun Yeou Kim
Gomisin N, one of the lignan compounds found in Schisandra chinensis has been shown to possess anti-oxidative, anti-tumorigenic, and anti-inflammatory activities in various studies. Here we report, for the first time, the anti-melenogenic efficacy of Gomisin N in mammalian cells as well as in zebrafish embryos. Gomisin N significantly reduced the melanin content without cellular toxicity. Although it was not capable of modulating the catalytic activity of mushroom tyrosinase in vitro, Gomisin N downregulated the expression levels of key proteins that function in melanogenesis...
February 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28237324/in%C3%A2-vitro-production-of-embryos-using-immature-oocytes-collected-transvaginally-from-superstimulated-wood-bison-bison-bison-athabascae
#2
Miriam P Cervantes, J Manuel Palomino, Muhammad Anzar, Reuben J Mapletoft, Gabriela F Mastromonaco, Gregg P Adams
Two experiments were done to test the hypothesis that morphologic characteristics of wood bison cumulus-oocyte complexes (COC) are reflective of the ability of the oocyte to develop to an advanced embryonic stage after in vitro maturation, fertilization and culture, and to determine the effect of prolonging the interval from the end of superstimulation treatment to oocyte collection (FSH starvation period). Experiments were done during the anovulatory season. In Experiment 1, ovarian superstimulation was induced in 10 bison with two doses of FSH given at 48 h intervals beginning at the time of follicular wave emergence...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28236810/count-bayesian-models-for-genetic-analysis-of-in-vitro-embryo-production-traits-in-guzer%C3%A3-cattle
#3
B C Perez, F F Silva, R V Ventura, F A T Bruneli, J C C Balieiro, M G D C Peixoto
Four models for in vitro embryo production traits in Guzerá cattle were compared: Gaussian (untransformed variable - LIN and transformed in logarithmic scale - LOG), Poisson (POI) and zero-inflated Poisson (ZIP). Data consisted of 5716 ovum pick-up and in vitro fertilization records performed in 1205 cows from distinct regions of Brazil. Analyzed count traits were the number of viable oocytes (NOV), number of grade I oocytes (NGI), number of degenerated oocytes (NDG), number of cleaved embryos (NCLV) and number of viable produced embryos (NEMB)...
February 27, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28235674/thrombopoietin-contributes-to-the-formation-and-the-maintenance-of-hematopoietic-progenitor-containing-cell-clusters-in-the-aorta-gonad-mesonephros-region
#4
Kaho Harada, Ikuo Nobuhisa, Maha Anani, Kiyoka Saito, Tetsuya Taga
In the midgestation mouse embryo, hematopoietic cell clusters containing hematopoietic stem/progenitor cells arise in the aorta-gonad-mesonephros (AGM) region. We have previously reported that forced expression of the Sox17 transcription factor in CD45(low)c-Kit(high) AGM cells, which are the hematopoietic cellular component of the cell clusters, and subsequent coculture with OP9 stromal cells in the presence of three cytokines, stem cell factor (SCF), interleukin-3 (IL-3), and thrombopoietin (TPO), led to the formation and the maintenance of cell clusters with cells at an undifferentiated state in vitro...
