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JOURNAL ARTICLE
Jalal Taneera, Abdul Khader Mohammed, Anila Khalique, Bashair M Mussa, Nabil Sulaiman, Yasser Bustanji, Mohamed A Saleh, Mohamed Madkour, Eman Abu-Gharbieh, Waseem El-Huneidi
BACKGROUND AND AIMS: The protein phosphatase 1 regulatory inhibitor subunit 1A (PPP1R1A) has been linked with insulin secretion and diabetes mellitus. Yet, its full significance in pancreatic β-cell function remains unclear. This study aims to elucidate the role of the PPP1R1A gene in β-cell biology using human pancreatic islets and rat INS-1 (832/13) cells. RESULTS: Disruption of Ppp1r1a in INS-1 cells was associated with reduced insulin secretion and impaired glucose uptake; however, cell viability, ROS, apoptosis or proliferation were intact...
April 3, 2024: Life Sciences
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JOURNAL ARTICLE
Haile Berihulay Gebreselase, Hailemichael Nigussie, Changfa Wang, Chenglong Luo
Goats belong to a group of animals called small ruminants and are critical sources of livelihood for rural people. Genomic sequencing can provide information ranging from basic knowledge about goat diversity and evolutionary processes that shape genomes to functional information about genes/genomic regions. In this study, we exploited a whole-genome sequencing data set to analyze the genetic diversity, population structure and selection signatures of 44 individuals belonging to 5 Ethiopian goat populations: 12 Aberegalle (AB), 5 Afar (AF), 11 Begait (BG), 12 Central highlands (CH) and 5 Meafure (MR) goats...
January 18, 2024: Animals: An Open Access Journal From MDPI
#3
JOURNAL ARTICLE
Paul Richard Grimm, Anamaria Tatomir, Lena L Rosenbaek, Bo Young Kim, Dimin Li, Eric J Delpire, Robert A Fenton, Paul A Welling
Consumption of low dietary potassium, common with ultra-processed foods, activates the thiazide-sensitive sodium chloride cotransporter (NCC) via the WNK-SPAK kinase pathway to induce salt retention and elevate blood pressure (BP). However, it remains unclear how high potassium "DASH-like" diets inactivate the cotransporter and whether this decreases BP. A transcriptomic screen identified Ppp1C⍺, encoding PP1A, as a potassium up-regulated gene, and its negative regulator, Ppp1r1a, as a potassium-suppressed gene in the kidney...
September 7, 2023: Journal of Clinical Investigation
#4
JOURNAL ARTICLE
Magnus I Magnusson, Bjarni A Agnarsson, Jon G Jonasson, Thordur Tryggvason, Famke Aeffner, Louise le Roux, Droplaug N Magnusdottir, Helga S Gunnarsdottir, Kristín K Alexíusdóttir, Kristbjorg Gunnarsdottir, Emilia Söebech, Hjaltey Runarsdottir, Erna M Jonsdottir, Bjarney S Kristinsdottir, Sigurgeir Olafsson, Hildur Knutsdottir, Unnur Thorsteinsdottir, Magnus O Ulfarsson, Daniel F Gudbjartsson, Jona Saemundsdottir, Olafur T Magnusson, Gudmundur L Norddahl, J E Vivienne Watson, Thorunn Rafnar, Sigrun H Lund, Kari Stefansson
BACKGROUND: The TNM system is used to assess prognosis after colorectal cancer (CRC) diagnosis. Other prognostic factors reported include histopathological assessments of the tumour, tumour mutations and proteins in the blood. As some of these factors are strongly correlated, it is important to evaluate the independent effects they may have on survival. METHODS: Tumour samples from 2162 CRC patients were visually assessed for amount of tumour stroma, severity of lymphocytic infiltrate at the tumour margins and the presence of lymphoid follicles...
August 18, 2023: British Journal of Cancer
#5
JOURNAL ARTICLE
Renfei Zhu, Cheng Gao, Qiuqi Feng, Haitao Guan, Jianjun Wu, Hrishikesh Samant, Fan Yang, Xuehao Wang
BACKGROUND: Ferroptosis is a form of iron-dependent cell death with increased free iron and massive lipid peroxidation. The discovery of ferroptosis offers insights into hepatocellular carcinoma (HCC) treatment. However, post-transcriptional regulation mechanisms of ferroptosis in HCC remain to be elucidated. The present study explored ferroptosis-related genes and their post-transcriptional regulation mechanisms in HCC. METHODS: A ferroptosis score was computed in The Cancer Genome Atlas (TCGA) cohort via gene set variation analysis (GSVA), and ferroptosis-related genes were screened by differential expression and correlation analyses...
