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embryo in vitro production

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https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#1
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28518112/functional-manipulation-of-maternal-gene-products-using-in-vitro-oocyte-maturation-in-zebrafish
#2
Elaine L Welch, Celeste C Eno, Sreelaja Nair, Robin E Lindeman, Francisco Pelegri
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into the developing oocyte. Because these events rely on maternal products which typically act very soon after fertilization-that preexist inside the egg, standard approaches for expression and functional reduction involving the injection of reagents into the fertilized egg are typically ineffective. Instead, such manipulations must be performed during oogenesis, prior to or during the accumulation of maternal products...
April 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518085/analysis-of-chromosome-segregation-histone-acetylation-and-spindle-morphology-in-horse-oocytes
#3
Federica Franciosi, Irene Tessaro, Rozenn Dalbies-Tran, Cecile Douet, Fabrice Reigner, Stefan Deleuze, Pascal Papillier, Ileana Miclea, Valentina Lodde, Alberto M Luciano, Ghylene Goudet
The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513460/the-role-of-oxidative-stress-in-female-infertility-and-in-vitro-fertilization
#4
Joanna Wojsiat, Jerzy Korczyński, Marta Borowiecka, Halina Małgorzata Żbikowska
Infertility problem involves many couples of reproductive age. It has been estimated that in Poland 0.7-1.0 million pairs require treatment, while for more than half of them assisted reproduction is the only recommended and effective method. Infertility affects 13 to 15% of the world's population. A major concern is the age-related decline in female fertility even more that often a decision about pregnancy is taken at later age. Recent studies show that increased production of reactive oxygen species is an important factor in etiopathogenesis of pregnancy and affects female reproduction...
May 9, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28506817/in-vitro-cyp-mediated-drug-metabolism-in-the-zebrafish-embryo-using-human-reference-compounds
#5
Moayad Saad, An Matheeussen, Sebastiaan Bijttebier, Evy Verbueken, Casper Pype, Christophe Casteleyn, Chris Van Ginneken, Sandra Apers, Louis Maes, Paul Cos, Steven Van Cruchten
The increasing use of zebrafish embryos as an alternative model for toxicological and pharmacological studies necessitates a better understanding of xenobiotic biotransformation in this species. As cytochrome P450 enzymes (CYPs) play an essential role in this process, in vitro drug metabolism of four human CYP-specific substrates, i.e. dextromethorphan (DXM), diclofenac (DIC), testosterone (TST) and midazolam (MDZ) was investigated in adult male and female zebrafish, and in zebrafish embryos and larvae up to 120hours post-fertilization...
May 12, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28497913/role-of-antioxidants-in-assisted-reproductive-techniques
#6
REVIEW
Ashok Agarwal, Ahmad Majzoub
Oxidative stress (OS) has been recognized as a significant cause of suboptimal assisted reproductive outcome. Many of the sperm preparation and manipulation procedures that are necessary in the in vitro environment can result in excessive production of reactive oxygen species (ROS) thereby exposing the gametes and growing embryos to significant oxidative damage. Antioxidants have long been utilized in the management of male subfertility as they can counterbalance the elevated levels of ROS inducing a high state of OS...
April 30, 2017: World Journal of Men's Health
https://www.readbyqxmd.com/read/28496018/effects-of-heat-stress-on-bovine-preimplantation-embryos-produced-in-vitro
#7
Miki Sakatani
Summer heat stress decreases the pregnancy rate in cattle and has been thought to be associated with the early embryonic death caused by the elevation of maternal body temperature. In vitro cultures have been widely used for the evaluation of effects of heat stress on oocytes, fertilization, preimplantation, and embryonic development. Susceptibility to heat stress is present in developmental stages from oocytes to cleavage-stage (before embryonic gene activation, EGA) embryos, leading to a consequent decrease in developmental competence...
May 12, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28493788/development-of-in-vitro-embryo-production-system-using-collagen-matrix-gel-attached-vascular-endothelial-growth-factor-derived-from-interleukin-1-beta-treated-porcine-endometrial-tissue
#8
Hye-In Han, Sang-Hee Lee, Choon-Keun Park
The aim of this study was to establish an embryo culture system using collagen gel attached with vascular endothelial growth factor (VEGF) derived from interleukin-1 beta (IL-1β)-treated endometrial tissues from pigs. Endometria were separated from the porcine uterus at the follicular phase of the estrous cycle and were cultured with IL-1β. The collagen gels co-incubated with IL-1β-treated endometria (C, without endometrial tissue; CE, with endometrial tissue; and CEI, IL-1β-treated endometrial tissue) were used for embryo culture...
