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https://www.readbyqxmd.com/read/29034864/evaluation-of-two-commercial-real-time-pcr-assays-for-detection-of-carbapenemase-genes-in-enterobacteriaceae
#1
Liat Ashkenazi Hoffnung, Ela Burdelova, Amos Adler
The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenem-resistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100 % sensitivity and specificity...
October 16, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29033875/-i-will-survive-a-construct-validation-study-on-the-measurement-of-sustainable-employability-using-different-age-conceptualizations
#2
Pascale M Le Blanc, Beatrice I J M Van der Heijden, Tinka Van Vuuren
Though the importance of sustainable employability throughout people's working life is undisputed, up till now only one attempt for a conceptual definition has been made (van der Klink et al., 2016). Following the suggestions to further refine and improve this definition recently put forward by Fleuren et al. (2016), we propose an approach to sustainable employability that is based on the Ability-Motivation-Opportunity (AMO) framework, and incorporates three indicators: the ability, the motivation, and the opportunity to continue working, respectively...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/29033461/isotope-labeled-aspartate-sidechain-as-a-non-perturbing-infrared-probe-application-to-investigate-the-dynamics-of-a-carboxylate-buried-inside-a-protein
#3
Rachel M Abaskharon, Stephen P Brown, Wenkai Zhang, Jianxin Chen, Amos B Smith, Feng Gai
Because of their negatively charged carboxylates, aspartate and glutamate are frequently found at the active or binding site of proteins. However, studying a specific carboxylate in proteins that contain multiple aspartates and/or glutamates via infrared spectroscopy is difficult due to spectral overlap. We show, herein, that isotopic-labeling of the aspartate sidechain can overcome this limitation as the resultant (13)C=O asymmetric stretching vibration resides in a transparent region of the protein IR spectrum...
September 1, 2017: Chemical Physics Letters
https://www.readbyqxmd.com/read/29028323/benchmarking-of-computational-methods-for-creation-of-retention-models-in-quantitative-structure-retention-relationships-studies
#4
Ruth I J Amos, Eva Tyteca, Mohammad Talebi, Paul Raymond Haddad, Roman Szucs, John W Dolan, Christopher Andrew Pohl
Quantitative Structure - Retention Relationship (QSRR) models are powerful techniques for the prediction of retention times of analytes, where chromatographic retention parameters are correlated with molecular descriptors encoding chemical structures of analytes. Many QSRR models contain geometrical descriptors derived from the 3-D spatial coordinates of computationally predicted structures for the analytes. Therefore, it is sensible to calculate these structures correctly, as any error is likely to carry over to the resulting QSRR models...
October 13, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/29027735/temporal-trends-in-percutaneous-coronary-interventions-thru-the-drug-eluting-stent-era-insights-from-18-641-procedures-performed-over-12-year-period
#5
Uri Landes, Tamir Bental, Amos Levi, Abid Assali, Hana Vaknin-Assa, Eli I Lev, Eldad Rechavia, Gabriel Greenberg, Katia Orvin, Ran Kornowski
BACKGROUND: The last decade, regarded as the DES era in PCI, has witnessed significant advances in the management of coronary disease. We aimed to assess temporal trends in the practice and outcome of percutaneous coronary intervention (PCI) during the drug eluting stent (DES) era. METHODS: We analyzed 18,641 consecutive PCI's performed between January 2004 and December 2016, distinguished by procedural date (Q1 : 2004-2006, n = 4,865; Q2 : 2007-2009, n = 4,977; Q3 : 2010-2012, n = 4,230; Q4 : 2013-2016, n = 4,569)...
October 13, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29024282/anion-relay-chemistry-development-of-an-effective-diastereoselective-3-2-annulation-tactic-exploiting-an-aldol-brook-rearrangement-cyclization-cascade
#6
Heeoon Han, Amos B Smith
An effective [3+2] annulation tactic for the construction of diverse bicyclic compounds possessing highly functionalized cyclopentane rings has been developed employing soft ketone enolates as the initial nucleophile for anion relay chemistry (ARC). The protocol entails a highly diastereoselective aldol/Brook rearrangement/cyclization cascade.
