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endometrial stem cell

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https://www.readbyqxmd.com/read/29340051/microrna-34a-inhibits-cells-proliferation-and-invasion-by-downregulating-notch1-in-endometrial-cancer
#1
Zhen Wang, Wei Wang, Kangrong Huang, Yueling Wang, Jing Li, Xinyuan Yang
MicroRNAs (miRNAs) are small non-coding RNAs composed of 18-25 nucleotides that regulate the expression of approximately 30% of human protein coding genes. Dysregulation of miRNAs plays a pivotal role in the initiation and progression of malignancies. Our study has shown that microRNA-34a (miR-34a) was upregulated in human endometrial cancer stem cells (ECSCs). However, it is unknown how miR-34a regulates endometrial cancer itself. Here, we report that miR-34a directly and functionally targeted Notch1. MiR-34a inhibited the proliferation, migration, invasion, EMT-associated phenotypes by downregulating Notch1 in endometrial cancer cells...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29329377/endometrial-stem-cell-derived-g-csf-attenuates-endometrial-fibrosis-via-sonic-hedgehog-transcriptional-activator-gli2
#2
Xiaona Lin, Yanling Zhang, Yibin Pan, Shilin He, Yongdong Dai, Bingqing Zhu, Cheng Wei, Liaobing Xin, Wenzhi Xu, Chunsheng Xiang, Songying Zhang
Intrauterine adhesion (IUA) is characterized by endometrial fibrosis, which ultimately leads to menstrual abnormalities, infertility and recurrent miscarriages. The Shh/Gli2 pathway plays a critical role in tissue fibrogenesis and regeneration; Gli2 activation induces profibrogenic effects in various tissues, such as the liver and kidney. However, the role of Gli2 in endometrial fibrosis remains unknown. The purpose of this study was to test the hypothesis that activated Gli2 promotes endometrial fibrosis. Endometrial samples from moderate and severe IUA patients exhibited significantly enhanced expression of Gli2 compared with normal endometrial samples and mild IUA samples...
January 10, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29278305/biological-characteristics-of-human-menstrual-blood-derived-endometrial-stem-cells
#3
Yanli Liu, Rongcheng Niu, Fen Yang, Yan Yan, Shengying Liang, Yuliang Sun, Ping Shen, Juntang Lin
Successful isolation of human endometrial stem cells from menstrual blood, namely menstrual blood-derived endometrial stem cells (MenSCs), has provided enticing alternative seed cells for stem cell-based therapy. MenSCs are enriched in the self-regenerative tissue, endometrium, which shed along the periodic menstrual blood and thus their acquisition involves no physical invasiveness. However, the impact of the storage duration of menstrual blood prior to stem cell isolation, the age of the donor, the number of passages on the self-renewing of MenSCs, the paracrine production of biological factors in MenSCs and expression of adhesion molecules on MenSCs remain elusive...
December 26, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29275599/-hypoxia-inducible-factor-1%C3%AE-desumoylation-reduces-the-stemness-maintenance-ability-of-endometrial-cancer-stem-cell-and-increases-its-chemosensitivity
#4
L Yuan, Z M Jiang, X H Chen, X Y Bian, Y X Li, X F Ma, X Z Liu
Objective: To reduce the stemness maintenance ability of endometrial cancer stem cell and increase its sensitivity to chemotherapy by inducing hypoxia inducible factor 1α (HIF-1α) protein deSUMOylation. Methods: Lentiviral plasmid mediated ubiquitin carrier protein 9 (Ubc9) gene silencing was transgened into KLE endometrial carcinoma cells. The expression of Ubc9, small ubiquitin-related modifier 1(SUMO1) and HIF-1α protein was detected by Western blotting. Then tumor stem cells clones were cultured in 96 well plates, and these clone balls diameter were calculated...
