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Araceli Garcia-Ac, Sung Vo Duy, Sébastien Sauvé, Florina Moldovan, V Gaëlle Roullin, Xavier Banquy
A method to explore the stability of two anti-inflammatory peptides in human synovial fluid (HSF) has been developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The two peptides are BQ123 Cyclo(-D-Trp-D-Asp-L-Pro-D-Val-L-Leu, Mw = 610.7) and R-954 (AcOrn[Oic2 , (αMe)Phe5 , DβNal7 , Ile8 ]desArg9 -bradykinin, Mw = 1194.4). Human synovial fluid samples were analyzed after a protein precipitation step with acetonitrile and dilution with mobile phase. DMSO was used as anti-adsorptive agent...
August 15, 2018: Talanta
Tatjana Boroja, Jelena Katanić, Gvozden Rosić, Dragica Selaković, Jovana Joksimović, Danijela Mišić, Vesna Stanković, Nemanja Jovičić, Vladimir Mihailović
The aim of our study was to examine the potential ameliorating effect of the methanolic extract of Satureja hortensis L. (summer savory) aerial parts against cisplatin-induced oxidative damage in renal, hepatic, and testicular tissues. S. hortensis methanol extract at the doses of 50, 100 and 200 mg/kg of body weight were orally administered to Wistar rats once daily for 10 days. Toxicity was induced by intraperitoneal injection of a single dose of cisplatin (7.5 mg/kg of body weight) on the 5th day of the experiment...
May 7, 2018: Food and Chemical Toxicology
Andrew Sawers, Brian J Hafner
OBJECTIVE: Challenging clinical balance tests are needed to expose balance deficits in lower-limb prost-hesis users. This study examined whether narrowing beam-walking could overcome conceptual and practical limitations identified in fixed-width beam-walking. DESIGN: Cross-sectional. PARTICIPANTS: Unilateral lower-limb prosthesis users. METHODS: Participants walked 10 times along a low, narrowing beam. Performance was quantified using the normalized distance walked...
April 4, 2018: Journal of Rehabilitation Medicine
Rong Li, Luqi Yang, Pengyun Zhang, Yangdan Luo, Pengjie Zhang, Yongqing Gao
A method was developed for the simultaneous determination of 24 tranquillizer drugs in fish and fishery products using ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). The samples were extracted with acetonitrile. Then, the extracts were concentrated, dissolved by 50% (v/v) methanol aqueous solution, cleaned up with hexane saturated by acetonitrile. The separation was performed on an ACQUITY UPLC® BEH C18 column (100 mm×2...
February 8, 2018: Se Pu, Chinese Journal of Chromatography
Xianchen Zhang, Rong Li, Pengjie Zhang, Xia Wu, Hongbo Hua, Luqi Yang, Junwen Lu, Yutang Rong
An ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Orbitrap HRMS) method was developed for the rapid screening of 25 drug residues in aquatic products by the application of a novel enhanced matrix removal of lipids (EMR-Lipid). The samples were extracted with acetonitrile, cleaned-up with EMR-Lipid, and salted out with 3.0 g sodium chloride and 3.0 g sodium sulfate. The separation of the 25 drug residues was performed on an ACQUITY UPLC BEH C18 column (100 mm×2...
February 8, 2018: Se Pu, Chinese Journal of Chromatography
Alan Y Chiang, Brian D Guth, Michael K Pugsley, C Michael Foley, Jennifer M Doyle, Michael J Engwall, John E Koerner, Stanley T Parish, R Dustan Sarazan
INTRODUCTION: The sensitivity of a given test to detect a treatment-induced effect in a variable of interest is intrinsically related to the variability of that variable observed without treatment and the number of observations made in the study (i.e. number of animals). To evaluate test sensitivity to detect drug-induced changes in myocardial contractility using the variable LVdP/dtmax , a HESI-supported consortium designed and conducted studies in chronically instrumented, conscious dogs using telemetry...
March 2, 2018: Journal of Pharmacological and Toxicological Methods
Jiying Pei, Cheng-Chih Hsu, Kefu Yu, Yinghui Wang, Guangming Huang
Electrospray ionization mass spectrometry (ESI-MS) is one of the most prevalent techniques used to monitor protein/peptide oxidation induced by reactive oxygen species (ROSs). However, both corona discharge (CD) and electrochemistry (EC) can also lead to protein/peptide oxidation during ESI. Because the two types of oxidation occur almost simultaneously, determining the extent to which the two pathways contribute to protein/peptide oxidation is difficult. Herein, a time-resolved method was introduced to identify and differentiate CD- and EC-induced oxidation...
