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https://www.readbyqxmd.com/read/29456730/the-regulation-and-function-of-microrna-377-rassf8-signaling-axis-in-gastric-cancer
#1
Xiaobo Bo, Yusheng Chen, Weizhong Sheng, Yuda Gong, Haiyu Wang, Weidong Gao, Bo Zhang
Gastric cancer is a major cause of cancer-associated mortality worldwide. The aberrant expression of microRNA (miRNA) is involved in tumorigenesis. Ras proteins transfer information from the extracellular environment to internal cellular compartments and are essential in numerous signal transduction pathways. To investigate the regulation, function and clinical significance of the miRNA377/Ras association domain family (RASSF) 8 signaling axis in gastric cancer, reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, cell counting kit-8, western blotting, and Transwell assays were used...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29456052/performance-of-a-highly-successful-outbreak-strain-of-mycobacterium-tuberculosis-in-a-multifaceted-approach-to-bacterial-fitness-assessment
#2
Noemí Yokobori, Beatriz López, Johana Monteserin, Roxana Paul, Andrea Von Groll, Anandi Martin, Brenda Marquina-Castillo, Juan Carlos Palomino, Rogelio Hernández-Pando, María Del Carmen Sasiain, Viviana Ritacco
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain...
January 31, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29454898/dietary-values-of-forsythia-suspensa-extract-in-penaeus-monodon-under-normal-rearing-and-vibrio-parahaemolyticus-3hp-vp-3hp-challenge-conditions-effect-on-growth-intestinal-barrier-function-immune-response-and-immune-related-gene-expression
#3
Jia-Jun Xie, Xu Chen, Tian-Yu Guo, Shi-Wei Xie, Hao-Hang Fang, Zhen-Lu Liu, Yan-Mei Zhang, Li-Xia Tian, Yong-Jian Liu, Jin Niu
Two trials were conducted to determine the effects of dietary Forsythia suspensa extract (FSE) on shrimp, Penaeus monodon, first on growth performance, second on the immune response and immune related gene expression of shrimp. In trial 1, shrimp (mean initial wet weight about 3.02 g) were fed with five diets containing 0% (basal diet), 0.01%, 0.02%, 0.04% and 0.06% FSE in triplicate for 60 days. Growth performance (final body wet weight, FBW; weight gain, WG; biomass gain, BG) of shrimp fed FSE diets were higher (P < 0...
February 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29450711/renal-fat-fraction-and-diffusion-tensor-imaging-in-patients-with-early-stage-diabetic-nephropathy
#4
Yuan-Cheng Wang, Yinglian Feng, Chun-Qiang Lu, Shenghong Ju
OBJECTIVE: To investigate the renal fat fraction and water molecular diffusion features in patients with early-stage DN using Dixon imaging and diffusion tensor imaging (DTI). METHODS: Sixty-one type 2 diabetics (normoalbuminuria: n = 40; microalbuminuria: n = 21) and 34 non-diabetic volunteers were included. All participants received three-point Dixon imaging and DTI using a 3.0-T magnetic resonance imager. The fat fraction [FF] and DTI features [fractional anisotropy (FA), apparent diffusion coefficient (ADC), tract counts and length from DTI tractography] were collected...
February 15, 2018: European Radiology
https://www.readbyqxmd.com/read/29447904/intraspecific-venom-variation-in-southern-african-scorpion-species-of-the-genera-parabuthus-uroplectes-and-opistophthalmus-scorpiones-buthidae-scorpionidae
#5
Stephan Schaffrath, Lorenzo Prendini, Reinhard Predel
Scorpion venoms comprise cocktails of proteins, peptides, and other molecules used for immobilizing prey and deterring predators. The composition and efficacy of scorpion venoms appears to be taxon-specific due to a coevolutionary arms race with prey and predators that adapt at the molecular level. The taxon-specific components of scorpion venoms can be used as barcodes for species identification if the amount of intraspecific variation is low and the analytical method is fast, inexpensive and reliable. The present study assessed the extent of intraspecific variation in newly regenerated venom collected in the field from geographically separated populations of four southern African scorpion species: three buthids, Parabuthus granulatus (Ehrenberg, 1831), Uroplectes otjimbinguensis (Karsch, 1879), and Uroplectes planimanus (Karsch, 1879), and one scorpionid, Opistophthalmus carinatus (Peters, 1861)...
