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endometrial stem cell

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https://www.readbyqxmd.com/read/29134714/identification-of-perivascular-and-stromal-mesenchymal-stem-progenitor-cells-in-porcine-endometrium
#1
J Wiater, M Niedziela, A Posmysz, K Wartalski, B Gajda, Z Smorąg, Z Rajfur, J Karasiński
Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29117721/enhanced-sciatic-nerve-regeneration-by-human-endometrial-stem-cells-in-an-electrospun-poly-%C3%AE%C2%B5-caprolactone-collagen-nbg-nerve-conduit-in-rat
#2
Forouzan Mohamadi, Somayeh Ebrahimi-Barough, Mohammad Reza Nourani, Korosh Mansoori, Majid Salehi, Ali Akbar Alizadeh, Seyed Mohammad Tavangar, Farshid Sefat, Siavash Sharifi, Jafar Ai
In recent years, for neurodegenerative diseases therapy, research has focused on the stem cells therapy. Due to promising findings in stem cell therapy, there are various sources of stem cells for transplantation in human. The aim of this study was to evaluate sciatic nerve regeneration in the rat after nerve transaction followed by human endometrial stem cells (hEnSCs) treatment into poly (e-caprolactone)/collagen/nanobioglass (PCL/collagen/NBG) nanofibrous conduits. After treatment of animals, the performance in motor and sensory tests, showed significant improvement in rats treated with hEnSCs as an autograft...
November 8, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29106744/germ-cell-tumor-growth-patterns-originating-from-clear-cell-carcinomas-of-the-ovary-and-endometrium-a-comparative-immunohistochemical-study-favouring-their-origin-from-somatic-stem-cells
#3
F F Nogales, J Prat, M Schuldt, N Cruz-Viruel, B Kau, E D'Angelo, X Matias-Guiu, A Vidal, W G McCluggage, J W Oosterhuis
AIMS: To report a series of 11 ovarian and one endometrial neoplasm in elderly patients with mixed clear cell tumour and germ cell tumour (GCT) components, to compare their immunohistochemical and demonstrate a putative stem cell population. METHODS AND RESULTS: The clear cell tumours included 11 clear cell carcinomas (CCC) and 1 borderline clear cell tumour, while the GCT always included glandular yolk sac tumour (YST). In 4 cases, there were also foci of teratoma with immature neuroepithelial and endodermal tissues, and undifferentiated areas showing true embryoids...
November 6, 2017: Histopathology
https://www.readbyqxmd.com/read/29105567/in-vivo-survival-of-human-endometrial-mesenchymal-stem-cells-transplanted-under-the-kidney-capsule-of-immunocompromised-mice
#4
Shanti Gurung, James A Deane, Saeedeh Darzi, Jerome A Werkmeister, Caroline E Gargett
Human endometrial mesenchymal stem cells (eMSCs) are a well-characterised adult stem cell type with potential for use in regenerative medicine or cell-therapy. As a proof of principle, we demonstrated that eMSCs promoted wound healing by reducing the inflammatory response through a paracrine action in a subcutaneous rat model of wound repair. However, an efficient protocol for culturing eMSCs in the undifferentiated state and a reliable method of labelling them for cell tracking were lacking. Here, we investigated the use of a lentiviral vector containing the mCherry fluorescent reporter gene to transduce and label eMSCs following in vitro culturing in A83-01-containing medium, and different methods of tracing the labelled cells following transplantation under the kidney capsule of immunocompromised NSG mice...
November 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29100623/pathogenesis-of-deep-endometriosis
#5
REVIEW
Stephan Gordts, Philippe Koninckx, Ivo Brosens
The pathophysiology of (deep) endometriosis is still unclear. As originally suggested by Cullen, change the definition "deeper than 5 mm" to "adenomyosis externa." With the discovery of the old European literature on uterine bleeding in 5%-10% of the neonates and histologic evidence that the bleeding represents decidual shedding, it is postulated/hypothesized that endometrial stem/progenitor cells, implanted in the pelvic cavity after birth, may be at the origin of adolescent and even the occasionally premenarcheal pelvic endometriosis...
