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Zhan-Feng Xia, Xiao-Xia Luo, Chuan-Xing Wan, Li-Li Zhang
A novel halophilic actinomycete strain, designated TRM 46074(T), was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074(T) was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose...
March 2017: International Journal of Systematic and Evolutionary Microbiology
Junwei Zhao, Linlin Shi, Wenchao Li, Jiabin Wang, Han Wang, Yuanyuan Tian, Wensheng Xiang, Xiangjing Wang
Two novel actinomycete isolates, designated strains NEAU-A4(T) and NEAU-A3, were isolated from rhizosphere soil of wheat (Triticumaestivum L.) and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the two strains coincided with those of the genus Streptomyces. The 16S rRNA gene sequence analysis showed that the two isolates exhibited 99.6 % 16S rRNA gene sequence similarity with each other and that they were most closely related to Streptomyces violaceorectus DSM 40279(T) (98...
November 16, 2017: International Journal of Systematic and Evolutionary Microbiology
V R Ranjitha, Ravishankar Rai Vittal
In the present study, the extracellular synthesis of Selenium nanoparticles (SeNPs) was carried out by using culture supernatant of Streptomyces griseoruber, an Actinomycetes member isolated from the soil. Bioreduction of SeNPs was confirmed by UV-Visible spectrophotometer that showed the peak at 575 nm. XRD pattern confirmed the non-crystalline or amorphous nature of the synthesised SeNPs. FTIR spectra revealed the possible functional group that is responsible for mediating the SeNPs formation. Size and distribution of the biosynthesised SeNPs were analysed by HR-TEM that showed the formation of particle size in the range of 100-250nm...
November 13, 2017: Pharmaceutical Nanotechnology
Niti Vanee, J Paul Brooks, Stephen S Fong
Actinomycetes have a long history of being the source of numerous valuable natural products and medicinals. To expedite product discovery and optimization of biochemical production, high-throughput technologies can now be used to screen the library of compounds present (or produced) at a given time in an organism. This not only facilitates chemical product screening, but also provides a comprehensive methodology to the study cellular metabolic networks to inform cellular engineering. Here, we present some of the first metabolomic data of the industrial cellulolytic actinomycete Thermobifida fusca generated using LC-MS/MS...
November 11, 2017: Metabolites
Wenchao Li, Junwei Zhao, Linlin Shi, Jiabin Wang, Han Wang, Xiangjing Wang, Wensheng Xiang
Two actinomycete strains, NEAU-C11(T) and NEAU-C8, isolated from rhizosphere soil and wheat root, respectively, collected from Langfang, Hebei Province, China. A polyphasic study was carried out to establish the taxonomic position of the two strains. Morphological and chemotaxonomic characteristics of the isolates coincided with the genus Glycomyces. Sequences analysis of the 16S rRNA gene also showed that the organisms belong to the genus Glycomyces and Glycomyces algeriensis is the highest sequence match for both strains...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
Mohammed Harir, Bellahcene Miloud, Maria Camilla Baratto, Simona Pollini, Gian Maria Rossolini, Lorenza Trabalzini, Enrico Fatarella, Rebecca Pogni
In the present study different actinomycete strains were collected and isolated from algerian Sahara soil with the aim to select novel enzymes with promising features for biotechnological applications. The Ms1 strain was selected, amongst the others, for its capability to produce melanin in different solid media. Ms1 chromosomal DNA was sequenced and the strain assigned to Streptomyces cyaneofuscatus sp. A tyrosinase (MW∼30kD) encoding sequence was identified and the corresponding enzyme was isolated and biochemically characterized...
November 10, 2017: Journal of Biotechnology
Qing Liu, Yu-Hua Xin, Yu-Guang Zhou, Wen-Xin Chen
Members of the bacterial genus Arthrobacter sensu lato are Gram-positive actinomycetes distributed worldwide and found in numerous environments including soil, water, glacier ice, and sewage. Homologous recombination is an important driving force in bacterial evolution, but its impact on Arthrobacter sensu lato evolution is poorly understood. We evaluated homologous recombination among 41 Arthrobacter sensu lato named species, using multilocus sequence analysis (MLSA). A high level of recombination was found, associated with strong diversification and a reticulate evolutionary pattern of Arthrobacter sensu lato...
November 3, 2017: Systematic and Applied Microbiology
Guoqing Niu
The prevalence of antimicrobial-resistant pathogens has highlighted the urgent need for new drugs. Actinomycetes have been the most prominent sources of natural products for drug discovery and development. Advances in genomics have inspired several emerging strategies to reinvigorate the field of natural product discovery, especially in actinomycete-derived natural products.
