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endometrial stem cell

B Elisabeth Rink, Juliane Kuhl, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
Mesenchymal stem or stromal cells (MSCs) play key roles in tissue homeostasis. In the cyclic equine endometrium, this may be regulated by changes in serum concentrations of sex steroid hormones. This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 h and 7days post-partum; n = 5 per stage) and were analyzed by RT-qPCR...
May 4, 2018: Theriogenology
Lei Chen, Shilei Guo, Cui Wei, Honglan Li, Haiya Wang, Yan Xu
Stem cell transplantation has been considered a promising therapeutic approach for premature ovarian failure (POF). However, to date, no quantitative data analysis of stem cell therapy for POF has been performed. Therefore, the present study performed a meta-analysis to assess the efficacy of stem cell transplantation in improving ovarian function in animal models of POF. In addition, a case report of a patient with POF subjected to stem cell treatment was included to demonstrate that stem cell therapy also contributes to the recovery of ovarian function in patients...
May 2018: Experimental and Therapeutic Medicine
Georgios Theophilou, Camilo L M Morais, Diane E Halliwell, Kássio M G Lima, Josephine Drury, Pierre L Martin-Hirsch, Helen F Stringfellow, Dharani K Hapangama, Francis L Martin
The cyclical process of regeneration of the endometrium suggests that it may contain a cell population that can provide daughter cells with high proliferative potential. These cell lineages are clinically significant as they may represent clonogenic cells that may also be involved in tumourigenesis as well as endometriotic lesion development. To determine whether the putative stem cell location within human uterine tissue can be derived using vibrational spectroscopy techniques, normal endometrial tissue was interrogated by two spectroscopic techniques...
May 9, 2018: Analytical and Bioanalytical Chemistry
Nahid Lorzadeh, Nastaran Kazemirad
Stem cells are a population of undifferentiated cells that originated from embryonic and fetal stages of development. Stem cells possess the potency to produce cells that ultimately form different tissues and organs. They are considered as potential alternative novel therapeutic agents for patients suffering from infertility due to their unlimited source and high differentiation potency. There are several studies that have shown that different types of stem cells (mesenchymal stem cells, embryonic, endometrial, and ovarian stem cells) can be differentiated into both spermatozoa and oocytes in vitro, proving their potential clinical use in management and cure of infertility issues...
May 1, 2018: American Journal of Perinatology
Noemi Eiro, Maria Fraile, Jose Schneider, Francisco Vizoso
Stem/progenitor cells for cell therapy and regenerative medicine should ideally be available in large numbers, after having been isolated using minimally invasive or non-invasive methodologies. Also, they should exhibit wide differentiation potential into multiple lineages, as well as capability to be used successfully in autologous or allogeneic transplantation, and all this in accordance with the applicable guidelines of good manufacturing practice. Thus, the identification and characterization of alternative sources of mesenchymal stem cells (MSCs) is of great importance...
April 26, 2018: Current Stem Cell Research & Therapy
S Darzi, J A Deane, C A Nold, S E Edwards, D J Gough, S Mukherjee, S Gurung, K S Tan, A V Vashi, J A Werkmeister, C E Gargett
The immunomodulatory properties of human endometrial mesenchymal stem cells (eMSC) have not been well characterised. Initial studies showed that eMSC modulated the chronic inflammatory response to a non-degradable polyamide/gelatin mesh in a xenogeneic rat skin wound repair model, but the mechanism remains unclear. In this study, we investigated the immunomodulatory effect of eMSC on the macrophage response to polyamide/gelatin composite mesh in an abdominal subcutaneous wound repair model in C57BL6 immunocompetent and NSG (NOD-Scid-IL2Rgamma null ) immunocompromised mice to determine whether responses differed in the absence of an adaptive immune system and NK cells...
April 26, 2018: Scientific Reports
N Kikalishvili, R Beriashvili, T Muzashvili, G Burkadze
Endometrial neoplasia is the most common malignant tumor of female genital system in developed countries. The incidence of endometrial cancer has increased in the last years and despite advances in diagnosis and treatment, the death rates have steadily been increasing over the past 20 years. Therefore aspects of endometrial cancer development, pathogenesis and effective treatment is especially urgent to this day, as much of the risk for endometrial cancer development is influenced by the environment and lifestyle...
