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https://www.readbyqxmd.com/read/29244483/biomembrane-permeabilization-statistics-of-individual-leakage-events-harmonize-the-interpretation-of-vesicle-leakage
#1
Stefan Braun, Šárka Pokorná, Radek Šachl, Martin Hof, Heiko Heerklotz, Maria Hoernke
The mode of action of membrane-active molecules such as antimicrobial, anticancer, cell penetrating, and fusion peptides and their synthetic mimics, transfection agents and drug permeation enhancers, biological signaling molecules (e.g., quorum sensing), involves either the general or local destabilization of the target membrane or the formation of defined, rather stable pores. Some effects aim at killing the cell, others need to be limited in space and time to avoid serious damage. Biological tests reveal translocation of compounds and cell death but do not provide a detailed, mechanistic, and quantitative understanding of the modes of action and their molecular basis...
December 15, 2017: ACS Nano
https://www.readbyqxmd.com/read/29243345/deoxythymidine-pterin-fluorescent-adduct-formation-by-a-photosensitized-proccess
#2
Sandra Estébanez, Andrés H Thomas, Carolina Lorente
A new fluorescent compound was isolated from UV-A irradiated aqueous solutions containing pterin (Ptr) and 2´deoxythymidine (dT) in anaerobic conditions. Pterins are widespread in the living systems in small amounts, but they are accumulated in some pathological situations. Under UV-A radiation, pterins are photochemically active, fluorescent and photosensitize the generation of singlet oxygen (1O2(1Δg)). The isolated compound was structurally characterized, by liquid chromatography coupled to tandem mass spectrometry, and its photophysical properties were studied by time correlated single photon counting technique...
December 15, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29242472/photophore-counts-in-the-deep-sea-commercial-shrimp-i-aristeus-i-i-alcocki-i-ramadan-1938-crustacea-decapoda-aristeidae-with-a-revised-key-to-the-indo-west-pacific-species-of-the-genus
#3
Tin-Yam Chan, Appukuttannair Biju Kumar, Chien-Hui Yang
The availability of abundant fresh material of Aristeus alcocki Ramadan, 1938 from India allowed the evaluation of the variation in the numbers of photophores on the pereiopods in this species, as well as other diagnostic characters for species discrimination. Although the pereiopodal photophore counts in A. alcocki largely overlap with those of A. semidentatus Bate, 1888, it is found that A. alcocki is unique in the Indo-West Pacific species of the genus by the lower end of the cervical carina considerably farther away from the branchiostegal carina...
October 6, 2017: Zootaxa
https://www.readbyqxmd.com/read/29239038/gastroenteropancreatic-neuroendocrine-neoplasms-selected-pathology-review-and-molecular-updates
#4
REVIEW
Siaw M Chai, Ian S Brown, M Priyanthi Kumarasinghe
Gastroenteropancreatic (GEP) neuroendocrine neoplasms can be broadly separated into well- and poorly differentiated categories. Tumours within each category have similarities in morphology and immunophenotype, but vary in grade, behaviour, molecular signature and responses to therapy. The aetiology of these differences is multifactorial. Site of origin, mucosal milieu and hereditary influences are some of the currently known factors. Given these differences, staging and grading systems continue to evolve, and the most recent World Health Organization classification of pancreatic neuroendocrine neoplasms reflects this by introducing a grade 3 neuroendocrine tumour category for morphologically well-differentiated tumours with an elevated Ki-67 proliferation index and/or mitotic count...
