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https://www.readbyqxmd.com/read/28738670/antidoping-program-an-important-factor-in-the-promotion-and-protection-of-the-integrity-of-sport-and-athlete-s-health
#1
Filomena Mazzeo, Vincenzo Monda, Stefania Santamaria, Ersilia Nigro, Anna Valenzano, Ines Villano, Giuseppe Cibelli, Antonietta Messina, Giovanni Messina
Use of performance-enhancing drugs concern not only elite Olympic and Paralympic Games' athletes but also amateur athletes, who are making increasing use of substances and/or methods. Furthermore, a new frontier reached by the doping is the use of genes. World Anti- Doping Agency (WADA), expressly prohibited the participation in competitive sports by the athlete in case of taking banned substances to treat disease in the event that the above assumption implies an excessive improvement of performance. This study aims to analyze and show the doping control as an essential part of the anti-doping program to promote and protect the integrity of sport and athlete's health...
July 24, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/28738664/bomb-swab-can-trace-explosive-particle-sampling-and-detection-be-improved
#2
Danny Fisher, Raya Zach, Yossef Matana, Paz Elia, Shiran Shustack, Yarden Sharon, Yehuda Zeiri
The marked increase in international terror in recent years requires the development of highly efficient methods to detect trace amounts of explosives at airports, border crossings and check points. The preferred analytical method worldwide is the ion mobility spectrometry (IMS) that is capable of detecting most explosives at the nano-gram level. Sample collection for the IMS analysis is based on swabbing of a passenger's belongings to collect possible explosive residues. The present study examines a wide range of issues related to swab-based particle collection and analysis, in the hope of gaining deeper understanding into this technique that will serve to improve the detection process...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738663/statistical-approach-for-atr-ftir-screening-of-semen-in-sexual-evidence
#3
Inês Gregório, Félix Zapata, Mercedes Torre, Carmen García-Ruiz
Genetic identification has revolutionized the Forensic Sciences, especially in sexual aggression cases. For the successful extraction of the genetic information of a criminal, a crucial step is the prior detection of bodily fluids on evidence. In this article, a method for non-destructive screening of semen samples is reported. Using chemometric tools, bodily fluids can be detected and differentiated without damaging the sample, by correlating the infrared spectra of sexual evidence with previously recorded spectra from undamaged stains of individual bodily fluids...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738661/semiautomatic-sequential-extraction-of-polycyclic-aromatic-hydrocarbons-and-elemental-bio-accessible-fraction-by-accelerated-solvent-extraction-on-a-single-particulate-matter-sample
#4
Maria Luisa Astolfi, Patrizia Di Filippo, Alessandra Gentili, Silvia Canepari
We describe the optimization and validation of a sequential extractive method for the determination of the polycyclic aromatic hydrocarbons (PAHs) and elements (Al, As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Se, V and Zn) that are chemically fractionated into bio-accessible and mineralized residual fractions on a single particulate matter filter. The extraction is performed by automatic accelerated solvent extraction (ASE); samples are sequentially treated with dichloromethane/acetone (4:1) for PAHs extraction and acetate buffer (0...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738660/novel-biomimetic-enzyme-for-sensitive-detection-of-superoxide-anions
#5
Fang Peng, Tingting Xu, Fan Wu, Chunxue Ma, Yuhong Liu, Jianying Li, Bo Zhao, Chun Mao
Superoxide anion (O2(•-)), one of the most active reactive oxygen species (ROS) in micro-environment of the human body, is involved in some diseases if there is excess O2(•-) associated with oxidative stress. Accurate detection of its concentration has important medical diagnostic significance. In this work, a new electrochemical sensor was designed and fabricated for sensitive detection based on Mn-superoxide dismutase (MnSOD) that decorated onto the surface of magnetic polymeric nanotubes by surface self-assembly processes...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738654/development-of-a-sensitive-and-quantitative-uhplc-ms-ms-method-to-study-the-whole-body-uptake-of-pharmaceuticals-in-zebrafish
