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Serology in dengue

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https://www.readbyqxmd.com/read/28306183/flavivirus-cross-reactivity-in-serological-tests-and-guillain-barr%C3%A3-syndrome-in-a-hematopoietic-stem-cell-transplant-patient-a-case-report
#1
Sonia M Raboni, Carmem Bonfim, Bernardo M Almeida, Camila Zanluca, Andrea C Koishi, Paula R V P Rodrigues, Claudia K Kay, Lisandro L Ribeiro, Rosana H Scola, Claudia N Duarte Dos Santos
Serological diagnosis of flavivirus infection is a challenge, particularly in the context of a disease associated with immune response enhancement in a transplant patient, where aspects such as previous flavivirus infections may be involved with the outcome. We report a case of a pediatric patient who developed Guillain-Barré syndrome (GBS) after matched-unrelated hematopoietic stem cell transplantation (HSCT). The patient lives in a Brazilian region that is experiencing an epidemic of Zika virus (ZIKV) and dengue virus (DENV)...
March 17, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28302072/imported-arboviral-infections-in-italy-july-2014-october-2015-a-national-reference-laboratory-report
#2
Claudia Fortuna, Maria Elena Remoli, Caterina Rizzo, Eleonora Benedetti, Cristiano Fiorentini, Antonino Bella, Claudio Argentini, Francesca Farchi, Concetta Castilletti, Maria Rosaria Capobianchi, Lorenzo Zammarchi, Alessandro Bartoloni, Nadia Zanchetta, Maria Rita Gismondo, Luca Ceccherini Nelli, Giustina Vitale, Franco Baldelli, Pierlanfranco D'Agaro, Giuseppe Sodano, Giovanni Rezza, Giulietta Venturi
BACKGROUND: Imported cases of infections due to Dengue (DENV) and Chikungunya (CHIKV) viruses and, more recently, Zika virus (ZIKV) are commonly reported among travelers returning from endemic regions. In areas where potentially competent vectors are present, the risk of autochthonous transmission of these vector-borne pathogens is relatively high. Laboratory surveillance is crucial to rapidly detect imported cases in order to reduce the risk of transmission. This study describes the laboratory activity performed by the National Reference Laboratory for Arboviruses (NRLA) at the Italian National Institute of Health in the period from July 2014 to October 2015...
March 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28292653/co-circulating-serotypes-in-a-dengue-fever-outbreak-differential-hematological-profiles-and-phylogenetic-relationships-among-viruses
#3
Andreia Moreira Dos Santos Carmo, Rodrigo Buzinaro Suzuki, Aline Diniz Cabral, Renata Torres da Costa, Gabriela Pena Massari, Michele Marcondes Riquena, Helio Augusto Alves Fracasso, Andre Eterovic, Arlei Marcili, Márcia Aparecida Sperança
BACKGROUND: Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília. OBJECTIVES: The aim of this article was to distinguish dengue serotypes circulating during the 2007 Marília outbreak and define their association to demographic and hematological patient profiles, as well as the phylogenetic relationships among the different viruses...
March 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28284914/dengue-virus-serologic-prevalence-and-seroconversion-rates-in-children-and-adults-in-medellin-colombia-implications-for-vaccine-introduction
#4
Mabel Carabali, Jacqueline Kyungah Lim, Diana Carolina Velez, Andrea Trujillo, Jorge Egurrola, Kang Sung Lee, Jay S Kaufman, Luiz Jacinto DaSilva, Ivan Dario Velez, Jorge E Osorio
BACKGROUND: Dengue is an important public health problem worldwide. Recently a vaccine has been licensed in some countries of Latin America and Asia. Recommendations for dengue vaccine introduction include endemicity and high serologic prevalence of dengue in the territories considering its introduction. METHODS: We conducted a community-based survey to estimate dengue seroprevalence and age-specific seroconversion rates in a community in Medellin, Colombia, using dengue serologic test (IgG Indirect ELISA)...
