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https://www.readbyqxmd.com/read/29149771/hoxc13-and-hsp27-expression-in-skin-and-the-periodic-growth-of-secondary-fiber-follicles-from-longdong-cashmere-goats-raised-in-different-production-systems
#1
Jiqing Wang, Ping Yu, Haifang Wang, Yanyu He
This experiment was conducted to identify periodic changes in secondary follicles (SFs) over one year of extensive (grazed) and intensive (housed and fed supplement) farming of Longdong cashmere goats. One-year old goats (n = 24, 12 extensively fed and 12 intensively fed) were studied. The diameter, depth, density, activity, and ultra-structural features of SFs were assessed using light microscopy and transmission electron microscopy (TEM). HOXC13 and HSP27 expression were studied using immunohistochemistry and immunofluorescence method...
November 17, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29145799/gangrenous-dermatitis-in-chickens-and-turkeys
#2
Carlos D Gornatti-Churria, Manuela Crispo, H L Shivaprasad, Francisco A Uzal
Gangrenous dermatitis (GD) is a disease of chickens and turkeys that causes severe economic losses in the poultry industry worldwide. Clostridium septicum, Clostridium perfringens type A, and occasionally Clostridium sordellii are considered the main causes of GD, although Staphylococcus aureus and other aerobic bacteria may also be involved in some cases of the disease. GD has become one of the most significant diseases of commercial turkeys in the United States. Several infectious and/or environmental immunosuppressive factors can predispose to GD...
November 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29145798/experimental-transmission-of-bovine-digital-dermatitis-to-sheep-development-of-an-infection-model
#3
Jennifer H Wilson-Welder, Jarlath E Nally, David P Alt, Mitchell V Palmer, John Coatney, Paul Plummer
Digital dermatitis is an infectious cause of lameness primarily affecting cattle but also described in sheep, goats, and wild elk. Digital dermatitis is a polymicrobial infection, involving several Treponema species and other anaerobic bacteria. Although the exact etiology has not been demonstrated, a number of bacterial, host, and environmental factors are thought to contribute to disease development. To study host-bacterial interactions, a reproducible laboratory model of infection is required. The objective of this study was to demonstrate key aspects of bovine digital dermatitis lesions in an easy-to-handle sheep model...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/29145794/analysis-of-bacterial-and-fungal-nucleic-acid-in-canine-sterile-granulomatous-and-pyogranulomatous-dermatitis-and-panniculitis
#4
Fabio B Rosa, Caitlin E Older, Courtney Meason-Smith, Jan S Suchodolski, Sonia Lingsweiler, Joanne E Mansell, Aline Rodrigues Hoffmann
Next generation sequencing (NGS) studies are revealing a diverse microbiota on the skin of dogs. The skin microbiota of canine sterile granulomatous and pyogranulomatous dermatitis (SGPD) has yet to be investigated using NGS techniques. NGS targeting the 16S rRNA and ITS-1 region of bacterial and fungal DNA, respectively, were used to investigate if bacterial and fungal DNA were associated with skin lesions in cases of canine SGPD. The study included 20 formalin-fixed paraffin-embedded (FFPE) skin samples and 12 fresh samples from SGPD-affected dogs, and 10 FFPE and 10 fresh samples from healthy dogs...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/29144832/postoperative-complications-of-dermis-fat-autografts-in-the-anophthalmic-socket
#5
Victoria Starks, Suzanne K Freitag
Reconstruction of the anophthalmic socket allows the use of an ocular prosthesis and rehabilitation of facial appearance. Dermis-fat grafting is one option in volume augmentation of the anophthalmic socket and presents unique benefits, including increased surface area within the socket and the ability to grow with pediatric patients. Postoperative complications of this procedure are relatively common. Minor complications, such as graft hirsutism, keratinization, and conjunctival cysts or granulomas, are managed easily by observation or simple intervention...
November 16, 2017: Seminars in Ophthalmology
https://www.readbyqxmd.com/read/29143381/comparing-dermoscopy-and-histological-examination-of-normal-equine-skin
#6
Lara M Tomich, Jason B Pieper, Adam W Stern
BACKGROUND: Dermoscopy is a noninvasive diagnostic technique that allows visualization of structures of the superficial dermis not visible with the naked eye. HYPOTHESIS/OBJECTIVES: To assess the usefulness and applicability of dermoscopy for evaluation of healthy equine skin. ANIMALS: Twelve healthy horses from a research herd. METHODS: Five regions (cheek, lateral neck, dorsum, flank and abdomen) were examined with contact dermoscopy using both nonpolarized and polarized light at both 17-fold and 24-fold magnification...
