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https://www.readbyqxmd.com/read/27915419/necrotizing-meningoencephalitis-caused-by-sarcocystis-falcatula-in-bare-faced-ibis-phimosus-infuscatus
#1
Guilherme Konradt, Matheus Viezzer Bianchi, Ronaldo Viana Leite-Filho, Bruna Zafalon da Silva, Rodrigo Martins Soares, Saulo Petinatti Pavarini, David Driemeier
The infection by S. falcatula is commonly associated with respiratory disease in captive psittacine birds, with a few case reports of this protozoan causing encephalitis in wild birds. We describe the clinical, pathological, and molecular aspects of an infection by S. falcatula in a bare-faced ibis (Phimosus infuscatus). Clinically, wing paralysis and mild motor incoordination were observed. At necropsy, the telencephalic cortex showed multifocal to coalescing yellowish soft areas. Histologically, multifocal to coalescent nonsuppurative necrotizing meningoencephalitis of telencephalic cortex, cerebellum, and brainstem was observed...
December 3, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27908688/increased-production-of-l-serine-in-escherichia-coli-through-adaptive-laboratory-evolution
#2
Hemanshu Mundhada, Jose S Miguel, Konstantin Schneider, Anna Koza, Hanne B Christensen, Tobias Klein, Patrick V Phaneuf, Markus Herrgard, Adam M Feist, Alex T Nielsen
L-serine is a promising building block biochemical with a high theoretical production yield from glucose. Toxicity of L-serine is however prohibitive for high-titer production in E. coli. Here, E. coli lacking L-serine degradation pathways was evolved for improved tolerance by gradually increasing L-serine concentration from 3 to 100g/L using adaptive laboratory evolution (ALE). Genome sequencing of isolated clones revealed multiplication of genetic regions, as well as mutations in thrA, thereby showing a potential mechanism of serine inhibition...
November 28, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27903459/true-rifampicin-resistance-missed-by-the-mgit-prevalence-of-this-pheno-genotype-in-the-uk-and-ireland-after-18-month-surveillance
#3
Ximena Gonzalo, Pauline Claxton, Tim Brown, Lorraine Montgomery, Margaret Fitzgibbon, Ian Laurenson, Francis Drobniewski
OBJECTIVES: To characterise rifampicin resistant strains missed by the MGIT 960 system but not by egg-based media in the UK and Ireland and to ascertain their prevalence. METHODS: All strains sent for second line susceptibility testing were prospectively collected. Drug Susceptibility Testing was performed by Resistance Ratio (RR), Proportion Method (PM), MGIT 960 and MIC determination by microdilution. Rifampicin resistance conferring mutations were detected with line probe assays and sequencing...
November 26, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27902737/a-comparison-of-the-sensititre-mycotb-plate-the-bactec-mgit-960-and-a-microarray-based-molecular-assay-for-the-detection-of-drug-resistance-in-clinical-mycobacterium-tuberculosis-isolates-in-moscow-russia
#4
Elena Y Nosova, Danila V Zimenkov, Anastasia A Khakhalina, Alexandra I Isakova, Ludmila Y Krylova, Marina V Makarova, Ksenia Y Galkina, Maria A Krasnova, Svetlana G Safonova, Vitaly I Litvinov, Dmitry A Gryadunov, Elena M Bogorodskaya
BACKGROUND: The goal of this study was to compare the consistency of three assays for the determination of the drug resistance of Mycobacterium tuberculosis (MTB) strains with various resistance profiles isolated from the Moscow region. METHODS: A total of 144 MTB clinical isolates with a strong bias toward drug resistance were examined using Bactec MGIT 960, Sensititre MycoTB, and a microarray-based molecular assay TB-TEST to detect substitutions in the rpoB, katG, inhA, ahpC, gyrA, gyrB, rrs, eis, and embB genes that are associated with resistance to rifampin, isoniazid, fluoroquinolones, second-line injectable drugs and ethambutol...
