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https://www.readbyqxmd.com/read/28338054/identification-of-hexose-kinase-genes-in-kluyveromyces-marxianus-and-thermo-tolerant-one-step-producing-glucose-free-fructose-strain-construction
#1
Guorong Zhang, Min Lu, Jichao Wang, Dongmei Wang, Xiaolian Gao, Jiong Hong
In yeast, the hexose assimilation is started at hexose phosphorylation. However, in Kluyveromyces marxianus, the hexokinase (HXK) and glucokinase (GLK) genes were not identified by experiments. Meanwhile, the glucose-free fructose product requires more cost-efficient method. In this study, the KmHXK1 and KmGLK1 genes were functionally identified through gene disruption, over-expression and recombinant enzymes characterization. Both glucose and fructose assimilation ability decreased significantly in KmHXK1 disrupted strain YLM001, however, this ability was not changed obviously in KmGLK1 disrupted strain YLM002...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28323047/biochemical-and-chemical-characterization-of-cynara-cardunculus-l-extract-and-its-potential-use-as-co-adjuvant-therapy-of-chronic-myeloid-leukemia
#2
Antonio Russo, Mariarita Perri, Erika Cione, Maria Luisa Di Gioia, Monica Nardi, Maria Cristina Caroleo
ETHNOPHARMACOLOGICAL RELEVANCE: Ancient mediterranean diet was characterized by consuming the spontaneous forms of Cynara Cardunculus L. (CCL), commonly called artichoke. Cultivated and/or spontaneous forms of CC studies have demonstrated that methanol extract of CCL flower and/or cynaropicrin showed remarkable anti-proliferative activity in vitro models of leukocyte cancer cell. AIM OF THE STUDY: Chronic myeloid leukemia (CML) is associated with a reciprocal translocation of the long arms of chromosomes 9 and 22 generating the BCR/ABL fusion gene, translated in the p210(BCR/ABL) oncoprotein kinase...
March 18, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28315963/enhancement-of-2-3-butanediol-production-from-jerusalem-artichoke-tuber-extract-by-a-recombinant-bacillus-sp-strain-brc1-with-increased-inulinase-activity
#3
Jang Min Park, Baek-Rock Oh, In Yeong Kang, Sun-Yeon Heo, Jeong-Woo Seo, Seung-Moon Park, Won-Kyung Hong, Chul Ho Kim
A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp...
March 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28298683/fructan-fructan-1-fructosyltransferase-and-inulin-hydrolase-activities-relating-to-inulin-and-soluble-sugars-in-jerusalem-artichoke-helianthus-tuberosus-linn-tubers-during-storage
#4
Sukanya Maicaurkaew, Sanun Jogloy, Bruce R Hamaker, Suwayd Ningsanond
Influences of harvest time and storage conditions on activities of fructan:fructan1-fructosyltransferase (1-FFT) and inulin hydrolase (InH) in relation to inulin and soluble sugars of Jerusalem artichoke (Helianthus tuberosus L.) tubers were investigated. Maturity affected 1-FFT-activity, inulin contents, and inulin profiles of the tubers harvested between 30 and 70 days after flowering (DAF). Decreases in 1-FFT activity, high molecular weight inulin, and inulin content were observed in late-harvested tubers...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28288420/molecular-characteristics-of-water-extractable-organic-matter-from-different-composted-biomasses-and-their-effects-on-seed-germination-and-early-growth-of-maize
#5
Hiarhi Monda, Vincenza Cozzolino, Giovanni Vinci, Riccardo Spaccini, Alessandro Piccolo
Four water extractable organic matter (WEOM) were obtained from composts made out of residues of: 1. artichoke (C-CYN), 2. artichoke/fennel (C-CYNF), 3. tomato/woodchips (C-TOM), 4. Municipal solid waste (C-MSW), and their bioactivity was tested for maize seed germination and maize seedling growth. The molecular properties of both original composts and their WEOM were characterized by spectroscopic ((13)C-CPMAS- and (1)H NMR, FTIR-ATR), thermochemolysis-GC/MS, and thermal methods (TGA, DSC). While all WEOM had significant effects on plant growth, CYN-WEOM was the only material that concomitantly increased germination rate and primary and lateral root length of maize seedlings...