#6
Stanislav Kislyuk, Jerome Kroonen, Erwin Adams, Patrick Augustijns, Peter de Witte, Deirdre Cabooter
An analytical procedure to measure the whole-body uptake of pharmaceuticals in zebrafish has been developed using state-of-the-art methodologies. A sample preparation procedure for 9 pharmaceuticals displaying a variety in physicochemical properties was developed using 10-day old zebrafish (TG898). For an efficient homogenization of the samples and subsequent recovery of the compounds of interest, different amounts of organic solvents in combination with acidic modifiers were added to zebrafish samples. Samples were subsequently processed using a powerful bath sonicator and centrifuged...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738653/development-of-an-efficient-method-for-multi-residue-analysis-of-160-pesticides-in-herbal-plant-by-ethyl-acetate-hexane-mixture-with-direct-injection-to-gc-ms-ms
#7
Sherif M Taha, Sohair A Gadalla
A simple and efficient multi residue method was developed, for the analysis of 160 pesticides by GC-MS/MS in herbal plants. The developed method employs pesticide residue extraction by EtAC/ n. hexane (6:4) with a cleanup step using florisil/ PSA mixture. The optimized conditions have resulted in lower co-extracted matrix components than those extracted using EtAC or MeCN (QuEChERS method), according to FTIR and full scan GC/MS analyses. In addition, the developed method (EtAC/ Hexane) eliminates the evaporation step that is usually performed when using MeCN as an extraction solvent prior to the GC-MS/MS injection...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738652/volatile-composition-of-merlot-red-wine-and-its-contribution-to-the-aroma-optimization-and-validation-of-analytical-method
#8
Stefany Grützmann Arcari, Vinicius Caliari, Marla Sganzerla, Helena Teixeira Godoy
A methodology for the determination of volatile compounds in red wine using headspace solid phase microextraction (HS-SPME) combined with gas chromatography-ion trap/ mass spectrometry (GC-IT/MS) and flame ionization detector (GC -FID) was developed, validated and applied to a sample of Brazilian red wine. The optimization strategy was conducted using the Plackett-Burman design for variable selection and central composite rotational design (CCRD). The response surface methodology showed that the performance of the extraction of the volatile compounds using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber is improved with no sample dilution, the addition of 30% NaCl, applying an extraction temperature of 56°C and extraction time of 55min...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738651/recent-advances-in-sample-pre-treatment-for-emerging-methods-in-proteomic-analysis
#9
María Ramos-Payán, Juan A Ocaña-González, Rut M Fernández-Torres, Santiago Maspoch, Miguel Ángel Bello-López
The objective of proteomics is the study of proteins (including their structure elucidation, the characterization of their role in biochemical process, the detection and identification of anomalous modifications in their structures and behavior, etc.) from both a qualitative and quantitative point of view. These studies have to face several difficulties as (a) the intrinsic complexity of protein molecules, (b) the low concentrations of the studied proteins, (c) the complexity of the biological samples (which could lead to interferences from the components of these matrixes), etc...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738650/determination-of-ascorbic-acid-in-serum-samples-by-screen-printed-carbon-electrodes-modified-with-gold-nanoparticles
#10
M Asunción Alonso-Lomillo, Olga Domínguez-Renedo, Abraham Saldaña-Botín, M Julia Arcos-Martínez
This paper shows the ability of simple modified screen-printed devices for the electrochemical detection of ascorbic acid in different samples, including serum samples. Even if the presence of uric acid does not greatly affect the analysis of ascorbic acid using screen-printed carbon electrodes modified by gold nanoparticles, dopamine must be considered an interferent. Thus, the joint determination of the three compounds has been set out using methods of multivariate calibration. The validated models, built by partial least squares regressions, have been used to determine the concentration of ascorbic acid, dopamine and uric acid of spiked serum samples, with successful results...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738648/matrix-interference-evaluation-employing-gc-and-lc-coupled-to-triple-quadrupole-tandem-mass-spectrometry
