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https://www.readbyqxmd.com/read/29596851/gills-specific-type-2-crustin-isoforms-its-molecular-cloning-and-characterization-from-kuruma-shrimp-marsupenaeus-japonicus
#1
Gauravkumar M Tandel, Hidehiro Kondo, Ikuo Hirono
Crustins are diverse group of antimicrobial peptides (AMPs) which have numerous isoforms mainly identified from hemocytes in decapods crustacean. However, little is known about its presence solely in gills tissue. In this study, we found two new crustin isoforms MjCRS8 and MjCRS9 by using transcriptome analysis from gills. Open reading frame of MjCRS8 and MjCRS9 were 593 bp and 459 bp encoding 197aa and 152aa, respectively. Tissue distribution analysis indicated that both MjCRS8 and MjCRS9 are expressed only in gills tissue...
March 26, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29471061/effects-of-dietary-inulin-and-mannan-oligosaccharide-on-immune-related-genes-expression-and-disease-resistance-of-pacific-white-shrimp-litopenaeus-vannamei
#2
Yun Li, Hong Liu, Xilin Dai, Jingjing Li, Fujiang Ding
The effects of inulin and mannan oligosaccharide (MOS) at different doses (2.5, 4 and 10 mg/g) in singular or combined diet on growth rate, immune related genes expression, and resistance to white spot syndrome virus (WSSV) and Vibrio alginolyticus in Pacific white shrimp (Litopenaeus vannamei) were investigated. At the end of 28-day singular feeding experiment, the highest values of specific growth rate (SGR) and the expression of toll-like receptor1, 2 and 3 (TLR1, 2, 3), signal transducer and activator of transcription (STAT), crustin, anti-lipopolysaccharide factor (ALF) as well as prophenoloxidase (proPO) were observed in shrimp individually fed with 5 mg/g dietary inulin or MOS, respectively...
February 19, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29462747/involvement-of-a-newly-identified-atypical-type-ii-crustin-spcrus5-in-the-antibacterial-immunity-of-mud-crab-scylla-paramamosain
#3
Yue Wang, Chao Zhang, Hui Wang, Hongyu Ma, Yan-Qing Huang, Jian-Xue Lu, Xin-Cang Li, Xiao-Wen Zhang
Crustins, the main AMP family in Crustacea, are generated as isoforms in many species and implicated in innate immune responses, but their detailed molecular mechanisms on susceptible bacteria remain largely unclear. Type II and type I crustins are distinguished by glycine-rich region (GRR), which is a major marker motif, and some type II crustins exhibit stronger antibacterial activities than their GRR deletion mutants. In the present study, a novel crustin, namely, SpCrus5, was functionally characterized from a commercially valuable crab Scylla paramamosain...
February 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29409789/newly-identified-type-ii-crustin-spcrus2-in-scylla-paramamosain-contains-a-distinct-cysteine-distribution-pattern-exhibiting-broad-antimicrobial-activity
#4
Hui Wang, Jing-Xiao Zhang, Yue Wang, Wen-Hong Fang, Yuan Wang, Jun-Fang Zhou, Shu Zhao, Xin-Cang Li
Type II crustins are the most abundant type of crustins in shrimps that exhibit remarkable sequence diversities and broad antibacterial activities. This study characterized a novel type II crustin, SpCrus2, in the mud crab Scylla paramamosain. The SpCrus2 cDNA sequence is 620-bp long with a 495-bp open reading frame encoding a 164-amino acid protein. In the deduced protein, a 17-amino acid signal peptide, a glycine-rich hydrophobic region (GRR), and a cysteine-rich region (CRR) containing a whey acidic protein domain were predicted...
February 2, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29407617/marine-actinomycetes-as-bioremediators-in-penaeus-monodon-rearing-system
#5
Divya T Babu, K Archana, Bhavya Kachiprath, Solly Solomon, G Jayanath, I S Bright Singh, Rosamma Philip
Actinomycetes (277Nos) isolated from marine environments and shrimp culture pond sediments were tested for hydrolytic enzyme production and biogranulation property. Potential isolates were screened for their efficacy in bioremediation of shrimp culture system. Based on the BOD reduction efficiency and water quality parameters, five actinomycete isolates viz., Streptomyces coelicoflavus (A6), Streptomyces diastaticus (A 44), Nocardiopsis alba (A 55), Streptomyces parvus (A 56) and Streptomyces champavatii (R 32) were subjected for tertiary screening in Penaeus monodon larval rearing system and the animals were challenged with white spot syndrome virus (WSSV)...
