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https://www.readbyqxmd.com/read/28319295/cytosine-radical-cation-a-gas-phase-study-combining-irmpd-spectroscopy-uv-pd-spectroscopy-ion-molecule-reactions-and-theoretical-calculations
#1
Michael Lesslie, John T Lawler, Andy Dang, Joseph A Korn, Daniel Bim, Vincent Steinmetz, Philippe Maitre, Frantisek Turecek, Victor Ryzhov
The radical cation of cytosine (Cyt*+) was generated via dissociative oxidation from a ternary Cu(II) complex in the gas phase. The radical cation was characterized by infrared multiple photon dissociation (IRMPD) spectroscopy in the fingerprint region, UV-VIS photodissociation (UV-PD), ion-molecule reactions, and theoretical calculations (density functional theory and ab initio). The IRMPD experimental spectrum has diagnostic bands for two enol/amino and two keto/amino tautomers of Cyt*+, that were calculated to be among the lowest-energy isomers in concert with a previous work (J...
March 20, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28319093/human-haematopoietic-stem-cell-lineage-commitment-is-a-continuous-process
#2
Lars Velten, Simon F Haas, Simon Raffel, Sandra Blaszkiewicz, Saiful Islam, Bianca P Hennig, Christoph Hirche, Christoph Lutz, Eike C Buss, Daniel Nowak, Tobias Boch, Wolf-Karsten Hofmann, Anthony D Ho, Wolfgang Huber, Andreas Trumpp, Marieke A G Essers, Lars M Steinmetz
Blood formation is believed to occur through stepwise progression of haematopoietic stem cells (HSCs) following a tree-like hierarchy of oligo-, bi- and unipotent progenitors. However, this model is based on the analysis of predefined flow-sorted cell populations. Here we integrated flow cytometric, transcriptomic and functional data at single-cell resolution to quantitatively map early differentiation of human HSCs towards lineage commitment. During homeostasis, individual HSCs gradually acquire lineage biases along multiple directions without passing through discrete hierarchically organized progenitor populations...
March 20, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28317321/development-of-a-home-based-auditory-training-to-improve-speech-recognition-on-the-telephone-for-patients-with-cochlear-implants-a-randomized-trial
#3
Friedrich Ihler, Jenny Blum, Gino Steinmetz, Bernhard G Weiss, Stefan Zirn, Martin Canis
OBJECTIVES: Speech recognition on the telephone poses a challenge for patients with cochlear implants due to a reduced bandwidth of transmission. This trial evaluates a home-based auditory training with telephone-specific filtered speech material to improve sentence recognition. DESIGN: Randomized controlled parallel double-blind. SETTING: One tertiary referral center. PARTICIPANTS: 20 postlingually deafened patients with cochlear implants...
March 20, 2017: Clinical Otolaryngology
https://www.readbyqxmd.com/read/28315832/modulating-crossover-frequency-and-interference-for-obligate-crossovers-in-saccharomyces-cerevisiae-meiosis
#4
Parijat Chakraborty, Ajith V Pankajam, Gen Lin, Abhishek Dutta, Krishnaprasad G Nandanan, Manu M Tekkedil, Akira Shinohara, Lars M Steinmetz, Nishant K Thazath
Meiotic crossover frequencies show wide variation among organisms. But most organisms maintain at least one crossover per homolog pair (obligate crossover). In Saccharomyces cerevisiae, previous studies have shown crossover frequencies are reduced in the mismatch repair related mutant mlh3Δ and enhanced in a meiotic checkpoint mutant pch2Δ by up to two-fold at specific chromosomal loci, but both mutants maintain high spore viability. We analyzed meiotic recombination events genome-wide in mlh3Δ, pch2Δ and mlh3Δ pch2Δ mutants to test the effect of variation in crossover frequency on obligate crossovers...
