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Olga M Koper, Joanna Kamińska, Katarzyna Pańkowska, Anna Matosek, Edyta Suchodoła, Karolina Sprawka, Anna Tenderenda, Paulina Brania, Agnieszka Statkiewicz, Halina Kemona
Disease processes may impair the production and reabsorption of fluid from in the body cavities, which results in its excessive accumulation. AIM: The aim of the study was the evaluation of difficulties regarding the classification of fluids from the body cavities into transudate/exudate observing the following: Light's criteria, total fluid protein concentration, and total protein ratio (TP ratio) and lactate dehydrogenase ratio (LDH ratio). MATERIALS AND METHODS: Retrospective analysis was conducted on pleural (N=314), peritoneal (N=114) and pericardial (N=10) fluids, which were tested for the total protein concentration and LDH activity both in fluid and serum and calculated on TP ratio and LDH ratio...
November 23, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
Penelope J E Quintana, Mehdi Khalighi, Javier Emmanuel Castillo Quiñones, Zalak Patel, Jesus Guerrero Garcia, Paulina Martinez Vergara, Megan Bryden, Antoinette Mantz
At US-Mexico border Ports of Entry, vehicles idle for long times waiting to cross northbound into the US. Long wait times at the border have mainly been studied as an economic issue, however, exposures to emissions from idling vehicles can also present an exposure risk. Here we present the first data on in-vehicle exposures to driver and passengers crossing the US-Mexico border at the San Ysidro, California Port of Entry (SYPOE). Participants were recruited who regularly commuted across the border in either direction and told to drive a scripted route between two border universities, one in the US and one in Mexico...
December 4, 2017: Science of the Total Environment
Paulina Katarzyna Kęszycka, Michał Szkop, Danuta Gajewska
The study aimed to determine the salicylates content in 112 products available on the European market. Quantitative determination of free and conjugated forms of salicylic acid (SA) in food was performed using reversed phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection. The salicylates contents ranged from 0 to 1675.79 (µg/100 g). The results of this study confirm the presence of salicylates in food products, as well as a broad content diversity of these compounds depending on the species, variety and method of processing of food items...
November 28, 2017: Journal of Agricultural and Food Chemistry
Paulina Stehlik, Astrid Frotjold, Carl R Schneider
Interprofessional learning (IPL) during formal training enables interprofessional collaboration (IPC) in the workforce; however, on-campus IPL opportunities are seldom incorporated into curricula. We describe the development and implementation of two hospital simulation tutorials between nursing and pharmacy students. Students were required to provide "usual care" to a simulated patient at admission and discharge. A pre-post survey design was used to evaluate changes in Interdisciplinary Education Perception Scale (IEPS) score and student perceived educational value of the tutorials...
January 2018: Journal of Interprofessional Care
Paulina Hawkins, Erik Mercado, Sopio Chochua, Maria E Castillo, Isabel Reyes, Eduardo Chaparro, Rebecca Gladstone, Stephen D Bentley, Robert F Breiman, Benjamin J Metcalf, Bernard Beall, Theresa J Ochoa, Lesley McGee
Before PCV7 introduction, invasive pneumococcal disease (IPD) was responsible for approximately 12,000-18,000 deaths annually among children <5years in Latin America. In Peru, PCV7 was introduced in 2009. We used whole genome sequencing to deduce key features of invasive strains collected in Lima, Peru from 2006 to 2011. We sequenced 212 IPD isolates from 16 hospitals in Lima pre (2006-2009; n=133) and post (2010-2011; n=79) PCV7 introduction; 130 (61.3%) isolates were from children≤5years old. CDC's Streptococcus lab bioinformatics pipeline revealed serotypes, sequence types (STs), pilus genes, PBP types and other resistance determinants...
October 2017: International Journal of Medical Microbiology: IJMM
Andrea Huneeus, Mario I Fernández, Andrea Schilling, Paulina Parra, Aleksandra Zakharova
BACKGROUND: As alternative for patients that fear genital examination, we assessed adolescent's comfort and ease with self-collected samples for nucleic acid amplification testing for sexually transmitted infections. PATIENTS AND METHODS: Sexually active Chilean adolescents and youth under 25 years (174 males and 117 females) were enrolled. Females used self-collected vaginal swabs and males collected first-stream urine. A satisfaction survey evaluating self-sampling system was applied...
April 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Paulina Jędrak, Magdalena Krygier, Katarzyna Tońska, Małgorzata Drozd, Magdalena Kaliszewska, Ewa Bartnik, Witold Sołtan, Emilia J Sitek, Anna Stanisławska-Sachadyn, Janusz Limon, Jarosław Sławek, Grzegorz Węgrzyn, Sylwia Barańska
Huntington disease (HD) is an inherited neurodegenerative disorder caused by mutations in the huntingtin gene. Involvement of mitochondrial dysfunctions in, and especially influence of the level of mitochondrial DNA (mtDNA) on, development of this disease is unclear. Here, samples of blood from 84 HD patients and 79 controls, and dermal fibroblasts from 10 HD patients and 9 controls were analysed for mtDNA levels. Although the type of mitochondrial haplogroup had no influence on the mtDNA level, and there was no correlation between mtDNA level in leukocytes in HD patients and various parameters of HD severity, some considerable differences between HD patients and controls were identified...
