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rpoB mutation

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https://www.readbyqxmd.com/read/29031637/molecular-epidemiology-of-multi-and-extensively-drug-resistant-mycobacterium-tuberculosis-in-ireland-2001-2014
#1
E Roycroft, R F O'Toole, M M Fitzgibbon, L Montgomery, M O'Meara, P Downes, S Jackson, J O'Donnell, I F Laurenson, A M McLaughlin, J Keane, T R Rogers
OBJECTIVES: The primary objective of this work was to examine the acquisition and spread of multi-drug resistant (MDR) tuberculosis (TB) in Ireland. METHODS: All available Mycobacterium tuberculosis complex (MTBC) isolates (n=42), from MDR-TB cases diagnosed in Ireland between 2001 and 2014, were analysed using phenotypic drug-susceptibility testing, Mycobacterial-Interspersed-Repetitive-Units Variable-Number Tandem-Repeat (MIRU-VNTR) genotyping, and whole-genome sequencing (WGS)...
October 11, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28993300/analysis-of-isoniazid-and-rifampicin-resistance-in-mycobacterium-tuberculosis-isolates-in-morocco-using-genotype-mtbdrplus-assay
#2
Wifak Ennassiri, Sanae Jaouhari, Radia Sabouni, Wafa Cherki, Reda Charof, Abdelkarim Filali-Maltouf, Ouafae Lahlou
OBJECTIVES: The number of multidrug-resistant tuberculosis (MDR-TB) cases is rising worldwide. The present investigation aims to evaluate, using the GenoType(®) MTBDRplus, the most common mutations associated with rifampicin (RMP) and isoniazid (INH) resistance within the resistant strains in Morocco. METHODS: 319M. tuberculosis isolates referred to the National Tuberculosis Reference Laboratory, between 2013 and 2015, were subjected to GenoType(®) MTBDRplus for detecting M...
October 6, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28961910/adaptive-mutations-in-rna-polymerase-and-the-transcriptional-terminator-rho-have-similar-effects-on-escherichia-coli-gene-expression
#3
Andrea González-González, Shaun M Hug, Alejandra Rodríguez-Verdugo, Jagdish Suresh Patel, Brandon S Gaut
Modifications to transcriptional regulators play a major role in adaptation. Here we compared the effects of multiple beneficial mutations within and between Escherichia coli rpoB, the gene encoding the RNA polymerase β subunit, and rho, which encodes a transcriptional terminator. These two genes have harbored adaptive mutations in numerous E. coli evolution experiments but particularly in our previous large-scale thermal stress experiment, where the two genes characterized alternative adaptive pathways. To compare the effects of beneficial mutations, we engineered four advantageous mutations into each of the two genes and measured their effects on fitness, growth, gene expression and transcriptional termination at 42...
August 9, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28959248/rifabutin-resistance-associated-with-double-mutations-in-rpob-gene-in-mycobacterium-tuberculosis-isolates
#4
Wei Jing, Yu Pang, Zhaojing Zong, Jing Wang, Ru Guo, Fengmin Huo, Guanglu Jiang, Yifeng Ma, Hairong Huang, Naihui Chu
The objective of this study was to investigate the cross-resistance between rifampin (RIF) and rifabutin (RFB) among clinical Mycobacterium tuberculosis (MTB) isolates, and the correlations between specific rpoB mutations and the minimum inhibitory concentrations (MICs) of RIF and RFB. A total of 256 RIF-resistant isolates were included from the National Tuberculosis Clinical Laboratory in China. The MICs of MTB isolates against RIF and RFB were determined by using a microplate alamarBlue assay. In addition, all the MTB isolates were sequenced for mutations in rpoB gene...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28931561/genetic-mimetics-of-mycobacterium-tuberculosis-and-methicillin-resistant-staphylococcus-aureus-as-verification-standards-for-molecular-diagnostics
#5
Edith Erika Machowski, Bavesh Davandra Kana
Molecular diagnostics have revolutionized the management of health care through enhanced detection of disease or infection and effective engagement into care. In recognition of this, the World Health Organization approved the rollout out of nucleic acid amplification technologies for identification of Mycobacterium tuberculosis using platforms such as GeneXpert MTB/RIF, the GenoType MTBDRplus Line Probe Assay and more recently, GeneXpert MTB/RIF Ultra. These assays can simultaneously detect tuberculosis infection and assess rifampicin resistance...
