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https://www.readbyqxmd.com/read/27813404/high-throughput-discovery-of-aptamers-for-sandwich-assays
#1
Andrew T Csordas, Anna Jørgensen, Jinpeng Wang, Emily Gruber, Qiang Gong, Elizabeth R Bagley, Margaret A Nakamoto, Michael Eisenstein, H Tom Soh
Sandwich assays are among the most powerful tools in molecular detection. These assays use "pairs" of affinity reagents so that the detection signal is generated only when both reagents bind simultaneously to different sites on the target molecule, enabling highly sensitive and specific measurements in complex samples. Thus, the capability to efficiently screen affinity reagent pairs at a high throughput is critical. In this work, we describe an experimental strategy for screening "aptamer pairs" at a throughput of 10(6) aptamer pairs per hour-which is many orders of magnitude higher than the current state of the art...
November 4, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27781272/gingival-crevicular-fluid-tissue-blood-vessel-type-plasminogen-activator-and-plasminogen-activator-inhibitor-2-levels-in-patients-with-rheumatoid-arthritis-effects-of-nonsurgical-periodontal-therapy
#2
Ş Kurgan, C Önder, N Balcı, Ö Fentoğlu, F Eser, M Balseven, M A Serdar, D N Tatakis, M Günhan
BACKGROUND AND OBJECTIVE: The aim of this study was to evaluate the effect of nonsurgical periodontal therapy on clinical parameters and gingival crevicular fluid levels of tissue/blood vessel-type plasminogen activator (t-PA) and plasminogen activator inhibitor-2 (PAI-2) in patients with periodontitis, with or without rheumatoid arthritis (RA). MATERIAL AND METHODS: Fifteen patients with RA and chronic periodontitis (RA-P), 15 systemically healthy patients with chronic periodontitis (H-P) and 15 periodontally and systemically healthy volunteers (C) were included in the study...
October 26, 2016: Journal of Periodontal Research
https://www.readbyqxmd.com/read/27694320/polyphosphate-co-localizes-with-factor-xii-on-platelet-bound-fibrin-and-augments-its-plasminogen-activator-activity
#3
Joanne L Mitchell, Ausra S Lionikiene, Georgi Georgiev, Anja Klemmer, Chelsea Brain, Paul Y Kim, Nicola J Mutch
Activated factor XII (FXIIa) has plasminogen activator capacity but its relative contribution to fibrinolysis is considered marginal compared to urokinase and tissue plasminogen activator. Polyphosphate (polyP) is released from activated platelets and mediates FXII activation. Here we investigate the contribution of polyP to the plasminogen activator function of αFXIIa. We show that both polyP70, of the chain length found in platelets (60-100-mer) and platelet-derived polyP, significantly augment the plasminogen activation capacity of αFXIIa...
September 30, 2016: Blood
https://www.readbyqxmd.com/read/27660159/a-clinical-study-on-single-visit-root-canal-retreatments-on-consecutive-173-patients-frequency-of-periapical-complications-and-clinical-success-rate
#4
Tan Firat Eyuboglu, Kezban Olcay, Mutlu Özcan
OBJECTIVES: This study assessed the outcome measures of single-visit root canal retreatments and frequency of periapical complications considering preoperative, intraoperative and postoperative factors. MATERIALS AND METHODS: Between November 2011 and December 2012, in 173 patients, a total of 234 endodontically treated teeth were retreated in a single appointment by one experienced endodontist. Five teeth were extracted and 119 teeth were lost to follow-up yielding to 110 teeth (47 %) to be examined by two calibrated examiners for the outcome of healing (periapical index score-PAI ≤2; no signs or symptoms) or non-healing (presence of apical periodontitis-PAI >2; signs or symptoms)...
September 22, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27644043/circulating-fibronectin-and-plasminogen-activator-inhibitor-2-levels-as-possible-predictors-of-recurrent-placental-syndrome-an-exploratory-study
#5
Carmen A H Severens-Rijvers, Salwan Al-Nasiry, Chahinda Ghossein-Doha, Sara Marzano, Hugo Ten Cate, Bjorn Winkens, Marc A E Spaanderman, Louis L H Peeters
BACKGROUND/AIM: Placental syndromes (PS) are characterized by endothelial dysfunction complicating placental dysfunction. Possible markers for endothelial dysfunction and amount of trophoblast are fibronectin and plasminogen activator inhibitor-2 (PAI-2), respectively. We aimed (1) to determine whether in women with recurrent PS (rPS), this complication is preceded by deviating fibronectin- and PAI-2-levels, and (2) whether this is dependent on pre-pregnant plasma volume (PV). METHODS: In 36 former patients, we determined fibronectin- and PAI-2-levels in blood-samples collected preconceptionally and at 12-16 weeks in their next pregnancy...
