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Extra cellular matrix

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https://www.readbyqxmd.com/read/28231262/oleanolic-acid-induces-migration-in-mv1lu-and-mda-mb-231-epithelial-cells-involving-egf-receptor-and-map-kinases-activation
#1
Ángel Bernabé-García, David Armero-Barranco, Sergio Liarte, María Ruzafa-Martínez, Antonio Jesús Ramos-Morcillo, Francisco José Nicolás
During wound healing, skin function is restored by the action of several cell types that undergo differentiation, migration, proliferation and/or apoptosis. These dynamics are tightly regulated by the evolution of the extra cellular matrix (ECM) contents along the process. Pharmacologically active flavonoids have shown to exhibit useful physiological properties interesting in pathological states. Among them, oleanolic acid (OA), a pentacyclic triterpene, shows promising properties over wound healing, as increased cell migration in vitro and improved wound resolution in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28228548/contact-guidance-requires-spatial-control-of-leading-edge-protrusion
#2
G R Ramirez-San Juan, P W Oakes, M L Gardel
In vivo, geometric cues from the extra-cellular matrix (ECM) are critical for the regulation of cell shape, adhesion and migration. During contact guidance, the fibrillar architecture of the ECM promotes an elongated cell shape and migration along the fibrils. The subcellular mechanisms by which cells sense ECM geometry and translate it into changes in shape and migration direction, are not understood. Here, we pattern linear fibronectin features to mimic fibrillar ECM and elucidate the mechanisms of contact guidance...
February 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28227439/three-dimensional-graphene-scaffold-for-cardiac-tissue-engineering-and-in-situ-electrical-recording
#3
S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, L Black, P K Singh, W Stoppel, S R Sonkusale, R D'Angelo, S K Ameri
In this paper, we present a three-dimensional graphene foam made of few layers of CVD grown graphene as a scaffold for growing cardiac cells and recording their electrical activity. Our results show that graphene foam not only provides an excellent extra-cellular matrix (ECM) for the culture of such electrogenic cells but also enables recording of its extracellular electrical activity in-situ. Recording is possible due to graphene's excellent conductivity. In this paper, we present our results on the fabrication of the graphene scaffold and initial studies on the culture of cardiac cell lines such as HL-1 and recording of their real-time electrical activity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28221758/synthesis-and-fabrication-of-collagen-coated-ostholamide-electrospun-nanofiber-scaffold-for-wound-healing
#4
Subramani Kandhasamy, Sathiamurthi Perumal, Balaraman Madhan, Narayanan Umamaheswari, Javaid Ahmad Banday, Paramasivan Thirumalai Perumal, Vichangal Pridiuldi Santhanakrishnan
A novel scaffold for effective wound healing treatment was developed utilizing natural product bearing collagen based biocompatible electro-spun nanofibres. Initially, ostholamide (OSA) was synthesized from osthole (a natural coumarin), characterized by 1H, 13C, DEPT-135 NMR, ESI-MS and FT-IR spectroscopy analysis. OSA was incorporated into Polyhydroxybutyrate (PHB) and Gelatin (GEL), which serve as templates for electro-spun nanofibers. The coating of OSA-PHB-GEL nanofibers with collagen resulted in PHB-GEL-OSA-COL nanofibrous scaffold which mimics extra cellular matrix and serves as an effective biomaterial for tissue engineering applications, especially for wound healing...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28202940/mitochondrial-behaviour-throughout-the-lytic-cycle-of-toxoplasma-gondii
#5
Jana Ovciarikova, Leandro Lemgruber, Krista L Stilger, William J Sullivan, Lilach Sheiner
Mitochondria distribution in cells controls cellular physiology in health and disease. Here we describe the mitochondrial morphology and positioning found in the different stages of the lytic cycle of the eukaryotic single-cell parasite Toxoplasma gondii. The lytic cycle, driven by the tachyzoite life stage, is responsible for acute toxoplasmosis. It is known that whilst inside a host cell the tachyzoite maintains its single mitochondrion at its periphery. We found that upon parasite transition from the host cell to the extracellular matrix, mitochondrion morphology radically changes, resulting in a reduction in peripheral proximity...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28192301/dynamic-interaction-between-actin-and-nesprin2-maintain-the-cell-nucleus-in-a-prestressed-state
#6
Abhishek Kumar, G V Shivashankar
Mechanical coupling between the nucleus and the cytoskeleton is indispensable for direct force transduction from the extra cellular matrix (ECM) to the chromatin. Although this physical coupling has been shown to be crucial for nuclear positioning and its function, the quantification of nuclear-cytoskeleton interaction has been lacking. In this paper, using various quantitative fluorescence spectroscopy techniques, we investigate the nature of this connection. High-resolution 3D imaging shows that nesprin2G forms short linear structures along actin stress fibers (ASFs) in the apical region of the nucleus...