January 2018: Histopathology
https://www.readbyqxmd.com/read/29238567/mind-the-gap-integrating-taxonomic-approaches-to-assess-ant-diversity-at-the-southern-extreme-of-the-atlantic-forest
#5
Priscila Elena Hanisch, Pablo D Lavinia, Andrew V Suarez, Darío Alejandro Lijtmaer, Maurice Leponce, Carolina Ivon Paris, Pablo Luis Tubaro
Understanding patterns of species diversity relies on accurate taxonomy which can only be achieved by long-term natural history research and the use of complementary information to establish species boundaries among cryptic taxa. We used DNA barcoding to characterize the ant diversity of Iguazú National Park (INP), a protected area of the Upper Paraná Atlantic Forest ecoregion, located at the southernmost extent of this forest. We assessed ant diversity using both cytochrome c oxidase subunit 1 (COI) sequences and traditional morphological approaches, and compared the results of these two methods...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29237430/antitumor-effect-of-quercetin-on-y79-retinoblastoma-cells-via-activation-of-jnk-and-p38-mapk-pathways
#6
Haojie Liu, Ming Zhou
BACKGROUND: Quercetin (QCT) is a flavonol present in many vegetables, it is proved to show chemo preventive effect against lung, cervical, prostate, breast and colon cancer due to its anti-inflammatory, anti-tumor and anti-oxidant property. Looking into the reported chemo-preventive effect we speculated antitumor activity in retinoblastoma (RB) Y79 cells, we also studied the molecular mechanism for antitumor activity. METHODS: The effect of QCT on Y79 cell viability count was done by cell counting kit, cell cycle distribution, apoptosis studies and mitochondrial membrane potential was evaluated by flow cytometry...
December 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29236133/pesticide-analysis-by-pulsed-flow-modulation-gcxgc-ms-with-cold-ei-an-alternative-to-gc-ms-ms
#7
Uri Keshet, Paulina Goldshlag, Aviv Amirav
We explored the use of pulsed flow modulation (PFM) two-dimensional comprehensive gas chromatography (GCxGC) mass spectrometry with supersonic molecular beams (SMB) (also named Cold electron ionization (EI)) for achieving universal pesticide analysis in agricultural products. The use of GCxGC serves as an alternative to MS-MS in the needed reduction of matrix interference while enabling full-scan MS operation for universal pesticide analysis with reduced number of false negatives. Matrix interference is further reduced with Cold EI in view of the enhancement of the molecular ions...
December 13, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29235850/a-digital-protocol-for-chemical-analysis-at-ultra-low-concentrations-by-surface-enhanced-raman-scattering
#8
Carlos Diego L de Albuquerque, Regivaldo Gomes Sobral Filho, Ronei Jesus Poppi, Alexandre G Brolo
Single molecule surface-enhanced Raman Spectroscopy (SM-SERS) has the potential to revolutionize quantitative analysis at ultra-low concentrations (less than 1 nM). However, there are no established protocols to generalize the application of this technique in analytical chemistry. Here, a protocol for quantification at ultra-low concentrations using SM-SERS is proposed. The approach aims to take advantage of the stochastic nature of the single-molecule regime to achieved lower limits of detection (LOD) and quantification (LOQ)...
December 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29230021/markers-of-microglia-in-post-mortem-brain-samples-from-patients-with-alzheimer-s-disease-a-systematic-review
#9
REVIEW
K E Hopperton, D Mohammad, M O Trépanier, V Giuliano, R P Bazinet
Neuroinflammation is proposed as one of the mechanisms by which Alzheimer's disease pathology, including amyloid-β plaques, leads to neuronal death and dysfunction. Increases in the expression of markers of microglia, the main neuroinmmune cell, are widely reported in brains from patients with Alzheimer's disease, but the literature has not yet been systematically reviewed to determine whether this is a consistent pathological feature. A systematic search was conducted in Medline, Embase and PsychINFO for articles published up to 23 February 2017...
