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Zhengwen Zhou, Yue Zhang, Luning Lin, Jianmei Zhou
Apigenin, a flavonoid with multiple physiological and pharmacological activities, is associated with the prevention of cardiovascular diseases. The present study aimed to examine the roles and mechanisms of apigenin in the apoptosis of H9C2 rat cardiomyocytes, which were subjected to myocardial ischemia‑reperfusion (MI/R) injury. Cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cellular apoptosis were evaluated using cell counting kit‑8 assays and flow cytometry...
May 31, 2018: Molecular Medicine Reports
Qing-Hui Wen, Lang-Hong Wang, Xin-An Zeng, De-Bao Niu, Man-Sheng Wang
In this work, the hydroxyl-related differences of binding properties and inhibitory activities of dietary flavonoids, namely chrysin, baicalein and apigenin against purine nucleoside phosphorylase (PNP) were investigated. It was found that the hydroxylation on position C4' of chrysin (→apigenin) mildly decreased the binding affinities for PNP, whereas on the position C6 of chrysin (→baicalein) significantly increased binding affinities. Comparatively, the hydroxylation on position C4' and C6 greatly improved their PNP inhibitory effects...
June 9, 2018: International Journal of Biological Macromolecules
Antonella Lascala, Concetta Martino, Maddalena Parafati, Raffaele Salerno, Manuela Oliverio, Daniela Pellegrino, Vincenzo Mollace, Elzbieta Janda
Autophagy dysfunction has been implicated in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Natural compounds present in bergamot polyphenol fraction (BPF) prevent NAFLD and induce autophagy in rat livers. Here, we employed HepG2 cells expressing DsRed-LC3-GFP, a highly sensitive model system to screen for proautophagic compounds present in BPF. BPF induced autophagy in a time- and dose-dependent fashion and the effect was amplified in cells loaded with palmitic acid. Autophagy was mediated by the hydrophobic fraction of acid-hydrolyzed BPF (A-BPF), containing six flavanone and flavone aglycones as identified by liquid chromatography-high-resolution mass spectrometry...
April 26, 2018: Journal of Nutritional Biochemistry
Kunping Li, Zhuoru He, Xinqiuyue Wang, Miguel Pineda, Runbao Chen, Haiqi Liu, Kaiting Ma, Huanjia Shen, Chunhui Wu, Ningtin Huang, Tianling Pan, Yun Liu, Jiao Guo
Acute lung injury (ALI) and its more severe form acute respiratory distress syndrome (ARDS) are life-threatening conditions with high morbility and mortality, underscoring the urgent need for novel treatments. Leaves of the medicinal herb Microcos paniculata have been traditionally used for treating upper airway infections, by virtue of its content of flavonoids such as apigenin C-glycosides (ACGs). C-glycosides have been shown to exert strong anti-inflammatory properties, although their mechanism of action remains unknown...
June 8, 2018: Free Radical Biology & Medicine
Cairui Lu, Cong Li, Bang Chen, Yehua Shen
This study aims at identifying the composition of polyphenols present in Amygdalus pedunculata Pall seed coat (APSC), and characterizing their antioxidant, antibacterial, and anticancer activities. The polyphenols from APSC were composed of 32 compounds. The compounds with important biological activities included apigenin 7-O-glucoside (the main component; 34.53 mg/100 g), quercitrin (23.43 mg/100 g), kaempferol (10.28 mg/100 g), naringenin (6.27 mg/100 g), cyanidin 3-rutinoside (5.76 mg/100 g), cyanidin 3-O-galactoside (5...
November 1, 2018: Food Chemistry
Joana M Pereira, Vanessa Peixoto, Alexandra Teixeira, Diana Sousa, Lillian Barros, Isabel C F R Ferreira, M Helena Vasconcelos
The cell growth inhibitory activity of the hydroethanolic extract of Achillea millefolium was studied in human tumor cell lines (NCI-H460 and HCT-15) and its mechanism of action was investigated. The GI50 concentration was determined with the sulforhodamine B assay and cell cycle and apoptosis were analyzed by flow cytometry following incubation with PI or Annexin V FITC/PI, respectively. The expression levels of proteins involved in cell cycle and apoptosis were analyzed by Western blot. The extracts were characterized regarding their phenolic composition by LC-DAD-ESI/MS...
June 5, 2018: Food and Chemical Toxicology
Weixin Wang, Shuwei Zhang, Lishuang Lv, Shengmin Sang
Thearubigins (TRs) are the major components of black tea, which are formed during the fermentation reactions. Although anti-inflammatory and anti-cancer activities of TRs have been reported, the prepared TRs according to the literature methods still contain many floating peaks. It is puzzling whether the observed activities are from TRs or these floating peaks. Thus, it is urgent to develop a method to prepare pure TRs and redefine them. In the present study, we developed a new method, the combination of caffeine precipitation and Sephadex LH-20 column chromatography, to prepare pure TRs...
