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https://www.readbyqxmd.com/read/28342078/an-anoxic-aerobic-system-for-simultaneous-biodegradation-of-phenol-and-ammonia-in-a-sequencing-batch-reactor
#1
Qifeng Liu, Vijay P Singh, Zhimin Fu, Jing Wang, La Hu
A laboratory-scale sequencing batch reactor (SBR) was investigated to treat artificial pretreated coal gasification wastewater that was mainly contained of ammonia and phenol. The efficiency of SBR fed with increasing phenol concentrations (from 150 to 300 mg l(-1)) and the relationship among phenol, nitrogen removal, and the microbial community structure were evaluated. When the phenol feeding concentration was increased to about 300 mg l(-1), the removal efficiency was above 99.0%, demonstrating the robustness of phenol removal capacity...
March 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28340435/optimization-of-hydrogen-dispersion-in-thermophilic-up-flow-reactors-for-ex-situ-biogas-upgrading
#2
Ilaria Bassani, Panagiotis G Kougias, Laura Treu, Hugo Porté, Stefano Campanaro, Irini Angelidaki
This study evaluates the efficiency of four novel up-flow reactors for ex situ biogas upgrading converting externally provided CO2 and H2 to CH4, via hydrogenotrophic methanogenesis. The gases were injected through stainless steel diffusers combined with alumina ceramic sponge or through alumina ceramic membranes. Pore size, input gas loading and gas recirculation flow rate were modulated to optimize gas-liquid mass transfer, and thus methanation efficiency. Results showed that larger pore size diffusion devices achieved the best kinetics and output-gas quality converting all the injected H2 and CO2, up to 3...
March 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28334664/high-rate-nitrogen-removal-by-anammox-internal-circulation-reactor-ic-for-old-landfill-leachate-treatment
#3
The Nhat Phan, Thi Thanh Van Truong, Nhu Biec Ha, Phuoc Dan Nguyen, Xuan Thanh Bui, Bao Trong Dang, Van Tuan Doan, Joonhong Park, Wenshan Guo, Huu Hao Ngo
This study aimed to evaluate the performance of a high rate nitrogen removal lab-scale ANAMMOX reactor, namely Internal Circulation (IC) reactor, for old landfill leachate treatment. The reactor was operated with pre-treated leachate from a pilot Partial Nitritation Reactor (PNR) using a high nitrogen loading rate ranging from 2 to 10kgNm(-3)d(-1). High rate removal of nitrogen (9.52±1.11kgNm(-3)d(-1)) was observed at an influent nitrogen concentration of 1500mgNL(-1). The specific ANAMMOX activity was found to be 0...
February 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28333264/single-cell-transcriptomics-mega-phylogeny-and-the-genetic-basis-of-morphological-innovations-in-rhizaria
#4
Anders K Krabberød, Russell J S Orr, Jon Bråte, Tom Kristensen, Kjell R Bjørklund, Kamran Shalchian-Tabrizi
The innovation of the eukaryote cytoskeleton enabled phagocytosis, intracellular transport and cytokinesis, and is responsible for the diversity of eukaryotic morphologies. Still, the relationship between phenotypic innovations in the cytoskeleton and their underlying genotype is poorly understood. To explore the genetic mechanism of morphological evolution of the eukaryotic cytoskeleton we provide the first single cell transcriptomes from uncultured, free-living unicellular eukaryotes: the polycystine radiolarian Lithomelissa setosa (Nassellaria) and Sticholonche zanclea (Taxopodida)...
March 1, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28330619/efficient-ex%C3%A2-vivo-engineering-and-expansion-of-highly-purified-human-hematopoietic-stem-and-progenitor-cell-populations-for-gene-therapy
#5
Erika Zonari, Giacomo Desantis, Carolina Petrillo, Francesco E Boccalatte, Maria Rosa Lidonnici, Anna Kajaste-Rudnitski, Alessandro Aiuti, Giuliana Ferrari, Luigi Naldini, Bernhard Gentner
Ex vivo gene therapy based on CD34(+) hematopoietic stem cells (HSCs) has shown promising results in clinical trials, but genetic engineering to high levels and in large scale remains challenging. We devised a sorting strategy that captures more than 90% of HSC activity in less than 10% of mobilized peripheral blood (mPB) CD34(+) cells, and modeled a transplantation protocol based on highly purified, genetically engineered HSCs co-infused with uncultured progenitor cells. Prostaglandin E2 stimulation allowed near-complete transduction of HSCs with lentiviral vectors during a culture time of less than 38 hr, mitigating the negative impact of standard culture on progenitor cell function...