March 2018: Georgian Medical News
Yanli Liu, Fen Yang, Shengying Liang, Qing Liu, Sulei Fu, Zhenyu Wang, Ciqing Yang, Juntang Lin
Peripheral nerve injuries are typically caused by either trauma or medical disorders, and recently, stem cell-based therapies have provided a promising treatment approach. Menstrual blood-derived endometrial stem cells (MenSCs) are considered an ideal therapeutic option for peripheral nerve repair due to a noninvasive collection procedure and their high proliferation rate and immunological tolerance. Here, we successfully isolated MenSCs and examined their biological characteristics including their morphology, multipotency, and immunophenotype...
2018: Stem Cells International
Yijing Chu, Yan Wang, Wei Peng, Lin Xu, Meixin Liu, Jing Li, Xiaoyu Hu, Yan Li, Jianxin Zuo, Yuanhua Ye
Endometrial cancer is the most common gynaecological cancer, and its incidence is increasing. Obesity is a well-recognized risk factor for endometrial cancer, and the mechanisms by which adipose tissue influences tumour development remain controversial. In this study, we examined the high IL-6 level in the ADSCs supernatant following treatment of endometrial cancer cell CM. Then, the activation of STAT3, a major tumourigenic IL-6 effector, was examined in endometrial cancer cells. Conditioned ADSC medium was used to stimulate endometrial cancer cell growth in vitro...
April 20, 2018: Biochemical and Biophysical Research Communications
Farnaz Ghobadi, Farhad Rahmanifar, Davood Mehrabani, Amin Tamadon, Mehdi Dianatpour, Shahrokh Zare, Iman Razeghian Jahromi
Background: Endometrial mesenchymal stem stromal cells (EnMSCs) are critical for uterine function, repair, and regeneration. Objective: This study introduced isolation technique of EnMSCs and compared the characteristics of EnMSCs in mature and immature ewes. Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes were collected from the slaughterhouse. Endometrial cells were isolated from tissue using cold incubation and then chopping and treating was performed with collagenase type I...
February 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
Michael Simoni, Hugh S Taylor
PURPOSE OF REVIEW: The current review provides an update on recent advances in stem cell biology relevant to female reproduction. RECENT FINDINGS: Stem cells are undifferentiated cells that often serve as a reservoir of cells to regenerate tissue in settings or injury or cell loss. The endometrium has progenitor stem cells that can replace all of the endometrium during each menstrual cycle. In addition, multipotent endometrial cells replace these progenitor cells when depleted...
April 12, 2018: Current Opinion in Obstetrics & Gynecology
N Tempest, A M Baker, N A Wright, D K Hapangama
STUDY QUESTION: Is human endometrial leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) gene expression limited to the postulated epithelial stem cell niche, stratum basalis glands, and is it hormonally regulated? SUMMARY ANSWER: LGR5 expressing cells are not limited to the postulated stem cell niche but LGR5 expression is hormonally regulated. WHAT IS KNOWN ALREADY: The human endometrium is a highly regenerative tissue; however, endometrial epithelial stem cell markers are yet to be confirmed...
April 10, 2018: Human Reproduction
Michaël Noë, Ayse Ayhan, Tian-Li Wang, Ie-Ming Shih
The pathogenesis of endometriosis, a common benign but debilitating disease in women, remains elusive. The currently held stem cell theory posits that circulating progenitor/stem cells are deposited outside the uterus where they differentiate into endometrial stroma and glandular tissue to establish endometriosis. Fundamental to testing this hypothesis is to elucidate the evolution of both tissue types. Here, we applied droplet digital PCR to analyze synonymous and missense somatic passenger mutations, which are neutral with respect to clonal selection, among six non-superficial endometriotic lesions...
March 31, 2018: Journal of Pathology
Mojdeh Salehi Namini, Neda Bayat, Roxana Tajerian, Somayeh Ebrahimi-Barough, Mahmoud Azami, Shiva Irani, Saranaz Jangjoo, Sadegh Shirian, Jafar Ai
BACKGROUND: An engineered tissue structure is an artificial scaffold combined with cells and signaling factors. Among various polymers, the polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties. The aim of this study was to study the behavior of human endometrial stem cell (hEnSC)-derived osteoblast cells cultured on PLGA/HA nanocomposite scaffolds. METHODS: hEnSCs were isolated and exposed to osteogenic media for 21 days...
