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https://www.readbyqxmd.com/read/29715557/native-and-recombinant-phospholipases-a-2-of-scorpio-maurus-venom-glands-impair-angiogenesis-by-targeting-integrins-%C3%AE-5%C3%AE-1-and-%C3%AE-v%C3%AE-3
#1
Najeh Krayem, Zaineb Abdelkefi-Koubaa, Naziha Marrakchi, Youssef Gargouri, José Luis
We recently purified an heterodimeric phospholipase A2 named Sm-PLGV from the venom glands of scorpion Scorpio maurus containing a Long chain, a penta-peptide insertion, which is cut out during the maturation, followed by a short chain. Three recombinant forms of Sm-PLGV were produced in Escherichia coli: rPLA2 (+5) containing the full-length sequence including the penta-peptide insert, rPLA2 (-5) a fused continuous chain of the Long and the short chains without the penta-peptide and the Long chain alone without the short one...
April 28, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29686681/-anaplasma-phagocytophilum-related-defects-in-cd8-nkt-and-nk-lymphocyte-cytotoxicity
#2
Diana G Scorpio, Kyoung-Seong Choi, J Stephen Dumler
Human granulocytic anaplasmosis, caused by the tick-transmitted Anaplasma phagocytophilum , is not controlled by innate immunity, and induces a proinflammatory disease state with innate immune cell activation. In A. phagocytophilum murine infection models, hepatic injury occurs with production of IFNγ thought to be derived from NK, NKT cells, and CD8 T lymphocytes. Specific A. phagocytophilum ligands that drive inflammation and disease are not known, but suggest a clinical and pathophysiologic basis strikingly like macrophage activation syndrome (MAS) and hemophagocytic syndrome (HPS)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29548775/integrin-mediated-human-glioblastoma-cells-adhesion-migration-and-invasion-by-native-and-recombinant-phospholipases-of-scorpio-maurus-venom-glands
#3
Najeh Krayem, Zaineb Abdelkefi-Koubaa, Youssef Gargouri, José Luis
Integrins are a large family of cell surface receptors mediating the interaction of cells with their microenvironment and they play an important role in glioma biology. In the present work, we reported the anti-tumor effect of Sm-PLGV a phospholipase A2 from Tunisian scorpion venom glands-as well as its recombinant forms expressed in Escherichia coli-through interference with integrin receptor function in malignant glioma cells U87. These phospholipases inhibited in a dose dependent manner the adhesion, migration and invasion onto fibrinogen and fibronectin without any cytotoxicity...
May 1, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29540455/improvement-of-bartonella-henselae-dna-detection-in-cat-blood-samples-by-combining-molecular-and-culture-methods
#4
Marina Rovani Drummond, Bruno Grosselli Lania, Pedro Paulo Vissotto de Paiva Diniz, Rovilson Gilioli, Daniele Masselli Rodrigues Demolin, Diana Gerardi Scorpio, Edward B Breitschwerdt, Paulo Eduardo Neves Ferreira Velho
Bartonella spp. are bacteria of worldwide distribution that cause asymptomatic to fatal infections in animals and humans. The most common zoonotic species is Bartonella henselae , for which cats are the major natural reservoir host. To better understand Bartonella sp. diagnostic limitations, we determined the frequency of bloodstream infection in 112 cats by comparing and combining the results of multiple conventional and nested PCRs from blood and liquid culture samples. Using liquid culture conventional PCR, Bartonella sp...
May 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29527829/determination-of-nursing-students-attitudes-towards-the-use-of-technology
#5
Nurten Terkes, Ferya Celik, Hicran Bektas
AIM: The use of technology is increasingly important in nursing education and practice. For this reason, it is necessary to determine the attitudes of nursing students towards technology. METHODS: This study was conducted with 508 nursing students. A personal information form that was prepared by the researchers and the Attitudes Toward Technology Scale were used as the data collection tools. RESULTS: The mean score that was obtained by the nursing students from the Attitudes Toward Technology Scale was 61...