March 13, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28330207/a-pmoa-based-study-reveals-dominance-of-yet-uncultured-type-i-methanotrophs-in-rhizospheres-of-an-organically-fertilized-rice-field-in-india
#6
Pranitha S Pandit, Dilip R Ranade, Prashant K Dhakephalkar, Monali C Rahalkar
Rice fields are one of the important sources of anthropogenic methane. Methanotrophs can oxidize up to 30 % of the produced methane and thus have a pivotal environmental role in methane mitigation. India occupies the largest region under rice cultivation; however, most of the studies done on methanotrophic communities have focused on the Northern region. We studied methanotrophic community of a flooded, organically fertilized rice field using pmoA clone library approach. Organic rice fields impose a more serious threat as they produce more methane...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330142/an-overview-of-physico-chemical-mechanisms-of-biogas-production-by-microbial-communities-a-step-towards-sustainable-waste-management
#7
REVIEW
Ramansu Goswami, Pritam Chattopadhyay, Arunima Shome, Sambhu Nath Banerjee, Amit Kumar Chakraborty, Anil K Mathew, Shibani Chaudhury
Biogas is a combination of methane, CO2, nitrogen, H2S and traces of few other gases. Almost any organic waste can be biologically transformed into biogas and other energy-rich organic compounds through the process of anaerobic digestion (AD) and thus helping in sustainable waste management. Although microbes are involved in each step of AD, knowledge about those microbial consortia is limited due to the lack of phylogenetic and metabolic data of predominantly unculturable microorganisms. However, culture-independent methods like PCR-based ribotyping has been successfully employed to get information about the microbial consortia involved in AD...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330126/characterization-of-lignin-degrading-enzymes-ldes-from-a-dimorphic-novel-fungus-and-identification-of-products-of-enzymatic-breakdown-of-lignin
#8
Lipin Dev Mundur Sahadevan, Chandra Shekhar Misra, V Thankamani
Lignin is a major component of all plants, the degradation of which remains a major challenge to date owing to its recalcitrant nature. Several classes of fungi have been studied to carry out this process to some extent, but overall the process remains inefficient. We have isolated a novel alkalophilic dimorphic lignin-degrading Deuteromycete from soil, identified as "uncultured" and coded as MVI.2011. Supernatant from 12-h culture of MVI.2011 in optimized mineral medium containing lignin pH 9.0 was analysed for Lignin Peroxidase, Manganese Peroxidase and Laccase...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330093/exploration-of-nifh-gene-through-soil-metagenomes-of-the-western-indian-himalayas
#9
Ravindra Soni, Deep Chandra Suyal, Santosh Sai, Reeta Goel
This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by polymerase chain reaction (PCR) amplification. After preliminary screening, selected clones were sequenced. In silico analysis of the clones was done, which documented 83...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328934/proviruses-with-identical-sequences-comprise-a-large-fraction-of-the-replication-competent-hiv-reservoir
#10
John K Bui, Michele D Sobolewski, Brandon F Keele, Jonathan Spindler, Andrew Musick, Ann Wiegand, Brian T Luke, Wei Shao, Stephen H Hughes, John M Coffin, Mary F Kearney, John W Mellors
The major obstacle to curing HIV infection is the persistence of cells with intact proviruses that can produce replication-competent virus. This HIV reservoir is believed to exist primarily in CD4+ T-cells and is stable despite years of suppressive antiretroviral therapy. A potential mechanism for HIV persistence is clonal expansion of infected cells, but how often such clones carry replication-competent proviruses has been controversial. Here, we used single-genome sequencing to probe for identical HIV sequence matches among viruses recovered in different viral outgrowth cultures and between the sequences of outgrowth viruses and proviral or intracellular HIV RNA sequences in uncultured blood mononuclear cells from eight donors on suppressive ART with diverse proviral populations...
