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https://www.readbyqxmd.com/read/28448554/evaluation-of-efficiency-of-controlled-pollination-based-parentage-analysis-in-a-larix-gmelinii-var-principis-rupprechtii-mayr-seed-orchard
#1
Wenting Sun, Dade Yu, Mingliang Dong, Jian Zhao, Xiaoping Wang, Hongjing Zhang, Jinfeng Zhang
Controlled pollination (CP) is an important tool for breeding programs to improve seed quality, as it rapidly generates desirable genotypes and maximizes genetic gains. However, few studies have evaluated the success rate of CP, especially in Larix gmelinii var. principis-rupprechtii Mayr. seed orchards. In this study, we estimated the rate of correct parentage in 257 CP progeny in an L. gmelinii var. principis-rupprechtii seed orchard from ten candidate parents using 13 microsatellites. The parentage exclusion probabilities of all combined loci in the single parent and parent pair tests were > 0...
2017: PloS One
https://www.readbyqxmd.com/read/28448211/prevalence-and-geographic-distribution-of-vector-borne-pathogens-in-apparently-healthy-dogs-in-croatia
#2
Vladimir Mrljak, Josipa Kuleš, Željko Mihaljević, Marin Torti, Jelena Gotić, Martina Crnogaj, Tatjana Živičnjak, Iva Mayer, Iva Šmit, Mangesh Bhide, Renata Barić Rafaj
Vector-borne pathogens (VBPs) are a group of globally extended and quickly spreading pathogens that are transmitted by various arthropod vectors. The aim of the present study was to investigate the seroprevalence against Babesia canis, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Leishmania infantum, Dirofilaria immitis, and Ehrlichia canis in dogs in Croatia. We investigated 435 randomly selected apparently healthy dogs in 13 different locations of Croatia for antibodies to B. canis by indirect immunofluorescence using a commercial IFA IgG Antibody Kit...
April 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28448051/protocols-for-investigating-the-host-tissue-distribution-transmission-mode-and-effect-on-the-host-fitness-of-a-densovirus-in-the-cotton-bollworm
#3
Xianming Yang, Pengjun Xu, Robert I Graham, He Yuan, Kongming Wu
Many novel viruses have been discovered in animal hosts using next-generation sequencing technologies. Previously, we reported a mutualistic virus, Helicoverpa armigera densovirus (HaDV2), in a lepidopteran species, the cotton bollworm, Helicoverpa armigera (Hubner). Here, we describe the protocols that are currently used to study the effect of HaDV2 on its host. First, we establish a HaDV2-free cotton bollworm colony from a single breeding pair. Then, we orally inoculate some neonate larval offspring with HaDV2-containing filtered liquid to produce two colonies with the same genetic background: one HaDV2-infected, the other uninfected...
April 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447635/a-chromosome-conformation-capture-ordered-sequence-of-the-barley-genome
#4
Martin Mascher, Heidrun Gundlach, Axel Himmelbach, Sebastian Beier, Sven O Twardziok, Thomas Wicker, Volodymyr Radchuk, Christoph Dockter, Pete E Hedley, Joanne Russell, Micha Bayer, Luke Ramsay, Hui Liu, Georg Haberer, Xiao-Qi Zhang, Qisen Zhang, Roberto A Barrero, Lin Li, Stefan Taudien, Marco Groth, Marius Felder, Alex Hastie, Hana Šimková, Helena Staňková, Jan Vrána, Saki Chan, María Muñoz-Amatriaín, Rachid Ounit, Steve Wanamaker, Daniel Bolser, Christian Colmsee, Thomas Schmutzer, Lala Aliyeva-Schnorr, Stefano Grasso, Jaakko Tanskanen, Anna Chailyan, Dharanya Sampath, Darren Heavens, Leah Clissold, Sujie Cao, Brett Chapman, Fei Dai, Yong Han, Hua Li, Xuan Li, Chongyun Lin, John K McCooke, Cong Tan, Penghao Wang, Songbo Wang, Shuya Yin, Gaofeng Zhou, Jesse A Poland, Matthew I Bellgard, Ljudmilla Borisjuk, Andreas Houben, Jaroslav Doležel, Sarah Ayling, Stefano Lonardi, Paul Kersey, Peter Langridge, Gary J Muehlbauer, Matthew D Clark, Mario Caccamo, Alan H Schulman, Klaus F X Mayer, Matthias Platzer, Timothy J Close, Uwe Scholz, Mats Hansson, Guoping Zhang, Ilka Braumann, Manuel Spannagl, Chengdao Li, Robbie Waugh, Nils Stein
Cereal grasses of the Triticeae tribe have been the major food source in temperate regions since the dawn of agriculture. Their large genomes are characterized by a high content of repetitive elements and large pericentromeric regions that are virtually devoid of meiotic recombination. Here we present a high-quality reference genome assembly for barley (Hordeum vulgare L.). We use chromosome conformation capture mapping to derive the linear order of sequences across the pericentromeric space and to investigate the spatial organization of chromatin in the nucleus at megabase resolution...
