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endometrial stem cell

Tatsanee Phermthai, Kittima Tungprasertpol, Suphakde Julavijitphong, Puttachart Pokathikorn, Sasiprapa Thongbopit, Suparat Wichitwiengrat
Transplantation of mesenchymal stem cells (MSC) can effectively repair endometrial deficiencies, including infertile patients with a problem of inadequate endometrium thickness. Although, MSC derived from different organ sources have a similarity of MSC specific characteristics, endometrial stem cells (EMSC) are temporally regulated throughout the menstrual cycle in a micro-environmental niche found only in endometrial tissue. Given the micro-environment niche, developing treatments for endometrial disorders with EMSC should be a top priority...
October 21, 2016: Reproductive Biology
Shanzheng Lu, Ganggang Shi, Xiaoxi Xu, Grace Wang, Xu Lan, Peng Sun, Xiang Li, Baoren Zhang, Xiangying Gu, Thomas E Ichim, Hao Wang
BACKGROUND: The endometrial regenerative cell (ERC) is a novel type of adult mesenchymal stem cell isolated from menstrual blood. Previous studies demonstrated that ERCs possess unique immunoregulatory properties in vitro and in vivo, as well as the ability to differentiate into functional hepatocyte-like cells. For these reasons, the present study was undertaken to explore the effects of ERCs on carbon tetrachloride (CCl4)-induced acute liver injury (ALI). METHODS: An ALI model in C57BL/6 mice was induced by administration of intraperitoneal injection of CCl4...
October 22, 2016: Journal of Translational Medicine
C Yazbeck
No abstract text is available yet for this article.
October 14, 2016: Gynécologie, Obstétrique & Fertilité
Irene Moridi, Ramanaiah Mamillapalli, Emine Cosar, Gulcin Sahin Ersoy, Hugh S Taylor
Endometriosis is an inflammatory gynecological disorder caused by the growth of endometrial tissue outside the uterus. Endometriosis produces chemokines, including CXCL12, that attract bone marrow cells to the lesions. In this study, we describe the expression, localization, and chemotactic activity of CXCL12 in endometriotic lesions. Biopsies were collected both from women with endometriosis undergoing laparoscopy and control endometrium from women without endometriosis. Expression of CXCl12 and CXCR4 messenger RNA was increased approximately 4- and 6-fold, respectively, in endometriosis compared to eutopic endometrium...
October 11, 2016: Reproductive Sciences
Carolina Nogueira de Moraes, Leandro Maia, Marianne Camargos Dias, Camila P Freitas Dell'Aqua, Ligia Souza Lima Silveira da Mota, Aspinas Chapwanya, Fernanda da Cruz Landim-Alvarenga, Eunice Oba
Endometrial mesenchymal stem/progenitor cells (eMSCs) are multipotent cells known to modulate the immune system and have clinical application for human and animal health. This makes these bovines cells attractive for dual use as cellular therapy and experimental model. The aim of this study was to isolate, evaluate the differentiation potential, immunophenotypic and immunocytochemistry characteristics, chromosomal stability, cloning efficiency, and cryopreservation response of bovine eMSCs collected in two phases of the estrous cycle...
October 4, 2016: Cell Biology International
Alisa P Domnina, Polina V Novikova, Olga G Lyublinskaya, Valeriy V Zenin, Irina I Fridlyanskaya, Vyacheslav M Mikhailov, Nikolay N Nikolsky
Stem cell transplantation, which is based on the application of mesenchymal stem/stromal cells (MSCs), is a rapidly developing approach to the regenerative therapy of various degenerative disorders characterized by brain and heart failure, as well as skin lesions. In comparison, the use of stem cell transplantations to treat infertility has received less attention. One of the causes of miscarriages and fetal growth delay is the loss of the decidual reaction of endometrial cells. The present study modeled decidualization processes in pseudopregnant rats...
October 2016: Experimental and Therapeutic Medicine
Farzaneh Khademi, Jafar Ai, Masoud Soleimani, Javad Verdi, Seyed Mohammad Tavangar, Esmaeil Sadroddiny, Mohammad Massumi, Seyed Mahmoud Hashemi
Liver tissue engineering (TE) is rapidly emerging as an effective technique which combines engineering and biological processes to compensate for the shortage of damaged or destroyed liver tissues. We examined the viability, differentiation, and integration of hepatocyte-like cells on an electrospun polyethersulfone (PES) scaffold, derived from human endometrial stem cells (hEnSCs). Natural polymers were separately grafted on plasma-treated PES nanofibers, that is, collagen, heparan sulfate (HS) and collagen-HS...
