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https://www.readbyqxmd.com/read/28641402/ec-tagging-allows-cell-type-specific-rna-analysis
#1
Naoki Hida, Mohamed Y Aboukilila, Dana A Burow, Rakesh Paul, Marc M Greenberg, Michael Fazio, Samantha Beasley, Robert C Spitale, Michael D Cleary
Purification of cell type-specific RNAs remains a significant challenge. One solution involves biosynthetic tagging of target RNAs. RNA tagging via incorporation of 4-thiouracil (TU) in cells expressing transgenic uracil phosphoribosyltransferase (UPRT), a method known as TU-tagging, has been used in multiple systems but can have limited specificity due to endogenous pathways of TU incorporation. Here, we describe an alternative method that requires the activity of two enzymes: cytosine deaminase (CD) and UPRT...
June 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28641399/compannotate-a-comparative-approach-to-annotate-base-pairing-interactions-in-rna-3d-structures
#2
Shahidul Islam, Ping Ge, Shaojie Zhang
The analysis of RNA tertiary structure is hindered by the fact that not too many structural data are available and a significant amount of them are in low resolution. Due to the atomic coordinate errors posed by the limitations of low-resolution RNA three-dimensional structures, it becomes a critical challenge to extract key geometric characteristics of RNA, particularly, the interaction of bases. To address this issue, we have devised a comparative method, named CompAnnotate, that utilizes more precise structural information of high-resolution homologs to annotate the base-pairing interactions in the low-resolution structures, by aligning and making comparative geometric assessments...
June 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28641383/neuroprotection-in-stroke-the-importance-of-collaboration-and-reproducibility
#3
Ain A Neuhaus, Yvonne Couch, Gina Hadley, Alastair M Buchan
Acute ischaemic stroke accounts for 6.5 million deaths per year, and by 2030 will result in the annual loss of over 200 million disability-adjusted life years globally. There have been considerable recent advances in the gold standard of acute ischaemic stroke treatment, some aspects of which-aspirin to prevent recurrence, and treating patients in specialized stroke wards-are widely applicable. Recanalization of the occluded artery through thrombolysis and/or endovascular thrombectomy is restricted to only a small proportion of patients, due to contra-indications and the costs associated with establishing the infrastructure to deliver these treatments...
June 20, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/28641371/effects-of-changes-in-leaf-properties-mediated-by-methyl-jasmonate-meja-on-foliar-absorption-of-zn-mn-and-fe
#4
Cui Li, Peng Wang, Neal W Menzies, Enzo Lombi, Peter M Kopittke
Background and Aims: Foliar fertilization to overcome nutritional deficiencies is becoming increasingly widespread. However, the processes of foliar nutrient absorption and translocation are poorly understood. The present study aimed to investigate how cuticular leaf properties affect the absorption of foliar-applied nutrients in leaf tissues. Methods: Given that methyl jasmonate (MeJA) can cause alterations in leaf properties, we applied 1 m m MeJA to sunflower ( Helianthus annuus ), tomato ( Solanum lycopersicum ) and soybean ( Glycine max ) to assess changes in leaf properties...
June 21, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28641360/-strategies-on-the-treatment-of-nerve-injuries-accompanied-by-severe-soft-tissue-damage-consensus-statement-of-the-german-speaking-society-for-microsurgery-of-peripheral-nerves-and-vessels
#5
Leila Harhaus, Christoph Hirche, Riccardo E Giunta, Oskar Aszmann, Frank Siemers, U Kneser, Marcus Lehnhardt
Nerve injuries with severe soft tissue damage are rare and demand a complex and individualized treatment approach, in order to limit medical and socioeconomic consequences. Since uniform systematic evidence so far is lacking, inconsistent therapy standards are implemented depending on individual experiences and background of respective microsurgeons.In order to provide best conditions for further healing, basic expert consensus statements were developed in a workshop on the 38th Annual Meeting of the German-speaking Community for Microsurgery of the Peripheral Nerves and Vessels (DAM)...
June 22, 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/28641354/-acute-liver-failure-acute-on-chronic-liver-failure-hepatorenal-syndrome-hepatopulmonary-syndrome-and-portopulmonary-hypertension-artificial-liver-support-on-the-icu
#6
Uwe Lodes, David Jacob, Frank Meyer
Background Acute hepatic dysfunction in the form of acute liver failure (ALF) or acute-on-chronic liver failure (ACLF) is a disease with a high risk of mortality and requires interdisciplinary intensive care. Aim This article explains the nomenclature, pathophysiology, prognosis and possible treatment options of ALF and ACLF, including the possibilities of extracorporeal liver support therapy at the point of liver transplantation (LTx). Method Narrative review with a selective literature review and representative case studies...
