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https://www.readbyqxmd.com/read/28211316/acinetobacter-defluvii-sp-nov-recovered-from-hospital-sewage
#1
Yiyi Hu, Yu Feng, Xiaoxia Zhang, Zhiyong Zong
A Gram-negative, non-motile Acinetobacter strain, WCHA30, was isolated from hospital sewage in West China Hospital of Sichuan University in Chengdu, southwestern China. Strain WCHA30 is a non-spore-forming, catalase-positive, oxidase-negative, strictly aerobic coccobacillus. The DNA G+C content was 38 mol%. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that the strain was distinct from any previously described species of the genus Acinetobacter. Strain WCHA30 could be distinguished from all known Acinetobacter species by its ability to assimilate β-alanine but not L-glutamate...
February 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28207789/carbapenem-resistance-and-pathogenicity-of-bovine-acinetobacter-indicus-like-isolates
#2
Peter Klotz, Stephan Göttig, Ursula Leidner, Torsten Semmler, Sandra Scheufen, Christa Ewers
The objective of this study was to characterize blaOXA-23 harbouring Acinetobacter indicus-like strains from cattle including genomic and phylogenetic analyses, antimicrobial susceptibility testing and evaluation of pathogenicity in vitro and in vivo. Nasal and rectal swabs (n = 45) from cattle in Germany were screened for carbapenem-non-susceptible Acinetobacter spp. Thereby, two carbapenem resistant Acinetobacter spp. from the nasal cavities of two calves could be isolated. MALDI-TOF mass spectrometry and 16S rDNA sequencing identified these isolates as A...
2017: PloS One
https://www.readbyqxmd.com/read/28197340/phenotypic-and-genotypic-analysis-of-multidrug-resistant-mycobacterium-tuberculosis-isolates-from-sudanese-patients
#3
Solima M A Sabeel, Mohamed Ahmed Salih, Manasik Ali, Salah-Eldin El-Zaki, Nadir Abuzeid, Zeinab Abubaker Mohammed Elgadi, Hisham N Altayb, Asrar M A Elegail, Nuha Y Ibrahim, Bahaeldin K Elamin
Background. Currently, mutations in rpoB, KatG, and rrs genes and inhA promoter were considered to be involved in conferring resistance to rifampicin, isoniazid, and streptomycin in Mycobacterium tuberculosis (MTB). Objective. The aims of this study were to detect the prevalence of first-line tuberculosis (TB) drug resistance among a group of previously treated and newly detected TB patients, to determine the association between prevalence of multidrug resistance (MDR) and demographic information (age and sex), to explain genes correlated with MDR Mycobacterium tuberculosis, and to characterize MTB via 16S ribosomal RNA (16S rRNA) analysis...
2017: Tuberculosis Research and Treatment
https://www.readbyqxmd.com/read/28193207/a-novel-point-mutation-in-rpob-improves-osmotolerance-and-succinic-acid-production-in-escherichia-coli
#4
Mengyong Xiao, Xinna Zhu, Hongtao Xu, Jinlei Tang, Ru Liu, Changhao Bi, Feiyu Fan, Xueli Zhang
BACKGROUND: Escherichia coli suffer from osmotic stress during succinic acid (SA) production, which reduces the performance of this microbial factory. RESULTS: Here, we report that a point mutation leading to a single amino acid change (D654Y) within the β-subunit of DNA-dependent RNA polymerase (RpoB) significantly improved the osmotolerance of E. coli. Importation of the D654Y mutation of RpoB into the parental strain, Suc-T110, increased cell growth and SA production by more than 40% compared to that of the control under high glucose osmolality...
February 13, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28180951/bradyrhizobium-centrolobii-and-bradyrhizobium-macuxiense-sp-nov-isolated-from-centrolobium-paraense-grown-in-soil-of-amazonia-brazil
#5
Daniele C Michel, Samuel R Passos, Jean L Simões-Araujo, Alexandre C Baraúna, Krisle da Silva, Marcia M Parma, Itamar S Melo, Sofie E De Meyer, Graham O'Hara, Jerri E Zilli
Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36 °C (optimal 28 °C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity...
