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https://www.readbyqxmd.com/read/28719628/salt-tolerant-and-sensitive-alfalfa-medicago-sativa-cultivars-have-large-variations-in-defense-responses-to-the-lepidopteran-insect-spodoptera-litura-under-normal-and-salt-stress-condition
#1
Yunting Lei, Qing Liu, Christian Hettenhausen, Guoyan Cao, Qing Tan, Weiye Zhao, Honghui Lin, Jianqiang Wu
In nature, plants are often exposed to multiple stress factors at the same time. Yet, little is known about how plants modulate their physiology to counteract simultaneous abiotic and biotic stresses, such as soil salinity and insect herbivory. In this study, insect performance bioassays, phytohormone measurements, quantification of transcripts, and protein determination were employed to study the phenotypic variations of two alfalfa (Medicago sativa) cultivars in response to insect Spodoptera litura feeding under normal and salt stress condition...
2017: PloS One
https://www.readbyqxmd.com/read/28715254/effects-of-ethyl-%C3%AE-d-glucoside-on-human-dermal-fibroblasts
#2
Takayuki Bogaki, Keiichi Mitani, Yuki Oura, Kenji Ozeki
Ethyl α-d-glucoside (α-EG) is a glycoside present in sake, Japanese rice wine. Previous studies have reported that α-EG suppresses skin roughness after ultraviolet B irradiation, transepidermal water loss, and hepatic function disorder, and has a skin moisturizing effect. In this study, 0.48 μM of α-EG was found to increase the proliferation of normal human dermal fibroblasts (NHDF) by 121.0%, and the amount of collagen I produced by NHDF increased by 159.6% at an α-EG concentration of 0.048 μM, compared to those in cells cultured without α-EG...
July 17, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28713978/bone-marrow-mesenchymal-stem-cell%C3%A2-derived-extracellular-vesicles-improve-the-survival-of-transplanted-fat-grafts
#3
He Huang, Shaoqing Feng, Wenjie Zhang, Wei Li, Peng Xu, Xiangsheng Wang, Ai Ai
Autologous fat grafting is a promising surgical technique for soft tissue augmentation, reconstruction and rejuvenation. However, it is limited by the low survival rate of the transplanted fat, due to the slow revascularization of such grafts. Previous studies have demonstrated that bone marrow mesenchymal stem cell‑derived extracellular vesicles (BMSC‑EVs) are proangiogenic. The present study aimed to investigate whether BMSC‑EVs could improve the survival of transplanted fat grafts. Extracellular vesicles were isolated from the supernatant of cultured rat bone marrow mesenchymal stem cells, and characterized by flow cytometry and scanning electron microscopy...
July 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713973/a-dichloromethane-fraction-of-triticum-aestivum-sprouts-reduces-allergic-immune-response-through-inhibiting-th2-differentiation-in-ovalbumin%C3%A2-immunized-mice
#4
Hyeon-Hui Ki, Sung-Woo Hwang, Ji-Hyun Lee, Young-Ho Kim, Dae-Ki Kim, Young-Mi Lee
Triticum aestivum sprouts are small shoots that germinate from seeds and are consumed as a dietary supplement. The present study aimed to determine whether a dichloromethane fraction isolated from Triticum aestivum sprouts (TDF) suppressed the allergic immune response in ovalbumin (OVA)‑sensitized mice. In vivo experiments were performed by administering TDF or vehicle to mice during the sensitization and this was immediately followed by an intradermal injection of OVA into the ears. Splenocytes isolated from OVA‑sensitized mice were pre‑treated with TDF and re‑challenged with OVA for ex vivo evaluation...
July 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28708509/gdf8-activates-p38-mapk-signaling-during-porcine-oocyte-maturation-in%C3%A2-vitro
#5
Junchul David Yoon, Seon-Ung Hwang, Eunhye Kim, Minghui Jin, Soochong Kim, Sang-Hwan Hyun
Growth Differentiation Factor 8 (GDF8) is a member of the transforming growth factor-β (TGF-β) family and has been identified as a strong physiological regulator. This factor is expressed as a paracrine factor in mural granulosa cells. To investigate the effects of GDF8 on the in vitro maturation (IVM) of porcine oocytes, we assessed the quality of matured oocytes as well as the specific gene transcription and protein activation levels in oocytes and cumulus cells (CCs) after IVM and subsequent embryonic development after in vitro fertilization (IVF) and parthenogenetic activation (PA)...