January 30, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29352984/spcrus3-and-spcrus4-share-high-similarity-in-mud-crab-scylla-paramamosain-exhibiting-different-antibacterial-activities
#6
Yue Wang, Xiao-Wen Zhang, Hui Wang, Wen-Hong Fang, Hongyu Ma, Fengxia Zhang, Yuan Wang, Xin-Cang Li
Type I crustins are crucial effectors of crustacean immune system. Various type I crustins with high sequence diversity possess different antimicrobial activities. To date, the mechanism on how the sequence diversity of type I crustins affects their antimicrobial activities is largely unclear, and how different crustins function together against bacterial invasion still remains unknown. In this study, we identified two novel type I crustins, namely, SpCrus3 and SpCrus4, from an economically important crab, Scylla paramamosain...
May 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29337853/specific-molecular-signatures-for-type-ii-crustins-in-penaeid-shrimp-uncovered-by-the-identification-of-crustin-like-antimicrobial-peptides-in-litopenaeus-vannamei
#7
Cairé Barreto, Jaqueline da Rosa Coelho, Jianbo Yuan, Jianhai Xiang, Luciane Maria Perazzolo, Rafael Diego Rosa
Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or "Crustins" and Type IIb or "Crustin-like") possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in penaeid shrimp. To gain insight into the molecular diversity of Type II crustins in penaeids, we identified and characterized a Type IIb crustin in Litopenaeus vannamei (Crustin-like Lv ) and compared Type II crustins at both molecular and transcriptional levels...
January 16, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29288814/immune-response-of-exopalaemon-carinicauda-infected-with-an-ahpnd-causing-strain-of-vibrio-parahaemolyticus
#8
Qianqian Ge, Jian Li, Jitao Li, Jiajia Wang, Zhengdao Li
To investigate the immune response of Exopalaemon carinicauda infected with an AHPND-causing strain of Vibrio parahaemolyticus (VPAHPND ), three-generation breeding of shrimp selected for their survival to VPAHPND infection was applied to explore the relationship between immune parameters and AHPND-resistant capacity of E. carinicauda. In this study, the LD50 dose of 48 h and survival rates at 144 h of shrimp to VPAHPND increased from 106.0 to 106.6  cfu ml-1 and from 26.67% to 36.67% by three successive generations selection, respectively, while there was no significant difference between the first and second generation (p > ...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29288050/enhancement-of-immune-response-and-resistance-against-vibrio-parahaemolyticus-in-kuruma-shrimp-marsupenaeus-japonicus-by-dietary-supplementation-of-%C3%AE-1-4-mannobiose
#9
Gehad E Elshopakey, Engy F Risha, Osama A Abdalla, Yo Okamura, Vu Duc Hanh, Masahisa Ibuki, Raja Sudhakaran, Toshiaki Itami
Supplementation of prebiotic carbohydrates can act as a potent immunomodulator and have the efficacy to induce immune-related genes which are involved in host defense. Pure β-1,4-mannobiose (MNB) showed activation of prophenoloxidase system of shrimp hemocytes in vitro. The resistance of kuruma shrimp Marsupenaeus japonicus against Vibrio parahaemolyticus was examined after the shrimp were fed with 0 (control), 0.02, 0.2, and 2% MNB supplemented diets. The results showed significantly higher survival rates in MNB supplemented shrimp than those of the control one from 2 to 12 days post challenge...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29247794/a-single-wap-domain-swd-containing-protein-with-antiviral-activity-from-pacific-white-shrimp-litopenaeus-vannamei
#10
Linwei Yang, Shengwen Niu, Jiefeng Gao, Hongliang Zuo, Jia Yuan, Shaoping Weng, Jianguo He, Xiaopeng Xu
The single whey acidic protein (WAP) domain (SWD)-containing proteins, also called type III crustins, are a group of antimicrobial peptides (AMPs) in crustaceans. At present, a number of SWDs have been identified in shrimp, which showed essential antibacterial activities. However, the roles of SWDs in antiviral immune responses have not been reported up to now. In this study, a novel SWD (LvSWD3) was identified from Pacific white shrimp, Litopenaeus vannamei, which contained a typical single WAP domain homologous to those of other crustacean SWDs...