March 11, 2018: Japan Journal of Nursing Science: JJNS
https://www.readbyqxmd.com/read/29486161/anti-angiogenic-effect-of-phospholipases-a-2-from-scorpio-maurus-venom-glands-on-human-umbilical-vein-endothelial-cells
#6
Najeh Krayem, Zaineb Abdelkefi-Koubaa, Naziha Marrakchi, José Luis, Youssef Gargouri
In a previous study, we purified Sm-PLGV an heterodimeric phospholipase A2 , from the venom glands of the Tunisian scorpion Scorpio maurus. This enzyme contains a Long chain, a penta-peptide insertion, which is cut out during the maturation process, followed by a short chain. A disulfide bridge links the two chains. Three recombinant forms of this enzyme were produced in Escherichia coli: rPLA2 (+5) with a penta-peptide insert, rPLA2 (-5) without the penta-peptide, and the Long chain alone without the short one...
April 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29477749/functional-characterization-and-ftir-based-3d-modeling-of-full-length-and-truncated-forms-of-scorpio-maurus-venom-phospholipase-a-2
#7
Najeh Krayem, Goetz Parsiegla, Hélène Gaussier, Hanen Louati, Raida Jallouli, Pascal Mansuelle, Frédéric Carrière, Youssef Gargouri
BACKGROUND: Heterodimeric phospholipase A2 from venom glands of Tunisian scorpion Scorpio maurus (Sm-PLGV) had been purified. It contains long and short chains linked by a disulfide bridge. Sm-PLGV exhibits hemolytic activity towards human erythrocytes and interacts with phospholipid monolayers at high surface pressure. The investigation of structure-function relationships should provide new clues to understand its activity. METHODS: Molecular cloning of Sm-PLGV and heterologous expression in Escherichia coli of three recombinant forms was used to determine the role of the short chain on enzymatic activity...
May 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29444432/surface-matrix-screening-identifies-semi-specific-interactions-that-improve-potency-of-a-near-pan-reactive-hiv-1-neutralizing-antibody
#8
Young D Kwon, Gwo-Yu Chuang, Baoshan Zhang, Robert T Bailer, Nicole A Doria-Rose, Tatyana S Gindin, Bob Lin, Mark K Louder, Krisha McKee, Sijy O'Dell, Amarendra Pegu, Stephen D Schmidt, Mangaiarkarasi Asokan, Xuejun Chen, Misook Choe, Ivelin S Georgiev, Vivian Jin, Marie Pancera, Reda Rawi, Keyun Wang, Rajoshi Chaudhuri, Lisa A Kueltzo, Slobodanka D Manceva, John-Paul Todd, Diana G Scorpio, Mikyung Kim, Ellis L Reinherz, Kshitij Wagh, Bette M Korber, Mark Connors, Lawrence Shapiro, John R Mascola, Peter D Kwong
Highly effective HIV-1-neutralizing antibodies could have utility in the prevention or treatment of HIV-1 infection. To improve the potency of 10E8, an antibody capable of near pan-HIV-1 neutralization, we engineered 10E8-surface mutants and screened for improved neutralization. Variants with the largest functional enhancements involved the addition of hydrophobic or positively charged residues, which were positioned to interact with viral membrane lipids or viral glycan-sialic acids, respectively. In both cases, the site of improvement was spatially separated from the region of antibody mediating molecular contact with the protein component of the antigen, thereby improving peripheral semi-specific interactions while maintaining unmodified dominant contacts responsible for broad recognition...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29322054/spatial-distribution-of-medically-important-scorpions-in-north-west-of-iran
#9
Mulood Mohammadi Bavani, Javad Rafinejad, Ahmad Ali Hanafi-Bojd, Mohammad Ali Oshaghi, Shahrokh Navidpour, Farrokh Dabiri, Mehdi Badakhshan, Esmaeil Ghorbani, Masoomeh Bagheri
Background: Scorpions are one of the most important medical arthropods in Iran. This study aimed to determine the fauna, spatial distribution and some morphological characteristics of these venomous arthropods in the study area. Methods: Scorpions were collected using Ultra Violet light, rock rolling and digging methods in West Azerbaijan, East Azerbaijan, and Ardabil Provinces during 2015-2016. The specimens were preserved in 75% ethyl alcohol and transferred to the laboratory for species identification and morphological studies...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29274859/variable-tibiofemoral-articular-contact-stress-in-fixed-bearing-total-knee-arthroplasties
#10
K Murakami, S Hamai, T Moro-Oka, K Okazaki, H Higaki, T Shimoto, S Ikebe, Y Nakashima
BACKGROUND: Rotational allowance at the tibiofemoral joint would be required during deep flexion. However, the amount of flexion and rotation has not been investigated in modern total knee arthroplasty (TKA) designs. The present study aimed to determine the contact stress in five posterior-stabilized fixed-bearing TKA designs. HYPOTHESIS: We hypothesized that the contact area and stresses at the tibiofemoral articular surfaces vary according to the type of implant design and tested condition...
