keyword
MENU ▼
Read by QxMD icon Read
search

vlp analysis

keyword
https://www.readbyqxmd.com/read/28921433/large-scale-transient-transfection-of-suspension-mammalian-cells-for-vlp-production
#1
Laura Cervera, Amine A Kamen
Large-scale transient transfection of mammalian cell suspension cultures enables the production of biological products in sufficient quantity and under stringent quality attributes to perform accelerated in vitro evaluations and has the potential to support preclinical or even clinical studies. Here we describe the methodology to produce VLPs in a 3L bioreactor, using suspension HEK 293 cells and PEIPro as a transfection reagent. Cells are grown in the bioreactor to 1 × 10(6) cells/mL and transfected with a plasmid DNA-PEI complex at a ratio of 1:2...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28893480/production-of-highly-immunogenic-virus-like-particles-of-bovine-papillomavirus-type-6-in-silkworm-pupae
#2
Satoko Watanabe, Tetsuya Iizuka, Shinichi Hatama, Toru Kanno, Masaji Mase, Tomoyuki Shibahara
Bovine papillomaviruses (BPVs) are the causative agent of bovine teat papillomatosis, which can lead to severe economic losses in dairy cattle. Among the 14 identified BPV genotypes, BPV type 6 (BPV6) is the most frequently detected in teat papilloma lesions, and is therefore thought to play a major role in teat papillomatosis. To develop an effective vaccine against BPV6 infection, we produced virus-like particles of BPV6 (BPV6-VLP) in silkworm (Bombyx mori) pupae and purified these by heparin affinity chromatography using a single column...
September 8, 2017: Vaccine
https://www.readbyqxmd.com/read/28876928/md-simulations-of-viruslike-particles-with-supra-cg-solvation-affordable-to-desktop-computers
#3
Matı As R Machado, Humberto C González, Sergio Pantano
Viruses are tremendously efficient molecular devices that optimize the packing of genetic material using a minimalistic number of proteins to form a capsid or envelope that protects them from external threats, being also part of cell recognition, fusion, and budding machineries. Progress in experimental techniques has provided a large number of high-resolution structures of viruses and viruslike particles (VLP), while molecular dynamics simulations may furnish lively and complementary insights on the fundamental forces ruling viral assembly, stability, and dynamics...
September 20, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28842921/development-of-high-throughput-and-high-sensitivity-capillary-gel-electrophoresis-platform-method-for-western-eastern-and-venezuelan-equine-encephalitis-wevee-virus-like-particles-vlps-purity-determination-and-characterization
#4
Deepika Gollapudi, Diane L Wycuff, Richard M Schwartz, Jonathan W Cooper, K C Cheng
In this paper, we describe development of a high-throughput, highly-sensitive method based on Lab Chip CGE-SDS platform for purity determination and characterization of virus-like particle (VLP) vaccines. A capillary gel electrophoresis approach requiring about 41 seconds per sample for analysis and demonstrating sensitivity to protein initial concentrations as low as 20 μg/mL, this method has been used previously to evaluate monoclonal antibodies, but this application for lot release assay of VLPs using this platform is unique...
August 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28841998/quantitation-and-biospecific-identification-of-virus-like-particles-of-human-papillomavirus-by-capillary-electrophoresis
#5
Virginie Bettonville, Jérôme T J Nicol, Tania Furst, Nicolas Thelen, Géraldine Piel, Marc Thiry, Marianne Fillet, Nathalie Jacobs, Anne-Catherine Servais
Capillary electrophoresis (CE) for HPV-VLP quantitation is a very interesting alternative technique compared to those currently used in viral analysis, such as SDS-PAGE, Western blot or protein assay that are destructive and semi-quantitative or non specific. In this study, the quantitative performance of the CE method was evaluated. A main issue in virus quantitation is the absence of reference material. Therefore, the concentration of a HPV16-VLP sample produced in the laboratory was determined using ELISA with Gardasil(®), after adjuvant dissolution, as reference material and conformational H16...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28754559/enzymatic-cleavage-promotes-disassembly-of-gii-3-norovirus-virus-like-particles-and-its-binding-to-salivary-histo-blood-group-antigens
#6
Yuqi Huo, Wenhui Wang, Lijun Zheng, Xuhui Chen, Shuo Shen, Mingchen Wang
In this study, we found that addition of fecal extract significantly promoted the binding of GII.3 NoV VLPs to salivary HBGAs. SDS-PAGE analysis indicated that major capsid proteins (VP1) were cleaved into two major bands with molecular weights of 26 and 31kD, respectively. Pretreatment of fecal extract by boiling or addition of protease inhibitor cocktail or type specific protease inhibitor (leupeptin and aprotinin) during incubation all decreased VP1 cleavage and its binding to salivary HBGAs. Trypsin digestion led to cleavage of VP1 and promoted its binding to HBGAs, suggesting that the active enzyme(s) might be trypsin or trypsin-like enzymes...
