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https://www.readbyqxmd.com/read/28437777/testing-decontaminated-sediments-as-a-substrate-for-ornamentals-in-field-nursery-plantations
#1
Francesca Ugolini, Costanza Calzolari, Giuseppe Mario Lanini, Luciano Massetti, Stefania Pollaki, Antonio Raschi, Francesco Sabatini, Giacomo Tagliaferri, Fabrizio Ungaro, Daniele Massa, Maurizio Antonetti, Carlos Garcia Izquierdo, Cristina Macci, Grazia Masciandaro
When canals and harbours are dredged, huge amount of polluted sediments has to be stocked and transported to the landfill with incredibly high costs of management. Among the remediation techniques for the reclamation of polluted sediments and soils, phytoremediation represents a sustainable and effective technique though still not fully promoted or commercialized. In this study we have tested the suitability for plant nursing of a substrate resulting from sediments dredged from a canal and treated with phytoremediation...
April 21, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28428485/pathological-lesions-in-the-central-nervous-system-and-peripheral-tissues-of-ddy-mice-with-street-rabies-virus-1088-strain
#2
Kazunori Kimitsuki, Kentaro Yamada, Nozomi Shiwa, Satoshi Inoue, Akira Nishizono, Chun-Ho Park
Most studies on rabies virus pathogenesis in animal models have employed fixed rabies viruses, and the results of those employing street rabies viruses have been inconsistent. Therefore, to clarify the pathogenesis of street rabies virus (1088 strain) in mice, 10(6) focus forming units were inoculated into the right hindlimb of ddY mice (6 weeks, female). At 3 days postinoculation (DPI), mild inflammation was observed in the hindlimb muscle. At 5 DPI, ganglion cells in the right lumbosacral spinal dorsal root ganglia showed chromatolysis...
April 20, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28427342/intermittent-parathyroid-hormone-pth-promotes-cementogenesis-and-alleviates-the-catabolic-effects-of-mechanical-strain-in-cementoblasts
#3
Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang, Shujuan Zou
BACKGROUND: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment...
April 20, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28424663/antimicrobial-photodynamic-therapy-against-endodontic-enterococcus-faecalis-and-candida-albicans-mono-and-mixed-biofilms-in-the-presence-of-photosensitizers-a-comparative-study-with-classical-endodontic-irrigants
#4
Patrícia Diogo, Chantal Fernandes, Francisco Caramelo, Marta Mota, Isabel M Miranda, M A F Faustino, M G P M S Neves, Marciana P Uliana, Kleber T de Oliveira, João M Santos, Teresa Gonçalves
Endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. In this study, it was assessed the efficacy of antimicrobial Photodynamic Therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) activated by red light against monospecies and mixed biofilms of Enterococcus faecalis and Candida albicans. The results were compared with the ones obtained with Rose Bengal (RB), Toluidine Blue-O (TBO), the synthetic tetracationic porphyrin (TMPyP) as well as classical endodontic irrigants (3% NaOCl, 17% EDTA and 2% CHX)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28418107/a-new-path-in-defining-light-parameters-for-hair-growth-discovery-and-modulation-of-photoreceptors-in-human-hair-follicle
#5
Serena Buscone, Andrei N Mardaryev, Bianca Raafs, Jan W Bikker, Carsten Sticht, Norbert Gretz, Nilofer Farjo, Natallia E Uzunbajakava, Natalia V Botchkareva
BACKGROUND AND OBJECTIVE: Though devices for hair growth based on low levels of light have shown encouraging results, further improvements of their efficacy is impeded by a lack of knowledge on the exact molecular targets that mediate physiological response in skin and hair follicle. The aim of this study was to investigate the expression of selected light-sensitive receptors in the human hair follicle and to study the impact of UV-free blue light on hair growth ex vivo. MATERIAL AND METHODS: The expression of Opsin receptors in human skin and hair follicles has been characterized using RT-qPCR and immunofluorescence approaches...
