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https://www.readbyqxmd.com/read/28818649/sequence-analysis-of-mtdna-coi-barcode-region-revealed-three-haplotypes-within-culex-pipiens-assemblage
#1
Mona Koosha, Mohammad Ali Oshaghi, Mohammad Mehdi Sedaghat, Hassan Vatandoost, Shahyad Azari-Hamidian, Mohammad Reza Abai, Ahmad Ali Hanafi-Bojd, Fatemeh Mohtarami
Members of the Culex (Culex) pipiens assemblage are known vectors of deadly encephalitides, periodic filariasis, and West Nile virus throughout the world. However, members of this assemblage are morphologically indistinguishable or hard to distinguish and play distinct roles in transmission of the diseases. The current study aimed to provide further evidence on utility of the two most popular nuclear (ITS2-rDNA) and mitochondrial (COI barcode region) genetic markers to identify members of the assemblage. Culex pipiens assemblage specimens from different climate zones of Iran were collected and identified to species level based on morphological characteristics...
August 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28817640/evaluation-of-multilocus-marker-efficacy-for-delineating-mangrove-species-of-west-coast-india
#2
Ankush Ashok Saddhe, Rahul Arvind Jamdade, Kundan Kumar
The plant DNA barcoding is a complex and requires more than one marker(s) as compared to animal barcoding. Mangroves are diverse estuarine ecosystem prevalent in the tropical and subtropical zone, but anthropogenic activity turned them into the vulnerable ecosystem. There is a need to build a molecular reference library of mangrove plant species based on molecular barcode marker along with morphological characteristics. In this study, we tested the core plant barcode (rbcL and matK) and four promising complementary barcodes (ITS2, psbK-psbI, rpoC1 and atpF-atpH) in 14 mangroves species belonging to 5 families from West Coast India...
2017: PloS One
https://www.readbyqxmd.com/read/28811535/delimiting-species-of-protaphorura-collembola-onychiuridae-integrative-evidence-based-on-morphology-dna-sequences-and-geography
#3
Xin Sun, Feng Zhang, Yinhuan Ding, Thomas W Davies, Yu Li, Donghui Wu
Species delimitation remains a significant challenge when the diagnostic morphological characters are limited. Integrative taxonomy was applied to the genus Protaphorura (Collembola: Onychiuridae), which is one of most difficult soil animals to distinguish taxonomically. Three delimitation approaches (morphology, molecular markers and geography) were applied providing rigorous species validation criteria with an acceptably low error rate. Multiple molecular approaches, including distance- and evolutionary model-based methods, were used to determine species boundaries based on 144 standard barcode sequences...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28808384/molecular-authentication-of-the-traditional-medicinal-plant-lakshman-booti-smithia-conferta-sm-and-its-adulterants-through-dna-barcoding
#4
Suraj D Umdale, Parthraj R Kshirsagar, Manoj M Lekhak, Nikhil B Gaikwad
BACKGROUND: Smithia conferta Sm. is an annual herb widely used in Indian traditional medical practice and commonly known as "Lakshman booti" in Sanskrit. Morphological resemblance among the species of genus Smithia Aiton. leads to inaccurate identification and adulteration. This causes inconsistent therapeutic effects and also affects the quality of herbal medicine. AIM: This study aimed to generate potential barcode for authentication of S. conferta and its adulterants through DNA barcoding technique...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28793855/contrasting-morphology-with-molecular-data-an-approach-to-revision-of-species-complexes-based-on-the-example-of-european-phoxinus-cyprinidae
#5
Anja Palandačić, Alexander Naseka, David Ramler, Harald Ahnelt
BACKGROUND: Molecular taxonomy studies and barcoding projects can provide rapid means of detecting cryptic diversity. Nevertheless, the use of molecular data for species delimitation should be undertaken with caution. Especially the single-gene approaches are linked with certain pitfalls for taxonomical inference. In the present study, recent and historical species descriptions based upon morphology were used as primary species hypotheses, which were then evaluated with molecular data (including in type and historical museum material) to form secondary species hypotheses...
