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Holographic microscopy

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https://www.readbyqxmd.com/read/28298087/landing-dynamics-of-swimming-bacteria-on-a-polymeric-surface-effect-of-surface-properties
#1
Meng Qi, Xiangjun Gong, Bo Wu, Guangzhao Zhang
Landing of bacteria for adhesion on a surface is a common phenomenon in our life. However, how surface properties involve in this process remains largely unclear. Using digital holographic microscopy (DHM), we have investigated the three-dimensional (3D) motions of flagellate E. coli swimming near polymeric surfaces with different properties in aqueous solution before adhesion. We monitor the bacteria landing dynamics, which shows that the density distribution, the probability and orientation for collisions of the bacteria are determined by their motility but slightly affected by the surface properties...
March 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28270966/label-free-fingerprinting-of-tumor-cells-in-bulk-flow-using-inline-digital-holographic-microscopy
#2
Dhananjay Kumar Singh, Caroline C Ahrens, Wei Li, Siva A Vanapalli
Large-scale and label-free phenotyping of cells holds great promise in medicine, especially in cancer diagnostics and prognosis. Here, we introduce inline digital holography microscopy for volumetric imaging of cells in bulk flow and fingerprinting of flowing tumor cells based on two metrics, in-focus scattered intensity and cell diameter. Using planar distribution of immobilized particles, we identify the optimal recording distance and microscope objective magnification that minimizes the error in measurement of particle position, size and scattered intensity...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28264140/quantitative-phase-imaging-for-cell-culture-quality-control
#3
Lena Kastl, Michael Isbach, Dieter Dirksen, Jürgen Schnekenburger, Björn Kemper
The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium...
March 6, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28256551/multimodal-discrimination-of-immune-cells-using-a-combination-of-raman-spectroscopy-and-digital-holographic-microscopy
#4
Naomi McReynolds, Fiona G M Cooke, Mingzhou Chen, Simon J Powis, Kishan Dholakia
The ability to identify and characterise individual cells of the immune system under label-free conditions would be a significant advantage in biomedical and clinical studies where untouched and unmodified cells are required. We present a multi-modal system capable of simultaneously acquiring both single point Raman spectra and digital holographic images of single cells. We use this combined approach to identify and discriminate between immune cell populations CD4+ T cells, B cells and monocytes. We investigate several approaches to interpret the phase images including signal intensity histograms and texture analysis...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28248333/superresolved-spatially-multiplexed-interferometric-microscopy
#5
José Ángel Picazo-Bueno, Zeev Zalevsky, Javier García, Vicente Micó
Superresolution capability by angular and time multiplexing is implemented onto a regular microscope. The technique, named superresolved spatially multiplexed interferometric microscopy (S2MIM), follows our previously reported SMIM technique [Opt. Express22, 14929 (2014)OPEXFF1094-408710.1364/OE.22.014929, J. Biomed. Opt.21, 106007 (2016)JBOPFO1083-366810.1117/1.JBO.21.10.106007] improved with superresolved imaging. All together, S2MIM updates a commercially available non-holographic microscope into a superresolved holographic one...
March 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28240818/imaging-of-dense-cell-cultures-by-multiwavelength-lens-free-video-microscopy
#6
C Allier, S Morel, R Vincent, L Ghenim, F Navarro, M Menneteau, T Bordy, L Hervé, O Cioni, X Gidrol, Y Usson, J-M Dinten
They present results for lens-free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm(2) ) over a large field of view of 29.4 mm(2) . Combined with the compactness of the system which fits altogether inside an incubator, lens-free microscopy becomes a unique tool to monitor cell cultures over several days...
February 27, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28155267/flexible-heteroepitaxy-of-cofe2o4-muscovite-bimorph-with-large-magnetostriction
#7
Heng-Jui Liu, Chih-Kuo Wang, Dong Su, Tahta Amrillah, Ying-Hui Hsieh, Kun-Hong Wu, Yi-Chun Chen, Jenh-Yih Juang, Lukas M Eng, Shien-Uang Jen, Ying-Hao Chu
A bimorph composed of ferrimagnetic cobalt ferrite (CoFe2O4, CFO) and flexible muscovite was fabricated via van der Waals epitaxy. The combination of X-ray diffraction and transmission electron microscopy was conducted to reveal the heteroepitaxy of the CFO/muscovite system. The robust magnetic behaviors against mechanical bending were characterized by hysteresis measurements and magnetic force microscopy, which maintain a saturation magnetization (Ms) of ∼120-150 emu/cm(3) under different bending states...