June 29, 2018: Analytica Chimica Acta
Andrea Speltini, Francesca Merlo, Federica Maraschi, Michela Sturini, Matteo Contini, Nicola Calisi, Antonella Profumo
Pristine humic acids (HAs) were thermally condensed onto silica microparticles by a one-pot, inexpensive and green preparation route obtaining a mixed-mode sorbent (HA-C@silica) with good sorption affinity for glucocorticoids (GCs). The carbon-based material, characterized by various techniques, was indeed applied as the sorbent for fixed-bed solid-phase extraction of eight GCs from river water and wastewater treatment plant effluent, spiked at different concentration levels in the range 1-400 ng L-1 . After sample extraction, the target analytes were simultaneously and quantitatively eluted in a single fraction of methanol, achieving enrichment factor 4000 and 1000 in river water and wastewater effluent, respectively...
March 9, 2018: Journal of Chromatography. A
D C Bobori, Ch Ntislidou, O Petriki, I Chronis, I Kagalou, M Lazaridou
The role of benthic macroinvertebrate and fish communities for assessing the ecological quality of an artificial re-constructed, after 50 years of dryness, Mediterranean water body (Karla Reservoir, Greece) is presented. Moreover, we provide knowledge on the structure of the biological communities and their functioning role, for inspiring feature actions that will contribute to biodiversity protection and ecosystem services. Water (physicochemical parameters), benthic macroinvertebrates, and fish were monitored during a 2-year survey (2013-2015) in Karla and Kalamaki reservoirs and the inflowing ditches...
February 1, 2018: Environmental Monitoring and Assessment
Yongtao Liu, Linxue Yu, Zhenyue Wang, Qiuhong Yang, Jing Dong, Yibin Yang, Xiaohui Ai
A method was established for the simultaneous determination of seven avermectin (AVMs) residues, such as avermectin, ivermectin, doramectin, selamectin, eprinomectin, moxidectin and emamectin, in aquatic products using modified QuEChERS and high-performance liquid chromatography -tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with 0.2% (v/v) ammoniate acetonitrile, and then 3 g of anhydrous magnesium sulfate and 2 g of anhydrous sodium sulfate were added to remove moisture and precipitate proteins...
December 8, 2017: Se Pu, Chinese Journal of Chromatography
Cui-Cui Wang, Maria Sulli, Da-Qi Fu
Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is currently unknown. Chlorophyll and carotenoid biosynthesis depend on light signal transduction and share some intermediate metabolites with the glycoalkaloid biosynthetic pathway. Here, we used virus-induced gene silencing to silence genes encoding phytoene desaturase (PDS) and magnesium chelatase (CHLI and CHLH) to reduce chlorophyll and carotenoid levels in eggplant leaves...
2017: PloS One
Reneta Gevrenova, Ventzislav Bardarov, Krum Bardarov, Laurence Voutquenne-Nazabadioko, Max Henry
INTRODUCTION: Roots of Gypsophila trichotoma Wend. (Caryophyllaceae) are rich sources of glucuronide oleanane-type triterpenoid carboxylic acid 3,28-O-bidesmosides (GOTCABs). These saponins have been reported to possess synergistic cytotoxicity in combination with type I ribosome-inactivating protein saporin. OBJECTIVE: To develop ultra high-performance liquid chromatography - electrospray ionisation/high resolution mass spectrometry (UHPLC-ESI/HRMS) acquisition strategy for the recognition of Gypsophila GOTCAB saponins...
December 4, 2017: Phytochemical Analysis: PCA
Chenxi Liao, Wei Liu, Jialing Zhang, Wenming Shi, Xueying Wang, Jiao Cai, Zhijun Zou, Rongchun Lu, Chanjuan Sun, Heng Wang, Chen Huang, Zhuohui Zhao
Exposure to household phthalates has been reported to have adverse effects on children's health. In this paper, we used phthalate metabolites in the first morning urine as indicators of household phthalate exposures and examined their associations with residential characteristics, lifestyles and dietary habits among young children. During 2013-2014, we collected morning urines from children aged 5-10years in Shanghai, China and obtained the related information about analyzed factors in this study by questionnaires...