November 11, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28190551/proliferation-of-canine-bone-marrow-derived-mesenchymal-stem-cells-on-different-nanomaterial-based-thin-film-scaffolds
#7
Kinsuk Das, Bhabesh Mili, Madhusoodan A P, Abhishek Chandra Saxena, Ajay Kumar, Praveen Singh, Med Ram Verma, Mihir Sarkar, Sadhan Bag
Stem cell niche research uses nanotechnologies to mimic the extra-cellular microenvironment to promote proliferation and differentiation. The aim of designing different scaffolds is to simulate the best structural and environmental pattern for extracellular matrix. This experiment was designed to study the proliferative behaviour of canine bone marrow deriver mesenchymal stem cells (MSCs) on different nanomaterial based thin film scaffolds of carbon nanotubes (CNT), chitosan and poly ε-caprolactone. Similar number of cells was seeded on the scaffolds and standard cell culture flask, taken as control...
February 6, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28183649/biological-and-mechanical-evaluation-of-a-bio-hybrid-scaffold-for-autologous-valve-tissue-engineering
#8
S Jahnavi, U Saravanan, N Arthi, G S Bhuvaneshwar, T V Kumary, S Rajan, R S Verma
Major challenge in heart valve tissue engineering for paediatric patients is the development of an autologous valve with regenerative capacity. Hybrid tissue engineering approach is recently gaining popularity to design scaffolds with desired biological and mechanical properties that can remodel post implantation. In this study, we fabricated aligned nanofibrous Bio-Hybrid scaffold made of decellularized bovine pericardium: polycaprolactone-chitosan with optimized polymer thickness to yield the desired biological and mechanical properties...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28179886/does-oxidative-stress-induced-by-alcohol-consumption-affect-orthodontic-treatment-outcome
#9
REVIEW
Jorge M Barcia, Sandra Portolés, Laura Portolés, Alba C Urdaneta, Verónica Ausina, Gema M A Pérez-Pastor, Francisco J Romero, Vincent M Villar
HIGHLIGHTS Ethanol, Periodontal ligament, Extracellular matrix, Orthodontic movement. Alcohol is a legal drug present in several drinks commonly used worldwide (chemically known as ethyl alcohol or ethanol). Alcohol consumption is associated with several disease conditions, ranging from mental disorders to organic alterations. One of the most deleterious effects of ethanol metabolism is related to oxidative stress. This promotes cellular alterations associated with inflammatory processes that eventually lead to cell death or cell cycle arrest, among others...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28125550/o-002-predicting-a-complicated-course-for-children-newly-diagnosed-with-crohn-s-disease-a-multicenter-inception-cohort-study
#10
Subra Kugathasan, Lee Denson, Thomas Walters, Mi-Ok Kim, Urko Marigorta, Melanie Schirmer, Kajari Mondal, Chunyan Liu, Michael Stephens, Robert Baldassano, James Markowitz, Ramnik Xavier, Bruce Aronow, Greg Gibson, Jeffrey Hyams, Marla Dubinsky
BACKGROUND: A subset of patients with Crohn's disease (CD) rapidly progress to a stricturing or internal penetrating phenotype that requires surgery. Despite great strides in therapy, there have not been population-level declines in the rates of these serious complications or need for surgery in CD. Effective risk stratification models to predict progression to such complications in pediatric CD are presently lacking. We aimed to develop and test a prognostic model incorporating clinical and host biology and integrated the comparative effectiveness of anti-TNF in preventing stricturing or perforating complications...
February 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28122819/method-to-study-cell-migration-under-uniaxial-compression
#11
Nishit Srivastava, Robert R Kay, Alexandre J Kabla
The chemical, physical and mechanical properties of the extra-cellular environment have a strong effect on cell migration. Aspects such as pore-size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including pseudopod or blebbing. How a cell perceives its environment, and how such a cue triggers a change in behaviour are largely unknown, but mechanics is likely to be involved. Because mechanical conditions are often controlled by modifying the composition of the environment, separating chemical and physical contributions is difficult and requires multiple controls...
January 25, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#12
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28090760/expression-patterns-of-cardiac-aging-in-drosophila
#13
Leah Cannon, Alexander C Zambon, Anthony Cammarato, Zhi Zhang, Georg Vogler, Matthew Munoz, Erika Taylor, Jérôme Cartry, Sanford I Bernstein, Simon Melov, Rolf Bodmer
Aging causes cardiac dysfunction, often leading to heart failure and death. The molecular basis of age-associated changes in cardiac structure and function is largely unknown. The fruit fly, Drosophila melanogaster, is well-suited to investigate the genetics of cardiac aging. Flies age rapidly over the course of weeks, benefit from many tools to easily manipulate their genome, and their heart has significant genetic and phenotypic similarities to the human heart. Here, we performed a cardiac-specific gene expression study on aging Drosophila and carried out a comparative meta-analysis with published rodent data...