October 31, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/29096715/differentiation-of-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-into-endometrial-cells
#6
Qin Shi, JingWei Gao, Yao Jiang, Baolan Sun, Wei Lu, Min Su, Yunzhao Xu, Xiaoqing Yang, Yuquan Zhang
BACKGROUND: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are a novel and promising strategy for tissue engineering because of their ability to differentiate into many cell types. We characterized the differentiation of WJ-MSCs into endometrial epithelial cell (EEC)-like and endometrial stromal cell (ESC)-like cells and assessed the effect of 17β-estradiol and 8-Br-cAMP on the differentiation system. METHODS: WJ-MSCs were treated in two ways to differentiate into EEC-like and ESC-like cells respectively: cocultured with ESCs in control/differentiation medium (17β-estradiol, growth factors); and cultured in control/differentiation medium (8-Br-cAMP alone or 8-Br-cAMP plus 17β-estrogen and growth factors)...
November 2, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29096246/isolation-of-stem-like-cancer-cells-in-primary-endometrial-cancer-using-cell-surface-markers-cd133-and-cxcr4
#7
Yi Sun, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Fang Wang, Chika Soko, Sigeru Saito, Toshio Nikaido
Endometrial cancer (EC) is the most common familiar gynecologic malignant tumor identified in the female reproductive system and has been increasing yearly. In this study, we will identify the surface markers and stem cell markers related with cancer stem cells (CSCs) of EC. Tissue samples were obtained from endometrial cancer patients during surgical procedures. Single cells were isolated from the tissues for culturing, transfection into nude mice, and histopathology analysis. RT-PCR demonstrated that the cultured cells strongly expressed stemness-related genes, such as c-Myc, Sox-2, Nanog, Oct 4A, ABCG2, BMI-1, CK-18, Nestin and β-actin...
December 2017: Translational Oncology
https://www.readbyqxmd.com/read/29079795/comprehensive-single-cell-transcriptome-analysis-reveals-heterogeneity-in-endometrioid-adenocarcinoma-tissues
#8
Shinichi Hashimoto, Yuta Tabuchi, Hideaki Yurino, Yoshihiko Hirohashi, Shungo Deshimaru, Takuya Asano, Tasuku Mariya, Kenshiro Oshima, Yuzuru Takamura, Yoshiaki Ukita, Akio Ametani, Naoto Kondo, Norikazu Monma, Tadayuki Takeda, Sadahiko Misu, Toshitugu Okayama, Kazuho Ikeo, Tsuyoshi Saito, Shuich Kaneko, Yutaka Suzuki, Masahira Hattori, Kouji Matsushima, Toshihiko Torigoe
Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29074615/estrogen-is-an-important-mediator-of-mast-cell-activation-in-ovarian-endometriomas
#9
Tianhong Zhu, Shaojie Ding, Tiantian Li, Libo Zhu, Xiufeng Huang, Xinmei Zhang
Endometriosis is an estrogen-dependent disease. Previous research has shown that abnormal enzymes associated with estrogen (E2) metabolism and an increased number of mast cells (MCs) in endometriomas are implicated in pathogenesis of endometriosis. However, it remains unclear how MCs mediate the role of E2 in endometriosis. Accordingly, we investigated whether E2 was associated with the number of MCs, and the rate of degranulation, in local ovarian endometriomas, as well as the role of E2 on MCs during the pathogenesis of endometriosis...
October 26, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29049924/equine-mesenchymal-stem-cells-derived-from-endometrial-or-adipose-tissue-share-significant-biological-properties-but-have-distinctive-pattern-of-surface-markers-and-migration
#10
J Cabezas, D Rojas, F Navarrete, R Ortiz, G Rivera, F Saravia, L Rodriguez-Alvarez, F O Castro
Adult stromal mesenchymal stem cells (MSCs) have been postulated as responsible for cell renewal in highly and continuously regenerative tissues such as the endometrium. MSCs have been identified in the endometrium of many species including humans, rodents, pets and some farm animals, but not in horses. The objective of this work was to isolate such cells from the endometrium of mares and to compare their main biological attributes with horse adipose-derived MSCs. Here we successfully isolated and characterized endometrial MSCs (eMSCs) from mares...