March 17, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28295724/improved-infrared-spectra-prediction-by-dft-from-a-new-experimental-database
#5
Madanakrishna Katari, Edith Nicol, Vincent Steinmetz, Guillaume van der Rest, Duncan Carmichael, Gilles Frison
This work aims to improve the computation of infrared spectra of gas-phase cations using DFT methods. Experimental IRMPD spectra for ten Zn and Ru organometallic complexes have been used to provide reference data for 64 vibrational modes in the 900-2000 cm-1 range. The accuracy of the IR vibrational frequencies predicted for these bands has been assessed over five DFT functionals and three basis sets. The functionals include the popular B3LYP and M06-2X hydrids and the range-separated hybrids (RSH) CAM-B3LYP, LC-BLYP and wB97X-D...
March 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28290984/kinesin-4-kif21b-is-a-potent-microtubule-pausing-factor
#6
Wilhelmina E van Riel, Ankit Rai, Sarah Bianchi, Eugene A Katrukha, Qingyang Liu, Albert Jr Heck, Casper C Hoogenraad, Michel O Steinmetz, Lukas C Kapitein, Anna Akhmanova
Microtubules are dynamic polymers that in cells can grow, shrink or pause, but the factors that promote pausing are poorly understood. Here, we show that the mammalian kinesin-4 KIF21B is a processive motor that can accumulate at microtubule plus ends and induce pausing. A few KIF21B molecules are sufficient to induce strong growth inhibition of a microtubule plus end in vitro. This property depends on non-motor microtubule-binding domains located in the stalk region and the C-terminal WD40 domain. The WD40-containing KIF21B tail displays preference for a GTP- over a GDP-type microtubule lattice and contributes to the interaction of KIF21B with microtubule plus ends...
March 14, 2017: ELife
https://www.readbyqxmd.com/read/28276440/deconvolution-of-buparlisib-s-mechanism-of-action-defines-specific-pi3k-and-tubulin-inhibitors-for-therapeutic-intervention
#7
Thomas Bohnacker, Andrea E Prota, Florent Beaufils, John E Burke, Anna Melone, Alison J Inglis, Denise Rageot, Alexander M Sele, Vladimir Cmiljanovic, Natasa Cmiljanovic, Katja Bargsten, Amol Aher, Anna Akhmanova, J Fernando Díaz, Doriano Fabbro, Marketa Zvelebil, Roger L Williams, Michel O Steinmetz, Matthias P Wymann
BKM120 (Buparlisib) is one of the most advanced phosphoinositide 3-kinase (PI3K) inhibitors for the treatment of cancer, but it interferes as an off-target effect with microtubule polymerization. Here, we developed two chemical derivatives that differ from BKM120 by only one atom. We show that these minute changes separate the dual activity of BKM120 into discrete PI3K and tubulin inhibitors. Analysis of the compounds cellular growth arrest phenotypes and microtubule dynamics suggest that the antiproliferative activity of BKM120 is mainly due to microtubule-dependent cytotoxicity rather than through inhibition of PI3K...
March 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28275573/endovascular-management-of-a-giant-symptomatic-gluteal-artery-aneurysm-with-selective-arterial-embolization
#8
Valentin Crespy, Olivier Chevallier, Joaquim Dominguez, Caroline Kazadjian, Eric Steinmetz, Pierre Pottecher, Romaric Loffroy
No abstract text is available yet for this article.
February 2017: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/28252302/influence-of-organic-chemicals-on-water-molecule-bridges-in-soil-organic-matter-of-a-sapric-histosol
#9
Pavel Ondruch, Jiri Kucerik, Zacharias Steinmetz, Gabriele E Schaumann
Water molecules in soil organic matter (SOM) can form clusters bridging neighboring molecular segments (water molecule bridges, WaMBs). WaMBs are hypothesized to enhance the physical entrapment of organic chemicals and to control the rigidity of the SOM supramolecular structure. However, the understanding of WaMBs dynamics in SOM is still limited. We investigated the relation between WaMBs stability and the physicochemical properties of their environment by treating a sapric histosol with various solvents and organic chemicals...
