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embryo in vitro production

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https://www.readbyqxmd.com/read/28339853/prosurvival-effect-of-cumulus-prostaglandin-g-h-synthase-2-prostaglandin2-signaling-on-bovine-blastocyst-impact-on-in-vivo-posthatching-development%C3%A2
#1
Fabienne Nuttinck, Alice Jouneau, Gilles Charpigny, Isabelle Hue, Christophe Richard, Pierre Adenot, Sylvie Ruffini, Ludivine Laffont, Martine Chebrout, Véronique Duranthon, Brigitte Marquant-Le Guienne
Apoptotic activity is a common physiological process which culminates at the blastocyst stage in the preimplantation embryo of many mammals. The degree of embryonic cell death can be influenced by the oocyte microenvironment. However, the prognostic significance of the incidence of apoptosis remains undefined. Prostaglandin E2 (PGE2) derived from prostaglandin G/H synthase-2 (PTGS2) activity is a well-known prosurvival factor that is mainly studied in oncology. PGE2 is the predominant PTGS2-derived prostaglandin present in the oocyte microenvironment during the periconceptional period...
March 7, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28339479/mouse-newborn-cells-allow-highly-productive-mouse-cytomegalovirus-replication-constituting-a-novel-convenient-primary-cell-culture-system
#2
Vu Thuy Khanh Le-Trilling, Mirko Trilling
Mammalian cell culture is indispensable for most aspects of current biomedical research. Immortalized cell lines are very convenient, but transforming principles (e.g. oncogenic viruses or their oncogenes) can heavily influence the experimental outcome. Primary cells do not share this apparent disadvantage but are more laborious to generate. Certain viruses (e.g. mouse cytomegalovirus) do not replicate efficiently in most transformed cell lines. In the past, such viruses have been routinely propagated on primary mouse embryonic fibroblasts (MEF) established around day 17 (d17) of gestation...
2017: PloS One
https://www.readbyqxmd.com/read/28333031/new-approaches-regarding-the-in-vitro-maturation-of-oocytes-manipulating-cyclic-nucleotides-and-their-partners-in-crime
#3
REVIEW
Ramon Cesar Botigelli, Eduardo Montanari Razza, Elisa Mariano Pioltine, Marcelo Fábio Gouveia Nogueira
Several discoveries have been described recently (5-10 years) about the biology of ovarian follicles (oocyte, cumulus cells and granulosa cells), including new aspects of cellular communication, the control of oocyte maturation and the acquisition of oocyte competence for fertilization and further embryo development. These advances are nourishing assisted reproduction techniques (ART) with new possibilities, in which novel culture systems are being developed and tested to improve embryo yield and quality. This mini-review aims to describe how the recent knowledge on the physiological aspects of mammalian oocyte is reflecting as original or revisited approaches into the context of embryo production...
February 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28331195/galactose-and-its-metabolites-deteriorate-metaphase-ii-mouse-oocyte-quality-and-subsequent-embryo-development-by-disrupting-the-spindle-structure
#4
Mili Thakur, Faten Shaeib, Sana N Khan, Hamid-Reza Kohan-Ghadr, Roohi Jeelani, Sarah R Aldhaheri, Bernard Gonik, Husam M Abu-Soud
Premature ovarian insufficiency (POI) is a frequent long-term complication of classic galactosemia. The majority of women with this disorder develop POI, however rare spontaneous pregnancies have been reported. Here, we evaluate the effect of D-galactose and its metabolites, galactitol and galactose 1-phosphate, on oocyte quality as well as embryo development to elucidate the mechanism through which these compounds mediate oocyte deterioration. Metaphase II mouse oocytes (n = 240), with and without cumulus cells (CCs), were exposed for 4 hours to D-galactose (2 μM), galactitol (11 μM) and galactose 1-phosphate (0...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330290/successful-cloning-of-an-adult-breeding-boar-from-the-novel-chinese-guike-no-1-swine-specialized-strain
#5
Jun-Yu Nie, Xiang-Xing Zhu, Bing-Kun Xie, Su-Qun Nong, Qing-Yan Ma, Hui-Yan Xu, Xiao-Gan Yang, Yang-Qing Lu, Ke-Huan Lu, Yu-Ying Liao, Sheng-Sheng Lu
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed is a novel swine specialized strain incorporated with the pedigree background of Duroc and Chinese Luchuan pig breeds, thus inherits an excellent production performance. The present study was conducted to establish somatic cloning procedures of adult breeding boars from the Chinese Guike No...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28301674/single-cell-transcriptome-profiling-revealed-differences-in-gene-expression-during-oocyte-maturation-in-haimen-white-goats
#6
X Y Yin, G H Cheng, H Y Guo, Q Wang, Y J Li, H Zhang
Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as those from female goats during in vitro maturation. To identify differences during different stages of oocyte maturation, we used single cell transcriptome sequencing to compare gene expression in mature oocytes from kids and female goats...
