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Alan Y Chiang, Brian D Guth, Michael K Pugsley, C Michael Foley, Jennifer M Doyle, Michael J Engwall, John E Koerner, Stanley T Parish, R Dustan Sarazan
INTRODUCTION: The sensitivity of a given test to detect a treatment-induced effect in a variable of interest is intrinsically related to the variability of that variable observed without treatment and the number of observations made in the study (i.e. number of animals). To evaluate test sensitivity to detect drug-induced changes in myocardial contractility using the variable LVdP/dtmax , a HESI-supported consortium designed and conducted studies in chronically instrumented, conscious dogs using telemetry...
March 2, 2018: Journal of Pharmacological and Toxicological Methods
Jiying Pei, Cheng-Chih Hsu, Kefu Yu, Yinghui Wang, Guangming Huang
Electrospray ionization mass spectrometry (ESI-MS) is one of the most prevalent techniques used to monitor protein/peptide oxidation induced by reactive oxygen species (ROSs). However, both corona discharge (CD) and electrochemistry (EC) can also lead to protein/peptide oxidation during ESI. Because the two types of oxidation occur almost simultaneously, determining the extent to which the two pathways contribute to protein/peptide oxidation is difficult. Herein, a time-resolved method was introduced to identify and differentiate CD- and EC-induced oxidation...
June 29, 2018: Analytica Chimica Acta
Andrea Speltini, Francesca Merlo, Federica Maraschi, Michela Sturini, Matteo Contini, Nicola Calisi, Antonella Profumo
Pristine humic acids (HAs) were thermally condensed onto silica microparticles by a one-pot, inexpensive and green preparation route obtaining a mixed-mode sorbent (HA-C@silica) with good sorption affinity for glucocorticoids (GCs). The carbon-based material, characterized by various techniques, was indeed applied as the sorbent for fixed-bed solid-phase extraction of eight GCs from river water and wastewater treatment plant effluent, spiked at different concentration levels in the range 1-400 ng L-1. After sample extraction, the target analytes were simultaneously and quantitatively eluted in a single fraction of methanol, achieving enrichment factor 4000 and 1000 in river water and wastewater effluent, respectively...
February 5, 2018: Journal of Chromatography. A
D C Bobori, Ch Ntislidou, O Petriki, I Chronis, I Kagalou, M Lazaridou
The role of benthic macroinvertebrate and fish communities for assessing the ecological quality of an artificial re-constructed, after 50 years of dryness, Mediterranean water body (Karla Reservoir, Greece) is presented. Moreover, we provide knowledge on the structure of the biological communities and their functioning role, for inspiring feature actions that will contribute to biodiversity protection and ecosystem services. Water (physicochemical parameters), benthic macroinvertebrates, and fish were monitored during a 2-year survey (2013-2015) in Karla and Kalamaki reservoirs and the inflowing ditches...
February 1, 2018: Environmental Monitoring and Assessment
Yongtao Liu, Linxue Yu, Zhenyue Wang, Qiuhong Yang, Jing Dong, Yibin Yang, Xiaohui Ai
A method was established for the simultaneous determination of seven avermectin (AVMs) residues, such as avermectin, ivermectin, doramectin, selamectin, eprinomectin, moxidectin and emamectin, in aquatic products using modified QuEChERS and high-performance liquid chromatography -tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with 0.2% (v/v) ammoniate acetonitrile, and then 3 g of anhydrous magnesium sulfate and 2 g of anhydrous sodium sulfate were added to remove moisture and precipitate proteins...
December 8, 2017: Se Pu, Chinese Journal of Chromatography
Cui-Cui Wang, Maria Sulli, Da-Qi Fu
Glycoalkaloids are toxic compounds that are synthesized by many Solanum species. Glycoalkaloid biosynthesis is influenced by plant genetic and environmental conditions. Although many studies have shown that light is an important factor affecting glycoalkaloid biosynthesis, the specific mechanism is currently unknown. Chlorophyll and carotenoid biosynthesis depend on light signal transduction and share some intermediate metabolites with the glycoalkaloid biosynthetic pathway. Here, we used virus-induced gene silencing to silence genes encoding phytoene desaturase (PDS) and magnesium chelatase (CHLI and CHLH) to reduce chlorophyll and carotenoid levels in eggplant leaves...