February 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29208497/molecular-cloning-expression-promoter-analysis-and-functional-characterization-of-a-new-crustin-from-litopenaeus-vannamei
#11
Ming Li, Chunxia Ma, Haoyang Li, Jinxia Peng, Digang Zeng, Xiaohan Chen, Chaozheng Li
Antimicrobial peptides (AMPs) are the most important players in the innate immune system, providing a principal first-line of defense against the invading pathogens. Crustin, a type of whey acidic protein (WAP) domain-containing and cationic cysteine-rich AMP, can function in a protease inhibition or an effector molecule manner. In the present study, a new Crustin was cloned and identified from Pacific white shrimp Litopenaeus vannamei and designated as LvCrustinA. The full-length cDNA of LvCrustinA was 687 bp, with a 519 bp open reading frame (ORF) that encoded a peptide of 172 amino acids...
February 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29054827/characterization-of-a-double-wap-domain-containing-protein-from-the-red-swamp-crayfish-procambarus-clarkii
#12
Hong-Wei Zhang, Xin Man, Yue Wang, Qi-Sheng Song, David Stanley, Kai-Min Hui, Xiao-Wen Zhang
Crustaceans express multiple whey acidic protein (WAP) domain containing proteins which are components of host immunity. In the present study, a new double WAP domain containing protein was identified from red swamp crayfish Procambarus clarkii, designated Pc-DWD. The ORF is 387 bp, encoding 128 amino acids consisting of signal peptide of 18 residues, and two tandem WAP domains of 38 and 44 residues. Multiple alignment indicates the presence of conserved motifs in both WAP domains, and phylogenetic analysis shows that Pc-DWD is a new member of the type-IV crustin family...
October 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29017939/identification-of-a-novel-toll-gene-shtoll3-from-the-freshwater-crab-sinopotamon-henanense-and-its-expression-pattern-changes-in-response-to-cadmium-followed-by-aeromonas-hydrophila-infection
#13
Lang Lang, Zuobing Zhang, Weixin Jing, Jiang-Shiou Hwang, Shao-Chin Lee, Lan Wang
Toll signaling is essential for expression of immune genes which are important for defense against bacterial, fungal and viral infections in invertebrates. Although several toll genes have been identified in the crustaceans, none of them has been investigated in freshwater crab Sinopotamon henanense. Moreover, the effect of cadmium (Cd) on toll gene expression has never been examined on the freshwater crabs which live in the sediments and are prone to heavy metal bioaccumulation. Our transcriptomic analysis of hepatopancreas tissue reveals that toll3 gene expression has been decreased when treated with Cd...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28970971/advances-in-the-study-of-nodavirus
#14
Chean Yeah Yong, Swee Keong Yeap, Abdul Rahman Omar, Wen Siang Tan
Nodaviruses are small bipartite RNA viruses which belong to the family of Nodaviridae. They are categorized into alpha-nodavirus, which infects insects, and beta-nodavirus, which infects fishes. Another distinct group of nodavirus infects shrimps and prawns, which has been proposed to be categorized as gamma-nodavirus. Our current review focuses mainly on recent studies performed on nodaviruses. Nodavirus can be transmitted vertically and horizontally. Recent outbreaks have been reported in China, Indonesia, Singapore and India, affecting the aquaculture industry...
2017: PeerJ
https://www.readbyqxmd.com/read/28964865/molecular-cloning-recombinant-expression-and-functional-characterization-of-an-antimicrobial-peptide-crustin-from-the-indian-white-shrimp-fenneropenaeus-indicus
#15
K S Sruthy, Aishwarya Nair, J Puthumana, Swapna P Antony, I S Bright Singh, Rosamma Philip
Antimicrobial peptides (AMPs) comprise molecules that involve in the defense mechanism of various organisms towards pathogens such as bacteria, fungi, parasites and viruses. Crustins are generally defined as multi-domain cationic antimicrobial peptides containing one whey acidic protein (WAP) domain at the C-terminus as the functional unit. In this study, we identified and characterized a novel crustin homolog (Fi-Crustin2) with 354 bp fragment cDNA encoding 117 amino acids and an ORF of 100 amino acids with a net charge of +1 from the mRNA of F...