April 26, 2017: Nature
https://www.readbyqxmd.com/read/28447394/estimation-of-maintenance-energy-requirements-in-german-shepherd-and-labrador-retriever-dogs-in-bangalore-india
#5
H S Madhusudhan, K Chandrapal Singh, U Krishnamoorthy, K G Umesh, R Butterwick, D Wrigglesworth
Maintenance energy requirements (MERs) were calculated for 17 German shepherd and 20 Labrador retriever adult dogs using an in-home prospective dietary trial. The dogs were fed the same dry pet food and body weight, food intake, body condition score and physical activity were monitored for 10 weeks. Labrador retrievers were significantly heavier and had higher body condition scores than German shepherd dogs, but there was no difference between males and females within each group. Body weights remained stable over the study period, with an average daily gain of 9...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28447195/salivary-mir-16-mir-191-and-mir-223-intuitive-indicators-of-dominant-ovarian-follicles-in-buffaloes
#6
Prashant Singh, Naresh Golla, Pankaj Singh, Vijay Simha Baddela, Subhash Chand, Rubina Kumari Baithalu, Dheer Singh, Suneel Kumar Onteru
Estrus or sexual receptivity determination is utmost important for efficient breeding programs for female buffaloes. Prominent estrus behavioral symptoms are the result of several molecular and neuroendocrine events involving the ovary and the brain. Expression of estrus behavior is poor in buffaloes during the summer season. Hence, the discovery of biomarkers specific to the estrus stage or its related ovarian events, like the presence of dominant ovarian follicle, is helpful for developing an easy estrus determination method...
April 26, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28446955/combination-of-cyclamen-persicum-mill-floral-gene-promoters-and-chimeric-repressors-for-the-modification-of-ornamental-traits-in-torenia-fournieri-lind
#7
Ichiro Kasajima, Norihiro Ohtsubo, Katsutomo Sasaki
Although chimeric repressors such as the Arabidopsis TCP3 repressor are known to have significant effects on flower morphology and color, their cellular-level effects on flower petals are not understood. The promoter sequences of the genes expressed in the flowers of cyclamen, a representative potted flower grown during the winter season, are also unknown. Here, we isolated eight promoters from cyclamen genes that are reportedly expressed in the petals. These promoters were then fused to four chimeric repressors and introduced into the model flower torenia to screen for effective combinations of promoters and repressors for flower breeding...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28446772/large-scale-snp-screenings-identify-markers-linked-with-gcrv-resistant-traits-through-transcriptomes-of-individuals-and-cell-lines-in-ctenopharyngodon-idella
#8
Zhiwei Liao, Quanyuan Wan, Xueying Shang, Jianguo Su
Grass carp (Ctenopharyngodon idella) is an important economic species in freshwater aquaculture and its industry has been confined due to variety degeneration and frequent diseases. Marker-assisted selection is a feasible method for selective breeding of new varieties. Transcriptome data have greatly facilitated high-throughput single nucleotide polymorphism (SNP) marker discovery and phenotype association study. In this study, we gained a total of 25,981 and 5,775 high quality SNPs in two transcriptomes from individuals and cell lines, respectively...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28446701/wind-and-rain-are-the-primary-climate-factors-driving-changing-phenology-of-an-aerial-insectivore
#9
Rachel D Irons, April Harding Scurr, Alexandra P Rose, Julie C Hagelin, Tricia Blake, Daniel F Doak
While the ecological effects of climate change have been widely observed, most efforts to document these impacts in terrestrial systems have concentrated on the impacts of temperature. We used tree swallow (Tachycineta bicolor) nest observations from two widely separated sites in central Alaska to examine the aspects of climate affecting breeding phenology at the northern extent of this species' range. We found that two measures of breeding phenology, annual lay and hatch dates, are more strongly predicted by windiness and precipitation than by temperature...
