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https://www.readbyqxmd.com/read/15759119/in-vitro-mutation-and-selection-of-doubled-haploid-brassica-napus-lines-with-improved-resistance-to-sclerotinia-sclerotiorum
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S Liu, H Wang, J Zhang, B D L Fitt, Z Xu, N Evans, Y Liu, W Yang, X Guo
This paper describes a new protocol to develop doubled-haploid (DH) Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. In this protocol, haploid seedlings derived from microspore cultures of B. napus were used to produce haploid calli for in vitro mutation-selection. For routine screening, mutation was induced by EMS (ethylmethane sulfonate) or occurred spontaneously, and screening for resistant mutants occurred on media with added oxalic acid (OA) as a selection agent. In tests with selected lines, the optimal concentration of EMS for mutation was determined to be 0...
June 2005: Plant Cell Reports
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