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Extra cellular matrix

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https://www.readbyqxmd.com/read/29049722/a-ppar-gamma-agonist-rosiglitazone-suppresses-fibrotic-response-in-human-pterygium-fibroblasts-by-modulating-the-p38-mapk-pathway
#1
Selikem Abla Nuwormegbe, Joon Hyung Sohn, Sun Woong Kim
Purpose: Fibroblast activation may play an important role in pterygium progression. Synthetic peroxisome proliferator-activated receptor γ (PPAR-γ) ligands have been shown to be effective antifibrotic agents against transforming growth factor β1 (TGF-β1) induced fibrosis in several tissues. We aimed to investigate the antifibrotic effects of the PPAR-γ ligand rosiglitazone in pterygium fibroblasts and the underlying mechanisms. Methods: Profibrotic activation was induced by TGF-β1 in primary cultured human pterygium fibroblasts and the effect of rosiglitazone treatment on α-smooth muscle actin (α-SMA), and extra cellular matrix proteins synthesis was detected by western blotting, real-time PCR, immunostaining, and flow cytometry...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29037777/multipotency-of-skeletal-muscle-stem-cells-on-their-native-substrate-and-the-expression-of-connexin-43-during-adoption-of-adipogenic-and-osteogenic-fate
#2
Mohamed I Elashry, Manuela Heimann, Sabine Wenisch, Ketan Patel, Stefan Arnhold
Muscle regeneration is performed by resident muscle stem cells called satellite cells (SC). However they are multipotent, being able to adopt adipogenic and osteogenic fate under the correct stimuli. Since SC behavior can be regulated by the extra-cellular matrix, we examined the robustness of the myogenic programme of SC on their native substrate-the surface of a myofiber. We show that the native substrate supports myogenic differentiation judged by the expression of members of the Myogenic Determination Factor (MRF) family...
October 13, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29025978/the-anti-vascular-endothelial-growth-factor-receptor-1-monoclonal-antibody-d16f7-inhibits-glioma-growth-and-angiogenesis-in-vivo
#3
Maria Grazia Atzori, Lucio Tentori, Federica Ruffini, Claudia Ceci, Elena Bonanno, Manuel Scimeca, Pedro Miguel Lacal, Grazia Graziani
The vascular endothelial growth factor receptor-1 (VEGFR-1) is a tyrosine kinase receptor that does not play a relevant role in physiological angiogenesis in adults, while it is important in tumor angiogenesis. In high-grade glioma VEGFR-1 expression by tumor endothelium and neoplastic cells contributes to the aggressive phenotype. We recently generated an anti-VEGFR-1 monoclonal antibody (mAb) characterized by a novel mechanism of action, since it hampers receptor activation without interfering with ligand binding...
October 12, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29016873/cgnl1-an-endothelial-junction-complex-protein-regulates-gtpase-mediated-angiogenesis
#4
Ihsan Chrifi, Dorien Hermkens, Maarten M Brandt, Christian G M van Dijk, Petra E Bürgisser, Remco Haasdijk, Jiayi Pei, Esther H M van de Kamp, Changbin Zhu, Lau Blonden, Johan M Kros, Dirk J Duncker, Henricus J Duckers, Caroline Cheng
Aims: The formation of cell-cell and cell-extra cellular matrix contacts by endothelial cells is crucial for the stability and integrity of a vascular network. We previously identified cingulin-like 1 (Cgnl1) in a transcriptomic screen for new angiogenic modulators. Here we aim to study the function of the cell-cell junction associated protein Cgnl1 during vessel formation. Methods and Results: Unlike family member cingulin, Cgnl1 expression is enriched in endothelial cells during vascular growth...