December 12, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/29228450/mir-21-enhanced-glioma-cells-resistance-to-carmustine-via-decreasing-spry2-expression
#10
G-B Wang, J-H Liu, J Hu, K Xue
OBJECTIVE: Gliomas are accompanied with high mortality owning to their invasive peculiarity and vulnerability to drug resistance. miR-21 is a vital oncogenic miRNA that regulates drug resistance of tumor cells. This study aims to elucidate the function of miR-21 in human glioma cells resistant to carmustine (BCNU) and to demonstrate the underlying molecular mechanism. MATERIALS AND METHODS: BCNU-sensitive cells (SWOZ2 cells) were transfected with miR-21 agomir and negative control, and BCNU-resistance cells (SWOZ2-BCNU cells) were transfected with miR-21 antagomir and negative control...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228449/igfbp2-induces-spry1-expression-via-nf-%C3%AE%C2%BAb-signaling-pathway-in-glioblastoma-multiforme-gbm
#11
Z-S Yuan, Y Cao, Z-Y Li
OBJECTIVE: Gliomas are accompanied with high mortality owning to their invasive peculiarity and vulnerability to drug resistance. miR-21 is a vital oncogenic miRNA that regulates drug resistance of tumor cells. This study aims to elucidate the function of miR-21 in human glioma cells resistant to carmustine (BCNU) and to demonstrate the underlying molecular mechanism. MATERIALS AND METHODS: BCNU-sensitive cells (SWOZ2 cells) were transfected with miR-21 agomir and negative control, and BCNU-resistance cells (SWOZ2-BCNU cells) were transfected with miR-21 antagomir and negative control...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228425/up-regulation-of-linc00346-inhibits-proliferation-of-non-small-cell-lung-cancer-cells-through-mediating-jak-stat3-signaling-pathway
#12
F Wang, J-G Chen, L-L Wang, Z-Z Yan, S-P Chen, X-G Wang
OBJECTIVE: To investigate the expression levels of LINCRNA00346 in tissues and cells in patients with non-small cell lung cancer (NSCLC) and its biological function. PATIENTS AND METHODS: The relative expression levels of LINC 00346 in 70 cases of tissues and cells in NSCLC patients were detected via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The specific interference sequences of LINC 00346 were designed and the transfection efficiency after 48 h was detected...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29228419/expression-and-clinical-significance-of-long-non-coding-rna-ghet1-in-pancreatic-cancer
#13
H-Y Zhou, H Zhu, X-Y Wu, X-D Chen, Z-G Qiao, X Ling, X-M Yao, J-H Tang
OBJECTIVE: To investigate the expression level and biological function of long non-coding RNA gastric carcinoma high expressed transcript 1 (lncRNA-GHET1) in pancreatic ductal adenocarcinoma (pancreatic cancer for short), to analyze the correlation between the expression of GHET1 and clinicopathological features and to explore the role and clinical significance of GHET1 in the development and progression of pancreatic cancer. PATIENTS AND METHODS: The relative expression of GHET1 in 5 human pancreatic cancer cell lines was detected by quantitative Real-time polymerase chain reaction (qRT-PCR)...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29226682/first-evaluation-of-radioiodinated-flavonoids-as-necrosis-avid-agents-and-application-in-the-early-assessment-of-tumor-necrosis
#14
Jiajia Liang, Ziping Sun, Dongjian Zhang, Qiaomei Jin, Lingqiao Cai, Lin Ma, Wei Liu, Yicheng Ni, Jian Zhang, Zhiqi Yin
A rapid and accurate identification of necrotic tissues is of great importance to define disease severity, predict prognosis and monitor responses to therapies. To seek necrosis-avid agents with clinically translational potential, we first evaluated the necrosis avidity of flavonoids in rodent models of muscular, myocardial and tumoral necrosis. In this study, the necrosis avidity of eight radioiodinated 5,7-dihydroxyflavones was tested by ex vivo gamma counting, histochemical staining, and autoradiography in mouse models of ethanol-induced muscular necrosis...