December 2022: Annals of Translational Medicine
#6
JOURNAL ARTICLE
Xixi Wu, Yin Wang, Mi Yang, Yingqiao Wang, Xiaoqing Wang, Longshan Zhang, Liwei Liao, Nan Li, Mengyuan Mao, Jian Guan, Feng Ye
Novel and accurate biomarkers are needed for early detection and progression evaluation of hepatocellular carcinoma (HCC). Protein phosphatase 1 regulatory subunit 1A (PPP1R1A) has been studied in cancer biology; however, the expression pattern and biological function of PPP1R1A in HCC are unclear. The differentially expressed genes (DEGs) in HCC were screened by The Cancer Genome Atlas (TCGA) database. Real-time PCR and immunohistochemistry (IHC) assay were used to detect the expression of PPP1R1A in BALB/c mice, human normal tissues and corresponding tumor tissues, especially HCC...
August 26, 2022: Human Cell
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JOURNAL ARTICLE
Amarinder Singh Thind, Bruce Ashford, Dario Strbenac, Jenny Mitchell, Jenny Lee, Simon A Mueller, Elahe Minaei, Jay R Perry, Sydney Ch'ng, N Gopalakrishna Iyer, Jonathan R Clark, Ruta Gupta, Marie Ranson
Metastatic cutaneous squamous cell carcinoma (CSCC) is a highly morbid disease requiring radical surgery and adjuvant therapy, which is associated with a poor prognosis. Yet, compared to other advanced malignancies, relatively little is known of the genomic landscape of metastatic CSCC. We have previously reported the mutational signatures and mutational patterns of CCCTC-binding factor (CTCF) regions in metastatic CSCC. However, many other genomic components (indel signatures, non-coding drivers, and structural variants) of metastatic CSCC have not been reported...
2022: Frontiers in Oncology
#8
JOURNAL ARTICLE
Michal Tamáš, Stanislava Pankratova, Peter Schjerling, Casper Soendenbroe, Ching-Yan Chloé Yeung, Cristian Pablo Pennisi, Jens R Jakobsen, Michael R Krogsgaard, Michael Kjaer, Abigail L Mackey
Insight into the bidirectional signaling between primary human myogenic cells and neurons is lacking. For this purpose, human myogenic cells were derived from the semitendinosus and gracilis muscles of five healthy individuals and co-cultured with cerebellar granule neurons from two litters of 7-day-old Wistar rat pups, in muscle medium or neural medium, alongside monocultures of myogenic cells or neurons. RT-PCR was performed to determine human mRNA levels of GAPDH, Ki67, myogenin, and MUSK, and the acetylcholine receptor subtypes CHRNA1, CHRNB1, CHRNG, CHRND, and CHRNE, and rat mRNA levels of GAPDH, Fth1, Rack1, vimentin, Cdh13, and Ppp1r1a...
November 2021: Physiological Reports
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JOURNAL ARTICLE
Junli Wang, Qi Zhang, Fukang Shi, Dipesh Kumar Yadav, Zhengtao Hong, Jianing Wang, Tingbo Liang, Xueli Bai
Purpose: Hepatocellular carcinoma (HCC) is one of the most prevalent malignant diseases worldwide and has a poor prognosis. Gene-based prognostic models have been reported to predict the overall survival of patients with HCC. Unfortunately, most of the genes used in earlier prognostic models lack prospective validation and, thus, cannot be used in clinical practice. Methods: Candidate genes were selected from GEPIA (Gene Expression Profiling Interactive Analysis), and their associations with patients' survival were confirmed by RT-PCR using cDNA tissue microarrays established from patients with HCC after radical resection...
2021: Frontiers in Genetics
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JOURNAL ARTICLE
Deepti Anand, Salma Al Saai, Sanjaya K Shrestha, Carrie E Barnum, Shinichiro Chuma, Salil A Lachke
Mutations/deficiency of TDRD7 , encoding a tudor domain protein involved in post-transcriptional gene expression control, causes early onset cataract in humans. While Tdrd7 is implicated in the control of key lens mRNAs, the impact of Tdrd7 deficiency on microRNAs (miRNAs) and how this contributes to transcriptome misexpression and to cataracts, is undefined. We address this critical knowledge-gap by investigating Tdrd7 -targeted knockout ( Tdrd7-/- ) mice that exhibit fully penetrant juvenile cataracts. We performed Affymetrix miRNA 3...