August 2017: Metabolic Brain Disease
Paulina Yesica Ochoa-Martínez, Javier Arturo Hall-López, Marco Antonio Martínez-García, Raúl Díaz-Molina, Ana María Miranda Botelho Teixeira, José Antonio Moncada-Jiménez
BACKGROUND: Aim: To compare the concentration of secretory immunoglobulin-A (sIgA) in young adults with average or excellent aerobic capacity before and after a cardiopulmonary graded exercise test. METHODS: Participants were nine apparently healthy physically active males (Mean age = 21.3 ± 2.1 yr.), randomly allocated in two groups based on their VO2max: a) average aerobic capacity (AEC, n = 5) or b) excellent aerobic capacity (EAC, n = 4). Participants performed the Bruce protocol to determine their aerobic capacity...
February 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Agnieszka Horala, Agata Swiatly, Jan Matysiak, Paulina Banach, Ewa Nowak-Markwitz, Zenon J Kokot
As cancer development involves pathological vessel formation, 16 angiogenesis markers were evaluated as potential ovarian cancer (OC) biomarkers. Blood samples collected from 172 patients were divided based on histopathological result: OC (n = 38), borderline ovarian tumours (n = 6), non-malignant ovarian tumours (n = 62), healthy controls (n = 50) and 16 patients were excluded. Sixteen angiogenesis markers were measured using BioPlex Pro Human Cancer Biomarker Panel 1 immunoassay. Additionally, concentrations of cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) were measured in patients with adnexal masses using electrochemiluminescence immunoassay...
January 10, 2017: International Journal of Molecular Sciences
Paulina Przyborowska, Zbigniew Adamiak, Yauheni Zhalniarovich
Objectives The aim of this study was to evaluate variations in lateral ventricles in the examined feline population with the use of quantitative analysis methods to determine whether sex or body weight influenced the size of the ventricles, and to identify any significant differences in the results of low- and high-field MRI. Methods Twenty healthy European Shorthair cats, aged 1-3 years, with body weights ranging from 2.85-4.35 kg, were studied. MRI of brain structures was performed in a low- and a high-field MRI system...
October 2017: Journal of Feline Medicine and Surgery
Diana Yessenia Calva Delgado, Zorayda Patricia Toledo Barrigas, Sofía Genoveva Ochoa Astutillo, Ana Paulina Arévalo Jaramillo, Alessio Ausili
This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of β-lactamases and ESBLs with blaTEM and blaCTX-M as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different β-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 β-lactamase and the presence of different CTX-M variants with a prevalence of CTX-M-15...
November 2016: Brazilian Journal of Infectious Diseases
Adam Finn, Begonia Morales-Aza, Paulina Sikora, Jessica Giles, Ryan Lethem, Matko Marlais, Valtyr Thors, Andrew J Pollard, Saul Faust, Paul Heath, Ian Vipond, Muriel Ferreira, Peter Muir, Luís Januário, Fernanda Rodrigues
BACKGROUND: Improved understanding of Neisseria meningitidis (Nm) carriage biology and better methods for detection and quantification would facilitate studies of potential impact of new vaccines on colonization and transmission in adolescents. METHODS: We performed plate cultures on 107 oropharyngeal swabs stored frozen in skim milk tryptone glucose glycerol (STGG) broth and previously positive for Nm. We compared quantitative polymerase chain reaction (qPCR) detection of Nm in 601 STGG-swabs with culture...
October 2016: Pediatric Infectious Disease Journal
Robert A Bednarczyk, Paulina A Rebolledo, Saad B Omer
BACKGROUND: Concerns have been raised about unauthorized immigrants importing measles to the United States (US). This potential risk has not been rigorously evaluated nor compared with the potential risk of measles importation by US residents traveling internationally or international travellers coming to the US. We compared the potential risk of measles importation from each of these populations. METHODS: Using a cross-sectional, ecological design, we compared country-level measles vaccination and incidence data, for top (i) US resident international travel destinations, (ii) US-bound international travellers' home countries and (iii) home countries of unauthorized immigrants to the US...
