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https://www.readbyqxmd.com/read/28747886/in-vitro-thermal-maps-to-characterize-human-dental-enamel-and-dentin
#1
Paula Lancaster, David Brettle, Fiona Carmichael, Val Clerehugh
The crown of a human tooth has an outer layer of highly-mineralized tissue called enamel, beneath which is dentin, a less-mineralized tissue which forms the bulk of the tooth-crown and root. The composition and structure of enamel and dentin are different, resulting in different thermal properties. This gives an opportunity to characterize enamel and dentin from their thermal properties and to visually present the findings as a thermal map. The thermal properties of demineralized enamel and dentin may also be sufficiently different from sound tissue to be seen on a thermal map, underpinning future thermal assessment of caries...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28742069/nematode-grazing-promotes-bacterial-community-dynamics-in-soil-at-the-aggregate-level
#2
Yuji Jiang, Manqiang Liu, Jiabao Zhang, Yan Chen, Xiaoyun Chen, Lijun Chen, Huixin Li, Xue-Xian Zhang, Bo Sun
Nematode predation has important roles in determining bacterial community composition and dynamics, but the extent of the effects remains largely rudimentary, particularly in natural environment settings. Here, we investigated the complex microbial-microfaunal interactions in the rhizosphere of maize grown in red soils, which were derived from four long-term fertilization regimes. Root-free rhizosphere soil samples were separated into three aggregate fractions whereby the abundance and community composition were examined for nematode and total bacterial communities...
July 25, 2017: ISME Journal
https://www.readbyqxmd.com/read/28732980/synthesis-of-amine-modified-zeolitic-imidazolate-framework-8-ultrasound-assisted-dye-removal-and-modeling
#3
Jafar Abdi, Manouchehr Vossoughi, Niyaz Mohammad Mahmoodi, Iran Alemzadeh
The present research is focused on the ultrasound assisted adsorption of Acid blue 92 (AB92) and Direct red 80 (DR80) as anionic dyes in single and binary systems onto zeolitic imidazolate framework (ZIF-8) functionalized with 3-Aminopropyltrimethoxysilane (APTES). Different techniques such as Fourier transform infrared (FTIR), scanning electron microscope (SEM), field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) and thermogravimetric analyses (TGA) were used to characterize the prepared adsorbent...
November 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28729876/genetic-architecture-of-flooding-tolerance-in-the-dry-bean-middle-american-diversity-panel
#4
Ali Soltani, Samira MafiMoghaddam, Katelynn Walter, Daniel Restrepo-Montoya, Sujan Mamidi, Stephan Schroder, Rian Lee, Phillip E McClean, Juan M Osorno
Flooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for further improvement. However, challenging phenotyping methods hinder a large-scale genetic study of flooding tolerance in common bean and other economically important crops. A greenhouse phenotyping protocol was developed to evaluate the flooding conditions at early stages...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28709892/liuwei-dihuang-soft-capsules-attenuates-endothelial-cell-apoptosis-to-prevent-atherosclerosis-through-gpr30-mediated-regulation-in-ovariectomized-apoe-deficient-mice
#5
Yi Jing, Danfeng Cai, Qi Chen, Qingping Xiong, Tianhui Hu, Yuan Yao, Chao Lin, Xin Sun, Ying Lu, Xueyun Kong, Xiang Wu, Yu Li, Huimin Bian
ETHNOPHARMACOLOGICAL RELEVANCE: Liuwei Dihuang (LWDH), a classical traditional Chinese medicine prescription, has been widely used to prevent and to treat various diseases with symptoms of 'Kidney-Yin' deficiency syndrome for over 1000 years in China. It is commonly used to treat functional decline associated with senile disease and menopausal syndrome, especially memory decline, insomnia, diabetes and osteoporosis. Modern experimental pharmacological studies indicated that the mechanism of LWDH treatment of menopausal syndrome may be associated with enhanced estrogenic effects...