May 11, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28479126/low-serum-concentration-in-bovine-embryo-culture-enhances-early-blastocyst-rates-on-day-6-with-quality-traits-in-the-expanded-blastocyst-stage-similar-to-bsa-cultured-embryos
#9
A Murillo, M Muñoz, D Martín-González, S Carrocera, A Martínez-Nistal, E Gómez
In bovine, single in vitro embryo culture in protein-free medium from Day-6 to Day-7 leads to expanded blastocyst (XB) with improved pregnancy and birth rates after cryopreservation. Under these conditions, early blastocysts (EB) progress to the XB stage at higher rates than morulae (M). However, embryo production with BSA in culture prior to Day-6 leads to low EB rates. We investigated whether a very low FCS concentration (0.1%) in culture from Day-1 to Day-6 would improve EB rates and, subsequently, increase XB rates on Day-7 after single culture in protein-free medium...
May 4, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28475125/melatonin-promotes-the-in-vitro-development-of-microinjected-pronuclear-mouse-embryos-via-its-anti-oxidative-and-anti-apoptotic-effects
#10
Xiuzhi Tian, Feng Wang, Lu Zhang, Pengyun Ji, Jing Wang, Dongying Lv, Guangdong Li, Menglong Chai, Zhengxing Lian, Guoshi Liu
CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) combined with pronuclear microinjection has become the most effective method for producing transgenic animals. However, the relatively low embryo developmental rate limits its application. In the current study, it was observed that 10(-7) M melatonin is considered an optimum concentration and significantly promoted the in vitro development of murine microinjected pronuclear embryos, as indicated by the increased blastocyst rate, hatching blastocyst rate and blastocyst cell number...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28463020/treatment-of-donor-cells-and-reconstructed-embryos-with-a-combination-of-trichostatin-a-and-5-aza-2-deoxycytidine-improves-the-developmental-competence-and-quality-of-buffalo-embryos-produced-by-handmade-cloning-and-alters-their-epigenetic-status-and-gene-expression
#11
Monika Saini, Naresh L Selokar, Himanshu Agrawal, Suresh Kumar Singla, Manmohan Singh Chauhan, Radheysham S Manik, Prabhat Palta
The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine (5-aza-dC), an inhibitor of DNA methyltransferases, are widely used for altering the epigenetic status of cloned embryos. We optimized the doses of these epigenetic modifiers for production of buffalo embryos by handmade cloning and examined whether combined treatment with these epigenetic modifiers offered any advantage over treatment with the individual epigenetic modifier...
May 2, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28461734/auxin-and-nutritional-stress-coupled-somatic-embryogenesis-in-oldenlandia-umbellata-l
#12
S R Saranya Krishnan, E A Siril
Somatic embryos were induced from internodal segment derived callus of Oldenlandia umbellata L., in MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). Initially calli were developed from internodes of microshoots inoculated in 2.5 µM NAA supplemented medium. Then calli were transferred to 2,4-D added medium for somatic embryogenesis. Nutritional stress coupled with higher concentration of 2,4-D triggered somatic embryogenesis. Nutritional stress was induced by culturing callus in a fixed amount of medium for a period up to 20 weeks without any external supply of nutrients...
April 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28460003/evidence-for-cb2-receptor-involvement-in-lps-induced-reduction-of-camp-intracellular-levels-in-uterine-explants-from-pregnant-mice-pathophysiological-implications
#13
Ana Inés Salazar, Alejandro Carozzo, Fernando Correa, Carlos Davio, Ana María Franchi
STUDY QUESTION: What is the role of the endocannabinoid system (eCS) on the lipopolysaccharide (LPS) effects on uterine explants from 7-day pregnant mice in a murine model of endotoxin-induced miscarriage? SUMMARY ANSWER: We found evidence for cannabinoid receptor type2 (CB2) involvement in LPS-induced increased prostaglandin-F2α (PGF2α) synthesis and diminished cyclic adenosine monophosphate (cAMP) intracellular content in uterine explants from early pregnant mice...