February 21, 2017: Cytokine
https://www.readbyqxmd.com/read/28215678/the-impact-of-ovarian-stimulation-protocol-on-oocyte-quality-subsequent-in%C3%A2-vitro-embryo-development-and-pregnancy-after-transfer-to-recipients-in-eld-s-deer-rucervus-eldii-thamin
#5
Ampika Thongphakdee, Debra K Berg, Theerawat Tharasanit, Nikorn Thongtip, Wanlaya Tipkantha, Chainarong Punkong, Daraka Tongthainan, Sakhon Noimoon, Umaporn Maikeaw, Nudthakamol Kajornklin, Boripat Siriaroonrat, Pierre Comizzoli, Sumate Kamolnorranath
Propagating genetically valuable individuals through oocyte collection, in vitro fertilization (IVF) and embryo transfer is critical to maintain sustainable populations of the endangered Eld's deer. The objectives of this study were to assess the impact of exogenous FSH injections on (1) the number and in vitro competence of oocytes collected and (2) the developmental potential of resulting IVF embryos after transfer into recipients during the breeding season (February-April). In a pilot experiment, estrus synchronization was conducted in three surplus females (using intravaginal progesterone-releasing devices, CIDRG for 14 days and injections of buserelin (a GnRH agonist)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28209214/effects-of-concentrate-type-and-chromium-propionate-on-insulin-sensitivity-productive-and-reproductive-parameters-of-lactating-dairy-cows-consuming-excessive-energy
#6
T Leiva, R F Cooke, A P Brandão, U Pardelli, R O Rodrigues, F N Corrá, J L M Vasconcelos
This experiment compared insulin sensitivity parameters, milk production and reproductive outcomes in lactating dairy cows consuming excessive energy, and receiving in a 2×2 factorial arrangement design: (1) concentrate based on ground corn (CRN; n=13) or citrus pulp (PLP; n=13), and (2) supplemented (n=14) or not (n=12) with 2.5 g/day of chromium (Cr)-propionate. During the experiment (day 0 to 182), 26 multiparous, non-pregnant, lactating Gir×Holstein cows (initial days in milk=80±2) were offered corn silage for ad libitum consumption, and individually received concentrate formulated to allow diets to provide 160% of their daily requirements of net energy for lactation...
March 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28198080/proteomic-profiling-of-mature-leaves-from-oil-palm-elaeis-guineensis-jacq
#7
Hooi Sin Tan, Richard P Jacoby, Meilina Ong-Abdullah, Nicolas L Taylor, Susan Liddell, Wong Wei Chee, Chiew Foan Chin
Oil palm is one of the most productive oil bearing crops grown in Southeast Asia. Due to the dwindling availability of agricultural land and increasing demand for high yielding oil palm seedlings, clonal propagation is vital to the oil palm industry. Most commonly, leaf explants are used for in vitro micropropagation of oil palm and to optimise this process it is important to unravel the physiological and molecular mechanisms underlying somatic embryo production from leaves. In this study, a proteomic approach was used to determine protein abundance of mature oil palm leaves...
February 15, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28194463/improved-bovine-embryo-production-in-an-oviduct-on-a-chip-system-prevention-of-poly-spermic-fertilization-and-parthenogenic-activation
#8
Marcia A M M Ferraz, Heiko H W Henning, Pedro F Costa, Jos Malda, Ferry P Melchels, R Wubbolts, Tom A E Stout, Peter L A M Vos, Bart M Gadella
The oviduct provides the natural micro-environment for gamete interaction, fertilization and early embryo development in mammals, such as the cow. In conventional culture systems, bovine oviduct epithelial cells (BOEC) undergo a rapid loss of essential differentiated cell properties; we aimed to develop a more physiological in vitro oviduct culture system capable of supporting fertilization. U-shaped chambers were produced using stereo-lithography and mounted with polycarbonate membranes, which were used as culture inserts for primary BOECs...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28166990/use-of-fsh-in-two-different-regimens-for-ovarian-superstimulation-prior-to-ovum-pick-up-and-in%C3%A2-vitro-embryo-production-in-holstein-cows
#9
Júlio César Barboza da Silva, Roberta Machado Ferreira, Milton Maturana Filho, Julianne de Rezende Naves, Thiago Santin, Guilherme Pugliesi, Ed Hoffmann Madureira