May 28, 2018: Journal of Chromatography. A
Hala Soltana, Mirko De Rosso, Houda Lazreg, Antonio Dalla Vedova, Mohamed Hammami, Riccardo Flamini
Flavonoids are compounds characterized by antioxidant activity and their intake in the human diet is considered useful for health and nutrition. Non-anthocyanic flavonoids in four different types of Tunisian figs belonging to the smyrna-type Ficus carica varieties known as Kholi, Tchich Asal, Himri and Bidhi were studied by liquid chromatography/high-resolution mass spectrometry UHPLC-QTOF. Twenty-two compounds belonging to the classes of flavanones (naringenin and eriodictyol), flavones (3 apigenin and 5 luteolin derivatives) and flavonols (2 kaempferol and 7 quercetin derivatives), were identified...
June 2, 2018: Journal of Mass Spectrometry: JMS
Seung Hwan Hwang, Zhiqiang Wang, Yanymee N Guillen Quispe, Soon Sung Lim, Jae Myung Yu
The inhibitory activities of Matricaria recutita L. 70% methanol extract were evaluated by isolating and testing 10 of its compounds on rat lens aldose reductase (RLAR), advanced glycation end products (AGEs), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Among these compounds, apigenin-7- O - β -D-glucoside, luteolin-7- O - β -D-glucoside, apigenin-7- O - β -D-glucuronide, luteolin-7- O - β -D-glucuronide, 3,5- O -di-caffeoylquinic acid, apigenin, and luteolin showed potent inhibition, and their IC50 values in RLAR were 4...
2018: Journal of Diabetes Research
Myung-Hee Lee, Ah-Ram Han, Mi Jang, Hyo-Kyoung Choi, Sung-Young Lee, Kyung-Tack Kim, Tae-Gyu Lim
Kaempferia parviflora (Krachaidum (KD)) is a traditional herbal medicine and has properties that are beneficial for human health. In the current study, we sought to investigate the anti-inflammatory properties of KD extract (KPE). In mouse skin tissue, UV light representing solar wavelengths (sUV) increased COX-2 expression, while treatment with KPE reduced this effect. The anti-inflammatory activity of KPE was confirmed in in vitro models. MAPK signaling pathways were activated by sUV irradiation, and this was also repressed in the presence of KPE treatment...
2018: Oxidative Medicine and Cellular Longevity
Dong Yang, Xiling Zhang, Wencun Zhang, Thamaraiselvan Rengarajan
Background: Colorectal cancer (CRC) is among highest prevailing cancers in the whole world, especially in western countries. For a diverse of reasons, patients prefer naturally occurring dietary substances over synthetic agents to prevent cancer. Vicenin-2 is largely available in a medicinal plant Ocimum sanctum and is an apigenin form, 6,8-di-C-glucoside, which has been reported to have a range of pharmacological values which includes antioxidant, hepatoprotective, anti-inflammatory and anti-cancer...
2018: Drug Design, Development and Therapy
Pallavi Sharma, Supriya Sharma, Damanpreet Singh
OBJECTIVES: Apigenin is the most common bioflavonoid known to be biologically active after systemic administration and show multiple pharmacological effects. The present study was designed to explore the role of apigenin in pentylenetetrazole (PTZ) kindling associated cognitive and behavioural impairments in the mouse model. METHODS: The animals were kindled by injecting a sub-convulsive dose of PTZ (35 mg/kg) on every alternate day, followed by 20 days treatment with apigenin at two different doses (10 and 20 mg/kg)...
May 30, 2018: Nutritional Neuroscience
Olubukola T Adenubi, Muna Ali Abdalla, Aroke S Ahmed, Emmanuel M Njoya, Lyndy J McGaw, Jacobus N Eloff, Vinny Naidoo
The menace caused by ticks and tick-borne diseases is a major limitation to the livestock industry in Africa. The high costs and non-availability of synthetic, chemical acaricides to resource-limited farmers, resistance of ticks to available acaricides and residue problems in meat and milk consumed by humans further complicate matters. The use of plant extracts as a possible source of new acaricides has received much interest in the last decade. In our endeavour to discover natural acaricidal compounds, tick toxicant bioassays were conducted and the chloroform fraction of Calpurnia aurea ethanol leaf extract had good acaricidal activity...
May 30, 2018: Experimental & Applied Acarology
Arman Zargaran, Afshin Borhani-Haghighi, Mohammad Salehi-Marzijarani, Pouya Faridi, Saeid Daneshamouz, Amir Azadi, Hossein Sadeghpour, Amirhossein Sakhteman, Abdolali Mohagheghzadeh
Phytotherapy is a source of finding new remedies for migraine. Traditional chamomile oil (chamomile extraction in sesame oil) is a formulation in Persian medicine (PM) for pain relief in migraine. An oleogel preparation of reformulated traditional chamomile oil was prepared and then standardized based on chamazulene (as a marker in essential oil) and apigenin via gas chromatography (GC) and high-performance liquid chromatography (HPLC) methods, respectively. A crossover double-blind clinical trial was performed with 100 patients...
