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Holographic microscopy

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https://www.readbyqxmd.com/read/28081471/in-situ-morphological-assessment-of-apoptosis-induced-by-phaleria-macrocarpa-boerl-fruit-ethyl-acetate-fraction-pmeaf-in-mda-mb-231-cells-by-microscopy-observation
#1
Nowroji Kavitha, Yeng Chen, Jagat R Kanwar, Sreenivasan Sasidharan
Phaleria macrocarpa (Boerl.) is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases. The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. The cytotoxicity of PMEAF treated MDA-MB-231 cells was determined through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay and CyQuant Cell Proliferation Assay after 24h of treatment...
January 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28081110/light-sheet-microscopy-in-a-glass-capillary-feedback-holographic-control-for-illumination-beam-correction
#2
Tobias Meinert, Benjamin Alexander Gutwein, Alexander Rohrbach
Light-sheet microscopy enables fast 3D, high-contrast imaging in biology and colloidal sciences. Recently, the controlled transport of living embryos or small colloids through stable glass capillaries is manifold interesting. Although they hardly impair the sample, glass capillaries spoil the image by generating significant aberrations of the illumination and detection light. Here, we analyze the deflection of illuminating Bessel beams at the capillary by k-spectral shifting, and correct for it by a beam deflector...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28081109/non-axial-scanning-multifocal-confocal-microscopy-with-multiplexed-volume-holographic-gratings
#3
Po-Hao Wang, Vijay Raj Singh, Jau-Min Wong, Kung-Bin Sung, Yuan Luo
Confocal imaging techniques offer an optical sectioning capability to acquire three-dimensional information from various volumetric samples by discriminating the desired in-focus signals from the out-of-focus background. However, confocal, in general, requires a point-by-point scan in both the lateral and axial directions to reconstruct three-dimensional images. In addition, axial scanning in confocal is slower than scanning in lateral directions. In this Letter, a non-axial-scanning multifocal confocal microscope incorporating multiplexed holographic gratings in illumination and dual detection for depth discrimination is presented...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28081079/simple-and-fast-spectral-domain-algorithm-for-quantitative-phase-imaging-of-living-cells-with-digital-holographic-microscopy
#4
Junwei Min, Baoli Yao, Steffi Ketelhut, Christian Engwer, Burkhard Greve, Björn Kemper
We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28059271/lossless-and-lossy-compression-of-quantitative-phase-images-of-red-blood-cells-obtained-by-digital-holographic-imaging
#5
Keyvan Jaferzadeh, Samaneh Gholami, Inkyu Moon
In this paper, we evaluate lossless and lossy compression techniques to compress quantitative phase images of red blood cells (RBCs) obtained by an off-axis digital holographic microscopy (DHM). The RBC phase images are numerically reconstructed from their digital holograms and are stored in 16-bit unsigned integer format. In the case of lossless compression, predictive coding of JPEG lossless (JPEG-LS), JPEG2000, and JP3D are evaluated, and compression ratio (CR) and complexity (compression time) are compared against each other...
December 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/28059249/automatic-full-compensation-of-quantitative-phase-imaging-in-off-axis-digital-holographic-microscopy
#6
Carlos Trujillo, Raúl Castañeda, Pablo Piedrahita-Quintero, Jorge Garcia-Sucerquia
An automatic method that fully compensates the quantitative phase measurements in off-axis digital holographic microscopy (DHM) is presented. The two main perturbations of the quantitative phase measurements in off-axis DHM are automatically removed. While the curvature phase flaw introduced by the microscope objective is avoided by the use of an optimized telecentric imaging system for the recording of the holograms, the remaining phase perturbation due to the tilt of the reference wave is removed by the automatic computation of a digital compensating reference wave...
