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https://www.readbyqxmd.com/read/28809025/isolation-and-characterization-of-chicken-primordial-germ-cells-and-their-application-in-transgenesis
#1
Jae Yong Han, Bo Ram Lee
Primordial germ cells (PGCs), precursors of functional gametes, offer great promise for the use of genetic resources and transgenesis in chickens. PGCs can be isolated from the developing embryo at diverse early stages and are subsequently expandable in vitro. In vitro proliferating chicken PGCs can facilitate the production of efficient germline chimeras and transgenic chickens. Here, we describe methods to isolate and characterize PGCs from chicken embryos and their application to transgenesis.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28805606/detection-of-sea-type-%C3%AE-thalassemia-in-embryo-biopsies-by-digital-pcr
#2
Ta-Hsien Lee, Ya-Chiung Hsu, Chia Lin Chang
OBJECTIVE: Accurate and efficient pre-implantation genetic diagnosis (PGD) based on the analysis of single or oligo-cells is needed for timely identification of embryos that are affected by deleterious genetic traits in in vitro fertilization (IVF) clinics. Polymerase chain reaction (PCR) is the backbone of modern genetic diagnoses, and a spectrum of PCR-based techniques have been used to detect various thalassemia mutations in prenatal diagnosis (PND) and PGD. Among thalassemias, SEA-type α-thalassemia is the most common variety found in Asia, and can lead to Bart's hydrops fetalis and serious maternal complications...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28799183/utilization-of-porcine-in%C3%A2-vitro-produced-parthenogenetic-embryos-for-co-transfer-with-vitrified-and-warmed-embryos
#3
Yoshiyuki Nakamura, Kazuhiro Kikuchi
The present study was conducted to evaluate the possibility of using in vitro-produced parthenogenetic (PA) embryos for co-transfer with morulae that had been collected in vivo and cryopreserved. The proportion of PA blastocysts (20.5%) was higher than that of their in vitro fertilization (IVF) counterparts (16.6%). Although there were no differences in morphology or diameter between the two groups, the number of cells in early PA blastocysts after in vitro culture for 6 days was lower than for IVF blastocysts (25...
August 10, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28789864/ethanol-exposure-leads-to-disorder-of-blood-island-formation-in-early-chick-embryo
#4
Guang Wang, Bin-Zhen Chen, Chao-Jie Wang, Jing Zhang, Lin-Rui Gao, Manli Chuai, Yongping Bao, Xuesong Yang
Ethanol's effect on embryonic vasculogenesis and its underlying mechanism is obscure. Using VE-cadherin in situ hybridization, we found blood islands formation was inhibited in area opaca, but abnormal VE-cadherin(+) cells were seen in area pellucida. We hypothesise ethanol may affect blood island progenitor cell migration and differentiation. DiI and in vitro experiments revealed ethanol inhibited cell migration, Quantitative PCR analysis revealed that ethanol exposure enhanced cell differentiation in area pellucida of HH5 chick embryos and repressed cell differentiation in area pellucida of HH8 chick embryos...
August 5, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28777818/gluc-het-a-complementary-chick-embryo-model-for-the-characterization-of-antidiabetic-compounds
#5
Renate Haselgrübler, Flora Stübl, Katja Essl, Marcus Iken, Klaus Schröder, Julian Weghuber
Insulin resistance and β cell failure are the main causes of elevated blood glucose levels in Type 2 diabetes mellitus (T2DM), a complex and multifactorial metabolic disease. Several medications to treat or reduce the symptoms of T2DM are used, including the injection of insulin and the application of insulin sensitizing or glucose production reducing drugs. Furthermore, the use of phytochemicals has attracted increasing attention for the therapy and prevention of T2DM. In order to identify and characterize antidiabetic compounds, efficient test systems are required...
2017: PloS One
https://www.readbyqxmd.com/read/28776828/the-quality-after-culture-in%C3%A2-vitro-or-in%C3%A2-vivo-of-porcine-oocytes-matured-and-fertilized-in%C3%A2-vitro-and-their-ability-to-develop-to-term
#6
Yoshiyuki Nakamura, Sigeyuki Tajima, Kazuhiro Kikuchi
The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo. We examined the quality of in vitro-matured and fertilized (IVM/IVF) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM/IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) ('ET-vivo' embryos), or cultured in vitro for 5 or 6 days ('IVC' embryos)...
August 4, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28776545/endometriosis-fertility-index-for-predicting-pregnancy-after-endometriosis-surgery
#7
Xin Li, Cheng Zeng, Ying-Fang Zhou, Hui-Xia Yang, Jing Shang, Sai-Nan Zhu, Qing Xue
BACKGROUND: The endometriosis fertility index (EFI) has a predictive value for pregnancy after surgery. In vitro fertilization and embryo transfer (IVF-ET) is a good treatment to infertility. This study aimed to provide external validation of EFI, assess the factors affecting the ability of EFI to predict cumulative spontaneous pregnancy rates (PRs), and propose reasonable advice for treatment by evaluating the effect of infertility management combining surgery and IVF-ET. METHODS: This retrospective study enrolled 345 endometriosis-related infertile women after laparoscopic surgery from January 2012 to January 2016...