December 5, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29241917/pueraria-mirifica-inhibits-17%C3%AE-estradiol-induced-cell-proliferation-of%C3%A2-human-endometrial-mesenchymal-stem-cells
#5
Ta-Chin Lin, Kai-Hung Wang, An-Pei Kao, Kuo-Hsiang Chuang, Tsung-Cheng Kuo
OBJECTIVE: The notion that the human endometrium may contain a population of stem cells has recently been proposed. The mesenchymal stem cells (MSCs) in the endometrium are believed to be responsible for the remarkable regenerative ability of endometrial cells. Estrogens influence the physiological and pathological processes of several hormone-dependent tissues, such as the endometrium. Pueraria mirifica (PM) is a herbal plant that contains several phytoestrogens, including isoflavones, lignans, and coumestans, and is known to exert an estrogenic effect on animal models...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29227245/clearance-of-senescent-decidual-cells-by-uterine-natural-killer-cells-in-cycling-human-endometrium
#6
Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara, Joanne Muter, Emma S Lucas, Taihei Yamada, Laura Woods, Raffaella Lucciola, Yie Hou Lee, Satoru Takeda, Sascha Ott, Myriam Hemberger, Siobhan Quenby, Jan Joris Brosens
In cycling human endometrium, menstruation is followed by rapid estrogen-dependent growth. Upon ovulation, progesterone and rising cellular cAMP levels activate the transcription factor Forkhead box O1 (FOXO1) in endometrial stromal cells (EnSCs), leading to cell cycle exit and differentiation into decidual cells that control embryo implantation. Here we show that FOXO1 also causes acute senescence of a subpopulation of decidualizing EnSCs in an IL-8 dependent manner. Selective depletion or enrichment of this subpopulation revealed that decidual senescence drives the transient inflammatory response associated with endometrial receptivity...
December 11, 2017: ELife
https://www.readbyqxmd.com/read/29215321/mesenchymal-endometrial-stem-stromal-cells-for-hard-tissue-engineering-a-review-of-in-vitro-and-in-vivo-evidence
#7
Fahimeh S Tabatabaei, Jafar Ai
Hard tissues including teeth, bone and cartilage have inability or poor capacity to self-renew, especially in large defects. Therefore, repair of damages in these tissues represents a huge challenge in the medical field today. Hard tissue engineering commonly utilizes different stem cell sources as a promising strategy for treating bone, cartilages and tooth defects or disorders. Decades ago, researchers successfully isolated and identified endometrial mesenchymal stem/stromal cells (EnSCs) and discovered their multidifferentiation potential...
December 7, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/29213289/endometritis-and-in-vitro-pge2-challenge-modify-properties-of-cattle-endometrial-mesenchymal-stem-cells-and-their-transcriptomic-profile
#8
Evelyn Lara, Alejandra Velásquez, Joel Cabezas, Nathaly Rivera, Paulina Pacha, Lleretny Rodríguez-Alvarez, Fernando Saravia, Fidel Ovidio Castro
Mesenchymal stem cells (MSCs) were isolated and characterized from postpartum bovine endometrium of animals with subclinical (n = 5) and clinical endometritis (n = 3) and healthy puerperal females (n = 5). Cells isolated displayed mean morphological features of MSCs and underwent osteogenic, chondrogenic, and adipogenic differentiation after induction (healthy and subclinical). Cells from cows with clinical endometritis did not undergo adipogenic differentiation. All cells expressed mRNAs for selected MSC markers...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29190956/peroxiredoxin-3-maintains-the-survival-of-endometrial-cancer-stem-cells-by-regulating-oxidative-stress
#9
In-Sung Song, Yu Jeong Jeong, Young Jin Seo, Jung Mi Byun, Young Nanm Kim, Dae Hoon Jeong, Jin Han, Ki Tae Kim, Sung-Wuk Jang
Cancer stem cell (CSC)-targeted therapy could reduce tumor growth, recurrence, and metastasis in endometrial cancer (EC). The mitochondria of CSCs have been recently found to be an important target for cancer treatment, but the mitochondrial features of CSCs and their regulators, which maintain mitochondrial function, remain unclear. Here, we investigated the mitochondrial properties of CSCs, and identified specific targets for eliminating CSCs in EC. We found that endometrial CSCs displayed higher mitochondrial membrane potential, Ca2+, reactive oxygen species, ATP levels, and oxygen consumption rates than non-CSCs...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29164582/mir-15a-5p-suppresses-endometrial-cancer-cell-growth-via-wnt-%C3%AE-catenin-signaling-pathway-by-inhibiting-wnt3a