February 12, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29446543/cyclin-dependent-kinase-inhibitor-2a-b-gene-deletions-are-markers-of-poor-prognosis-in-indian-children-with-acute-lymphoblastic-leukemia
#6
Manisha Agarwal, Sameer Bakhshi, Sadanand N Dwivedi, Madhulika Kabra, Rashmi Shukla, Rachna Seth
BACKGROUND: Cyclin dependent kinase inhibitor 2A/B (CDKN2A/B) genes are implicated in many malignancies including acute lymphoblastic leukemia (ALL). These tumor suppressor genes, with a key regulatory role in cell cycle are located on chromosome 9p21.3. Previous studies involving CDKN2A/B gene deletions have shown mixed associations with survival outcome in childhood ALL. PROCEDURE: Hundred and four newly diagnosed children with ALL (1-14 years) were enrolled in this study...
February 15, 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29435709/the-prevalence-and-isolated-subtypes-of-bk-polyomavirus-reactivation-among-patients-infected-with-human-immunodeficiency-virus-1-in-southeastern-china
#7
Caiqin Hu, Ying Huang, Juwei Su, Mengyan Wang, Qihui Zhou, Biao Zhu
BK polyomavirus (BKPyV) is an opportunistic infectious pathogen that is associated with hemorrhagic cystitis and nephropathy, mainly in transplant recipients and human immunodeficiency virus 1 (HIV-1) infected patients. However, molecular characterization studies of BKPyV in China are rare. This study was designed to elucidate the prevalence and to determine the main subtypes of BKPyV among HIV-1-infected patients in southeastern China. In addition, the increased incidences for BKPyV reactivation were analyzed...
February 12, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29435117/replication-of-hepatitis-e-virus-in-the-ovary-and-promotion-of-oocyte-apoptosis-in-rabbits-infected-with-hev-4
#8
Junqing An, Tianlong Liu, Ruiping She, Qiaoxing Wu, Jijing Tian, Ruihan Shi, Wenzhuo Hao, Xinxin Ren, Yue Yang, Yiyao Lu, Yifei Yang, Yuanheng Wu
Hepatitis E virus (HEV) infection can induce infertility and miscarriage in pregnant women and infect neonates through vertical transmission. However, the mechanism of infertility and vertical transmission remains unclear. In the present study, we evaluated the replication of HEV in the ovary and structural and molecular changes induced by HEV after intraperitoneal injection of HEV in rabbits. Positive- and negative-strand HEV RNA was detected in the ovaries at 28 and 49 days post-infection. Positive HEV open reading frames 2 and 3 signals were observed in the ovaries by immunohistochemistry staining...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29435021/long-lasting-memory-of-cellular-immunity-in-a-chronic-myeloid-leukemia-patient-maintains-molecular-response-5-after-cessation-of-dasatinib
#9
Tatsuro Jo, Kazuhiro Noguchi, Shizuka Hayashi, Sadaharu Irie, Risa Hayase, Haruna Shioya, Youhei Kaneko, Kensuke Horio, Jun Taguchi
Tyrosine kinase inhibitors (TKIs), including imatinib, dasatinib and nilotinib are primarily used in the initial treatment of chronic phase (CP)-chronic myeloid leukemia (CML), as CMLs harbor the BCR-ABL fusion product. An increased number of lymphocytes and large granular lymphocytes (LGLs) have been observed in patients treated with dasatinib, but not other TKIs. The LGLs have been reported to be primarily natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). In the present study, a CP-CML patient who has maintained molecular response 5 for >2...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434961/effect-and-molecular-mechanism-of-mtor-inhibitor-rapamycin-on-temozolomide-induced-autophagic-death-of-u251-glioma-cells
#10
Bing Li, Chun Zhou, Liang Yi, Lunshan Xu, Minhui Xu
Glioma is a malignant tumor of the glial tissue that is difficult to excise through surgery, with poor patient prognosis. The use of chemotherapeutic drugs alone to treat glioma following surgery results in a high probability of sequelae, such as tumor recurrence. The present study investigated the effects of a novel treatment combination on glioma cells and determined the molecular mechanisms underlying its action. The effect of temozolomide (TMZ) combined with rapamycin (RAPA) on the TMZ-induced autophagic death of U251 glioma cells was examined...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434947/association-between-inflammation-and-cancer-stem-cell-phenotype-in-breast-cancer
