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https://www.readbyqxmd.com/read/28049346/genoproteomics-assisted-improvement-of-andrographis-paniculata-toward-a-promising-molecular-and-conventional-breeding-platform-for-autogamous-plants-affecting-the-pharmaceutical-industry
#1
Alireza Valdiani, Daryush Talei, Surrinder K Lattoo, Rodomiro Ortiz, Søren Kjærsgaard Rasmussen, Jacqueline Batley, Mohd Yusop Rafii, Mahmood Maziah, Kallevettankuzhy K Sabu, Rambod Abiri, Suchirat Sakuanrungsirikul, Soon Guan Tan
Andrographis paniculata (Burm. f.) Wall. ex Nees. (AP) is a hermaphroditic, self-compatible, and habitual inbreeding plant. Its main bioactive component is andrographolide, which is capable of inducing autophagic cell death in some human cancer cells and helps fight HIV/AIDS. Increasing the andrographolide content by investigating the genetic mechanisms controlling its biosynthesis in order to improve and develop high-yielding cultivars are the main breeding targets for AP. However, there might exist some limitations or barriers for crossability within AP accessions...
January 3, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28028238/major-transitions-in-dinoflagellate-evolution-unveiled-by-phylotranscriptomics
#2
Jan Janouškovec, Gregory S Gavelis, Fabien Burki, Donna Dinh, Tsvetan R Bachvaroff, Sebastian G Gornik, Kelley J Bright, Behzad Imanian, Suzanne L Strom, Charles F Delwiche, Ross F Waller, Robert A Fensome, Brian S Leander, Forest L Rohwer, Juan F Saldarriaga
Dinoflagellates are key species in marine environments, but they remain poorly understood in part because of their large, complex genomes, unique molecular biology, and unresolved in-group relationships. We created a taxonomically representative dataset of dinoflagellate transcriptomes and used this to infer a strongly supported phylogeny to map major morphological and molecular transitions in dinoflagellate evolution. Our results show an early-branching position of Noctiluca, monophyly of thecate (plate-bearing) dinoflagellates, and paraphyly of athecate ones...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27974049/the-draft-genome-of-whitefly-bemisia-tabaci-meam1-a-global-crop-pest-provides-novel-insights-into-virus-transmission-host-adaptation-and-insecticide-resistance
#3
Wenbo Chen, Daniel K Hasegawa, Navneet Kaur, Adi Kliot, Patricia Valle Pinheiro, Junbo Luan, Marcus C Stensmyr, Yi Zheng, Wenli Liu, Honghe Sun, Yimin Xu, Yuan Luo, Angela Kruse, Xiaowei Yang, Svetlana Kontsedalov, Galina Lebedev, Tonja W Fisher, David R Nelson, Wayne B Hunter, Judith K Brown, Georg Jander, Michelle Cilia, Angela E Douglas, Murad Ghanim, Alvin M Simmons, William M Wintermantel, Kai-Shu Ling, Zhangjun Fei
BACKGROUND: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. RESULTS: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes...
December 14, 2016: BMC Biology
https://www.readbyqxmd.com/read/27941800/modulation-of-membrane-phosphoinositide-dynamics-by-the-phosphatidylinositide-4-kinase-activity-of-the-legionella-lepb-effector
#4
Na Dong, Miao Niu, Liyan Hu, Qing Yao, Rui Zhou, Feng Shao
Legionella pneumophila, the causative bacterium for Legionnaires' disease, hijacks host membrane trafficking for the maturation of the Legionella-containing vacuole (LCV). The LCV membrane mainly contains PtdIns4P, which is important for anchoring many secreted Legionella effectors onto the LCV. Here, we identify a cryptic functional domain (LepB_NTD) preceding the well-characterized RabGAP domain in the Legionella Dot/Icm type IV secretion system effector LepB. LepB_NTD alone is toxic to yeast and can disrupt the Golgi in mammalian cells...
