keyword
MENU ▼
Read by QxMD icon Read
search

Stability engineering

keyword
https://www.readbyqxmd.com/read/28822812/determinants-of-thermostability-in-the-cytochrome-p450-fold
#1
REVIEW
Kurt L Harris, Raine E S Thomson, Silja J Strohmaier, Yosephine Gumulya, Elizabeth M J Gillam
Cytochromes P450 are found throughout the biosphere in a wide range of environments, serving a multitude of physiological functions. The ubiquity of the P450 fold suggests that it has been co-opted by evolution many times, and likely presents a useful compromise between structural stability and conformational flexibility. The diversity of substrates metabolized and reactions catalyzed by P450s makes them attractive starting materials for use as biocatalysts of commercially useful reactions. However, process conditions impose different requirements on enzymes to those in which they have evolved naturally...
August 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28821766/protein-composition-analysis-of-polyhedra-matrix-of-bombyx-mori-nucleopolyhedrovirus-bmnpv-showed-powerful-capacity-of-polyhedra-to-encapsulate-foreign-proteins
#2
Zhong-Jian Guo, Meng-Han Yu, Xian-Yun Dong, Wei-Li Wang, Ting Tian, Xian-Yin Yu, Xu-Dong Tang
Polyhedra can encapsulate other proteins and have potential applications as protein stabilizers. The extremely stable polyhedra matrix may provide a platform for future engineered micro-crystal devices. However, the protein composition of the polyhedra matrix remains largely unknown. In this study, the occlusion-derived virus (ODV)-removed BmNPV polyhedra matrix fraction was subjected to SDS-PAGE and then an LC-ESI-MS/MS analysis using a Thermo Scientific Q Exactive mass spectrometer. In total, 28 host and 91 viral proteins were identified...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821545/heterologous-production-of-thermophile-thermochromatium-tepidum-photosynthetic-reaction-centre-and-light-harvesting-1-complexes-in-the-mesophile-rhodobacter-sphaeroides-and-thermal-stabilities-of-a-hybrid-core-complex
#3
D Jun, V Huang, J T Beatty
The photosynthetic complexes of the thermophile Thermochromatium tepidum are of considerable interest in biohybrid solar cell applications because of the capability of thermophilic proteins to tolerate elevated temperatures. Synthetic operons encoding reaction centre (RC) and light harvesting 1 (LH1) pigment-protein complexes of T. tepidum were expressed in the mesophile Rhodobacter sphaeroides The T. tepidum RC (TRC) was assembled, and functional with the addition of menadione to populate the QA pocket. The production of the T...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28820969/a-disulfide-stabilized-human-vl-single-domain-antibody-library-is-a-source-of-soluble-and-highly-thermostable-binders
#4
Kevin A Henry, Hiba Kandalaft, Michael J Lowden, Martin A Rossotti, Henk van Faassen, Greg Hussack, Yves Durocher, Dae Young Kim, Jamshid Tanha
We have previously shown that incorporation of a second intradomain disulfide linkage into camelid VHH and human VH/VL single-domain antibodies confers increased thermostability. Here, we explored the effects of introducing an additional disulfide linkage, formed between Cys48 and Cys64 (Kabat numbering), into a phage-displayed synthetic human VL library. In comparison to an identical library bearing only the highly conserved Cys23-Cys88 disulfide linkage, the disulfide-stabilized VL library tolerated a similar degree of randomization but retained a higher level of functional diversity after selection with protein L...
August 15, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28819027/combination-therapy-with-bispecific-antibodies-and-pd-1-blockade-enhances-the-antitumor-potency-of-t-cells
#5
Chien-Hsing Chang, Yang Wang, Rongxiu Li, Diane L Rossi, Donglin Liu, Edmund A Rossi, Thomas M Cardillo, David M Goldenberg
The DOCK-AND-LOCK (DNL®) method is a platform technology that combines recombinant engineering and site-specific conjugation to create multispecific, multivalent antibodies of defined composition with retained bioactivity. We have applied DNL® to generate a novel class of trivalent bispecific antibodies (bsAbs), each comprising an anti-CD3 scFv covalently conjugated to a stabilized dimer of different anti-tumor Fabs. Here we report the further characterization of two such constructs, (E1)-3s and (14)-3s, which activate T cells and target Trop-2- and CEACAM5-expressing cancer cells, respectively...
