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Robert Nawrot, Jakub Barylski, Rico Lippmann, Lothar Altschmied, Hans-Peter Mock
A novel annotated Chelidonium majus L. transcriptome database composed of 23,004 unique coding sequences allowed to significantly improve the sensitivity of proteomic C. majus assessments, which showed novel defense-related proteins characteristic to its latex. To date, the composition of Chelidonium majus L. milky sap and biosynthesis of its components are poorly characterized. We, therefore, performed de novo sequencing and assembly of C. majus transcriptome using Illumina technology. Approximately, 119 Mb of raw sequence data was obtained...
July 11, 2016: Planta
Milena Deljanin, Mladen Nikolic, Dejan Baskic, Danijela Todorovic, Predrag Djurdjevic, Milan Zaric, Milan Stankovic, Milos Todorovic, Dusko Avramovic, Suzana Popovic
ETHNOPHARMACOLOGICAL RELEVANCE: Chelidonium majus L (Papaveraceae) is widely used in alternative medicine for treatment of various disorders. Antitumor activities of alkaloids isolated from this plant have been reviewed, while there are only a few studies that examine properties of the whole extract. AIM OF THE STUDY: The aim of the present study was to investigate direct cytotoxic effects, as well as indirect antitumor effects of Chelidonium majus ethanolic extract against different tumor cell lines,...
August 22, 2016: Journal of Ethnopharmacology
Radim Havelek, Martina Seifrtova, Karel Kralovec, Eliska Krocova, Veronika Tejkalova, Ivan Novotny, Lucie Cahlikova, Marcela Safratova, Lubomir Opletal, Zuzana Bilkova, Jirina Vavrova, Martina Rezacova
BACKGROUND: The search for new anticancer compounds is a crucial element of natural products research. PURPOSE: In this study the effects of naturally occurring homochelidonine in comparison to chelidonine on cell cycle progression and cell death in leukemic T-cells with different p53 status are described. METHODS: The mechanism of cytotoxic, antiproliferative, apoptosis-inducing effects and the effect on expressions of cell cycle regulatory proteins was investigated using XTT assay, Trypan blue exclusion assay, flow cytometry, Western blot analysis, xCELLigence, epi-fluorescence and 3D super resolution microscopy...
March 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Przemysław Łukasz Mikołajczak, Bogdan Kędzia, Marcin Ożarowski, Radosław Kujawski, Anna Bogacz, Joanna Bartkowiak-Wieczorek, Wojciech Białas, Agnieszka Gryszczyńska, Waldemar Buchwald, Michał Szulc, Natalia Wasiak, Małgorzata Górska-Paukszta, Justyna Baraniak, Bogusław Czerny, Agnieszka Seremak-Mrozikiewicz
The aim of the study was to evaluate analgesic activity ("hot plate" test), anti-inflammatory activity (carrageenan-induced paw edema) and locomotor activity in rats under the influence of three fractions of Chelidonium majus herb extract: full water extract (FWE), protein enriched fraction (PEF), and non-protein fraction (NPF). Effects of the fractions on the level of chosen cytokines and their mRNA levels were also assessed using lipopolysaccharide (LPS) administration as a proinflammatory cue. All fractions and diclofenac did not affect the locomotor activity of rats in comparison with the control group...
2015: Central-European Journal of Immunology
Cengiz Kocak, Fatma Emel Kocak, Raziye Akcilar, Aydin Akcilar, Bircan Savran, Sezgin Zeren, Zulfu Bayhan, Zeynep Bayat
Intestinal ischemia-reperfusion (I/R) causes severe destruction in remote organs. Lung damage is a frequently seen complication after intestinal I/R. Ukrain (NSC 631570) is a synthetic thiophosphate derivative of alkaloids from the extract of the celandine (Chelidonium majus L.) plant. We investigated the effect of Ukrain in animals with lung injury induced by intestinal I/R. Adult male Spraque-Dawley rats were randomly divided into four groups: control, Ukrain, I/R, I/R with Ukrain. Before intestinal I/R was induced, Ukrain was administered intraperitoneally at a dose of 7...
2016: Bosnian Journal of Basic Medical Sciences
Wioleta Jesionek, Emilia Fornal, Barbara Majer-Dziedzic, Ágnes M Móricz, Wassil Nowicky, Irena M Choma
The greater celandine (Chelidonium majus L.) has been known for the centuries as a medicinal plant. One of the therapeutic agents based on C. majus is anticancer drug Ukrain™ known as a semi-synthetic C. majus alkaloid derivative. Although there are no doubts about antitumor properties of the drug, there is still controversy about its composition. In this study, Ukrain™ was subjected to TLC and LC-MS/MS analyses to compare it with C. majus alkaloid root extract and to determine its composition. Moreover, microbiological activity of both Ukrain™ and the alkaloid extract were tested against Bacillus subtilis strains using TLC-direct bioautography...