August 31, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28977999/platelet-derived-growth-factor-c-promotes-human-melanoma-aggressiveness-through-activation-of-neuropilin-1
#5
Federica Ruffini, Lauretta Levati, Grazia Graziani, Simona Caporali, Maria Grazia Atzori, Stefania D'Atri, Pedro M Lacal
Despite recent progress in advanced melanoma therapy, identification of signalling pathways involved in melanoma switch from proliferative to invasive states is still crucial to uncover new therapeutic targets for improving the outcome of metastatic disease. Neuropilin-1 (NRP-1), a co-receptor for vascular endothelial growth factor-A (VEGF-A) tyrosine kinase receptors (VEGFRs), has been suggested to play a relevant role in melanoma progression. NRP-1 can be activated by VEGF-A also in the absence of VEGFRs, triggering specific signal transduction pathways (e...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28975895/pla-short-i-sub-micron-fibers-i-reinforcement-of-3d-bioprinted-alginate-constructs-for-cartilage-regeneration
#6
Alicja Kosik-Kozioł, Marco Costantini, Tomasz Bolek, Krisztina Szoke, Andrea Barbetta, Jan E Brinchmann, Wojciech Święszkowski
In this study, we present an innovative strategy to reinforce 3D printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28964839/in-silico-screening-and-experimental-validation-reveal-l-adrenaline-as-anti-biofilm-molecule-against-biofilm-associated-protein-bap-producing-acinetobacter-baumannii
#7
Vishvanath Tiwari, Varsha Patel, Monalisa Tiwari
Acinetobacter baumannii, an ESKAPE pathogen, causes various nosocomial infections and has capacity to produce biofilm. Biofilm produced by this bacterium is highly tolerant to environmental factors and different antibiotics. Biofilm-associated protein (Bap) plays a significant role in the biofilm formation by A. baumannii and found in the extra cellular matrix of the biofilm. Therefore, it becomes essential to find a potential drug against Bap that has capacity to inhibit biofilm formation by A. baumannii. In-silico screening, molecular mechanics and molecular dynamics studies identified ZINC00039089 (L-Adrenaline) as an inhibitor for Bap of A...
September 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28952483/iron-oxide-nanoparticles-stimulates-extra-cellular-matrix-production-in-cellular-spheroids
#8
Megan Casco, Timothy Olsen, Austin Herbst, Grace Evans, Taylor Rothermel, Lauren Pruett, Dan Simionescu, Richard Visconti, Frank Alexis
Nanotechnologies have been integrated into drug delivery, and non-invasive imaging applications, into nanostructured scaffolds for the manipulation of cells. The objective of this work was to determine how the physico-chemical properties of magnetic nanoparticles (MNPs) and their spatial distribution into cellular spheroids stimulated cells to produce an extracellular matrix (ECM). The MNP concentration (0.03 mg/mL, 0.1 mg/mL and 0.3 mg/mL), type (magnetoferritin), shape (nanorod-85 nm × 425 nm) and incorporation method were studied to determine each of their effects on the specific stimulation of four ECM proteins (collagen I, collagen IV, elastin and fibronectin) in primary rat aortic smooth muscle cell...
January 21, 2017: Bioengineering
https://www.readbyqxmd.com/read/28943527/crossing-bridges-between-extra-and-intra-cellular-events-in-thoracic-aortic-aneurysms
#9
Yoshito Yamashiro, Hiromi Yanagisawa
Thoracic aortic aneurysms (TAAs) are common, life-threatening diseases and are a major cause of mortality and morbidity. Over the past decade, genetic approaches have revealed that 1) activation of the transforming growth factor beta (TGF-β) signaling, 2) alterations in the contractile apparatus of vascular smooth muscle cells (SMCs), and 3) defects in the extracellular matrix (ECM) were responsible for development of TAAs. Most recently, a fourth mechanism has been proposed in that dysfunction of mechanosensing in the aortic wall in response to hemodynamic stress may be a key driver of TAAs...