August 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28815884/periodontal-ligament-like-tissue-regeneration-with-drilled-porous-decalcified-dentin%C3%A2-matrix-sheet-composite
#6
Ge Feng, Yan Wu, Yu Yong, Lan Huang, Shu An, Bo Hu, Jun Luo, Jinlin Song
OBJECTIVE: In this study, we constructed a composite by combining the human dental follicle cell sheet and a manual drilled porous decalcified dentin matrix that was used to construct ectopic tissue-engineered periodontal ligament-like tissues in renal capsules of nude mice. MATERIALS AND METHODS: Human dental follicle cells were harvested from human lower third molars and then embedded into a temperature-sensitive culture dish. These cells were then placed into frozen porous decalcified dentin matrix sheets and induced by 50 g/ml ascorbic acid...
August 16, 2017: Oral Diseases
https://www.readbyqxmd.com/read/28815591/engineering-defined-membrane-embedded-elements-of-ampa-receptor-induces-opposing-gating-modulation-by-cnih3-and-stargazin
#7
Natalie Hawken, Elena Zaika, Terunaga Nakagawa
During excitatory synaptic transmission, various structurally unrelated transmembrane auxiliary subunits control the function of AMPA receptors (AMPARs), but the underlying mechanisms remain unclear. We identified lipid-exposed residues in the transmembrane domain (TMD) of GluA2 subunit of AMPARs that are critical for the function of AMPAR auxiliary subunits, stargazin (Stg) and cornichon 3 (CNIH3). These residues are essential for stabilizing the AMPAR-CNIH3 complex in detergents and overlap with the contacts made between GluA2 TMD and Stg in the cryoEM structures...
August 17, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28815433/new-insights-on-the-green-synthesis-of-metallic-nanoparticles-using-plant-and-waste-biomaterials-current-knowledge-their-agricultural-and-environmental-applications
#8
Rijuta Ganesh Saratale, Ganesh Dattatraya Saratale, Han Seung Shin, Jaya Mary Jacob, Arivalagan Pugazhendhi, Mukesh Bhaisare, Gopalakrishanan Kumar
Nanotechnology is a rapidly growing scientific field and has attracted a great interest over the last few years because of its abundant applications. Green nanotechnology is a multidisciplinary field that has emerged as a rapidly developing research area, serving as an important technique that emphasize on making the procedure which are clean, non-hazardous, and especially environmentally friendly, in contrast with chemical and physical methods currently employed for nanosynthesis. The biogenic routes could be termed green as these do not involve the use of highly toxic chemicals or elevated energy inputs during the synthesis...
August 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28815272/gold-nanoparticle-based-localized-surface-plasmon-immunosensor-for-staphylococcal-enterotoxin-a-sea-detection
#9
Maroua Ben Haddada, David Hu, Michèle Salmain, Lu Zhang, Chen Peng, Yi Wang, Bo Liedberg, Souhir Boujday
We describe the engineering of stable gold nanoparticle (AuNP) bioconjugates for the detection of staphylococcal enterotoxin A (SEA) using localized surface plasmon resonance (LSPR). Two types of AuNP bioconjugates were prepared by covalently attaching anti-SEA antibody (Ab) or SEA to AuNPs. This was achieved by reacting Traut's reagent with lysine residues of both proteins to generate thiol groups that bind to gold atoms on the AuNP surface. These bioconjugates were characterized in-depth by absorption spectroscopy, cryo-transmission electron microscopy, dynamic light scattering, and zeta potential measurements...