August 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28776456/the-role-of-the-endoplasmic-reticulum-stress-response-following-cerebral-ischemia
#8
Gina Hadley, Ain A Neuhaus, Yvonne Couch, Daniel J Beard, Bryan A Adriaanse, Kostas Vekrellis, Gabriele C DeLuca, Michalis Papadakis, Brad A Sutherland, Alastair M Buchan
Background Cornu ammonis 3 (CA3) hippocampal neurons are resistant to global ischemia, whereas cornu ammonis (CA1) 1 neurons are vulnerable. Hamartin expression in CA3 neurons mediates this endogenous resistance via productive autophagy. Neurons lacking hamartin demonstrate exacerbated endoplasmic reticulum stress and increased cell death. We investigated endoplasmic reticulum stress responses in CA1 and CA3 regions following global cerebral ischemia, and whether pharmacological modulation of endoplasmic reticulum stress or autophagy altered neuronal viability ...
January 1, 2017: International Journal of Stroke: Official Journal of the International Stroke Society
https://www.readbyqxmd.com/read/28774621/zebrafish-embryo-toxicity-of-15-chlorinated-brominated-and-iodinated-disinfection-by-products
#9
David Hanigan, Lisa Truong, Michael Simonich, Robert Tanguay, Paul Westerhoff
Disinfection to protect human health occurs at drinking water and wastewater facilities through application of non-selective oxidants including chlorine. Oxidants also transform organic material and form disinfection by-products (DBPs), many of which are halogenated and cyto- and genotoxic. Only a handful of assays have been used to compare DBP toxicity, and researchers are unsure which DBP(s) drive the increased cancer risk associated with drinking chlorinated water. The most extensive data set employs an in vitro model cell, Chinese hamster ovary cells...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28764817/the-rpmi-1640-vitamin-mixture-promotes-bovine-blastocyst-development-in-vitro-and-downregulates-gene-expression-of-txnip-with-epigenetic-modification-of-associated-histones
#10
S Ikeda, M Sugimoto, S Kume
Diverse environmental conditions surrounding preimplantation embryos, including available nutrients, affect their metabolism and development in both short- and long-term manner. Thioredoxin-interacting protein (TXNIP) is a possible marker for preimplantation stress that is implicated in in vitro fertilization- (IVF) induced long-term DOHaD effects. B vitamins, as participants in one-carbon metabolism, may affect preimplantation embryos by epigenetic alterations of metabolically and developmentally important genes...
August 2, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28763725/peroxidized-mineral-oil-increases-the-oxidant-status-of-culture-media-and-inhibits-in%C3%A2-vitro-porcine-embryo-development
#11
C A Martinez, A Nohalez, J J Ceron, C P Rubio, J Roca, C Cuello, H Rodriguez-Martinez, E A Martinez, M A Gil
The use of oils with undetected alterations is a long-recognized problem for in vitro embryo production systems. Since peroxides in oils have been associated with reduced embryo production outcomes, our goals were (1) to evaluate the effects of a batch of mineral oil (MO) that was suspected to be altered on the in vitro production of pig embryos and (2) to determine oil peroxide values throughout culture and the transfer of oxidant agents from oil to culture media. Sunflower oil, which has a completely different chemical composition than MO but a higher oxidative status, and unaltered MO were used as controls...
July 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28756654/adverse-effect-of-superovulation-treatment-on-maturation-function-and-ultrastructural-integrity-of-murine-oocytes
#12
Myungook Lee, Jong Il Ahn, Ah Ran Lee, Dong Woo Ko, Woo Sub Yang, Gene Lee, Ji Yeon Ahn, Jeong Mook Lim
Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated...