December 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/28059181/simultaneous-two-wavelength-phase-unwrapping-using-an-external-module-for-multiplexing-off-axis-holography
#7
Nir A Turko, Natan T Shaked
We present a dual-wavelength external holographic microscopy module for quantitative phase imaging of 3D structures with extended thickness range. This is done by simultaneous acquisition of two off-axis interferograms, each at a different wavelength, and generation of a synthetic wavelength, which is larger than the sample optical thickness, allowing two-wavelength unwrapping. The simultaneous acquisition is carried out by using optical multiplexing of the two interferograms onto the camera, where each of them has orthogonal off-axis interference fringe direction in relation to the other one...
January 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28006045/digital-holographic-microscopy-of-phase-separation-in-multicomponent-lipid-membranes
#8
Vahideh Farzam Rad, Ali-Reza Moradi, Ahmad Darudi, Lobat Tayebi
No abstract text is available yet for this article.
December 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27958590/single-shot-digital-holographic-microscopy-for-quantifying-a-spatially-resolved-jones-matrix-of-biological-specimens
#9
Taeseok Daniel Yang, Kwanjun Park, Yong Guk Kang, Kyoung J Lee, Beop-Min Kim, Youngwoon Choi
Field-based polarization measurements are essential for the completeness of information when exploiting the complex nature of optical responses of target objects. Here, we demonstrate digital holographic microscopy for quantifying a polarization-sensitive map of an object with a single-shot measurement. Using the image-splitting device generating four different copies of an object image and a separate reference beam of an off-axis configuration enables single-shot and multi-imaging capability. With the use of two polarization filters, four complex field images containing an object's polarization response are obtained simultaneously...
December 12, 2016: Optics Express
https://www.readbyqxmd.com/read/27958271/nanoscopy-of-bacterial-cells-immobilized-by-holographic-optical-tweezers
#10
Robin Diekmann, Deanna L Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolution well below the diffraction limit using localization microscopy...
December 13, 2016: Nature Communications
https://www.readbyqxmd.com/read/27906347/versatile-spectral-modulation-of-a-broadband-source-for-digital-holographic-microscopy
#11
Gaël Nardin, Tristan Colomb, Yves Emery, Christophe Moser
We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a 'pulse-shaping' geometry, the spatial light modulator provides a versatile control over the bandwidth and wavelength of the light source. The control of these properties enables adaptation to various experimental conditions. As a first application, we show that the source bandwidth can be adapted to the off-axis geometry to provide quantitative phase imaging over the whole field of view...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27901048/sparsity-based-multi-height-phase-recovery-in-holographic-microscopy
#12
Yair Rivenson, Yichen Wu, Hongda Wang, Yibo Zhang, Alborz Feizi, Aydogan Ozcan
High-resolution imaging of densely connected samples such as pathology slides using digital in-line holographic microscopy requires the acquisition of several holograms, e.g., at >6-8 different sample-to-sensor distances, to achieve robust phase recovery and coherent imaging of specimen. Reducing the number of these holographic measurements would normally result in reconstruction artifacts and loss of image quality, which would be detrimental especially for biomedical and diagnostics-related applications...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27869846/simultaneous-measurement-of-refractive-index-distribution-and-topography-by-integrated-transmission-and-reflection-digital-holographic-microscopy
#13
Chaojie Ma, Jianglei Di, Jiwei Zhang, Ying Li, Teli Xi, Enpu Li, Jianlin Zhao
We propose a method for simultaneously measuring dynamic changes of the refractive index distribution and surface topography, which integrates the transmission and reflection digital holographic microscopy based on polarization and angular multiplexing techniques. The complex amplitudes of the transmitted and reflected object waves can be simultaneously retrieved. The phase information of the reflected object wave is directly used to determine the topography of the specimen which corresponds to its physical thickness...