March 8, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28283425/a-rapid-zika-diagnostic-assay-to-measure-neutralizing-antibodies-in-patients
#5
Chao Shan, Xuping Xie, Ping Ren, Michael J Loeffelholz, Yujiao Yang, Andrea Furuya, Alan P Dupuis, Laura D Kramer, Susan J Wong, Pei-Yong Shi
The potential association of microcephaly and other congenital abnormalities with Zika virus (ZIKV) infection during pregnancy underlines the critical need for a rapid and accurate diagnosis. Due to the short duration of ZIKV viremia in infected patients, a serologic assay that detects antibody responses to viral infection plays an essential role in diagnosing patient specimens. The current serologic diagnosis of ZIKV infection relies heavily on the labor-intensive Plaque Reduction Neutralization Test (PRNT) that requires more than one-week turnaround time and represents a major bottleneck for patient diagnosis...
March 4, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28280696/dengue-in-renal-transplant-recipients-clinical-course-and-impact-on-renal-function
#6
Paula Frassinetti Castelo Branco Camurça Fernandes, Reed André Siqueira, Evelyne Santana Girão, Rainne André Siqueira, Márcia Uchoa Mota, Leyla Castelo Branco Fernandes Marques, Silvana Cristina Albuquerque Andrade, Wilson Mendes Barroso, Sônia Leite Silva, Bruno Gomes Rodrigues Dos Santos, Claúdia Maria Costa de Oliveira
AIM: To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft function. METHODS: We retrospectively evaluated 11 renal transplant recipients (RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS: Positive dengue serology (IgM) was found in all patients...
February 24, 2017: World Journal of Transplantation
https://www.readbyqxmd.com/read/28274805/expression-refolding-and-bio-structural-analysis-of-a-tetravalent-recombinant-dengue-envelope-domain-iii-protein-for-serological-diagnosis
#7
Maxime Combe, Xavier Lacoux, Jérôme Martinez, Odile Méjan, Françoise Luciani, Soizic Daniel
Dengue is a mosquito-borne disease caused by four genetically and serologically related viruses that affect several millions of people. Envelope domain III (EDIII) of the viral envelope protein contains dengue virus (DENV) type-specific and DENV complex-reactive antigenic sites. Here, we describe the expression in Escherichia coli, the refolding and bio-structural analysis of envelope domain III of the four dengue serotypes as a tetravalent dengue protein (EDIIIT2), generating an attractive diagnostic candidate...
March 6, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28254680/enhancing-the-sensitivity-of-dengue-virus-serotype-detection-by-rt-pcr-among-infected-children-in-india
#8
Syed Fazil Ahamed, Rosario Vivek, Shalini Kotabagi, Kaustuv Nayak, Anmol Chandele, Murali-Krishna Kaja, Anita Shet
Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay)...
February 28, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28228395/antibody-responses-to-zika-virus-infections-in-flavivirus-endemic-environments
#9
Sarah L Keasey, Christine L Pugh, Stig M R Jensen, Jessica L Smith, Robert D Hontz, Anna P Durbin, Dawn M Dudley, David H O'Connor, Robert G Ulrich
Zika virus (ZIKV) infections occur in areas where dengue (DENV), West Nile (WNV), yellow fever (YFV), and other viruses of the genus Flavivirus co-circulate. The envelope protein (E) of these closely related flaviviruses induces specific long-term immunity, yet subsequent infections are associated with cross-reactive antibody responses that may enhance disease susceptibility and severity. To gain a better understanding of ZIKV infections against a background of similar viral diseases, we examined serological immune responses to ZIKV, WNV, DENV and YFV infections of humans and non-human primates (NHP)...