March 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28325151/adipose-tissue-derived-pericytes-for-cartilage-tissue-engineering
#11
Jin Xin Zhang, Chun Yan Du, Wei Min Guo, Pan Li, Shu Yun Liu, Zhi Guo Yuan, Jian Hua Yang, Xun Sun, He Yong Yin, Quan Yi Guo, Chen Fu Zhou
Mesenchymal stem cells (MSCs) represent a promising alternative source for cartilage tissue engineering. However, MSC culture is labor-intensive, so these cells cannot be applied immediately to regenerate cartilage for clinical purposes. Risks during the ex vivo expansion of MSCs, such as infection and immunogenicity, can be a bottleneck in their use in clinical tissue engineering. As a novel stem cell source, pericytes are generally considered to be the origin of MSCs. Pericytes do not have to undergo time-consuming ex vivo expansion because they are uncultured cells...
March 21, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28321126/genomic-reconstruction-of-multiple-lineages-of-uncultured-benthic-archaea-suggests-distinct-biogeochemical-roles-and-ecological-niches
#12
Cassandre S Lazar, Brett J Baker, Kiley W Seitz, Andreas P Teske
No abstract text is available yet for this article.
April 2017: ISME Journal
https://www.readbyqxmd.com/read/28319019/recycling-of-metals-from-pretreated-waste-printed-circuit-boards-effectively-in-stirred-tank-reactor-by-a-moderately-thermophilic-culture
#13
Ming-Chen Xia, Ya-Ping Wang, Tang-Jian Peng, Li Shen, Run-Lan Yu, Yuan-Dong Liu, Miao Chen, Jiao-Kun Li, Xue-Ling Wu, Wei-Min Zeng
To seek a feasible technique for processing waste printed circuit boards (PCBs), pretreatment of PCBs by table separation and further bioleached by moderate thermophiles in a stirred tank reactor were investigated. The shaking table separation, conducted after grinding and sieving of PCBs, produced two fractions: metal-rich parts (RPCBs), which is more suitable for pyrometallurgy process than untreated PCBs, and metal-poor parts (PPCBs) with only 8.83% metals was then bioleached by a mixed culture of moderate thermophiles effectively...
March 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28314726/global-distribution-patterns-and-pangenomic-diversity-of-the-candidate-phylum-latescibacteria-ws3
#14
Ibrahim F Farag, Noha H Youssef, Mostafa S Elshahed
We investigated the global distribution patterns and pangenomic diversity of the candidate phylum "Latescibacteria" (WS3) in 16S rRNA gene as well as metagenomic datasets. We document distinct distribution patterns for various "Latescibacteria" orders in 16S rRNA gene datasets, with prevalence of orders sediment_1 in terrestrial, PBSIII_9 in groundwater and temperate freshwater, and GN03 in pelagic marine, saline-hypersaline, and wastewater habitats. Using a fragment recruitment approach, we identified 68.9 Mb of "Latescibacteria"-affiliated contigs in publicly available metagenomic datasets comprising 73,079 proteins...
March 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28300526/effects-of-bone-morphogenetic-protein-4-bmp4-on-in-vitro-development-and-survival-of-bovine-preantral-follicles-enclosed-in-fragments-ovarian-tissue
#15
Ellen de Vasconcelos da Cunha, Glaucinete Borges de Souza, José Renato de Sousa Passos, Anderson Weiny Barbalho Silva, Andressa Minussi Dau, Márcia Viviane Alves Saraiva, Raimundo Nonato Braga Lobo, José Roberto Viana Silva
The aim of this study was to evaluate the effects of different concentrations of BMP4 on activation, development and mRNA expression of GDF9, BMP15, PCNA, Bax and Bcl2 in cultured bovine follicles enclosed in ovarian tissues. Ovarian tissue fragments were cultured for 6 days in α-MEM+ alone or supplemented with different concentrations of BMP4 (10, 50 or 100 ng/ml). Classical histology was performed to analyze follicle growth and morphology, while real-time PCR was used to analyze mRNA levels in fresh and cultured tissues...