We aimed with the present study to evaluate the effects of FSH treatment (200 mg) split in four or six administrations on ovarian follicle stimulation and in vitro oocyte competence for embryo production in dairy cows with synchronized follicular wave emergence. On random days of the estrous cycle (Day 0), non-lactating Holstein cows received a progesterone (P4)-releasing intravaginal device and 2 mg estradiol benzoate IM. On Day 3, they received 0.530 mg sodium cloprostenol (PGF2α) IM. Control cows (n = 35) received no further treatments, whereas FSH-treated cows received 200 mg FSH split in four (FSH4 group; n = 33) or six (FSH6 group; n = 33) administration regimens...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166988/beef-donor-cows-with-high-number-of-retrieved-coc-produce-more-in%C3%A2-vitro-embryos-compared-with-cows-with-low-number-of-coc-after-repeated-ovum-pick-up-sessions
#10
F M Monteiro, E O S Batista, L M Vieira, B M Bayeux, M Accorsi, S P Campanholi, E A R Dias, A H Souza, P S Baruselli
The aim of the present study was to evaluate whether repeated ovum pick-up (OPU) procedures may affect the efficiency of OPU programs for in vitro embryo production (IVEP) in Bos indicus (Nelore) donors. In addition, the repeatability (r) efficiency of IVEP was also assessed. Data available were from 432 OPU-IVEP sessions that were performed at random stages of the estrous cycle in 36 cycling, nonlactating Nelore donors. Semen from three Nelore bulls was used for the IVF. Donors were submitted to 12 consecutive OPU procedures, with an interval of approximately 30 days between sessions...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28163265/significant-heparin-effect-on-bovine-embryo-development-during-sexed-in-vitro-fertilization
#11
Li-You An, Sanjeev A Chaubal, Yanhong Liu, Yexiang Chen, Tshimangadzo L Nedambale, Jie Xu, Fei Xue, Juan F Moreno, Shenghong Tao, Giorgio Antonio Presicce, Fuliang DU
The aim of this study was to investigate the effect of different heparin concentrations in the course of sexed in vitro fertilization (IVF), on bovine embryonic development and development to term following embryo transfer (ET). With a total of 9156 oocytes for IVF, sorted as well as unsorted sperm from four bulls had different heparin requirements for achieving the highest rate of development in vitro. However, when optimal heparin concentrations were used (40 to 80 µg/ml), the performance of X-sorted sperm (0...
February 5, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28137339/satellite-dna-methylation-status-and-expression-of-selected-genes-in-bos-indicus-blastocysts-produced-in-vivo-and-in-vitro
#12
R Urrego, S M Bernal-Ulloa, N A Chavarría, E Herrera-Puerta, A Lucas-Hahn, D Herrmann, S Winkler, D Pache, H Niemann, N Rodriguez-Osorio
Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo...
January 31, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28132145/agrobacterium-mediated-transformation-of-leaf-base-segments
#13
Sebastian Gasparis
Agrobacterium-mediated transformation has become a routine method of genetic engineering of cereals, gradually replacing the biolistic protocols. Simple integration patterns of transgenic loci, decent transformation efficiency, and technical simplicity are the main advantages offered by this method. Here we present a detailed protocol for the production of transgenic oat plants by Agrobacterium-mediated transformation of leaf base segments. The use of leaf explants as target tissues for transformation and in vitro regeneration of transgenic plants may be a good alternative for genotypes which are not susceptible to regeneration from immature or mature embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28132140/oat-doubled-haploids-following-maize-pollination
#14
Philip A Davies, Parminder K Sidhu
Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. These embryos can be cultured in vitro using the "embryo rescue" technique to produce haploid plants whose chromosome number can be doubled with colchicine to produce homozygous DH oat plants.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28122589/escherichia-coli-o78-isolated-from-septicemic-lambs-shows-high-pathogenicity-in-a-zebrafish-model
#15
Cecilie K Kjelstrup, Amelia E Barber, J Paul Norton, Matthew A Mulvey, Trine M L'Abée-Lund
The pathogenicity of Escherichia coli O78 strain K46, originally isolated from an outbreak of septicemia in neonatal lambs, was investigated in zebrafish embryo and murine models of infection. Its biofilm potential, cellulose production, and the expression of type 1 pili and curli fimbriae were measured by in vitro assays. The strain was highly pathogenic in the zebrafish embryo model of infection, where it killed all embryos within 24 h post inoculation (hpi) at doses as low as 1000 colony forming units. Zebrafish embryos inoculated with similar doses of commensal E...