November 15, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/29142430/topical-delivery-of-withania-somnifera-crude-extracts-in-niosomes-and-solid-lipid-nanoparticles
#7
Tawona N Chinembiri, Minja Gerber, Lissinda H du Plessis, Jan L du Preez, Josias H Hamman, Jeanetta du Plessis
Background: Withania somnifera is a medicinal plant native to India and is known to have anticancer properties. It has been investigated for its anti-melanoma properties, and since melanoma presents on the skin, it is prudent to probe the use of W. somnifera in topical formulations. To enhance topical drug delivery and to allow for controlled release, the use of niosomes and solid lipid nanoparticles (SLNs) as delivery vesicles were explored. Objective: The objective of this study is to determine the stability and topical delivery of W...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29141956/rapid-isolation-and-expansion-of-skin-derived-precursor-cells-from-human-primary-fibroblast-cultures
#8
Leithe Budel, Karima Djabali
Skin-derived precursor (SKP) cells have self-renewal and multipotent abilities and are found in the dermis. SKP cells have been isolated previously from pre-established dermal fibroblast cultures. In these procedures, long-term culture and low yield remain the crucial aspects requiring improvement. In this study, we exposed pre-established dermal fibroblasts to 30-min acid stress prior to isolating SKP cells (termed pH-SKP) and compared the yield to the previously published trypsin- and no-stress methods. Spheroid formation was confirmed and analyzed at days 3, 5 and 7...
November 15, 2017: Biology Open
https://www.readbyqxmd.com/read/29141064/port-wine-stains-a-focused-review-on-their-management
#9
Katelyn Mariko Updyke, Amor Khachemoune
<p>Port-wine stain (PWS) is the second most common congenital vascular malformation characterized as ectatic capillaries and venules in the dermis that clinically appears as a deep red to purple patch on the skin. Typically, PWS progressively darken and may become hypertrophic or nodular without treatment. There are several treatment options available for PWS from topical antiangiogenic agents to laser therapies. Vascular-specific lasers are the gold standard in treating PWS and classically pulsed dye lasers are usually the treatment of choice...
November 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29140982/development-standardization-and-testing-of-a-bacterial-wound-infection-model-based-on-ex-vivo-human-skin
#10
Christoph Schaudinn, Christin Dittmann, Jana Jurisch, Michael Laue, Nazende Günday-Türeli, Ulrike Blume-Peytavi, Annika Vogt, Fiorenza Rancan
Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using-otherwise discarded-skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis...
2017: PloS One
https://www.readbyqxmd.com/read/29140869/rhinophyma-a-treatment-review
#11
Caitlin Fink, Jeffrey Lackey, Donald J Grande
BACKGROUND: Rhinophyma is the overgrowth of sebaceous glands in nasal tissue and its etiology unclear. Without treatment, rhinophyma can be progressive and cause concern both with respect to function and cosmesis. OBJECTIVE: The objective of this work is to describe treatment options for rhinophyma and their respective risks and benefits. MATERIALS AND METHODS: A PubMed search was performed to include the terms "rhinophyma" and "treatment...
November 14, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29139361/angiokeratoma-of-the-glans-penis
#12
Bryce D Beutler, Philip R Cohen
Angiokeratomas are benign vascular lesions characterized by ectatic blood vessels in the papillary dermis with overlying acanthosis and hyperkeratosis, often occurring as idiopathic scrotal tumor and rarely as penile lesions. Twelve men, including our case of a 58-year-old white man, have been reported with angiokeratomas of the glans penis. The lesions typically present as asymptomatic dome-shaped papules ranging in size from 0.5 to 5 mm and present as red, purple, blue, or black lesions along the penile coronal rim...
2017: Skinmed
https://www.readbyqxmd.com/read/29139245/evaluation-of-a-pointwise-microcirculation-assessment-method-using-liquid-and-multilayered-tissue-simulating-phantoms
#13
Ingemar Fredriksson, Rolf B Saager, Anthony J Durkin, Tomas Strömberg
A fiber-optic probe-based instrument, designed for assessment of parameters related to microcirculation, red blood cell tissue fraction (fRBC), oxygen saturation (SO2), and speed resolved perfusion, has been evaluated using state-of-the-art tissue phantoms. The probe integrates diffuse reflectance spectroscopy (DRS) at two source-detector separations and laser Doppler flowmetry, using an inverse Monte Carlo method for identifying the parameters of a multilayered tissue model. Here, we characterize the accuracy of the DRS aspect of the instrument using (1) liquid blood phantoms containing yeast and (2) epidermis-dermis mimicking solid-layered phantoms fabricated from polydimethylsiloxane, titanium oxide, hemoglobin, and coffee...