#11
Young Ju Jeong, Hoon Kyu Oh, Sung Hwan Park, Jin Gu Bong
Inflammation and cancer stem cells (CSCs) are becoming increasingly recognized as components of tumorigenesis in breast cancer. In the present study, the association between inflammation and BCSC phenotype was evaluated in human breast cancer tissue. Immunohistochemical staining for cluster of differentiation (CD)24, 44, 4, 8 and 68 was performed using tissue microarray blocks containing 47 consecutive cases of invasive breast carcinoma and 10 normal breast tissue samples. The levels of inflammatory modulators and cytokines, and intratumoral or peritumoral lymphocyte infiltration, were assessed...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434850/modulation-of-g2-m-cell-cycle-arrest-and-apoptosis-by-luteolin-in-human-colon-cancer-cells-and-xenografts
#12
Zhicheng Chen, Bo Zhang, Feng Gao, Renjie Shi
The aim of the present study was to investigate the underlying molecular mechanisms of the potent cell cycle inhibition and apoptotic effect of luteolin on LoVo human colon cancer cells. In the present study, Cell Counting kit-8 assay revealed that luteolin exerted notable cytotoxicity on LoVo cells in a dose- and time-dependent manner, with a 50% inhibitory concentration value of 66.70 and 30.47 µmol/l at the time points of 24 and 72 h, respectively. Flow cytometric analysis confirmed that luteolin promoted cell cycle arrest at the G2/M phase, and subsequently induced cell apoptosis...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29429403/comparison-of-the-performance-of-laboratory-tests-in-the-diagnosis-of-feline-infectious-peritonitis
#13
Angelica Stranieri, Alessia Giordano, Saverio Paltrinieri, Chiara Giudice, Valentina Cannito, Stefania Lauzi
We compared the performance of clinicopathologic and molecular tests used in the antemortem diagnosis of feline infectious peritonitis (FIP). From 16 FIP and 14 non-FIP cats, we evaluated retrospectively the sensitivity, specificity, and likelihood ratios (LRs) of serum protein electrophoresis, α 1 -acid glycoprotein (AGP) on peripheral blood, screening reverse-transcription nested PCR (RT-nPCR) on the 3'-untranslated region (3'-UTR), and spike (S) gene sequencing on peripheral blood, body cavity effusions, and tissue, as well as body cavity cytology and delta total nucleated cell count (ΔTNC)...
February 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29429260/-the-influence-and-mechanisms-of-purple-sweet-potato-anthocyanins-on-the-growth-of-bladder-cancer-biu87-cell
#14
W L Li, G H Ji, X Z Zhang, H Y Yu
Objective: To observe the effect of purple sweet potato anthocyanins on the proliferation of bladder cancer cell line BIU87 and to investigate the molecular mechanisms. Methods: Bladder cancer BIU87 cells were cultured and exposed to anthocyanins at the different concentrations of 100, 200, 400, and 800 μg/ml respectively. The growth inhibition of anthocyanins on BIU87 cells were evaluated by morphometry and cell counting kit-8 (CCK-8) assay, and the cell apoptosis rate was detected by Flow cytometry (FCM)...
February 6, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29428682/counting-of-peripheral-extracellular-vesicles-in-multiple-sclerosis-patients-by-an-improved-nanoplasmonic-assay-and-dynamic-light-scattering
#15
Antonia Mallardi, Nicoletta Nuzziello, Maria Liguori, Carlo Avolio, Gerardo Palazzo
Extracellular vesicles (EVs) are vesicles naturally secreted by the majority of human cells. Being composed by a closed phospholipid bilayer secluding proteins and RNAs they are used to transfer molecular information to other cells, thereby influencing the recipient cell functions. Despite the increasingly recognized relevance of EVs, the clarification of their physiological role is hampered by the lack of suitable analytical tools for their quantification and characterization. In this study, we have implemented a nanoplasmonic assay, previously proposed for the purity of the EV fractions, to achieve a robust analytical protocol in order to quantify the total phospholipid concentration (CPL) and the EVs number...