2021: Frontiers in Cell and Developmental Biology
#11
JOURNAL ARTICLE
Luis Rodrigo Cataldo, Neelanjan Vishnu, Tania Singh, Ludivine Bertonnier-Brouty, Sara Bsharat, Cheng Luan, Erik Renström, Rashmi B Prasad, Malin Fex, Hindrik Mulder, Isabella Artner
The amplification of glucose-stimulated insulin secretion (GSIS) through incretin signaling is critical for maintaining physiological glucose levels. Incretins, like glucagon-like peptide 1 (GLP1), are a target of type 2 diabetes drugs aiming to enhance insulin secretion. Here we show that the protein phosphatase 1 inhibitor protein 1A (PPP1R1A), is expressed in β-cells and that its expression is reduced in dysfunctional β-cells lacking MafA and upon acute MafA knock down. MafA is a central regulator of GSIS and β-cell function...
May 2021: Metabolism: Clinical and Experimental
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JOURNAL ARTICLE
Wei Liao, Yanchen Liu, Lixiang Wang, Xiao Cai, Hong Xie, Faping Yi, Rongzhong Huang, Chui Fang, Peng Xie, Jian Zhou
Chronic stress is a significant risk factor for depression as well as anxiety disorders. Yet, the stress-induced specific and common molecular dysregulations of these disorders have not been fully understood. Previously, we constructed a chronic mild stress (CMS) rat model to separate and obtain depression-susceptible, anxiety-susceptible, and insusceptible groups. In this study, the prefrontal cortical proteomes of the three stressed groups were comparatively profiled utilizing isobaric tags for relative and absolute quantitation (iTRAQ)-coupled tandem mass spectrometry approach...
February 24, 2021: Translational Psychiatry
#13
JOURNAL ARTICLE
Ling Chen, Zijin Xiang, Xueru Chen, Xiuting Zhu, Xiangdong Peng
BACKGROUND: Kidney renal clear cell carcinoma (KIRC) is a potentially fatal urogenital disease. It is a major cause of renal cell carcinoma and is often associated with late diagnosis and poor treatment outcomes. More evidence is emerging that genetic models can be used to predict the prognosis of KIRC. This study aimed to develop a model for predicting the overall survival of KIRC patients. RESULTS: We identified 333 differentially expressed genes (DEGs) between KIRC and normal tissues from the Gene Expression Omnibus (GEO) database...
September 3, 2020: Hereditas
#14
JOURNAL ARTICLE
Wen Luo, Changxin Xu, Sarah Phillips, Aliza Gardenswartz, Jeremy M Rosenblum, Janet Ayello, Stephen L Lessnick, Huai-Xiang Hao, Mitchell S Cairo
Ewing sarcoma (ES) is a malignant pediatric bone and soft tissue tumor. Patients with metastatic ES have a dismal outcome which has not been improved in decades. The major challenge in the treatment of metastatic ES is the lack of specific targets and rational combinatorial therapy. We recently found that protein phosphatase 1 regulatory subunit 1A (PPP1R1A) is specifically highly expressed in ES and promotes tumor growth and metastasis in ES. In the current investigation, we show that PPP1R1A regulates ES cell cycle progression in G1/S phase by down-regulating cell cycle inhibitors p21Cip1 and p27Kip1 , which leads to retinoblastoma (Rb) protein hyperphosphorylation...
May 12, 2020: Oncotarget
#15
JOURNAL ARTICLE
Sheng-Hong Shi, Jing Jiang, Wei Zhang, Long Sun, Xu-Jun Li, Chao Li, Qi-Dong Ge, Zhi-Gang Zhuang
Purpose: The function of long noncoding RNAs (lncRNA) in breast cancer metastasis remains largely unknown. In this work, the role of HOXC-AS3 in breast cancer progression was investigated. Methods: By using Cancer Genome Atlas (TCGA) Database, we investigated the expression of HOXC-AS3 in breast cancer and explored the association between HOXC-AS3 expression and prognosis. Then, we studied the biological function of HOXC-AS3 in cell migration and invasion both in vitro and in vivo . Furthermore, the target miRNA of HOXC-AS3, and the target mRNA of miR-3922-5p were proved...