September 28, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28935602/dietary-immunogen-%C3%A2-modulated-digestive-enzyme-activity-and-immune-gene-expression-in-litopenaeus-vannamei-post-larvae
#16
Hamed Kolangi Miandare, Ali Taheri Mirghaed, Marjan Hosseini, Nastaran Mazloumi, Ashkan Zargar, Sajad Nazari
Pacific white shrimp Litopenaeus vannamei (Boone, 1931) is an important economical shrimp species worldwide, especially in the Middle East region, and farming activities of this species have been largely affected by diseases, mostly viral and bacterial diseases. Scientists have started to use prebiotics for bolstering the immune status of the animal. This study aimed to investigate the influence of Immunogen(®) on growth, digestive enzyme activity and immune related gene expression of Litopenaeus vannamei post-larvae...
September 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28764985/function-of-two-ficolin-like-proteins-in-innate-immune-defense-of-the-oriental-river-prawn-macrobrachium-nipponense
#17
Tingting Li, Lei Wu, Min Jin, Futong Ma, Xin Huang, Qian Ren
Ficolins have crucial functions in recognizing and eliminating pathogens in innate immunity. In this study, we identified two ficolin-like genes from the oriental river prawn, Macrobrachium nipponense. These genes were designated as MnFico1 and MnFico2. MnFico1 cDNA has 1600 bp, whereas MnFico2 has 1486 bp. In addition to a coiled-coil region or a low complexity region, the two ficolins both contained a signal peptide and a fibrinogen-related domain. qRT-PCR results showed that the highest expression level of MnFico1 expression was in the gills, whereas that of MnFico2 was in the heart...
July 29, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28750060/correction-the-new-face-of-the-old-molecules-crustin-pm4-and-transglutaminase-type-i-serving-as-rnps-down-regulate-astakine-mediated-hematopoiesis
#18
Yun-Tsan Chang, Cheng-Yung Lin, Che-Yiang Tsai, Vinu S Siva, Chia-Ying Chu, Huai-Jen Tsai, Yen-Ling Song
[This corrects the article DOI: 10.1371/journal.pone.0072793.].
2017: PloS One
https://www.readbyqxmd.com/read/28743625/a-single-wap-domain-containing-protein-from-litopenaeus-vannamei-possesses-antiproteinase-activity-against-subtilisin-and-antimicrobial-activity-against-ahpnd-inducing-vibrio-parahaemolyticus
#19
Suwattana Visetnan, Premruethai Supungul, Anchalee Tassanakajon, Suchao Donpudsa, Vichien Rimphanitchayakit
The single WAP domain-containing protein (SWD) is a type III crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. A study of SWD from Litopenaeus vannamei (LvSWD) is reported herein about its activities and function against bacteria, particularly the AHPND-inducing Vibrio parahaemolyticus (VPAHPND) that causes acute hepatopancreatic necrosis disease (AHPND). The LvSWD is mainly synthesized in hemocytes and up-regulated in response to VPAHPND infection...
July 22, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28655578/unusual-tissue-distribution-of-carcinin-an-antibacterial-crustin-in-the-crab-carcinus-maenas-reveals-its-multi-functionality
#20
Suzanne Suleiman, Valerie J Smith, Elisabeth A Dyrynda
Crustins are whey acidic four-disulphide core (WFDSC) domain-containing proteins in decapods that are widely regarded as antimicrobial agents that contribute to host defence. Whilst there have been many analyses of crustin gene expression in tissues, few studies have been made of the distribution of the natural proteins. Here we report an immunostaining investigation of carcinin, a native crustin from Carcinus maenas, in the body organs. The results show that the protein is largely confined to the haemocytes with only a weak signal detected in the heart, hepatopancreas and midgut caecum where it is restricted to the outer surfaces...
June 24, 2017: Developmental and Comparative Immunology
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