June 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28701865/processed-panax-ginseng-sun-ginseng-inhibits-the-differentiation-and-proliferation-of-3t3-l1-preadipocytes-and-fat-accumulation-in-caenorhabditis-elegans
#6
Hyejin Lee, Jinhee Kim, Jun Yeon Park, Ki Sung Kang, Joeng Hill Park, Gwi Seo Hwang
BACKGROUND: Heat-processed ginseng, sun ginseng (SG), has been reported to have improved therapeutic properties compared with raw forms, such as increased antidiabetic, anti-inflammatory, and antihyperglycemic effects. The aim of this study was to investigate the antiobesity effects of SG through the suppression of cell differentiation and proliferation of mouse 3T3-L1 preadipocyte cells and the lipid accumulation in Caenorhabditis elegans. METHODS: To investigate the effect of SG on adipocyte differentiation, levels of stained intracellular lipid droplets were quantified by measuring the oil red O signal in the lipid extracts of cells on differentiation Day 7...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28698966/two-skin-cell-lines-from-wild-type-and-albino-japanese-flounder-paralichthys-olivaceus-establishment-characterization-virus-susceptibility-efficient-transfection-and-application-to-albinism-study
#7
Ruoqing Wang, Nianwei Zhang, Renkai Wang, Shengpeng Wang, Na Wang
In order to provide an applicable cell platform to study fish pathology and skin pigmentation, two cell lines derived from skin tissues of wild-type and albino Japanese flounder were established and named JFSK_wt and JFSK_alb, respectively. These two cell lines were cultured for 45 passages within approximately 300 days. JFSK_wt and JFSK_alb cells were maintained in Dulbecco's Modified Eagle's Medium and Ham's F-12 Nutrient Mixture (DMEM/F12) supplemented with antibiotics, fetal bovine serum (FBS), 2-mercaptoethanol (2-Me), N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), and basic fibroblast growth factor (bFGF)...
July 11, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28697461/direct-induction-of-functional-neuronal-cells-from-fibroblast-like-cells-derived-from-adult-human-retina
#8
Lili Hao, Zhen Xu, Hui Sun, Wu Luo, Youchen Yan, Jing Wang, Jingyi Guo, Yizhi Liu, Shuyi Chen
Obtaining and manipulating neuronal cells are critical for neural biology basic mechanism studies and translational applications. Recent advances in protocol development and mechanism dissections have made direct induction of neuronal cells from other somatic cells (iN) a promising strategy for such purposes. In this study, we established a protocol to expand a population of fibroblast-like cells from adult human retinal tissues, which can be reprogrammed into iNs by forced expression of neurogenic transcription factors...
June 29, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28693648/optimal-doses-of-egf-and-gdnf-act-as-biological-response-modifiers-to-improve-porcine-oocyte-maturation-and-quality
#9
Mehdi Vafaye Valleh, Nahid Karimi Zandi, Mikkel Aabech Rasmussen, Poul Hyttel
It is well documented that both epidermal growth factor (EGF) and glial cell line-derived neurotrophic factor (GDNF) are critical for porcine oocyte maturation, however, little information is known about their mechanism of action in vitro. To gain insight into the mechanisms of action of the optimum doses of EGF and GDNF on porcine oocyte maturation, porcine cumulus-oocyte complexes (COCs) were matured in defined porcine oocyte medium supplemented with EGF, GDNF or a combination of both at varying concentrations (0-100 ng/ml) for 44 h...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28693422/relationships-between-drought-heat-and-air-humidity-responses-revealed-by-transcriptome-metabolome-co-analysis
#10
Elisabeth Georgii, Ming Jin, Jin Zhao, Basem Kanawati, Philippe Schmitt-Kopplin, Andreas Albert, J Barbro Winkler, Anton R Schäffner
BACKGROUND: Elevated temperature and reduced water availability are frequently linked abiotic stresses that may provoke distinct as well as interacting molecular responses. Based on non-targeted metabolomic and transcriptomic measurements from Arabidopsis rosettes, this study aims at a systematic elucidation of relevant components in different drought and heat scenarios as well as relationships between molecular players of stress response. RESULTS: In combined drought-heat stress, the majority of single stress responses are maintained...