November 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27857428/holographic-tracking-and-sizing-of-optically-trapped-microprobes-in-diamond-anvil-cells
#14
F Saglimbeni, S Bianchi, G Gibson, R Bowman, M Padgett, R Di Leonardo
We demonstrate that Digital Holographic Microscopy can be used for accurate 3D tracking and sizing of a colloidal probe trapped in a diamond anvil cell (DAC). Polystyrene beads were optically trapped in water up to Gigapascal pressures while simultaneously recording in-line holograms at 1 KHz frame rate. Using Lorenz-Mie scattering theory to fit interference patterns, we detected a 10% shrinking in the bead's radius due to the high applied pressure. Accurate bead sizing is crucial for obtaining reliable viscosity measurements and provides a convenient optical tool for the determination of the bulk modulus of probe material...
November 14, 2016: Optics Express
https://www.readbyqxmd.com/read/27857417/4d-holographic-microscopy-of-zebrafish-larvae-microcirculation
#15
Dario Donnarumma, Alexey Brodoline, Daniel Alexandre, Michel Gross
An original technique that combines digital holography, dual illumination of the sample and cleaning algorithm 3D reconstruction is proposed. It uses a standard transmission microscopy setup coupled with a digital holography detection. The technique is 4D, since it allows to determine, at each time step, the 3D locations (x,y,z) of many moving objects that scatter the dual illumination beam. The technique has been validated by imaging the microcirculation of blood in a fish larvae sample (the moving objects are thus red blood cells RBCs)...
November 14, 2016: Optics Express
https://www.readbyqxmd.com/read/27828208/tracking-e-coli-runs-and-tumbles-with-scattering-solutions-and-digital-holographic-microscopy
#16
Anna Wang, Rees F Garmann, Vinothan N Manoharan
We use in-line digital holographic microscopy to image freely swimming E. coli. We show that fitting a light scattering model to E. coli holograms can yield quantitative information about the bacterium's body rotation and tumbles, offering a precise way to track fine details of bacterial motility. We are able to extract the cell's three-dimensional (3D) position and orientation and recover behavior such as body angle rotation during runs, tumbles, and pole reversal. Our technique is label-free and capable of frame rates limited only by the camera...
October 17, 2016: Optics Express
https://www.readbyqxmd.com/read/27805679/digital-holographic-microscopy-in-label-free-analysis-of-cultured-cells-response-to-photodynamic-treatment
#17
A V Belashov, A A Zhikhoreva, T N Belyaeva, E S Kornilova, N V Petrov, A V Salova, I V Semenova, O S Vasyutinskii
A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.
November 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27768784/quantitative-phase-imaging-of-live-cells-with-near-on-axis-digital-holographic-microscopy-using-constrained-optimization-approach
#18
Vimal Prabhu Pandiyan, Kedar Khare, Renu John
No abstract text is available yet for this article.
October 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27752062/succeed-escape-flow-shear-promotes-tumbling-of-escherichia-colinear-a-solid-surface
#19
Mehdi Molaei, Jian Sheng
Understanding how bacteria move close to a surface under various stimuli is crucial for a broad range of microbial processes including biofilm formation, bacterial transport and migration. While prior studies focus on interactions between single stimulus and bacterial suspension, we emphasize on compounding effects of flow shear and solid surfaces on bacterial motility, especially reorientation and tumble. We have applied microfluidics and digital holographic microscopy to capture a large number (>10(5)) of 3D Escherichia coli trajectories near a surface under various flow shear...
October 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27734049/contact-line-pinning-controls-how-quickly-colloidal-particles-equilibrate-with-liquid-interfaces
#20
Anna Wang, Ryan McGorty, David M Kaz, Vinothan N Manoharan
Previous experiments have shown that spherical colloidal particles relax to equilibrium slowly after they adsorb to a liquid-liquid interface, despite the large interfacial energy gradient driving the adsorption. The slow relaxation has been explained in terms of transient pinning and depinning of the contact line on the surface of the particles. However, the nature of the pinning sites has not been investigated in detail. We use digital holographic microscopy to track a variety of colloidal spheres-inorganic and organic, charge-stabilized and sterically stabilized, aqueous and non-aqueous-as they breach liquid interfaces...
October 13, 2016: Soft Matter
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