February 22, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28208856/seroprevalence-of-common-viral-diseases-a-hospital-based-study-from-amritsar-india
#10
Kanwardeep Singh, Shailpreet K Sidhu, Pushpa Devi, Manpreet Kaur, Maninder Kaur, Nachhatarjit Singh
INTRODUCTION: There is a paucity of published data on epidemiology of the viral infections in Punjab, India. The Viral Research and Diagnostic Laboratory (VRDL) located at Government Medical College (GMC), Amritsar conducts the initial diagnosis of common viruses circulating in the region. AIM: To report the seroprevalence of various viral diseases in Amritsar and neighboring districts of Punjab along with the demographic and geographical data of the patients. MATERIALS AND METHODS: Whole blood sera samples from a total of 5781 patients suspected of various viral diseases (chikungunya, dengue, hepatitis A, hepatitis B, hepatitis C, hepatitis E, herpes simplex-1 or herpes simplex-2) were received at VRDL, GMC, Amritsar from January 2015 to April 2016 and a cross sectional study was conducted...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28187130/detection-of-the-mosquito-borne-flaviviruses-west-nile-dengue-saint-louis-encephalitis-ilheus-bussuquara-and-yellow-fever-in-free-ranging-black-howlers-alouatta-caraya-of-northeastern-argentina
#11
María A Morales, Cintia M Fabbri, Gabriel E Zunino, Martín M Kowalewski, Victoria C Luppo, Delia A Enría, Silvana C Levis, Gladys E Calderón
Several medically important mosquito-borne flaviviruses have been detected in Argentina in recent years: Dengue (DENV), St. Louis encephalitis (SLEV), West Nile (WNV) and Yellow Fever (YFV) viruses. Evidence of Bussuquara virus (BSQV) and Ilheus virus (ILHV) activity were found, but they have not been associated with human disease. Non-human primates can act as important hosts in the natural cycle of flaviviruses and serological studies can lead to improved understanding of virus circulation dynamics and host susceptibility...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28167601/imported-dengue-infection-in-a-spanish-hospital-with-a-high-proportion-of-travelers-from-africa-a-9-year-retrospective-study
#12
Carlos Toro, Patricia Trevisi, Beatriz López-Quintana, Aránzazu Amor, Nuria Iglesias, Mercedes Subirats, Concepción Ladrón de Guevara, Mar Lago, Marta Arsuaga, Fernando de la Calle-Prieto, Dolores Herrero, Margarita Rubio, Sabino Puente, Margarita Baquero
Epidemiological data on dengue in Africa are still scarce. We investigated imported dengue infection among travelers with a high proportion of subjects from Africa over a 9-year period. From January 2005 to December 2013, blood samples from travelers with clinical suspicion of dengue were analyzed. Dengue was diagnosed using serological, antigen detection, and molecular methods. Subjects were classified according to birthplace (Europeans versus non-Europeans) and last country visited. Overall, 10,307 serum samples corresponding to 8,295 patients were studied; 62% were European travelers, most of them from Spain, and 35...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28148289/a-rare-case-of-dengue-encephalopathy-complicating-a-term-pregnancy
#13
Lavanya Rajagopala, Ravindra L Satharasinghe, Madhava Karunarathna
BACKGROUND: Dengue fever has an expanded clinical spectrum ranging from an asymptomatic infection to life threatening dengue hemorrhagic fever and refractory shock. Dengue infection in pregnancy can be a diagnostic dilemma, particularly considering the physiological changes in pregnancy and the obstetric complications encountered in clinical practice. Hence the knowledge of its diagnosis and management in its atypical presentations is of paramount importance. Here we report an unusual case of uncomplicated dengue encephalopathy in a term mother, probably the first to be reported from the Indian subcontinent...
February 2, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28126366/testing-for-zika-virus-infection-in-pregnancy-key-concepts-to-deal-with-an-emerging-epidemic
#14
REVIEW
Catherine Eppes, Martha Rac, James Dunn, James Versalovic, Kristy O Murray, Melissa A Suter, Magda Sanz Cortes, Jimmy Espinoza, Maxim D Seferovic, Wesley Lee, Peter Hotez, Joan Mastrobattista, Steven L Clark, Michael A Belfort, Kjersti M Aagaard
Zika virus is an emerging mosquito-borne (Aedes genus) arbovirus of the Flaviviridae family. Following epidemics in Micronesia and French Polynesia during the past decade, more recent Zika virus infection outbreaks were first reported in South America as early as May 2013 and spread to now 50 countries throughout the Americas. Although no other flavivirus has previously been known to cause major fetal malformations following perinatal infection, reports of a causal link between Zika virus and microcephaly, brain and ocular malformations, and fetal loss emerged from hard-hit regions of Brazil by October 2015...