#11
S Uclés, A Lozano, A Sosa, P Parrilla Vázquez, A Valverde, A R Fernández-Alba
Gas and liquid chromatography coupled to triple quadrupole tandem mass spectrometry are currently the most powerful tools employed for the routine analysis of pesticide residues in food control laboratories. However, whatever the multiresidue extraction method, there will be a residual matrix effect making it difficult to identify/quantify some specific compounds in certain cases. Two main effects stand out: (i) co-elution with isobaric matrix interferents, which can be a major drawback for unequivocal identification, and therefore false negative detections, and (ii) signal suppression/enhancement, commonly called the "matrix effect", which may cause serious problems including inaccurate quantitation, low analyte detectability and increased method uncertainty...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738645/competitive-fluorescence-anisotropy-polarization-assay-for-atp-using-aptamer-as-affinity-ligand-and-dye-labeled-atp-as-fluorescence-tracer
#12
Yapiao Li, Linlin Sun, Qiang Zhao
We developed an aptamer-based competitive fluorescence anisotropy (FA)/fluorescence polarization (FP) assay for adenosine triphosphate (ATP). Different from the traditional fluorescence polarization immunoassays for small molecules, here DNA aptamer against ATP was used as affinity ligand, and tetramethylrhodamine (TMR) labeled ATP served as fluorescent tracer. The binding between TMR-labeled ATP and aptamer gave large FA due to molecular volume increase and restricted rotation of the dye-labeled ATP. When ATP was added in solution, ATP competitively displaced the TMR-labeled ATP from aptamer affinity complex, causing decrease of FA of TMR-labeled ATP...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738643/facile-preparation-of-3-d-floor-like-ordered-mesoporous-carbon-functionalized-graphene-composites-and-its-application-for-selective-enrichment-of-n-glycans-from-human-serum
#13
Quanqing Zhang, Yuanyu Huang, Haofan Sun, Yangyang Gan, Huali Shen, Bin Jia, Pengyuan Yang
Novel 3-D floor-like ordered mesoporous carbon functionalized graphene composites, FLOMC-GO, with high graphitized carbon contents were successfully synthesized using a soft template method. The one-pot sol-gel method was employed to prepare the silica soft template. Then, the sandwich-like composites were further combined together to form a 3-D structure through pre-carbonization and carbonization. During these procedures, the sulphonyl and sulfide bridges were formed by cross-linking processes to connect the phenyl rings...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738641/an-interference-free-and-label-free-sandwich-type-magnetic-silicon-microsphere-rgo-based-probe-for-fluorescence-detection-of-microrna
#14
Shiyu Li, Kui He, Rong Liao, Chunyan Chen, Xiaoming Chen, Changqun Cai
An interference-free and label-free sensing platform was developed for the highly sensitive detection of microRNA-21 (miRNA-21) in vitro by magnetic silicon microsphere (MNP)-reduced graphene oxide (rGO)-based sandwich probe. In this method, DNA capture probes (P1) were connected with MNPs at the 5' end and hybridized with completely complementary target miRNA. Subsequently, rGO was retained and induced the fluorescence quenching in the supernatant. Through the magnetic separation, the supernatant environment was simplified and the interference to analytical signal was eliminated...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738636/one-step-hydrothermal-synthesis-and-characterization-of-moss-like-mwcnt-bi2s3-nanomaterial-for-solid-phase-extraction-of-copper
#15
Zainab Manzoor Memon, Erkan Yilmaz, Mustafa Soylak
The present work was designed with the aim to one step simple hydrothermal synthesis of moss like MWCNT-Bi2S3 nanomaterial as solid phase extraction sorbent for separation, preconcentration and determination of copper in tobacco and food samples. Cu(II) ions was strongly retained on the MWCNT-Bi2S3 nanomaterial at the pH 7.0 and was successfully desorbed by using 1.5mL of 4M HNO3. The copper concentration in eluent was determined by flame atomic absorption spectrometer. The certified reference material (NC SZC 73033 Scallion) was analyzed for the verification and accuracy of the proposed method...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738635/microwave-induced-combustion-thermal-and-morphological-aspects-for-understanding-the-mechanism-of-ignition-process-for-analytical-applications