March 16, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28285498/profiling-total-viable-bacteria-in-a-hemodialysis-water-treatment-system
#16
Lihua Chen, Xuan Zhu, Menglu Zhang, Yuxin Wang, Tianyu Lv, Shenghua Zhang, Xin Yu
The culture-dependent methods such as heterotrophic plate counting (HPC) are usually applied to evaluate the bacteriological quality of the hemodialysis water. However, these methods cannot detect the uncultured or viable but non-culturable (VBNC) bacteria both of which may be quantitatively predominant throughout the hemodialysis water treatment system. Therefore, PMA-qPCR associated with HPC was used together to profile the distribution of the total viable bacteria in such a system. Moreover, high-throughput sequencing of 16s rRNA gene amplicons was utilized to analyze the microbial community structure and diversity...
March 13, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28265709/comparison-of-prokaryotic-diversity-in-cold-oligotrophic-remote-lakes-of-chilean-patagonia
#17
Paulina Aguayo, Paulina González, Víctor Campos, Teresa L Maugeri, Maria Papale, Concetta Gugliandolo, Miguel A Martinez
The prokaryotic abundance and diversity in three cold, oligotrophic Patagonian lakes (Témpanos, Las Torres and Mercedes) in the northern region Aysén (Chile) were compared in winter and summer using 16S rRNA fluorescence in situ hybridization and PCR-denaturing gradient gel electrophoresis technique. Prokaryotic abundances, numerically dominated by Bacteria, were quite similar in the three lakes, but higher in sediments than in waters, and they were also higher in summer than in winter. The relative contribution of Archaea was greater in waters than in sediments, and in winter rather than in summer...
March 7, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28265558/a-clostridium-group-iv-species-dominates-and-suppresses-a-mixed-culture-fermentation-by-tolerance-to-medium-chain-fatty-acids-products
#18
Stephen J Andersen, Vicky De Groof, Way Cern Khor, Hugo Roume, Ruben Props, Marta Coma, Korneel Rabaey
A microbial community is engaged in a complex economy of cooperation and competition for carbon and energy. In engineered systems such as anaerobic digestion and fermentation, these relationships are exploited for conversion of a broad range of substrates into products, such as biogas, ethanol, and carboxylic acids. Medium chain fatty acids (MCFAs), for example, hexanoic acid, are valuable, energy dense microbial fermentation products, however, MCFA tend to exhibit microbial toxicity to a broad range of microorganisms at low concentrations...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28264042/phylogenetic-diversity-and-in-situ-detection-of-eukaryotes-in-anaerobic-sludge-digesters
#19
Miri Matsubayashi, Yusuke Shimada, Yu-You Li, Hideki Harada, Kengo Kubota
Eukaryotic communities in aerobic wastewater treatment processes are well characterized, but little is known about them in anaerobic processes. In this study, abundance, diversity and morphology of eukaryotes in anaerobic sludge digesters were investigated by quantitative real-time PCR (qPCR), 18S rRNA gene clone library construction and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Samples were taken from four different anaerobic sludge digesters in Japan. Results of qPCR of rRNA genes revealed that Eukarya accounted from 0...
2017: PloS One
https://www.readbyqxmd.com/read/28261669/environmental-viral-genomes-shed-new-light-on-virus-host-interactions-in-the-ocean
#20
Yosuke Nishimura, Hiroyasu Watai, Takashi Honda, Tomoko Mihara, Kimiho Omae, Simon Roux, Romain Blanc-Mathieu, Keigo Yamamoto, Pascal Hingamp, Yoshihiko Sako, Matthew B Sullivan, Susumu Goto, Hiroyuki Ogata, Takashi Yoshida
Metagenomics has revealed the existence of numerous uncharacterized viral lineages, which are referred to as viral "dark matter." However, our knowledge regarding viral genomes is biased toward culturable viruses. In this study, we analyzed 1,600 (1,352 nonredundant) complete double-stranded DNA viral genomes (10 to 211 kb) assembled from 52 marine viromes. Together with 244 previously reported uncultured viral genomes, a genome-wide comparison delineated 617 genus-level operational taxonomic units (OTUs) for these environmental viral genomes (EVGs)...
March 2017: MSphere
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