June 2017: Zentralblatt Für Chirurgie
https://www.readbyqxmd.com/read/28641350/-laparoscopic-assisted-mobilisation-and-resection-of-a-sacrococcygeal-teratoma-altman-type-iii
#7
Jan Gödeke, Veronika Engel, Oliver Münsterer
Objective This video is a step-by-step illustration of the laparoscopic-assisted mobilisation and resection of a sacrococcygeal teratoma Altman type III. Indication Laparoscopic supralevatory tumour mobilisation is an established method in selected centres and can be the initial step in approaching sacrococcygeal teratomas with an intrapelvic portion, facilitating a complete (R0) resection. Method Laparoscopic surgery is performed in supine position with a 5 mm umbilical trocar, a 5 mm trocar in the right upper abdomen, and two 3 mm trocars in the left middle and lower abdomen...
June 2017: Zentralblatt Für Chirurgie
https://www.readbyqxmd.com/read/28641331/-surgical-treatment-of-pseudotumours-of-the-lung
#8
Zsolt Sziklavari, Annete Droste, Reiner Neu, Hans-Stefan Hofmann, Michael Ried
Introduction Pseudotumour of the lung is a collective term for various subentities. Some subgroups are considered to be intermediary malignant tumours. A pseudotumour is a rare condition, which makes it difficult to estimate its incidence and prevalence. Methods Retrospective analysis of all surgically treated patients between 2008 and 2015 diagnosed with a pseudotumour of the lung. The primary endpoint of this study was to estimate the rates of local recurrence and metastasis. Secondary endpoints were to determine the nomenclature, medical history, treatment, and the perioperative course...
June 22, 2017: Zentralblatt Für Chirurgie
https://www.readbyqxmd.com/read/28641322/scuba-diving-and-otology-a-systematic-review-with-recommendations-on-diagnosis-treatment-and-post-operative-care
#9
REVIEW
Devon M Livingstone, Kristine A Smith, Beth Lange
Scuba diving is a popular recreational and professional activity with inherent risks. Complications related to barotrauma and decompression illness can pose significant morbidity to a diver's hearing and balance systems. The majority of dive-related injuries affect the head and neck, particularly the outer, middle and inner ear. Given the high incidence of otologic complications from diving, an evidence-based approach to the diagnosis and treatment of otic pathology is a necessity. We performed a systematic and comprehensive literature review including the pathophysiology, diagnosis, and treatment of otologic pathology related to diving...
June 2017: Diving and Hyperbaric Medicine: the Journal of the South Pacific Underwater Medicine Society
https://www.readbyqxmd.com/read/28641312/impact-of-novel-mir-145-3p-regulatory-networks-on-survival-in-patients-with-castration-resistant-prostate-cancer
#10
Yusuke Goto, Akira Kurozumi, Takayuki Arai, Nijiro Nohata, Satoko Kojima, Atsushi Okato, Mayuko Kato, Kazuto Yamazaki, Yasuo Ishida, Yukio Naya, Tomohiko Ichikawa, Naohiko Seki
BACKGROUND: Despite recent advancements, metastatic castration-resistant prostate cancer (CRPC) is not considered curative. Novel approaches for identification of therapeutic targets of CRPC are needed. METHODS: Next-generation sequencing revealed 945-1248 miRNAs from each lethal mCRPC sample. We constructed miRNA expression signatures of CRPC by comparing the expression of miRNAs between CRPC and normal prostate tissue or hormone-sensitive prostate cancer (HSPC)...
June 22, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28641288/the-crista-fenestra-and-its-impact-on-the-surgical-approach-to-the-scala-tympani-during-cochlear-implantation
#11
Roberto D Angeli, Joel Lavinsky, Enio T Setogutti, Luiz Lavinsky
OBJECTIVE: The aim of this work was to describe the dimensions of the crista fenestra and determine its presence by means of high-resolution computed tomography (CT) for the purpose of cochlear implantation via the round window approach. METHODS: A series of 10 adult human temporal bones underwent high-resolution CT scanning and were further dissected for microscopic study of the round window niche. RESULTS: In all of the specimens, the round window membrane was fully visualized after the complete removal of bony overhangs...
June 23, 2017: Audiology & Neuro-otology
https://www.readbyqxmd.com/read/28641283/-healing-of-amyotrophic-lateral-sclerosis-a-case-report
#12
Inge Mangelsdorf, Harald Walach, Joachim Mutter
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a devastating disease leading to death within 3-5 years in most cases. New approaches to treating this disease are needed. Here, we report a successful therapy. CASE REPORT: In a 49-year-old male patient suffering from muscle weakness and fasciculations, progressive muscular atrophy, a variant of ALS, was diagnosed after extensive examinations ruling out other diseases. Due to supposed mercury exposure from residual amalgam, the patient's teeth were restored...
June 12, 2017: Complementary Medicine Research
https://www.readbyqxmd.com/read/28641276/atherosclerosis-and-the-hydrogen-sulfide-signaling-pathway-therapeutic-approaches-to-disease-prevention
#13
Zhi-Jun Wang, Jian Wu, Wei Guo, Yi-Zhun Zhu
Hydrogen sulfide (H2S) is now admitted as a third gasotransmitter together with nitric oxide (NO) and carbon monoxide (CO), albeit it was originally considered as a foul and poisonous gas. Endogenous H2S production in mammalian cells is counting on the three enzymes acting on cysteine. Involvement of H2S in various physiological and pathological processes has been extensively studied in the last fifteen years. Mounting evidence suggests that H2S is able to protect against atherosclerosis development and progression...