November 7, 2017: Trends in Biotechnology
Peng Ding, Juan-Luan Bai, Tian-Tian Wang, Yong Sun, Cheng-Liang Cao, Ji-Hong Jiang, Sheng Qin
An actinomycete strain, designated KLBMP S0039(T), was isolated from the rhizosphere soil of Lycium Linn., collected from the coastal region in Lianyungang, Jiangsu Province, eastern PR China, and was studied to determine its taxonomic position. The isolate showed a combination of morphological and chemotaxonomic properties typical of the members of the genus Nocardia. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were galactose, arabinose, glucose and ribose...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Zhi Qu, Xiao-Dong Bao, Qing-Yi Xie, You-Xing Zhao, Bing Yan, Hao-Fu Dai, Hui-Qin Chen
An actinomycete strain M4I47(T) was isolated from sediment from Megas Gialos, Syros, Greece. The results of phylogenetic analysis of the 16S rRNA gene sequence of M4I47(T) indicated that the highest similarity was with Actinoplanes atraurantiacus Y16(T) (98.9 %), Actinoplanes deccanensis IFO 13994(T) (98.8 %), Actinoplanes digitatis IFO 12512(T) (98.1 %) and Actinoplanes abujensis A4029(T) (98.0 %). The cell wall of the novel isolate contained meso-diaminopimelic acid and the whole-cell sugars were xylose, arabinose and glucose...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Song-Tao Zhang, Xiao-Ning Song, Ning Li, Ke Zhang, Guo-Shun Liu, Xue-Dong Li, Zhi-Zhong Wang, Xiao-Bing He, Guo-Feng Wang, Hui-Fang Shao
Soil microorganisms play a crucial role in cycling soil nutrients and providing organic nutrients for plant growth and development. Fertilisation balances soil fertility and quality, and affects soil microbial communities. Fertilisation is a frontier subject in agricultural and environmental sciences. Here we showed that the application of high-carbon basal fertiliser treatment could improve the tobacco yield and quality when compared to chemical fertiliser, high-carbon basal fertiliser and mixed high-carbon chemical fertiliser...
November 7, 2017: Research in Microbiology
Chongxi Liu, Chuanyu Han, Shanwen Jiang, Xueli Zhao, Yuanyuan Tian, Kai Yan, Xiangjing Wang, Wensheng Xiang
During a screening for novel and biotechnologically useful actinobacteria in insects, a novel actinobacteria with antifungal activity, designated strain 5H-CA11(T), was isolated from the head of an ant (Lasius flavus) and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence analysis showed that strain 5H-CA11(T) is a member of the genus Streptomyces, with the highest sequence similarity to Streptomyces scabrisporus DSM 41855(T) (98...
November 8, 2017: Current Microbiology
Patricia S Conville, Barbara A Brown-Elliott, Terry Smith, Adrian M Zelazny
Nocardia species are a complex group of organisms considered to belong to the aerobic actinomycetes. Of the validly described species, many have been implicated as the cause of serious human infections, especially in immunocompromised patients. The genus has a complicated taxonomic history; this is especially true for Nocardia asteroides, the type species of the genus and previously the most frequently reported nocardial taxon from human specimens. We provide background on the current taxonomy of Nocardia with a focus on clinically relevant species and discuss the currently available methods used to accurately identify isolates to the species, complex or group level...
November 8, 2017: Journal of Clinical Microbiology
Yern-Hyerk Shin, Suhyun Bae, Jaehoon Sim, Joonseong Hur, Shin-Il Jo, Jongheon Shin, Young-Ger Suh, Ki-Bong Oh, Dong-Chan Oh
Nicrophorusamides A and B (1 and 2) were discovered from a rare actinomycete, Microbacterium sp., which was isolated from the gut of the carrion beetle Nicrophorus concolor. The structures of the nicrophorusamides were established as new chlorinated cyclic hexapeptides bearing uncommon amino acid units mainly based on 1D and 2D NMR spectroscopic analysis. The absolute configurations of the amino acid residues 5-chloro-l-tryptophan, d-threo-β-hydroxyasparagine/d-asparagine, l-ornithine, l-allo-isoleucine, d-leucine, and d-valine were determined using Marfey's method and chemical derivatization with 2,3,4,6-tetra-O-acetyl-β-d-glucopyranosyl isothiocyanate followed by LC/MS analysis...