July 25, 2017: Virus Research
https://www.readbyqxmd.com/read/28753950/perturbation-of-human-t-cell-leukemia-virus-type-1-particle-morphology-by-differential-gag-co-packaging
#7
José O Maldonado, Isaac Angert, Sheng Cao, Serkan Berk, Wei Zhang, Joachim D Mueller, Louis M Mansky
Human T-cell leukemia virus type 1 (HTLV-1) is an important cancer-causing human retrovirus that has infected approximately 15 million individuals worldwide. Many aspects of HTLV-1 replication, including virus particle structure and assembly, are poorly understood. Group-specific antigen (Gag) proteins labeled at the carboxy terminus with a fluorophore protein have been used extensively as a surrogate for fluorescence studies of retroviral assembly. How these tags affect Gag stoichiometry and particle morphology has not been reported in detail...
July 19, 2017: Viruses
https://www.readbyqxmd.com/read/28725500/volar-plate-fixation-in-patients-older-than-70-years-with-ao-type-c-distal-radial-fractures-clinical-and-radiologic-outcomes
#8
Nicolas S Piuzzi, Ezequiel E Zaidenberg, Matias Pereira Duarte, Jorge G Boretto, Agustin Donndorff, Gerardo Gallucci, Pablo De Carli
Introduction  Treatment of unstable distal radial fractures (DRFs) in elderly patients is controversial, and considering the increasing life expectancy, their appropriate treatment is of growing importance. Our aim was to analyze the clinical and radiologic outcomes in the elderly patients with AO type C DRF treated with volar locking plate (VLP). Materials and Methods  Between 2007 and 2011, 572 DRFs were operated on in our hospital with open reduction and internal fixation with VLP. Of these, only 64 patients (66 DRFs) met the selection criteria (AO type C DRF, age > 70 years, minimum 12-month follow-up)...
August 2017: Journal of Wrist Surgery
https://www.readbyqxmd.com/read/28578190/correlation-between-dynamic-light-scattering-and-size-exclusion-high-performance-liquid-chromatography-for-monitoring-the-effect-of-ph-on-stability-of-biopharmaceuticals
#9
Medhat A Al-Ghobashy, Moushira M Mostafa, Heba S Abed, Faten A Fathalla, Maissa Y Salem
Aggregate formation is a major problem affecting both safety and efficacy of biopharmaceuticals and is associated with protein immunogenicity. Size exclusion high performance liquid chromatography (SE-HPLC) has always been the gold standard technique for detection and determination of protein aggregates. However, large protein aggregates may be filtered off and build up on top of the column leading to deterioration in column performance. Moreover, low-affinity protein aggregates may dissociate during analysis and thus not detected...
August 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28573614/modeling-ebolavirus-budding-with-virus-like-particles
#10
Olivier Reynard, Mathieu Mateo
About 15 years ago, several groups initially described the release of virus like particles (VLPs) upon expression of Ebola virus VP40 in mammalian cells. Further development of the protocol later allowed for the dissection of the Ebola virus budding mechanism and for the identification of critical VP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even been proposed as vaccine candidates against Ebola virus disease...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28542275/combination-of-the-immunization-with-the-sequence-close-to-the-consensus-sequence-and-two-dna-prime-plus-one-vlp-boost-generate-h5-hemagglutinin-specific-broad-neutralizing-antibodies
#11
COMPARATIVE STUDY
Guiqin Wang, Renfu Yin, Paul Zhou, Zhuang Ding
Hemagglutinin (HA) head has long been considered to be able to elicit only a narrow, strain-specific antibody response as it undergoes rapid antigenic drift. However, we previously showed that a heterologous prime-boost strategy, in which mice were primed twice with DNA encoding HA and boosted once with virus-like particles (VLP) from an H5N1 strain A/Thailand/1(KAN)-1/2004 (noted as TH DDV), induced anti-head broad cross-H5 neutralizing antibody response. To explain why TH DDV immunization could generate such breadth, we systemically compared the neutralization breadth and potency between TH DDV sera and immune sera elicited by TH DDD (three times of DNA immunizations), TH VVV (three times of VLP immunizations), TH DV (one DNA prime plus one VLP boost) and TK DDV (plasmid DNA and VLP derived from another H5N1 strain, A/Turkey/65596/2006)...