September 30, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Naghmeh Bahrami, Fatemeh Malekolkottab, Somayeh Ebrahimi-Barough, Zahra Alizadeh Tabari, Jalaleddin Hamisi, Ahmadreza Kamyab, Abdolreza Mohamadnia, Armin Ai, Farshad Bayat, Naeim Bahrami, Jafar Ai
We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group...
September 30, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Yeonwoo Park, Mauris C Nnamani, Jamie Maziarz, Günter P Wagner
Studies in human and mouse have shown that decidual stromal cells (DSC), which develop in the innermost lining of uterus, mediate placentation by regulating maternal immune response against the fetus and the extent of fetal invasion. Investigating when and how DSC evolved is thus a key step to reconstructing the evolutionary history of mammalian pregnancy. We present molecular evidence placing the origin of DSC in the stem lineage of eutherians (extant placental mammals). The transcription factor forkhead box O1 (FOXO1) is a part of the core regulatory transcription factor complex (CoRC) that establishes the cell type identity of DSC...
September 15, 2016: Molecular Biology and Evolution
Amber A Bokhari, Tabari M Baker, Batsukh Dorjbal, Sana Waheed, Christopher M Zahn, Chad A Hamilton, G Larry Maxwell, Viqar Syed
Nestin, an intermediate filament protein and a stem cell marker is expressed in several tumors. Until recently, little was known about the expression levels and the role of Nestin in endometrial cancer. Compared to the immortalized endometrial epithelial cell line EM-E6/E7-TERT, endometrial cancer cell lines express high to moderate levels of Nestin. Furthermore, endometrial tumors and tumor cell lines have a cancer stem-like cell subpopulation expressing CD133. Among the cancer lines, AN3CA and KLE cells exhibited both a significantly higher number of CD133+ cells and expressed Nestin at higher levels than Ishikawa cells...
September 10, 2016: Oncotarget
Panieh Terraf, Shideh Montasser Kouhsari, Jafar Ai, Hamideh Babaloo
Loss of motor and sensory function as a result of neuronal cell death and axonal degeneration are the hallmarks of spinal cord injury. To overcome the hurdles and achieve improved functional recovery multiple aspects, it must be taken into account. Tissue engineering approaches by coalescing biomaterials and stem cells offer a promising future for treating spinal cord injury. Here we investigated human endometrial stem cells (hEnSCs) as our cell source. Electrospun poly ε-caprolactone (PCL) scaffolds were used for hEnSC adhesion and growth...
September 13, 2016: Molecular Neurobiology
Jianmei Wang, Baohui Ju, Caijun Pan, Yan Gu, Yu Zhang, Li Sun, Bumei Zhang, Yujuan Zhang
AIMS: To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on intrauterine adhesions (IUA). METHODS: BMSCs were isolated and labeled by green fluorescence protein. IUA model was established by mechanical injury. 48 rats were randomly divided into control, IUA model, BMSCs vein injection and BMSCs intrauterine injection groups (n=12 in each group). The third generation of BMSCs was injected through tail vein or intrauterine...
2016: Cellular Physiology and Biochemistry
Zahra Barabadi, Mahmoud Azami, Esmaeel Sharifi, Roya Karimi, Nasrin Lotfibakhshaiesh, Reza Roozafzoon, Mohammad Taghi Joghataei, Jafar Ai
Selecting suitable cell sources and angiogenesis induction are two important issues in myocardial tissue engineering. Human endometrial stromal cells (EnSCs) have been introduced as an abundant and easily available resource in regenerative medicine. Bioactive glass is an agent that induces angiogenesis and has been studied in some experiments. The aim of this study was to investigate in vitro differentiation capacity of endometrial stem cells into cardiomyocyte lineage and to evaluate capability of bioactive glass nanoparticles toward EnSCs differentiation into endothelial lineage and angiogenesis on hydrogel scaffold...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
X-J Liu, X-G Bai, Y-L Teng, L Song, N Lu, R-Q Yang
OBJECTIVE: miRNAs have been recently reported to contribute to the etiology of endometriosis in stem cells. However, the mechanisms remain unclear. The aim of this investigation is to explore the expression of miR-15a-5p and VEGFA in endometrial samples from patients with or without endometriosis. And then examine the regulation by miR-15a-5p on the expression of VEGFA. PATIENTS AND METHODS: Here we collected 31 endometrial samples from patients with or without endometriosis and characterized the miRNAs expression profiles of these two groups...
