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https://www.readbyqxmd.com/read/28394901/chromosomal-localization-of-genes-conferring-desirable-agronomic-traits-from-agropyron-cristatum-chromosome-1p
#1
Cuili Pan, Qingfeng Li, Yuqing Lu, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Lihui Li, Weihua Liu
Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable genes for wheat breeding. To transfer these genes into wheat, a series of wheat-A. cristatum derivatives have been obtained in our laboratory. In this study, a wheat-A. cristatum derivative II-3-1 was obtained, which was proven to contain a 1P (1A) disomic substitution and 2P disomic addition line with 40 wheat chromosomes and two pairs of A. cristatum chromosomes by genomic in situ hybridization (GISH) and molecular markers analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28337069/detection-of-alien-genetic-introgressions-in-bread-wheat-using-dot-blot-genomic-hybridisation
#2
María-Dolores Rey, Pilar Prieto
Simple, reliable methods for the identification of alien genetic introgressions are required in plant breeding programmes. The use of genomic dot-blot hybridisation allows the detection of small Hordeum chilense genomic introgressions in the descendants of genetic crosses between wheat and H. chilense addition or substitution lines in wheat when molecular markers are difficult to use. Based on genomic in situ hybridisation, DNA samples from wheat lines carrying putatively H. chilense introgressions were immobilised on a membrane, blocked with wheat genomic DNA and hybridised with biotin-labelled H...
2017: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/28278169/molecular-cytogenetic-fish-and-genome-analysis-of-diploid-wheatgrasses-and-their-phylogenetic-relationship
#3
Gabriella Linc, Eszter Gaál, István Molnár, Diana Icsó, Ekaterina Badaeva, Márta Molnár-Láng
This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes...
2017: PloS One
https://www.readbyqxmd.com/read/28171833/assessing-chilling-and-drought-tolerance-of-different-plant-genera-on-extensive-green-roofs-in-an-arid-climate-region-in-iran
#4
Navid Vahdati, Ali Tehranifar, Fatemeh Kazemi
The harsh and stressful growing environment of extensive green roofs especially in arid environments allows a limited range of plant species to survive. Therefore, achieving plantings to survive in such conditions is a significant challenge. This paper describes an experiment investigating plant selections for extensive green roofs based on chilling (cold season) and drought (warm season) conditions of Iran. Nine species were selected from the three major taxonomic and functional plant groups that are commonly used on extensive green roofs including grasses, groundcovers and sedums...
February 4, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28040531/traditionally-used-medicinal-plants-against-uncomplicated-urinary-tract-infections-hexadecyl-coumaric-acid-ester-from-the-rhizomes-of-agropyron-repens-l-p-beauv-with-antiadhesive-activity-against-uropathogenic-e-coli
#5
Shabnam Sarshar Beydokthi, Jandirk Sendker, Simone Brandt, Andreas Hensel
The rhizomes from Agropyron repens are traditionally used for the treatment of uncomplicated urinary tract infections. Extracts prepared with solvents of different polarity did not show any cytotoxic effects against different strains of uropathogenic E. coli (UPEC) and human T24 bladder cells under in vitro conditions. Significant antiadhesive activity against the bacterial attachment to human T24 bladder cells was found for an acetone extract (AAE) at concentrations >250μg/mL. More hydrophilic extracts did not influence the bacterial attachment to the eukaryotic host cells...
March 2017: Fitoterapia
https://www.readbyqxmd.com/read/27832372/isolation-and-application-of-p-genome-specific-dna-sequences-of-agropyron-gaertn-in-triticeae
#6
Haiming Han, Weihua Liu, Yuqing Lu, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Zanmin Hu, Lihui Li
Different types of P genome sequences and markers were developed, which could be used to analyze the evolution of P genome in Triticeae and identify precisely wheat- A. cristatum introgression lines. P genome of Agropyron Gaertn. plays an important role in Triticeae and could provide many desirable genes conferring high yield, disease resistance, and stress tolerance for wheat genetic improvement. Therefore, it is significant to develop specific sequences and functional markers of P genome. In this study, 126 sequences were isolated from the degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) products of microdissected chromosome 6PS...
