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Holographic microscopy

Gaël Nardin, Tristan Colomb, Yves Emery, Christophe Moser
We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a 'pulse-shaping' geometry, the spatial light modulator provides a versatile control over the bandwidth and wavelength of the light source. The control of these properties enables adaptation to various experimental conditions. As a first application, we show that the source bandwidth can be adapted to the off-axis geometry to provide quantitative phase imaging over the whole field of view...
November 28, 2016: Optics Express
Yair Rivenson, Yichen Wu, Hongda Wang, Yibo Zhang, Alborz Feizi, Aydogan Ozcan
High-resolution imaging of densely connected samples such as pathology slides using digital in-line holographic microscopy requires the acquisition of several holograms, e.g., at >6-8 different sample-to-sensor distances, to achieve robust phase recovery and coherent imaging of specimen. Reducing the number of these holographic measurements would normally result in reconstruction artifacts and loss of image quality, which would be detrimental especially for biomedical and diagnostics-related applications...
November 30, 2016: Scientific Reports
Chaojie Ma, Jianglei Di, Jiwei Zhang, Ying Li, Teli Xi, Enpu Li, Jianlin Zhao
We propose a method for simultaneously measuring dynamic changes of the refractive index distribution and surface topography, which integrates the transmission and reflection digital holographic microscopy based on polarization and angular multiplexing techniques. The complex amplitudes of the transmitted and reflected object waves can be simultaneously retrieved. The phase information of the reflected object wave is directly used to determine the topography of the specimen which corresponds to its physical thickness...
November 20, 2016: Applied Optics
F Saglimbeni, S Bianchi, G Gibson, R Bowman, M Padgett, R Di Leonardo
We demonstrate that Digital Holographic Microscopy can be used for accurate 3D tracking and sizing of a colloidal probe trapped in a diamond anvil cell (DAC). Polystyrene beads were optically trapped in water up to Gigapascal pressures while simultaneously recording in-line holograms at 1 KHz frame rate. Using Lorenz-Mie scattering theory to fit interference patterns, we detected a 10% shrinking in the bead's radius due to the high applied pressure. Accurate bead sizing is crucial for obtaining reliable viscosity measurements and provides a convenient optical tool for the determination of the bulk modulus of probe material...
November 14, 2016: Optics Express
Dario Donnarumma, Alexey Brodoline, Daniel Alexandre, Michel Gross
An original technique that combines digital holography, dual illumination of the sample and cleaning algorithm 3D reconstruction is proposed. It uses a standard transmission microscopy setup coupled with a digital holography detection. The technique is 4D, since it allows to determine, at each time step, the 3D locations (x,y,z) of many moving objects that scatter the dual illumination beam. The technique has been validated by imaging the microcirculation of blood in a fish larvae sample (the moving objects are thus red blood cells RBCs)...
November 14, 2016: Optics Express
Anna Wang, Rees F Garmann, Vinothan N Manoharan
We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium's body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell's three-dimensional (3D) position and orientation and recover behavior such as body angle rotation during runs, tumbles, and pole reversal. Our technique is label-free and capable of frame rates limited only by the camera...
October 17, 2016: Optics Express
A V Belashov, A A Zhikhoreva, T N Belyaeva, E S Kornilova, N V Petrov, A V Salova, I V Semenova, O S Vasyutinskii
A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.
November 1, 2016: Optics Letters
Vimal Prabhu Pandiyan, Kedar Khare, Renu John
No abstract text is available yet for this article.
October 1, 2016: Journal of Biomedical Optics
Mehdi Molaei, Jian Sheng
Understanding how bacteria move close to a surface under various stimuli is crucial for a broad range of microbial processes including biofilm formation, bacterial transport and migration. While prior studies focus on interactions between single stimulus and bacterial suspension, we emphasize on compounding effects of flow shear and solid surfaces on bacterial motility, especially reorientation and tumble. We have applied microfluidics and digital holographic microscopy to capture a large number (>10(5)) of 3D Escherichia coli trajectories near a surface under various flow shear...
October 18, 2016: Scientific Reports
Anna Wang, Ryan McGorty, David M Kaz, Vinothan N Manoharan
Previous experiments have shown that spherical colloidal particles relax to equilibrium slowly after they adsorb to a liquid-liquid interface, despite the large interfacial energy gradient driving the adsorption. The slow relaxation has been explained in terms of transient pinning and depinning of the contact line on the surface of the particles. However, the nature of the pinning sites has not been investigated in detail. We use digital holographic microscopy to track a variety of colloidal spheres-inorganic and organic, charge-stabilized and sterically stabilized, aqueous and non-aqueous-as they breach liquid interfaces...
October 13, 2016: Soft Matter
Khaled M Yassien, Mostafa Agour
A method for studying the effect of temperature on the optical properties of basalt fiber is presented. It is based on recording a set of phase-shifted digital holograms for the sample under the test. The holograms are obtained utilizing a system based on Mach-Zehnder interferometer, where the fiber sample inserted in an immersion liquid is placed within a temperature controlled chamber. From the recorded digital holograms the optical path differences which are used to calculate the refractive indices are determined...
