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https://www.readbyqxmd.com/read/29214201/genotyping-by-sequencing-data-of-272-crested-wheatgrass-agropyron-cristatum-genotypes
#1
Pingchuan Li, Bill Biligetu, Bruce E Coulman, Michael Schellenberg, Yong-Bi Fu
Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is an important cool-season forage grass widely used for early spring grazing. However, the genomic resources for this non-model plant are still lacking. Our goal was to generate the first set of next generation sequencing data using the genotyping-by-sequencing technique. A total of 272 crested wheatgrass plants representing seven breeding lines, five cultivars and five geographically diverse accessions were sequenced with an Illumina MiSeq instrument. These sequence datasets were processed using different bioinformatics tools to generate contigs for diploid and tetraploid plants and SNPs for diploid plants...
December 2017: Data in Brief
https://www.readbyqxmd.com/read/29137188/physical-localization-of-a-locus-from-agropyron-cristatum-conferring-resistance-to-stripe-rust-in-common-wheat
#2
Zhi Zhang, Liqiang Song, Haiming Han, Shenghui Zhou, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC₂F₂ and BC₃F₂ populations of two wheat-A...
November 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29105256/fusarium-crown-rot-caused-by-fusarium-pseudograminearum-in-cereal-crops-recent-progress-and-future-prospects
#3
Kemal Kazan, Donald Gardiner
Diseases caused by Fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. Fusarium crown rot (FCR) caused by Fusarium pseudograminearum is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. In recent years, this disease has become more prevalent, in part due to the adoption of moisture-preserving cultural practices such as minimum tillage and stubble retention. In this pathogen profile, we present a brief overview of recent research efforts that not only have advanced our understanding of the interactions between F...
November 4, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29093800/diversification-of-the-p-genome-among-agropyron-gaertn-poaceae-species-detected-by-fish
#4
Yan Zhao, Jihong Xie, Quanwen Dou, Junjie Wang, Zhong Zhang
The genomes of five Agropyron Gaertner, 1770 species were characterized using all potential di- or trinucleotide simple sequence repeat (SSR) motifs and four satellite DNA repeats as fluorescence in situ hybridization (FISH) probes. The sites of 5S and 45S rDNA were relatively conserved among the diploid and tetraploid species. A number of sites for the dinucleotide SSRs AC, AG, and pSc119.2 was detected in all investigated species except A. mongolicum Keng, 1938. Several different trinucleotide SSRs were identified in different tetraploid species...
2017: Comparative Cytogenetics
https://www.readbyqxmd.com/read/29068370/rna-seq-analysis-of-plant-maturity-in-crested-wheatgrass-agropyron-cristatum-l
#5
Fangqin Zeng, Bill Biligetu, Bruce Coulman, Michael P Schellenberg, Yong-Bi Fu
Crested wheatgrass (Agropyron cristatum L.) breeding programs aim to develop later maturing cultivars for extending early spring grazing in Western Canada. Plant maturity is a complex genetic trait, and little is known about genes associated with late maturity in this species. An attempt was made using RNA-Seq to profile the transcriptome of crested wheatgrass maturity and to analyze differentially expressed genes (DEGs) between early and late maturing lines. Three cDNA libraries for each line were generated by sampling leaves at the stem elongation stage, spikes at the boot and anthesis stages...
October 25, 2017: Genes
https://www.readbyqxmd.com/read/29030254/complete-structure-and-variation-of-the-chloroplast-genome-of-agropyron-cristatum-l-gaertn
#6
Ning Chen, Li-Na Sha, Zhen-Zhen Dong, Chao Tang, Yi Wang, Hou-Yang Kang, Hai-Qin Zhang, Xue-Bin Yan, Yong-Hong Zhou, Xing Fan
Agropyron cristatum (L.) Gaertner, a perennial grass in the tribe Triticeae (Poaceae), is a wild relative of cereal crops that is suitable for genetic improvement. In this study, we first sequenced the complete chloroplast (cp) genome of Ag. cristatum using Hiseq4000 PE150. The Ag. cristatum chloroplast genome is 135,554bp in length, has a typical quadripartite structure and contains 76 protein-coding genes, 29 tRNA genes and four rRNA genes. The cp genome of Ag. cristatum was used for comparison with other seven Triticeae species...