October 10, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29022749/pediatric-precursor-b-cell-acute-lymphoblastic-leukemia-with-myc-8q24-translocation-how-to-treat
#7
Chuer Zhang, Gladstone Austin Amos Burke
Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer. Within ALL, precursor B-cell disease predominates and now has survival >90%. Mature B-cell, or Burkitt leukemia/lymphoma, is distinct from ALL and requires short intensive chemotherapy and with the addition of Rituximab, survival rates of >95% are achieved. Its defining characteristic is MYC translocation at 8q24. Patients who have features of both ALL and Burkitt leukemia/lymphoma represent a rare subpopulation of ALL and present a diagnostic and treatment conundrum...
October 12, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/29021774/corrigendum-marital-satisfaction-sex-age-marriage-duration-religion-number-of-children-economic-status-education-and-collectivistic-values-data-from-33-countries
#8
Piotr Sorokowski, Ashley K Randall, Agata Groyecka, Tomasz Frackowiak, Katarzyna Cantarero, Peter Hilpert, Khodabakhsh Ahmadi, Ahmad M Alghraibeh, Richmond Aryeetey, Anna Bertoni, Karim Bettache, Marta Błazejewska, Guy Bodenmann, Tiago S Bortolini, Carla Bosc, Marina Butovskaya, Felipe N Castro, Hakan Cetinkaya, Diana Cunha, Daniel David, Oana A David, Fahd A Dileym, Alejandra C Domínguez Espinosa, Silvia Donato, Daria Dronova, Seda Dural, Maryanne Fisher, Aslihan Hamamcıoğlu Akkaya, Takeshi Hamamura, Karolina Hansen, Wallisen T Hattori, Ivana Hromatko, Evrim Gülbetekin, Raffaella Iafrate, Bawo James, Feng Jiang, Charles O Kimamo, Firat Koç, Anna Krasnodębska, Amos Laar, Fívia A Lopes, Rocio Martinez, Norbert Meskó, Natalya Molodovskaya, Khadijeh Moradi Qezeli, Zahrasadat Motahari, Jean C Natividade, Joseph Ntayi, Oluyinka Ojedokun, Mohd S B Omar-Fauzee, Ike E Onyishi, Barış Özener, Anna Paluszak, Alda Portugal, Anu Realo, Ana P Relvas, Muhammad Rizwan, Agnieszka L Sabiniewicz, Svjetlana Salkicević, Ivan Sarmány-Schuller, Eftychia Stamkou, Stanislava Stoyanova, Denisa Šukolová, Nina Sutresna, Meri Tadinac, Andero Teras, Edna L T Ponciano, Ritu Tripathi, Nachiketa Tripathi, Mamta Tripathi, Maria E Yamamoto, Gyesook Yoo, Agnieszka Sorokowska
[This corrects the article on p. 1199 in vol. 8, PMID: 28785230.].
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/29021397/varicella-zoster-virus-expresses-multiple-small-non-coding-rnas
#9
Amos Markus, Linoy Golani, Nishant Kumar Ojha, Tatiana Borodiansky-Shteinberg, Paul R Kinchington, Ronald S Goldstein
Many herpesviruses express small non coding RNAs (sncRNA), including microRNAs (miR), that may play roles in regulating lytic and latent infections. None have yet been reported in varicella zoster virus (VZV, HHV-3). Here we analyzed next generation sequencing (NGS) data for small RNAs in VZV-infected fibroblasts and human embryonic stem cell-derived (hESC) neurons. Two independent bioinformatics analyses identified more than 20 VZV-encoded 20-24 nucleotide RNAs, some of which are predicted to have stem-loop precursors potentially representing miRs...