March 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28115671/mosquito-exposure-and-chikungunya-and-dengue-infection-among-travelers-during-the-chikungunya-outbreak-in-the-americas
#15
David A Lindholm, Todd Myers, Susana Widjaja, Edward M Grant, Kalyani Telu, Tahaniyat Lalani, Jamie Fraser, Mary Fairchok, Anuradha Ganesan, Mark D Johnson, Anjali Kunz, David R Tribble, Heather C Yun
Travelers are at risk for arbovirus infection. We prospectively enrolled 267 Department of Defense beneficiaries traveling to chikungunya-outbreak regions in the Americas between December 2013 and May 2015 and assessed travel characteristics and serologic exposure to chikungunya virus (CHIKV) and dengue virus (DENV). Ten ill returning travelers were also assessed retrospectively. Self-reported mosquito exposure was common (64% of 198 evaluable travelers saw mosquitoes; 53% of 201 reported ≥ 1 bite). Increased exposure was associated with active-duty travelers (odds ratio [OR] = 2...
January 23, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28107859/dengue-fever-may-mislead-the-surgeons-when-it-presents-as-an-acute-abdomen
#16
Bingumal Jayasundara, Lalith Perera, Ajith de Silva
OBJECTIVE: To review the management experience of a consecutive series of patients presenting as acute surgical abdomen whom were ultimately diagnosed to have DF (Dengue fever)/DHF (Dengue hemorrhagic fever). METHODS: Clinical data of all cases of apparent acute abdomen (AA) which were later confirmed as having DF/DHF reviewed by two surgical units from December 2012 to December 2013 were analyzed. Initially confirmed patients with DF/DHF who developed abdominal symptoms were not considered...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28107858/serodiagnosis-of-asymptomatic-dengue-infection
#17
REVIEW
Supawat Chatchen, Arunee Sabchareon, Chukiat Sirivichayakul
Dengue virus (DENV) is a mosquito-transmitted virus that is expanding across the world. The incidence of dengue infection, especially severe disease, has been increasing. DENV consist of 4 serotypes of single stranded RNA viruses (D1-D4) in the genus Flavivirus, family Flaviviridae. Majority of dengue infections are asymptomatic cases, which cause difficulty in disease control and are important in dengue surveillance. There is still no gold standard to diagnose asymptomatic dengue infection. Plaque reduction neutralization test (PRNT) has been developed for many purposes such as immunological study, clinical study, vaccine trial and is currently the most sensitive and specific method for serological surveillance...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28104506/assessing-the-risk-of-dengue-virus-transmission-in-a-non-endemic-city-surrounded-by-endemic-and-hyperendemic-areas
#18
Tsz Ho Kwan, Shui Shan Lee, Denise Pui Chung Chan, Manton Cheung, Kai Man Kam
OBJECTIVE: To assess the potential risk of dengue transmission in a non-endemic city using a spatial epidemiological approach. METHODS: Past dengue exposure of the general population was examined by dengue virus (DENV) IgG testing of archived samples from voluntary blood donors. Vector intensities were determined by local ovitrap index (OI). Analyses were made in the context of population statistics at both the district and sub-district level. RESULTS: The overall prevalence of DENV IgG was low at 2...
January 16, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28103255/mitigating-diseases-transmitted-by-aedes-mosquitoes-a-cluster-randomised-trial-of-permethrin-impregnated-school-uniforms
#19
Pattamaporn Kittayapong, Phanthip Olanratmanee, Pongsri Maskhao, Peter Byass, James Logan, Yesim Tozan, Valérie Louis, Duane J Gubler, Annelies Wilder-Smith
BACKGROUND: Viral diseases transmitted via Aedes mosquitoes are on the rise, such as Zika, dengue, and chikungunya. Novel tools to mitigate Aedes mosquitoes-transmitted diseases are urgently needed. We tested whether commercially insecticide-impregnated school uniforms can reduce dengue incidence in school children. METHODS: We designed a cluster-randomised controlled trial in Thailand. The primary endpoint was laboratory-confirmed dengue infections. Secondary endpoints were school absenteeism; and impregnated uniforms' 1-hour knock-down and 24 hour mosquito mortality as measured by standardised WHOPES bioassay cone tests at baseline and after repeated washing...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28094237/a-multiplex-microsphere-immunoassay-for-zika-virus-diagnosis
#20
Susan J Wong, Andrea Furuya, Jing Zou, Xuping Xie, Alan P Dupuis, Laura D Kramer, Pei-Yong Shi
Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, and countermeasure development. The present serologic diagnosis of Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which is confounded with the flaw of cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures the diagnostic power of viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses) and the differential power of viral nonstructural proteins NS1 and NS5 (that induce more virus-type specific antibodies)...
February 2017: EBioMedicine
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