April 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28446328/combined-gc-and-uhplc-hr-ms-based-metabolomics-to-analyze-durable-anti-fungal-resistance-processes-in-cereals
#10
Rahel Bucher, Daniel Veyel, Lothar Willmitzer, Simon Krattinger, Beat Keller, Laurent Biglera
Introduction of durable resistance genes in crops is an important strategy to prevent yield loss caused by pathogens. The durable multi-pathogen resistance gene Lr34 originating from wheat is widely used in breeding, and is functionally transferable to barley and rice. The molecular resistance mechanism of Lr34, encoding for an adenosine triphosphate-binding cassette transporter, is not known yet. To understand the molecular function and the defense response of durable disease resistance in cereals, the metabolic response of Lr34 was investigated in, except for the Lr34 gene, genetically identical lines of barley, rice and wheat...
April 26, 2017: Chimia
https://www.readbyqxmd.com/read/28446247/difference-in-oxidative-stress-tolerance-between-rice-cultivars-estimated-with-chlorophyll-fluorescence-analysis
#11
Ichiro Kasajima
BACKGROUND: Oxidative stress is considered to be involved in growth retardation of plants when they are exposed to a variety of biotic and abiotic stresses. Despite its potential importance in improving crop production, comparative studies on oxidative stress tolerance between rice (Oryza sativa L.) cultivars are limited. This work describes the difference in term of oxidative stress tolerance between 72 rice cultivars. METHODS: 72 rice cultivars grown under naturally lit greenhouse were used in this study...
April 26, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28446214/epidemiological-role-of-dogs-since-the-human-leishmaniosis-outbreak-in-madrid
#12
Guadalupe Miró, Aurora Müller, Ana Montoya, Rocía Checa, Valentina Marino, Eloy Marino, Fernando Fuster, Cristina Escacena, Miguel Angel Descalzo, Rosa Gálvez
BACKGROUND: Canine leishmaniosis (CanL) has been in the spotlight since the 2009 outbreak of human leishmaniosis in Madrid. In the framework of the Leishmaniosis Surveillance Programme set up in Madrid, this study examines Leishmania-specific seroprevalences in stray dogs for the outbreak area and rest of the Madrid region over the period spanning from the outbreak to the present (2009-2016). These data are of interest because stray dogs could be sentinels for disease surveillance in endemic areas...
April 26, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28446022/a-survey-of-anthocyanin-composition-and-concentration-in-diverse-maize-germplasm
#13
Michael Paulsmeyer, Laura Chatham, Talon Becker, Megan West, Leslie West, John Juvik
Increasing consumer demand for natural ingredients in foods and beverages justifies investigations into more economic sources of natural colorants. In this study, 398 genetically diverse pigmented accessions of maize were analyzed using HPLC to characterize the diversity of anthocyanin composition and concentration in maize germplasm. 167 accessions were identified that could produce anthocyanins in the kernel pericarp or aleurone and were classified into compositional categories. Total anthocyanin content was highest in pericarp-pigmented accessions with flavanol-anthocyanin condensed forms, similar to the Andean Maíz Morado landraces...
April 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28446001/a-post-genome-wide-association-study-validating-the-association-of-the-glycophorin-c-gene-with-serum-hemoglobin-level-in-pig
#14
Yang Liu, Zhengzheng Hu, Chen Yang, Shiwei Wang, Wenwen Wang, Qin Zhang
OBJECTIVE: This study aimed to validate the statistical evidence from the genome-wide association study (GWAS) as true-positive and to better understand the effects of the glycophorin C (GYPC) gene on serum hemoglobin traits. METHODS: Our initial GWAS revealed the presence of two single nucleotide polymorphisms (SNPs) (ASGA0069038 and ALGA0084612) for the hemoglobin concentration trait (HGB) in the 2.48 Mb region of SSC15. From this target region, GYPC was selected as a promising gene that associated with serum HGB traits in pigs...
May 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28445722/genomic-analyses-reveal-the-influence-of-geographic-origin-migration-and-hybridization-on-modern-dog-breed-development
#15
Heidi G Parker, Dayna L Dreger, Maud Rimbault, Brian W Davis, Alexandra B Mullen, Gretchen Carpintero-Ramirez, Elaine A Ostrander
There are nearly 400 modern domestic dog breeds with a unique histories and genetic profiles. To track the genetic signatures of breed development, we have assembled the most diverse dataset of dog breeds, reflecting their extensive phenotypic variation and heritage. Combining genetic distance, migration, and genome-wide haplotype sharing analyses, we uncover geographic patterns of development and independent origins of common traits. Our analyses reveal the hybrid history of breeds and elucidate the effects of immigration, revealing for the first time a suggestion of New World dog within some modern breeds...