September 22, 2017: Journal of Atherosclerosis and Thrombosis
https://www.readbyqxmd.com/read/28901502/harmine-suppresses-the-proliferation-and-migration-of-human-ovarian-cancer-cells-through-inhibiting-erk-creb-pathway
#10
Jun Gao, Hong Zhu, Hong Wan, Xia Zou, Xiaoxin Ma, Guolan Gao
Ovarian cancer is the most lethal gynaecological cancer and the sixth most common cause of cancer related death among Western women. Recent studies show that harmine, a small-molecular β-carboline alkaloid present in medicinal plants, displayed obvious anticancer effects in several cancer cells. However, the effect of harmine on ovarian cancer is not well understood. In the present study, the effect of harmine on the cell proliferation and migration of ovarian cancer SKOV-3 cells and the underlying mechanism were investigated...
November 2017: Oncology Reports
https://www.readbyqxmd.com/read/28901271/the-role-of-cannabinoid-receptors-in-renal-diseases
#11
Hélène François, Lola Lecru
Chronic kidney disease (CKD) remains a major challenge for Public Health systems and corresponds to the replacement of renal functional tissue by extra-cellular matrix proteins such as collagens and fibronectin. There is no efficient treatment to date for CKD except nephroprotective strategies. The cannabinoid system and more specifically the cannabinoid receptors 1 (CB1) and 2 (CB2) may represent a new therapeutic target in CKD. Our review will first focus on the current state of knowledge regarding the cannabinoid system in normal renal physiology and in various experimental nephropathies, especially diabetes...
September 11, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28895559/sustained-releasing-sponge-like-3d-scaffolds-for-bone-tissue-engineering-applications
#12
Vidya Chamundeswari Narasimhan, Yuan Siang Lui, Yon Jin Chuah, Jing Shi Elaine Tan, Dong An Wang, Say Chye Joachim Loo
Tissue engineering (TE) is envisaged to play a vital role in improving the quality of life by restoring, maintaining or enhancing tissue and organ functions. TE scaffolds that are two dimensional (2D) in structure suffer from undesirable issues, such as pore blockage, and does not close mimic the native extra-cellular matrix (ECM) in tissues. Significant efforts have therefore been channeled to fabricate three-dimensional (3D) scaffolds using various techniques, especially electrospinning. In this study, we propose a modified one-step electrospinning process to arrive at a 3D scaffolds with highly interconnected pores...
September 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28888475/promoter-methylation-status-of-the-timp2-and-adamts4-genes-and-patellar-tendinopathy
#13
Louis Y El Khoury, Rebecca Rickaby, Tom Samiric, Stuart M Raleigh
OBJECTIVES: Patellar tendinopathy (PT) is a debilitating and prevalent condition that tends to affect those who are physically active or engaged in jumping sports. Although tendinopathies are known to have a genetic basis, the role of DNA methylation as an epigenetic factor and risk determinant for human PT has never been described. We sought to determine whether differences existed between the methylation profiles of both the TIMP2 and ADAMTS4 gene promoter sequences in a cohort of males having undergone surgery for patellar tendinopathy compared to controls...
September 1, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28874803/tumor-cells-and-their-crosstalk-with-endothelial-cells-in-3d-spheroids
#14
Hila Shoval, Adi Karsch-Bluman, Yifat Brill-Karniely, Tal Stern, Gideon Zamir, Ayala Hubert, Ofra Benny
Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that  exist in the original tumor. Along with this effort, 3-dimentional (3D) cellular models are being extensively studied. Spheroids are self-assembled cell aggregates that possess many important components of the physiological spatial growth and cell-cell interactions...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28859094/epithelial-heparan-sulfate-regulates-sonic-hedgehog-signaling-in-lung-development
#15
Hua He, Meina Huang, Shenfei Sun, Yihui Wu, Xinhua Lin
The tree-like structure of the mammalian lung is generated from branching morphogenesis, a reiterative process that is precisely regulated by numerous factors. How the cell surface and extra cellular matrix (ECM) molecules regulate this process is still poorly understood. Herein, we show that epithelial deletion of Heparan Sulfate (HS) synthetase Ext1 resulted in expanded branching tips and reduced branching number, associated with several mesenchymal developmental defects. We further demonstrate an expanded Fgf10 expression and increased FGF signaling activity in Ext1 mutant lungs, suggesting a cell non-autonomous mechanism...