February 2017: Planta
https://www.readbyqxmd.com/read/27824906/chromosomal-localization-of-genes-conferring-desirable-agronomic-traits-from-wheat-agropyron-cristatum-disomic-addition-line-5113
#7
Qingfeng Li, Yuqing Lu, Cuili Pan, Miaomiao Yao, Jinpeng Zhang, Xinming Yang, Weihua Liu, Xiuquan Li, Yajun Xi, Lihui Li
Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust...
2016: PloS One
https://www.readbyqxmd.com/read/27771744/mapping-of-novel-powdery-mildew-resistance-gene-s-from-agropyron-cristatum-chromosome-2p
#8
Huanhuan Li, Bo Jiang, Jingchang Wang, Yuqing Lu, Jinpeng Zhang, Cuili Pan, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
A physical map of Agropyron cristatum 2P chromosome was constructed for the first time and the novel powdery mildew resistance gene(s) from chromosome 2P was(were) also mapped. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies showed that wheat-A. cristatum 2P disomic addition line II-9-3 displayed high resistance to powdery mildew, and the resistance was attributable to A. cristatum chromosome 2P. To utilize and physically map the powdery mildew resistance gene(s), 15 wheat-A...
January 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27643577/cytological-and-molecular-analysis-of-wheat-agropyron-cristatum-translocation-lines-with-6p-chromosome-fragments-conferring-superior-agronomic-traits-in-common-wheat
#9
Liqiang Song, Yuqing Lu, Jinpeng Zhang, Cuili Pan, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat - A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat - A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27551349/simultaneous-visualization-of-different-genomes-j-jst-and-st-in-a-thinopyrum-intermedium-%C3%A3-thinopyrum-ponticum-synthetic-hybrid-poaceae-and-in-its-parental-species-by-multicolour-genomic-in-situ-hybridization-mcgish
#10
Klaudia Kruppa, Márta Molnár-Láng
Multicolour genomic in situ hybridization (mcGISH) using total genomic DNA probes from Thinopyrum bessarabicum (Săvulescu & Rayss, 1923) Á. Löve, 1984 (genome J(b) or E(b), 2n = 14), and Pseudoroegneria spicata (Pursh, 1814) Á. Löve, 1980 (genome St, 2n = 14) was used to characterize the mitotic metaphase chromosomes of a synthetic hybrid of Thinopyrum intermedium (Host, 1805) Barkworth & D.R. Dewey, 1985 and Thinopyrum ponticum (Podpěra, 1902) Z.-W. Liu et R.-C.Wang, 1993 named "Agropyron glael" and produced by N...
2016: Comparative Cytogenetics
https://www.readbyqxmd.com/read/27459347/transferring-desirable-genes-from-agropyron-cristatum-7p-chromosome-into-common-wheat
#11
Mingjie Lu, Yuqing Lu, Huanhuan Li, Cuili Pan, Yong Guo, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Wheat-Agropyron cristatum 7P disomic addition line Ⅱ-5-1, derived from the distant hybridization between A. cristatum (2n = 4x = 28, PPPP) and the common wheat cv. Fukuhokomugi (Fukuho), displays numerous desirable agronomic traits, including enhanced thousand-grain weight, smaller flag leaf, and enhanced tolerance to drought. In order to transfer these traits into common wheat, Ⅱ-5-1 was induced by 60Co-γ ray, leading to the creation of 18 translocation lines and three deletion lines. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) indicated that multiple wheat chromosomes were involved in the translocation events, including chromosome 2A, 3A, 5A, 7A, 3B, 5B, 7B, 3D and 7D...