March 15, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28295710/effects-of-supplementation-of-medium-with-different-antioxidants-during-in-vitro-maturation-of-bovine-oocytes-on-subsequent-embryo-production
#7
T C Sovernigo, P R Adona, P S Monzani, S Guemra, Fda Barros, F G Lopes, Clv Leal
The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse-derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28273380/an-aanat-asmt-transgenic-animal-model-constructed-with-crispr-cas9-system-serving-as-the-mammary-gland-bioreactor-to-produce-melatonin-enrich-milk-in-sheep
#8
Teng Ma, Jingli Tao, Minghui Yang, Changjiu He, Xiuzhi Tian, Xiaosheng Zhang, Jinlong Zhang, Shoulong Deng, Jianzhong Feng, Zhenzhen Zhang, Jing Wang, Pengyun Ji, Yukun Song, Pingli He, Hongbing Han, Juncai Fu, Zhengxing Lian, Guoshi Liu
Melatonin as a potent antioxidant exhibits important nutritional and medicinal values. To produce melatonin-enriching milk will benefit to the consumers. In this study, a sheep bioreactor which generates melatonin-enriching milk has been successfully developed by the technology that combined CRISPR/Cas9 system and microinjection. The AANAT and ASMT were cloned from pineal gland of Dorper sheep (Ovis aries). The in vitro studies found that AANAT and ASMT were successfully transferred to the mammary epithelial cell lines and significantly increased melatonin production in the culture medium compared to the non-transgenic cell lines...
March 8, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28273182/protocol-for-efficient-regulation-of-in-vitro-morphogenesis-in-einkorn-triticum-monococcum-l-a-recalcitrant-diploid-wheat-species
#9
Dmitry Miroshnichenko, Inna Chaban, Mariya Chernobrovkina, Sergey Dolgov
Einkorn (Triticum monococcum L.) is A-genome diploid wheat that has a potential to become a useful model for understanding the biology and genomics in Triticeae. Unfortunately, the application of modern technologies such as genetic engineering, RNAi-based gene silencing and genome editing is not available for einkorn as there is no efficient in vitro tissue culture and plant regeneration system. In the present study an efficient and simple protocol for plant regeneration via direct or indirect somatic embryogenesis and organogenesis has been developed...