September 20, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28930567/molecular-mechanisms-of-chlamydia-trachomatis-resistance-to-antimicrobial-drugs
#6
Tomislav Mestrovic, Suncanica Ljubin-Sternak
Chlamydia trachomatis (C. trachomatis) is a leading cause of bacterial sexually transmitted infections in developed and undeveloped countries, and therefore a global public health issue. In an era of increasing bacterial resistance to antibiotics, resistance has been an exceedingly rare phenomenon in C. trachomatis; however, clinical treatment failures attributed to multidrug-resistant C. trachomatis strains have been described on several occasions. Cell culture systems using McCoy cells and subsequent immunofluorescent staining are still the most common methodology used for antimicrobial susceptibility testing, but the presence of resistance markers should be appraised by further genetic analysis...
January 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28904673/mutations-in-rpob-and-katg-genes-of-multidrug-resistant-mycobacterium-tuberculosis-undetectable-using-genotyping-diagnostic-methods
#7
Faustinos Tatenda Takawira, Racheal Shamiso Dube Mandishora, Zephaniah Dhlamini, Ellen Munemo, Babill Stray-Pedersen
INTRODUCTION: Tuberculosis remains the leading causes of death worldwide with frequencies of mutations in rifampicin and isoniazid resistant Mycobacterium tuberculosis isolates varying according to geographical location. There is limited information in Zimbabwe on specific antibiotic resistance gene mutation patterns in MTB and hence, increased rate of discordant results and mortality due to inappropriate antibiotic prescriptions. The rpoB and katG genes molecular markers are used for detecting rifampicin and isoniazid resistance respectively...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28900077/-the-spectrum-of-mutations-in-genes-associated-with-resistance-to-rifampicin-isoniazid-and-fluoroquinolones-in-the-clinical-strains-of-m-tuberculosis-reflects-the-transmissibility-of-mutant-clones
#8
A Ergeshov, S N Andreevskaya, E E Larionova, T G Smirnova, L N Chernousova
To study the transmissibility of drug resistant mutant clones, M. tuberculosis samples were isolated from the patients of the clinical department and the polyclinic of the Central TB Research Institute (n = 1455) for 2011-2014. A number of clones were phenotypically resistant to rifampicin (n = 829), isoniazid (n = 968), and fluoroquinolones (n = 220). We have detected 21 resistance-associated variants in eight codons of rpoB, six variants in three codons of katG, three variants in two positions of inhA, four variants in four positions of ahpC, and nine variants in five codons of gyrA, which were represented in the analyzed samples with varied frequencies...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28884143/use-of-genotype-mtbdrplus-assay-for-diagnosis-of-multidrug-resistant-tuberculosis-in-nepal
#9
Elina Maharjan, Narayan Dutt Pant, Sanjeev Neupane, Jyoti Amatya, Bhawana Shrestha
The main aims of this study were to study the patterns of mutations in rpoB, katG, and inhA genes in Mycobacterium tuberculosis strains isolated from patients from Nepal and to evaluate the performance of genotype MTBDRplus assay, taking conventional drug susceptibility testing as gold standard for diagnosis of MDR-TB. A total of 69 Mycobacterium tuberculosis strains isolated from 73 smear positive sputum samples from patients suspected of suffering from multidrug-resistant tuberculosis were used in our study...
2017: International Scholarly Research Notices
https://www.readbyqxmd.com/read/28879159/molecular-characterisation-of-rifampicin-resistant-mycobacterium-tuberculosis-strains-from-malawi
#10
Tarsizio Chikaonda, Irene Ketseoglou, Nelson Nguluwe, Robert Krysiak, Isaac Thengolose, Felix Nyakwawa, Nora E Rosenberg, Christopher Stanley, James Mpunga, Irving F Hoffman, Maria A Papathanasopoulos, Mina Hosseinipour, Lesley Scott, Wendy Stevens
BACKGROUND: Availability and access to the detection of resistance to anti-tuberculosis drugs remains a significant challenge in Malawi due to limited diagnostic services. The Xpert(®) MTB/RIF can detect Mycobacterium tuberculosis and resistance to rifampicin in a single, rapid assay. Rifampicin-resistant M. tuberculosis has not been well studied in Malawi. OBJECTIVES: We aimed to determine mutations in the rifampicin resistance determining region (RRDR) of the rpoB gene of M...
2017: African Journal of Laboratory Medicine
https://www.readbyqxmd.com/read/28851844/the-new-xpert-mtb-rif-ultra-improving-detection-of-mycobacterium-tuberculosis-and-resistance-to-rifampin-in-an-assay-suitable-for-point-of-care-testing
#11
Soumitesh Chakravorty, Ann Marie Simmons, Mazhgan Rowneki, Heta Parmar, Yuan Cao, Jamie Ryan, Padmapriya P Banada, Srinidhi Deshpande, Shubhada Shenai, Alexander Gall, Jennifer Glass, Barry Krieswirth, Samuel G Schumacher, Pamela Nabeta, Nestani Tukvadze, Camilla Rodrigues, Alena Skrahina, Elisa Tagliani, Daniela M Cirillo, Amy Davidow, Claudia M Denkinger, David Persing, Robert Kwiatkowski, Martin Jones, David Alland
The Xpert MTB/RIF assay (Xpert) is a rapid test for tuberculosis (TB) and rifampin resistance (RIF-R) suitable for point-of-care testing. However, it has decreased sensitivity in smear-negative sputum, and false identification of RIF-R occasionally occurs. We developed the Xpert MTB/RIF Ultra assay (Ultra) to improve performance. Ultra and Xpert limits of detection (LOD), dynamic ranges, and RIF-R rpoB mutation detection were tested on Mycobacterium tuberculosis DNA or sputum samples spiked with known numbers of M...