2016: PloS One
https://www.readbyqxmd.com/read/27902437/development-validation-and-implementation-of-a-quadruplex-real-time-pcr-assay-for-identification-of-potentially-toxigenic-corynebacteria
#5
Aruni De Zoysa, Androulla Efstratiou, Ginder Mann, Timothy G Harrison, Norman K Fry
Toxigenic corynebacteria are uncommon in the UK, however laboratory confirmation by the national reference laboratory (NRL) can inform public health action according to national guidelines. Standard phenotypic tests for identification and toxin expression of isolates can take from ≥24 h to ≥48 h, from receipt. To decrease the time to result, a real-time PCR (qPCR) assay was developed for confirmation of both identification of Corynebacterium diphtheriae and Corynebacterium ulcerans/ Corynebacterium pseudotuberculosis and detection of the diphtheria toxin gene...
November 1, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902304/pseudomonas-canadensis-sp-nov-a-biological-control-agent-isolated-from-a-field-plot-under-long-term-mineral-fertilization
#6
James T Tambong, Renin Xu, Eden Bromfield
The bacterial strain 2-92T isolated from a field plot under long-term (>40 years) mineral fertilization exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T is gram-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. Optimal growth temperature of strain 2-92T is 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas...
November 28, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902286/macrococcus-canis-sp-nov-a-skin-bacterium-associated-with-infections-in-dogs
#7
Stefanie Gobeli Brawand, Kerstin Cotting, Elena Gómez-Sanz, Alexandra Collaud, Andreas Thomann, Isabelle Brodard, Sabrina Rodriguez Campos, Christian Strauss, Vincent Perreten
Gram-stain positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA was Macrococcus caseolyticus with 99.7% sequence identity, but compared to M. caseolyticus, the novel strains shared only 90.8% to 93.5% DNA identity to cpn60, dnaJ, rpoB, and sodA partial genes, respectively. The two species also exhibited other phenotypic characteristics and the majority displayed a visible haemolysis on sheep blood agar, while M...
November 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902257/acinetobacter-larvae-sp-nov-isolated-from-the-larval-gut-of-omphisa-fuscidentalis
#8
Song Liu, Yanwei Wang, Zhiyong Ruan, Kedong Ma, Bo Wu, Yansheng Xu, Jingli Wang, Yang You, Mingxiong He, Guoquan Hu
A Gram-stain-negative, non-spore-forming, non-motile, and aerobic coccobacilli shaped strain, designated BRTC-1T, was isolated from the gut of Omphisa fuscidentalis Hampson, which is a larva of a moth and was collected from Xishuangbanna Dai Autonomous Prefecture in China. The isolate was found to grow at NaCl concentrations of 0-5 % (w/v) (optimum: 0 %), 10-45 ℃ (optimum: 30-35 ℃) and pH 5.0-9.0 (optimum: 6.0) on TSA. Analysis of the 16S rRNA gene sequence indicated that the isolate belongs to the genus Acinetobacter and most closely related to Acinetobacter rudis LMG 26107T, Acinetobacter guillouiae LMG 988T and Acinetobacter bereziniae LMG 1003T with 96...
November 8, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902229/citrobacter-europaeus-sp-nov-a-novel-citrobacter-species-isolated-from-water-and-human-faecal-samples
#9
Sylvain Brisse, Teresa G Ribeiro, Dominique Clermont, Raquel Branquinho, Elisabete Maria Pereira Machado, Luisa Peixe
Strains 97/79T and A121, recovered respectively from human faeces and well waters, were compared to currently known Citrobacter species using genotypic and phenotypic approaches. Multilocus sequence analysis based on housekeeping genes fusA, leuS, pyrG, rpoB and recN genes, showed that the two strains formed a distinct phylogenetic lineage within the Citrobacter genus. Average nucleotide identity (ANI) between strains 97/79T and A121 was 99.2%, whereas ANI values of 97/79T with the type strains of closely related Citrobacter species C...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902227/streptococcus-himalayensis-sp-nov-a-new-streptococcal-species-isolated-from-the-respiratory-tract-of-marmota-himalayana-in-qinghai-tibet-plateau-of-china
#10
Lina Niu, Shan Lu, Xin-He Lai, Shoukui Hu, Cuixia Chen, Gui Zhang, Jing Yang, Dong Jin, Yi Wang, Ruiting Lan, Gang Lu, Yingping Xie, Changyun Ye, Jianguo Xu
Five strains of Gram-positive staining, catalase-negative, coccus-shaped chain-forming organisms separately isolated from the respiratory tracts of 5 Marmota himalayana animals in the Qinghai-Tibet Plateau of China were subjected to phenotypic and molecular taxonomic analyses. Comparative analysis of the 16S rRNA gene indicated that these singular organisms represent a new member of the genus Streptococcus, being phylogenetically closest to Streptococcus marmotae DSM101995T (98.4% similarity). The groEL, sodA and rpoB sequence analysis showed interspecies similarity values between HTS2T and Streptococcus marmotae DSM101995T, its closest 16S rRNA phylogenetic relative, of 98...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902217/microvirga-ossetica-sp-nov-a-new-species-of-rhizobia-isolated-from-root-nodules-of-the-legume-species-vicia-alpestris-steven-originated-from-north-ossetia-region-in-the-caucasus