2017: PloS One
https://www.readbyqxmd.com/read/28270748/huntingtin-is-required-for-neural-but-not-cardiac-pancreatic-progenitor-differentiation-of-mouse-embryonic-stem-cells-in-vitro
#10
Man Shan Yu, Naoko Tanese
Mutation in the huntingtin (HTT) gene causes Huntington's disease (HD). It is an autosomal dominant trinucleotide-repeat expansion disease in which CAG repeat sequence expands to >35. This results in the production of mutant HTT protein with an increased stretch of glutamines near the N-terminus. The wild type HTT gene encodes a 350 kD protein whose function remains elusive. Mutant HTT protein has been implicated in transcription, axonal transport, cytoskeletal structure/function, signal transduction, and autophagy...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28266561/nkx2-1-regulates-the-generation-of-telencephalic-astrocytes-during-embryonic-development
#11
Shilpi Minocha, Delphine Valloton, Yvan Arsenijevic, Jean-René Cardinaux, Raffaella Guidi, Jean-Pierre Hornung, Cécile Lebrand
The homeodomain transcription factor Nkx2.1 (NK2 homeobox 1) controls cell differentiation of telencephalic GABAergic interneurons and oligodendrocytes. Here we show that Nkx2.1 also regulates astrogliogenesis of the telencephalon from embryonic day (E) 14.5 to E16.5. Moreover we identify the different mechanisms by which Nkx2.1 controls the telencephalic astrogliogenesis. In Nkx2.1 knockout (Nkx2.1(-/-)) mice a drastic loss of astrocytes is observed that is not related to cell death. Further, in vivo analysis using BrdU incorporation reveals that Nkx2...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28263526/bioindicators-of-wastewater-ecotoxicity
#12
REVIEW
Gabriela Jirova, Zdenka Wittlingerova, Magdalena Zimova, Alena Vlkova, Martina Wittlerova, Marketa Dvorakova, Dagmar Jirova
Wastewater, especially containing hospital effluents, exhibits high chemical complexity and specificity since it includes various chemicals, biocides, pharmaceuticals, surfactants, radionuclides, disinfectants and pathogens. Biological tests provide true evidence of the wastewater quality and unlike chemical analytical tests show comprehensive pollution effects on the environment and human health. Normalized conventional bioassays are not sensitive enough for ecotoxicological evaluation of wastewater and there is a great need for the development of suitable sensitive bioassays in order to characterize properly the residual toxicity of treated effluents...
December 18, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28260557/the-influence-of-in-vitro-fertilization-and-embryo-culture-on-the-embryo-epigenetic-constituents-and-the-possible-consequences-in-the-bovine-model
#13
M-A Sirard
Medically assisted reproductive technologies, such as in vitro embryo production, are increasingly being used to palliate infertility. Eggs are produced following a hormonal regimen that stimulates the ovaries to produce a large number of oocytes. Collected oocytes are then fertilized in vitro and allowed to develop in vitro until they are either frozen or transferred to mothers. There are controversial reports on the adverse impacts of these technologies on early embryos and their potential long-term effects...
March 6, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28242961/endometrial-polyps-and-subfertility
#14
REVIEW
Ali Al Chami, Ertan Saridogan
Endometrial polyps are frequently seen in subfertile women, and there is some evidence suggesting a detrimental effect on fertility. How polyps contribute to subfertility and pregnancy loss is uncertain and possible mechanisms are poorly understood. It may be related to mechanical interference with sperm transport, embryo implantation or through intrauterine inflammation or altered production of endometrial receptivity factors. Different diagnostic modalities such as two- or three-dimensional transvaginal ultrasound, saline infusion sonography or hysteroscopy are commonly used to evaluate endometrial polyps with good detection rates...
February 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28241436/gomisin-n-inhibits-melanogenesis-through-regulating-the-pi3k-akt-and-mapk-erk-signaling-pathways-in-melanocytes
#15
Jae Kyoung Chae, Lalita Subedi, Minsun Jeong, Yong Un Park, Chul Young Kim, Hakwon Kim, Sun Yeou Kim
Gomisin N, one of the lignan compounds found in Schisandra chinensis has been shown to possess anti-oxidative, anti-tumorigenic, and anti-inflammatory activities in various studies. Here we report, for the first time, the anti-melenogenic efficacy of Gomisin N in mammalian cells as well as in zebrafish embryos. Gomisin N significantly reduced the melanin content without cellular toxicity. Although it was not capable of modulating the catalytic activity of mushroom tyrosinase in vitro, Gomisin N downregulated the expression levels of key proteins that function in melanogenesis...