April 18, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28416302/effect-of-bacterial-biofilm-on-the-osteogenic-differentiation-of-stem-cells-of-apical-papilla
#6
Lakshmi Vishwanat, Rose Duong, Koyo Takimoto, Linda Phillips, Claudia O Espitia, Anibal Diogenes, Shivani B Ruparel, David Kolodrubetz, Nikita B Ruparel
INTRODUCTION: Although clinical success in regenerative endodontics is substantially high, histological success is limited to finding bone/cementum-like tissue instead of dentin within the canal space. The aims of this study were to investigate (1) the effect of bacterial biofilm on osteogenic gene expression in stem cells of the apical papilla (SCAP) and (2) the effect of bacterial antigens on the functional differentiation of SCAP into a mineralizing phenotype. METHODS: Using an ex vivo organotypic root canal model and an American Association of Endontists-recommended regenerative endodontic procedures, we evaluated SCAP differentiation in the presence and absence of an Enterococcus faecalis biofilm...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28413317/korean-red-ginseng-improves-atopic-dermatitis-like-skin-lesions-by-suppressing-expression-of-proinflammatory-cytokines-and-chemokines-in%C3%A2-vivo-and-in%C3%A2-vitro
#7
Ji-Ye Kee, Yong-Deok Jeon, Dae-Seung Kim, Yo-Han Han, Jinbong Park, Dong-Hyun Youn, Su-Jin Kim, Kwang Seok Ahn, Jae-Young Um, Seung-Heon Hong
BACKGROUND: The prevalence of allergic inflammatory diseases such as atopic dermatitis (AD), asthma, and allergic rhinitis worldwide has increased and complete recovery is difficult. Korean Red Ginseng, which is the heat-processed root of Panax ginseng Meyer, is widely and frequently used as a traditional medicine in East Asia. In this study, we investigated whether Korean Red Ginseng water extract (RGE) regulates the expression of proinflammatory cytokines and chemokines via the mitogen-activated protein kinases (MAPKs)/nuclear factor kappa B (NF-κB) pathway in allergic inflammation...
April 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28411516/effects-of-foliar-dressing-of-selenite-and-silicate-alone-or-combined-with-different-soil-ameliorants-on-the-accumulation-of-as-and-cd-and-antioxidant-system-in-brassica-campestris
#8
Yongzhen Ding, Yongjiu Wang, Xiangqun Zheng, Weimin Cheng, Rongguang Shi, Renwei Feng
This study was conducted to investigate the possibility of using a combined technology to synchronously reduce As and Cd accumulation in the edible parts of Brassica campestris. The results showed that a foliar application of selenite (Se) and silicon (Si) combined with soil ameliorants (including Ca-Mg-P fertilizer, sodium silicate and red mud) showed limited effects on the growth of B. campestris. The As concentration in the leaves of B. campestris in all treatments was below the Chinese safety standard. When sodium silicate and Ca-Mg-P fertilizer were added to the soil, the additional foliar application of Se and Si could in some cases help further reduce the concentrations of As and Cd in the leaves of B...
April 12, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28410966/vincristine-induced-peripheral-neuropathic-pain-and-expression-of-transient-receptor-potential-vanilloid-1-in-rat
#9
Terumasa Chiba, Yusuke Oka, Hiroya Sashida, Toshie Kanbe, Kenji Abe, Iku Utsunomiya, Kyoji Taguchi
The clinical anti-cancer efficacy of vincristine is limited by the development of dose-dependent peripheral neuropathy. Up-regulation of transient receptor potential vanilloid 1 (TRPV1) is correlated with peripheral neuropathy following anti-cancer drug treatment. To analyze the contribution of TRPV1 to the development of vincristine-induced mechanical allodynia/hyperalgesia, TRPV1 expression in the rat dorsal root ganglion (DRG) was analyzed after vincristine treatment. Mechanical allodynia/hyperalgesia was tested with von Frey filaments 14 days after intraperitoneal administration of 0...
March 30, 2017: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/28393209/puerarin-attenuates-myocardial-hypoxia-reoxygenation-injury-by-inhibiting-autophagy-via-the-akt-signaling-pathway
#10
Huixiong Tang, Xudong Song, Yuanna Ling, Xianbao Wang, Pingzhen Yang, Tao Luo, Aihua Chen
Puerarin (Pur), which is the major bioactive ingredient extracted from the root of Pueraria lobata (Willd.) Ohwi, has been demonstrated to relieve myocardial ischemia/reperfusion (I/R) injury. Macroautophagy, or autophagy, is an evolutionarily conserved cellular catabolic mechanism that is involved in myocardial I/R injury. The present study evaluated the involvement of autophagy in the protective mechanisms of Pur during myocardial hypoxia/reoxygenation (H/R). The results revealed that Pur and 3‑methyladenine pretreatment exerted a cardioprotective effect against H/R‑induced cell viability loss...
