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Yan-Qin Yang, Hong-Xu Yin, Hai-Bo Yuan, Yong-Wen Jiang, Chun-Wang Dong, Yu-Liang Deng
In the present work, a novel infrared-assisted extraction coupled to headspace solid-phase microextraction (IRAE-HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) was developed for rapid determination of the volatile components in green tea. The extraction parameters such as fiber type, sample amount, infrared power, extraction time, and infrared lamp distance were optimized by orthogonal experimental design. Under optimum conditions, a total of 82 volatile compounds in 21 green tea samples from different geographical origins were identified...
2018: PloS One
Timothy R Entwistle, Adèle C Green, James E Fildes, Kyoko Miura
BACKGROUND: Heart and lung transplant recipients are at a substantially increased risk of cardiovascular disease (CVD). Since both low-fat and Mediterranean diets can reduce CVD in immunocompetent people at high risk, we assessed adherence among thoracic transplant recipients allocated to one or other of these diets for 12 months. METHODS: Forty-one transplant recipients (20 heart; 21 lung) randomized to a Mediterranean or a low-fat diet for 12 months received diet-specific education at baseline...
February 14, 2018: Nutrition Journal
Sanjeev Mariathasan, Shannon J Turley, Dorothee Nickles, Alessandra Castiglioni, Kobe Yuen, Yulei Wang, Edward E Kadel Iii, Hartmut Koeppen, Jillian L Astarita, Rafael Cubas, Suchit Jhunjhunwala, Romain Banchereau, Yagai Yang, Yinghui Guan, Cecile Chalouni, James Ziai, Yasin Şenbabaoğlu, Stephen Santoro, Daniel Sheinson, Jeffrey Hung, Jennifer M Giltnane, Andrew A Pierce, Kathryn Mesh, Steve Lianoglou, Johannes Riegler, Richard A D Carano, Pontus Eriksson, Mattias Höglund, Loan Somarriba, Daniel L Halligan, Michiel S van der Heijden, Yohann Loriot, Jonathan E Rosenberg, Lawrence Fong, Ira Mellman, Daniel S Chen, Marjorie Green, Christina Derleth, Gregg D Fine, Priti S Hegde, Richard Bourgon, Thomas Powles
Therapeutic antibodies that block the programmed death-1 (PD-1)-programmed death-ligand 1 (PD-L1) pathway can induce robust and durable responses in patients with various cancers, including metastatic urothelial cancer. However, these responses only occur in a subset of patients. Elucidating the determinants of response and resistance is key to improving outcomes and developing new treatment strategies. Here we examined tumours from a large cohort of patients with metastatic urothelial cancer who were treated with an anti-PD-L1 agent (atezolizumab) and identified major determinants of clinical outcome...
February 14, 2018: Nature
Arianna P Grove, Dionysios Liveris, Radha Iyer, Mary Petzke, Joseph Rudman, Melissa J Caimano, Justin D Radolf, Ira Schwartz
The alternative sigma factor RpoS plays a key role modulating gene expression in Borrelia burgdorferi, the Lyme disease spirochete, by transcribing mammalian host-phase genes and repressing σ(70)-dependent genes required within the arthropod vector. To identify cis regulatory elements involved in RpoS-dependent repression, we analyzed green fluorescent protein (GFP) transcriptional reporters containing portions of the upstream regions of the prototypical tick-phase genes ospAB, the glp operon, and bba74 As RpoS-mediated repression occurs only following mammalian host adaptation, strains containing the reporters were grown in dialysis membrane chambers (DMCs) implanted into the peritoneal cavities of rats...
August 22, 2017: MBio
Steven J Skates, Mark H Greene, Saundra S Buys, Phuong L Mai, Powel Brown, Marion Piedmonte, Gustavo Rodriguez, John O Schorge, Mark Sherman, Mary B Daly, Thomas Rutherford, Wendy R Brewster, David M O'Malley, Edward Partridge, John Boggess, Charles W Drescher, Claudine Isaacs, Andrew Berchuck, Susan Domchek, Susan A Davidson, Robert Edwards, Steven A Elg, Katie Wakeley, Kelly-Anne Phillips, Deborah Armstrong, Ira Horowitz, Carol J Fabian, Joan Walker, Patrick M Sluss, William Welch, Lori Minasian, Nora K Horick, Carol H Kasten, Susan Nayfield, David Alberts, Dianne M Finkelstein, Karen H Lu
Purpose: Women at familial/genetic ovarian cancer risk often undergo screening despite unproven efficacy. Research suggests each woman has her own CA125 baseline; significant increases above this level may identify cancers earlier than standard 6- to 12-monthly CA125 > 35 U/mL. Experimental Design: Data from prospective Cancer Genetics Network and Gynecologic Oncology Group trials, which screened 3,692 women (13,080 woman-screening years) with a strong breast/ovarian cancer family history or BRCA1/2 mutations, were combined to assess a novel screening strategy...