2017: PloS One
Reneta Gevrenova, Ventzislav Bardarov, Krum Bardarov, Laurence Voutquenne-Nazabadioko, Max Henry
INTRODUCTION: Roots of Gypsophila trichotoma Wend. (Caryophyllaceae) are rich sources of glucuronide oleanane-type triterpenoid carboxylic acid 3,28-O-bidesmosides (GOTCABs). These saponins have been reported to possess synergistic cytotoxicity in combination with type I ribosome-inactivating protein saporin. OBJECTIVE: To develop ultra high-performance liquid chromatography - electrospray ionisation/high resolution mass spectrometry (UHPLC-ESI/HRMS) acquisition strategy for the recognition of Gypsophila GOTCAB saponins...
December 4, 2017: Phytochemical Analysis: PCA
Chenxi Liao, Wei Liu, Jialing Zhang, Wenming Shi, Xueying Wang, Jiao Cai, Zhijun Zou, Rongchun Lu, Chanjuan Sun, Heng Wang, Chen Huang, Zhuohui Zhao
Exposure to household phthalates has been reported to have adverse effects on children's health. In this paper, we used phthalate metabolites in the first morning urine as indicators of household phthalate exposures and examined their associations with residential characteristics, lifestyles and dietary habits among young children. During 2013-2014, we collected morning urines from children aged 5-10years in Shanghai, China and obtained the related information about analyzed factors in this study by questionnaires...
November 6, 2017: Science of the Total Environment
Nicole Robert
Unsuccessful nursing students have a great impact on nursing programs. The purpose of this study was to determine the relationships among preadmission HESI A2 (Health Education Systems, Incorporated Admission Assessment) score, science and math course grades, program completion, and NCLEX-RN success in an associate degree nursing program using a retrospective, correlational research design. A statistically significant relationship was found between preadmission examination scores and successful NCLEX-RN passage on first attempt...
November 9, 2017: Nursing Education Perspectives
Andrea Speltini, Federica Maraschi, Michela Sturini, Matteo Contini, Antonella Profumo
This work shows a novel analytical method for the simultaneous extraction of environmental emerging contaminants as benzenesulfonamides (BSAs), benzotriazoles (BTRs), and benzothiazoles (BTs) from water samples. Pristine multi-walled carbon nanotubes (MWCNTs), not yet tested for such analytes, are here employed as the sorbent phase for dispersive solid-phase extraction (d-SPE) followed by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-HESI-MS/MS). Quantitative sorption is gained by treating 50 mL sample with 100 mg MWCNTs (2 g L(-1)) in 10 min contact, both in tap and raw river water...
October 25, 2017: Analytical and Bioanalytical Chemistry
Adrian Rodriguez, Rosa Maria Gomila, Gabriel Martorell, Antonia Costa-Bauza, Felix Grases
Quantitative analysis of relevant metabolites in biofluids such as urine is often a tedious procedure, since it usually requires extraction, purification or preconcentration. For instance, in the analysis of methylxanthines in urine, a solid-phase extraction is often required. In the current work, a rapid and highly sensitive "dilute-and-shoot" method combining ultra-high-performance liquid chromatography and high-resolution mass spectrometry (UHPLC/HRMS) was validated for urinary determination of twelve analytes: uric acid, hypoxanthine, xanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, theophylline, theobromine, paraxanthine and caffeine...
November 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ravi Prakash, Tara Beattie, Prakash Javalkar, Parinita Bhattacharjee, Satyanarayana Ramanaik, Raghavendra Thalinja, Srikanta Murthy, Calum Davey, James Blanchard, Charlotte Watts, Martine Collumbien, Stephen Moses, Lori Heise, Shajy Isac
Secondary education among lower caste adolescent girls living in rural Karnataka, South India, is characterized by high rates of school drop-out and absenteeism. A cross-sectional baseline survey (N=2275) was conducted in 2014 as part of a cluster-randomized control trial among adolescent girls (13-14 year) and their families from marginalized communities in two districts of north Karnataka. Bivariate and multivariate logistic regression models were used. Overall, 8.7% girls reported secondary school dropout and 8...
September 29, 2017: Journal of Adolescence
Hanna Niemikoski, Martin Söderström, Paula Vanninen
A new method has been developed to determine oxidation products of three chemical warfare agent (CWA) related phenylarsenic compounds from marine biota samples by a liquid chromatography-heated electrospray ionization/tandem mass spectrometry (LC-HESI/MS/MS). The target chemicals were oxidation products of Adamsite (DM[ox]), Clark I (DPA[ox]), and triphenylarsine (TPA[ox]). Method was validated within the concentration range of 1-5, 0.2-5, and 0.2-5 ng/g for DM[ox], DPA[ox], and TPA[ox], respectively. The method was linear, precise and accurate...