August 29, 2017: MBio
https://www.readbyqxmd.com/read/28840388/molecular-screening-of-multidrug-resistance-tuberculosis-by-a-designated-public-health-laboratory-in-taiwan
#12
H-C Lin, C-L Perng, Y-W Lai, F-G Lin, C-J Chiang, H-A Lin, R Jou, T-S Chiueh
This manuscript describes our experience in early identifying MDR-TB cases in high-risk populations by setting up a single-referral molecular diagnosis laboratory in Taiwan. Taiwan Centers for Disease Control designated a single-referral laboratory to provide the GenoType MTBDRplus test for screening high-risk MDR-TB populations nationwide in 2012-2015. A total of 5,838 sputum specimens from 3,308 patients were tested within 3 days turnaround time. Compared with the conventional culture and drug susceptibility testing, the overall performance of the GenoType MTBDRplus test for detecting TB infection showed accuracy of 70...
August 24, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28817723/sequence-analysis-of-the-rifampicin-resistance-determining-region-rrdr-of-rpob-gene-in-multidrug-resistance-confirmed-and-newly-diagnosed-tuberculosis-patients-of-punjab-pakistan
#13
Salma Hameed, Kartyk Moganeradj, Nasir Mahmood, Timothy D McHugh, Muhammad Nawaz Chaudhry, Catherine Arnold
Molecular screening of new patients suspected for TB could help in the effective control of TB in Pakistan as it is a high TB burden country. It will be informative to understand the prevalence of multi drug resistance for a better drug regimen management in this geographical area. The Rifampicin resistance determining region (RRDR) sequencing was used to identify mutations associated with drug resistance in DNA extracts from 130 known multidrug resistant (MDR) cultured strains and compared with mutations observed in DNA extracts directly from 86 sputum samples from consecutive newly diagnosed cases in Lahore, Pakistan...
2017: PloS One
https://www.readbyqxmd.com/read/28813625/detection-of-first-line-drug-resistance-mutations-and-drug-protein-interaction-dynamics-from-tuberculosis-patients-in-south-india
#14
Somanna Ajjamada Nachappa, Sumana M Neelambike, Chokkanna Amruthavalli, Nallur B Ramachandra
Diagnosis of drug-resistant tuberculosis predominantly relies on culture-based drug susceptibility testing, which take weeks to produce a result and a more time-efficient alternative method is multiplex allele-specific PCR (MAS-PCR). Also, understanding the role of mutations in causing resistance helps better drug designing. AIMS: To evaluate the ability of MAS-PCR in the detection of drug resistance and to understand the mechanism of interaction of drugs with mutant proteins in Mycobacterium tuberculosis...
August 16, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28802406/real-time-pcr-followed-by-high-resolution-melting-curve-analysis-a-rapid-and-pragmatic-approach-for-screening-of-multidrug-resistant-extrapulmonary-tuberculosis
#15
Kusum Sharma, Megha Sharma, Shreya Singh, Manish Modi, Aman Sharma, Pallab Ray, Subhash Varma
INTRODUCTION: Multidrug resistance (MDR) in extrapulmonary tuberculosis (EPTB) is a diagnostic challenge in an endemic country like India. Timely detection of MDR-TB can contribute to a better patient outcome. OBJECTIVE: To perform real-time PCR (qPCR) using rpoB, mpb64 and IS6110 gene on a variety of EPTB samples and to compare the performance of different gene targets. All qPCR positive samples were subjected to high resolution melt-curve analysis (HRM analysis) for rpoB and katG gene to evaluate its potential for MDR screening among different sample types...