March 2018: Science of the Total Environment
Nicole Robert
Unsuccessful nursing students have a great impact on nursing programs. The purpose of this study was to determine the relationships among preadmission HESI A2 (Health Education Systems, Incorporated Admission Assessment) score, science and math course grades, program completion, and NCLEX-RN success in an associate degree nursing program using a retrospective, correlational research design. A statistically significant relationship was found between preadmission examination scores and successful NCLEX-RN passage on first attempt...
January 2018: Nursing Education Perspectives
Andrea Speltini, Federica Maraschi, Michela Sturini, Matteo Contini, Antonella Profumo
This work shows a novel analytical method for the simultaneous extraction of environmental emerging contaminants as benzenesulfonamides (BSAs), benzotriazoles (BTRs), and benzothiazoles (BTs) from water samples. Pristine multi-walled carbon nanotubes (MWCNTs), not yet tested for such analytes, are here employed as the sorbent phase for dispersive solid-phase extraction (d-SPE) followed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-HESI-MS/MS). Quantitative sorption is gained by treating 50 mL sample with 100 mg MWCNTs (2 g L-1 ) in 10 min contact, both in tap and raw river water...
November 2017: Analytical and Bioanalytical Chemistry
Adrian Rodriguez, Rosa Maria Gomila, Gabriel Martorell, Antonia Costa-Bauza, Felix Grases
Quantitative analysis of relevant metabolites in biofluids such as urine is often a tedious procedure, since it usually requires extraction, purification or preconcentration. For instance, in the analysis of methylxanthines in urine, a solid-phase extraction is often required. In the current work, a rapid and highly sensitive "dilute-and-shoot" method combining ultra-high-performance liquid chromatography and high-resolution mass spectrometry (UHPLC/HRMS) was validated for urinary determination of twelve analytes: uric acid, hypoxanthine, xanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, theophylline, theobromine, paraxanthine and caffeine...
November 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ravi Prakash, Tara Beattie, Prakash Javalkar, Parinita Bhattacharjee, Satyanarayana Ramanaik, Raghavendra Thalinja, Srikanta Murthy, Calum Davey, James Blanchard, Charlotte Watts, Martine Collumbien, Stephen Moses, Lori Heise, Shajy Isac
Secondary education among lower caste adolescent girls living in rural Karnataka, South India, is characterized by high rates of school drop-out and absenteeism. A cross-sectional baseline survey (N=2275) was conducted in 2014 as part of a cluster-randomized control trial among adolescent girls (13-14 year) and their families from marginalized communities in two districts of north Karnataka. Bivariate and multivariate logistic regression models were used. Overall, 8.7% girls reported secondary school dropout and 8...
September 29, 2017: Journal of Adolescence
Hanna Niemikoski, Martin Söderström, Paula Vanninen
A new method has been developed to determine oxidation products of three chemical warfare agent (CWA) related phenylarsenic compounds from marine biota samples by a liquid chromatography-heated electrospray ionization/tandem mass spectrometry (LC-HESI/MS/MS). The target chemicals were oxidation products of Adamsite (DM[ox]), Clark I (DPA[ox]), and triphenylarsine (TPA[ox]). Method was validated within the concentration range of 1-5, 0.2-5, and 0.2-5 ng/g for DM[ox], DPA[ox], and TPA[ox], respectively. The method was linear, precise and accurate...
October 17, 2017: Analytical Chemistry
Tanya Johnson, Bonnie Sanderson, Chih-Hsuan Wang, Francine Parker
BACKGROUND: The purpose of this study was to identify variables associated with scores achieved on the Health Education Systems, Inc. (HESI) exit examination and successful first-time NCLEX-RN(®) pass rates. METHOD: A retrospective descriptive study examined the administrative data of 211 baccalaureate nursing students. RESULTS: Students who completed the program in sequence and scored higher in certain HESI course examinations were more likely to have a better performance on the HESI exit examination...
September 1, 2017: Journal of Nursing Education
Sonia Bustamante, Robert B Gilchrist, Dulama Richani
A liquid chromatography coupled to heated electrospray ionization/tandem mass spectrometry (LC-HESI-MS/MS) method was developed for the simultaneous quantitative analysis of low nanomolar level adenine nucleotides AMP, ADP, ATP, cyclic AMP (cAMP), and the nucleoside adenosine. For analyte retention and separation, reverse phase chromatography using porous graphitic carbon (PGC) was employed as it provided full resolution. The erratic chromatographic behaviour characteristic of PGC, including deterioration of analyte resolution and increased peak tailing (leading to decreased sensitivity), was mitigated by incorporating acidic equilibration within runs using a quaternary gradient...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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