August 9, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28791201/comparative-mitogenomic-analysis-of-mirid-bugs-hemiptera-miridae-and-evaluation-of-potential-dna-barcoding-markers
#6
Juan Wang, Li Zhang, Qi-Lin Zhang, Min-Qiang Zhou, Xiao-Tong Wang, Xing-Zhuo Yang, Ming-Long Yuan
The family Miridae is one of the most species-rich families of insects. To better understand the diversity and evolution of mirids, we determined the mitogenome of Lygus pratenszs and re-sequenced the mitogenomes of four mirids (i.e., Apolygus lucorum, Adelphocoris suturalis, Ade. fasciaticollis and Ade. lineolatus). We performed a comparative analysis for 15 mitogenomic sequences representing 11 species of five genera within Miridae and evaluated the potential of these mitochondrial genes as molecular markers...
2017: PeerJ
https://www.readbyqxmd.com/read/28774799/discrimination-of-neotropical-anopheles-species-based-on-molecular-and-wing-geometric-morphometric-traits
#7
Giovan F Gómez, Margarita M Correa
Morphological similarities among closely related Anopheles species that differ in biological traits and malaria transmission represent a challenge in medical entomology; therefore, new tools are constantly tested to bring solutions. Particularly, in this work, a geometric morphometric analysis of wing geometry variation, based on morphologically and molecularly identified specimens, was applied for the discrimination of fourteen Anopheles species belonging to the Nyssorhynchus, Anopheles and Kerteszia subgenera...
August 1, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28771590/exploring-the-larval-fish-community-of-the-central-red-sea-with-an-integrated-morphological-and-molecular-approach
#8
Stamatina Isari, John K Pearman, Laura Casas, Craig T Michell, Joao Curdia, Michael L Berumen, Xabier Irigoien
An important aspect of population dynamics for coral reef fishes is the input of new individuals from the pelagic larval pool. However, the high biodiversity and the difficulty of identifying larvae of closely related species represent obstacles to more fully understanding these populations. In this study, we combined morphology and genetic barcoding (Cytochrome Oxidase I gene) to characterize the seasonal patterns of the larval fish community at two sites in close proximity to coral reefs in the central-north Red Sea: one shallower inshore location (50 m depth) and a nearby site located in deeper and more offshore waters (~ 500 m depth)...
2017: PloS One
https://www.readbyqxmd.com/read/28769719/the-subgenus-monotarsobius-in-the-iberian-peninsula-with-a-description-of-a-new-pseudo-cryptic-species-from-northern-spain-revealed-by-an-integrative-revision-of-lithobius-crassipes-l-koch-1862-chilopoda-lithobiomorpha-lithobiidae
#9
Karin Voigtländer, Etienne Iorio, Peter Decker, Jörg Spelda
The widespread European centipede species Lithobius (Monotarsobius) crassipes L. Koch, 1862 was revised using an integrative approach incorporating sequence data and morphology. The partial mitochondrial cytochrome c oxidase subunit I (COI) barcoding gene was amplified and sequenced for 21 individuals from northern Spain, France and Germany as well as for individuals of three other species of the subgenus Monotarsobius Verhoeff, 1905. The dataset was used for molecular phylogenetic analysis and genetic distance determination...
2017: ZooKeys
https://www.readbyqxmd.com/read/28769709/a-new-cryptic-species-of-nagiella-munroe-from-china-revealed-by-dna-barcodes-and-morphological-evidence-lepidoptera-crambidae-spilomelinae
#10
Misbah Ullah, Zhaofu Yang, Pingping Qiao, Yalin Zhang
Nagiella occultalis Misbah & Yang, sp. n. from China is described and illustrated. This new species is very similar to N. quadrimaculalis (Kollar, 1844) in general morphological characters of forewing and male genitalia. Molecular evidence shows that these two species diverge in COI barcode region by more than 3.2%. Sequence divergence among the two species is congruent with subtle morphological differences. Wing venation and male genitalia of the two species are compared and illustrated.