2016: PloS One
https://www.readbyqxmd.com/read/27246315/an-intercalary-translocation-from-agropyron-cristatum-6p-chromosome-into-common-wheat-confers-enhanced-kernel-number-per-spike
#12
Jing Zhang, Jinpeng Zhang, Weihua Liu, Xiaoyang Wu, Xinming Yang, Xiuquan Li, Yuqing Lu, Lihui Li
This study explored 6P chromosomal translocations in wheat, and determined the effects of 6P intercalary chromosome segments on kernel number per wheat spike. Exploiting and utilising gene(s) from wild relative species has become an essential strategy for wheat crop improvement. In the translocation line Pubing2978, the intercalary 6P chromosome segment from Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) carried valuable multi-kernel gene(s) and was selected from the offspring of the common wheat plant Fukuho and the irradiated wheat-A...
October 2016: Planta
https://www.readbyqxmd.com/read/27215032/-genealogical-analysis-of-the-use-of-two-wheatgrass-agropyron-species-in-common-wheat-triticum-aestivum-l-breeding-for-disease-resistance
#13
S P Martynova, T V Dobrotvorskaya, V A Krupnov
During the last 80 years, in order to increase the genetic variability of wheat, translocations containing nine elongated wheatgrass (Agropyron elongatum) and eight intermediate wheatgrass (Agropyron intermedium) genes, which control resistance to pathogens, were transferred to this crop culture. Genealogical and statistical analysis of 1500 varieties developed using the wheatgrass gave evidence of the continuing increase in the proportion of such varieties in the total number of wheat varieties over the last half-century...
February 2016: Genetika
https://www.readbyqxmd.com/read/27179541/seed-germination-of-seven-desert-plants-and-implications-for-vegetation-restoration
#14
Liming Lai, Lijun Chen, Lianhe Jiang, Jihua Zhou, Yuanrun Zheng, Hideyuki Shimizu
Germination cues reflect the conditions under which a species is likely to succeed in recruitment. Therefore, knowledge of the seed germination characteristics of key plant species in desertified areas is essential for restoration. The aims of this study were to evaluate the seed germination responses of seven native species, and to explore the implications for vegetation restoration. Seeds of seven desert species were sown in Petri dishes and subjected to various temperature and light conditions. The seeds germinated well at day/night temperatures of 25/15 °C and 30/20 °C but poorly at 35/25 °C...
2016: AoB Plants
https://www.readbyqxmd.com/read/27125388/genetic-analysis-of-a-novel-broad-spectrum-powdery-mildew-resistance-gene-from-the-wheat-agropyron-cristatum-introgression%C3%A2-line-pubing-74
#15
Yuqing Lu, Miaomiao Yao, Jinpeng Zhang, Liqiang Song, Weihua Liu, Xinming Yang, Xiuquan Li, Lihui Li
A novel broad-spectrum powdery mildew resistance gene PmPB74 was identified in wheat- Agropyron cristatum introgression line Pubing 74. Development of wheat cultivars with broad-spectrum, durable resistance to powdery mildew has been restricted by lack of superior genetic resources. In this study, a wheat-A. cristatum introgression line Pubing 74, originally selected from a wide cross between the common wheat cultivar Fukuhokomugi (Fukuho) and Agropyron cristatum (L.) Gaertn (2n = 4x = 28; genome PPPP), displayed resistance to powdery mildew at both the seedling and adult stages...
September 2016: Planta
https://www.readbyqxmd.com/read/26922334/mcgish-identification-and-phenotypic-description-of-leaf-rust-and-yellow-rust-resistant-partial-amphiploids-originating-from-a-wheat%C3%A2-%C3%A3-%C3%A2-thinopyrum-synthetic-hybrid-cross
#16
Klaudia Kruppa, Edina Türkösi, Marianna Mayer, Viola Tóth, Gyula Vida, Éva Szakács, Márta Molnár-Láng
A Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid wheatgrass is an excellent source of leaf and stem rust resistance produced by N.V.Tsitsin. Wheat line Mv9kr1 was crossed with this hybrid (Agropyron glael) in Hungary in order to transfer its advantageous agronomic traits into wheat. As the wheat parent was susceptible to leaf rust, the transfer of resistance was easily recognizable in the progenies. Three different partial amphiploid lines with leaf rust resistance were selected from the wheat/Thinopyrum hybrid derivatives by multicolour genomic in situ hybridization...