February 5, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29426482/comparison-of-early-detection-of-fasciola-hepatica-in-experimentally-infected-merino-sheep-by-real-time-pcr-coproantigen-elisa-and-sedimentation
#16
Nichola Eliza Davies Calvani, Sarah Deanna George, Peter Andrew Windsor, Russell David Bush, Jan Šlapeta
Fasciolosis due to infection with Fasciola hepatica, Fasciola gigantica or their hybrids is a significant global cause of livestock production loss. Infection is commonly diagnosed by a labour-intensive sedimentation and faecal egg count (FEC), which has limited throughput and is only applicable after completion of the 8-12 week pre-patent period (PPP). A commercially-available ELISA for the detection of coprological antigen (coproELISA) enables detection prior to the completion of the PPP and is suitable for diagnosis of larger sample sizes, although the sensitivity reported under experimental infection settings can be difficult to replicate in the field, particularly in cattle...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29420338/curcumin-reverses-irinotecan-resistance-in-colon-cancer-cell-by-regulation-of-epithelial-mesenchymal-transition
#17
Chunhong Zhang, Yangjie Xu, Haowen Wang, Gang Li, Han Yan, Zhenghua Fei, Yunsheng Xu, Wenfeng Li
The objective of this study was to investigate the effect and the mechanism by which curcumin reverses irinotecan-induced chemotherapy resistance in colon cancer. Construction of irinotecan-resistant colon cancer model LoVo/CPT-11R cells was performed by increasing drug concentration. The Cell Counting Kit-8 assay was used to detect inhibition of proliferation; cell morphology was observed by an optical microscope. Quantitative RT-PCR and western blotting were performed to detect molecular marker expressions during epithelial-mesenchymal transition (EMT); drug-resistant cells were treated with curcumin at different concentrations and Cell Counting Kit-8 was reperformed to detect cell proliferation after treatments...
February 7, 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29413515/baseline-serum-cxcl10-and-il-12-levels-may-predict-severe-asthmatics-responsiveness-to-omalizumab
#18
Maho Suzukawa, Hisako Matsumoto, Nobuharu Ohshima, Hiroyuki Tashimo, Isao Asari, Tomoko Tajiri, Akio Niimi, Hiroyuki Nagase, Hirotoshi Matsui, Nobuyuki Kobayashi, Shunsuke Shoji, Ken Ohta
BACKGROUND: Omalizumab, a humanized anti-IgE monoclonal antibody, is the first molecularly targeted drug for severe asthmatics. However, responses to omalizumab vary widely among patients. OBJECTIVES: This study aimed to assess the potential of baseline serum cytokine levels as predictors of responsiveness to omalizumab. METHODS: Thirty-one patients with severe, persistent asthma were enrolled in this study and administered omalizumab for at least 1 year...
January 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29413382/molecular-detection-of-harmful-cyanobacteria-and-expression-of-their-toxin-genes-in-dutch-lakes-using-multi-probe-rna-chips
#19
Dedmer B Van de Waal, Delphine Guillebault, Amparo Alfonso, Inés Rodríguez, Luis M Botana, Ronald Bijkerk, Linda K Medlin
Harmful cyanobacterial blooms are a major threat to water quality and human health. Adequate risk assessment is thus required, which relies strongly on comprehensive monitoring. Here, we tested novel multi-probe RNA chips developed in the European project, μAqua, to determine the abundance of harmful cyanobacterial species and expression of selected toxin genes in six Dutch lakes. All of the targeted cyanobacterial genera, except for Planktothrix, were detected using the microarray, with predominance of Dolichospermum and Microcystis signals, of which the former was found across all sites and detected by the probes for Anabaena where it was formerly placed...
February 2018: Harmful Algae
https://www.readbyqxmd.com/read/29410647/emerging-understanding-of-the-mechanism-of-action-for-dimethyl-fumarate-in-the-treatment-of-multiple-sclerosis
#20
REVIEW
Elizabeth A Mills, Magdalena A Ogrodnik, Andrew Plave, Yang Mao-Draayer
Dimethyl fumarate (DMF) is an effective treatment option for relapsing-remitting multiple sclerosis (MS), but its therapeutic mechanism of action has not been fully elucidated. A better understanding of its mechanism will allow for the development of assays to monitor its clinical efficacy and safety in patients, as well as guide the development of the next generation of therapies for MS. In order to build the foundation for determining its mechanism, we reviewed the manner in which DMF alters lymphocyte subsets in MS patients, its impact on clinical efficacy and safety, as well as its molecular effects in cellular and animal models...
2018: Frontiers in Neurology
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