July 15, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Athanasios Dalakouras, Michèle Wassenegger, John N McMillan, Vinitha Cardoza, Ira Maegele, Elena Dadami, Miriam Runne, Gabi Krczal, Michael Wassenegger
In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotiana benthamiana plants to trigger RNA silencing. In contrast to mere siRNA applications, including spraying, syringe injection, and infiltration of siRNAs that all failed to induce RNA silencing, high pressure spraying of siRNAs resulted in efficient local and systemic silencing of the GFP transgene, with comparable efficiency as was achieved with biolistic siRNA introduction...
2016: Frontiers in Plant Science
Sean O'Mahony, James Gerhart, Ira Abrams, Michelle Greene, Rory McFadden, Sara Tamizuddin, Mitchell M Levy
AIM: Medical providers may face unique emotional challenges when confronted with the suffering of chronically ill, dying, and bereaved children. This study assessed the preliminary outcomes of participation in a group-based multimodal mindfulness training pilot designed to reduce symptoms of burnout and mental health symptoms in providers who interact with children in the context of end-of-life care. METHODS: A total of 13 medical providers who care for children facing life-threatening illness or bereaved children participated in a 9-session multimodal mindfulness session...
January 1, 2016: American Journal of Hospice & Palliative Care
Ashley J Dittmar, Allison A Drozda, Ira J Blader
The urgent need to develop new antimicrobial therapies has spawned the development of repurposing screens in which well-studied drugs and other types of compounds are tested for potential off-label uses. As a proof-of-principle screen to identify compounds effective against Toxoplasma gondii, we screened a collection of 1,120 compounds for the ability to significantly reduce Toxoplasma replication. A total of 94 compounds blocked parasite replication with 50% inhibitory concentrations of <5 µM. A significant number of these compounds are established inhibitors of dopamine or estrogen signaling...
March 2016: MSphere
S Zheng
Alternative splicing is a fundamental regulatory process of gene expression. Defects in alternative splicing can lead to various diseases, and modification of disease-causing splicing events presents great therapeutic promise. Splicing outcome is commonly affected by extracellular stimuli and signaling cascades that converge on RNA-binding splicing regulators. These trans-acting factors recognize cis-elements in pre-mRNA transcripts to affect spliceosome assembly and splice site choices. Identification of these splicing regulators and/or upstream modulators has been difficult and traditionally done by piecemeal...
2016: Methods in Enzymology
Isaac Adatto, Lauren Krug, Leonard Ira Zon
Light-dark cycles mimicking natural settings in a zebrafish facility are crucial for maintaining fish with an entrained circadian clock making them an ideal vertebrate model to study such rhythms. However, failure to provide optimal conditions to include complete darkness can lead to a disturbed circadian pacemaker affecting physiology and behavior in zebrafish. To meet building code requirements, the aquatics facility in use was outfitted with EXIT signs emitting a constant light. To determine if light radiating from the EXIT sign has an effect on zebrafish embryo production, 100 fish (1:1 m/f ratio) were split and housed at 10 fish/L...
June 2016: Zebrafish
Alexander Greene, Richard B Jones, Thomas W Wright, Ira M Parsons, Paul Saadi, Emilie V Cheung, Sandrine Polakovic, Matthew A Hamilton
Preoperative planning tools in shoulder arthroplasty are a recently developing technology with the advantage of being able to clearly assess patient anatomy and deformities before entering the OR. Addressing retroverted glenoids remains one of the most difficult aspects of primary shoulder arthroplasty. In this study, five surgeons were provided with a preoperative planning tool with posterior augmented glenoid implant options (0°, 8°, and 16°) to treat 10 cadaveric cases with a range of versions from 7...
December 2015: Bulletin of the Hospital for Joint Diseases
Anita A Kelkar, Javed Butler, Erik B Schelbert, Stephen J Greene, Arshed A Quyyumi, Robert O Bonow, Ira Cohen, Mihai Gheorghiade, Michael J Lipinski, Wei Sun, Dror Luger, Stephen E Epstein
Over the past 1.5 decades, numerous stem cell trials have been performed in patients with cardiovascular disease. Although encouraging outcome signals have been reported, these have been small, leading to uncertainty as to whether they will translate into significantly improved outcomes. A reassessment of the rationale for the use of stem cells in cardiovascular disease is therefore timely. Such a rationale should include analyses of why previous trials have not produced significant benefit and address whether mechanisms contributing to disease progression might benefit from known activities of stem cells...