February 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28167522/decreased-expression-of-stable-rna-can-alleviate-the-lethality-associated-with-rnase-e-deficiency-in-escherichia-coli
#6
P Himabindu, K Anupama
The endoribonuclease RNase E participates in mRNA degradation, rRNA processing and tRNA maturation in Escherichia coli, but the precise reasons for its essentiality are unclear and much debated. The enzyme is most active on RNA substrates with a 5' -terminal monophosphate, which is sensed by a domain in the enzyme that includes residue R169; E. coli also possesses a 5' -pyrophosphohydrolase RppH that catalyses conversion of 5' -terminal-triphosphate to -monophosphate on RNAs. Although the C-terminal half (CTH), beyond residue approximately 500, of RNase E is dispensable for viability, the ΔCTH mutation is lethal when combined with an R169Q mutation or with deletion of rppH In this work, we show that both these lethalities can be rescued in derivatives in which four, or five, of the seven rrn operons in the genome have been deleted...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28165925/molecular-detection-of-candidatus-bartonella-mayotimonensis-in-north-american-bats
#7
Thomas M Lilley, Cali A Wilson, Riley F Bernard, Emma V Willcox, Eero J Vesterinen, Quinn M R Webber, Laura Kurpiers, Jenni M Prokkola, Imran Ejotre, Allen Kurta, Kenneth A Field, DeeAnn M Reeder, Arto T Pulliainen
Candidatus Bartonella mayotimonensis was detected in 2010 from an aortic valve sample of a patient with endocarditis from Iowa, the United States of America. The environmental source of the potentially new endocarditis-causing Bartonella remained elusive. We set out to study the prevalence and diversity of bat-associated Bartonella in North America. During 2015, mist nets and harp traps were used to capture 92 bats belonging to two species: little brown myotis (Myotis lucifugus Le Conte 1831, n = 73) and the gray myotis (M...
February 6, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28163698/bacillus-amyloliquefaciens-bacillus-velezensis-and-bacillus-siamensis-form-an-operational-group-b-amyloliquefaciens-within-the-b-subtilis-species-complex
#8
Ben Fan, Jochen Blom, Hans-Peter Klenk, Rainer Borriss
The plant growth promoting model bacterium FZB42(T) was proposed as the type strain of Bacillus amyloliquefaciens subsp. plantarum (Borriss et al., 2011), but has been recently recognized as being synonymous to Bacillus velezensis due to phylogenomic analysis (Dunlap C. et al., 2016). However, until now, majority of publications consider plant-associated close relatives of FZB42 still as "B. amyloliquefaciens." Here, we reinvestigated the taxonomic status of FZB42 and related strains in its context to the free-living soil bacterium DSM7(T), the type strain of B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28137804/a-clostridium-difficile-lineage-endemic-to-costa-rican-hospitals-is-multidrug-resistant-by-acquisition-of-chromosomal-mutations-and-novel-mobile-genetic-elements
#9
Gabriel Ramírez-Vargas, Carlos Quesada-Gómez, Luis Acuña-Amador, Diana López-Ureña, Tatiana Murillo, María Del Mar Gamboa-Coronado, Esteban Chaves-Olarte, Nicholas Thomson, Evelyn Rodríguez-Cavallini, César Rodríguez
The antimicrobial resistance rates and levels recorded for Clostridium difficile are on the rise. This study reports the nature, levels, diversity, and genomic context of the antimicrobial resistance of human C. difficile isolates from the NAPCR1/RT012/ST54 genotype, which caused an outbreak in 2009 and is endemic in Costa Rican hospitals. To this end, we determined the susceptibility of 38 NAPCR1 isolates to ten antibiotics from seven classes using E-tests or macrodilution tests and examined 31 NAPCR1 whole-genome sequences to identify single nucleotide polymorphisms and genes that could explain the observed resistance phenotypes...
January 30, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28128318/pantoea-alhagi-a-novel-endophytic-bacterium-with-ability-to-improve-growth-and-drought-tolerance-in-wheat
#10
Chaoqiong Chen, Kaiyun Xin, Hao Liu, Juanli Cheng, Xihui Shen, Yao Wang, Lei Zhang
A novel strain LTYR-11Z(T) that exhibited multiple plant growth promoting (PGP) traits was isolated from the surface-sterilized leaves of Alhagi sparsifolia Shap. (Leguminosae), which reprsents one of the top drought tolerant plants in north-west China. Phylogenetic analysis of 16S rRNA gene sequences and multilocus sequence analysis based on partial sequences of atpD, gyrB, infB and rpoB genes revealed that strain LTYR-11Z(T) was a member of the genus Pantoea, with Pantoea theicola NBRC 110557(T) and Pantoea intestinalis DSM 28113(T) as the closest phylogenetic relatives...
