Nele Beutler, Sebastian Hauka, Alexandra Niepel, Daniel Johannes Kowalewski, Julia Uhlmann, Esther Ghanem, Steffen Erkelenz, Constanze Wiek, Helmut Hanenberg, Heiner Schaal, Stefan Stevanović, Sebastian Springer, Frank Momburg, Hartmut Hengel, Anne Halenius
To assure efficient MHC class I (MHC-I) peptide loading, the peptide loading complex (PLC) recruits the peptide-receptive form of MHC-I, and in this process, tapasin (tpn) connects MHC-I with the peptide transporter TAP and forms a stable disulfide bond with ERp57. Here, we describe an alternatively spliced tpn transcript lacking exon 3, observed in cells infected with human cytomegalovirus. Recognition of exon 3 was regulated via G-runs, suggesting that members of the hnRNP (heterogeneous nuclear ribonucleoprotein)-family regulate expression of the ΔExon3 variant of tpn...
June 2013: European Journal of Immunology