April 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28445532/use-of-molecular-markers-in-identification-and-characterization-of-resistance-to-rice-blast-in-india
#16
Manoj Kumar Yadav, Aravindan S, Umakanta Ngangkham, H N Shubudhi, Manas Kumar Bag, Totan Adak, Sushmita Munda, Sanghamitra Samantaray, Mayabini Jena
Rice blast disease caused by Magnaporthe oryzae is one of the most destructive disease causing huge losses to rice yield in different parts of the world. Therefore, an attempt has been made to find out the resistance by screening and studying the genetic diversity of eighty released rice varieties by National Rice Research Institute, Cuttack (NRVs) using molecular markers linked to twelve major blast resistance (R) genes viz Pib, Piz, Piz-t, Pik, Pik-p, Pikm Pik-h, Pita/Pita-2, Pi2, Pi9, Pi1 and Pi5. Out of which, nineteen varieties (23...
2017: PloS One
https://www.readbyqxmd.com/read/28445423/anthocyanin-profiles-in-flowers-of-grape-hyacinth
#17
Qian Lou, Lin Wang, Hongli Liu, Yali Liu
Grape hyacinth (Muscari spp.) is a popular ornamental bulbous perennial famous for its blue flowers. To understand the chemical basis of the rich blue colors in this plant, anthocyanin profiles of six blue flowering grape hyacinths as well as one pink and one white cultivar were determined using high-performance liquid chromatography and mass spectrometry. Along with two known compounds, eight putative anthocyanins were identified in the tepals of grape hyacinth for the first time. The accumulation and distribution of anthocyanins in the plant showed significant cultivar and flower development specificity...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28444838/modified-brow-suspension-technique-for-the-treatment-of-pseudoptosis-due-to-redundant-frontal-skin-in-the-dog-a-retrospective-study-of-25-cases
#18
Martí Cairó, Marta Leiva, Daniel Costa, Maria Teresa Peña
PURPOSE: To describe the outcome of modified brow suspension technique for the treatment of upper eyelid pseudoptosis in canine patients with redundant frontal skin folds. METHODS: Medical records from 1999 to 2015 were retrospectively reviewed. Only dogs that underwent modified brow suspension for treating bilateral pseudoptosis were included in the study. Recorded data included breed, age, gender, main ophthalmic complaint, ocular findings, suture material used for brow suspension, number of slings placed, postoperative treatment, complications, follow-up time, and outcome...
April 25, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28444756/e7-1057%C3%AE-ta-mutation-of-the-acidic-%C3%AE-glucosidase-gene-causes-pompe-s-disease-in-droughtmaster-cattle
#19
R E Lyons, D J Johnston, M R McGowan, A Laing, B Robinson, H Owen, B D Hill, B M Burns
OBJECTIVE: To determine whether known loss-of-function alleles of the acidic α-glucosidase gene (GAA) are present in the Droughtmaster breed and, if so, whether the clinical signs and pathology of generalised glycogenosis (Pompe's disease) previously reported in other affected cattle are also seen in homozygous Droughtmasters. DESIGN: Existing genomic and other diagnostic tests developed for generalised glycogenosis in cattle were used to test for the presence of the three known loss-of-function alleles of GAA in a herd of Droughtmaster cattle...
May 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28444754/retrospective-study-on-the-occurrence-of-canine-lymphoma-and-associated-breed-risks-in-a-population-of-dogs-in-nsw-2001-2009
#20
Ppy Yau, N K Dhand, P C Thomson, R M Taylor
OBJECTIVE: To identify risk factors for canine lymphoma in dogs from New South Wales, Australia, and to compare factors affecting remission duration. METHODS: Client-owned dogs diagnosed with lymphoma presented to the University Veterinary Teaching Hospital (UVTH), University of Sydney, between 2001 and 2009 (n = 134) were compared with a control population of dogs seen in that period of time with a diagnosis other than lymphoma to evaluate association of explanatory variables (breed, age and sex) with the outcome (case or control status)...
May 2017: Australian Veterinary Journal
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