Federica Repele, Tonino Alonzi, Assunta Navarra, Chiara Farroni, Andrea Salmi, Gilda Cuzzi, Giovanni Delogu, Gina Gualano, Vincenzo Puro, Gabriella De Carli, Enrico Girardi, Fabrizio Palmieri, Adrian R Martineau, Delia Goletti
OBJECTIVES: To investigate whether Mycobacterium tuberculosis (Mtb) DNA is detected in peripheral blood mononuclear cells (PBMC) of subjects with tuberculosis (TB) or TB infection (TBI) living in a low TB burden country. METHODS: We prospectively enrolled 57 TB-patients, 41 TBI-subjects and 39 controls in Rome, Italy. PBMC were isolated, CD34+ and CD34- cells were immunomagnetic separated, DNA was extracted and digital PCR for IS6110 and rpoB sequences was used to detect Mtb DNA in PBMC subsets and unfractionated PBMC...
March 6, 2024: International Journal of Infectious Diseases: IJID