April 28, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28456408/postnatal-phenotype-of-dairy-cows-is-altered-by-in-vitro-embryo-production-using-reverse-x-sorted-semen
#14
Luiz G B Siqueira, Serdal Dikmen, M Sofia Ortega, Peter J Hansen
Abnormal fetuses, neonates, and adult offspring derived by assisted reproductive technologies have been reported in humans and mice and have been associated with increased likelihood of certain adult diseases. To test the hypothesis that bovine females derived by assisted reproductive technologies have altered postnatal growth and adult function, a retrospective cohort study evaluated survival, growth, and production traits of offspring derived by in vitro embryo production (IVP) with conventional (IVP-conv) or reverse X-sorted semen (IVP-sexed), multiple ovulation and embryo transfer, and artificial insemination (AI) in a large dairy herd...
April 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28442051/cerium-oxide-nanoparticles-ceo2-nps-improve-the-developmental-competence-of-in-vitro-matured-prepubertal-ovine-oocytes
#15
F Ariu, L Bogliolo, A Pinna, L Malfatti, P Innocenzi, L Falchi, D Bebbere, S Ledda
The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO2 NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeO2NPs (0, 44, 88 or 220µg mL(-1)). Matured oocytes were fertilised in vitro and zygotes were cultured for 7 days. The results demonstrated that CeO2NPs were internalised in the cumulus cells and not in the oocyte...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28436137/insights-into-in-vitro-spermatogenesis-in-mammals-past-present-future
#16
REVIEW
Amir Fattahi, Zeinab Latifi, Tohid Ghasemnejad, Hamid Reza Nejabati, Mohammad Nouri
Considering the self-renewal and differentiation ability of pluripotent stem cells, some studies have pointed out the possibility of stem cell-derived sperm production. Most studies that test this hypothesis have been conducted on rodents, with some promising results; however, studies on humans are progressing slowly, and have encountered technical and ethical hurdles. Established methods to differentiate stem cells - including embryoid bodies, co-culturing, and various feeder cells - may provide a niche that is similar to in vivo conditions and resolve epigenetic abnormalities, but a gonadal-like three-dimensional structure is still required to produce germ cells with the correct imprinting...
April 24, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28432708/in-vitro-production-of-porcine-zygotes-using-intracytoplasmic-injection-of-vitrified-sperm
#17
C C Arraztoa, C Baca Castex, G M Alvarez, P D Cetica, D M Neild
The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO2 and tri-gas. A group of porcine oocytes maturated in vitro were injected with vitrified-warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test...
April 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28428264/cb1-cannabinoid-receptor-drives-oocyte-maturation-and-embryo-development-via-pi3k-akt-and-mapk-pathways
#18
Angela Patricia López-Cardona, Serafín Pérez-Cerezales, Raúl Fernández-González, Ricardo Laguna-Barraza, Eva Pericuesta, Naiara Agirregoitia, Alfonso Gutiérrez-Adán, Ekaitz Agirregoitia
Endocannabinoids have been recognized as mediators of practically all reproductive events in mammals. However, little is known about the role of this system in oocyte maturation. In a mouse model, we observed that activation of the cannabinoid receptor (CB)1 during in vitro oocyte maturation modulated the phosphorylation status of Akt and ERK1/2 and enhanced the subsequent embryo production. In the absence of the CB1 receptor, in vivo oocyte maturation was impaired and embryo development delayed. The CB2 receptor was unable to rescue these effects...
April 20, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28420480/comparative-analysis-of-granulosa-cell-gene-expression-in-association-with-oocyte-competence-in-fsh-stimulated-holstein-cows
#19
David A Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Marc-André Sirard
Ovarian stimulation with exogenous FSH followed by FSH withdrawal or 'coasting' is an effective means of increasing the number of oocytes obtainable for the in vitro production of cattle embryos. However, the quality of the oocytes thus obtained varies considerably from one cow to the next. The aim of the present study was to gain a better understanding of the follicular conditions associated with low oocyte developmental competence. Granulosa cells from 94 Holstein cows in a commercial embryo production facility were collected following ovarian stimulation and coasting...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28412991/inbreeding-effects-on-in-vitro-embryo-production-traits-in-guzer%C3%A3-cattle
#20
B C Perez, J C C Balieiro, R V Ventura, F A T Bruneli, M G C D Peixoto
Inbreeding has been associated with the impairment of reproductive performance in many cattle breeds. Although the usage of reproductive biotechnologies has been increasing in bovine populations, not much attention has been given to the impact of inbreeding over cow's performance on artificial reproduction. The objective of this study was to estimate the impact of inbreeding on in vitro embryo production in a Guzerá breed population. The inbreeding coefficient (F), calculated as half of the co-ancestry of the individual's parents, was used as an estimate of inbreeding...
April 17, 2017: Animal: An International Journal of Animal Bioscience
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