October 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/29040564/n-cadherin-identifies-human-endometrial-epithelial-progenitor-cells-by-in-vitro-stem-cell-assays
#11
Hong P T Nguyen, L Xiao, James A Deane, Ker-Sin Tan, Fiona L Cousins, Hirotaka Masuda, Carl N Sprung, Anna Rosamilia, Caroline E Gargett
STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? SUMMARY ANSWER: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium...
November 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29024986/stromal-fibroblasts-from-perimenopausal-endometrium-exhibit-a-different-transcriptome-than-those-from-the-premenopausal-endometrium
#12
David W Erikson, Fatima Barragan, Terhi T Piltonen, Joseph C Chen, Shaina Balayan, Juan C Irwin, Linda C Giudice
Human endometrium undergoes extensive regeneration on a cyclic basis in premenopausal women and likely occurs through the contribution of stem/progenitor cells. Menopause results in the permanent cessation of menstrual cycles and is preceded by perimenopause, a period of several years in which endocrine and biological changes occur and is a period of risk for endometrial proliferative disorders. The objectives of this study were to identify endometrial mesenchymal stem cells (eMSC) and endometrial stromal fibroblasts (eSF) in endometrium of perimenopausal women and perform expression profile analysis of perimenopausal eMSC and eSF to gain insight into the biology of stem/progenitor and lineage cell populations during the transition to menopause...
September 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024798/hysteroscopic-management-of-asherman-s-syndrome-special-issue-report-on-uterine-surgery-in-the-journal-of-minimally-invasive-gynecology
#13
Zaraq Khan, Jeffrey M Goldberg
In developed countries, Asherman's Syndrome is almost always the result of a prior intrauterine operative trauma. This is often asymptomatic but may result in hypo- or amenorrhea and can contribute to infertility and pregnancy complications. We will review their etiology, clinical implications, and the systems which have been proposed to classify their extent. The numerous methods reported for performing lysis of intrauterine adhesions will be summarized along with the clinical results. Current strategies to prevent recurrence of intrauterine adhesions have not been conclusively shown to be clinically effective but the potential for endometrial regeneration using stem cells is an exciting modality under investigation...
October 9, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28990139/association-between-morphological-patterns-of-myometrial-invasion-and-cancer-stem-cell-markers-in-endometrial-endometrioid-carcinoma
#14
Ji Y Park, Daegy Hong, Ji Young Park
In endometrial endometrioid adenocarcinoma (EEC), the depth of myometrial invasion (MI) is an important parameter for determining whether additional treatment is warranted. The present study investigated the association between MI patterns, cancer stem cell (CSC) phenotypes, and their clinicopathological significance in EEC. A total of 73 cases of EEC with MI were examined in this study. Haematoxylin and eosin-stained tissue specimens were analysed for MI pattern, which was categorised as infiltrating; expansile; adenomyosis (AM)-like; or microcystic, elongated, and fragmented (MELF)-type...
October 8, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28990049/mirna%C3%A2-34a%C3%A2-5p-downregulation-of-vegfa-in-endometrial-stem-cells-contributes-to-the-pathogenesis-of-endometriosis
#15
Ying Ma, Yu-Xin Huang, Yan-Ying Chen
Endometrial-derived stem cells (EnSCs) serve an important role in the development of endometriosis via retrograde menstruation. Abnormal expression of miRNAs in EnSCs is involved in the etiology of endometriosis, however, the mechanisms remain unclear. The aim of the present study was to investigate the expression of miR‑34a‑5p and VEGFA in endometrial samples from patients with or without endometriosis, and then examine the underlying mechanism of microRNA‑34a‑5p regulation of VEGFA in EnSCs. Endometrial samples from patients with or without endometriosis were collected, and miR‑34a‑5p expression in the two groups was measured using RT‑PCR...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28972492/age-related-specificities-of-endometrial-proliferation-stem-cell-index-distribution
#16
N Kikalishvili, R Beriashvili, T Muzashvili, G Burkadze
Endometrial stem cells take the special place among somatic stem cells of female reproductive system, since their essence and nature still remains unclear - cellular complexity of endometrium, its hormone-dependence and cycling remodeling makes the study of endometrial stem cells exclusively complicated. Consequently, the detection of endometrial stem cells and identification of their location in the complex cellular hierarchy still remains challenging. The aim of our study was to explore the age-related specificities of endometrial proliferative / stem cell index distribution under normal and hyperplasia conditions...