November 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29135510/mycosis-fungoides-manifesting-as-giant-cell-lichenoid-dermatitis
#14
Irena E Belousova, Vladislav R Khairutdinov, Anna Bessalova, Dmitry V Kazakov
Mycosis fungoides (MF) is the most common form of primary cutaneous lymphoma with a broad clinicopathological spectrum. Unusual histopathologic patterns of MF include lichenoid, interstitial, folliculotropic, spongiotic, granulomatous, and many others. Several cases of unusual lichenoid reaction characterized by a mixed lichenoid inflammatory infiltrate with prominent infiltration of the papillary dermis and epidermis by multinucleated giant cells were described under the name of "giant cell lichenoid dermatitis," most of them were considered to represent a drug eruption...
November 10, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29135509/unilesional-follicular-mycosis-fungoides-report-of-6-cases-and-review-of-the-literature
#15
Cynthia M Magro, Gladys H Telang, Shabnam Momtahen
Unilesional follicular mycosis fungoides (MF) is a rare variant of cutaneous T-cell lymphoma characterized by selective involvement of the hair follicles by neoplastic T lymphocytes presenting initially as a solitary lesion occupying less than 5% of the body surface; there are 22 previous reported cases. We describe 6 patients with this rare variant of MF, all males (age range 6-64 years; mean age: 28 years) presenting with a solitary lesion primarily on the face and scalp except 1 patient who presented with a truncal lesion...
October 24, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29134755/analysis-of-the-influence-of-collagen-fibres-in-the-dermis-on-skin-optical-reflectance-by-monte-carlo-simulation-in-a-nine-layered-skin-model
#16
Y Masuda, Y Ogura, Y Inagaki, T Yasui, Y Aizu
BACKGROUND: Collagen fibres in the dermis play an important structural role in the skin. Age-related changes to these fibres cause wrinkles and slackness of facial skin. However, it is not clear how dermal collagen fibres affect skin colour. The purpose of this study was to clarify the influence of altered collagen fibres on skin colour, using both experimental measurement of fibre density and Monte Carlo simulations in an optical model of skin. METHODS: Reflection spectra were measured from the cheeks of 12 Japanese women (22-65 years old) by spectral colorimeter...
November 13, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/29132097/the-development-of-a-new-artificial-model-of-a-finger-for-assessing-transmitted-vibrations
#17
Almaky Almagirby, Jem A Rongong, Matt J Carré
Prolonged exposure of the hand to tool-induced vibrations is associated with the occurrence of conditions such as vibration white finger. This study involves the development of a new artificial model that approximates both loading and vibration behaviour of the human finger. The layered system uses polypropylene "bones", encased in a cylinder of low modulus, room-temperature curing silicone gel (to replicate subcutaneous tissues), with an outer layer of latex (to replicate the dermis and epidermis). A protocol for manufacture was developed and dynamic mechanical analysis was carried out on a range of gels in order to choose a range close to the mechanical properties of the human finger...
November 4, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29131889/poisson-contraction-and-fiber-kinematics-in-tissue-insight-from-collagen-network-simulations
#18
Catalin Picu, Sai Deogekar, Mohammad Islam
Connective tissue mechanics is highly non-linear, exhibits a strong Poisson effect and is associated with significant collagen fiber re-arrangement. Although the general features of the stress-strain behavior in tension and compression and under uniaxial, biaxial and shear loading have been discussed extensively, especially from the macroscopic perspective, the Poisson effect and the kinematics of filaments have received less attention. In general, the relationship between the microscopic fiber network mechanics and the macroscopic experimental observations remains poorly defined...
November 13, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/29131564/light-emitting-photon-upconversion-nanoparticles-in-the-generation-of-transdermal-reactive-oxygen-species
#19
Martin Prieto, Alina Rwei, Teresa Alejo, Wei Tuo, Maria Teresa Lopez-Franco, Gracia Mendoza, Victor Sebastian, Daniel S Kohane, Manuel Arruebo
Common photosensitizers used in photodynamic therapy do not penetrate the skin effectively. In addition, the visible blue and red lights used to excite such photosensitizers have shallow penetration depths through tissue. To overcome these limitations, we have synthesized ultraviolet and visible light emitting, energy-transfer based upconversion nanoparticles, and co-encapsulated them inside PLGA-PEG nanoparticles with photosensitizer protoporphyrin IX. Nd3+ has been introduced as sensitizer in the upconversion nanostructures to allow their excitation at 808 nm...
November 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29130677/-dermoscopy-study-on-the-effects-of-gold-microacupuncture-hyaluronic-acid-injection-and-rb-bfgf-to-treat-facial-rejuvenation
#20
Ge Su, Xun Zhou
OBJECTIVE: To explore the dermoscopic changes facial rejuvenation after the combination therapy of gold microacupuncture,Hyaluronic acid (HA) injection,and recombinant bovine alkaline fibroblast growth factor (rbbFGF). METHODS: Twenty cases of patients (age from 30 to 55 yearold) with facial skin naturally aging were included. The patients received multisource radio frequency therapeutic apparatus for injection at the subbermaldermal levels from 1316 W. Noncrosslinking HA of 2 mL was injected in the upper dermis...
September 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
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