July 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28689061/%C3%AE-cryptoxanthin-ameliorates-metabolic-risk-factors-by-regulating-nf-%C3%AE%C2%BAb-and-nrf2-pathways-in-insulin-resistance-induced-by-high-fat-diet-in-rodents
#11
Kazim Sahin, Cemal Orhan, Fatih Akdemir, Mehmet Tuzcu, Nurhan Sahin, Ismet Yılmaz, Vijaya Juturu
The aim of this experiment was to determine the effects of β-cryptoxanthin (BCX) on the cardiometabolic health risk factors and NF-κB and Nrf2 pathway in insulin resistance induced by high-fat diet (HFD) in rodents. Twenty-eight Sprague-Dawley rats were allocated into four groups: (1) Control, rats fed a standard diet for 12 weeks; (2) BCX, rats fed a standard diet and supplemented with BCX (2.5 mg/kg BW) for 12 weeks; (3) HFD, rats fed a HFD for 12 weeks, (4) HFD + BCX, rats fed a HFD and supplemented with BCX for 12 weeks...
July 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28688659/indole-3-carbinol-is-a-potent-inhibitor-of-ischemia-reperfusion-induced-inflammation
#12
Emmanuel Ampofo, Nico Lachnitt, Jeannette Rudzitis-Auth, Beate M Schmitt, Michael D Menger, Matthias W Laschke
BACKGROUND: Ischemia-reperfusion (I/R) induces tissue inflammation, which is characterized by an increased leukocyte-endothelial cell interaction and leukocyte transmigration. These processes are mediated by the activation of the nuclear factor (NF)κB signaling pathway, resulting in an elevated expression of specific adhesion molecules. The phytochemical indole-3-carbinol (I3C) has been shown to exert anti-inflammatory effects by interfering with NFκB signal transduction. The aim of the present study was to investigate whether I3C is capable of counteracting the pathogenesis of I/R injury...
July 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28686580/upregulation-of-lyar-induces-neuroblastoma-cell-proliferation-and-survival
#13
Yuting Sun, Bernard Atmadibrata, Denise Yu, Matthew Wong, Bing Liu, Nicholas Ho, Dora Ling, Andrew E Tee, Jenny Wang, Imran N Mungrue, Pei Y Liu, Tao Liu
The N-Myc oncoprotein induces neuroblastoma by regulating gene transcription and consequently causing cell proliferation. Paradoxically, N-Myc is well known to induce apoptosis by upregulating pro-apoptosis genes, and it is not clear how N-Myc overexpressing neuroblastoma cells escape N-Myc-mediated apoptosis. The nuclear zinc finger protein LYAR has recently been shown to modulate gene expression by forming a protein complex with the protein arginine methyltransferase PRMT5. Here we showed that N-Myc upregulated LYAR gene expression by binding to its gene promoter...
July 7, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28680454/piperine-enhances-carbohydrate-fat-metabolism-in-skeletal-muscle-during-acute-exercise-in-mice
#14
Jisu Kim, Kang-Pa Lee, Dae-Won Lee, Kiwon Lim
BACKGROUND: Exercise promotes energy metabolism (e.g., metabolism of glucose and lipids) in skeletal muscles; however, reactive oxygen species are also generated during exercise. Various spices have been reported to have beneficial effects in sports medicine. Here, we investigated the effects of piperine, an active compound in black pepper, to determine its effects on metabolism during acute endurance exercise. METHODS: ICR mice (n = 18) were divided into three groups: nonexercise (CON), exercise (EX), and exercise with piperine (5 mg/kg) treatment (EP)...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28677764/serum-is-an-indispensable-factor-in-the-maintenance-of-the-biological-characteristics-of-sweat-gland-cells
#15
Qing Sun, Xiao-Mei Deng, Yun-Liang Wang, Yun-Fang Zhen, Fang Li, Rui-Hua Chen, Han-Si Liang, Feng Zhang, Ming-De Qin, Xue-Guang Zhang
The tolerance of sweat gland cells for in vitro amplification and subcultivation is low as they are somatic cells. The present study aimed to formulate an optimal medium for the culture of human eccrine sweat gland cells (HESGCs) and to establish a method for induction of HESGCs proliferation, whilst maintaining the characteristics of sweat gland cells. HESGCs cultured in sweat gland (SG):keratinocyte growth medium‑2 (KGM‑2) (1:1) medium had a higher proliferation rate and a stable morphology compared with cells cultured in SG and KGM‑2 medium only...