May 28, 2018: Neurological Sciences
Sanchari Bhattacharya, Laboni Mondal, Biswajit Mukherjee, Lopamudra Dutta, Iman Ehsan, Mita C Debnath, Raghuvir H Gaonkar, Murari M Pal, Subrata Majumdar
Hepatocellular carcinoma (HCC) is one of the major causes of cancer related death globally. Apigenin, a dietary flavonoid, possesses anti-tumor activity against HCC cells in-vitro. Development, physicochemical characterization of apigenin loaded nanoparticles (ApNp), biodistribution pattern and pharmacokinetic parameters of apigenin upon intravenous administration of ApNp, and effect of ApNp treatment in rats with HCC were investigated. Apigenin loaded nanoparticles had a sustained drug release pattern and successfully reached the hepatic cancer cells in-vitro as well as in liver of carcinogenic animals...
May 23, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Stephen A Adefegha, Sunday I Oyeleye, Ganiyu Oboh
Background The seeds of African crocus (AC) (Curculigo pilosa) and wonderful kola (WK) (Buchholzia coriacea) are commonly used in folklore medicine in managing erectile dysfunction (ED) without the full understanding of the possible mechanism of actions. This study investigated and compared the effects of aqueous extracts from the seeds of AC and WK on arginase and acetylcholinesterase (AChE) activities and some pro-oxidant [FeSO4 and sodium nitroprusside (SNP)]-induced lipid peroxidation in rat penile homogenate in vitro...
May 23, 2018: Journal of Complementary & Integrative Medicine
Ali M El-Halawany, Hossam M Abdallah, Ahmed R Hamed, Hany Ezzat Khalil, Ameen M Almohammadi
BACKGROUND: There are increasing interests in natural compounds for cancer chemoprevention. Blocking agents represent an important class of chemopreventive compounds. They prevent carcinogens from undergoing metabolic activation and thereby suppressing their interaction with cellular macromolecular targets. METHODS: The effect of phenolic compounds isolated from Barleria cristata var. alba as chemopreventive agent was evaluated. The ethyl acetate fraction of B. cristata was subjected to different chromatographic techniques for isolation of its major phenolic compounds...
May 22, 2018: BMC Complementary and Alternative Medicine
Federico Ferreres, Nelson G M Gomes, Patrícia Valentão, David M Pereira, Angel Gil-Izquierdo, Luísa Araújo, Tânia C Silva, Paula B Andrade
The small tree Allophylus africanus, widespread in the African continent, has long been considered valuable, as noted by the number of reports on their multiple medicinal uses. With this work, we aimed to extend the current, and so far restricted, knowledge on the chemical composition of the plant, particularly as source of flavonoids, as well as to assess its potential anti-inflammatory properties. The chemical characterization of the aqueous extract obtained from the leaves allowed the identification and quantitation of 30 flavones, predominantly apigenin derivatives, but also four luteolin derivatives, while the stem bark extract was solely characterized by apigenin di-C-glycosides and mono-C-glycosides-O-glycosylated...
May 19, 2018: Food and Chemical Toxicology
Shreeya Ravisankar, Kebede Abegaz, Joseph M Awika
Reliable data on polyphenol in teff, an increasingly important food crop, is currently lacking. This study investigated the structural and quantitative profile of soluble and bound polyphenols in white and brown teff grown in Ethiopia and USA using LC-tandem quadrupole mass spectrometry. Thirty-four phenolic compounds (32 newly identified in teff), mostly flavones and phenolic acid derivatives, were characterized. Unusually high levels of flavones were present in both white (1398-2049 μg/g), and brown (1720-1847 μg/g) teff soluble fractions...
October 15, 2018: Food Chemistry
Kota Kera, Dennis D Fine, Daniel J Wherritt, Yoshiki Nagashima, Norimoto Shimada, Takeshi Ara, Yoshiyuki Ogata, Lloyd W Sumner, Hideyuki Suzuki
Introduction: Oxygen from carbon dioxide, water or molecular oxygen, depending on the responsible enzyme, can lead to a large variety of metabolites through chemical modification. Objectives: Pathway-specific labeling using isotopic molecular oxygen (18 O2 ) makes it possible to determine the origin of oxygen atoms in metabolites and the presence of biosynthetic enzymes (e.g., oxygenases). In this study, we established the basis of 18 O2 -metabolome analysis. Methods: 18 O2 labeled whole Medicago truncatula seedlings were prepared using 18 O2 -air and an economical sealed-glass bottle system...
2018: Metabolomics: Official Journal of the Metabolomic Society
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