2017: PloS One
https://www.readbyqxmd.com/read/28540352/dataset-on-galanin-receptor-3-mutants-that-improve-recombinant-receptor-expression-and-stability-in-an-agonist-and-antagonist-bound-form
#12
Thao T Ho, Jasmine T Nguyen, Juping Liu, Pawel Stanczak, Aaron A Thompson, Yingzhuo G Yan, Jasmine Chen, Charles K Allerston, Charles L Dillard, Hao Xu, Nicholas J Shoger, Jill S Cameron, Mark E Massari, Kathleen Aertgeerts
Galanin Receptor 3 (GALR3) is a G-protein-coupled receptor with a widespread distribution in the brain and plays a role in a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. Therefore, GALR3 is considered an attractive CNS drug target (Freimann et al., 2015) [1]. This dataset contains GALR3 point mutants that improve recombinant protein expression and thermal stability of the receptor contained in virus-like particles (VLPs) or obtained by detergent-purification of baculovirus-infected insect cells...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28537193/isolated-potato-virus-a-coat-protein-possesses-unusual-properties-and-forms-different-short-virus-like-particles
#13
Alexander L Ksenofontov, Eugeny N Dobrov, Natalia V Fedorova, Marina V Serebryakova, Andrei N Prusov, Ludmila A Baratova, Viiu Paalme, Lilian Järvekülg, Eleonora V Shtykova
In our previous study, we have observed that the isolated coat proteins (CP) of the Potyvirus Potato Virus A (PVA) virions exhibit an intrinsic tendency to self-associate into various multimeric forms containing some fractions of cross-β-structure. In this report, we studied the effect of solution conditions on the structure and dissociation of isolated PVA CP using a number of complementary physicochemical methods. Analysis of the structure of PVA CP in solution was performed by limited proteolysis with MALDI-TOF mass spectrometry analysis, transmission electron microscopy, intrinsic fluorescence spectroscopy, and synchrotron small angle X-ray scattering (SAXS)...
June 8, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28500458/occurrence-of-vascular-lake-phenomenon-as-a-predictor-of-improved-tumor-response-in-hcc-patients-that-underwent-deb-tace
#14
Rafael Noronha Cavalcante, Felipe Nasser, Joaquim M Motta-Leal-Filho, Breno B Affonso, Francisco L Galastri, Bruna De Fina, Rodrigo G Garcia, Nelson Wolosker
PURPOSE: To evaluate incidence and predictive factors for the vascular lake phenomenon (VLP), as well as to compare local and overall tumor response in patients with and without VLP induced during DEB-TACE for HCC. METHODS: A total of 200 consecutive patients with 323 HCC nodules underwent first-session DEB-TACE from 2011 to 2014. Patients were divided in two groups, according to the presence of the VLP during DEB-TACE. Pre- and post-treatment imaging studies (CT or MRI) were performed...
July 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28435908/development-of-a-japanese-encephalitis-virus-like-particle-vaccine-in-silkworms-using-codon-optimised-prm-and-envelope-genes
#15
Sayaka Matsuda, Reiko Nerome, Kenichi Maegawa, Akira Kotaki, Shigeo Sugita, Kazunori Kawasaki, Kazumichi Kuroda, Ryoji Yamaguchi, Tomohiko Takasaki, Kuniaki Nerome
We have successfully prepared a Japanese encephalitis virus (JEV) - Nakayama virus like particle (NVLP) vaccine using synthetic codon-optimized prM and E genes. The expression of the recombinant JEV Nakayama-BmNPV (JEV-NNPV) virus was determined in infected silkworm Bm-N cells by fluorescence and Western blot analysis. The recombinant was inoculated into silkworm pupae and the yield of Nakayama VLP (NVLP) reached a peak in the homogenates after 3 days. Additionally, in the peptide analysis of infected pupae homogenate, it appeared approximately 300-500 μg E protein/pupa were produced...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28416790/a-meta-analysis-of-cerebrospinal-fluid-visinin-like-protein-1-in-alzheimers-disease-patients-relative-to-healthy-controls-and-mild-cognitive-impairment-patients
#16
Xiaohui Hu, Yan Yang, Daokai Gong
OBJECTIVE: To compare cerebrospinal fluid visinin-like protein-1 (CSF VLP-1) in alzheimer`s disease (AD) with that in healthy controls and mild cognitive impairment (MCI) patients and find out possible sources of heterogeneity. METHODS: "Visinin-like protein-1" and "alzheimer`s disease" were employed to search "PubMed", "Springer" and "Medline" databases until July 2016 and standard mean difference (Std.MD) was calculated. Besides, subgroup analysis and meta-regression were performed to explore the possible heterogeneity sources...