October 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29020180/molecular-tracing-of-the-geographical-origin-of-hiv-1-infection-and-patterns-of-epidemic-spread-among-migrants-who-inject-drugs-in-athens
#10
D Paraskevis, E Kostaki, G K Nikolopoulos, V Sypsa, M Psichogiou, J Del Amo, I Hodges-Mameletzis, D Paraskeva, A Skoutelis, M Malliori, L Williams, S R Friedman, G L Daikos, A Hatzakis
Background: High numbers of HIV-1 infections among people who inject drugs (PWID) have been diagnosed in Athens, Greece since 2011. We aimed to trace the geographic origin of HIV-1 infection for migrant who inject drugs and to investigate whether transmissions occur more frequently among migrants than among Greek nationals. Methods: Multiple cross-sectional studies were pooled to assemble all persons diagnosed with HIV-1 in Greece between 1/1/2011-31/10/2014. Phylogenetic analyses used maximum likelihood estimation...
August 16, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29017550/improving-family-planning-services-delivery-and-uptake-experiences-from-the-reversing-the-stall-in-fertility-decline-in-western-kenya-project
#11
Joshua Amo-Adjei, Michael Mutua, Sherine Athero, Chimaraoke Izugbara, Alex Ezeh
OBJECTIVE: In this paper, we reflect on our experiences of implementing a multipronged intervention to improve sexual and reproductive health outcomes. The project used family planning as its entry point and was implemented in two high fertility counties-Busia and Siaya in Kenya. The intervention, implemented by a seven-member consortium, involved: family planning services delivery; regular training of service providers to deliver high quality services; monitoring and evaluation; strengthening of commodity chain delivery and forecasting; school-based and out-of-school based sexuality education; and advocacy and stakeholder engagements at the community, county and national levels...
October 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28992784/an-interview-with-frances-dark-on-peer-workers-in-the-mental-health-setting
#12
Andrew Amos
No abstract text is available yet for this article.
October 2017: Australasian Psychiatry: Bulletin of Royal Australian and New Zealand College of Psychiatrists
https://www.readbyqxmd.com/read/28989077/comparison-of-efficiencies-of-selected-sample-extraction-techniques-for-the-analysis-of-selected-antiretroviral-drugs-in-human-plasma-using-lc-ms
#13
Kwenga Sichilongo, Elliott Mwando, Enoch Sepako, Amos Massele
INTRODUCTION: Sample preparation in bio analytical chemistry poses a challenge because it can be compound dependent. We compared six sample extraction techniques i.e. QuEChERS (Q), liquid extraction (LE), protein precipitation (PPT), Q-PPT, Q-LE and LE-PPT for the extraction of antiretroviral drugs emtricitabine, tenofovir, efavirenz, lopinavir and rotinavir in human blood plasma. METHOD: A multiple reaction monitoring liquid chromatography- tandem mass spectrometry method for the determination of the same antiretroviral drugs developed and validated in this laboratory was used...
October 5, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/28988422/comparison-of-two-cytoreductive-regimens-for-%C3%AE-%C3%AE-t-cell-depleted-haploidentical-hsct-in-pediatric-malignancies-improved-engraftment-and-outcome-with-tbi-based-regimen
#14
Elad Jacoby, Nira Varda-Bloom, Gal Goldstein, Daphna Hutt, Chaim Churi, Helly Vernitsky, Amos Toren, Bella Bielorai
BACKGROUND: Graft manipulation using selective depletion of αβ-T cells provides a source of haploidentical hematopoietic stem cell transplantation (haplo-HSCT) enriched in effector cells. We report our experience implementing this haplo-HSCT for high-risk malignancies in pediatric patients focusing on the conditioning regimen. PROCEDURE: We performed a retrospective study of patients who underwent T-cell receptor αβ-depleted haplo-HSCT for high-risk pediatric malignancies...