November 2016: Journal of Applied Genetics
https://www.readbyqxmd.com/read/26920547/physical-mapping-of-agropyron-cristatum-chromosome-6p-using-deletion-lines-in-common-wheat-background
#17
Liqiang Song, Yuqing Lu, Jinpeng Zhang, Cuili Pan, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Genetically stable deletion lines of Agropyron cristatum chromosome 6P in common wheat background were generated, which allowed for physical mapping of 255 6P-specific STS markers and leaf rust resistance gene(s). Chromosomal deletion lines are valuable tools for gene discovery and localization. The chromosome 6P of Agropyron cristatum (2n = 4x = 28, PPPP) confers many desirable agronomic traits to common wheat, such as higher grain number per spike, multiple fertile tiller number, and enhanced resistance to certain diseases...
May 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/26909915/cloning-and-expression-analysis-of-cysteine-protease-gene-mwcp-in-agropyron-mongolicum-keng
#18
T G B Y Ao, M L Lang, Y Q Li, Y Zhao, L C Wang, X J Yang
In this study, a cysteine protease gene (MwCP) from Agropyron mongolicum Keng was isolated using RACE. Sequence analysis indicated that MwCP was 1473 bp, and it contained a 1134-bp open reading frame, which encoded 377 amino acids with a 24-amino acid N-terminal signal peptide. The results indicated that the MwCP protein was a new member of the papain C1A family, and it was predicted to be an extracellular, secretory stable hydrophilic protein. The secondary structure of MwCP was mainly composed of α-helices and random coils, and the space structure primarily contained α-helices, β-sheets, and β-turns...
January 22, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/26731742/production-and-identification-of-wheat-agropyron-cristatum-2p-translocation-lines
#19
Huanhuan Li, Mingjie Lv, Liqiang Song, Jinpeng Zhang, Ainong Gao, Lihui Li, Weihua Liu
Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable traits that can be transferred to common wheat through breeding programs. The wheat-A. cristatum disomic addition and translocation lines can be used as bridge materials to introduce alien chromosomal segments to wheat. Wheat-A. cristatum 2P disomic addition line II-9-3 was highly resistant to powdery mildew and leaf rust, which was reported in our previous study. However, some translocation lines induced from II-9-3 have not been reported...
2016: PloS One
https://www.readbyqxmd.com/read/26634575/-antiurolithiasic-effect-of-a-plant-mixture-of-herniaria-glabra-agropyron-repens-equisetum-arvense-and-sambucus-nigra-herbensurina%C3%A2-in-the-prevention-of-experimentally-induced-nephrolithiasis-in-rats
#20
Anna Crescenti, Francesc Puiggròs, Arnau Colomé, Josep Antón Poch, Antoni Caimari, Josep Maria Bas, Noemí Boqué, Lluís Arola
OBJECTIVE: To determine the effect of a botanical formulation of Herniaria glabra, Agropyron repens, Equisetum arvense, and Sambucus nigra as a preventive agent in an experimentally induced nefrolithiasis model in rats. METHODS: Six groups of six Wistar male rats each were induced for nefrolithiasis by treatment with 0.75% ethylene glycol (EG) and 1% ammonium chloride for three days and then EG only for 15 days. One group was treated with placebo (control group) and the other groups (treated groups) were treated with 30 mg/Kg, 60 mg/Kg, 125 mg/Kg, 250 mg/Kg and 500 mg/Kg of the plant extract formulation (PEF)...
December 2015: Archivos Españoles de Urología
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