August 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28852920/the-significance-of-aryl-acylamidase-activity-of-acetylcholinesterase-in-osteoblast-differentiation-and-mineralization
#16
Raj Kumar Chinnadurai, Ponne Saravanaraman, Rathanam Boopathy
Osteoblast differentiation is an essential event in the developmental process, which is favoured by the production of extra cellular matrix proteins and various enzymes including discrete ones like acetylcholinesterase (AChE). Despite the fact that AChE facilitates osteoblast differentiation, the significance of its catalytic functions [esterase and aryl acylamidase (AAA) activities] in the process is yet to be ascertained. In this context, SaOS-2 cell line was used in the present study to implicate the catalytic activities of AChE in process of osteoblast differentiation and mineralization...
August 29, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28846161/different-properties-of-skin-of-different-body-sites-the-root-of-keloid-formation
#17
Liselotte Butzelaar, Frank B Niessen, Wendy Talhout, Dennis Pm Schooneman, Magda M Ulrich, Robert Hj Beelen, Aebele B Mink van der Molen
The purpose of this study was to examine extra cellular matrix composition, vascularization and immune cell population of skin sites prone to keloid formation. Keloids remain a complex problem, posing esthetical as well as functional difficulties for those affected. These scars tend to develop at anatomic sites of preference. Mechanical properties of skin vary with anatomic location and depend largely on extra cellular matrix composition. These differences in extra cellular matrix composition, but also vascularization and resident immune cell populations might play a role in the mechanism of keloid formation...
August 28, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28816035/surface-modification-with-ecm-inspired-sdf-1%C3%AE-laminin-loaded-nanocoating-for-vascular-wound-healing
#18
Tao Liu, Xin Wang, Xiaohan Tang, Tao Gong, Wei Ye, Changjiang Pan, Hongyan Ding, Xun Luo, Xia Li, Qing Mei Wang
Surface biomimetic modification with extra-cellular matrix (ECM)-derived biomolecules is an emerging potential method of accelerating the healing of vascular stent lesions. However, insufficient capacity of the constructed biofunctional layer in maintaining its long-term efficiency and preventing thrombus and neointimal hyperplasia continue to be major limitations in clinical application. On the basis of the structure and function of ECM, in this study, we constructed a novel stromal cell-derived factor-1α (SDF-1α)/laminin-loaded nanocoating on the 316L stainless steel (SS) surface to provide improved function in modulation of vascular remodeling...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28798693/phosphorylation-modulates-ameloblastin-self-assembly-and-ca-2-binding
#19
Øystein Stakkestad, Ståle P Lyngstadaas, Bernd Thiede, Jiri Vondrasek, Bjørn S Skålhegg, Janne E Reseland
Ameloblastin (AMBN), an important component of the self-assembled enamel extra cellular matrix, contains several in silico predicted phosphorylation sites. However, to what extent these sites actually are phosphorylated and the possible effects of such post-translational modifications are still largely unknown. Here we report on in vitro experiments aimed at investigating what sites in AMBN are phosphorylated by casein kinase 2 (CK2) and protein kinase A (PKA) and the impact such phosphorylation has on self-assembly and calcium binding...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28782585/weaving-for-heart-valve-tissue-engineering
#20
REVIEW
Albert Liberski, Nadia Ayad, Dorota Wojciechowska, Radoslaw Kot, Duy M P Vo, Dilibaier Aibibu, Gerald Hoffmann, Chokri Cherif, Katharina Grobelny-Mayer, Marek Snycerski, Helmut Goldmann
Weaving is a resourceful technology which offers a large selection of solutions that are readily adaptable for tissue engineering (TE) of artificial heart valves (HV). The different ways that the yarns are interlaced in this technique could be used to produce complex architectures, such as the three-layer architecture of the leaflets. Once the assembly is complete, growth of cells in the scaffold would occur in the orientation of the yarn, enabling the deposition of extra cellular matrixes proteins in an oriented manner...
August 4, 2017: Biotechnology Advances
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