March 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28276907/dietary-sources-of-polyphenols-in-the-mediterranean-healthy-eating-aging-and-lifestyle-meal-study-cohort
#6
Justyna Godos, Stefano Marventano, Antonio Mistretta, Fabio Galvano, Giuseppe Grosso
The aim of this study was to estimate the dietary intake and major food sources of polyphenols in the Mediterranean healthy Eating, Aging and Lifestyles (MEAL) study cohort. A total of 1937 individuals (18 + y) of urban population of Catania, Italy, completed a validated 110-item food frequency questionnaire; Phenol-Explorer database was used to estimate polyphenol intake. Mean intake of polyphenols was 663.7 mg/d; the most abundant classes were phenolic acids (362.7 mg/d) and flavonoids (258.7 mg/d)...
February 9, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28274427/determination-of-pesticide-residues-in-globe-artichoke-leaves-and-fruits-by-gc-ms-and-lc-ms-ms-using-the-same-quechers-procedure
#7
Ignacio Machado, Natalia Gérez, Mariela Pistón, Horacio Heinzen, María Verónica Cesio
Aiming to select the most suitable sample preparation for the multiresidue analysis of pesticides in globe artichoke (Cynara cardunculus L.), a modified QuEChERS, a matrix solid phase dispersion and a dispersive ethyl acetate extraction were compared. Trueness and precision were determined at 0.2mgkg(-1) for the three methods. The modified QuEChERS showed the best performance. The scope of the method was enlarged to 35 GC and 63 LC amenable pesticides, its overall performance was evaluated and validated to artichoke leaves and fruits according to DG-SANTE Guidelines...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28261245/effect-of-spectral-quality-of-monochromatic-led-lights-on-the-growth-of-artichoke-seedlings
#8
Roel C Rabara, Glenn Behrman, Thomas Timbol, Paul J Rushton
Indoor farming is becoming a popular alternative approach in food production to meet the demand of a growing world population. Under this production system, artificial light provides the main source of illumination in sustaining plant growth and development. The use of light-emitting diodes (LEDs) is a popular source of artificial light for indoor farms due to its narrow light spectra, modular design and energy efficiency. This study purposely assessed the effect of monochromatic LED light quality on the growth of three varieties of artichoke seedlings compared to greenhouse condition...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28247096/next-generation-sequencing-and-molecular-analysis-of-artichoke-italian-latent-virus
#9
Toufic Elbeaino, Imen Belghacem, Tiziana Mascia, Donato Gallitelli, Michele Digiaro
Next-generation sequencing (NGS) allowed the assembly of the complete RNA-1 and RNA-2 sequences of a grapevine isolate of artichoke Italian latent virus (AILV). RNA-1 and RNA-2 are 7,338 and 4,630 nucleotides in length excluding the 3' terminal poly(A) tail, and encode two putative polyproteins of 255.8 kDa (p1) and 149.6 kDa (p2), respectively. All conserved motifs and predicted cleavage sites, typical for nepovirus polyproteins, were found in p1 and p2. AILV p1 and p2 share high amino acid identity with their homologues in beet ringspot virus (p1, 81% and p2, 71%), tomato black ring virus (p1, 79% and p2, 63%), grapevine Anatolian ringspot virus (p1, 65% and p2, 63%), and grapevine chrome mosaic virus (p1, 60% and p2, 54%), and to a lesser extent with other grapevine nepoviruses of subgroup A and C...