April 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28388364/piezo2-expression-in-mechanosensitive-dental-primary-afferent-neurons
#11
J Won, H Vang, P R Lee, Y H Kim, H W Kim, Y Kang, S B Oh
Mechanosensitive ion channels have been suggested to be expressed in dental primary afferent (DPA) neurons to transduce the movement of dentinal fluid since the proposal of hydrodynamic theory. Piezo2, a mechanosensitive, rapidly inactivating (RI) ion channel, has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. Here, we examined the expression of Piezo2 in DPA neurons by in situ hybridization, single-cell reverse transcriptase polymerase chain reaction, and whole-cell patch-clamp recordings...
April 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28385699/treating-sickle-cell-disease-by-targeting-hbs-polymerization
#12
William A Eaton, H Franklin Bunn
Although the root cause of sickle cell disease is the polymerization of HbS to form fibers that make red cells less flexible, most drugs currently in clinical trials are targeting the downstream sequelae of this primary event. Less attention has been devoted toward investigation of the multiple ways in which fiber formation can be inhibited. In this Perspective, we describe the molecular rationale for 5 distinct approaches to inhibit polymerization and also discuss progress with the few anti-polymerization drugs currently in clinical trials...
April 6, 2017: Blood
https://www.readbyqxmd.com/read/28376712/foliar-applied-ethephon-enhances-the-content-of-anthocyanin-of-black-carrot-roots-daucus-carota-ssp-sativus-var-atrorubens-alef
#13
Gregorio Barba-Espín, Stephan Glied, Christoph Crocoll, Tsaneta Dzhanfezova, Bjarne Joernsgaard, Finn Okkels, Henrik Lütken, Renate Müller
BACKGROUND: Black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) constitute a valuable source of anthocyanins, which are used as natural red, blue and purple food colourants. Anthocyanins and phenolic compounds are specialised metabolites, accumulation of which often requires elicitors, which act as molecular signals in plant stress responses. In the present study, ethephon, an ethylene-generating compound was explored as enhancer of anthocyanin and phenolic contents during growth of 'Deep Purple' black carrots...
April 4, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28375005/expanding-the-chemical-repertoire-of-the-endophyte-streptomyces-albospinus-rle7-reveals-amphotericin-b-as-an-inducer-of-a-fungal-phenotype
#14
Fernanda Oliveira Chagas, Andrés Mauricio Caraballo-Rodríguez, Pieter C Dorrestein, Mônica Tallarico Pupo
During an investigation of the chemistry of the endophytic actinobacterium Streptomyces albospinus RLe7, which was isolated from the roots of the Brazilian medicinal plant Lychnophora ericoides, three new natural products, (2R*,4S*)-2-((1'S*)-hydroxy-4'-methylpentyl)-4-(hydroxymethyl)butanolide (1), (3R*,4S*,5R*,6S*)-tetrahydro-4-hydroxy-3,5,6-trimethyl-2-pyranone (2), and 1-O-(phenylacetyl)glycerol (3), together with known secondary metabolites (S)-4-benzyl-3-oxo-3,4-dihydro-1H-pyrrolo[2,1-c][1,4]oxazine-6-carbaldehyde (4), (S)-4-isobutyl-3-oxo-3,4-dihydro-1H-pyrrolo[2,1-c][1,4]oxazine-6-carbaldehyde (5), and the diketopiperazines cyclo(l-Tyr-l-Pro) (6) and cyclo(l-Val-l-Pro) (7), were isolated...
April 4, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28370629/a-group-of-grapevine-myba-transcription-factors-located-in-chromosome-14-control-anthocyanin-synthesis-in-vegetative-organs-with-different-specificities-compared-to-the-berry-color-locus
#15
José Tomás Matus, Erika Cavallini, Rodrigo Loyola, Janine Höll, Laura Finezzo, Silvia Dal Santo, Sandrine Vialet, Mauro Commisso, Federica Roman, Andrea Schubert, José Antonio Alcalde, Jochen Bogs, Agnès Ageorges, Giovanni Battista Tornielli, Patricio Arce-Johnson
Grapevine organs accumulate anthocyanins in a cultivar-specific and environmentally induced manner. The MYBA1-A2 genes within the berry color locus in chromosome 2 represent the major genetic determinants of fruit color. The simultaneous occurrence of transposon insertions and point mutations in these genes is responsible for most white-skinned phenotypes. However, red pigmentation found in vegetative organs suggests the presence of additional regulators. This work describes a genomic region of chromosome 14 containing three closely related R2R3-MYB genes, named MYBA5, MYBA6 and MYBA7...