September 20, 2016: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/27600972/leucocyte-expression-of-genes-implicated-in-the-plasminogen-activation-cascade-is-modulated-by-yoghurt-peptides
#6
Georgios Theodorou, Ioannis Politis
The urokinase-plasminogen activator (u-PA), its receptor (u-PAR) and the inhibitors of u-PA (PAI-1 and PAI-2) provide a multi-molecular system in leucocytes that exerts pleiotropic functions influencing the development of inflammatory and immune responses. The objective of the present study was to examine the ability of water soluble extracts (WSE) obtained from traditional Greek yoghurt made from bovine or ovine milk to modulate the expression of u-PA, u-PAR, PAI-1 and PAI-2 in ovine monocytes and neutrophils...
August 2016: Journal of Dairy Research
https://www.readbyqxmd.com/read/27598010/fibrinolysis-parameters-in-sudanese-women-with-severe-preeclampsia
#7
Husham O Elzein, Abdel Rahim M Muddathir, Mohammed Rida, Duria A Rayis, Elhassan M Elhassan, Ishag Adam
BACKGROUND: Although preeclampsia remains a major cause of maternal and fetal morbidity and mortality, its pathogenesis is not fully understood. Coagulation and fibrinolysis changes were suggested to have a role in the pathogenesis of preeclampsia. OBJECTIVES: A case-control study was conducted in Medani Hospital, Sudan, to investigate thrombin-activatable fibrinolysis inhibitor (TAFI) and plasminogen-activated inhibitor (PAI) in women with severe preeclampsia. Obstetrics and medical history was gathered using questionnaire...
September 6, 2016: Hypertension in Pregnancy
https://www.readbyqxmd.com/read/27554634/pzp-and-pai-2-structurally-diverse-functionally-similar-pregnancy-proteins
#8
Amy R Wyatt, Jordan H Cater, Marie Ranson
Pregnancy zone protein (PZP) and plasminogen activator inhibitor type 2 (PAI-2) are two multifunctional proteins that are elevated in normal pregnancy and numerous other inflammatory states. Both proteins were originally identified as protease inhibitors, but current evidence supports the notion that they may also function as modulators of T-helper cells and/or extracellular chaperones. Exacerbated inflammation, fibrinolytic disturbances and misfolded proteins are all implicated in the pathology of preeclampsia, a leading cause of maternal and foetal mortality and morbidity...
October 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27412942/-xinfeng-capsule-improves-hypercoagulative-state-by-inhibiting-mir-155-nf-%C3%AE%C2%BAb-signaling-pathway-in-patients-with-active-ankylosing-spondylitis
#9
Li Fang, Jian Liu, Lei Wan, Fubing Zhu, Bing Tan, Pingheng Zhang
Objective To observe the effect of Xinfeng capsule (XFC) on miR-155, nuclear factor kappa B (NF-κB) signal pathway, and indexes related to hypercoagulative state in patients with active ankylosing spondylitis (AS), and investigate the possible mechanism. Methods Fifty-six cases in active AS were randomly divided into XFC group and sulfasalazine (SASP) group. All cases in the XFC group took three capsules three times daily for twelve consecutive weeks. The ones in the SASP group took four tablets two times daily for twelve consecutive weeks...
August 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27345498/is-there-a-role-for-urokinase-type-plasminogen-activator-inhibitors-as-maintenance-therapy-in-patients-with-ovarian-cancer
#10
P A van Dam, A Coelho, C Rolfo
There is abundant evidence that the urokinase-type plasminogen activator (uPA), its inhibitors PAI-1 and PAI-2 (plasminogen activator inhibitor type-1 and type-2) and its cells surface receptor (uPA-R, CD87) play a fundamental role in tumor invasion and metastasis and are of significant prognostic significance for many tumor types. We performed a systematic Med-line search on uPA, PAI, uPA-R and (epithelial) ovarian cancer (EOC). The majority of malignant EOC specimens show moderate to strong immunostating of tumor and stromal cells...