February 28, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28216099/the-draft-genome-sequence-of-the-ascomycete-fungus-penicillium-subrubescens-reveals-a-highly-enriched-content-of-plant-biomass-related-cazymes-compared-to-related-fungi
#10
Mao Peng, Adiphol Dilokpimol, Miia R Mäkelä, Kristiina Hildén, Sander Bervoets, Robert Riley, Igor V Grigoriev, Matthieu Hainaut, Bernard Henrissat, Ronald P de Vries, Zoraide Granchi
Here we report the genome sequence of the ascomycete saprobic fungus Penicillium subrubescens FBCC1632/CBS132785 isolated from a Jerusalem artichoke field in Finland. The 39.75Mb genome containing 14,188 gene models is highly similar for that reported for other Penicillium species, but contains a significantly higher number of putative carbohydrate active enzyme (CAZyme) encoding genes.
March 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28212860/comparative-structure-function-characterization-of-the-saposin-like-domains-from-potato-barley-cardoon-and-arabidopsis-aspartic-proteases
#11
Brian C Bryksa, Douglas A Grahame, Rickey Y Yada
The present study characterized the aspartic protease saposin-like domains of four plant species, Solanum tuberosum (potato), Hordeum vulgare L. (barley), Cynara cardunculus L. (cardoon; artichoke thistle) and Arabidopsis thaliana, in terms of bilayer disruption and fusion, and structure pH-dependence. Comparison of the recombinant saposin-like domains revealed that each induced leakage of bilayer vesicles composed of a simple phospholipid mixture with relative rates Arabidopsis>barley>cardoon>potato...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28190352/polyphenol-interactions-mitigate-the-immunogenicity-and-allergenicity-of-gliadins
#12
Maxime Pérot, Roberta Lupi, Sylvain Guyot, Carine Delayre-Orthez, Pascale Gadonna-Widehem, Jean-Yves Thébaudin, Marie Bodinier, Colette Larré
Wheat allergy is an IgE-mediated disorder. Polyphenols, which are known to interact with certain proteins, could be used to reduce allergic reactions. This study screened several polyphenol sources for their ability to interact with gliadins, mask epitopes, and affect basophil degranulation. Polyphenol extracts from artichoke leaves, cranberries, apples, and green tea leaves were examined. Of these extracts, the first three formed insoluble complexes with gliadins. Only the cranberry and apple extracts masked epitopes in dot blot assays using anti-gliadin IgG and IgE antibodies from patients with wheat allergies...
March 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28177802/plant-growth-promotion-properties-of-bacterial-strains-isolated-from-the-rhizosphere-of-the-jerusalem-artichoke-helianthus-tuberosus-l-adapted-to-saline-alkaline-soils-and-their-effect-on-wheat-growth
#13
Xiaolin Liu, Xiangyue Li, Yan Li, Runzhi Li, Zhihong Xie
The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta...
November 14, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28147701/genotoxic-and-chemopreventive-assessment-of-cynara-scolymus-l-aqueous-extract-in-a-human-derived-liver-cell-line
#14
Regiane Pereira da Silva, Laura Vicedo Jacociunas, Raíne Fogliati de Carli, Bianca Regina Ribas de Abreu, Mauricio Lehmann, Juliana da Silva, Alexandre de Barros Falcão Ferraz, Rafael Rodrigues Dihl
Cynara scolymus L., popularly known as artichoke, is consumed as food and used as tea infusions for pharmacological purposes to treat liver dysfunctions and other conditions. Scientific data on the safety and protective effect of artichoke in human-derived liver cells is missing. This study investigated the genotoxic and modulatory effect of a liophilized extract suspended in water of C. scolymus L. leaves. Four extract concentrations (0.62, 1.25, 2.5 and 5.0 mg/mL) were evaluated using the comet assay on human hepatocyte cultures, HepG2 cells...
February 1, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28139048/simultaneous-quantitative-determination-of-11-sesquiterpene-lactones-in-jerusalem-artichoke-helianthus-tuberosus-l-leaves-by-ultra-high-performance-liquid-chromatography-with-quadrupole-time-of-flight-mass-spectrometry
#15
Xiaoyan Yuan, Qianxu Yang
A method of ultra high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was developed for the simultaneous quantification of 11 sesquiterpene lactones in 11 Jerusalem artichoke leaf samples harvested in a number of areas at different periods. The optimal chromatographic conditions were achieved on a ZORBAX Eclipse Plus C18 column (3.0×150 mm, 1.8 microns) with linear gradient elution of methanol and water in 8 min. Quantitative analysis was carried out under selective ion monitoring mode...
