Read by QxMD icon Read


S Safaei, M Fatahi-Bafghi, Omid Pouresmaeil
Tsukamurella is an aerobic, Gram-positive and nonmotile bacterium. It was first isolated in 1941 from the mycetoma and ovaries of the bedbug. The primary strains were named Corynebacterium paurometabolum and Gordona aurantiaca and are different from the Collins et al. , 1988 classification of the new Tsukamurella genus. Human infections with Tsukamurella species are rare because the species is a kind of saprophyte bacterium; however, most information regarding this species comes from case reports. Molecular markers for the identification Tsukamurella include sequencing of 16S rRNA, groEL, rpoB, secA1 and ssrA genes...
March 2018: New Microbes and New Infections
Amanda Bonalume Cordeiro de Morais, Carmen Alicia Daza Bolaños, Ana Carolina Alves, Cássia Yumi Ikuta, Gustavo Henrique Batista Lara, Marcos Bryan Heinemann, Rogério Giuffrida, Fernando Paganini Listoni, Mateus de Souza Ribeiro Mioni, Rodrigo Garcia Motta, Shinji Takai, Márcio Garcia Ribeiro
Mycobacterium species and the virulence-associated proteins (vapA, vapB, and vapN genes) of Rhodococcus equi isolated from 330 lymph nodes of collared peccaries (Tayassu tajacu) and white-lipped peccaries (Tayassu pecari) intended for human consumption were investigated. Thirty-six (10.9%) R. equi strains were isolated; 3.3% (n = 11/330) were from white-lipped peccary lymph nodes, and 7.6% (25/330) were from collared peccary lymph nodes. Among the 11 isolates of R. equi from the white-lipped peccaries, 90...
March 15, 2018: Tropical Animal Health and Production
Nomakorinte Gcebe, Victor P M G Rutten, Nicolaas Gey van Pittius, Brendon Naicker, Anita L Michel
Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross-reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa...
March 15, 2018: International Journal of Systematic and Evolutionary Microbiology
Paolo Miotto, Andrea M Cabibbe, Emanuele Borroni, Massimo Degano, Daniela M Cirillo
Low-level rifampicin resistance associated with specific rpoB mutations (referred as "disputed") in Mycobacterium tuberculosis is easily missed by some phenotypic methods. To understand the mechanism by which some mutations are systematically missed by MGIT phenotypic testing we performed an in silico analysis of their effect on the structural interaction between the RpoB protein and rifampicin. We also characterized 24 representative clinical isolates by determining minimum inhibitory concentrations (MICs) on 7H10 agar, and testing them by an extended MGIT protocol...
March 14, 2018: Journal of Clinical Microbiology
Satyendra Kumar Singh, Ajit Kumar, Gopal Nath, Tej Bali Singh, Mukti Nath Mishra
BACKGROUND: WHO MDT is the main drug regimen for treating leprosy and has been used for more than three decades. Many cases of relapse of leprosy have been reported, which points towards the emergence of drug resistance with the antileprotic drugs. OBJECTIVES: To find the resistance with the antileprotic drugs by detecting the mutations in drug resistance determining region of the rpoB, folP1 and gyrA genes of Mycobacterium leprae. METHODS: Leprosy patients with bacterial index ≥2 were included in the study...
March 14, 2018: Indian Journal of Dermatology, Venereology and Leprology
Luz I Valenzuela-García, Víctor M Ayala-García, Ana G Regalado-García, Peter Setlow, Mario Pedraza-Reyes
The absence of base excision repair (BER) proteins involved in processing ROS-promoted genetic insults activates a DNA damage scanning (DisA)-dependent checkpoint event in outgrowing Bacillus subtilis spores. Here, we report that genetic disabling of transcription-coupled repair (TCR) or nucleotide excision repair (NER) pathways severely affected outgrowth of ΔdisA spores, and much more so than the effects of these mutations on log phase growth. This defect delayed the first division of spore's nucleoid suggesting that unrepaired lesions affected transcription and/or replication during outgrowth...
March 13, 2018: MicrobiologyOpen
Viktorija Kirillova, Petras Prakas, Rafael Calero-Bernal, Inese Gavarāne, José Luis Fernández-García, Manuel Martínez-González, Eglė Rudaitytė-Lukošienė, Miguel Ángel Habela Martínez-Estéllez, Dalius Butkauskas, Muza Kirjušina
BACKGROUND: Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different populations in Latvia, Lithuania and Spain. METHODS: Muscle samples from 411 red foxes (Vulpes vulpes) and 269 racoon dogs (Nyctereutes procyonoides) from Latvia, 41 red foxes from Lithuania and 22 red foxes from Spain were examined for the presence of Sarcocystis sarcocysts by light microscopy (LM)...