#10
Z-M Wang, X-H Wan, G-Y Sang, J-D Zhao, Q-Y Zhu, D-M Wang
OBJECTIVE: Endometrial cancer is one of the three most common types of gynecologic cancer. The global incidence has increased in recent years. microRNAs (miRNAs) regulate numerous biological processes by binding to the 3'UTR of target mRNA to down-regulate protein synthesis. PATIENTS AND METHODS: Endometrial cancer patients received surgeries in our hospital were enrolled. MiR-15a-5p mimic or miR-15a-5p inhibitor was transfected into HEC-1-A cells by lentivirus...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29134714/identification-of-perivascular-and-stromal-mesenchymal-stem-progenitor-cells-in-porcine-endometrium
#11
J Wiater, M Niedziela, A Posmysz, K Wartalski, B Gajda, Z Smorąg, Z Rajfur, J Karasiński
Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29117721/enhanced-sciatic-nerve-regeneration-by-human-endometrial-stem-cells-in-an-electrospun-poly-%C3%AE%C2%B5-caprolactone-collagen-nbg-nerve-conduit-in-rat
#12
Forouzan Mohamadi, Somayeh Ebrahimi-Barough, Mohammad Reza Nourani, Korosh Mansoori, Majid Salehi, Ali Akbar Alizadeh, Seyed Mohammad Tavangar, Farshid Sefat, Siavash Sharifi, Jafar Ai
In recent years, for neurodegenerative diseases therapy, research has focused on the stem cells therapy. Due to promising findings in stem cell therapy, there are various sources of stem cells for transplantation in human. The aim of this study was to evaluate sciatic nerve regeneration in the rat after nerve transaction followed by human endometrial stem cells (hEnSCs) treatment into poly (e-caprolactone)/collagen/nanobioglass (PCL/collagen/NBG) nanofibrous conduits. After treatment of animals, the performance in motor and sensory tests, showed significant improvement in rats treated with hEnSCs as an autograft...
November 8, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29106744/germ-cell-tumor-growth-patterns-originating-from-clear-cell-carcinomas-of-the-ovary-and-endometrium-a-comparative-immunohistochemical-study-favouring-their-origin-from-somatic-stem-cells
#13
F F Nogales, J Prat, M Schuldt, N Cruz-Viruel, B Kau, E D'Angelo, X Matias-Guiu, A Vidal, W G McCluggage, J W Oosterhuis
AIMS: To report a series of 11 ovarian and one endometrial neoplasm in elderly patients with mixed clear cell tumour and germ cell tumour (GCT) components, to compare their immunohistochemical and demonstrate a putative stem cell population. METHODS AND RESULTS: The clear cell tumours included 11 clear cell carcinomas (CCC) and 1 borderline clear cell tumour, while the GCT always included glandular yolk sac tumour (YST). In 4 cases, there were also foci of teratoma with immature neuroepithelial and endodermal tissues, and undifferentiated areas showing true embryoids...
November 6, 2017: Histopathology
https://www.readbyqxmd.com/read/29105567/in-vivo-survival-of-human-endometrial-mesenchymal-stem-cells-transplanted-under-the-kidney-capsule-of-immunocompromised-mice
#14
Shanti Gurung, James A Deane, Saeedeh Darzi, Jerome A Werkmeister, Caroline E Gargett
Human endometrial mesenchymal stem cells (eMSCs) are a well-characterised adult stem cell type with potential for use in regenerative medicine or cell-therapy. As a proof of principle, we demonstrated that eMSCs promoted wound healing by reducing the inflammatory response through a paracrine action in a subcutaneous rat model of wound repair. However, an efficient protocol for culturing eMSCs in the undifferentiated state and a reliable method of labelling them for cell tracking were lacking. Here, we investigated the use of a lentiviral vector containing the mCherry fluorescent reporter gene to transduce and label eMSCs following in vitro culturing in A83-01-containing medium, and different methods of tracing the labelled cells following transplantation under the kidney capsule of immunocompromised NSG mice...
November 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29100623/pathogenesis-of-deep-endometriosis
#15
REVIEW
Stephan Gordts, Philippe Koninckx, Ivo Brosens
The pathophysiology of (deep) endometriosis is still unclear. As originally suggested by Cullen, change the definition "deeper than 5 mm" to "adenomyosis externa." With the discovery of the old European literature on uterine bleeding in 5%-10% of the neonates and histologic evidence that the bleeding represents decidual shedding, it is postulated/hypothesized that endometrial stem/progenitor cells, implanted in the pelvic cavity after birth, may be at the origin of adolescent and even the occasionally premenarcheal pelvic endometriosis...