#16
Matheus F Pedrotti, Leticia S F Pereira, Cezar A Bizzi, Jose N G Paniz, Juliano S Barin, Erico M M Flores
In the present work, for the first time a systematic study was performed using an infrared camera and scanning electron microscopy (SEM) coupled to energy dispersive X-ray spectrometry (EDS) to evaluate the mechanisms involved in microwave-induced combustion method, which has been extensively used for sample preparation. Cellulose and glass fiber discs, wetted with the igniter solution (6molL(-1) NH4NO3), were evaluated under microwave field in a monomode system. The temperature of the discs surface was recorded during microwave irradiation and the effect of NH4NO3 concentration and irradiation time on cellulose oxidation was evaluated...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738634/determination-of-synthetic-phosphodiesterase-5-inhibitors-by-lc-ms-2-in-waters-and-human-urine-submitted-to-dispersive-liquid-liquid-microextraction
#17
Natalia Campillo, Javier Marín, José Fenoll, Isabel Garrido, Ignacio López-García, Manuel Hernández-Córdoba, Pilar Viñas
High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS(2)) with a triple quadrupole is proposed for determining the synthetic phosphodiesterase-5 inhibitors, sildenafil, tadalafil and vardenafil, and the active metabolite N-desmethyl sildenafil. The method was successfully applied to the analysis of waters of different origins and human urine samples. Dispersive liquid-liquid microextraction (DLLME) was applied in the classical way for water analysis, whereas a previous extraction into an organic solvent was necessary for urine samples, the acetonitrile extract being used as dispersant solvent in the DLLME step...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738631/a-review-aptamer-based-analytical-strategies-using-the-nanomaterials-for-environmental-and-human-monitoring-of-toxic-heavy-metals
#18
REVIEW
Leila Farzin, Mojtaba Shamsipur, Shahab Sheibani
Recent developments in biotechnology offer the new methods for the sensitive detection of heavy metals based on the affinity and specificity of aptamers, as nucleic acid ligands selected from random sequence pools in vitro. Heavy metals have received considerable importance as the most toxic metallic pollutants which may cause serious environmental damages. They are classified as trace elements because of their presence in trace concentrations in various environmental matrices. Thus, the precise and sensitive methods to detect heavy metals are important to ensure human and environment safety...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738630/simultaneous-determination-of-paracetamol-and-ciprofloxacin-in-biological-fluid-samples-using-a-glassy-carbon-electrode-modified-with-graphene-oxide-and-nickel-oxide-nanoparticles
#19
Anderson Martin Santos, Ademar Wong, Adriana Araújo Almeida, Orlando Fatibello-Filho
A simple and highly selective electrochemical method using a glassy carbon electrode (GCE) modified with graphene oxide (GO) and nickel oxide nanoparticles (NiONPs) was developed for the simultaneous determination of paracetamol (PAR) and ciprofloxacin (CIP). The electrochemical characterisation of the modified GCE was performed by cyclic voltammetry and electrochemical impedance spectroscopy. The morphological characterisation of the GO and NiONPs was performed by scanning electron microscopy and transmission electron microscopy...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738629/innovative-simple-and-green-ultrasound-assisted-enzyme-based-hydrolytic-microextraction-method-for-manganese-at-trace-levels-in-food-samples
#20
Erkan Yilmaz, Mustafa Soylak
We describe the usage of hydrolytic enzymes for the innovative, green and simple microextraction of manganese in food samples. The ultrasound assisted-enzyme based hydrolytic microextraction method (UA-EH-ME) was applied for the separation and determination of manganese in food samples prior to its micro-sampling flame atomic absorption spectrometric determination (MS-FAAS). Analytical parameters influenced on the enzyme based hydrolytic microextraction method such as pH, type of enzyme, amount of enzyme, temperature and time of ultrasonic waves were fully evaluated and optimized by using mg level of NCS ZC73033 scallion certified reference material...
November 1, 2017: Talanta
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