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Serology in dengue

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https://www.readbyqxmd.com/read/28722594/fatal-leptospira-spp-zika-virus-coinfection-puerto-rico-2016
#1
Paige Neaterour, Aidsa Rivera, Renee L Galloway, Myriam Garcia Negrón, Brenda Rivera-Garcia, Tyler M Sharp
Coinfection with pathogens that cause acute febrile illness (AFI) can complicate patient diagnosis and management. This report describes a fatal case of Leptospira spp./Zika virus (ZIKV) coinfection in Puerto Rico. The patient presented with a 5-day history of AFI; reported behavioral risk factors for leptospirosis; was diagnosed with possible leptospirosis, dengue, chikungunya, or ZIKV disease; and received appropriate treatment of leptospirosis and dengue. Following a 3-day hospitalization, the patient died due to acute gastrointestinal hemorrhage, and kidney and liver failure...
June 26, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28716913/antibody-based-assay-discriminates-zika-virus-infection-from-other-flaviviruses
#2
Angel Balmaseda, Karin Stettler, Raquel Medialdea-Carrera, Damaris Collado, Xia Jin, José Victor Zambrana, Stefano Jaconi, Elisabetta Cameroni, Saira Saborio, Francesca Rovida, Elena Percivalle, Samreen Ijaz, Steve Dicks, Ines Ushiro-Lumb, Luisa Barzon, Patricia Siqueira, David W G Brown, Fausto Baldanti, Richard Tedder, Maria Zambon, A M Bispo de Filippis, Eva Harris, Davide Corti
Zika virus (ZIKV) is a mosquito-borne flavivirus that emerged recently as a global health threat, causing a pandemic in the Americas. ZIKV infection mostly causes mild disease, but is linked to devastating congenital birth defects and Guillain-Barré syndrome in adults. The high level of cross-reactivity among flaviviruses and their cocirculation has complicated serological approaches to differentially detect ZIKV and dengue virus (DENV) infections, accentuating the urgent need for a specific and sensitive serological test...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28706387/comparison-of-nonstructural-protein-1-antigen-detection-by-rapid-and-enzyme-linked-immunosorbent-assay-test-and-its-correlation-with-polymerase-chain-reaction-for-early-diagnosis-of-dengue
#3
Seema Gaikwad, Sandhya S Sawant, Jayanthi S Shastri
INTRODUCTION: Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-time polymerase chain reaction (RT-PCR) for diagnosis of dengue...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28700062/seropositivity-for-rickettsia-spp-and-ehrlichia-spp-in-the-human-population-of-mato-grosso-central-western-brazil
#4
Maria Cristina Fuzari Bezerra, Andréia Lima Tomé Melo, Isis Indaiara Gonçalves Granjeiro Taques, Daniel Moura de Aguiar, Richard Campos Pacheco, Renata Dezengrini Slhessarenko
INTRODUCTION: The epidemiology of Rickettsia and Ehrlichia species infection is underestimated in Mato Grosso State. METHODS: Serum samples obtained during a Dengue outbreak in 2011-2012 were tested via indirect immunofluorescence and/or ELISA. RESULTS: Samples from 19/506 (3.8%) patients presented antibodies for at least one of three Rickettsia species; 2/506 (0.4%) samples reacted against Ehrlichia canis. Most afflicted patients are residents of cities from the south-central region of the state, where these diseases have been reported in animals...
May 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28699570/camino-verde-the-green-way-evidence-based-community-mobilisation-for-dengue-control-in-nicaragua-and-mexico-feasibility-study-and-study-protocol-for-a-randomised-controlled-trial
#5
Neil Andersson, Jorge Arostegui, Elizabeth Nava-Aguilera, Eva Harris, Robert J Ledogar
BACKGROUND: Since the Aedes aegypti mosquitoes that transmit dengue virus can breed in clean water, WHO-endorsed vector control strategies place sachets of organophosphate pesticide, temephos (Abate), in household water storage containers. These and other pesticide-dependent approaches have failed to curb the spread of dengue and multiple dengue virus serotypes continue to spread throughout tropical and subtropical regions worldwide. A feasibility study in Managua, Nicaragua, generated instruments, intervention protocols, training schedules and impact assessment tools for a cluster randomised controlled trial of community-based approaches to vector control comprising an alternative strategy for dengue prevention and control in Nicaragua and Mexico...