2017: ZooKeys
https://www.readbyqxmd.com/read/28769640/molecular-and-morphological-identification-of-biomphalaria-species-from-the-state-of-s%C3%A3-o-paulo-brazil
#11
Raquel Gardini Sanches Palasio, Marisa Cristina de Almeida Guimarães, Fernanda Pires Ohlweiler, Roseli Tuan
DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation...
2017: ZooKeys
https://www.readbyqxmd.com/read/28769617/a-survey-of-five-pireneitega-species-agelenidae-coelotinae-from-china
#12
Xiaoqing Zhang, Zhe Zhao, Guo Zheng, Shuqiang Li
Five species of Pireneitega spiders from China are surveyed, of which three are new to science: P. huashanensis Zhao & Li, sp. n. (♂♀), P. lushuiensis Zhao & Li, sp. n. (♂♀), P. xiyankouensis Zhao & Li, sp. n. (♂♀). Two known species are redescribed: P. liansui (Bao & Yin, 2004) and P. triglochinata (Zhu & Wang, 1991). The males of P. liansui and P. triglochinata (Zhu & Wang, 1991) are described for the first time. DNA barcodes for five species are documented for future use and as proof of molecular differences between species...
2017: ZooKeys
https://www.readbyqxmd.com/read/28765722/megafauna-of-the-uksrl-exploration-contract-area-and-eastern-clarion-clipperton-zone-in-the-pacific-ocean-echinodermata
#13
Diva J Amon, Amanda F Ziegler, Antonina Kremenetskaia, Christopher L Mah, Rich Mooi, Tim O'Hara, David L Pawson, Michel Roux, Craig R Smith
BACKGROUND: There is growing interest in mining polymetallic nodules from the abyssal Clarion-Clipperton Zone (CCZ) in the tropical Pacific Ocean. Despite being the focus of environmental studies for decades, the benthic megafauna of the CCZ remain poorly known. In order to predict and manage the environmental impacts of mining in the CCZ, baseline knowledge of the megafauna is essential. The ABYSSLINE Project has conducted benthic biological baseline surveys in the UK Seabed Resources Ltd polymetallic-nodule exploration contract area (UK-1)...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/28761780/dna-barcoding-of-forensically-important-blow-flies-diptera-calliphoridae-in-the-caribbean-region
#14
Sohath Z Yusseff-Vanegas, Ingi Agnarsson
Correct identification of forensically important insects, such as flies in the family Calliphoridae, is a crucial step for them to be used as evidence in legal investigations. Traditional identification based on morphology has been effective, but has some limitations when it comes to identifying immature stages of certain species. DNA-barcoding, using COI, has demonstrated potential for rapid and accurate identification of Calliphoridae, however, this gene does not reliably distinguish among some recently diverged species, raising questions about its use for delimitation of species of forensic importance...
2017: PeerJ
https://www.readbyqxmd.com/read/28761482/molecular-characterization-and-analysis-of-16s-ribosomal-dna-in-some-isolates-of-demodex-folicullorum
#15
Afrooz Daneshparvar, Gholamreza Mowlavi, Hamed Mirjalali, Homa Hajjaran, Iraj Mobedi, Saeed Reza Naddaf, Mohammadreza Shidfar, Mahsa Sadat Makki
BACKGROUND: Demodicosis is one of the most prevalent skin diseases resulting from infestation by Demodex mites. This parasite usually inhabits in follicular infundibulum or sebaceous duct and transmits through close contact with an infested host. METHODS: This study was carried from September 2014 to January 2016 at Tehran University of Medical Sciences, Tehran, Iran. DNA extraction and amplification of 16S ribosomal RNA was performed on four isolates, already obtained from four different patients and identified morphologically though clearing with 10% Potassium hydroxide (KOH) and microscopical examination...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28758818/genetic-characterization-of-bactrocera-fruit-flies-diptera-tephritidae-from-northeastern-india-based-on-dna-barcodes
#16
Arpana Manger, G T Behere, D M Firake, Bhagawati Sharma, N A Deshmukh, P D Firake, N S Azad Thakur, S V Ngachan
The Northeastern region of India, one of the mega biodiversity hot spots has enormous potential for the production of fruits and vegetables. Fruit flies of the genus Bactrocera Macquart are important pests of fruits and vegetables, and one of the limiting factors in successful production of these commodities. The relationship among some of the species is unclear due to their high molecular and morphological similarities. Moreover, due to the significant morphological resemblance between fruit fly species, reliable identification is very difficult task...