August 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28814761/transplantation-of-bioengineered-rat-lungs-recellularized-with-endothelial-and-adipose-derived-stromal-cells
#10
Ryoichiro Doi, Tomoshi Tsuchiya, Norisato Mitsutake, Satoshi Nishimura, Mutsumi Matsuu-Matsuyama, Yuka Nakazawa, Tomoo Ogi, Sadanori Akita, Hiroshi Yukawa, Yoshinobu Baba, Naoya Yamasaki, Keitaro Matsumoto, Takuro Miyazaki, Ryotaro Kamohara, Go Hatachi, Hideyori Sengyoku, Hironosuke Watanabe, Tomohiro Obata, Laura E Niklason, Takeshi Nagayasu
Bioengineered lungs consisting of a decellularized lung scaffold that is repopulated with a patient's own cells could provide desperately needed donor organs in the future. This approach has been tested in rats, and has been partially explored in porcine and human lungs. However, existing bioengineered lungs are fragile, in part because of their immature vascular structure. Herein, we report the application of adipose-derived stem/stromal cells (ASCs) for engineering the pulmonary vasculature in a decellularized rat lung scaffold...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28813681/uncoupling-the-mitogenic-and-metabolic-functions-of-fgf1-by-tuning-fgf1-fgf-receptor-dimer-stability
#11
Zhifeng Huang, Yi Tan, Junlian Gu, Yang Liu, Lintao Song, Jianlou Niu, Longwei Zhao, Lakshmi Srinivasan, Qian Lin, Jingjing Deng, Yang Li, Daniel J Conklin, Thomas A Neubert, Lu Cai, Xiaokun Li, Moosa Mohammadi
The recent discovery of metabolic roles for fibroblast growth factor 1 (FGF1) in glucose homeostasis has expanded the functions of this classically known mitogen. To dissect the molecular basis for this functional pleiotropy, we engineered an FGF1 partial agonist carrying triple mutations (FGF1(ΔHBS)) that diminished its ability to induce heparan sulfate (HS)-assisted FGF receptor (FGFR) dimerization and activation. FGF1(ΔHBS) exhibited a severely reduced proliferative potential, while preserving the full metabolic activity of wild-type FGF1 in vitro and in vivo...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28812352/ca-%C3%AE-sialon-eu-phosphors-oxidation-states-energy-transfer-and-emission-enhancement-by-incorporation-aimed-surface-engineering
#12
Xiangyang Chen, Lin Gan, Zhi-Jun Zhang, Ping Lu, Xionghui Zeng, Linlin Zhang, Heliang Yao, FangFang Xu, Jing-Tai Zhao
Ca-α-SiAlON: Eu is one of the most promising phosphors exhibiting potential applications in high power LEDs owing to its excellent thermal stability and relative high luminescence efficiency comparable to YAG: Ce commercial phosphors. The oxidation states of Eu ions in Ca-α-SiAlON: Eu are still questionable and furthermore it yet exhibits lower luminescence intensity than commercial yellow phosphors. Therefore, in the present work, the valances of Eu ions in Ca-α-SiAlON have been examined which observed a mixture of divalence and trivalence...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28811868/selaginella-bryopteris-aqueous-extract-improves-stability-and-function-of-cryopreserved-human-mesenchymal-stem-cells
#13
Abhishek Kumar Singh, Anubhuti Jha, Arindam Bit, Andrey P Kiassov, Albert A Rizvanov, Archit Ojha, Pooja Bhoi, Pradeep Kumar Patra, Awanish Kumar, Akalabya Bissoyi
The effective long-term cryopreservation of human mesenchymal stem cells (MSCs) is an essential prerequisite step and represents a critical approach for their sustained supply in basic research, regenerative medicine, and tissue engineering applications. Therefore, attempts have been made in the present investigation to formulate a freezing solution consisting of a combination of Selaginella bryopteris water-soluble extract with and without dimethyl sulfoxide (Me2SO) for the efficient long-term storage of human umbilical cord blood- (hUCB-) derived MSCs...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28811118/prediction-and-characterization-of-the-stability-enhancing-effect-of-the-cherry-tag%C3%A2-in-highly-concentrated-protein-solutions-by-complex-rheological-measurements-and-md-simulations
#14
Pascal Baumann, Marie-Therese Schermeyer, Hannah Burghardt, Cathrin Dürr, Jonas Gärtner, Jürgen Hubbuch
Solution stability attributes are one of the key parameters within the production and launching phase of new biopharmaceuticals. Instabilities of active biological compounds can reduce the yield of biopharmaceutical productions, and may induce undesired reactions in patients, such as immunogenic rejections. Protein solution stability thus needs to be engineered and monitored throughout production and storage. In contrast to the gold standard of long-term storage experiments applied in industry, novel experimental and in silico molecular dynamics tools for predicting protein solution stability can be applied within several minutes or hours...
August 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28811070/evaluation-of-sequence-variability-in-hiv-1-gp41-c-peptide-helix-grafted-proteins
#15
Rachel L Tennyson, Susanne N Walker, Terumasa Ikeda, Reuben S Harris, Brian R McNaughton
Many therapeutically-relevant protein-protein interactions (PPIs) have been reported that feature a helix and helix-binding cleft at the interface. Given this, different approaches to disrupting such PPIs have been developed. While short peptides (<15 amino acids) typically do not fold into a stable helix, researchers have reported chemical approaches to constraining helix structure. However, these approaches rely on laborious, and often expensive, chemical synthesis and purification. Our premise is that protein-based solutions that stabilize a therapeutically-relevant helix offer a number of advantages...