November 3, 2015: Journal of the American College of Cardiology
Lei Zhang, Xumin Wang, Tao Liu, Guoliang Wang, Shan Chi, Cui Liu, Haiyang Wang
In this study, we fully sequenced the circular plastid genome of a brown alga, Undaria pinnatifida. The genome is 130,383 base pairs (bp) in size; it contains a large single-copy (LSC, 76,598 bp) and a small single-copy region (SSC, 42,977 bp), separated by two inverted repeats (IRa and IRb: 5,404 bp). The genome contains 139 protein-coding, 28 tRNA, and 6 rRNA genes; none of these genes contains introns. Organization and gene contents of the U. pinnatifida plastid genome were similar to those of Saccharina japonica...
2015: PloS One
Shuo Wang, Li-Zhi Gao
The complete chloroplast genome of green foxtail (Setaria viridis), a promising model system for C4 photosynthesis, is first reported in this study. The genome harbors a large single copy (LSC) region of 81 016 bp and a small single copy (SSC) region of 12 456  bp separated by a pair of inverted repeat (IRa and IRb) regions of 22 315 bp. GC content is 38.92%. The proportion of coding sequence is 57.97%, comprising of 111 (19 duplicated in IR regions) unique genes, 71 of which are protein-coding genes, four are rRNA genes, and 36 are tRNA genes...
September 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Claudia A Grillo, Gerardo G Piroli, Robert C Lawrence, Shayna A Wrighten, Adrienne J Green, Steven P Wilson, Randall R Sakai, Sandra J Kelly, Marlene A Wilson, David D Mott, Lawrence P Reagan
Insulin receptors (IRs) are expressed in discrete neuronal populations in the central nervous system, including the hippocampus. To elucidate the functional role of hippocampal IRs independent of metabolic function, we generated a model of hippocampal-specific insulin resistance using a lentiviral vector expressing an IR antisense sequence (LV-IRAS). LV-IRAS effectively downregulates IR expression in the rat hippocampus without affecting body weight, adiposity, or peripheral glucose homeostasis. Nevertheless, hippocampal neuroplasticity was impaired in LV-IRAS-treated rats...
November 2015: Diabetes
Inaya Ahmed, Kavita Dharmarajan, Amy Tiersten, Ira Bleiweiss, Hank Schmidt, Sheryl Green, Richard L Bakst
We are reporting a case of a 34-year-old woman with occult primary breast cancer discovered after initially presenting with neurological symptoms. She was successfully treated with neoadjuvant chemotherapy followed by definitive axillary lymph node dissection and ipsilateral whole breast radiotherapy. The case presented is unique due to the rarity of occult primary breast cancer, especially in light of her initial confounding neurological signs and symptoms, which highlights the importance of careful staging...
2015: Case Reports in Oncological Medicine
Denise A Yardley, Robert Weaver, Michelle E Melisko, Mansoor N Saleh, Francis P Arena, Andres Forero, Tessa Cigler, Alison Stopeck, Dennis Citrin, Ira Oliff, Rebecca Bechhold, Randa Loutfi, Agustin A Garcia, Scott Cruickshank, Elizabeth Crowley, Jennifer Green, Thomas Hawthorne, Michael J Yellin, Thomas A Davis, Linda T Vahdat
PURPOSE: Glycoprotein NMB (gpNMB), a negative prognostic marker, is overexpressed in multiple tumor types. Glembatumumab vedotin is a gpNMB-specific monoclonal antibody conjugated to the potent cytotoxin monomethyl auristatin E. This phase II study investigated the activity of glembatumumab vedotin in advanced breast cancer by gpNMB expression. PATIENTS AND METHODS: Patients (n = 124) with refractory breast cancer that expressed gpNMB in ≥ 5% of epithelial or stromal cells by central immunohistochemistry were stratified by gpNMB expression (tumor, low stromal intensity, high stromal intensity) and were randomly assigned 2:1 to glembatumumab vedotin (n = 83) or investigator's choice (IC) chemotherapy (n = 41)...