December 12, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27866908/biosynthetic-pathway-connects-cryptic-ribosomally-synthesized-posttranslationally-modified-peptide-genes-with-pyrroloquinoline-alkaloids
#5
Peter A Jordan, Bradley S Moore
In an era where natural product biosynthetic gene clusters can be rapidly identified from sequenced genomes, it is unusual for the biosynthesis of an entire natural product class to remain unknown. Yet, the genetic determinates for pyrroloquinoline alkaloid biosynthesis have remained obscure despite their abundance and deceptive structural simplicity. In this work, we have identified the biosynthetic gene cluster for ammosamides A-C, pyrroloquinoline alkaloids from Streptomyces sp. CNR-698. Through direct cloning, heterologous expression and gene deletions we have validated the ammosamide biosynthetic gene cluster and demonstrated that these seemingly simple molecules are derived from a surprisingly complex set of biosynthetic genes that are also found in the biosynthesis of lymphostin, a structurally related pyrroloquinoline alkaloid from Salinispora and Streptomyces...
December 22, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/27759375/overexpression-of-the-global-regulator-laea-in-chaetomium-globosum-leads-to-the-biosynthesis-of-chaetoglobosin-z
#6
Tao Jiang, Menghua Wang, Li Li, Jinguang Si, Bo Song, Cao Zhou, Meng Yu, Xuewei Wang, Yonggang Zhang, Gang Ding, Zhongmei Zou
Overexpression of laeA in Chaetomium globosum CBS148.51 up-regulated expression of the chaetoglobosin gene cluster and resulted in the isolation of a new cytochalasan, chaetoglobosin Z (1), together with six known analogues, chaetoglobosins A (2), B (3), D (4), E (5), O (6), and V (7). RT-PCR analysis confirmed that the key genes in the chaetoglobosin gene cluster were significantly up-regulated. The structure of the new compound chaetoglobosin Z (1) was elucidated using NMR data. The relative and absolute configurations were determined by NOESY and electronic circular dichroism combined with quantum-chemical calculations adopting time-dependent density functional theory methods, respectively...
October 19, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/27732651/metagenomic-analysis-of-the-sponge-discodermia-reveals-the-production-of-the-cyanobacterial-natural-product-kasumigamide-by-entotheonella
#7
Yu Nakashima, Yoko Egami, Miki Kimura, Toshiyuki Wakimoto, Ikuro Abe
Sponge metagenomes are a useful platform to mine cryptic biosynthetic gene clusters responsible for production of natural products involved in the sponge-microbe association. Since numerous sponge-derived bioactive metabolites are biosynthesized by the symbiotic bacteria, this strategy may concurrently reveal sponge-symbiont produced compounds. Accordingly, a metagenomic analysis of the Japanese marine sponge Discodermia calyx has resulted in the identification of a hybrid type I polyketide synthase-nonribosomal peptide synthetase gene (kas)...
2016: PloS One
https://www.readbyqxmd.com/read/27708627/a-phenotypic-and-genotypic-analysis-of-the-antimicrobial-potential-of-cultivable-streptomyces-isolated-from-cave-moonmilk-deposits
#8
Marta Maciejewska, Delphine Adam, Loïc Martinet, Aymeric Naômé, Magdalena Całusińska, Philippe Delfosse, Monique Carnol, Hazel A Barton, Marie-Pierre Hayette, Nicolas Smargiasso, Edwin De Pauw, Marc Hanikenne, Denis Baurain, Sébastien Rigali
Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27630649/investigating-biochemical-and-developmental-dependencies-of-lignification-with-a-click-compatible-monolignol-analog-in-arabidopsis-thaliana-stems
#9
Jyotsna L Pandey, Sarah N Kiemle, Tom L Richard, Yimin Zhu, Daniel J Cosgrove, Charles T Anderson
Lignin is a key structural component of plant cell walls that provides rigidity, strength, and resistance against microbial attacks. This hydrophobic polymer also serves a crucial role in water transport. Despite its abundance and essential functions, several aspects of lignin biosynthesis and deposition remain cryptic. Lignin precursors are known to be synthesized in the cytoplasm by complex biosynthetic pathways, after which they are transported to the apoplastic space, where they are polymerized via free radical coupling reactions into polymeric lignin...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27618884/new-deferoxamine-glycoconjugates-produced-upon-overexpression-of-pathway-specific-regulatory-gene-in-the-marine-sponge-derived-streptomyces-albus-pva94-07
#10
Olga N Sekurova, Ignacio Pérez-Victoria, Jesús Martín, Kristin F Degnes, Håvard Sletta, Fernando Reyes, Sergey B Zotchev
Activation of silent biosynthetic gene clusters in Streptomyces bacteria via overexpression of cluster-specific regulatory genes is a promising strategy for the discovery of novel bioactive secondary metabolites. This approach was used in an attempt to activate a cryptic gene cluster in a marine sponge-derived Streptomyces albus PVA94-07 presumably governing the biosynthesis of peptide-based secondary metabolites. While no new peptide-based metabolites were detected in the recombinant strain, it was shown to produce at least four new analogues of deferoxamine with additional acyl and sugar moieties, for which chemical structures were fully elucidated...