May 30, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28699562/which-green-way-description-of-the-intervention-for-mobilising-against-aedes-aegypti-under-difficult-security-conditions-in-southern-mexico
#6
REVIEW
Arcadio Morales-Perez, Elizabeth Nava-Aguilera, José Legorreta-Soberanis, Sergio Paredes-Solís, Alejandro Balanzar-Martínez, Felipe René Serrano-de Los Santos, Claudia Erika Ríos-Rivera, Jaime García-Leyva, Robert J Ledogar, Anne Cockcroft, Neil Andersson
BACKGROUND: Community mobilisation for prevention requires engagement with and buy in from those communities. In the Mexico state of Guerrero, unprecedented social violence related to the narcotics trade has eroded most community structures. A recent randomised controlled trial in 90 coastal communities achieved sufficient mobilisation to reduce conventional vector density indicators, self-reported dengue illness and serologically proved dengue virus infection. METHODS: The Camino Verde intervention was a participatory research protocol promoting local discussion of baseline evidence and co-design of vector control solutions...
May 30, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28699560/dengue-occurrence-relations-and-serology-cross-sectional-analysis-of-results-from-the-guerrero-state-mexico-baseline-for-a-cluster-randomised-controlled-trial-of-community-mobilisation-for-dengue-prevention
#7
Elizabeth Nava-Aguilera, Arcadio Morales-Pérez, Alejandro Balanzar-Martínez, Ofelia Rodríguez-Ramírez, Abel Jiménez-Alejo, Miguel Flores-Moreno, David Gasga-Salinas, José Legorreta-Soberanis, Sergio Paredes-Solís, Pedro Antonio Morales-Nava, María de Lourdes Soto-Ríos, Robert J Ledogar, Joséfina Coloma, Eva Harris, Neil Andersson
BACKGROUND: The Mexican arm of the Camino Verde trial of community mobilisation for dengue prevention covered three coastal regions of Guerrero state: Acapulco, Costa Grande and Costa Chica. A baseline cross-sectional survey provided data for community mobilisation and for adapting the intervention design to concrete conditions in the intervention areas. METHODS: Trained field teams constructed community profiles in randomly selected clusters, based on observation and key informant interviews...
May 30, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28699557/-where-we-put-little-fish-in-the-water-there-are-no-mosquitoes-a-cross-sectional-study-on-biological-control-of-the-aedes-aegypti-vector-in-90-coastal-region-communities-of-guerrero-mexico
#8
Arcadio Morales-Pérez, Elizabeth Nava-Aguilera, José Legorreta-Soberanis, Antonio Juan Cortés-Guzmán, Alejandro Balanzar-Martínez, Eva Harris, Josefina Coloma, Víctor M Alvarado-Castro, Mónica Violeta Bonilla-Leon, Liliana Morales-Nava, Robert J Ledogar, Anne Cockcroft, Neil Andersson
BACKGROUND: In the Mexican state of Guerrero, some households place fish in water storage containers to prevent the development of mosquito larvae. Studies have shown that larvivorous fish reduce larva count in household water containers, but there is a lack of evidence about whether the use of fish is associated with a reduction in dengue virus infection. We used data from the follow up survey of the Camino Verde cluster randomised controlled trial of community mobilisation to reduce dengue risk to study this association...
May 30, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28699541/randomised-controlled-trials-and-changing-public-health-practice
#9
EDITORIAL
Anne Cockcroft
One reason for doing randomised controlled trials (RCTs) is that experiments can be convincing. Early epidemiological experimenters, such as Jenner and the smallpox vaccine and Snow and his famous Broad Street pump handle, already knew the answer they were demonstrating; they used the experiments as knowledge translation devices to convince others.More sophisticated modern experiments include cluster randomised controlled trials (CRCTs) for experiments in the public health setting. The knowledge translation value remains: RCTs and CRCTs can potentially stimulate changes of practice among stakeholders...
May 30, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28673021/emergence-of-west-nile-virus-in-west-bengal-india-a-new-report
#10
Tanuja Khatun, Shyamalendu Chatterjee
Background: The ICMR virus unit in Kolkata functions as an Appex Referral Laboratory for the detection of dengue (DENV) and chikungunya (CHIKV) infections in the eastern part of India. In spite of efforts for confirmatory diagnosis, some samples remain undiagnosed every year. West Nile virus (WNV) infection may mimic either dengue (flavivirus) or chikungunya (alphavirus) like illness. WNV is endemic in the tropical region where its principal/potential vectors are Aedes and Culex. Methods: We explored the existence of WNV within undiagnosed samples to identify the emergence of a new public health problem...