March 2016: Journal of Travel Medicine
Janusz Kochman, Bartosz Rymuza, Zenon Huczek, Łukasz Kołtowski, Piotr Ścisło, Radosław Wilimski, Anna Ścibisz, Paulina Stanecka, Krzysztof J Filipiak, Grzegorz Opolski
There are differences in reporting bleeding complications after transcatheter aortic valve implantation (TAVI), which is a consequence of the lack of consensus for their definition. Furthermore, the amount of data on the impact of peri-procedural bleeding on the mid-term prognosis is still limited. The aim of this study was to investigate the incidence, predictors, and impact of life-threatening and major bleedings as defined by the Valve Academic Research Consortium 2 (VARC-2) in patients after TAVI over the mid-term prognosis...
2016: International Heart Journal
Przemko Kwinta, Izabela Herman-Sucharska, Anna Leśniak, Małgorzata Klimek, Paulina Karcz, Wojciech Durlak, Magdalena Nitecka, Grażyna Dutkowska, Anna Kubatko-Zielińska, Bożena Romanowska-Dixon, Jacek Józef Pietrzyk
AIM: To assess the relationship between stereoscopic vision, visual perception, and microstructure of the corpus callosum (CC) and occipital white matter, 61 children born with a mean birth weight of 1024 g (SD 270 g) were subjected to detailed ophthalmologic evaluation, Developmental Test of Visual Perception (DTVP-3), and diffusion tensor imaging (DTI) at the age of 4. RESULTS: Abnormal stereoscopic vision was detected in 16 children. Children with abnormal stereoscopic vision had smaller CC (CC length: 53 ± 6 mm versus 61 ± 4 mm; p < 0...
2015: BioMed Research International
Joanna Majewska, Weronika Beta, Dorota Lecion, Katarzyna Hodyra-Stefaniak, Anna Kłopot, Zuzanna Kaźmierczak, Paulina Miernikiewicz, Agnieszka Piotrowicz, Jarosław Ciekot, Barbara Owczarek, Agnieszka Kopciuch, Karolina Wojtyna, Marek Harhala, Mateusz Mąkosa, Krystyna Dąbrowska
A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by 112 days without the phage, and subsequent second application of phage up to day 240. Serum and gut antibodies (IgM, IgG, secretory IgA) were analyzed in relation to microbiological status of the animals. T4 phage applied orally induced anti-phage antibodies when the exposure was long enough (IgG day 36, IgA day 79); the effect was related to high dosage...
August 2015: Viruses
Maria Kalina, Barbara Kalina-Faska, Katarzyna Gruszczyńska, Jan Baron, Anita Cisowska, Karolina Morawiec, Paulina Biel-Lewandowska, Ewa Małecka-Tendera
BACKGROUND: Structural defects of the hypothalamic-pituitary area in MRI are suggested as being a more accurate marker of growth hormone deficiency (GHD) than laboratory assays. OBJECTIVE: To compare auxological characteristics in GHD children with normal pituitary (NP) function and with ectopic posterior pituitary (EPP), prior to therapy with recombinant human growth hormone (rhGH), extending the follow-up to two years following treatment. DESIGN: Eighty-six (86) GHD patients were divided into two groups depending on the pituitary MRI: the EPP (23 children, 3...
July 2015: Hormones: International Journal of Endocrinology and Metabolism
Paulina Jóźwiak, Ewelina Wierzejska, Aleksandra Szmagaj, Maria Biskupska
Tobacco smoking has long been one of the most important risk factors contributing to the development of many health problems e.g. cardiovascular diseases respiratory diseases and cancers. Smoking is a modifiable factor, which means that every smoker who quit smoking has a great chance to lower the risk of developing these diseases. The aim of the study was to identify the factors influencing smoking among people over 45 and to estimate the extent of the phenomenon of smoking in 2 Polish provinces: Wielkopolskie and Dolnośląskie...
2014: Przegla̧d Lekarski
Elżbieta Kramarz, Ryszard Piotrowicz, Krystian Krzyzanowski, Paulina Kramarz
BACKGROUND: The reliability of noninvasive Holter method for the calculation of sinoatrial conduction time (SACT) has not been confirmed by results of invasive electrophysiological studies of sinus nodal function. The aim of this study was to compare the values of SACT obtained by Holter method with values estimated by premature atrial stimulation method. METHODS: The study population consisted of 61 patients, in whom the 24-hour ambulatory ECG monitoring had shown the occurrence of atrial premature beats...
May 2015: Annals of Noninvasive Electrocardiology
Kristen M Forney, Lauren S Polansky, Paulina A Rebolledo, Katherine Foy Huamani, Katherine E Mues, Usha Ramakrishnan, Juan S Leon
BACKGROUND: Nutrition rehabilitation centers (NRCs) have shown mixed results in reducing morbidity and mortality among undernourished children in the developing world. Follow-up on children after leaving these programs remains undocumented. OBJECTIVE: To assess the nutritional improvement of children attending the Centro de Rehabilitación Infantil Nutricional (CRIN), a residential NRC in rural Bolivia, from entrance to exit and to a household follow-up visit 1 month to 6 years later, and to identify factors associated with nutritional improvement...
June 2014: Food and Nutrition Bulletin
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