November 7, 2017: Journal of Natural Products
Shanwen Jiang, Chenyu Piao, Yang Yu, Peng Cao, Chenxu Li, Fan Yang, Mutong Li, Wensheng Xiang, Chongxi Liu
A novel actinomycete, designated strain 1H-SSA4(T), was isolated from the head of an ant (Camponotus japonicus Mayr) and was found to produce angucyclinone antibiotics. A polyphasic approach was used to determine the taxonomic status of strain 1H-SSA4(T). The DNA G+C content of the draft genome sequence, consisting of 11.4 Mbp, was 70.0 mol%. 16S rRNA gene sequence similarity studies showed that strain 1H-SSA4(T) belongs to the genus Streptomyces with the highest sequence similarity to Streptomyces hygroscopicus subsp...
November 7, 2017: International Journal of Systematic and Evolutionary Microbiology
Hao Wu, Bin Liu, Xiaoyun Ou, Shangli Pan, Yuanyuan Shao, Fuchang Huang
During an investigation exploring potential sources of novel thermophilic species and natural products, a novel thermophilic and alkaliphilic actinomycete with alkaline cellulase producing ability, designated strain 4-2-13(T), was isolated from soil of a tropical rainforest in Xishuangbanna, Yunnan province, China. The morphological and chemotaxonomic characteristics of strain 4-2-13(T) are consistent with those of the members of the genus Streptomyces. The strain forms extensively branched aerial mycelia and substrate mycelia...
November 7, 2017: Antonie Van Leeuwenhoek
Youyou Lu, Nana Wang, Jiao He, Yanfang Li, Xiaoning Gao, Lili Huang, Xia Yan
Saccharothrix yanglingensis Hhs.015, a new type of rare actinomycete, was isolated from the roots of cucumber. A novel chitinase gene was cloned from S. yanglingensis Hhs.015 and overexpressed as a soluble protein Chi6769 (77.9 kDa) in Escherichia coli. Chi6769 was purified by HisTrap HP affinity chromatography with optimal pH of 7.0. The enzymatic hydrolysis assay revealed that Chi6769 was capable of hydrolyzing chitin to (GlcNAc)3, (GlcNAc)2 and GlcNAc. (GlcNAc)2 was the main hydrolyzate. The antifungal activity result showed that Chi6769 exhibited strong antifungal activity toward Valsa mali 03-8...
November 1, 2017: Protein Expression and Purification
Nurul Syahirah Shamsol Anuar, Aeyshah Abang Kassim, Motoo Utsumi, Koji Iwamoto, Masafumi Goto, Kazuya Shimizu, Nor'azizi Othman, Zuriati Zakaria, Norio Sugiura, Hirofumi Hara
Geosmin and 2-methylisoborneol (MIB) outbreaks in tropical water bodies, such as Southeast Asia, by actinomycetes have not yet been elucidated in detail. Six Streptomyces isolates from lowland environments in Malaysia were selected and evaluated for their odor production under different temperatures. The gene responsible for the production of geosmin, geoA, was detected in all isolates, while only two isolates harbored tpc, which is responsible for 2-MIB production. This result suggested that geosmin and 2-MIB synthesis pathway genes already existed in the environment in the Tropics of Southeast Asia...
October 31, 2017: Microbes and Environments
Shi Ying Ma, Yong Sheng Xiao, Bo Zhang, Fen Li Shao, Zhi Kai Guo, Juan Juan Zhang, Rui Hua Jiao, Yang Sun, Qiang Xu, Ren Xiang Tan, Hui Ming Ge
Two novel enediyne-derived natural products, amycolamycins A and B (1 and 2), were characterized from a locust-associated actinomycete Amycolatopsis sp. HCa4. Amycolamycins A and B contain a unique 2-(cyclopenta[a]inden-5-yl)oxirane core with suspected enediyne polyketide biosynthetic origin. Sequencing and analysis of the acm biosynthetic gene cluster allowed us to propose the biosynthetic pathway of 1 and 2. Moreover, amycolamycin A (1) was selectively cytotoxic to the M231 breast cancer cell line.
November 1, 2017: Organic Letters
Eric D Brown, Sebastian S Gehrke, Garima Kumar, Nicole A Yokubynas, Jean-Philippe Côté, Wenliang Wang, Shawn French, Craig R MacNair, Gerard D Wright
Actinomycete secondary metabolites are a renowned source of antibacterial chemical scaffolds. Herein, we present a target-specific approach that increases the detection of antimetabolites from natural sources by screening actinomycete-derived extracts against nutrient transporter deletion strains. Based on the growth rescue patterns of a collection of 22 E. coli auxotrophic deletion strains representative of the major nutrient biosynthetic pathways, we demonstrate that antimetabolite detection from actinomycete-derived extracts prepared using traditional extraction platforms is masked by nutrient supplementation...
October 25, 2017: ACS Infectious Diseases
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