May 10, 2015: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Karoline B Kuchenbaecker, Susan J Ramus, Jonathan Tyrer, Andrew Lee, Howard C Shen, Jonathan Beesley, Kate Lawrenson, Lesley McGuffog, Sue Healey, Janet M Lee, Tassja J Spindler, Yvonne G Lin, Tanja Pejovic, Yukie Bean, Qiyuan Li, Simon Coetzee, Dennis Hazelett, Alexander Miron, Melissa Southey, Mary Beth Terry, David E Goldgar, Saundra S Buys, Ramunas Janavicius, Cecilia M Dorfling, Elizabeth J van Rensburg, Susan L Neuhausen, Yuan Chun Ding, Thomas V O Hansen, Lars Jønson, Anne-Marie Gerdes, Bent Ejlertsen, Daniel Barrowdale, Joe Dennis, Javier Benitez, Ana Osorio, Maria Jose Garcia, Ian Komenaka, Jeffrey N Weitzel, Pamela Ganschow, Paolo Peterlongo, Loris Bernard, Alessandra Viel, Bernardo Bonanni, Bernard Peissel, Siranoush Manoukian, Paolo Radice, Laura Papi, Laura Ottini, Florentia Fostira, Irene Konstantopoulou, Judy Garber, Debra Frost, Jo Perkins, Radka Platte, Steve Ellis, Andrew K Godwin, Rita Katharina Schmutzler, Alfons Meindl, Christoph Engel, Christian Sutter, Olga M Sinilnikova, Francesca Damiola, Sylvie Mazoyer, Dominique Stoppa-Lyonnet, Kathleen Claes, Kim De Leeneer, Judy Kirk, Gustavo C Rodriguez, Marion Piedmonte, David M O'Malley, Miguel de la Hoya, Trinidad Caldes, Kristiina Aittomäki, Heli Nevanlinna, J Margriet Collée, Matti A Rookus, Jan C Oosterwijk, Laima Tihomirova, Nadine Tung, Ute Hamann, Claudine Isaccs, Marc Tischkowitz, Evgeny N Imyanitov, Maria A Caligo, Ian G Campbell, Frans B L Hogervorst, Edith Olah, Orland Diez, Ignacio Blanco, Joan Brunet, Conxi Lazaro, Miquel Angel Pujana, Anna Jakubowska, Jacek Gronwald, Jan Lubinski, Grzegorz Sukiennicki, Rosa B Barkardottir, Marie Plante, Jacques Simard, Penny Soucy, Marco Montagna, Silvia Tognazzo, Manuel R Teixeira, Vernon S Pankratz, Xianshu Wang, Noralane Lindor, Csilla I Szabo, Noah Kauff, Joseph Vijai, Carol A Aghajanian, Georg Pfeiler, Andreas Berger, Christian F Singer, Muy-Kheng Tea, Catherine M Phelan, Mark H Greene, Phuong L Mai, Gad Rennert, Anna Marie Mulligan, Sandrine Tchatchou, Irene L Andrulis, Gord Glendon, Amanda Ewart Toland, Uffe Birk Jensen, Torben A Kruse, Mads Thomassen, Anders Bojesen, Jamal Zidan, Eitan Friedman, Yael Laitman, Maria Soller, Annelie Liljegren, Brita Arver, Zakaria Einbeigi, Marie Stenmark-Askmalm, Olufunmilayo I Olopade, Robert L Nussbaum, Timothy R Rebbeck, Katherine L Nathanson, Susan M Domchek, Karen H Lu, Beth Y Karlan, Christine Walsh, Jenny Lester, Alexander Hein, Arif B Ekici, Matthias W Beckmann, Peter A Fasching, Diether Lambrechts, Els Van Nieuwenhuysen, Ignace Vergote, Sandrina Lambrechts, Ed Dicks, Jennifer A Doherty, Kristine G Wicklund, Mary Anne Rossing, Anja Rudolph, Jenny Chang-Claude, Shan Wang-Gohrke, Ursula Eilber, Kirsten B Moysich, Kunle Odunsi, Lara Sucheston, Shashi Lele, Lynne R Wilkens, Marc T Goodman, Pamela J Thompson, Yurii B Shvetsov, Ingo B Runnebaum, Matthias Dürst, Peter Hillemanns, Thilo Dörk, Natalia Antonenkova, Natalia Bogdanova, Arto Leminen, Liisa M Pelttari, Ralf Butzow, Francesmary Modugno, Joseph L Kelley, Robert P Edwards, Roberta B Ness, Andreas du Bois, Florian Heitz, Ira Schwaab, Philipp Harter, Keitaro Matsuo, Satoyo Hosono, Sandra Orsulic, Allan Jensen, Susanne Kruger Kjaer, Estrid Hogdall, Hanis Nazihah Hasmad, Mat Adenan Noor Azmi, Soo-Hwang Teo, Yin-Ling Woo, Brooke L Fridley, Ellen L Goode, Julie M Cunningham, Robert A Vierkant, Fiona Bruinsma, Graham G Giles, Dong Liang, Michelle A T Hildebrandt, Xifeng