April 2018: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/29197122/efficacy-of-minocycline-in-naturally-occurring-nonacute-ehrlichia-canis-infection-in-dogs
#11
S Jenkins, J K Ketzis, J Dundas, D Scorpio
BACKGROUND: Minocycline has been used in the treatment of Ehrlichia canis infection in dogs as an alternative to doxycycline, the recommended treatment. However, efficacy of this alternative therapy is unknown. OBJECTIVE: To assess the efficacy of minocycline in the treatment of natural occurring E. canis infection in dogs. ANIMALS: Ten privately owned dogs of mixed breed positive for E. canis by blood PCR. METHODS: Prospective, randomized clinical study...
January 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29062849/fauna-and-geographical-distribution-of-scorpions-in-ilam-province-south-western-iran
#12
Narges Sharifinia, Iman Gowhari, Manijeh Hoseiny-Rad, Ali Ashraf Aivazi
BACKGROUND: Scorpions' stings and their own mortalities place them among the most important health and medical problems. The dreadful features and especially their poisonous stings are considered a major cause of human stress and abhorrence/phobia. The current study aimed to study the scorpion fauna of Ilam Province, south western Iran in order to manage scorpionism related problems. METHODS: In this field-laboratory investigation during March 2014 to February 2015, different parts of Ilam Province were surveyed...
June 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29026854/a-comparative-study-of-nesting-sites-and-burrowing-habits-of-two-iranian-burrowing-scorpions
#13
Babak Vazirianzadeh, Amir Jalali, Mostafa Chrom, Alireza Mohammady, Hassan Vatandoost, Forozan Panahi
BACKGROUND: The different features of scorpions can be successfully described by their nesting and burrowing behaviors. There is little information about burrowing activity of Iranian scorpions. METHODS: The current study was performed to compare the burrowing behavior between two burrowing Iranian scorpions, Scorpio maurus and Odonthubutus bidentatus by describing 30 nests of each species regarding collecting the scorpions. RESULTS: Scorpio maurus and O...
March 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/28616470/global-solutions-to-regional-challenges-bridging-the-one-health-divide-in-the-caribbean
#14
Arve Lee Willingham, Luis Cruz-Martinez, Diana G Scorpio, Christa A Gallagher
Ross University School of Veterinary Medicine, located on the Caribbean island of St. Kitts in the West Indies, hosted a multi-national, transdisciplinary One Health conference in St. Kitts and Nevis. Historically the many countries of the Caribbean carry a high burden of chronic and infectious disease and struggle with complex economic and developmental issues that continuously pressurize inhabitants and their natural environment. Considering these vast regional challenges, presentations covered diverse topics including community-based approaches for zoonotic disease control and prevention and mitigation of problems at the interface of wildlife, domestic animals, and humans...
December 2016: One Health
https://www.readbyqxmd.com/read/28515812/cost-effectiveness-of-mirabegron-compared-to-tolterodine-er-4-mg-for-overactive-bladder-in-canada
#15
Sender Herschorn, Jameel Nazir, Barbara Ramos, Zalmai Hakimi
INTRODUCTION: This analysis compared the cost-effectiveness of once-daily regimens of mirabegron 50 mg and generic tolterodine ER 4 mg in a hypothetical cohort of previously treated patients with overactive bladder (OAB) in Canada. METHODS: A Markov model was developed to represent different health states according to OAB symptoms (frequency, incontinence), presence/absence of adverse events (AEs; dry mouth, constipation, blurred vision), and treatment status (on-treatment, discontinue treatment, restart previous treatment)...
March 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/28476062/patient-reported-outcomes-following-single-and-multiple-radius-total-knee-replacement-a-randomized-controlled-trial
#16
Nadeem Mushtaq, Alexander D Liddle, David Isaac, Katherine Dillow, Paul Gill
Although single-radius (SR) designs of total knee replacement (TKR) have theoretical benefits, the clinical advantage conferred by such designs is unknown. The aim of this randomized, controlled study was to compare the short-term clinical outcomes of the two design rationales. A total of 105 knees were randomized to receive either a single radius (Scorpio, Stryker; SR Group) or multiple radius (AGC, Zimmer Biomet; MR group) TKR. Patient-reported outcomes (Oxford Knee Score [OKS] and Knee Society Score [KSS]) were collected at 6 weeks, 6 months, and 1 year following surgery...