December 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29226135/a-novel-cyclophilin-b-gene-in-the-red-tide-dinoflagellate-cochlodinium-polykrikoides-molecular-characterizations-and-transcriptional-responses-to-environmental-stresses
#15
Sofia Abassi, Hui Wang, Bum Soo Park, Jong-Woo Park, Jang-Seu Ki
The marine dinoflagellate Cochlodinium polykrikoides is one of the most common ichthyotoxic species that causes harmful algal blooms (HABs), which leads to ecological damage and huge economic loss in aquaculture industries. Cyclophilins (CYPs) belong to the immunophilin superfamily, and they may play a role in the survival mechanisms of the dinoflagellate in stress environments. In the present study, we identified a novel cyclophilin gene from C. polykrikoides and examined physiological and gene transcriptional responses to biocides copper sulphate (CuSO4) and sodium hypochlorite (NaOCl)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29224316/-effects-of-npm1-gene-expression-on-acute-myeloid-leukemia-cell-lines-and-its-mechanism
#16
S W Qiu, Y L Wan, M Wang, J X Wang
Objective: To investigate the impact and mechanism of NPM1 gene expression on acute myeloid leukemia (AML) cell lines. Methods: Human AML cell line U937 and HL-60 cells were transfected with NPM1 plasmid to establish stable clones, and the high NPM1 protein expression (NPM1(hi)) clones were screened by Western blot. The cell proliferation was assayed by methylthiazolyl tetrazolium bromide (MTT) , cell cycle and cell apoptosis by flow cytometric, cell colony formation by microscope count, the molecular pathways related to cell cycle by Western blot...
November 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29222293/current-treatment-algorithm-for-the-management-of-lower-risk-mds
#17
REVIEW
Aristoteles Giagounidis
Lower risk myelodysplastic syndromes (MDS), defined as MDS with a Revised International Prognostic Scoring System score ≤3.5 points, will remain a challenging entity in 2018. Supportive care continues to be the linchpin of treatment, although the options to reduce transfusion needs are broadening. To achieve red blood cell transfusion independence in non-del(5q) patients, erythropoiesis-stimulating agents remain a mainstay of therapy as long as endogenous erythropoietin levels are <500 U/L (and preferably <200 U/L)...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29221447/disruption-of-postnatal-folliculogenesis-and-development-of-ovarian-tumor-in-a-mouse-model-with-aberrant-transforming-growth-factor-beta-signaling
#18
Yang Gao, Xin Fang, David F Vincent, David W Threadgill, Laurent Bartholin, Qinglei Li
BACKGROUND: Transforming growth factor beta (TGFB) superfamily signaling is implicated in the development of sex cord-stromal tumors, a category of poorly defined gonadal tumors. The aim of this study was to determine potential effects of dysregulated TGFB signaling in the ovary using Cre recombinase driven by growth differentiation factor 9 (Gdf9) promoter known to be expressed in oocytes. METHODS: A mouse model containing constitutively active TGFBR1 (TGFBR1CA) using Gdf9-iCre (termed TGFBR1-CAG9Cre) was generated...
December 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29221207/chst6-mutation-screening-and-endoplasmatic-reticulum-stress-in-macular-corneal-dystrophy
#19
Liyuan Wang, Xianling Tang, Xiaolin Lv, Encheng Sun, Donglai Wu, Changlin Wang, Ping Liu
Macular corneal dystrophy (MCD) is an autosomal recessive disorder mainly caused by gene mutations of carbohydrate sulfotransferase (CHST6) leading to bilateral visual impairment. Because the mechanism underlying this degeneration remains poorly understood, we investigated molecular alterations and pathways that may be involved in MCD in this issue. Different mutation sites were screened by direct sequencing of the coding region of CHST6. In addition, we described morphological changes in MCD keratocytes by light microscopy and electron microscopy and determined the relationship between the development of this disease and the occurrence of apoptosis through flow cytometry, cell counting kit-8, colony formation assay and other experiments...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29218526/glut4-translocation-in-single-muscle-cells-in-culture-epitope-detection-by-immunofluorescence
#20
Javier R Jaldin-Fincati, Philip J Bilan, Amira Klip
GLUT4 is the major glucose transporter in skeletal muscle. GLUT4 cycles to and from the plasma membrane and its exocytic rate is accelerated by insulin and muscle contraction to achieve a new steady state with more GLUT4 proteins at the muscle cell surface. To gain a better understanding of the molecular and cellular mechanisms that govern GLUT4 protein recycling, we developed an in vitro model in which myc-epitope-tagged GLUT4 or GLUT4-GFP is expressed in L6 skeletal muscle cells. The myc-epitope is inserted into an exofacial domain that is accessible to anti-myc antibodies from the outside of non-permeabilized cells, allowing one to count the number of transporters at the cell surface...
2018: Methods in Molecular Biology
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