October 11, 2016: Microscopy Research and Technique
Chen Wang, Fook Chiong Cheong, David B Ruffner, Xiao Zhong, Michael D Ward, David G Grier
In-line holographic microscopy images of micrometer-scale fractal aggregates can be interpreted with an effective-sphere model to obtain each aggregate's size and the population-averaged fractal dimension. We demonstrate this technique experimentally using model fractal clusters of polystyrene nanoparticles and fractal protein aggregates composed of bovine serum albumin and bovine pancreas insulin.
October 26, 2016: Soft Matter
Alborz Feizi, Yibo Zhang, Alon Greenbaum, Alex Guziak, Michelle Luong, Raymond Yan Lok Chan, Brandon Berg, Haydar Ozkan, Wei Luo, Michael Wu, Yichen Wu, Aydogan Ozcan
Monitoring yeast cell viability and concentration is important in brewing, baking and biofuel production. However, existing methods of measuring viability and concentration are relatively bulky, tedious and expensive. Here we demonstrate a compact and cost-effective automatic yeast analysis platform (AYAP), which can rapidly measure cell concentration and viability. AYAP is based on digital in-line holography and on-chip microscopy and rapidly images a large field-of-view of 22.5 mm(2). This lens-free microscope weighs 70 g and utilizes a partially-coherent illumination source and an opto-electronic image sensor chip...
September 30, 2016: Lab on a Chip
Philipp Lenz, Markus Brückner, Steffi Ketelhut, Jan Heidemann, Björn Kemper, Dominik Bettenworth
The incidence of inflammatory bowel disease, i.e., Crohn's disease and Ulcerative colitis, has significantly increased over the last decade. The etiology of IBD remains unknown and current therapeutic strategies are based on the unspecific suppression of the immune system. The development of treatments that specifically target intestinal inflammation and epithelial wound healing could significantly improve management of IBD, however this requires accurate detection of inflammatory changes. Currently, potential drug candidates are usually evaluated using animal models in vivo or with cell culture based techniques in vitro...
2016: Journal of Visualized Experiments: JoVE
C Grassberger, P Dinkelborg, A McNamara, J P Schuemann, S J McMahon, H Willers, H Paganetti, M Wang
No abstract text is available yet for this article.
October 1, 2016: International Journal of Radiation Oncology, Biology, Physics
Jianglei Di, Ying Li, Min Xie, Jiwei Zhang, Chaojie Ma, Teli Xi, Enpu Li, Jianlin Zhao
A dual-wavelength common-path digital holographic microscopy based on a single parallel glass plate is presented to achieve quantitative phase imaging, which combines the dual-wavelength technique with lateral shearing interferometry. Two illumination laser beams with different wavelengths (λ<sub>1</sub>=532  nm and λ<sub>2</sub>=632.8  nm) are reflected by the front and back surfaces of the parallel glass plate to create the lateral shear and form the digital hologram, and then the hologram is reconstructed to obtain the phase distribution with a synthetic wavelength Λ=3339...
September 10, 2016: Applied Optics
Maciej Trusiak, Vicente Mico, Javier Garcia, Krzysztof Patorski
We propose a novel single-shot Hilbert-Huang transform-based algorithm applied to digital holographic microscopy (DHM) for robust, fast, and accurate single-shot quantitative phase imaging in on-axis and off-axis configurations. Fringe pattern with possible defects and closed fringes are adaptively filtered and accurately phase demodulated using local fringe direction estimation. Experimental validation of the proposed techniques is presented as the DHM study of microbeads and red blood cells phase samples...
September 15, 2016: Optics Letters
Jérôme Dohet-Eraly, Catherine Yourassowsky, Frank Dubois
The knowledge of the complex amplitude of optical fields, that is, both quantitative phase and intensity, enables numeric reconstruction along the optical axis. Nonetheless, a criterion is required for autofocusing. This Letter presents a robust and rapid refocusing criterion suitable for color interferometric digital holographic microscopy, and, more generally, for applications where complex amplitude is known for at least two different wavelengths. This criterion uses the phase in the Fourier domain, which is compared among wavelengths...
September 1, 2016: Optics Letters
Kyoohyun Kim, Kibaek Choe, Inwon Park, Pilhan Kim, YongKeun Park
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use of labelling and also provide only qualitative imaging information. Holographic imaging approach based on measuring the refractive index distributions of cells, however, circumvent these problems and offer quantitative and label-free imaging capability. Here, we demonstrate in vivo two- and three-dimensional holographic imaging of circulating blood cells in intact microcapillaries of live mice...
2016: Scientific Reports
Vitor Brasiliense, Pascal Berto, Catherine Combellas, Gilles Tessier, Frédéric Kanoufi
Interest in nanoparticles has vigorously increased over the last 20 years as more and more studies show how their use can potentially revolutionize science and technology. Their applications span many different academically and industrially relevant fields such as catalysis, materials science, health, etc. Until the past decade, however, nanoparticle studies mostly relied on ensemble studies, thus leaving aside their chemical heterogeneity at the single particle level. Over the past few years, powerful new tools appeared to probe nanoparticles individually and in situ...
September 20, 2016: Accounts of Chemical Research
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