January 15, 2018: Gene
https://www.readbyqxmd.com/read/28983310/rna-seq-analysis-provides-the-first-insights-into-the-phylogenetic-relationship-and-interspecific-variation-between-agropyron-cristatum-and-wheat
#7
Shenghui Zhou, Baiqiang Yan, Fei Li, Jinpeng Zhang, Jing Zhang, Huihui Ma, Weihua Liu, Yuqing Lu, Xinming Yang, Xiuquan Li, Xu Liu, Lihui Li
Agropyron cristatum, which is a wild grass of the tribe Triticeae, grows widely in harsh environments and provides many desirable genetic resources for wheat improvement. However, unclear interspecific phylogeny and genome-wide variation has limited the utilization of A. cristatum in the production of superior wheat varieties. In this study, by sequencing the transcriptome of the representative tetraploid A. cristatum Z559 and the common wheat variety Fukuhokomugi (Fukuho), which are often used as parents in a wide cross, their phylogenetic relationship and interspecific variation were dissected...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28931929/a-resource-of-large-scale-molecular-markers-for-monitoring-agropyron-cristatum-chromatin-introgression-in-wheat-background-based-on-transcriptome-sequences
#8
Jinpeng Zhang, Weihua Liu, Yuqing Lu, Qunxing Liu, Xinming Yang, Xiuquan Li, Lihui Li
Agropyron cristatum is a wild grass of the tribe Triticeae and serves as a gene donor for wheat improvement. However, very few markers can be used to monitor A. cristatum chromatin introgressions in wheat. Here, we reported a resource of large-scale molecular markers for tracking alien introgressions in wheat based on transcriptome sequences. By aligning A. cristatum unigenes with the Chinese Spring reference genome sequences, we designed 9602 A. cristatum expressed sequence tag-sequence-tagged site (EST-STS) markers for PCR amplification and experimental screening...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28921769/construction-of-agropyron-gaertn-genetic-linkage-maps-using-a-wheat-660k-snp-array-reveals-a-homoeologous-relationship-with-the-wheat-genome
#9
Shenghui Zhou, Jinpeng Zhang, Yonghe Che, Weihua Liu, Yuqing Lu, Xinming Yang, Xiuquan Li, Jizeng Jia, Xu Liu, Lihui Li
Agropyron Gaertn. (P genome) is a wild relative of wheat that harbours many genetic variations that could be used to increase the genetic diversity of wheat. To agronomically transfer important genes from the P genome to a wheat chromosome by induced homoeologous pairing and recombination, it is necessary to determine the chromosomal relationships between Agropyron and wheat. Here, we report using the wheat 660K single nucleotide polymorphism (SNP) array to genotype a segregating Agropyron F1 population derived from an interspecific cross between two cross-pollinated diploid collections 'Z1842' [A...
September 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28886152/molecular-cytogenetic-identification-of-a-novel-wheat-agropyron-elongatum-chromosome-translocation-line-with-powdery-mildew-resistance
#10
Xiaojun Li, Xiaoling Jiang, Xiangdong Chen, Jie Song, Cuicui Ren, Yajuan Xiao, Xiaohui Gao, Zhengang Ru
Agropyron elongatum (Host.) Neviski (synonym, Thinopyrum ponticum Podp., 2n = 70) has been used extensively as a valuable source for wheat breeding. Numerous chromosome fragments containing valuable genes have been successfully translocated into wheat from A. elongatum. However, reports on the transfer of powdery mildew resistance from A. elongatum to wheat are rare. In this study, a novel wheat-A. elongatum translocation line, 11-20-1, developed and selected from the progenies of a sequential cross between wheat varieties (Lankaoaizaoba, Keyu 818 and BainongAK 58) and A...
2017: PloS One
https://www.readbyqxmd.com/read/28797236/genome-wide-transcriptome-profiling-provides-overwintering-mechanism-of-agropyron-mongolicum
#11
Jiancai Du, Xiaoquan Li, Tingting Li, Dongyang Yu, Bing Han
BACKGROUND: The mechanism of winter survival for perennials involves multiple levels of gene regulation, especially cold resistance. Agropyron mongolicum is one important perennial grass species, but there is little information regarding its overwintering mechanism. We performed a comprehensive transcriptomics study to evaluate global gene expression profiles regarding the winter survival of Agropyron mongolicum. A genome-wide gene expression analysis involving four different periods was identified...
August 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28533082/contrasting-evolutionary-patterns-of-multiple-loci-uncover-new-aspects-in-the-genome-origin-and-evolutionary-history-of-leymus-triticeae-poaceae
#12
Li-Na Sha, Xing Fan, Jun Li, Jin-Qiu Liao, Jian Zeng, Yi Wang, Hou-Yang Kang, Hai-Qin Zhang, You-Liang Zheng, Yong-Hong Zhou
Leymus Hochst. (Triticeae: Poaceae), a group of allopolyploid species with the NsXm genomes, is a perennial genus with diversity in morphology, cytology, ecology, and distribution in the Triticeae. To investigate the genome origin and evolutionary history of Leymus, three unlinked low-copy nuclear genes (Acc1, Pgk1, and GBSSI) and three chloroplast regions (trnL-F, matK, and rbcL) of 32 Leymus species were analyzed with those of 36 diploid species representing 18 basic genomes in the Triticeae. The phylogenetic relationships were reconstructed using Bayesian inference, Maximum parsimony, and NeighborNet methods...