February 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28237324/in%C3%A2-vitro-production-of-embryos-using-immature-oocytes-collected-transvaginally-from-superstimulated-wood-bison-bison-bison-athabascae
#16
Miriam P Cervantes, J Manuel Palomino, Muhammad Anzar, Reuben J Mapletoft, Gabriela F Mastromonaco, Gregg P Adams
Two experiments were done to test the hypothesis that morphologic characteristics of wood bison cumulus-oocyte complexes (COC) are reflective of the ability of the oocyte to develop to an advanced embryonic stage after in vitro maturation, fertilization and culture, and to determine the effect of prolonging the interval from the end of superstimulation treatment to oocyte collection (FSH starvation period). Experiments were done during the anovulatory season. In Experiment 1, ovarian superstimulation was induced in 10 bison with two doses of FSH given at 48 h intervals beginning at the time of follicular wave emergence...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28236810/count-bayesian-models-for-genetic-analysis-of-in-vitro-embryo-production-traits-in-guzer%C3%A3-cattle
#17
B C Perez, F F Silva, R V Ventura, F A T Bruneli, J C C Balieiro, M G D C Peixoto
Four models for in vitro embryo production traits in Guzerá cattle were compared: Gaussian (untransformed variable - LIN and transformed in logarithmic scale - LOG), Poisson (POI) and zero-inflated Poisson (ZIP). Data consisted of 5716 ovum pick-up and in vitro fertilization records performed in 1205 cows from distinct regions of Brazil. Analyzed count traits were the number of viable oocytes (NOV), number of grade I oocytes (NGI), number of degenerated oocytes (NDG), number of cleaved embryos (NCLV) and number of viable produced embryos (NEMB)...
February 27, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28235674/thrombopoietin-contributes-to-the-formation-and-the-maintenance-of-hematopoietic-progenitor-containing-cell-clusters-in-the-aorta-gonad-mesonephros-region
#18
Kaho Harada, Ikuo Nobuhisa, Maha Anani, Kiyoka Saito, Tetsuya Taga
In the midgestation mouse embryo, hematopoietic cell clusters containing hematopoietic stem/progenitor cells arise in the aorta-gonad-mesonephros (AGM) region. We have previously reported that forced expression of the Sox17 transcription factor in CD45(low)c-Kit(high) AGM cells, which are the hematopoietic cellular component of the cell clusters, and subsequent coculture with OP9 stromal cells in the presence of three cytokines, stem cell factor (SCF), interleukin-3 (IL-3), and thrombopoietin (TPO), led to the formation and the maintenance of cell clusters with cells at an undifferentiated state in vitro...
February 21, 2017: Cytokine
https://www.readbyqxmd.com/read/28215678/the-impact-of-ovarian-stimulation-protocol-on-oocyte-quality-subsequent-in%C3%A2-vitro-embryo-development-and-pregnancy-after-transfer-to-recipients-in-eld-s-deer-rucervus-eldii-thamin
#19
Ampika Thongphakdee, Debra K Berg, Theerawat Tharasanit, Nikorn Thongtip, Wanlaya Tipkantha, Chainarong Punkong, Daraka Tongthainan, Sakhon Noimoon, Umaporn Maikeaw, Nudthakamol Kajornklin, Boripat Siriaroonrat, Pierre Comizzoli, Sumate Kamolnorranath
Propagating genetically valuable individuals through oocyte collection, in vitro fertilization (IVF) and embryo transfer is critical to maintain sustainable populations of the endangered Eld's deer. The objectives of this study were to assess the impact of exogenous FSH injections on (1) the number and in vitro competence of oocytes collected and (2) the developmental potential of resulting IVF embryos after transfer into recipients during the breeding season (February-April). In a pilot experiment, estrus synchronization was conducted in three surplus females (using intravaginal progesterone-releasing devices, CIDRG for 14 days and injections of buserelin (a GnRH agonist)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28209214/effects-of-concentrate-type-and-chromium-propionate-on-insulin-sensitivity-productive-and-reproductive-parameters-of-lactating-dairy-cows-consuming-excessive-energy
#20
T Leiva, R F Cooke, A P Brandão, U Pardelli, R O Rodrigues, F N Corrá, J L M Vasconcelos
This experiment compared insulin sensitivity parameters, milk production and reproductive outcomes in lactating dairy cows consuming excessive energy, and receiving in a 2×2 factorial arrangement design: (1) concentrate based on ground corn (CRN; n=13) or citrus pulp (PLP; n=13), and (2) supplemented (n=14) or not (n=12) with 2.5 g/day of chromium (Cr)-propionate. During the experiment (day 0 to 182), 26 multiparous, non-pregnant, lactating Gir×Holstein cows (initial days in milk=80±2) were offered corn silage for ad libitum consumption, and individually received concentrate formulated to allow diets to provide 160% of their daily requirements of net energy for lactation...
March 2017: Animal: An International Journal of Animal Bioscience
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