March 16, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28240801/longitudinal-quantitative-mri-assessment-of-cortical-damage-in-multiple-sclerosis-a-pilot-study
#10
René-Maxime Gracien, Sarah C Reitz, Stephanie-Michelle Hof, Vinzenz Fleischer, Amgad Droby, Mathias Wahl, Helmuth Steinmetz, Sergiu Groppa, Ralf Deichmann, Johannes C Klein
PURPOSE: Quantitative MRI (qMRI) allows assessing cortical pathology in multiple sclerosis (MS) on a microstructural level, where cortical damage has been shown to prolong T1 -relaxation time and increase proton density (PD) compared to controls. However, the evolution of these changes in MS over time has not been investigated so far. In this pilot study we used an advanced method for the longitudinal assessment of cortical tissue change in MS patients with qMRI in comparison to cortical atrophy, as derived from conventional MRI...
February 27, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28207984/structural-basis-of-microtubule-stabilization-by-discodermolide
#11
Andrea E Prota, Katja Bargsten, Mariano Redondo, Amos B Smith Iii, Chia-Ping H Yang, Hayley M McDaid, Ian Paterson, Susan B Horwitz, José Fernando Díaz, Michel O Steinmetz
Microtubule-stabilizing agents (MSAs) are widely used in chemotherapy. Here, using X-ray crystallography we describe the detailed binding modes of two potent MSAs, (+)-discodermolide (DDM) and the DDM-paclitaxel-hybrid KS-1-199-32, in the taxane pocket of ß-tubulin. Both compounds bind in a very similar hairpin conformation as previously observed in solution. However, they differentially stabilize the M-loop of ß-tubulin: KS-1-199-32 induces an M-loop helical conformation that is not observed for DDM. In the context of the microtubule structure, both MSAs connect the ß-tubulin helices H6 and H7 and loop S9-S10 with the M-loop, which is similar to the structural effects elicited by epothilone A, but distinct from paclitaxel...
February 16, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28199769/protein-templated-formation-of-an-inhibitor-of-the-blood-coagulation-factor%C3%A2-xa-through-a-background-free-amidation-reaction
#12
Mike Jaegle, Torsten Steinmetzer, Jörg Rademann
Protein-templated reactions enable the target-guided formation of protein ligands from reactive fragments, ideally with no background reaction. Herein, we investigate the templated formation of amides. A nucleophilic fragment that binds to the coagulation factor Xa was incubated with the protein and thirteen differentially activated dipeptides. The protein induced a non-catalytic templated reaction for the phenyl and trifluoroethyl esters; the latter was shown to be a completely background-free reaction. Starting from two fragments with millimolar affinity, a 29 nm superadditive inhibitor of factor Xa was obtained...
February 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28193641/a-method-for-high-throughput-production-of-sequence-verified-dna-libraries-and-strain-collections
#13
Justin D Smith, Ulrich Schlecht, Weihong Xu, Sundari Suresh, Joe Horecka, Michael J Proctor, Raeka S Aiyar, Richard A O Bennett, Angela Chu, Yong Fuga Li, Kevin Roy, Ronald W Davis, Lars M Steinmetz, Richard W Hyman, Sasha F Levy, Robert P St Onge
The low costs of array-synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We have developed a cost-effective method called Recombinase Directed Indexing (REDI), which involves integration of a complex library into yeast, site-specific recombination to index library DNA, and next-generation sequencing to identify desired clones...
February 13, 2017: Molecular Systems Biology
https://www.readbyqxmd.com/read/28192676/crossing-boundaries
#14
Leighton Ku, Erika Steinmetz, Tyler Bysshe, Brian K Bruen
OBJECTIVES: Previous state interagency collaborations have led to successful tobacco cessation initiatives. The objective of this study was to assess the roles and interaction of state Medicaid and public health agency efforts to support tobacco cessation for low-income Medicaid beneficiaries. METHODS: We interviewed Medicaid and state public health agency officials in 8 states in September and October 2015 about collaborations in policy development and implementation for Medicaid tobacco cessation, including Medicaid coverage policies, quitlines, and monitoring...