September 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28953502/endometrial-cancers-harboring-mutated-fibroblast-growth-factor-receptor-2-protein-are-successfully-treated-with-a-new-small-tyrosine-kinase-inhibitor-in-an-orthotopic-mouse-model
#17
Sebastien Taurin, Chieh-Hsiang Yang, Maria Reyes, Sungpil Cho, Demetrius M Coombs, Elke A Jarboe, Theresa L Werner, C Matthew Peterson, Margit M Janát-Amsbury
OBJECTIVES: AL3818 (anlotinib) is a receptor tyrosine kinase inhibitor targeting vascular endothelial growth factor receptors (VEGFR1, VEGFR2/KDR, and VEGFR3), stem cell factor receptor (C-kit), platelet-derived growth factor (PDGFβ), and fibroblast growth factor receptors (FGFR1, FGFR2, and FGFR3). This study evaluates the efficacy of AL3818 studying tumor regression in an orthotopic murine endometrial cancer model. METHODS: We tested the cytotoxicity of AL3818 on a panel of 7 human endometrial cancer cell lines expressing either wild-type or mutant FGFR2 and also assessed the in vivo antitumor efficacy in a murine, orthotopic AN3CA endometrial cancer model...
September 26, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28941322/stromal-cell-derived-factor-1-mediates-cardiac-allograft-tolerance-induced-by-human-endometrial-regenerative-cell-based-therapy
#18
Xu Lan, Grace Wang, Xiaoxi Xu, Shanzheng Lu, Xiang Li, Baoren Zhang, Ganggang Shi, Yiming Zhao, Caigan Du, Hao Wang
Endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells, and their therapeutic potential has been tested in the prevention of renal ischemic reperfusion injury, acute liver injury, ulcerative colitis, and immunosuppression. However, their potential in the induction of transplant tolerance has not been investigated. The present study was undertaken to investigate the efficacy of ERCs in inducing cardiac allograft tolerance and the function of stromal cell-derived factor-1 (SDF-1) in the ERC-mediated immunoregulation...
November 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28913973/bone-marrow-stem-cells-do-not-contribute-to-endometrial-cell-lineages-in-chimeric-mouse-models
#19
Yih Rue Ong, Fiona L Cousins, Xiaoqing Yang, Ahmed Aedh A Al Mushafi, David T Breault, Caroline E Gargett, James A Deane
Studies from five independent laboratories conclude that bone marrow stem cells transdifferentiate into endometrial stroma, epithelium, and endothelium. We investigated the nature of bone marrow-derived cells in the mouse endometrium by reconstituting irradiated wild type recipients with bone marrow containing transgenic mTert-green fluorescent protein (GFP) or chicken β-actin (Ch β-actin)-GFP reporters. mTert-GFP is a telomerase marker identifying hematopoietic stem cells and subpopulations of epithelial, endothelial, and immune cells in the endometrium...
September 15, 2017: Stem Cells
https://www.readbyqxmd.com/read/28883083/neupogen-and-mesenchymal-stem-cells-are-the-novel-therapeutic-agents-in-regeneration-of-induced-endometrial-fibrosis-in-experimental-rats
#20
Dina Sabry, Abeer Mostafa, Samar Marzouk, Walaa Ibrahim, Hanan H M Ali, Aymen Hassan, Ashraf Shamaa
Endometrial fibrosis is the presence of intrauterine adhesions (IUAs) after any uterine surgery or curettage and it results in infertility and recurrent pregnancy loss. We evaluated the role of human mesenchymal stem cells (hMSCs) as a therapeutic agent of endometrial fibrosis. We also compared the effect of MSCs with the effect of estrogen and neupogen either each alone or as a combined therapy with MSCs. This experimental study was performed on 84 albino rats which were divided into seven groups (n=12 rats/group) as follows, group1: normal control rats, group 2: induced fibrosis, group 3: induced fibrosis that received oral estrogen, group 4: induced fibrosis that received hMSCs, group 5: induced fibrosis that received hMSCs and estrogen, group 6: induced fibrosis that received neupogen, and group 7: induced fibrosis that received hMSCs and neupogen...
October 31, 2017: Bioscience Reports
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