Zengyuan Wang, Qian Yang, Jingyi He, Haixin Li, Xingming Pan, Zining Li, Hui-Min Xu, Jeffrey D Rudolf, Dean J Tantillo, Liao-Bin Dong
Terpene cyclization, one of the most complex chemical reactions in nature, is generally catalyzed by two classes of terpene cyclases (TCs). Cytochrome P450s that act as unexpected TC-like enzymes are known but are very rare. In this study, we genome-mined a cryptic bacterial terpenoid gene cluster, named ari, from the thermophilic actinomycete strain Amycolatopsis arida. By employing a heterologous production system, we isolated and characterized three highly oxidized eunicellane derived diterpenoids, aridacins A-C (1-3), that possess a 6/7/5-fused tricyclic scaffold...
September 21, 2023: Angewandte Chemie