March 2017: Public Health Reports
https://www.readbyqxmd.com/read/28188208/quantitative-detection-of-burkholderia-pseudomallei-in-soil-samples-using-multiple-gene-targets-in-real-time-pcr
#15
A Göhler, T T Trung, V Hopf, C Kohler, J Hartleib, V Wuthiekanun, S J Peacock, D Limmathurotsakul, A Tuanyok, I Steinmetz
Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment is a prerequisite to assess the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a qPCR targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from comparative genomic analysis...
February 10, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28175308/population-pharmacokinetics-and-dosing-simulations-of-ceftazidime-in-critically-ill-patients-receiving-sustained-low-efficiency-dialysis
#16
Christina König, Stephan Braune, Jason A Roberts, Axel Nierhaus, Oliver M Steinmetz, Michael Baehr, Otto R Frey, Claudia Langebrake, Stefan Kluge
No abstract text is available yet for this article.
February 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28169959/the-impact-of-preoperative-depression-on-hospital-consumer-assessment-of-healthcare-providers-and-systems-hcahps-survey-results-in-a-lumbar-fusion-population
#17
Jay M Levin, Robert D Winkelman, Gabriel A Smith, Joseph Tanenbaum, Edward C Benzel, Thomas E Mroz, Michael P Steinmetz
STUDY DESIGN: A retrospective cohort study at a single institution. OBJECTIVE: To determine the effect of preoperative depression on Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) survey scores in a lumbar fusion population. SUMMARY OF BACKGROUND DATA: HCAHPS surveys are used to assess the quality of the patient experience, and directly influences reimbursement for hospital systems and spine surgeons nationwide. Untreated depression has been linked to worse functional outcomes in spine surgery...
February 6, 2017: Spine
https://www.readbyqxmd.com/read/28167371/updating-irradiated-graphite-disposal-project-grapa-and-the-international-decommissioning-network
#18
Anthony Wickham, Hans-Jürgen Steinmetz, Patrick O'Sullivan, Michael I Ojovan
Demonstrating competence in planning and executing the disposal of radioactive wastes is a key factor in the public perception of the nuclear power industry and must be demonstrated when making the case for new nuclear build. This work addresses the particular waste stream of irradiated graphite, mostly derived from reactor moderators and amounting to more than 250,000 tonnes world-wide. Use may be made of its unique chemical and physical properties to consider possible processing and disposal options outside the normal simple classifications and repository options for mixed low or intermediate-level wastes...
February 3, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28161814/soil-myxobacteria-as-a-potential-source-of-polyketide-peptide-substances
#19
Ivana Charousová, Heinrich Steinmetz, Juraj Medo, Soňa Javoreková, Joachim Wink
Myxobacteria, a group of antimicrobial producing bacteria, have been successfully cultured and characterized from ten soil samples collected from different parts of Slovakia. A total of 79 myxobacteria belonging to four genera (Myxococcus, Corallococcus, Sorangium, and Polyangium) were isolated based on aspects of their life cycle. Twenty-five of them were purified, fermented, and screened for antimicrobial activities against 11 test microorganisms. Results indicated that crude extracts showed more significant activities against Gram-positive than against Gram-negative bacteria or fungi...
February 4, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28161286/meg-and-eeg-demonstrate-similar-test-retest-reliability-of-the-40hz-auditory-steady-state-response
#20
Kristina T Legget, Allison K Hild, Sarah E Steinmetz, Steven T Simon, Donald C Rojas
The auditory steady-state response (ASSR) is increasingly being used as a biomarker in neuropsychiatric disorders, but research investigating the test-retest reliability of this measure is needed. We previously reported ASSR reliability, measured by electroencephalography (EEG), to 40Hz amplitude-modulated white noise and click train stimuli. The purpose of the current study was to (a) assess the reliability of the MEG-measured ASSR to 40Hz amplitude-modulated white noise and click train stimuli, and (b) compare test-retest reliability between MEG and EEG measures of ASSR, which has not previously been investigated...
April 2017: International Journal of Psychophysiology
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