October 8, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28986515/improving-admission-medication-reconciliation-with-pharmacists-or-pharmacy-technicians-in-the-emergency-department-a-randomised-controlled-trial
#15
Joshua M Pevnick, Caroline Nguyen, Cynthia A Jackevicius, Katherine A Palmer, Rita Shane, Galen Cook-Wiens, Andre Rogatko, Mackenzie Bear, Olga Rosen, David Seki, Brian Doyle, Anish Desai, Douglas S Bell
BACKGROUND: Admission medication history (AMH) errors frequently cause medication order errors and patient harm. OBJECTIVE: To quantify AMH error reduction achieved when pharmacy staff obtain AMHs before admission medication orders (AMO) are placed. METHODS: This was a three-arm randomised controlled trial of 306 inpatients. In one intervention arm, pharmacists, and in the second intervention arm, pharmacy technicians, obtained initial AMHs prior to admission...
October 6, 2017: BMJ Quality & Safety
https://www.readbyqxmd.com/read/28986214/different-roles-of-a-novel-shrimp-microrna-in-white-spot-syndrome-virus-wssv-and-vibrio-alginolyticus-infection
#16
Zhi Wang, Fei Zhu
In this study, Marsupeneaus japonicus microRNA-S5 (miR-S5) was found to be up-regulated 24 h post white spot syndrome virus (WSSV) or V. alginolyticus infection. The loss of function using an anti-microRNA oligonucleotide (AMO-miR-S5) showed that expression levels of multiple innate immune-related genes were affected. The expression of p53 and tumor necrosis factor-α (TNF-α) were significantly down-regulated, expression of myosin was significantly up-regulated. The miR-S5 knockdown delayed WSSV-induced death for 48 h, but the final mortality was not affected, while V...
October 3, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28984493/integration-of-tuberculosis-and-hiv-services-exploring-the-perspectives-of-co-infected-patients-in-ghana
#17
Prince Justin Anku, Joshua Amo-Adjei, David Teye Doku, Akwasi Kumi-Kyereme
Scaling up of integrated tuberculosis (TB)-human immunodeficiency virus (HIV) services remains sub-optimal in many resource-limited countries, including Ghana, where the two conditions take a heavy toll on the financial resources of health systems as well as infected persons. Previous studies have documented several implementation challenges towards TB-HIV service integration, but views of patients on integrated service delivery have not received commensurate research attention. This paper explored the experiences of 40 TB-HIV co-infected patients at different stages of treatment in Ghana...
October 6, 2017: Global Public Health
https://www.readbyqxmd.com/read/28983761/brucellosis-in-migratory-sheep-flock-from-maharashtra-india
#18
Chhaya P Sonekar, Satyajit Kale, Smita Bhoyar, Neha Paliwal, S V Shinde, S P Awandkar, Wiqar Khan, S P Chaudhari, N V Kurkure
Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66...
October 6, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28982173/induced-gamma-band-activity-from-eeg-as-a-possible-index-of-training-related-brain-plasticity-in-motor-tasks
#19
Carlos Amo, Luis De Santiago, Daniel Zarza Luciáñez, José Miguel León Alonso-Cortés, Miguel Alonso-Alonso, Rafael Barea, Luciano Boquete
The aim of this study was proposing gamma band activity (GBA) as an index of training-related brain plasticity in the motor cortex. Sixteen controls underwent an experimental session where electroencephalography (EEG) activity was recorded at baseline (resting) and during a motor task (hand movements). GBA was obtained from the EEG data at baseline and during the task. Index of plasticity (IP) was defined as the relationship between GBA at the end of the motor task (GBAM_FIN), divided by GBA at the beginning of the task (GBAM_INI) for movements of both hands...
2017: PloS One
https://www.readbyqxmd.com/read/28979982/deviations-from-clinical-trials-registration-in-the-ed-safe-study
#20
Andrew James Amos
No abstract text is available yet for this article.
October 4, 2017: JAMA Psychiatry
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