July 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28709148/impact-of-chronic-methamphetamine-treatment-on-the-atherosclerosis-formation-in-apoe-mice-fed-a-high-cholesterol-diet
#6
Pengfei Zhu, Lun Li, Bo Gao, Mingjing Zhang, Yuting Wang, Ye Gu, Liqun Hu
BACKGROUND: We previously reported that methamphetamine could promote atherosclerosis (AS) in ApoE-/- mice fed normal chow. We herein observed the impact of methamphetamine on AS in ApoE-/- mice fed a high cholesterol diet and explored the potential mechanisms.Results and Materials and Methods: Male ApoE-/- mice fed a high cholesterol diet were treated with saline (NS, n = 5) or methamphetamine [8 mg/kg/day (M8, n = 6) through intraperitoneal injection] for 24 weeks. Afterwards, the percentage area of atheromatous plaque in aortic root (44...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28706181/effects-of-an-enriched-extract-of-paeoniflorin-a-monoterpene-glycoside-used-in-chinese-herbal-medicine-on-cholesterol-metabolism-in-a-hyperlipidemic-rat-model
#7
Huiming Hu, Qiaoqiao Zhu, Jie Su, Yajun Wu, Yanchen Zhu, Yin Wang, Hui Fang, Minxia Pang, Bo Li, Suhong Chen, Guiyuan Lv
BACKGROUND Paeoniflorin is a monoterpene glycoside extracted from the roots of Paeonia lactiflora and is used in Chinese herbal medicine to treat hyperlipidemia. The aim of this study was to evaluate the effects of an enriched extract of paeoniflorin on cholesterol levels, hemodynamics, and oxidative stress in a hyperlipidemic rat model. MATERIAL AND METHODS Male Sprague-Dawley rats were fed high-cholesterol diets and treated with three different doses of paeoniflorin for 12 weeks. The effects of paeoniflorin treatment were assessed on cholesterol levels, cholesterol metabolism, red blood cell vascular flow using hemorheology, antioxidant enzymes, and expression of the rate-limiting enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutharyl-coenzyme A reductase (HMG-CoAR)...
July 14, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28701878/in-situ-analysis-of-chemical-components-induced-by-steaming-between-fresh-ginseng-steamed-ginseng-and-red-ginseng
#8
Gyo In, Nam-Geun Ahn, Bong-Seok Bae, Myoung-Woo Lee, Hee-Won Park, Kyoung Hwa Jang, Byung-Goo Cho, Chang Kyun Han, Chae Kyu Park, Yi-Seong Kwak
BACKGROUND: The chemical constituents of Panax ginseng are changed by processing methods such as steaming or sun drying. In the present study, the chemical change of Panax ginseng induced by steaming was monitored in situ. METHODS: Samples were separated from the same ginseng root by incision during the steaming process, for in situ monitoring. Sampling was sequentially performed in three stages; FG (fresh ginseng) → SG (steamed ginseng) → RG (red ginseng) and 60 samples were prepared and freeze dried...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28701871/changes-in-element-accumulation-phenolic-metabolism-and-antioxidative-enzyme-activities-in-the-red-skin-roots-of-panax-ginseng
#9
Ying Zhou, Zhenming Yang, Lingling Gao, Wen Liu, Rongkun Liu, Junting Zhao, Jiangfeng You
BACKGROUND: Red-skin root disease has seriously decreased the quality and production of Panax ginseng (ginseng). METHODS: To explore the disease's origin, comparative analysis was performed in different parts of the plant, particularly the epidermis, cortex, and/or fibrous roots of 5-yr-old healthy and diseased red-skin ginseng. The inorganic element composition, phenolic compound concentration, reactive oxidation system, antioxidant concentrations such as ascorbate and glutathione, activities of enzymes related to phenolic metabolism and oxidation, and antioxidative system particularly the ascorbate-glutathione cycle were examined using conventional methods...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28698804/the-knee-meniscus-management-of-traumatic-tears-and-degenerative-lesions
#10
Philippe Beaufils, Roland Becker, Sebastian Kopf, Ollivier Matthieu, Nicolas Pujol
Meniscectomy is one of the most popular orthopaedic procedures, but long-term results are not entirely satisfactory and the concept of meniscal preservation has therefore progressed over the years. However, the meniscectomy rate remains too high even though robust scientific publications indicate the value of meniscal repair or non-removal in traumatic tears and non-operative treatment rather than meniscectomy in degenerative meniscal lesionsIn traumatic tears, the first-line choice is repair or non-removal...
