Mikala M Zelows, Corissa Cady, Nikitha Dharanipragada, Anna E Mead, Zachary A Kipp, Evelyn A Bates, Venkateshwari Varadharajan, Rakhee Banerjee, Se-Hyung Park, Nathan R Shelman, Harrison A Clarke, Tara R Hawkinson, Terrymar Medina, Ramon C Sun, Todd A Lydic, Terry D Hinds, J Mark Brown, Samir Softic, Gregory A Graf, Robert N Helsley
BACKGROUND AND AIMS: Genome and epigenome wide association studies identified variants in carnitine palmitoyltransferase 1a (CPT1a) that associate with lipid traits. The goal of this study was to determine the role of liver-specific CPT1a on hepatic lipid metabolism. APPROACH AND RESULTS: Male and female liver-specific knockout (LKO) and littermate controls were placed on a low-fat or high-fat diet (60% kcal fat) for 15 weeks. Mice were necropsied after a 16 hour fast, and tissues were collected for lipidomics, matrix-assisted laser desorption ionization mass spectrometry imaging, kinome analysis, RNA-sequencing, and protein expression by immunoblotting...
October 3, 2023: Molecular Metabolism