Wu, Douglas A Levine, Maria Bisogna, Andrew Berchuck, Edwin S Iversen, Joellen M Schildkraut, Patrick Concannon, Rachel Palmieri Weber, Daniel W Cramer, Kathryn L Terry, Elizabeth M Poole, Shelley S Tworoger, Elisa V Bandera, Irene Orlow, Sara H Olson, Camilla Krakstad, Helga B Salvesen, Ingvild L Tangen, Line Bjorge, Anne M van Altena, Katja K H Aben, Lambertus A Kiemeney, Leon F A G Massuger, Melissa Kellar, Angela Brooks-Wilson, Linda E Kelemen, Linda S Cook, Nhu D Le, Cezary Cybulski, Hannah Yang, Jolanta Lissowska, Louise A Brinton, Nicolas Wentzensen, Claus Hogdall, Lene Lundvall, Lotte Nedergaard, Helen Baker, Honglin Song, Diana Eccles, Ian McNeish, James Paul, Karen Carty, Nadeem Siddiqui, Rosalind Glasspool, Alice S Whittemore, Joseph H Rothstein, Valerie McGuire, Weiva Sieh, Bu-Tian Ji, Wei Zheng, Xiao-Ou Shu, Yu-Tang Gao, Barry Rosen, Harvey A Risch, John R McLaughlin, Steven A Narod, Alvaro N Monteiro, Ann Chen, Hui-Yi Lin, Jenny Permuth-Wey, Thomas A Sellers, Ya-Yu Tsai, Zhihua Chen, Argyrios Ziogas, Hoda Anton-Culver, Aleksandra Gentry-Maharaj, Usha Menon, Patricia Harrington, Alice W Lee, Anna H Wu, Celeste L Pearce, Gerry Coetzee, Malcolm C Pike, Agnieszka Dansonka-Mieszkowska, Agnieszka Timorek, Iwona K Rzepecka, Jolanta Kupryjanczyk, Matt Freedman, Houtan Noushmehr, Douglas F Easton, Kenneth Offit, Fergus J Couch, Simon Gayther, Paul P Pharoah, Antonis C Antoniou, Georgia Chenevix-Trench
Genome-wide association studies (GWAS) have identified 12 epithelial ovarian cancer (EOC) susceptibility alleles. The pattern of association at these loci is consistent in BRCA1 and BRCA2 mutation carriers who are at high risk of EOC. After imputation to 1000 Genomes Project data, we assessed associations of 11 million genetic variants with EOC risk from 15,437 cases unselected for family history and 30,845 controls and from 15,252 BRCA1 mutation carriers and 8,211 BRCA2 mutation carriers (3,096 with ovarian cancer), and we combined the results in a meta-analysis...
February 2015: Nature Genetics
Edward N Libby, Pamela S Becker, Nicholas Burwick, Damian J Green, Leona Holmberg, William Ira Bensinger
Multiple myeloma is an incurable often devastating disease that is responsible for 1-2% of all cancers. Multiple myeloma is the second most common hematologic malignancy. Over the past two decades, advances in therapy have doubled life expectancy. Unfortunately, all patients ultimately relapse. Novel agents (immunomodulatory drugs and proteasome inhibitors) have changed the outlook for patients, but further breakthroughs are needed. Epigenetic treatments offer potential for advancing therapy by modifying oncogene responses...
February 2015: Expert Review of Hematology
Chris King, Esteban N Garza, Ronit Mazor, Jonathan L Linehan, Ira Pastan, Marion Pepper, David Baker
Immune responses can make protein therapeutics ineffective or even dangerous. We describe a general computational protein design method for reducing immunogenicity by eliminating known and predicted T-cell epitopes and maximizing the content of human peptide sequences without disrupting protein structure and function. We show that the method recapitulates previous experimental results on immunogenicity reduction, and we use it to disrupt T-cell epitopes in GFP and Pseudomonas exotoxin A without disrupting function...
June 10, 2014: Proceedings of the National Academy of Sciences of the United States of America
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