September 2017: Tuberculosis
https://www.readbyqxmd.com/read/28780247/direct-detection-of-mycobacterium-tuberculosis-and-drug-resistance-in-respiratory-specimen-using-abbott-realtime-mtb-detection-and-rif-inh-resistance-assay
#16
Kingsley King-Gee Tam, Kenneth Siu-Sing Leung, Sabrina Wai-Chi To, Gilman Kit-Hang Siu, Terrence Chi-Kong Lau, Victor Chi-Man Shek, Cindy Wing-Sze Tse, Samson Sai-Yin Wong, Pak-Leung Ho, Wing-Cheong Yam
Abbott RealTime MTB (Abbott-RT) in conjunction with Abbott RealTime MTB RIF/INH Resistance (Abbott-RIF/INH) is a new, high-throughput automated nucleic acid amplification platform (Abbott-MDR) for detection of Mycobacterium tuberculosis complex (MTBC) and the genotypic markers for rifampicin (RIF) and isoniazid (INH) resistance directly from respiratory specimens. This prospective study evaluated the diagnostic performance of this new platform for MTBC and multidrug-resistant tuberculosis (MDR-TB) using 610 sputum specimens in a tuberculosis high-burden setting...
July 1, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28771786/case-of-mycobacterium-haemophilum-misdiagnosed-as-mycobacterium-intracellulare-due-to-one-base-insertion-in-the-bacterial-genome
#17
Rika Nishikawa, Yozo Yamada, Haruhisa Kanki, Hiroshi Matsuoka, Tatsuya Nakamura, Takumi Jikimoto, Mari Kusuki, Norihisa Ishii, Kenichiro Ohnuma, Kazue Nakanaga, Chikako Nishigori
Mycobacterium haemophilum is a slow-growing, non-tuberculous mycobacteria that causes cutaneous infection. We describe a case of cutaneous infection in a 68-year-old Japanese man with polymyositis. This was caused by M. haemophilum harboring one base insertion in gene sequence. At first, the causal microorganism was misidentified as M. intracellulare by COBAS(®) TaqMan(®) MAI test. However, poor growth on Ogawa media and growth enhancement on 7H11C agar around a hemin-containing disk prompted us to reinvestigate the causal microorganisms, which were revealed to be M...
August 3, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28760681/genetic-diversity-of-mycobacterium-tuberculosis-and-transmission-associated-with-first-line-drug-resistance-a-first-analysis-in-jalisco-mexico
#18
Gladys Lopez-Avalos, Guadalupe Gonzalez-Palomar, Martín Lopez-Rodriguez, Carlos Arturo Vazquez-Chacon, Gustavo Mora-Aguilera, Juan Antonio Gonzalez-Barrios, Juan Carlos Villanueva-Arias, Manuel Sandoval-Diaz, Ulises Miranda-Hernández, Ikuri Alvarez-Maya
OBJECTIVES: To analyze the frequency of gene mutations associated to drug resistant in clinical samples from population of the Jalisco state (Mexico) and then evaluate the genetic variability of TB and MDR-TB strains to describe the frequency of M. tuberculosis families. METHODS: We analyzed the clinical isolates of M. tuberculosis obtained from Jalisco state of Mexico. Isolates were subjected to drug susceptibility testing and mutations were characterized by sequencing, followed by genotyping using spoligotyping and Interspersed Repetitive Units-Variable Number of Tandem Repeat (MIRU-VNTR)...
July 28, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28756826/frequency-of-multi-drug-resistance-and-mutations-in-mycobacterium-tuberculosis-isolates-from-punjab-state-of-india
#19
Ritu Singhal, Jyoti Arora, Grish C Sah, Manpreet Bhalla, Rohit Sarin, Vithal Prasad Myneedu
Data regarding prevalence of multi-drug resistant tuberculosis (MDR-TB) and associated common mutations is scarce from Punjab region. The study was designed to determine rate of MDR-TB among presumptive MDR-TB from Punjab and mutation patterns using GenoType MTBDRplus assay. Total of 812 consecutive sputum samples were received from January 2012 to July 2013, from 14 districts of Punjab at the National Reference Laboratory at New Delhi for diagnosis of MDR-TB as hand holding activity. Presumptive MDR-TB patients were identified on basis of criterion B defined by the programme...
September 2017: Journal of Epidemiology and Global Health
https://www.readbyqxmd.com/read/28749770/in-silico-prediction-of-antibiotic-resistance-in-mycobacterium-ulcerans-agy99-through-whole-genome-sequence-analysis
#20
Sushim Kumar Gupta, Michel Drancourt, Jean-Marc Rolain
Buruli ulcer is an emerging infectious disease caused by Mycobacterium ulcerans that has been reported from 33 countries. Antimicrobial agents either alone or in combination with surgery have been proved to be clinically relevant and therapeutic strategies have been deduced mainly from the empirical experience. The genome sequences of M. ulcerans strain AGY99, M. ulcerans ecovar liflandii, and three Mycobacterium marinum strains were analyzed to predict resistance in these bacteria. Fourteen putative antibiotic resistance genes from different antibiotics classes were predicted in M...
September 2017: American Journal of Tropical Medicine and Hygiene
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