January 15, 2016: Journal of Chromatography. A
Rica Capistrano I, An Wouters, Filip Lardon, Claudia Gravekamp, Sandra Apers, Luc Pieters
BACKGROUND: Chelidonium majus L. (Papaveraceae) (greater celandine) is a medicinal herb that is widely spread in Europe. Antitumoural activity has been reported for C. majus extracts. HYPOTHESIS/PURPOSE: To investigate the antitumour activity of a C. majus extract in vitro and in vivo. STUDY DESIGN: Cytotoxic effects of C. majus extracts were evaluated on human cancer cell lines, i.e. PANC-1 (pancreas cancer), HT-29 (colon cancer), MDA-MB-231 (breast cancer), PC-EM005 and PC-EM002 (primary endometrium cancer cells), and PANC02 (murine pancreatic adenocarcinoma cells)...
December 15, 2015: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Sergey O Tcherniuk, Olga Chesnokova, Irina V Oleinikov, Anatoly I Potopalsky, Andrew V Oleinikov
BACKGROUND: Malaria caused by Plasmodium falciparum is the most virulent form of malaria, leading to approximately a half million deaths per year. Chemotherapy continues to be a key approach in malaria prevention and treatment. Due to widespread parasite drug resistance, identification and development of new anti-malarial compounds remains an important task of malarial parasitology. The semi-synthetic drug amitozyn, obtained through alkylation of major celandine (Chelidonium majus) alkaloids with N,N'N'-triethylenethiophosphoramide (ThioTEPA), is a widely used Eastern European folk medicine for the treatment of various tumours...
October 29, 2015: Malaria Journal
Seung-Hyung Kim, Jung-Hee Hong, Young-Cheol Lee
BACKGROUND: Chelidonine, a major bioactive, isoquinoline alkaloid ingredient in Chelidonium majus, exhibits anti-inflammatory and other pharmacological properties. However, its molecular mechanisms in asthma remain unclear. In this work we investigated chelidonine's effect and mechanism in airway inflammation in a mouse model of allergic asthma. METHODS: The mice were sensitized to ovalbumin followed by aerosol allergen challenges and determination of chelidonine's effect on enhanced pause (Penh), pulmonary eosinophilic infiltration, eotaxin-2, interleukin-4 (IL-4), IL-13, OVA-specific IgE production, and several transcription factors...
December 2015: Pharmacological Reports: PR
Yue Sun, Yuanyuan Li, Jiajian Zeng, Qixian Lu, Paul C H Li
A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures...
September 1, 2015: Talanta
Okhwa Kim, Cheol Hwangbo, Junhyeong Kim, Dong-Hao Li, Byung-Sun Min, Jeong-Hyung Lee
Metastasis is the primary cause of cancer-associated mortality. The ternary IPP complex of integrin-linked kinase, PINCH and parvin functions as a signaling platform for integrins, which modulate numerous cellular processes including cell migration and invasion. Chelidonine, isolated from Chelidonium majus, is a benzophenanthridine alkaloid that exhibits anticancer properties; however, the anti-migratory and anti-invasive effects of chelidonine remain unknown. The aim of the present study was to investigate the inhibitory effects of chelidonine on migration and invasion of MDA-MB-231 human breast cancer cells, and to determine the underlying mechanisms...
August 2015: Molecular Medicine Reports
Miroslav Mišík, Michael Kundi, Clemens Pichler, Metka Filipic, Bernhard Rainer, Katarina Mišíková, Armen Nersesyan, Siegfried Knasmueller
Cytostatic drugs are among the most toxic chemicals which are produced. Many of them cause damage of the genetic material which may affect the fertility of higher organisms. To study the impact of the widely used anticancer drugs [cisplatin (CisPt), etoposide (Et), and 5-fluorouracil (5-FU)] on the reproduction of higher plants, pollen abortion experiments were conducted with species which belong to major plant families, namely with Tradescantia paludosa (Commelinaceae), Arabidopsis thaliana (Brassicaceae), Chelidonium majus (Papaveraceae), and Alisma plantago-aquatica (Alismataceae)...
August 2016: Environmental Science and Pollution Research International
Jana Paulsen, Mahdi Yahyazadeh, Sophie Hänsel, Maik Kleinwächter, Kerstin Ibrom, Dirk Selmar
The genuine major benzylisoquinoline alkaloid occurring in the traditional medicinal plant greater celandine (Chelidonium majus L.) is 13,14-dihydrocoptisine and not - as described previously - coptisine. Structure of 13,14-dihydrocoptisine was elucidated. The discrepancy between the alkaloid pattern of the living plants and that of detached and dried leaves is due to the rapid and prompt conversion of 13,14-dihydrocoptisine to coptisine in the course of tissue injuries. Indeed, apart from the major alkaloid, some minor alkaloids might also be converted; this however is not in the centre of focus of this paper...