May 2017: EFORT open reviews
https://www.readbyqxmd.com/read/28688217/yes-associated-protein-1-promotes-the-differentiation-and-mineralization-of-cementoblast
#11
Beining Yang, Hualing Sun, Fangfang Song, Yanru Wu, Jiawei Wang
Yes-associated protein 1 (YAP1) transcriptional coactivator is a mediator of mechanosensitive signaling. Cementum, which covers the tooth root surface, continuously senses external mechanical stimulation. Cementoblasts are responsible for the mineralization and maturation of the cementum. However, the effect of YAP1 on cementoblast differentiation remains largely unknown. In this study, we initially demonstrated that YAP1 overexpression enhanced the mineralization ability of cementoblasts. YAP1 upregulated the mRNA and protein expression of several cementogenesis markers, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and dentin matrix acidic phosphoprotein 1 (DMP1)...
July 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28682552/-effects-of-oral-interventions-on-carotid-artery-in-rats-with-chronic-periodontitis-for-the-detection-of-porphyromonas-gingivalis-and-the-expression-of-c-reactive-protein
#12
Ren Xiuyun, Wang Chong, Liu Xin, Li Hao, Ma Qianhui, Lin Mu, Shi Xuexue, Gao Jinhua
OBJECTIVE: This study aimed to establish a SD rat model of chronic periodontitis (CP) merged with hyperlipidemia (HL), perform periodontal treatment, detect the expression of partial C-reactive protein (CRP) and Porphyromonas gingivalis (P. gingivalis) in the rat carotid artery, and explore the relationship between periodontitis and atherosclerosis. METHODS: SD rats were randomly divided into three groups: control group (A), HL group (B), and CP+HL group (C). Group C rats were divided into natural process group (C1), scaling and root planning group (C2), and tooth extraction group (C3)...
April 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28678972/the-role-of-osterix-protein-in-the-pathogenesis-of-peripheral-ossifying-fibroma
#13
Vivian Narana Ribeiro El Achkar, Raphaella da Silveira Medeiros, Fernanda Gargano Longue, Ana Lia Anbinder, Estela Kaminagakura
Peripheral ossifying fibroma (POF) is a reactive lesion of oral tissues, associated with local factors such as trauma or presence of dental biofilm. POF treatment consists of curettage of the lesion combined with root scaling of adjacent teeth and/or removal of other sources of irritants. This study aimed to analyze the clinical and pathological features of POF and to investigate the immunoexpression of Osterix and STRO-1 proteins. Data such as age, gender, and size were obtained from 30 cases of POF. Microscopic features were assessed by conventional light microscopy using hematoxylin-eosin staining and immunohistochemical markers, and by polarized light microscopy using Picrosirius red staining...
July 3, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28669594/potential-to-induce-dentinal-cracks-during-retreatment-procedures-of-teeth-treated-with-russian-red-an-ex-vivo-study
#14
Eglė Nedzinskienė, Vytautė Pečiulienė, Jolanta Aleksejūnienė, Rasmutė Manelienė, Saulius Drukteinis, Audronė Jakaitienė
OBJECTIVE: The aim of this study was to compare the impact of treatment procedures on roots previously treated with resorcinol-formaldehyde resin and analyze the effectiveness of dye and magnification for the detection of dentin cracks. MATERIALS AND METHODS: Distal roots of 80 permanent first mandibular molars with a single canal were sectioned at 3mm and 9mm from the anatomical apex. Two groups were formed according to the method used for root canal penetration: group 1 (K-file and Pro Taper instruments) and group 2 (Ultrasound with Pro Ultra and Pro Taper files)...
June 7, 2017: Medicina
https://www.readbyqxmd.com/read/28667265/transcriptome-analysis-explores-genes-related-to-shikonin-biosynthesis-in-lithospermeae-plants-and-provides-insights-into-boraginales-evolutionary-history
#15
Feng-Yao Wu, Cheng-Yi Tang, Yu-Min Guo, Zhuo-Wu Bian, Jiang-Yan Fu, Gui-Hua Lu, Jin-Liang Qi, Yan-Jun Pang, Yong-Hua Yang
Shikonin and its derivatives extracted from Lithospermeae plants' red roots have current applications in food and pharmaceutical industries. Previous studies have cloned some genes related to shikonin biosynthesis. However, most genes related to shikonin biosynthesis remain unclear, because the lack of the genome/transcriptome of the Lithospermeae plants. Therefore, in order to provide a new understanding of shikonin biosynthesis, we obtained transcriptome data and unigenes expression profiles in three shikonin-producing Lithospermeae plants, i...