April 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28662144/seroprevalence-of-antibodies-to-dengue-and-chikungunya-viruses-in-thailand
#11
Sompong Vongpunsawad, Duangnapa Intharasongkroh, Thanunrat Thongmee, Yong Poovorawan
The abundance of Aedes mosquito species enabled widespread transmission of mosquito-borne chikungunya virus (CHIKV) and dengue virus (DENV) in Southeast Asia. Periodic seroprevalence surveys are therefore necessary to assess the viral burden in the population and the effectiveness of public health interventions. Since the current seroprevalence for CHIKV and DENV in Thailand are unknown, we evaluated evidence of past infection among Thais. Eight-hundred and thirty-five serum samples obtained from individuals living in central and southern Thailand were assessed for anti-CHIKV and anti-DENV IgG antibodies using commercial enzyme-linked immunosorbent assays...
2017: PloS One
https://www.readbyqxmd.com/read/28638845/detection-of-the-northeastern-african-rift-valley-fever-virus-lineage-during-the-2015-outbreak-in-mauritania
#12
Ndeye Sakha Bob, Hampâté Bâ, Gamou Fall, Elkhalil Ishagh, Mamadou Y Diallo, Abdourahmane Sow, Pape Mbacké Sembene, Ousmane Faye, Brahim El Kouri, Mohamed Lemine Sidi, Amadou Alpha Sall
BACKGROUND: Rift Valley fever (RVF) is an acute viral anthropozoonosis that causes epizootics and epidemics among livestock population and humans. Multiple emergences and reemergences of the virus have occurred in Mauritania over the last decade. This article describes the outbreak that occurred in 2015 in Mauritania and reports the results of serological and molecular investigations of blood samples collected from suspected RVF patients. METHODS: An RVF outbreak was reported from 14 September to 26 November 2015 in Mauritania...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28620811/serological-molecular-and-clinical-correlates-of-dengue-from-a-tertiary-care-centre-in-chennai-india
#13
Vigna Seshan, Gopalsamy Sarangan, Khaleefathullah Sheriff, Kaveri Krishnasamy, Gunasekaran Palani, Padma Srikanth
Dengue disease is caused by dengue viruses 1-4 and has been ranked by the World Health Organisation (WHO) as the fastest spreading vector-borne viral disease. Dengue is often underreported and misdiagnosed due to a wide spectrum of clinical manifestations. Diagnosis of dengue is based on clinical case definitions and laboratory methods. Newer case definitions of dengue have been formulated by clinical studies in order to improve case detection. Owing to its epidemic potential, mortality and morbidity, there is a need for a rapid and accurate diagnostic assay for dengue in order to help the clinician in the early detection of cases and to prevent disease progression...
June 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28617803/dengue-seroprevalence-and-force-of-primary-infection-in-a-representative-population-of-urban-dwelling-indonesian-children
#14
Ari Prayitno, Anne-Frieda Taurel, Joshua Nealon, Hindra Irawan Satari, Mulya Rahma Karyanti, Rini Sekartini, Soedjatmiko Soedjatmiko, Hartono Gunardi, Bernie Endyarni Medise, R Tedjo Sasmono, James Mark Simmerman, Alain Bouckenooghe, Sri Rezeki Hadinegoro
BACKGROUND: Indonesia reports the second highest dengue disease burden in the world; these data are from passive surveillance reports and are likely to be significant underestimates. Age-stratified seroprevalence data are relatively unbiased indicators of past exposure and allow understanding of transmission dynamics. METHODOLOGY/PRINCIPAL FINDINGS: To better understand dengue infection history and associated risk factors in Indonesia, a representative population-based cross-sectional dengue seroprevalence study was conducted in 1-18-year-old urban children...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28616499/emergence-of-wesselsbron-virus-among-black-rat-and-humans-in-eastern-senegal-in-2013
#15
Moussa M Diagne, Martin Faye, Oumar Faye, Abdourahmane Sow, Fanny Balique, Mbacké Sembène, Laurent Granjon, Pascal Handschumacher, Ousmane Faye, Mawlouth Diallo, Amadou A Sall
Wesselsbron disease is a neglected mosquito transmitted Flavivirus infection that causes abortions and has teratogenic effects on sheep and cattle in Africa. Human can also be infected. The detection of human or animal cases is complicated by the non-specific symptoms close to Rift Valley Fever (RVF) in domestic livestock species or Dengue like syndrome in humans. Then, these detections are usually made during RVF investigations in sheep. These domestic animals should take a role in the life cycle of the virus but some evidences of Wesselsbron virus (WSLV) presence in wild animals suggest that the latter may be involved in the virus maintenance in nature...