August 27, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27468291/ascorbate-deficient-vtc2-mutants-in-arabidopsis-do-not-exhibit-decreased-growth
#11
Benson Lim, Nicholas Smirnoff, Christopher S Cobbett, John F Golz
In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis - VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27348741/knock-down-of-the-methyltransferase-kmt6-relieves-h3k27me3-and-results-in-induction-of-cryptic-and-otherwise-silent-secondary-metabolite-gene-clusters-in-fusarium-fujikuroi
#12
Lena Studt, Sarah M Rösler, Immo Burkhardt, Birgit Arndt, Michael Freitag, Hans-Ulrich Humpf, Jeroen S Dickschat, Bettina Tudzynski
Filamentous fungi produce a vast array of secondary metabolites (SMs) and some play a role in agriculture or pharmacology. Sequencing of the rice pathogen Fusarium fujikuroi revealed the presence of far more SM-encoding genes than known products. SM production is energy-consuming and thus tightly regulated, leaving the majority of SM gene clusters silent under laboratory conditions. One important regulatory layer in SM biosynthesis involves histone modifications that render the underlying genes either silent or poised for transcription...
November 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27311327/production-of-a-novel-amide-containing-polyene-by-activating-a-cryptic-biosynthetic-gene-cluster-in-streptomyces-sp-msc090213je08
#13
Danyao Du, Yohei Katsuyama, Hiroyasu Onaka, Manabu Fujie, Noriyuki Satoh, Kazuo Shin-Ya, Yasuo Ohnishi
Streptomyces sp. MSC090213JE08 seems to have more than 20 cryptic biosynthetic gene clusters for secondary metabolites. We aimed to activate some of them by forced production of Streptomyces antibiotic regulatory protein (SARP) family transcriptional activators. We constructed seven recombinant strains, each of which contained a SARP gene under the control of a constitutive promoter, and subjected them to comparative metabolic profiling analysis. Four of the seven strains produced nine metabolites that were hardly detected in the control strains...
August 3, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27258309/comments-on-mycobiota-and-mycotoxins-in-traditional-medicinal-seeds-from-china-toxins-2015-7-3858-3875-in-attributing-ochratoxin-a-biosynthesis-within-the-genus-penicillium-occurring-on-natural-agricultural-produce
#14
Peter Mantle, Marina Venturini Copetti, Alan Buddie, Jens Frisvad
The unusual attribution of trace amounts of ochratoxin A in some Chinese food commodities to Penicillium polonicum is questioned by European experience in searches for ochratoxinogenic food-spoilage Penicillia, where mistaken attribution is now known to have been due to cryptic Penicillium verrucosum contamination. Consequently, selection of single-spore isolates is recommended as pre-requisite for attributing mycotoxin biosynthetic potential to fungi.
2016: Toxins
https://www.readbyqxmd.com/read/27118591/deletion-of-a-yci1-domain-protein-of-candida-albicans-allows-homothallic-mating-in-mtl-heterozygous-cells
#15
Yuan Sun, Christine Gadoury, Matthew P Hirakawa, Richard J Bennett, Doreen Harcus, Anne Marcil, Malcolm Whiteway
UNLABELLED: It has been proposed that the ancestral fungus was mating competent and homothallic. However, many mating-competent fungi were initially classified as asexual because their mating capacity was hidden behind layers of regulation. For efficient in vitro mating, the essentially obligate diploid ascomycete pathogen Candida albicans has to change its mating type locus from heterozygous MTLa/α to homozygous MTLa/a or MTLα/α and then undergo an environmentally controlled epigenetic switch to the mating-competent opaque form...