January 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28126698/identification-of-arcanobacterium-hippocoleae-by-maldi-tof-ms-analysis-and-by-various-genotypical-properties
#11
J-P Wickhorst, O Sammra, A A Hassan, M Alssashen, C Lämmler, E Prenger-Berninghoff, M Erhard, M Metzner, K Paschertz, M Timke, A Abdulmawjood
In the present study an Arcanobacterium hippocoleae strain isolated from a uterus swab of an apparently healthy mare could be identified by phenotypic properties, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region and the genes encoding the β subunit of bacterial RNA polymerase (rpoB), elongation factor tu (tuf) and glyceraldehyde 3-phosphate dehydrogenase (gap). The presented data are one of the few reports about the species A...
January 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28126040/campylobacter-ornithocola-sp-nov-a-new-member-of-the-campylobacter-lari-group-isolated-from-wild-bird-faecal-samples
#12
Alberto Caceres, Ivo Muñoz, Gregorio Iraola, Florencia Díaz-Viraqué, Luis Collado
During a study on the prevalence and diversity of campylobacteria in wild birds faecal samples in the city of Valdivia (South of Chile) 17 Gram-negative, curved rod isolates, were initially identified as Campylobacter lari by PCR-RFLP. Further identification by 16S rRNA sequence analysis revealed that they formed a distinct group in the genus Campylobacter. This unique position was confirmed by rpoB, atpA and cpn60 gene sequence analysis. The average nucleotide identity between the representative strain WBE38T and the type strain of the closest taxon Campylobacter lari subsp...
January 23, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28122871/multicenter-evaluation-of-the-portrait-staph-id-r-blood-culture-panel-for-the-rapid-identification-of-staphylococci-and-detection-of-meca-gene
#13
Gerald A Denys, Vanessa Collazo-Velez, Stephen Young, Judy A Daly, Marc Roger Couturier, Matthew L Faron, Blake W Buchan, Nathan Ledeboer
Blood stream infections are a leading cause of morbidity and mortality in the United States and are associated with increased healthcare cost. We evaluated the Portrait Staph ID/R Blood Culture Panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake City, UT) for the rapid and simultaneous identification (ID) of Staphylococcus aureus, S. lugdunensis and Staphylococcus species to the genus level and the detection of mecA gene directly from a positive blood culture bottle. A total of 765 BACTEC® bottles demonstrating Gram positive cocci in singles or clusters were tested during the prospective trial at 3 clinical sites...
January 25, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28120200/kribbella-soli-sp-nov-isolated-from-soil
#14
Fadime Ozdemir-Kocak, Hayrettin Saygin, Salih Saricaoglu, Demet Cetin, Kiymet Guven, Cathrin Spröer, Peter Schumann, Hans-Peter Klenk, Nevzat Sahin, Kamil Isik
A novel actinobacterial strain, designated FMN22(T), was isolated from soil and characterised using a polyphasic approach. Strain FMN22(T) showed high 16S rRNA gene sequence similarity to Kribbella karoonensis Q41(T) (99.3%), Kribbella shirazensis UTMC 693(T) (99.0%), Kribbella aluminosa HKI 0478(T) (98.9%) and Kribbella hippodromi S1.4(T) (98.6%). Phylogenetic analysis using the 16S rRNA and concatenated gene (gyrB, rpoB, relA, recA and atpD) sequences showed that strain FMN22(T) is closely related to the type strains of K...
January 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28113092/occurrence-of-arcobacter-spp-and-correlation-with-the-bacterial-indicator-of-faecal-contamination-escherichia-coli-in-bivalve-molluscs-from-the-central-adriatic-italy
#15
Francesca Leoni, Serena Chierichetti, Sabrina Santarelli, Giulia Talevi, Laura Masini, Chiara Bartolini, Elena Rocchegiani, M Naceur Haouet, Donatella Ottaviani
A total of 162 samples of bivalve molluscs (45 mussels and 117 clams) collected between December 2012 and 2014 from harvesting areas of the Central Adriatic were analysed by a culturing method for the presence of Arcobacter spp. Species identification was performed by PCR and sequencing analysis of a fragment of the rpoB gene. Overall, Arcobacter species were detected in 30% of samples, specifically 33% clams and 22% mussels. A. butzleri was the most common species (20% of the samples), followed by A. cryaerophilus (9%) and A...