March 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28117428/focusing-and-alignment-of-erythrocytes-in-a-viscoelastic-medium
#8
Taesik Go, Hyeokjun Byeon, Sang Joon Lee
Viscoelastic fluid flow-induced cross-streamline migration has recently received considerable attention because this process provides simple focusing and alignment over a wide range of flow rates. The lateral migration of particles depends on the channel geometry and physicochemical properties of particles. In this study, digital in-line holographic microscopy (DIHM) is employed to investigate the lateral migration of human erythrocytes induced by viscoelastic fluid flow in a rectangular microchannel. DIHM provides 3D spatial distributions of particles and information on particle orientation in the microchannel...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28081471/in-situ-morphological-assessment-of-apoptosis-induced-by-phaleria-macrocarpa-boerl-fruit-ethyl-acetate-fraction-pmeaf-in-mda-mb-231-cells-by-microscopy-observation
#9
Nowroji Kavitha, Yeng Chen, Jagat R Kanwar, Sreenivasan Sasidharan
Phaleria macrocarpa (Boerl.) is a well-known medicinal plant and have been extensively used as traditional medicine for ages in treatment of various diseases. The purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. The cytotoxicity of PMEAF treated MDA-MB-231 cells was determined through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay and CyQuant Cell Proliferation Assay after 24h of treatment...
March 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28081110/light-sheet-microscopy-in-a-glass-capillary-feedback-holographic-control-for-illumination-beam-correction
#10
Tobias Meinert, Benjamin Alexander Gutwein, Alexander Rohrbach
Light-sheet microscopy enables fast 3D, high-contrast imaging in biology and colloidal sciences. Recently, the controlled transport of living embryos or small colloids through stable glass capillaries is manifold interesting. Although they hardly impair the sample, glass capillaries spoil the image by generating significant aberrations of the illumination and detection light. Here, we analyze the deflection of illuminating Bessel beams at the capillary by k-spectral shifting, and correct for it by a beam deflector...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28081109/non-axial-scanning-multifocal-confocal-microscopy-with-multiplexed-volume-holographic-gratings
#11
Po-Hao Wang, Vijay Raj Singh, Jau-Min Wong, Kung-Bin Sung, Yuan Luo
Confocal imaging techniques offer an optical sectioning capability to acquire three-dimensional information from various volumetric samples by discriminating the desired in-focus signals from the out-of-focus background. However, confocal, in general, requires a point-by-point scan in both the lateral and axial directions to reconstruct three-dimensional images. In addition, axial scanning in confocal is slower than scanning in lateral directions. In this Letter, a non-axial-scanning multifocal confocal microscope incorporating multiplexed holographic gratings in illumination and dual detection for depth discrimination is presented...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28081079/simple-and-fast-spectral-domain-algorithm-for-quantitative-phase-imaging-of-living-cells-with-digital-holographic-microscopy
#12
Junwei Min, Baoli Yao, Steffi Ketelhut, Christian Engwer, Burkhard Greve, Björn Kemper
We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method...
January 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28059271/lossless-and-lossy-compression-of-quantitative-phase-images-of-red-blood-cells-obtained-by-digital-holographic-imaging
#13
Keyvan Jaferzadeh, Samaneh Gholami, Inkyu Moon
In this paper, we evaluate lossless and lossy compression techniques to compress quantitative phase images of red blood cells (RBCs) obtained by an off-axis digital holographic microscopy (DHM). The RBC phase images are numerically reconstructed from their digital holograms and are stored in 16-bit unsigned integer format. In the case of lossless compression, predictive coding of JPEG lossless (JPEG-LS), JPEG2000, and JP3D are evaluated, and compression ratio (CR) and complexity (compression time) are compared against each other...