April 26, 2016: MBio
https://www.readbyqxmd.com/read/27116000/multiple-oxidative-modifications-in-the-ophiobolin-biosynthesis-p450-oxidations-found-in-genome-mining
#16
Koji Narita, Ryota Chiba, Atsushi Minami, Motoichiro Kodama, Isao Fujii, Katsuya Gomi, Hideaki Oikawa
Heterologous expression of four candidate genes found in ophiobolin gene clusters from three fungal strains was employed to elucidate the late-stage biosynthetic pathway of phytotoxin ophiobolin. Expression of oblBAc (cytochrome P450) from the cryptic gene cluster gave unexpected products, and that of oblBBm/oblBEv from the gene cluster of ophiobolin producers, with oblDBm as the transporter, yielded intermediate ophiobolin C through an unusual four-step oxidation process. The observation made in this study may provide a useful guideline for the elucidation of genuine biosynthetic pathways of natural products...
May 6, 2016: Organic Letters
https://www.readbyqxmd.com/read/27105283/a-multifunctional-monooxygenase-xano4-catalyzes-xanthone-formation-in-xantholipin-biosynthesis-via-a-cryptic-demethoxylation
#17
Lingxin Kong, Weike Zhang, Yit Heng Chooi, Lu Wang, Bo Cao, Zixin Deng, Yiwen Chu, Delin You
Xantholipin and several related polycyclic xanthone antibiotics feature a unique xanthone ring nucleus within a highly oxygenated, angular, fused hexacyclic system. In this study, we demonstrated that a flavin-dependent monooxygenase (FMO) XanO4 catalyzes the oxidative transformation of an anthraquinone to a xanthone system during the biosynthesis of xantholipin. In vitro isotopic labeling experiments showed that the reaction involves sequential insertion of two oxygen atoms, accompanied by an unexpected cryptic demethoxylation reaction...
April 21, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/27099957/heterologous-production-of-fungal-maleidrides-reveals-the-cryptic-cyclization-involved-in-their-biosynthesis
#18
Katherine Williams, Agnieszka J Szwalbe, Nicholas P Mulholland, Jason L Vincent, Andrew M Bailey, Christine L Willis, Thomas J Simpson, Russell J Cox
Fungal maleidrides are an important family of bioactive secondary metabolites that consist of 7, 8, or 9-membered carbocycles with one or two fused maleic anhydride moieties. The biosynthesis of byssochlamic acid (a nonadride) and agnestadride A (a heptadride) was investigated through gene disruption and heterologous expression experiments. The results reveal that the precursors for cyclization are formed by an iterative highly reducing fungal polyketide synthase supported by a hydrolase, together with two citrate-processing enzymes...
June 1, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27094768/structural-basis-for-the-selective-binding-of-inhibitors-to-6-hydroxymethyl-7-8-dihydropterin-pyrophosphokinase-from-staphylococcus-aureus-and-escherichia-coli
#19
Matthew L Dennis, Noel P Pitcher, Michael D Lee, Aaron J DeBono, Zhong-Chang Wang, Jitendra R Harjani, Raphaël Rahmani, Ben Cleary, Thomas S Peat, Jonathan B Baell, James D Swarbrick
6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) is a member of the folate biosynthesis pathway found in prokaryotes and lower eukaryotes that catalyzes the pyrophosphoryl transfer from the ATP cofactor to a 6-hydroxymethyl-7,8-dihydropterin substrate. We report the chemical synthesis of a series of S-functionalized 8-mercaptoguanine (8MG) analogues as substrate site inhibitors of HPPK and quantify binding against the E. coli and S. aureus enzymes (EcHPPK and SaHPPK). The results demonstrate that analogues incorporating acetophenone-based substituents have comparable affinities for both enzymes...
June 9, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27072538/transcriptomic-and-metabolomic-profiling-of-ionic-liquid-stimuli-unveils-enhanced-secondary-metabolism-in-aspergillus-nidulans
#20
Paula C Alves, Diego O Hartmann, Oscar Núñez, Isabel Martins, Teresa L Gomes, Helga Garcia, Maria Teresa Galceran, Richard Hampson, Jörg D Becker, Cristina Silva Pereira
BACKGROUND: The inherent potential of filamentous fungi, especially of Ascomycota, for producing diverse bioactive metabolites remains largely silent under standard laboratory culture conditions. Innumerable strategies have been described to trigger their production, one of the simplest being manipulation of the growth media composition. Supplementing media with ionic liquids surprisingly enhanced the diversity of extracellular metabolites generated by penicillia. This finding led us to evaluate the impact of ionic liquids' stimuli on the fungal metabolism in Aspergillus nidulans and how it reflects on the biosynthesis of secondary metabolites (SMs)...
April 12, 2016: BMC Genomics
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