March 12, 2018: Parasites & Vectors
Yeo Jun Yun, Jong Seok Lee, Je Chul Yoo, Eunjin Cho, Dahee Park, Yoon Hoh Kook, Keun Hwa Lee
BACKGROUND: Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea...
March 7, 2018: Tuberculosis and Respiratory Diseases
Antonio Alfonzo, Raimondo Gaglio, Alessandro Miceli, Nicola Francesca, Rosalia Di Gerlando, Giancarlo Moschetti, Luca Settanni
Microbiological, chemical and physical parameters of minimally processed red chicory (Cichorium intybus L.) subjected to two different transformation processes were investigated. A classic ready-to-eat (RTE) process (P1) and a production without cutting (P2) were monitored during refrigerated (4 °C) storage (15 d). Total mesophilic microorganisms, total psychrotrophic microorganisms and pseudomonads were detected at the highest cell densities in all samples. Presumptive Pseudomonas population dominated the cultivable microbial community of RTE red chicory and were characterized genetically...
August 2018: Food Microbiology
P R F Adkins, S Dufour, J N Spain, M J Calcutt, T J Reilly, G C Stewart, J R Middleton
The purpose of this study was to investigate non-aureus Staphylococcus spp. intramammary infections (IMI) in periparturient heifers and determine the relationship of precalving body site isolation with precalving IMI and postcalving IMI using molecular speciation and strain-typing methods. Primiparous heifers were enrolled at approximately 14 d before expected calving date. Precalving mammary quarter secretions and body site swabbing samples (teat skin, inguinal skin, muzzle, and perineum) were collected. Postcalving, mammary quarter milk samples were collected for culture and somatic cell counting...
March 7, 2018: Journal of Dairy Science
Vladimír Kmeť, Anna Čuvalová, Michal Stanko
A total of 39 coagulase-negative staphylococci and seven Staphylococcus aureus strains were isolated from small mammal feces, i.e., the striped field mouse (Apodemus agrarius) and the yellow-necked mouse (A. flavicollis) in two sampling areas, deciduous forest and karst plains. MALDI-TOF analysis revealed five species of coagulase-negative staphylococci: S. sciuri, S. hominis, S. warneri, S. haemolyticus, and S. xylosus. All strains were susceptible to tetracycline, linezolid, vancomycin, and teicoplanin. Three MRSA strains with the mecA gene were detected...
March 9, 2018: Folia Microbiologica
David A Jaffe, Bruno B Chomel, Rickie W Kasten, Edward B Breitschwerdt, Ricardo G Maggi, Ashleigh McLeish, Ulrike Zieger
Many mammals are established hosts for the vector borne bacterial genus, Bartonella. Small Indian mongooses (Herpestes auropunctatus) have only been reported as a possible host for Bartonella henselae in southern Japan. Confirming Bartonella presence in mongooses from other regions in the world may support their role as potential reservoirs of this human pathogen. Specifically, documenting Bartonella in Caribbean mongooses would identify a potential source of zoonotic risk with mongoose-human contact in the New World...
March 2018: Veterinary Microbiology
Karthic Rajamanickam, Sidney Hayes
The temperate bacteriophage lambda (λ) CII protein is a positive regulator of transcription from promoter pE , a component of the lysogenic response. The expression of cII was examined in vectors devoid of phage transcription-modulating elements. Their removal enabled evaluating if the expression of the small RNA OOP, on its own, could suppress CII activities, including complementing for a lysogenic response, cell toxicity and causing rapid cellular loss of ColE1 plasmids. The results confirm that OOP RNA expression from the genetic element pO-oop-to can prevent the ability of plasmid-encoded CII to complement for a lysogenic response, suggesting that it serves as a powerful regulatory pivot in λ development...
March 7, 2018: Viruses
T Y Zuo, Q Y Liu, M Y Gan, Q Gao
Objective: To comprehensively identify compensatory mutations in rpoA, rpoB and rpoC genes of rifampicin-resistant Mycobacterium tuberculosis (RIF-r MTB) and to evaluate the effect of rifampicin-resistant mutation type and lineage background on occurrence of compensatory mutations. Methods: Published MTB whole genome sequencing data were searched and downloaded. RIF-r MTB was identified through known rifampicin-resistant mutations. Based on parallel evolutionary patterns, we identified putative compensatory mutations in the phylogenetic tree and calculated proportions of accumulating compensatory mutations in each rifampicin-resistant mutations' type and lineage background of RIF-r MTB...