June 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28665292/hydrodynamics-of-mangrove-type-root-models-the-effect-of-porosity-spacing-ratio-and-flexibility-i-i-i-i
#16
Amirkhosro Kazemi, Keith Van de Riet, Oscar M Curet
Mangrove trees play a prominent role in coastal tropic and subtropical regions, providing habitats for many organisms and protecting shorelines against high energy flows. In particular, the species <i>Rhizophora mang</i>le (red mangrove) exhibits complex cluster roots interacting with different hydrological flow conditions. To better understand the resilience of mangrove trees, we modeled the roots as a collection of cylinders with a circular pattern subject to unidirectional flow. We investigated the effect of porosity and spacing ratio between roots by varying both the diameter of the patch, <i>D</i>, and inset cylinders,<i> d</i>...
June 30, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28664334/new-approaches-for-extraction-and-determination-of-betaine-from-beta-vulgaris-samples-by-hydrophilic-interaction-liquid-chromatography-tandem-mass-spectrometry
#17
Luca Rivoira, Sylwia Studzińska, Malgorzata Szultka-Młyńska, Maria Concetta Bruzzoniti, Bogusław Buszewski
Betaine is one of most studied biologically active compounds, due its role in the main biological processes. Although it may be found in several plants and roots, such as the Beta vulgaris family, present in typical diets, just a few analytical methods have been developed for its extraction from roots. A new, quick and effective procedure for the isolation and determination of betaine from two different varieties of B. vulgaris (red and gold) is presented. For betaine extraction, an accelerated solvent extraction (ASE) was coupled with solid-phase extraction...
June 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28659970/effects-of-genotype-by-environment-interaction-on-genetic-gain-and-genetic-parameter-estimates-in-red-tilapia-oreochromis-spp
#18
Nguyen H Nguyen, Azhar Hamzah, Ngo P Thoa
The extent to which genetic gain achieved from selection programs under strictly controlled environments in the nucleus that can be expressed in commercial production systems is not well-documented in aquaculture species. The main aim of this paper was to assess the effects of genotype by environment interaction on genetic response and genetic parameters for four body traits (harvest weight, standard length, body depth, body width) and survival in Red tilapia (Oreochromis spp.). The growth and survival data were recorded on 19,916 individual fish from a pedigreed population undergoing three generations of selection for increased harvest weight in earthen ponds from 2010 to 2012 at the Aquaculture Extension Center, Department of Fisheries, Jitra in Kedah, Malaysia...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28658312/red-rot-resistant-transgenic-sugarcane-developed-through-expression-of-%C3%AE-1-3-glucanase-gene
#19
Shivani Nayyar, Bipen Kumar Sharma, Ajinder Kaur, Anu Kalia, Gulzar Singh Sanghera, Karanjit Singh Thind, Inderjit Singh Yadav, Jagdeep Singh Sandhu
Sugarcane (Saccharum spp.) is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery. We report development of red rot resistant transgenic sugarcane through expression of β-1,3-glucanase gene from Trichoderma spp. The transgene integration and its expression were confirmed by quantitative reverse transcription-PCR in first clonal generation raised from T0 plants revealing up to 4...
2017: PloS One
https://www.readbyqxmd.com/read/28654160/pontamine-fast-scarlet-4b-bifluorescence-and-measurements-of-cellulose-microfibril-angles
#20
J Thomas, N A Idris, D A Collings
Pontamine fast scarlet 4B is a red paper and textiles dye that has recently been introduced as a fluorescent probe for plant cell walls. Pontamine exhibits bifluorescence, or fluorescence dependent on the polarization of the excitation light: Because cellulose is aligned within the cell wall, pontamine-labelled cell walls exhibit variable fluorescence as the excitation polarization is modulated. Thus, bifluorescence measurements require polarized excitation that can be directly or indirectly modulated. In our confocal microscopy observations of various cellulose samples labelled with pontamine, we modulated excitation polarization either through sample rotation or by the confocal's scanfield rotation function...
June 27, 2017: Journal of Microscopy
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