June 2017: One Health
https://www.readbyqxmd.com/read/28599678/the-prevalence-of-dengue-virus-serotypes-in-asymptomatic-blood-donors-reveals-the-emergence-of-serotype-4-in-saudi-arabia
#16
Ahmed Mohamed Ashshi
BACKGROUND: Transmission of dengue virus (DENV) through blood transfusion has been documented and hence screening for DENV during blood donation has been recently recommended by the American Association of Blood Banks and Centres of Disease Control and Prevention. DENV is endemic in the Western province of the Kingdom of Saudi Arabia (KSA) and serotypes 1, 2 and 3, but not 4, have been detected. However, little is known regarding the rates of DENV during blood donation in the kingdom...
June 9, 2017: Virology Journal
https://www.readbyqxmd.com/read/28599640/dengue-type-4-in-rio-de-janeiro-brazil-case-characterization-following-its-introduction-in-an-endemic-region
#17
Manoela Heringer, Thiara Manuele A Souza, Monique da Rocha Q Lima, Priscila Conrado G Nunes, Nieli Rodrigues da C Faria, Fernanda de Bruycker-Nogueira, Thaís Chouin-Carneiro, Rita Maria R Nogueira, Flavia Barreto Dos Santos
BACKGROUND: Due to the populations' susceptibility, DENV-4 introduction in 2010 led to the occurrence of explosive epidemics in the following years in Brazil. In 2011, DENV-4 was identified in Rio de Janeiro (RJ) and it was prevalent in 2012 and 2013. Here, we aimed to characterize clinical, epidemiological and laboratorial aspects of DENV-4 cases after this serotype introduction in an endemic scenario. METHODS: Dengue suspected cases (n = 3727) were received and analyzed from January 2011 to December 2013, during outbreaks occurred in RJ, Brazil...
June 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28575919/zika-virus-infection-in-pregnant-women-and-microcephaly
#18
Geraldo Duarte, Antonio Fernandes Moron, Artur Timerman, César Eduardo Fernandes, Corintio Mariani Neto, Gutemberg Leão de Almeida Filho, Heron Werner Junior, Hilka Flavia Barra do Espírito Santo, João Alfredo Piffero Steibel, João Bortoletti Filho, Juvenal Barreto Borriello de Andrade, Marcelo Burlá, Marcos Felipe Silva de Sá, Newton Eduardo Busso, Paulo César Giraldo, Renato Augusto Moreira de Sá, Renato Passini Junior, Rosiane Mattar, Rossana Pulcineli Vieira Francisco
From the discovery of the Zika virus (ZIKV) in 1947 in Uganda (Africa), until its arrival in South America, it was not known that it would affect human reproductive life so severely. Today, damage to the central nervous system is known to be multiple, and microcephaly is considered the tip of the iceberg. Microcephaly actually represents the epilogue of this infection's devastating process on the central nervous system of embryos and fetuses. As a result of central nervous system aggression by the ZIKV, this infection brings the possibility of arthrogryposis, dysphagia, deafness and visual impairment...
May 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28562637/identification-of-diagnostic-peptide-regions-that-distinguish-zika-virus-from-related-mosquito-borne-flaviviruses
#19
Alexandra J Lee, Roshni Bhattacharya, Richard H Scheuermann, Brett E Pickett
Zika virus (ZIKV) is a member of the Flavivirus genus of positive-sense single-stranded RNA viruses, which includes Dengue, West Nile, Yellow Fever, and other mosquito-borne arboviruses. Infection by ZIKV can be difficult to distinguish from infection by other mosquito-borne Flaviviruses due to high sequence similarity, serum antibody cross-reactivity, and virus co-circulation in endemic areas. Indeed, existing serological methods are not able to consistently differentiate ZIKV from other Flaviviruses, which makes it extremely difficult to accurately calculate the incidence rate of Zika-associated Guillain-Barre in adults, microcephaly in newborns, or asymptomatic infections within a geographical area...
2017: PloS One
https://www.readbyqxmd.com/read/28562600/effects-of-flood-irrigation-on-the-risk-of-selected-zoonotic-pathogens-in-an-arid-and-semi-arid-area-in-the-eastern-kenya
#20
Bernard Bett, Mohammed Y Said, Rosemary Sang, Salome Bukachi, Salome Wanyoike, Shem C Kifugo, Fredrick Otieno, Enoch Ontiri, Ian Njeru, Johanna Lindahl, Delia Grace
To investigate the effects of irrigation on land cover changes and the risk of selected zoonotic pathogens, we carried out a study in irrigated, pastoral and riverine areas in the eastern Kenya. Activities implemented included secondary data analyses to determine land use and land cover (LULC) changes as well as human, livestock and wildlife population trends; entomological surveys to characterize mosquitoes population densities and species distribution by habitat and season; and serological surveys in people to determine the risk of Rift Valley fever virus (RVFV), West Nile fever virus (WNV), dengue fever virus (DFV), Leptospira spp...
2017: PloS One
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