May 19, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28531235/rna-seq-analysis-of-gene-expression-for-floral-development-in-crested-wheatgrass-agropyron-cristatum-l
#13
Fangqin Zeng, Bill Biligetu, Bruce Coulman, Michael P Schellenberg, Yong-Bi Fu
Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is widely used for early spring grazing in western Canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. However, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant maturity in this species. In this study, RNA-Seq and differential gene expression analysis were performed to investigate gene expression for floral initiation and development in crested wheatgrass...
2017: PloS One
https://www.readbyqxmd.com/read/28394901/chromosomal-localization-of-genes-conferring-desirable-agronomic-traits-from-agropyron-cristatum-chromosome-1p
#14
Cuili Pan, Qingfeng Li, Yuqing Lu, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable genes for wheat breeding. To transfer these genes into wheat, a series of wheat-A. cristatum derivatives have been obtained in our laboratory. In this study, a wheat-A. cristatum derivative II-3-1 was obtained, which was proven to contain a 1P (1A) disomic substitution and 2P disomic addition line with 40 wheat chromosomes and two pairs of A. cristatum chromosomes by genomic in situ hybridization (GISH) and molecular markers analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28337069/detection-of-alien-genetic-introgressions-in-bread-wheat-using-dot-blot-genomic-hybridisation
#15
María-Dolores Rey, Pilar Prieto
Simple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilense addition or substitution lines in wheat when molecular markers are difficult to use. Based on genomic in situ hybridisation, DNA samples from wheat lines carrying putatively H. chilense introgressions were immobilised on a membrane, blocked with wheat genomic DNA and hybridised with biotin-labelled H...
2017: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/28278169/molecular-cytogenetic-fish-and-genome-analysis-of-diploid-wheatgrasses-and-their-phylogenetic-relationship
#16
Gabriella Linc, Eszter Gaál, István Molnár, Diana Icsó, Ekaterina Badaeva, Márta Molnár-Láng
This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes...
2017: PloS One
https://www.readbyqxmd.com/read/28171833/assessing-chilling-and-drought-tolerance-of-different-plant-genera-on-extensive-green-roofs-in-an-arid-climate-region-in-iran
#17
Navid Vahdati, Ali Tehranifar, Fatemeh Kazemi
The harsh and stressful growing environment of extensive green roofs especially in arid environments allows a limited range of plant species to survive. Therefore, achieving plantings to survive in such conditions is a significant challenge. This paper describes an experiment investigating plant selections for extensive green roofs based on chilling (cold season) and drought (warm season) conditions of Iran. Nine species were selected from the three major taxonomic and functional plant groups that are commonly used on extensive green roofs including grasses, groundcovers and sedums...
May 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28040531/traditionally-used-medicinal-plants-against-uncomplicated-urinary-tract-infections-hexadecyl-coumaric-acid-ester-from-the-rhizomes-of-agropyron-repens-l-p-beauv-with-antiadhesive-activity-against-uropathogenic-e-coli
#18
Shabnam Sarshar Beydokthi, Jandirk Sendker, Simone Brandt, Andreas Hensel
The rhizomes from Agropyron repens are traditionally used for the treatment of uncomplicated urinary tract infections. Extracts prepared with solvents of different polarity did not show any cytotoxic effects against different strains of uropathogenic E. coli (UPEC) and human T24 bladder cells under in vitro conditions. Significant antiadhesive activity against the bacterial attachment to human T24 bladder cells was found for an acetone extract (AAE) at concentrations >250μg/mL. More hydrophilic extracts did not influence the bacterial attachment to the eukaryotic host cells...
March 2017: Fitoterapia
https://www.readbyqxmd.com/read/27832372/isolation-and-application-of-p-genome-specific-dna-sequences-of-agropyron-gaertn-in-triticeae
#19
Haiming Han, Weihua Liu, Yuqing Lu, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Zanmin Hu, Lihui Li
Different types of P genome sequences and markers were developed, which could be used to analyze the evolution of P genome in Triticeae and identify precisely wheat- A. cristatum introgression lines. P genome of Agropyron Gaertn. plays an important role in Triticeae and could provide many desirable genes conferring high yield, disease resistance, and stress tolerance for wheat genetic improvement. Therefore, it is significant to develop specific sequences and functional markers of P genome. In this study, 126 sequences were isolated from the degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) products of microdissected chromosome 6PS...
February 2017: Planta
https://www.readbyqxmd.com/read/27824906/chromosomal-localization-of-genes-conferring-desirable-agronomic-traits-from-wheat-agropyron-cristatum-disomic-addition-line-5113
#20
Qingfeng Li, Yuqing Lu, Cuili Pan, Miaomiao Yao, Jinpeng Zhang, Xinming Yang, Weihua Liu, Xiuquan Li, Yajun Xi, Lihui Li
Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust...
2016: PloS One
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