June 20, 2016: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27295389/pai-2-serpinb2-inhibits-proteolytic-activity-in-a-p-gingivalis-dominated-multispecies-bacterial-consortium
#11
Jessica Neilands, Floris J Bikker, Bertil Kinnby
OBJECTIVE: The aim of this study was to investigate the ability of the serine protease inhibitor plasminogen activator inhibitor type 2 (PAI-2/Serpin B2) to inhibit proteases produced by a multispecies bacterial consortium in vitro. BACKGROUND: Gingival and periodontal inflammation is associated with an increased flow of protein-rich gingival fluid. This nutritional change in the microenvironment favors bacteria with a proteolytic phenotype, triggering inflammation and associated tissue breakdown...
May 27, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27284570/data-in-support-of-a-central-role-of-plasminogen-activator-inhibitor-2-polymorphism-in-recurrent-cardiovascular-disease-risk-in-the-setting-of-high-hdl-cholesterol-and-c-reactive-protein-using-bayesian-network-modeling
#12
James P Corsetti, Peter Salzman, Dan Ryan, Arthur J Moss, Wojciech Zareba, Charles E Sparks
Data is presented that was utilized as the basis for Bayesian network modeling of influence pathways focusing on the central role of a polymorphism of plasminogen activator inhibitor-2 (PAI-2) on recurrent cardiovascular disease risk in patients with high levels of HDL cholesterol and C-reactive protein (CRP) as a marker of inflammation, "Influences on Plasminogen Activator Inhibitor-2 Polymorphism-Associated Recurrent Cardiovascular Disease Risk in Patients with High HDL Cholesterol and Inflammation" (Corsetti et al...
September 2016: Data in Brief
https://www.readbyqxmd.com/read/27174532/influences-on-plasminogen-activator-inhibitor-2-polymorphism-associated-recurrent-cardiovascular-disease-risk-in-patients-with-high-hdl-cholesterol-and-inflammation
#13
James P Corsetti, Peter Salzman, Dan Ryan, Arthur J Moss, Wojciech Zareba, Charles E Sparks
BACKGROUND AND AIMS: Evidence continues to accumulate that athero-protective effects of high-density lipoprotein (HDL) depend to some degree on effective HDL functionality and that such functionality can become degraded in the setting of chronic inflammation. To investigate this issue, we have studied a group of post-myocardial infarction patients with high levels of C-reactive protein as an indicator of chronic inflammation and with concurrently high levels of HDL cholesterol. For these patients we have demonstrated high-risk for recurrent cardiac events as well as a strong association of risk with a polymorphism of the gene (SERPINB2) for plasminogen activator inhibitor-2 (PAI-2) presumptively reflective of an important role for fibrinolysis in risk...
July 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27144072/single-versus-multi-visit-endodontic-treatment-of-teeth-with-apical-periodontitis-an-in-vivo-study-with-1-year-evaluation
#14
G S Gill, A C Bhuyan, C Kalita, L Das, R Kataki, D Bhuyan
BACKGROUND: Apical periodontitis (AP) is one of the most prevalent diseases of the teeth. Treatment of AP is based on the removal of the cause, i.e., bacteria from the root canals. Achievement of adequate bacterial eradication in one appointment treatment remains a controversy. AIM: This prospective study was conducted with the objective to compare the periapical healing of teeth with AP treated in (a) single visit versus (b) two visits, either with or without Vitapex as an intracanal medicament...
January 2016: Annals of Medical and Health Sciences Research
https://www.readbyqxmd.com/read/27122455/-expression-of-pai-2-mrna-in-peripheral-blood-leucocytes-and-regulation-by-sgc-activator-in-pulmonary-hypertension
#15
L H Zou, S Zhang, X M Xu, F Xiao, Z G Zhai
OBJECTIVE: To investigate the mRNA expression level of plasminogen activator inhibitor-2 (PAI-2) in peripheral blood leucocytes and regulation by soluble guanylate cyclase (sGC) activator in pulmonary hypertension. METHODS: The human pulmonary arterial smooth muscle cells were treated with sGC activator Cinaciguat. The mRNA and protein expression levels of PAI-2 were detected with Real-time PCR and Western blot. The fresh blood samples of 8 patients with pulmonary arterial hypertension (PAH) (collected at the China-Japan Friendship Hospital from November 2014 to March 2015), 16 patients with chronic thromboembolic pulmonary hypertension (CTEPH) (collected at the China-Japan Friendship Hospital from November 2014 to March 2015), 24 age- and gender- matched healthy controls (collected at Beijing Hospital in March 2015) were treated with Cinaciguat for 8 hours...