December 4, 2019: Cancer Investigation
#16
JOURNAL ARTICLE
Michele Solimena, Anke M Schulte, Lorella Marselli, Florian Ehehalt, Daniela Richter, Manuela Kleeberg, Hassan Mziaut, Klaus-Peter Knoch, Julia Parnis, Marco Bugliani, Afshan Siddiq, Anne Jörns, Frédéric Burdet, Robin Liechti, Mara Suleiman, Daniel Margerie, Farooq Syed, Marius Distler, Robert Grützmann, Enrico Petretto, Aida Moreno-Moral, Carolin Wegbrod, Anke Sönmez, Katja Pfriem, Anne Friedrich, Jörn Meinel, Claes B Wollheim, Gustavo B Baretton, Raphael Scharfmann, Everson Nogoceke, Ezio Bonifacio, Dorothée Sturm, Birgit Meyer-Puttlitz, Ugo Boggi, Hans-Detlev Saeger, Franco Filipponi, Mathias Lesche, Paolo Meda, Andreas Dahl, Leonore Wigger, Ioannis Xenarios, Mario Falchi, Bernard Thorens, Jürgen Weitz, Krister Bokvist, Sigurd Lenzen, Guy A Rutter, Philippe Froguel, Manon von Bülow, Mark Ibberson, Piero Marchetti
AIMS/HYPOTHESIS: Pancreatic islet beta cell failure causes type 2 diabetes in humans. To identify transcriptomic changes in type 2 diabetic islets, the Innovative Medicines Initiative for Diabetes: Improving beta-cell function and identification of diagnostic biomarkers for treatment monitoring in Diabetes (IMIDIA) consortium ( www.imidia.org ) established a comprehensive, unique multicentre biobank of human islets and pancreas tissues from organ donors and metabolically phenotyped pancreatectomised patients (PPP)...
March 2018: Diabetologia
#17
JOURNAL ARTICLE
W Luo, C Xu, J Ayello, F Dela Cruz, J M Rosenblum, S L Lessnick, M S Cairo
Protein phosphatase inhibitors are often considered as tumor promoters. Protein phosphatase 1 regulatory subunit 1A (PPP1R1A) is a potent protein phosphatase 1 (PP1) inhibitor; however, its role in tumor development is largely undefined. Here we characterize, for the first time, the functions of PPP1R1A in Ewing sarcoma (ES) pathogenesis. We found that PPP1R1A is one of the top ranked target genes of EWS/FLI, the master regulator of ES, and is upregulated by EWS/FLI via a GGAA microsatellite enhancer element...
February 8, 2018: Oncogene
#18
JOURNAL ARTICLE
Yuya Sugano, Chiara Cianciolo Cosentino, Dominique Loffing-Cueni, Stephan C F Neuhauss, Johannes Loffing
Understanding the molecular basis of the complex regulatory networks controlling renal ion transports is of major physiological and clinical importance. In this study, we aimed to identify evolutionarily conserved critical players in the function of the renal distal convoluted tubule (DCT) by a comparative transcriptomic approach. We generated a transgenic zebrafish line with expression of the red fluorescent mCherry protein under the control of the zebrafish DCT-specific promoter of the thiazide-sensitive NaCl cotransporter (NCC)...
August 2017: Pflügers Archiv: European Journal of Physiology
#19
JOURNAL ARTICLE
Huaqiang Zhou, Qinchang Chen, Wulin Tan, Zeting Qiu, Si Li, Yiyan Song, Shaowei Gao
Pancreatic neuroendocrine tumors are relatively rare pancreatic neoplasms over the world. Investigations about molecular biology of PNETs are insufficient for nowadays. We aimed to explore the expression of messenger RNA and regulatory processes underlying pancreatic neuroendocrine tumors from different views. The expression profile of GSE73338 were downloaded, including samples with pancreatic neuroendocrine tumors. First, the Limma package was utilized to distinguish the differentially expressed messenger RNA...
August 20, 2017: Gene
#20
JOURNAL ARTICLE
Nobuhiro Wada, Satoru Yamanaka, Junko Shibato, Randeep Rakwal, Satoshi Hirako, Yuzuru Iizuka, Hyounju Kim, Akiyo Matsumoto, Ai Kimura, Fumiko Takenoya, Genta Yasunaga, Seiji Shioda
This study investigates effects of dipeptide balenine, as a major component of whale meat extract (hereafter, WME), supplementation on senescence-accelerated mouse prone 8 (SAMP8), an Alzheimer's disease (AD) model at level of learning and memory formation and brain expression profiles genome-wide in brain. Mice fed experimental balenine (+ WME) supplemented diet for 26 weeks were subjected to four behavioral tests - open field, Y-maze, new object recognition, and water-filled multiple T-maze - to examine effects on learning and memory...
December 2016: Genomics Data
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