August 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28809500/mechanisms-and-rates-of-u-vi-reduction-by-fe-ii-in-homogeneous-aqueous-solution-and-the-role-of-u-v-disproportionation
#16
Richard N Collins, Kevin M Rosso
Molecular-level pathways in the aqueous redox transformation of uranium by iron remain unclear, despite the importance of this knowledge for predicting uranium transport and distribution in natural and engineered environments. As the relative importance of homogeneous versus heterogeneous pathways is difficult to probe experimentally, here we apply computational molecular simulation to isolate rates of key one electron transfer reactions in the homogenous pathway. By comparison to experimental observations the role of the heterogeneous pathway also becomes clear...
August 15, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28809106/structural-characteristics-and-function-of-a-new-kind-of-thermostable-trehalose-synthase-from-thermobaculum-terrenum
#17
Junqing Wang, Xudong Ren, Ruiming Wang, Jing Su, Feng Wang
Trehalose has important applications in the food industry and pharmaceutical manufacturing. The thermostable enzyme trehalose synthase from Thermobaculum terrenum (TtTS) catalyzes the reversible interconversion of maltose and trehalose. Here, we investigated the structural characteristics of TtTS in complex with the inhibitor TriS. TtTS exhibits the typical three domain glycoside hydrolase family 13 structure. The catalytic cleft consists of Asp202-Glu244-Asp310 and various conserved substrate-binding residues...
August 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28809098/interfacially-engineered-pyridinium-pseudo-gemini-surfactants-as-versatile-and-efficient-supramolecular-delivery-systems-for-dna-sirna-and-mrna
#18
Uttam Satyal, Bogdan Draghici, Lisa L Dragic, Qiangnan Zhang, Kyle Norris, Muniswamy Madesh, Eugen Brailoiu, Marc A Ilies
The article presents the synthesis, self-assembling and biological activity as transfection agents for pDNA, siRNA and mRNA of novel pyridinium pseudo-gemini surfactants, interfacially-engineered from the most efficient gemini surfactants and lipids generated in our amphiphile research program. Formulation of novel amphiphiles in water revealed supramolecular properties very similar to gemini surfactants, despite their lipid-like charge/mass ratio. This dual character was found to also enhance endosomal escape and significantly increase the transfection efficiency...
August 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28807566/electrospun-collagen-based-nanofibres-a-sustainable-material-for-improved-antibiotic-utilisation-in-tissue-engineering-applications
#19
Ivan J Hall Barrientos, Eleonora Paladino, Peter Szabó, Sarah Brozio, Peter J Hall, Charles I Oseghale, Melissa K Passarelli, Susan J Moug, Richard A Black, Clive G Wilson, Romana Zelkó, Dimitrios A Lamprou
For the creation of scaffolds in tissue engineering applications, it is essential to control the physical morphology of fibres and to choose compositions which do not disturb normal physiological function. Collagen, the most abundant protein in the human body, is a well-established biopolymer used in electrospinning compositions. It shows high in-vivo stability and is able to maintain a high biomechanical strength over time. In this study, the effects of collagen type I in polylactic acid-drug electrospun scaffolds for tissue engineering applications are examined...
August 11, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28807154/total-internal-reflectance-fluorescence-imaging-of-genetically-engineered-ryanodine-receptor-targeted-ca-2-probes-in-rat-ventricular-myocytes
#20
Sara Pahlavan, Marin Morad
The details of cardiac Ca(2+) signaling within the dyadic junction remain unclear because of limitations in rapid spatial imaging techniques, and availability of Ca(2+) probes localized to dyadic junctions. To critically monitor ryanodine receptors' (RyR2) Ca(2+) nano-domains, we combined the use of genetically engineered RyR2-targeted pericam probes, (FKBP-YCaMP, Kd=150nM, or FKBP-GCaMP6, Kd=240nM) with rapid total internal reflectance fluorescence (TIRF) microscopy (resolution, ∼80nm). The punctate z-line patterns of FKBP,(2)-targeted probes overlapped those of RyR2 antibodies and sharply contrasted to the images of probes targeted to sarcoplasmic reticulum (SERCA2a/PLB), or cytosolic Fluo-4 images...
September 2017: Cell Calcium
keyword
keyword
95404
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"