April 26, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/26949763/long-term-outcome-of-mta-apexification-in-teeth-with-open-apices
#16
Katharina Bücher, Franziska Meier, Christian Diegritz, Christoph Kaaden, Reinhard Hickel, Jan Kühnisch
OBJECTIVE: This retrospective study aimed to collect information about the long-term outcome of apexification treatment with mineral trioxide aggregate (MTA) of teeth with open apices. METHOD AND MATERIALS: A total of 98 teeth in 79 patients (m:f = 1:1.3) who had completed endodontic apexification treatment with MTA between September 2005 and January 2014 at a university dental clinic were considered. Both initial treatments and retreatments of former root canal treatments other than apexification were included...
2016: Quintessence International
https://www.readbyqxmd.com/read/26901847/radiotherapy-diagnostic-biomarkers-in-radioresistant-human-h460-lung-cancer-stem-like-cells
#17
Hong Shik Yun, Jeong-Hwa Baek, Ji-Hye Yim, Hong-Duck Um, Jong Kuk Park, Jie-Young Song, In-Chul Park, Jae-Sung Kim, Su-Jae Lee, Chang-Woo Lee, Sang-Gu Hwang
Tumor cell radioresistance is a major contributor to radiotherapy failure, highlighting the importance of identifying predictive biomarkers for radioresistance. In this work, we established a radioresistant H460 (RR-H460) cell line from parental radiosensitive H460 lung cancer cells by exposure to fractionated radiation. The radiation-resistant, anti-apoptotic phenotype of RR-H460 cell lines was confirmed by their enhanced clonogenic survival and increased expression of the radioresistance genes Hsp90 and Her-3...
2016: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/26667094/the-urokinase-type-plasminogen-activator-upa-system-as-a-biomarker-and-therapeutic-target-in-human-malignancies
#18
Shih-Chi Su, Chiao-Wen Lin, Wei-En Yang, Wen-Lang Fan, Shun-Fa Yang
INTRODUCTION: The urokinase plasminogen activator (uPA) system, comprising the serine protease uPA, its cognate receptor, uPAR, and two endogenous inhibitors, plasminogen activator inhibitor-1 (PAI-1) and plasminogen activator inhibitor-2 (PAI-2), is a key player in the break-down of extracellular matrix (ECM) and basement membrane. Elevated expression of uPA and uPAR is observed in numerous cancer types and associated with poor prognosis. AREAS COVERED: In addition to the aberrant expression during tumor development, the components of uPA system are functionally involved in various processes that are prerequisite for cancer progression...
2016: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/26573152/variant-of-pai-2-gene-is-associated-with-coronary-artery-disease-and-recurrent-coronary-event-risk-in-chinese-han-population
#19
Xia Li, Jun-Yi Luo, Lei Zhang, Yi-Ning Yang, Xiang Xie, Fen Liu, Bang-Dang Chen, Yi-Tong Ma
BACKGROUND: Plasminogen activator inhibitor -2 (PAI-2) is an important molecular that plays a crucial role in vascular homeostasis and constitutes a critical response mechanism to cardiovascular injury, such as atherosclerosis, coronary artery disease (CAD). METHODS: The aim of the current study was to explore the association between the variants in PAI-2 gene and CAD and its prognoses. The three variants (rs8093048, rs9946657, rs9320032) of the PAI-2 gene were detected in 407 patients with CAD and 518 control subjects...
2015: Lipids in Health and Disease
https://www.readbyqxmd.com/read/26561613/periconceptional-multiple-micronutrient-supplementation-and-placental-function-in-rural-gambian-women-a-double-blind-randomized-placebo-controlled-trial
#20
RANDOMIZED CONTROLLED TRIAL
Stephen Owens, Ruchi Gulati, Anthony J Fulford, Fatou Sosseh, Fiona C Denison, Bernard J Brabin, Andrew M Prentice
BACKGROUND: Maternal micronutrient deficiencies are commonly associated with clinical indicators of placental dysfunction. OBJECTIVE: We tested the hypothesis that periconceptional multiple-micronutrient supplementation (MMS) affects placental function. DESIGN: We conducted a double-blind, randomized, placebo-controlled trial of MMS in 17- to 45-y-old Gambian women who were menstruating regularly and within the previous 3 mo. Eligible subjects were pre-randomly assigned to supplementation with the UNICEF/WHO/United Nations University multiple micronutrient preparation (UNIMMAP) or placebo on recruitment and until they reached their first antenatal check-up or for 1 y if they failed to conceive...
December 2015: American Journal of Clinical Nutrition
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