April 2017: Neurosciences: the Official Journal of the Pan Arab Union of Neurological Sciences
https://www.readbyqxmd.com/read/28389838/intramedullary-nail-versus-volar-locking-plate-fixation-for-the-treatment-of-extra-articular-or-simple-intra-articular-distal-radius-fractures-systematic-review-and-meta-analysis
#17
REVIEW
Bing Zhang, Hengrui Chang, Kunlun Yu, Jiangbo Bai, Dehu Tian, Guisheng Zhang, Xinzhong Shao, Yingze Zhang
AIMS: This study aims to compare the outcomes of intramedullary nail (IMN) and volar locking plate (VLP) fixation for treatment of extra-articular or simple intra-articular distal radius fractures. METHODS: PubMed, Embase, Medline and Cochrane Collaboration Central databases were searched for studies that compared the results of IMN and VLP fixation for the treatment of distal radius fractures up to March 2016. Stata 11.0 was used to perform the meta-analysis. RESULTS: Six randomized controlled trials (RCT) and two retrospective studies were included in this review, including 463 patients...
April 7, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28357676/porcine-epidemic-diarrhea-virus-virus-like-particles-produced-in-insect-cells-induce-specific-immune-responses-in-mice
#18
Cuiling Wang, Feihu Yan, Xuexing Zheng, Hualei Wang, Hongli Jin, Chong Wang, Yongkun Zhao, Na Feng, Tiecheng Wang, Yuwei Gao, Songtao Yang, Xianzhu Xia
Porcine epidemic diarrhea virus (PEDV), which causes 80-100% mortality in neonatal piglets, is one of the most devastating viral diseases affecting swine worldwide. To date, the lack of effective vaccines and drugs is the main problem preventing control of the global spread of PEDV. In this study, we produced PEDV virus-like particles (VLPs) composed of S, M, and E proteins with a baculovirus expression system and tested them via indirect immunofluorescence assay (IFA)and Western blot analysis. Electron microscopy showed that the morphological structure of the PEDV VLPs was similar to that of the protovirus...
August 2017: Virus Genes
https://www.readbyqxmd.com/read/28350443/monitoring-the-disassembly-of-virus-like-particles-by-19-f-nmr
#19
Rogen L C Leung, Matthew D M Robinson, Alaa A A Ajabali, Gogulan Karunanithy, Brian Lyons, Ritu Raj, Saeed Raoufmoghaddam, Shabaz Mohammed, Timothy D W Claridge, Andrew J Baldwin, Benjamin G Davis
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g., nanocarriers or vaccines, but a convenient in situ technique is lacking for tracking functional status. Here, we present a simple way to monitor disassembly of (19)F-labeled VLPs derived from bacteriophage Qβ by (19)F NMR. Analysis of resonances, under a range of conditions, allowed determination not only of the particle as fully assembled but also as disassembled, as well as detection of a degraded state upon digestion by cells...
April 19, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28279830/effects-of-partially-dismantling-the-cd4-binding-site-glycan-fence-of-hiv-1-envelope-glycoprotein-trimers-on-neutralizing-antibody-induction
#20
Ema T Crooks, Keiko Osawa, Tommy Tong, Samantha L Grimley, Yang D Dai, Robert G Whalen, Daniel W Kulp, Sergey Menis, William R Schief, James M Binley
Previously, VLPs bearing JR-FL strain HIV-1 Envelope trimers elicited potent neutralizing antibodies (nAbs) in 2/8 rabbits (PLoS Pathog 11(5): e1004932) by taking advantage of a naturally absent glycan at position 197 that borders the CD4 binding site (CD4bs). In new immunizations, we attempted to improve nAb responses by removing the N362 glycan that also lines the CD4bs. All 4 rabbits developed nAbs. One targeted the N197 glycan hole like our previous sera. Two sera depended on the N463 glycan, again suggesting CD4bs overlap...
May 2017: Virology
keyword
keyword
95139
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"