January 2018: Journal of Knee Surgery
https://www.readbyqxmd.com/read/28445724/quantification-of-the-impact-of-the-hiv-1-glycan-shield-on-antibody-elicitation
#17
Tongqing Zhou, Nicole A Doria-Rose, Cheng Cheng, Guillaume B E Stewart-Jones, Gwo-Yu Chuang, Michael Chambers, Aliaksandr Druz, Hui Geng, Krisha McKee, Young Do Kwon, Sijy O'Dell, Mallika Sastry, Stephen D Schmidt, Kai Xu, Lei Chen, Rita E Chen, Mark K Louder, Marie Pancera, Timothy G Wanninger, Baoshan Zhang, Anqi Zheng, S Katie Farney, Kathryn E Foulds, Ivelin S Georgiev, M Gordon Joyce, Thomas Lemmin, Sandeep Narpala, Reda Rawi, Cinque Soto, John-Paul Todd, Chen-Hsiang Shen, Yaroslav Tsybovsky, Yongping Yang, Peng Zhao, Barton F Haynes, Leonidas Stamatatos, Michael Tiemeyer, Lance Wells, Diana G Scorpio, Lawrence Shapiro, Adrian B McDermott, John R Mascola, Peter D Kwong
While the HIV-1-glycan shield is known to shelter Env from the humoral immune response, its quantitative impact on antibody elicitation has been unclear. Here, we use targeted deglycosylation to measure the impact of the glycan shield on elicitation of antibodies against the CD4 supersite. We engineered diverse Env trimers with select glycans removed proximal to the CD4 supersite, characterized their structures and glycosylation, and immunized guinea pigs and rhesus macaques. Immunizations yielded little neutralization against wild-type viruses but potent CD4-supersite neutralization (titers 1: >1,000,000 against four-glycan-deleted autologous viruses with over 90% breadth against four-glycan-deleted heterologous strains exhibiting tier 2 neutralization character)...
April 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28364072/do-cats-serve-as-good-sentinels-for-bartonella-species-infection-risk-in-people-and-animals
#18
EDITORIAL
Diana G Scorpio
No abstract text is available yet for this article.
April 1, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28208885/study-on-distribution-of-scorpions-to-provide-prevention-and-interventions-in-combating-scorpionism-in-poldokhtar-county-lorestan-province-iran
#19
Mansour Nazari, Rastgar Hassan
INTRODUCTION: Scorpions are arthropods of medical importance classified in the class Arachnida, inhabiting hot and dry environments. All scorpions have a venomous sting and several thousand people die each year from scorpion stings, but this mortality is due to the venom of about 25 species located in northern Africa, the Middle East, India, Mexico and parts of South America. Poldokhtar County belongs to one of the southern cities of Lorestan Province, providing suitable habitats for many different species of scorpions due to its specific climatic conditions...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27932550/spermaurin-an-la1-like-peptide-from-the-venom-of-the-scorpion-scorpio-maurus-palmatus-improves-sperm-motility-and-fertilization-in-different-mammalian-species
#20
Guillaume Martinez, Jean-Pascal Hograindleur, Sébastien Voisin, Roland Abi Nahed, Tarek M Abd El Aziz, Jessica Escoffier, Julien Bessonnat, Claire-Maëlle Fovet, Michel De Waard, Sylviane Hennebicq, Vincent Aucagne, Pierre F Ray, Eric Schmitt, Philippe Bulet, Christophe Arnoult
STUDY QUESTION: Is it possible to identify original compounds that are able to enhance sperm motility from the venom of the scorpion Scorpio maurus palmatus? SUMMARY ANSWER: We identified a potent disulfide-rich peptide (DRP) of 73 amino acids that significantly improved the motility of fresh and frozen-thawed sperm in different mammalian species, including human, and improved fertilization outcome in mouse IVF experiments. WHAT IS KNOWN ALREADY: Any disturbance of sperm motility has a strong impact on fertilization and can lead to subfertility or infertility...
February 10, 2017: Molecular Human Reproduction
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