July 31, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28748632/first-molecular-identification-of-the-vertebrate-hosts-of-culicoides-imicola-in-europe-and-a-review-of-its-blood-feeding-patterns-worldwide-implications-for-the-transmission-of-bluetongue-disease-and-african-horse-sickness
#17
J Martínez-DE LA Puente, J Navarro, M Ferraguti, R Soriguer, J Figuerola
Culicoides (Diptera: Ceratopogonidae) are vectors of pathogens that affect wildlife, livestock and, occasionally, humans. Culicoides imicola (Kieffer, 1913) is considered to be the main vector of the pathogens that cause bluetongue disease (BT) and African horse sickness (AHS) in southern Europe. The study of blood-feeding patterns in Culicoides is an essential step towards understanding the epidemiology of these pathogens. Molecular tools that increase the accuracy and sensitivity of traditional methods have been developed to identify the hosts of potential insect vectors...
July 27, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28746445/bat-trypanosomes-from-tapaj%C3%A3-s-arapiuns-extractive-reserve-in-brazilian-amazon
#18
Bruno Bernal Szpeiter, Juliana Isabel Giuli da Silva Ferreira, Francisco Flávio Vieira de Assis, Felipe Nascimento Stelmachtchuk, Kleber da Cunha Peixoto, Daniel Ajzenberg, Antonio Humberto Hammad Minervino, Solange Maria Gennari, Arlei Marcili
Trypanosoma comprises flagellates able to infect several mammalian species and is transmitted by several groups of invertebrates. The order Chiroptera can be infected by the subgenera Herpetosoma, Schizotrypanum, Megatrypanum and Trypanozoon. In this study, we described the diversity of bat trypanosomes and inferred phylogenetic relationships among the trypanosomes from bats caught in Tapajós-Arapiuns Extractive Reserve (Resex) in Pará state. Trypanosomes from bats were isolated by means of hemoculture, and the molecular phylogeny was based on the trypanosome barcode (SSUrDNA V7V8 variable region)...
April 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28744301/three-dimensional-evaluation-on-ecotypic-diversity-of-traditional-chinese-medicine-a-case-study-of-artemisia-annua-l
#19
Lin Li, Brinckmann A Josef, Bing Liu, Sihao Zheng, Linfang Huang, Shilin Chen
Artemisinin is the first-line drug for anti-malaria recommended by the World Health Organization (WHO). As the sole natural plant source of artemisinin, ecotypes of Artemisia annua L. vary widely in artemisinin content between nations, and China is the main producing area of A. annua. Here we present a three-dimensional evaluation on ecotypic diversity of A. annua from 12 main producing areas in China using high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) method, DNA barcoding and ecological analyses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28743954/molecular-diversity-of-benthic-ctenophores-coeloplanidae
#20
Ada Alamaru, Bert W Hoeksema, Sancia E T van der Meij, Dorothée Huchon
Coeloplanidae, the largest family of benthic ctenophores, comprises 33 species, all described based on traditional morphological characteristics, such as coloration, length, and number of aboral papillae, which are highly variable and can be affected by fixation methods and environmental conditions. Thus, there is a need for reliable genetic markers to complement the morphological identifications at the species level. Here, we analyzed 95 specimens from 11 morphologically distinct species of benthic ctenophores from the Red Sea and Sulu Sea, and tested selected regions of four genetic markers (ITS1, 18S rRNA, 28S rRNA and COI) for their ability to differentiate between species...
July 25, 2017: Scientific Reports
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