October 31, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/29096715/differentiation-of-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-into-endometrial-cells
#16
Qin Shi, JingWei Gao, Yao Jiang, Baolan Sun, Wei Lu, Min Su, Yunzhao Xu, Xiaoqing Yang, Yuquan Zhang
BACKGROUND: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are a novel and promising strategy for tissue engineering because of their ability to differentiate into many cell types. We characterized the differentiation of WJ-MSCs into endometrial epithelial cell (EEC)-like and endometrial stromal cell (ESC)-like cells and assessed the effect of 17β-estradiol and 8-Br-cAMP on the differentiation system. METHODS: WJ-MSCs were treated in two ways to differentiate into EEC-like and ESC-like cells respectively: cocultured with ESCs in control/differentiation medium (17β-estradiol, growth factors); and cultured in control/differentiation medium (8-Br-cAMP alone or 8-Br-cAMP plus 17β-estrogen and growth factors)...
November 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29096246/isolation-of-stem-like-cancer-cells-in-primary-endometrial-cancer-using-cell-surface-markers-cd133-and-cxcr4
#17
Yi Sun, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Fang Wang, Chika Soko, Sigeru Saito, Toshio Nikaido
Endometrial cancer (EC) is the most common familiar gynecologic malignant tumor identified in the female reproductive system and has been increasing yearly. In this study, we will identify the surface markers and stem cell markers related with cancer stem cells (CSCs) of EC. Tissue samples were obtained from endometrial cancer patients during surgical procedures. Single cells were isolated from the tissues for culturing, transfection into nude mice, and histopathology analysis. RT-PCR demonstrated that the cultured cells strongly expressed stemness-related genes, such as c-Myc, Sox-2, Nanog, Oct 4A, ABCG2, BMI-1, CK-18, Nestin and β-actin...
December 2017: Translational Oncology
https://www.readbyqxmd.com/read/29079795/comprehensive-single-cell-transcriptome-analysis-reveals-heterogeneity-in-endometrioid-adenocarcinoma-tissues
#18
Shinichi Hashimoto, Yuta Tabuchi, Hideaki Yurino, Yoshihiko Hirohashi, Shungo Deshimaru, Takuya Asano, Tasuku Mariya, Kenshiro Oshima, Yuzuru Takamura, Yoshiaki Ukita, Akio Ametani, Naoto Kondo, Norikazu Monma, Tadayuki Takeda, Sadahiko Misu, Toshitugu Okayama, Kazuho Ikeo, Tsuyoshi Saito, Shuich Kaneko, Yutaka Suzuki, Masahira Hattori, Kouji Matsushima, Toshihiko Torigoe
Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29074615/estrogen-is-an-important-mediator-of-mast-cell-activation-in-ovarian-endometriomas
#19
Tianhong Zhu, Shaojie Ding, Tiantian Li, Libo Zhu, Xiufeng Huang, Xinmei Zhang
Endometriosis is an estrogen-dependent disease. Previous research has shown that abnormal enzymes associated with estrogen (E2) metabolism and an increased number of mast cells (MCs) in endometriomas are implicated in pathogenesis of endometriosis. However, it remains unclear how MCs mediate the role of E2 in endometriosis. Accordingly, we investigated whether E2 was associated with the number of MCs, and the rate of degranulation, in local ovarian endometriomas, as well as the role of E2 on MCs during the pathogenesis of endometriosis...
October 26, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29049924/equine-mesenchymal-stem-cells-derived-from-endometrial-or-adipose-tissue-share-significant-biological-properties-but-have-distinctive-pattern-of-surface-markers-and-migration
#20
J Cabezas, D Rojas, F Navarrete, R Ortiz, G Rivera, F Saravia, L Rodriguez-Alvarez, F O Castro
Adult stromal mesenchymal stem cells (MSCs) have been postulated as responsible for cell renewal in highly and continuously regenerative tissues such as the endometrium. MSCs have been identified in the endometrium of many species including humans, rodents, pets and some farm animals, but not in horses. The objective of this work was to isolate such cells from the endometrium of mares and to compare their main biological attributes with horse adipose-derived MSCs. Here we successfully isolated and characterized endometrial MSCs (eMSCs) from mares...
October 6, 2017: Theriogenology
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