March 2015: Phytochemistry
Péter Orvos, László Virág, László Tálosi, Zsuzsanna Hajdú, Dezső Csupor, Nikoletta Jedlinszki, Tamás Szél, András Varró, Judit Hohmann
Chelidonium majus or greater celandine is spread throughout the world, and it is a very common and frequent component of modern phytotherapy. Although C. majus contains alkaloids with remarkable physiological effect, moreover, safety pharmacology properties of this plant are not widely clarified, medications prepared from this plant are often used internally. In our study the inhibitory effects of C. majus herb extracts and alkaloids on hERG potassium current as well as on cardiac action potential were investigated...
January 2015: Fitoterapia
A Orland, K Knapp, G M König, G Ulrich-Merzenich, W Knöß
BACKGROUND AND OBJECTIVE: Even though herbal medicines have played an important role in disease management and health for many centuries, their present frequent use is challenged by the necessity to determine their complex composition and their multitarget mode of action. In the present study, modern methods were investigated towards their potential in the characterization of herbal substances. As a model the herbal substance Chelidonii herba was used, for which several reports on liver toxicities exist...
October 15, 2014: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Seung-Won Park, Seong Ryul Kim, Youngchul Kim, Jang-Hoon Lee, Hong-Jung Woo, Yeo-Kwang Yoon, Young Il Kim
Chelidonium majus L. (C. majus L.) is known to possess certain biological properties such as anti-inflammatory, antimicrobial, antiviral and antitumor activities. We investigated the effects of C. majus L. extract on human epidermoid carcinoma A431 cells through multiple mechanisms, including induction of cell cycle arrest, activation of the caspase-dependent pathway, blocking of nuclear factor-κB (NF-κB) activation and involvement in the mitogen-activated protein kinase (MAPK) pathway. C. majus L. inhibited the proliferation of A431 cells in a dose- and time-dependent manner, increased the percentage of apoptotic cells, significantly decreased the mRNA levels of cyclin D1, Bcl-2, Mcl-1 and survivin and increased p21 and Bax expression...
January 2015: Oncology Reports
Wen-Juan Zhang, Chun-Xue You, Cheng-Fang Wang, Li Fan, Ying Wang, Yang Su, Zhi-Wei Deng, Shu-Shan Du
One new alkaloid, together with 10 known compounds were isolated from the aerial parts of Chelidonium majus L. by repeated silica gel column chromatography. Their chemical structures were elucidated on the basis of physicochemical and spectroscopic data. Among them, 6-acetonyldihydrochelerythrine (4), 6-acetonyldihydrosanguinarine (5), 6-ketenesanguinarine (6), demethylchelerythrine (7) and demethylsanguinarine (11) were isolated for the first time from this plant. Compound 6 was identified as a new compound...
2014: Natural Product Research
Clara Grosso, Federico Ferreres, Angel Gil-Izquierdo, Patrícia Valentão, Maria Sampaio, Júlio Lima, Paula B Andrade
A Box-Behnken design (BBD) was developed to study the influence of four parameters (X1: % methanol; X2: extraction time; X3: extraction temperature; X4: solid/solvent ratio) on two responses, namely extraction yield and phenolics content of the aerial parts of Chelidonium majus L. The model presented a good fit to the experimental results for the extraction yield, being significantly influenced by X1 and X4. On the other hand a parameter reduction was necessary to run the model for phenolics content, showing that only X1 and X2 had great influence on the response...
December 2014: Talanta
Upma Bagai, Neha S Walter
BACKGROUND: Malaria remains a major global health concern in developing regions of the world. Homeopathy, a holistic system of medicine, has a lot to offer in protecting against malaria. METHODS: In the present study, antimalarial efficacy of combination of two homeopathic drugs Chelidonium 30 and nosode 30 has been evaluated in vivo against Plasmodium berghei (P. berghei) infection using Peters 4-day test. Biochemical assays have been performed to assess the levels of hepatic and renal function biomarkers upon drug treatment using diagnostic kits...
September 2014: Journal of Complementary & Integrative Medicine
Weifeng Li, Huimin Huang, Xiaofeng Niu, Ting Fan, Hua Hu, Yongmei Li, Huan Yao, Huani Li, Qingli Mu
Recent studies show that nuclear factor-kappa B (NF-κB) signaling pathway plays a key role in contributing to the development of lipopolysaccharide (LPS)-induced acute lung injury (ALI). Tetrahydrocoptisine is one of the main active components of Chelidonium majus L. and has been described to be effective in suppressing inflammation. The aim of the present study is to evaluate the protective effect of tetrahydrocoptisine on LPS-induced ALI in rats and clarify its underlying mechanisms of action. We found that in vivo pretreatment with tetrahydrocoptisine to rats 30 min before inducing ALI by LPS markedly decreased the mortality rate, lung wet weight to dry weight ratio, and ameliorated lung pathological changes...
December 2014: Inflammation
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