January 31, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28134269/conversion-from-long-term-cultivated-wheat-field-to-jerusalem-artichoke-plantation-changed-soil-fungal-communities
#16
Xingang Zhou, Jianhui Zhang, Danmei Gao, Huan Gao, Meiyu Guo, Li Li, Mengliang Zhao, Fengzhi Wu
Understanding soil microbial communities in agroecosystems has the potential to contribute to the improvement of agricultural productivity and sustainability. Effects of conversion from long-term wheat plantation to Jerusalem artichoke (JA) plantation on soil fungal communities were determined by amplicon sequencing of total fungal ITS regions. Quantitative PCR and PCR-denaturing gradient gel electrophoresis were also used to analyze total fungal and Trichoderma spp. ITS regions and Fusarium spp. Ef1α genes...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28127803/artichoke-leaf-extract-inhibits-akr1b1-and-reduces-nf-%C3%AE%C2%BAb-activity-in-human-leukemic-cells
#17
Ivana Miláčková, Kristína Kapustová, Pavel Mučaji, Jan Hošek
The human intracellular enzyme AKR1B1 belongs to the aldo-keto reductase superfamily. The AKR1B1-catalyzed reduction of aldehydes is part of the intracellular inflammatory pathway leading to the activation of NF-κB and the expression of pro-inflammatory genes. The present study is aimed at determining the inhibition of AKR1B1 brought about by an extract of artichoke leaves (bracts), and the effects of this extract and three participating compounds on the expression of AKR1B1, COX-2, and MMP-2 proteins in THP-1 cells...
January 26, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28032139/format-change-and-semantic-relatedness-effects-on-the-erp-correlates-of-recognition-old-pairs-new-pairs-different-stories
#18
Fabrice Guillaume, Sophia Baier, Mélanie Bourgeois, Sophie Tinard
In this event-related potential (ERP) study, we investigated the effects of format change and semantic relatedness in a recognition task using pairs composed of a word and a line drawing. The semantic relatedness of the pairs (related: rabbit-carrot; unrelated: duck-artichoke) influenced their associative properties and corresponding distinctiveness, while format change refers to the switching of an item from the verbal form to the line drawing form between study and recognition (e.g., the word "egg" is associated with a drawing of a hen at study, and a line drawing of an egg is associated with the word "hen" at test)...
April 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/28017989/antioxidant-effects-of-supercritical-fluid-garlic-extracts-in-canned-artichokes
#19
E Bravi, O Marconi, V Sileoni, M R Rollo, G Perretti
The effects of adding supercritical carbon dioxide extracts of garlic (at two different concentrations of allicin) on select chemical indices in extra-virgin olive oil used to canned artichokes were studied. Tests were performed after processing and over a storage period of 1 year. A sensorial test was also conducted on the canned artichokes to establish the impact on flavor (in particular perceptions of rancidity and garlic flavor). Acidity, peroxide levels and p-anisidine values were measured as quality analytical parameters...
October 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28008316/cynaropicrin-a-comprehensive-research-review-and-therapeutic-potential-as-an-anti-hepatitis-c-virus-agent
#20
REVIEW
Mahmoud F Elsebai, Andrei Mocan, Atanas G Atanasov
The different pharmacologic properties of plants-containing cynaropicrin, especially artichokes, have been known for many centuries. More recently, cynaropicrin exhibited a potential activity against all genotypes of hepatitis C virus (HCV). Cynaropicrin has also shown a wide range of other pharmacologic properties such as anti-hyperlipidemic, anti-trypanosomal, anti-malarial, antifeedant, antispasmodic, anti-photoaging, and anti-tumor action, as well as activation of bitter sensory receptors, and anti-inflammatory properties (e...
2016: Frontiers in Pharmacology
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