March 27, 2018: Journal of Orthopaedic Surgery and Research
Nuria López-Pérez, Claudia Gil-Sanchis, Hortensia Ferrero, Amparo Faus, Ana Díaz, Antonio Pellicer, Irene Cervelló, Carlos Simón
Endometrial regeneration has long been proposed to be mediated by stem cells, but the isolation of endometrial stem cells has been hampered by a lack of validated markers. Specific markers would enable isolation of these stem cells, thereby promoting advancements in regenerative medicine for the treatment of endometrial diseases and dysfunctions. We sought to investigate the regenerative ability of human endometrial positive for sushi domain containing 2/intercellular adhesion molecule 1 (SUSD2+ /ICAM1+ ) cells and Side Population cell lines in a xenograft mice model...
January 1, 2018: Reproductive Sciences
Ramyar Azizi, Leili Aghebati-Maleki, Mohammad Nouri, Faroogh Marofi, Sohrab Negargar, Mehdi Yousefi
The endometrium is one of the essential components of the uterus. The endometrium of human is a complex and dynamic tissue, which undergoes periods of growth and turn over during any menstrual cycle. Stem cells are initially undifferentiated cells that display a wide range of differentiation potential with no distinct morphological features. Stem cell therapy method recently has become a novel procedure for treatment of tissue injury and fibrosis in response to damage. Currently, there is massive interest in stem cells as a novel treatment method for regenerative medicine and more specifically for the regeneration of human endometrium disorder like Asherman syndrome (AS) and thin endometrium...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ran Zhang, Jinwen Jiao, Huijun Chu, Hongjuan Yang, Liming Wang, Nana Gao
Double primary endometrioid endometrial and ovarian carcinomas (DPEEOCs) are the most common multiple gynecological carcinomas. In recent years, gene sequential comparison analysis has strongly supported the opinion that sporadic double endometrioid endometrial and ovarian cancers (DEEOCs) are clonally related in both primary and metastatic tumors. In order to find more clonal evidence for DPEEOC, we investigated cancer stem cells (CSCs). SOX2 and OCT4 are two common factors in CSCs. MicroRNA (miRNA)-145, a small non-coding RNA, has effects in regulating gene expression and tumorigenesis in CSCs...
March 23, 2018: Histology and Histopathology
Qin Zhou, Xixi Wu, Jianguo Hu, Rui Yuan
Intrauterine adhesions (IUAs) are mainly derived from fibrous tissue formation following endometrial damage. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)α and the stromal derived factor (SDF)‑1/C‑X‑C chemokine receptor type 4 (CXCR‑4) axis are abnormally expressed in IUA endometrium. A total of 76 human endometrial biopsy samples (normal, n=20; mild‑to‑moderate IUAs, n=40; and severe IUAs, n=16) were employed, and Sprague‑Dawley rat IUA models at different time points were constructed...
March 22, 2018: International Journal of Molecular Medicine
Javier García-Solares, Jacques Donnez, Olivier Donnez, Marie-Madeleine Dolmans
Adenomyosis is a commonly diagnosed estrogen-dependent gynecological disorder that causes pelvic pain, abnormal uterine bleeding, and infertility. Despite its prevalence and severity of symptoms, its pathogenesis and etiology have not yet been elucidated. The aim of this manuscript is to review the different hypotheses on the origin of adenomyotic lesions and the mechanisms involved in the evolution and progression of the disease. Two main theories have been proposed to explain the origin of adenomyosis. The most common suggests involvement of tissue injury and the repair mechanism and claims that adenomyosis results from invagination of the endometrial basalis into the myometrium...
March 2018: Fertility and Sterility
Inge C Van Gool, Emily Rayner, Elisabeth M Osse, Remi A Nout, Carien L Creutzberg, Ian Tomlinson, David N Church, Vincent T H B M Smit, Niels de Wind, Tjalling Bosse, Mark Drost
PURPOSE: Pathogenic POLE proofreading domain mutations are found in many malignancies where they are associated with ultramutation and favorable prognosis. The extent to which this prognosis depends on their sensitivity to adjuvant treatment is unknown, as is the optimal therapy for advanced-staged or recurrent POLE-mutant cancers. EXPERIMENTAL DESIGN: We examined the recurrence-free survival of women with POLE-mutant and POLE-wild-type endometrial cancers (ECs) in the observation arm of the randomized PORTEC-1 EC trial (N=245 patients with stage I EC for analysis)...
March 20, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
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