#11
Vera Safronova, Irina Kuznetsova, Anna Sazanova, Andrey Belimov, Evgeny Andronov, Elizaveta Chirak, Yuri Osledkin, Olga Onishchuk, Oksana Kurchak, Alexander Shaposhnikov, Anne Willems, Igor Tikhonovich
Gram-negative, rod-shaped strains V5/3MT, V5/5K, V5/5M and V5/13 with a medium growth rate were isolated from root nodules of Vicia alpestris plants growing in the North Ossetia region (Caucasus). The sequencing of the partial 16S rDNA gene (rrs) and four housekeeping genes (dnaK, gyrB, recA and rpoB ) showed that the isolates from V. alpestris were most closely related to the species Microvirga zambiemsis (order Rhizobiales, family Methylobacteriaceae) which was described for the single isolate from root nodule of Listia angolensis growing in Zambia...
October 16, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27895008/de-novo-emergence-of-genetically-resistant-mutants-of-mycobacterium-tuberculosis-from-the-persistence-phase-cells-formed-against-antituberculosis-drugs-in-vitro
#12
Jees Sebastian, Sharmada Swaminath, Rashmi Ravindran Nair, Kishor Jakkala, Atul Pradhan, Parthasarathi Ajitkumar
Bacterial persisters are a subpopulation of cells that can tolerate lethal concentrations of antibiotics. However, the possibility of the emergence of genetically resistant mutants from antibiotic persister cell population, upon continued exposure to lethal concentrations of antibiotics, remained unexplored. In the present study, we found that Mycobacterium tuberculosis (Mtb) cells exposed continuously to lethal concentrations of rifampicin (RIF) or moxifloxacin (MXF) for prolonged durations showed killing, RIF/MXF persistence, and regrowth phases...
November 28, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27891120/two-major-clades-of-bradyrhizobia-dominate-symbiotic-interactions-with-pigeonpea-in-fields-of-c%C3%A3-te-d-ivoire
#13
Romain K Fossou, Dominik Ziegler, Adolphe Zézé, François Barja, Xavier Perret
In smallholder farms of Côte d'Ivoire, particularly in the northeast of the country, Cajanus cajan (pigeonpea) has become an important crop because of its multiple beneficial facets. Pigeonpea seeds provide food to make ends meet, are sold on local markets, and aerial parts serve as forage for animals. Since it fixes atmospheric nitrogen in symbiosis with soil bacteria collectively known as rhizobia, C. cajan also improves soil fertility and reduces fallow time. Yet, seed yields remain low mostly because farmers cannot afford chemical fertilizers...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27889135/novel-pcr-rflp-system-based-on-rpob-gene-for-differentiation-of-cronobacter-species
#14
Jiří Vlach, Barbora Javůrková, Ludmila Karamonová, Martina Blažková, Ladislav Fukal
Bacteria from the genus Cronobacter are opportunistic foodborne pathogens that can cause severe infections. More rapid, cost-effective and reliable methods are still required for the species identification of Cronobacter spp. In this study, we present a novel PCR-RFLP-based method that uses a newly designed pair of primers for the PCR-amplification of a partial rpoB gene sequence (1635 bp). The amplified products of DNA from 80 Cronobacter strains were separately digested with three restriction endonucleases (Csp6I, HinP1I, MboI)...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27882322/identification-of-species-of-nontuberculous-mycobacteria-clinical-isolates-from-8-provinces-of-china
#15
Haican Liu, Lulu Lian, Yi Jiang, Mingxiang Huang, Yunhong Tan, Xiuqin Zhao, Jingrui Zhang, Qin Yu, Jiao Liu, Haiyan Dong, Bing Lu, Yimou Wu, Kanglin Wan
Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR, rpoB and hsp65 PCR-PRA, hsp65, rpoB, and 16S-23S internal transcribed spacer region sequencing...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27865235/role-of-real-time-pcr-for-detection-of-tuberculosis-and-drug-resistance-directly-from-clinical-samples
#16
Parashuram Rao, Kiran Chawla, Vishnu Prasad Shenoy, Chiranjay Mukhopadhyay
BACKGROUND: Only a few studies done earlier in India reveal the utility of real-time PCR in detecting drug resistance in cases of pulmonary tuberculosis. OBJECTIVES: The study was carried out to standardise real-time PCR (Quantitative real-time PCR, qPCR) targeting 16s RNA for the rapid detection of tuberculosis and its drug resistance from suspected TB patients. MATERIALS AND METHODS: Sputum samples from 100 clinically suspected tuberculosis patients, after processing were subjected to microscopy, MGIT culture and qPCR...