June 23, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28641275/a-case-series-of-surgically-diagnosed-idiopathic-aortitis-in-a-canadian-centre-a-retrospective-study
#14
Diane L Murzin, Eric C Belanger, John P Veinot, Nataliya Milman
BACKGROUND: Idiopathic aortitis became recognized relatively recently, and the body of knowledge concerning this condition is scarce. We aimed to determine the frequency of idiopathic aortitis in aortic specimens, the clinical, laboratory and radiologic characteristics at diagnosis and during follow-up, and the approach to investigation, treatment and monitoring taken by the treating physicians. METHODS: We identified cases of aortitis diagnosed on pathological specimens of the aorta between Jan...
June 19, 2017: CMAJ Open
https://www.readbyqxmd.com/read/28641273/learning-and-recognition-of-clothing-genres-from-full-body-images
#15
Shintami C Hidayati, Chuang-Wen You, Wen-Huang Cheng, Kai-Lung Hua
According to the theory of clothing design, the genres of clothes can be recognized based on a set of visually differentiable style elements, which exhibit salient features of visual appearance and reflect high-level fashion styles for better describing clothing genres. Instead of using less-discriminative low-level features or ambiguous keywords to identify clothing genres, we proposed a novel approach for automatically classifying clothing genres based on the visually differentiable style elements. A set of style elements, that are crucial for recognizing specific visual styles of clothing genres, were identified based on the clothing design theory...
June 19, 2017: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28641266/an-approach-to-identifying-phenomena-accompanying-micro-and-nanoparticles-in-contact-with-irregular-vessel-walls
#16
K Talaska, A Ferreira
OBJECTIVE: The objective of this manuscript is to present the method for determining the nature and values of the forces needed to set micro and nanoparticles sitting immobile at the blood vessel wall in motion. METHODS: The problem was tackled in two ways. Microparticles were examined as objects coming into contact with the wall with the actual large arteriole-type vessel structure. The forces acting on microparticles 10 μm, 30 μm and 50 μm in diameter were determined: drag force FD, lift force FL, electrostatic force FE and gravity force FG...
June 19, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28641261/robust-web-image-annotation-via-exploring-multi-facet-and-structural-knowledge
#17
Mengqiu Hu, Yang Yang, Fumin Shen, Luming Zhang, Heng Tao Shen, Xuelong Li
Driven by the rapid development of Internet and digital technologies, we have witnessed the explosive growth of Web images in recent years. Seeing that labels can reflect the semantic contents of the images, automatic image annotation, which can further facilitate the procedure of image semantic indexing, retrieval and other image management tasks, has become one of the most crucial research directions in multimedia. Most of the existing annotation methods heavily rely on well-labeled training data (expensive to collect) and/or single view of visual features (insufficient representative power)...
June 19, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28641257/fast-segmentation-from-blurred-data-in-3d-fluorescence-microscopy
#18
Martin Storath, Dennis Rickert, Michael Unser, Andreas Weinmann
We develop a fast algorithm for segmenting 3D images from linear measurements based on the Potts model (or piecewise constant Mumford-Shah model). To that end, we first derive suitable space discretizations of the 3D Potts model which are capable of dealing with 3D images defined on non-cubic grids. Our discretization allows us to utilize a specific splitting approach which results in decoupled subproblems of moderate size. The crucial point in the 3D setup is that the number of independent subproblems is so large that we can reasonably exploit the parallel processing capabilities of the graphics processing units (GPU)...
June 16, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28641254/sparse-image-reconstruction-on-the-sphere-analysis-and-synthesis
#19
Christopher G R Wallis, Yves Wiaux, Jason D McEwen
We develop techniques to solve ill-posed inverse problems on the sphere by sparse regularisation, exploiting sparsity in both axisymmetric and directional scale-discretised wavelet space. Denoising, inpainting, and deconvolution problems, and combinations thereof, are considered as examples. Inverse problems are solved in both the analysis and synthesis settings, with a number of different sampling schemes. The most effective approach is that with the most restricted solution-space, which depends on the interplay between the adopted sampling scheme, the selection of the analysis/synthesis problem, and any weighting of the ℓ1 norm appearing in the regularisation problem...
June 16, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28641252/an-embedded-marked-point-process-framework-for-three-level-object-population-analysis
#20
Csaba Benedek
In this paper we introduce a probabilistic approach for extracting complex hierarchical object structures from digital images used by various vision applications. The proposed framework extends conventional Marked Point Process (MPP) models by (i) admitting object-subobject ensembles in parent-child relationships and (ii) allowing corresponding objects to form coherent object groups, by a Bayesian segmentation of the population. Different from earlier, highly domain specific attempts on MPP generalization, the proposed model is defined at an abstract level, providing clear interfaces for applications in various domains...
June 15, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
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