July 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28677743/yi-guan-jian-decoction-may-enhance-hepatic-differentiation-of-bone-marrow%C3%A2-derived-mesenchymal-stem-cells-via-sdf%C3%A2-1-in%C3%A2-vitro
#16
Lin-Lin Fu, Bing-Yao Pang, Ying Zhu, Ling Wang, Ai-Jing Leng, Hai-Long Chen
A previous study reported that Yi Guan Jian (YGJ) may increase the proliferation and differentiation of hepatic oval cells in a rat liver cirrhosis model. The aim of the present study was to investigate the effect and mechanism of action of YGJ on inducing hepatic differentiation in bone marrow‑derived mesenchymal stem cells (BM‑MSCs) via stromal‑cell derived factor‑1 (SDF‑1). Murine BM‑MSCs were isolated with whole bone marrow adherence, then identified by immunocytochemical staining and flow cytometry...
June 29, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28674000/genome-wide-screen-for-genes-involved-in-edna-release-during-biofilm-formation-by-staphylococcus-aureus
#17
Alicia S DeFrancesco, Nadezda Masloboeva, Adnan K Syed, Aaron DeLoughery, Niels Bradshaw, Gene-Wei Li, Michael S Gilmore, Suzanne Walker, Richard Losick
Staphylococcus aureus is a leading cause of both nosocomial and community-acquired infection. Biofilm formation at the site of infection reduces antimicrobial susceptibility and can lead to chronic infection. During biofilm formation, a subset of cells liberate cytoplasmic proteins and DNA, which are repurposed to form the extracellular matrix that binds the remaining cells together in large clusters. Using a strain that forms robust biofilms in vitro during growth under glucose supplementation, we carried out a genome-wide screen for genes involved in the release of extracellular DNA (eDNA)...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28659745/protective-effects-of-tea-polyphenols-on-exhaustive-exercise-induced-fatigue-inflammation-and-tissue-damage
#18
Lixia Liu, Xiuqin Wu, Bingchen Zhang, Wei Yang, Daliang Li, Yanqiu Dong, Yujiao Yin, Qi Chen
Background: The beneficial properties of tea polyphenols have been extensively studied; however, less attention has been paid to their effects, especially anti-inflammatory effect during exhaustive exercise. Objective: The present study assessed the potential protective effects of tea polyphenols against the fatigue, inflammation and tissue injury caused by an exhaustive exercise bout in rats. Design: Twenty-four healthy male rats were divided into three groups. Group C was a sedentary control group, Groups E+TP and Group E performed a single exhaustive swimming test; all groups had normal diets, but Group E+TP was supplemented with tea polyphenols...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28657734/anthocyanins-from-black-chokeberry-aronia-melanocarpa-elliot-delayed-aging-related-degenerative-changes-of-brain
#19
Jie Wei, Guokun Zhang, Xiao Zhang, Dexin Xu, Jun Gao, Jungang Fan, Zhiquan Zhou
Aging is the greatest risk factor for most neurodegenerative diseases, which is associated with decreasing cognitive function and significantly affectinglife quality in the elderly. Computational analysis suggested that 4 anthocyanins from chokeberry fruit increased Klotho (aging-suppressor) structural stability, so we hypothesized that chokeberry anthocyanins could anti-aging. To explore the effectsof anthocyanins treatment on brain aging, Mice treated with 15 or 30 mg/kg anthocyanins by gavage and injected D-galactose accelerated aging per day...
June 28, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28650445/a-distinct-function-of-the-retinoblastoma-protein-in-the-control-of-lipid-composition-identified-by-lipidomic-profiling
#20
H Muranaka, A Hayashi, K Minami, S Kitajima, S Kohno, Y Nishimoto, N Nagatani, M Suzuki, L A N Kulathunga, N Sasaki, N Okada, T Matsuzaka, H Shimano, H Tada, C Takahashi
Here, by combining lipidomics with transcriptome analysis, we demonstrate that Rb depletion in mouse embryonic fibroblastss induces significant alterations in their lipid composition. We discovered that Rb depletion induced increase in lysophosphatidylserine, diacylglycerol (DAG), fatty acid (FA), acylcarnitine, phosphatidylcholine (PC), arachidonoyl ethanolamine, and decrease in phosphatidylglycerol, monoacylglycerol, without change in total lipid per protein levels. Analysis of the acyl chain composition of DAG, PC and phosphatidylserine revealed increase of saturated and mono-unsaturated acyl chains with specific carbon chain length...
June 26, 2017: Oncogenesis
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