July 2016: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/27819481/characterization-of-klebsiella-sp-strain-s1-a-bacterial-producer-of-secoisolariciresinol-through-biotransformation
#17
Yu-Jie Zhou, Songling Zhu, Dong-Hui Yang, Dan-Dan Zhao, Jia-Jing Li, Shu-Lin Liu
Secoisolariciresinol (SECO) is a lignan of potential therapeutic value for diseases such as cancer, but its use has been limited by the lack of ideal production methods, even though its precursors are abundant in plants, such as flaxseeds. Here, we report the characterization of a bacterial strain, S1, isolated from the human intestinal flora, which could produce secoisolariciresinol by biotransformation of precursors in defatted flaxseeds. This bacterium was a Gram-negative and facultatively anaerobic straight rod without capsules...
June 29, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27810467/mycobacterium-tuberculosis-drug-resistance-testing-challenges-recent-developments-and-perspectives
#18
REVIEW
T Schön, P Miotto, C U Köser, M Viveiros, E Böttger, E Cambau
Drug-resistance testing, or antimicrobial susceptibility testing (AST), is mandatory for Mycobacterium tuberculosis in cases of failure on standard therapy. We reviewed the different methods and techniques of phenotypic and genotypic approaches. Although multiresistant and extensively drug-resistant (MDR/XDR) tuberculosis is present worldwide, AST for M. tuberculosis (AST-MTB) is still mainly performed according to the resources available rather than the drug-resistance rates. Phenotypic methods, i.e. culture-based AST, are commonly used in high-income countries to confirm susceptibility of new cases of tuberculosis...
November 1, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27793689/phylogenetic-analysis-of-the-pathogenic-genus-aeromonas-spp-isolated-from-diseased-eels-in-china
#19
S L Guo, Q H Yang, J J Feng, L H Duan, J P Zhao
Analyses of 16S rRNA and housekeeping genes (HKGs) were valuated as identification markers for pathogenic Aeromonas isolated from diseased eels. The interrelationships of 32 Aeromonas strains which had been verified as pathogens to eels were studied using phylogenetic analysis with 16S rRNA and HKG sequences (cpn60, gyrB, rpoB and dnaJ) and identified by Biolog automatic microbiology analysis system (gene III). From the analysis of 5 genes, the mean gene divergences of 16S rRNA, cpn60, gyrB, rpoB and dnaJ in 32 isolates were 1...
October 25, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27790199/molecular-events-for-promotion-of-vancomycin-resistance-in-vancomycin-intermediate-staphylococcus-aureus
#20
REVIEW
Qiwen Hu, Huagang Peng, Xiancai Rao
Vancomycin has been used as the last resort in the clinical treatment of serious Staphylococcus aureus infections. Vancomycin-intermediate S. aureus (VISA) was discovered almost two decades ago. Aside from the vancomycin-intermediate phenotype, VISA strains from the clinic or laboratory exhibited common characteristics, such as thickened cell walls, reduced autolysis, and attenuated virulence. However, the genetic mechanisms responsible for the reduced vancomycin susceptibility in VISA are varied. The comparative genomics of vancomycin-susceptible S...
2016: Frontiers in Microbiology
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