January 17, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28109205/haloterrigena-mahii-sp-nov-an-extreme-halophilic-archaeon-from-the-solar-saltern
#16
Jiun-Yan Ding, Sheng-Chung Chen, Mei-Chin Lai, Tsai-Ling Liao
A pleomorphic, gas vesicle containing, halophilic archaeon, designated strain H13T, was isolated from the solar saltern of the Western Salt Co., Chula Vista, California, USA. Cells of strain H13T were non-motile, rod-shaped and 3-10 μm in length. The optimum growth conditions were 3.5-5.0 M NaCl, 45-55 oC, and pH range of 6.5-8.2. The major polar lipids were C20C20 and C20C25 derivatives of phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and disulfated diglycosyl diether-1 (S2-DGD-1)...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28100602/find-tuberculosis-strain-bank-a-resource-for-researchers-and-developers-working-on-tests-to-detect-mycobacterium-tuberculosis-and-related-drug-resistance
#17
Belay Tessema, Pamela Nabeta, Eloise Valli, Audrey Albertini, Jimena Collantes, Nguyen Huu Lan, Elena Romancenco, Nestani Tukavdze, Claudia M Denkinger, David L Dolinger
BACKGROUND: The spread of multidrug-resistant tuberculosis and extensively drug-resistant TB hampers the global efforts in the fight against tuberculosis. To enhance the development and evaluation of diagnostic tests quickly and efficiently, well characterized strains and samples from drug resistant tuberculosis patients are necessary. In this project, FIND has focused on the collection, characterization and storage of such well-characterized reference materials and to make them available to researchers and developers...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28095503/detection-of-a-potential-new-bartonella-species-candidatus-bartonella-rondoniensis-in-human-biting-kissing-bugs-reduviidae-triatominae
#18
Maureen Laroche, Jean-Michel Berenger, Oleg Mediannikov, Didier Raoult, Philippe Parola
BACKGROUND: Among the Reduviidae family, triatomines are giant blood-sucking bugs. They are well known in Central and South America where they transmit Trypanosoma cruzi to mammals, including humans, through their feces. This parasitic protozoan is the causative agent of Chagas disease, a major public health issue in endemic areas. Because of the medical and economic impact of Chagas disease, the presence of other arthropod-borne pathogens in triatomines was rarely investigated. METHODOLOGY/PRINCIPAL FINDINGS: In this study, seven triatomines species involved in the transmission of T...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28081169/assessment-of-a-96-well-plate-assay-of-quantitative-drug-susceptibility-testing-for-mycobacterium-tuberculosis-complex-in-china
#19
Hui Xia, Yang Zheng, Bing Zhao, Susan van den Hof, Frank Cobelens, YanLin Zhao
OBJECTIVE: To evaluate the performance of the Sensitire MYCOTB MIC Plate (MYCOTB) which could measure the twelve anti-tuberculosis drugs susceptibility on one 96-wells plate. METHODS: A total of 140 MDR-TB strains and 60 non-MDR strains were sub-cultured and 193 strains were finally tested for drug resistance using MYCOTB and agar proportion method (APM) and another 7 strains failed of subculture. The drugs included ofloxacin (Ofx), moxifloxacin (Mfx), rifampin (RFP), amikacin (Am), rifabutin (Rfb), para-aminosalicylic acid (PAS), ethionamide (Eth), isoniazid (INH), kanamycin (Km), ethambutol (EMB), streptomycin (Sm), and cycloserine(Cs)...
2017: PloS One
https://www.readbyqxmd.com/read/28076350/emergence-of-rare-species-of-nontuberculous-mycobacteria-as-potential-pathogens-in-saudi-arabian-clinical-setting
#20
Bright Varghese, Mushira Enani, Mohammed Shoukri, Sahar AlThawadi, Sameera AlJohani, Sahal Al-Hajoj
BACKGROUND: Clinical relevance of nontuberculous mycobacteria (NTM) is increasing worldwide including in Saudi Arabia. A high species diversity of NTM's has been noticed in a recent study. However, the identification in diagnostic laboratories is mostly limited to common species. The impact of NTM species diversity on clinical outcome is so far neglected in most of the clinical settings. METHODOLOGY/PRINCIPAL FINDINGS: During April 2014 to September 2015, a nationwide collection of suspected NTM clinical isolates with clinical and demographical data were carried out...
January 2017: PLoS Neglected Tropical Diseases
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