February 2017: Aging Cell
https://www.readbyqxmd.com/read/28087981/humanized-chondroitinase-abc-sensitizes-glioblastoma-cells-to-temozolomide
#14
Alena Cristina Jaime-Ramirez, Nina Dmitrieva, Ji Young Yoo, Yeshavanth Banasavadi-Siddegowda, Jianying Zhang, Theresa Relation, Chelsea Bolyard-Blessing, Jeffrey Wojton, Balveen Kaur
INTRODUCTION: Malignant gliomas (GBMs) are extremely aggressive and have a median survival of approximately 15 months. Current treatment modalities, which include surgical resection, radiation and chemotherapy, have done little to prolong the lives of GBM patients. Chondroitin sulfate proteoglycans (CSPG) are critical for cell-cell and cell-extra cellular matrix (ECM) interactions and are implicated in glioma growth and invasion. Chondroitinase (Chase) ABC is a bacterial enzyme that cleaves chondroitin sulfate disaccharide chains from CSPGs in the tumor ECM...
January 14, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28087487/bioprinting-for-vascular-and-vascularized-tissue-biofabrication
#15
REVIEW
Pallab Datta, Bugra Ayan, Ibrahim T Ozbolat
: Bioprinting is a promising technology to fabricate design-specific tissue constructs due to its ability to create complex, heterocellular structures with anatomical precision. Bioprinting enables the deposition of various biologics including growth factors, cells, genes, neo-tissues and extra-cellular matrix-like hydrogels. Benefits of bioprinting have started to make a mark in the fields of tissue engineering, regenerative medicine and pharmaceutics. Specifically, in the field of tissue engineering, the creation of vascularized tissue constructs has remained a principal challenge till date...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087448/fabrication-and-characterization-of-electrospun-cellulose-nano-hydroxyapatite-nanofibers-for-bone-tissue-engineering
#16
Chenghong Ao, Yan Niu, Ximu Zhang, Xu He, Wei Zhang, Canhui Lu
Nanofibrous scaffolds from cotton cellulose and nano-hydroxyapatite (nano-HA) were electrospun for bone tissue engineering. The solution properties of cellulose/nano-HA spinning dopes and their associated electrospinnability were characterized. Morphological, thermal and mechanical properties of the electrospun cellulose/nano-HA nanocomposite nanofibers (ECHNN) were measured and the biocompatibility of ECHNN with human dental follicle cells (HDFCs) was evaluated. Scanning electron microscope (SEM) images indicated that the average diameter of ECHNN increased with a higher nano-HA loading and the fiber diameter distributions were well within the range of natural ECM (extra cellular matrix) fibers (50-500nm)...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28043893/a-novel-microstructural-interpretation-for-the-biomechanics-of-mouse-skin-derived-from-multiscale-characterization
#17
Barbara Lynch, Stéphane Bancelin, Christelle Bonod-Bidaud, Jean-Baptiste Gueusquin, Florence Ruggiero, Marie-Claire Schanne-Klein, Jean-Marc Allain
: Skin is a complex, multi-layered organ, with important functions in the protection of the body. The dermis provides structural support to the epidermal barrier, and thus has attracted a large number of mechanical studies. As the dermis is made of a mixture of stiff fibres embedded in a soft non-fibrillar matrix, it is classically considered that its mechanical response is based on an initial alignment of the fibres, followed by the stretching of the aligned fibres. Using a recently developed set-up combining multiphoton microscopy with mechanical assay, we imaged the fibres network evolution during dermis stretching...
December 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27999805/efficiency-of-human-epiphyseal-chondrocytes-with-differential-replication-numbers-for-cellular-therapy-products
#18
Michiyo Nasu, Shinichiro Takayama, Akihiro Umezawa
The cell-based therapy for cartilage or bone requires a large number of cells; serial passages of chondrocytes are, therefore, needed. However, fates of expanded chondrocytes from extra fingers remain unclarified. The chondrocytes from human epiphyses morphologically changed from small polygonal cells to bipolar elongated spindle cells and to large polygonal cells with degeneration at early passages. Gene of type II collagen was expressed in the cells only at a primary culture (Passage 0) and Passage 1 (P1) cells...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27993655/chitosan-based-nanofibers-in-bone-tissue-engineering
#19
REVIEW
K Balagangadharan, S Dhivya, N Selvamurugan
Bone tissue engineering involves biomaterials, cells and regulatory factors to make biosynthetic bone grafts with efficient mineralization for regeneration of fractured or damaged bones. Out of all the techniques available for scaffold preparation, electrospinning is given priority as it can fabricate nanostructures. Also, electrospun nanofibers possess unique properties such as the high surface area to volume ratio, porosity, stability, permeability and morphological similarity to that of extra cellular matrix...
December 18, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27982511/biochemical-changes-in-the-niche-following-tumor-cell-invasion
#20
A M Decker, F C Cackowski, Y Jung, R S Taichman
Metastatic cancer is the leading cause of all cancer related deaths. Prostate cancer (PCa) metastasizes preferentially to the bone marrow, specifically within the endosteal niche. Endosteal cells secrete homing molecules that may recruit PCa cells to the bone marrow. Once there, the biochemical signature of this niche regulates PCa fate including cellular dormancy or cell cycle arrest, reactivation and resistance to chemotherapeutics. Growth factors, interleukins, adhesion molecules, as well as extra-cellular matrix proteins can collectively change the phenotype of PCa cells...
December 16, 2016: Journal of Cellular Biochemistry
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