December 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/28059249/automatic-full-compensation-of-quantitative-phase-imaging-in-off-axis-digital-holographic-microscopy
#14
Carlos Trujillo, Raúl Castañeda, Pablo Piedrahita-Quintero, Jorge Garcia-Sucerquia
An automatic method that fully compensates the quantitative phase measurements in off-axis digital holographic microscopy (DHM) is presented. The two main perturbations of the quantitative phase measurements in off-axis DHM are automatically removed. While the curvature phase flaw introduced by the microscope objective is avoided by the use of an optimized telecentric imaging system for the recording of the holograms, the remaining phase perturbation due to the tilt of the reference wave is removed by the automatic computation of a digital compensating reference wave...
December 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/28059181/simultaneous-two-wavelength-phase-unwrapping-using-an-external-module-for-multiplexing-off-axis-holography
#15
Nir A Turko, Natan T Shaked
We present a dual-wavelength external holographic microscopy module for quantitative phase imaging of 3D structures with extended thickness range. This is done by simultaneous acquisition of two off-axis interferograms, each at a different wavelength, and generation of a synthetic wavelength, which is larger than the sample optical thickness, allowing two-wavelength unwrapping. The simultaneous acquisition is carried out by using optical multiplexing of the two interferograms onto the camera, where each of them has orthogonal off-axis interference fringe direction in relation to the other one...
January 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28006045/digital-holographic-microscopy-of-phase-separation-in-multicomponent-lipid-membranes
#16
Vahideh Farzam Rad, Ali-Reza Moradi, Ahmad Darudi, Lobat Tayebi
No abstract text is available yet for this article.
December 1, 2016: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/27958590/single-shot-digital-holographic-microscopy-for-quantifying-a-spatially-resolved-jones-matrix-of-biological-specimens
#17
Taeseok Daniel Yang, Kwanjun Park, Yong Guk Kang, Kyoung J Lee, Beop-Min Kim, Youngwoon Choi
Field-based polarization measurements are essential for the completeness of information when exploiting the complex nature of optical responses of target objects. Here, we demonstrate digital holographic microscopy for quantifying a polarization-sensitive map of an object with a single-shot measurement. Using the image-splitting device generating four different copies of an object image and a separate reference beam of an off-axis configuration enables single-shot and multi-imaging capability. With the use of two polarization filters, four complex field images containing an object's polarization response are obtained simultaneously...
December 12, 2016: Optics Express
https://www.readbyqxmd.com/read/27958271/nanoscopy-of-bacterial-cells-immobilized-by-holographic-optical-tweezers
#18
Robin Diekmann, Deanna L Wolfson, Christoph Spahn, Mike Heilemann, Mark Schüttpelz, Thomas Huser
Imaging non-adherent cells by super-resolution far-field fluorescence microscopy is currently not possible because of their rapid movement while in suspension. Holographic optical tweezers (HOTs) enable the ability to freely control the number and position of optical traps, thus facilitating the unrestricted manipulation of cells in a volume around the focal plane. Here we show that immobilizing non-adherent cells by optical tweezers is sufficient to achieve optical resolution well below the diffraction limit using localization microscopy...
December 13, 2016: Nature Communications
https://www.readbyqxmd.com/read/27906347/versatile-spectral-modulation-of-a-broadband-source-for-digital-holographic-microscopy
#19
Gaël Nardin, Tristan Colomb, Yves Emery, Christophe Moser
We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a 'pulse-shaping' geometry, the spatial light modulator provides a versatile control over the bandwidth and wavelength of the light source. The control of these properties enables adaptation to various experimental conditions. As a first application, we show that the source bandwidth can be adapted to the off-axis geometry to provide quantitative phase imaging over the whole field of view...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27901048/sparsity-based-multi-height-phase-recovery-in-holographic-microscopy
#20
Yair Rivenson, Yichen Wu, Hongda Wang, Yibo Zhang, Alborz Feizi, Aydogan Ozcan
High-resolution imaging of densely connected samples such as pathology slides using digital in-line holographic microscopy requires the acquisition of several holograms, e.g., at >6-8 different sample-to-sensor distances, to achieve robust phase recovery and coherent imaging of specimen. Reducing the number of these holographic measurements would normally result in reconstruction artifacts and loss of image quality, which would be detrimental especially for biomedical and diagnostics-related applications...
November 30, 2016: Scientific Reports
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