March 12, 2018: Chinese Journal of Tuberculosis and Respiratory Diseases
Hang Dai, Yonglu Wang, Yujie Fang, Zhenzhou Huang, Biao Kan, Duochun Wang
Two strains of Gram-stain-negative, facultatively anaerobic short-rod bacteria were recovered from two different food samples in Ma'anshan city, Anhui province, China in 2008. The bacteria were characterized in a polyphasic taxonomic study that included phenotypic, phylogenetic and genotypic methodologies. Phylogenetic analysis of the 16S rRNA gene demonstrated that the two strains belonged to the genus Proteus and were most similar to Proteus vulgaris ATCC 29905T with a score of 99.7 %. Phylogenetic analysis of the rpoB gene placed the two strains into a cluster with a distinctly interspecies phylogenetic branch that was clearly separated from six type strains of the genus Proteus, with the most closely related species being Proteus mirabilis ATCC 29906T ...
March 6, 2018: International Journal of Systematic and Evolutionary Microbiology
Dmitriy V Volokhov, Megan Amselle, Sonya Bodeis-Jones, Pierluigi Delmonte, Shuping Zhang, Maureen K Davidson, Frances M Gulland, Vladimir E Chizhikov
Three independent strains of Neisseria sp. were isolated from the oral cavity of California sea lions (Zalophus californianus) that were admitted to The Marine Mammal Center facilities in California, USA. The strains were isolated from oral swabs by cultivation on Trypticase Soy agar with 5% sheep blood under aerobic conditions. The 16S rRNA gene sequence of these three strains shared 99% similarity, but demonstrated only 97-98% nucleotide similarity to the phylogenetically closest relatives such as N. canis, N...
March 5, 2018: Archives of Microbiology
Akira Yokoyama, Hidenori Kage, Yuki Ohama, Tomoaki Sato, Naoya Miyashita, Satoshi Noguchi, Shu Okugawa, Yasuhiro Yamauchi, Kyoji Moriya, Takahide Nagase
Identification of microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely accepted. However, the significance of MALDI-TOF MS for identifying mycobacteria, particularly rare nontuberculous mycobacteria, has not been established. M. heckeshornense is one such bacteria, and distinguishing it from M. xenopi is difficult. The patient was a 40-year-old man with Behҫet's disease who had started treatment with prednisolone and azathioprine. A lung nodule in the right lower lobe was pointed out, and it increased in size 6 months later...
March 1, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Wen-Ming Chen, Pei-Bei Xie, Ming-Yuan Hsu, Shih-Yi Sheu
A bacterial strain designated KMB9T was isolated from a freshwater pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain KMB9T were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by means of a monopolar flagellum, non-spore-forming and rods surrounded by a thick capsule and forming white-coloured colonies. Growth occurred at 20-40 °C (optimum, 25-37 °C), at pH 6.5-7.5 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene and four housekeeping gene sequences (recA, rpoA, rpoB and atpD) showed that strain KMB9T forms a distinct phyletic line within the family Alcaligenaceae, and the levels of 16S rRNA gene sequence similarity to its closest relatives with validly published names were less than 93...
March 2, 2018: International Journal of Systematic and Evolutionary Microbiology
Vera Safronova, Andrey Belimov, Anna Sazanova, Elizaveta Chirak, Alla Verkhozina, Irina Kuznetsova, Evgeny Andronov, Jan Puhalsky, Igor Tikhonovich
Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification of the isolates sequencing of rrs, ITS (internal transcribed spacer) region and housekeeping genes recA, glnII and rpoB was used. Nine fast-growing isolates were Mesorhizobium-related: eight strains were identified as M. japonicum and one isolate belonged to M. kowhaii. The only slow-growing isolate was identified as Bradyrhizobium sp. Two strains, M. japonicum Opo-242 and Bradyrhizobium sp...
March 2, 2018: Molecular Plant-microbe Interactions: MPMI
Emmanuelle Cambau, Paul Saunderson, Masanori Matsuoka, Stewart T Cole, Masanori Kai, Philip Suffys, Patricia S Rosa, Diana Williams, Umesh D Gupta, Mallika Lavania, Nora Cordona, Yuji Miyamoto, Deanna Hagge, Aparna Srikantam, Wang Hongseng, Agsuni Indropo, Varalakshmi Vissa, Roch C Johnson, Bertrand Cauchoix, Vijaykumar K Pannikar, Erwin Awd Cooreman, Vr Rao Pemmaraju, Laura Gillini
OBJECTIVES: antimicrobial resistance (AMR) is a priority for surveillance in bacterial infections. For leprosy, AMR could not be assessed so far since Mycobacterium leprae does not grow in vitro. We aim to obtain AMR data using molecular detection of resistance genes and to conduct a prospective open survey of resistance to antileprosy drugs in endemic countries through a WHO surveillance network. METHODS: From 2009 to 2015, multi-bacillary leprosy cases at sentinel sites of 19 countries, were studied for resistance to rifampicin, dapsone and ofloxacin by PCR sequencing of the drug resistance determining regions (DRDR) of the genes rpoB, folP1 and gyrA...
February 26, 2018: Clinical Microbiology and Infection
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"