January 25, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28122009/pre-and-peri-post-compaction-follistatin-treatment-increases-in-vitro-production-of-cattle-embryos
#16
Guo Zhenhua, Sandeep K Rajput, Joseph K Folger, Liu Di, Jason G Knott, George W Smith
Our previous studies demonstrated that maternal (oocyte derived) follistatin (FST) expression is positively associated with bovine oocyte competence and exogenous follistatin treatment during the pre-compaction period of development (d 1-3 post insemination) is stimulatory to bovine early embryogenesis in vitro [blastocyst rates and cell numbers/allocation to trophectoderm (TE)]. In the present study, bovine embryos were treated with exogenous follistatin during d 1-3, d 4-7 and d 1-7 post insemination to test the hypothesis that embryotropic effects of exogenous follistatin are specific to the pre-compaction period (d 1-3) of early embryogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28120355/transporting-bovine-oocytes-in-a-medium-supplemented-with-different-macromolecules-and-antioxidants-effects-on-nuclear-and-cytoplasmic-maturation-and-embryonic-development-in-vitro
#17
M Ambrogi, P C Dall'Acqua, Nas Rocha-Frigoni, Bcs Leão, G Z Mingoti
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox)...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28118357/endochondral-ossification-is-accelerated-in-cholinesterase-deficient-mice-and-in-avian-mesenchymal-micromass-cultures
#18
Janine Spieker, Thomas Mudersbach, Astrid Vogel-Höpker, Paul G Layer
Most components of the cholinergic system are detected in skeletogenic cell types in vitro, yet the function of this system in skeletogenesis remains unclear. Here, we analyzed endochondral ossification in mutant murine fetuses, in which genes of the rate-limiting cholinergic enzymes acetyl- (AChE), or butyrylcholinesterase (BChE), or both were deleted (called here A-B+, A+B-, A-B-, respectively). In all mutant embryos bone growth and cartilage remodeling into mineralizing bone were accelerated, as revealed by Alcian blue (A-blu) and Alizarin red (A-red) staining...
2017: PloS One
https://www.readbyqxmd.com/read/28108942/compartmentalized-culture-of-perivascular-stroma-and-endothelial-cells-in-a-microfluidic-model-of-the-human-endometrium
#19
Juan S Gnecco, Virginia Pensabene, David J Li, Tianbing Ding, Elliot E Hui, Kaylon L Bruner-Tran, Kevin G Osteen
The endometrium is the inner lining of the uterus. Following specific cyclic hormonal stimulation, endometrial stromal fibroblasts (stroma) and vascular endothelial cells exhibit morphological and biochemical changes to support embryo implantation and regulate vascular function, respectively. Herein, we integrated a resin-based porous membrane in a dual chamber microfluidic device in polydimethylsiloxane that allows long term in vitro co-culture of human endometrial stromal and endothelial cells. This transparent, 2-μm porous membrane separates the two chambers, allows for the diffusion of small molecules and enables high resolution bright field and fluorescent imaging...
January 20, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28108032/disruption-of-the-sheep-bmpr-ib-gene-by-crispr-cas9-in-in%C3%A2-vitro-produced-embryos
#20
Xuemei Zhang, Wenrong Li, Yangsheng Wu, Xinrong Peng, Bian Lou, Liqin Wang, Mingjun Liu
BMPR-IB (also known as FecB) is a key candidate gene for the genetic control of sheep reproductive performance. Loss-of-function mutations in the sheep BMPR-IB gene lead to an increase in ovulation rate and consequently larger litter size. However, the BMPR-IB gene has been identified in only a few sheep breeds. To improve sheep reproduction through modification of the BMPR-IB gene, we designed an sgRNA to target the sheep BMPR-IB gene by using the CRISPR/Cas9 system. First, we performed gene editing by injecting Cas9/sgRNA into the cytoplasm of one-cell fertilized eggs...
March 15, 2017: Theriogenology
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