July 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28755340/desiccation-enhanced-maturation-and-germination-of-date-palm-somatic-embryos-derived-from-cell-suspension-culture
#13
Nazim Boufis, Khayreddine Titouh, Lakhdar Khelifi
In vitro plant regeneration via somatic embryogenesis is a powerful tool for rapid, large-scale production of healthy true-to-type plants. This approach is suitable to preserve existing natural genetic variability and propagation of variability generated from genetic improvement programs, including crossing, somaclonal variation, mutagenesis, and somatic hybridization. This chapter outlines a simplified protocol for date palm regeneration via somatic embryogenesis induced in cell suspension cultures. In this protocol, culture medium composition is manipulated, including plant growth regulators and solid (addition of agar) and liquid media to achieve reduction of production cycle of somatic embryogenesis, which increases the multiplication rate of embryogenic callus and improves the quantity and quality of somatic embryos...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755215/encapsulation-of-date-palm-somatic-embryos-synthetic-seeds
#14
Shawky A Bekheet
Synthetic seed or encapsulated somatic embryos may be used for propagation, storage, and exchange of plant germplasm and have many diverse applications in date palm cultivation. They have advantages over conventional use of offshoot material for germplasm propagation, maintenance, exchange, and transportation. This chapter describes a protocol for date palm synthetic seed production by encapsulation of somatic embryos with sodium alginate. Among three concentrations used, 3% sodium alginate followed by dropping into 2...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28755146/interaction-of-different-forms-of-graphene-with-chicken-embryo-red-blood-cells
#15
Sławomir Jaworski, Mateusz Hinzmann, Ewa Sawosz, Marta Grodzik, Marta Kutwin, Mateusz Wierzbicki, Barbara Strojny, Krishna P Vadalasetty, Ludwika Lipińska, André Chwalibog
With the rapid development of graphene synthesis and functionalization approaches, graphene and its related derivatives have shown great potential in many applications in material science, including biomedical applications. Several in vitro and in vivo studies clearly showed no definitive risks, while others have indicated that graphene might become health hazards. In this study, we explore the biocompatibility of graphene-related materials with chicken embryo red blood cells (RBC). The hemolysis assay was employed to evaluate the in vitro blood compatibility of reduced graphene, graphene oxide, and reduced graphene oxide, because these materials have recently been used for biomedical applications, including injectable graphene-related particles...
July 28, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28754923/tauroursodeoxycholic-acid-enhances-the-development-of-porcine-embryos-derived-from-in-vitro-matured-oocytes-and-evaporatively-dried-spermatozoa
#16
Xiao-Xia Li, Yun-Fei Diao, Hai-Jun Wei, Shi-Yong Wang, Xin-Yan Cao, Yu-Fei Zhang, Tong Chang, Dan-Li Li, Min Kyu Kim, Baozeng Xu
Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28744919/differential-expression-of-insulin-like-growth-factor-family-members-in-immature-cumulus-oocyte-complexes-from-dairy-cows-with-different-genotypes
#17
A C Lopes, M P Palhão, Cac Fernandes, M J Sudano, Acs Castilho, E S Caixeta
It has been evident the improvement of in vitro embryo production (IVEP) in dairy cows. Nevertheless, it is known that differences in the number and quality of oocytes between taurine and zebu females impact the efficiency and economic viability of IVEP. As the insulin-like growth factor (IGF) system is related to follicular and oocyte development, we aimed to quantify mRNA abundance of IGF system members and pregnancy-associated plasma protein-A (PAPPA) in the cumulus-oocyte complexes (COCs) of Gir, 1/2 Holstein × 1/2 Gir and Holstein cows...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28735601/impact-of-equine-assisted-reproductive-technologies-standard-embryo-transfer-or-intracytoplasmic-sperm-injection-icsi-with-in-vitro-culture-and-embryo-transfer-on-placenta-and-foal-morphometry-and-placental-gene-expression
#18
Orlando A Valenzuela, Anne Couturier-Tarrade, Young-Ho Choi, Marie-Christine Aubrière, Justin Ritthaler, Pascale Chavatte-Palmer, Katrin Hinrichs
Assisted reproductive technologies (ARTs) such as intracytoplasmic sperm injection (ICSI), in vitro embryo culture and embryo transfer (ET) may be associated with alterations in fetal and placental development. In horses, ET has been used for decades. More recently, in vitro embryo production by ICSI and in vitro culture, followed by embryo transfer (ICSI-C) has become an accepted method for clinical foal production. However, no information is available on the effects of ICSI-C or even of standard ET itself on placental and neonatal parameters in horses...
July 24, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28735105/inherent-inferior-quality-of-follicular-fluid-in-repeat-breeder-heifers-as-evidenced-by-low-rates-of-in%C3%A2-vitro-production-of-bovine-embryos
#19
M Kafi, M Azari, O Chashnigir, S Gharibzadeh, Z Aghabozorgi, A Asaadi, M R Divar
The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0...
July 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28727799/atlantic-salmon-cardiac-primary-cultures-an-in-vitro-model-to-study-viral-host-pathogen-interactions-and-pathogenesis
#20
Patricia A Noguera, Bianka Grunow, Matthias Klinger, Katherine Lester, Bertrand Collet, Jorge Del-Pozo
Development of Salmon Cardiac Primary Cultures (SCPCs) from Atlantic salmon pre-hatch embryos and their application as in vitro model for cardiotropic viral infection research are described. Producing SCPCs requires plating of trypsin dissociated embryos with subsequent targeted harvest from 24h up to 3 weeks, of relevant tissues after visual identification. SCPCs are then transferred individually to chambered wells for culture in isolation, with incubation at 15-22°. SCPCs production efficiency was not influenced by embryo's origin (0...
2017: PloS One
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