August 2016: European Review for Medical and Pharmacological Sciences
Dongmei Lai, Ying Guo, Qiuwan Zhang, Yifei Chen, Charlie Xiang
Human endometrial mesenchymal stem cells (EnSCs) derived from menstrual blood are a unique stem cell source. Evidence suggests that EnSCs exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine. However, the potential of EnSCs to differentiate into germline cells in vitro remains unclear. In this study, EnSCs were induced to differentiate into germ cells in a differentiation medium supplemented with 20% human follicular fluid. Our results demonstrated that EnSCs derived from human menstrual blood form oocyte-like cells and express germ cell markers...
September 2, 2016: Acta Biochimica et Biophysica Sinica
Eun-Yeong Kim, Hee-Jung Choi, Tae-Wook Chung, Jun-Yong Choi, Hyung Sik Kim, Yeon-Seop Jung, Syng-Ook Lee, Ki-Tae Ha
The leaves and stems of Perilla frutescens var. acuta Kudo (PF) have been used to prevent threatened abortion in traditional medicine in the East Asian countries. Because reduced receptivity of endometrium is a cause of abortion, we analyzed the action of PF on the endometrial receptivity. PF increased the level of leukemia inhibitory factor (LIF), a major cytokine regulating endometrial receptivity, and LIF receptor in human endometrial Ishikawa cells. The PF-induced LIF expression was mediated by c-jun N-terminal kinase (JNK) and p38 pathways...
August 2016: Journal of Pharmacological Sciences
Mehri Fayazi, Mojdeh Salehnia, Saeideh Ziaei
BACKGROUND: Stem cell factor (SCF) is a transcriptional factor which plays crucial roles in normal proliferation, differentiation and survival in a range of stem cells. OBJECTIVE: The aim of the present study was to examine the proliferation effect of different concentrations of SCF on expansion of human endometrial CD146(+) cells. MATERIALS AND METHODS: In this experimental study, total populations of isolated human endometrial suspensions after fourth passage were isolated by magnetic activated cell sorting (MACS) into CD146(+) cells...
July 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
Nour Alowayed, Madhuri S Salker, Ni Zeng, Yogesh Singh, Florian Lang
BACKGROUND: LEFTY2, a suppressor of cell proliferation, tumor growth, regulator of stemness and embryonic differentiation, is a negative regulator of cancer cell reprogramming. Malignant transformation may lead to migration requiring loss of adhesion and gain of migratory activity. Signaling involved in the orchestration of migration, proliferation and spreading of cells include focal adhesion kinase (FAK) and adhesion molecule E-cadherin. AIMS: The present study explored whether LEFTY2 influences the proliferation marker MKi67, FAK activity, E-cadherin abundance and migration of Ishikawa human endometrial carcinoma cells...
2016: Cellular Physiology and Biochemistry
Yanling Zhang, Xiaona Lin, Yongdong Dai, Xiaoxiao Hu, Haiyan Zhu, Yinshen Jiang, Songying Zhang
Intrauterine adhesions are common acquired endometrial syndromes secondary to endometrial injury, with limited effective therapies. Recently, several studies have reported that bone marrow stem cells (BMSCs) could repair injured endometrium in animal experiments. However, the role of stem cells in endometrial injury repair and its therapeutic mechanisms remain unclear. Here, we established mouse endometrial injury model and examined the benefit of human endometrial mesenchymal stem cells derived from menstrual blood (MenSCs) in restoration of injured endometrium...
November 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Xiawei Li, Zhiying Li, Dongxia Hou, Yuhang Zhao, Chen Wang, Xueling Li
Endometrial epithelial cells (EECs) cultured in vitro are valuable tools for investigating embryo implantation and trophoblast differentiation. In this study, we have established the bovine EECs and trophoblast stem-like (TS) coculture system, and used it to investigate the binucleate cell formation of ungulates. The EECs was derived from the uterine horn ipsilateral to the corpus luteum by using collagenase I and deoxyribonuclease I, which exhibited typical epithelial morphology and were expressing bovine uterine epithelial marker such as IFNAR1, IFNAR2, Erα, PGR, ESR1 and KRT18...
July 29, 2016: Cytotechnology
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