October 17, 2017: Analytical Chemistry
Tanya Johnson, Bonnie Sanderson, Chih-Hsuan Wang, Francine Parker
BACKGROUND: The purpose of this study was to identify variables associated with scores achieved on the Health Education Systems, Inc. (HESI) exit examination and successful first-time NCLEX-RN(®) pass rates. METHOD: A retrospective descriptive study examined the administrative data of 211 baccalaureate nursing students. RESULTS: Students who completed the program in sequence and scored higher in certain HESI course examinations were more likely to have a better performance on the HESI exit examination...
September 1, 2017: Journal of Nursing Education
Sonia Bustamante, Robert B Gilchrist, Dulama Richani
A liquid chromatography coupled to heated electrospray ionization/tandem mass spectrometry (LC-HESI-MS/MS) method was developed for the simultaneous quantitative analysis of low nanomolar level adenine nucleotides AMP, ADP, ATP, cyclic AMP (cAMP), and the nucleoside adenosine. For analyte retention and separation, reverse phase chromatography using porous graphitic carbon (PGC) was employed as it provided full resolution. The erratic chromatographic behaviour characteristic of PGC, including deterioration of analyte resolution and increased peak tailing (leading to decreased sensitivity), was mitigated by incorporating acidic equilibration within runs using a quaternary gradient...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Fleur Gaudette, Adjia Hamadjida, Dominique Bédard, Stephen G Nuara, Francis Beaudry, Philippe Huot
LY-354,740 (eglumegad) is a selective and potent agonist of the metabotropic glutamate group II receptors (mGluR2,3) that has already entered clinical trials as a potential anti-psychotic agent and therefore has well-documented pharmacokinetic (PK), safety and tolerability profiles in human. Whereas its development as an anti-psychotic agent has not been pursued, LY-354,740 may have potential in other neuroscience-related fields, notably anxiety and neuro-protection. The common marmoset is a small primate that has long been used in neuroscience...
September 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Carsten Müller, David Gehlen, Cornelia Blaich, Domenik Prozeller, Blasius Liss, Thomas Streichert, Martin H J Wiesen
BACKGROUND: A fast and easy-to-use liquid chromatography-tandem mass spectrometry method for the determination and quantification of 6 triazoles [fluconazole (FLZ), isavuconazole (ISZ), itraconazole (ITZ), hydroxy-itraconazole (OH-ITZ), posaconazole (PSZ), and voriconazole (VRZ)] in human plasma and serum was developed and validated for therapeutic drug monitoring. METHODS: Sample preparation was based on protein precipitation with acetonitrile and subsequent centrifugation...
October 2017: Therapeutic Drug Monitoring
Jayasree Leela Devi, Paul Zahra, John H Vine, Ted Whittem
The doping of greyhound dogs with testosterone is done in an attempt to improve their athletic performance, but such doping cannot easily be confirmed especially in male dogs owing to the natural presence of endogenous testosterone. As testosterone is usually administered as its esters their direct detection in hair would provide confirmatory evidence of the administration of a pharmaceutical product. This paper demonstrates that the use of a liquid chromatography-high resolution mass spectrometry method with heated electrospray ionisation (HESI) combined with the use of amino SPE cartridges for sample clean up, is suitable for the sensitive determination of propionate, phenyl propionate, isocaproate, decanoate and enanthate esters of testosterone in greyhound hair...
June 28, 2017: Drug Testing and Analysis
Jasna Bosnjak-Neumuller, Milena Radakovic, Ninoslav Djelic, Branka Vukovic-Gacic, Zora Dajic Stevanovic, Stoimir Kolarevic, Danijela Misic, Milan Stankovic, Jelena Knezevic-Vukcevicc, Biljana Spremo-Potparevic, Zoran Stanimirovic
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex...
May 2017: Pakistan Journal of Pharmaceutical Sciences
Jasna Bošnjak-Neumüller, Milena Radaković, Ninoslav Djelić, Branka Vuković-Gačić, Zora Dajić Stevanović, Stoimir Kolarević, Danijela Mišić, Milan Stanković, Jelena Knežević-Vukčević, Biljana Spremo-Potparević, Zoran Stanimirović
The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex...
March 2017: Pakistan Journal of Pharmaceutical Sciences
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