April 2, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28367156/plant-growth-and-photosynthetic-characteristics-of-mesembryanthemum-crystallinum-grown-aeroponically-under-different-blue-and-red-leds
#16
Jie He, Lin Qin, Emma L C Chong, Tsui-Wei Choong, Sing Kong Lee
Mesembryanthemum crystallinum is a succulent, facultative crassulacean acid metabolism (CAM) plant. Plant growth and photosynthetic characteristics were studied when M. crystallinum plants were grown indoor under light emitting diodes (LED)-lighting with adequate water supply. Plants were cultured aeroponically for a 16-h photoperiod at an equal photosynthetic photon flux density of 350 μmol m(-2) s(-1) under different red:blue LED ratios: (1) 100:0 (0B); (2) 90:10 (10B); (3) 80:20 (20B); (4) 70:30 (30B); (5) 50:50 (50B); and (6)100:0 (100B)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28337797/standardized-sophora-pachycarpa-root-extract-enhances-osteogenic-differentiation-in-adipose-derived-human-mesenchymal-stem-cells
#17
Samaneh Mollazadeh, Vajiheh Neshati, Bibi Sedigheh Fazly Bazzaz, Mehrdad Iranshahi, Majid Mojarrad, Hojjat Naderi-Meshkin, Mohammad Amin Kerachian
Bone defect is an important topic in public health. Novel therapies are based on osteogenic induction by natural antiosteoporotic compounds including plant-derived estrogens. In the current study, the osteogenic potential of Sophora pachycarpa root extract (SPRE) was explored on human adipose-derived mesenchymal stem cells. Herein, adipose-derived mesenchymal stem cells were osteoinducted in the presence of increased concentrations of the extract for 21 days. Then, cell viability was evaluated by MTT assay, and the differentiated cells were stained by Alizarin Red S for calcium deposition and subjected to alkaline phosphatase (ALP) assay for enzymatic activity...
March 24, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28335679/puerarin-attenuates-renal-fibrosis-by-reducing-oxidative-stress-induced-epithelial-cell-apoptosis-via-mapk-signal-pathways-in-vivo-and-in-vitro
#18
Xiangjun Zhou, Chen Bai, Xinbo Sun, Xiaoxin Gong, Yong Yang, Congbo Chen, Guang Shan, Qisheng Yao
Puerarin (PR) is an isoflavonoid isolated from the root of the plant Pueraria lobata and has been widely used in traditional Chinese herbal medicine for the treatment of various diseases. Oxidative stress and epithelial cell apoptosis play important roles in the renal fibrotic process. The present study aimed to determine whether or not PR inhibits renal fibrosis by reducing oxidative stress induced-epithelial cell apoptosis. In vivo, unilateral ureteral obstruction (UUO) induced renal fibrosis, and epithelial cell apoptosis...
November 2017: Renal Failure
https://www.readbyqxmd.com/read/28332191/combined-application-of-er-yag-and-nd-yag-lasers-in-treatment-of-chronic-periodontitis-a-split-mouth-single-blind-randomized-controlled-trial
#19
M Sağlam, S Köseoğlu, I Taşdemir, H Erbak Yılmaz, L Savran, R Sütçü
BACKGROUND: The aim of the present study was to compare the effectiveness of combined Er:YAG and Nd:YAG laser therapy to that of scaling and root planing with hand instruments in non-surgical treatment of chronic periodontitis. MATERIAL AND METHODS: Twenty-five systemically healthy patients with chronic periodontitis were selected for this study. The quadrants were randomly allocated in a split-mouth design to either combined Er:YAG (160 mJ/pulse, 10 Hz) and Nd:YAG laser (100 mJ/pulse, 20 Hz) therapy (test group) or scaling and root planing alone (control group)...
March 23, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28330554/qualitative-and-quantitative-modifications-of-root-mitochondria-during-senescence-of-above-ground-parts-of-arabidopis-thaliana
#20
Diego Darío Fanello, Carlos Guillermo Bartoli, Juan José Guiamet
This work studied modifications experienced by root mitochondria during whole plant senescence or under light deprivation, using Arabidopsis thaliana plants with YFP tagged to mitochondria. During post-bolting development, root respiratory activity started to decline after aboveground organs (i.e., rosette leaves) had senesced. This suggests that carbohydrate starvation may induce root senescence. Similarly, darkening the whole plant induced a decrease in respiration of roots. This was partially due to a decrease in the number of total mitochondria (YFP-labelled mitochondria) and most probably to a decrease in the quantity of mitochondria with a developed inner membrane potential (ΔΨm, i...
May 2017: Plant Science: An International Journal of Experimental Plant Biology
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