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https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#1
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28090760/expression-patterns-of-cardiac-aging-in-drosophila
#2
Leah Cannon, Alexander C Zambon, Anthony Cammarato, Zhi Zhang, Georg Vogler, Matthew Munoz, Erika Taylor, Jérôme Cartry, Sanford I Bernstein, Simon Melov, Rolf Bodmer
Aging causes cardiac dysfunction, often leading to heart failure and death. The molecular basis of age-associated changes in cardiac structure and function is largely unknown. The fruit fly, Drosophila melanogaster, is well-suited to investigate the genetics of cardiac aging. Flies age rapidly over the course of weeks, benefit from many tools to easily manipulate their genome, and their heart has significant genetic and phenotypic similarities to the human heart. Here, we performed a cardiac-specific gene expression study on aging Drosophila and carried out a comparative meta-analysis with published rodent data...
February 2017: Aging Cell
https://www.readbyqxmd.com/read/28087981/humanized-chondroitinase-abc-sensitizes-glioblastoma-cells-to-temozolomide
#3
Alena Cristina Jaime-Ramirez, Nina Dmitrieva, Ji Young Yoo, Yeshavanth Banasavadi-Siddegowda, Jianying Zhang, Theresa Relation, Chelsea Bolyard-Blessing, Jeffrey Wojton, Balveen Kaur
INTRODUCTION: Malignant gliomas (GBMs) are extremely aggressive and have a median survival of approximately 15 months. Current treatment modalities, which include surgical resection, radiation and chemotherapy, have done little to prolong the lives of GBM patients. Chondroitin sulfate proteoglycans (CSPG) are critical for cell-cell and cell-extra cellular matrix (ECM) interactions and are implicated in glioma growth and invasion. Chondroitinase (Chase) ABC is a bacterial enzyme that cleaves chondroitin sulfate disaccharide chains from CSPGs in the tumor ECM...
January 14, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28087487/bioprinting-for-vascular-and-vascularized-tissue-biofabrication
#4
REVIEW
Pallab Datta, Bugra Ayan, Ibrahim T Ozbolat
: Bioprinting is a promising technology to fabricate design-specific tissue constructs due to its ability to create complex, heterocellular structures with anatomical precision. Bioprinting enables the deposition of various biologics including growth factors, cells, genes, neo-tissues and extra-cellular matrix-like hydrogels. Benefits of bioprinting have started to make a mark in the fields of tissue engineering, regenerative medicine and pharmaceutics. Specifically, in the field of tissue engineering, the creation of vascularized tissue constructs has remained a principal challenge till date...
January 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087448/fabrication-and-characterization-of-electrospun-cellulose-nano-hydroxyapatite-nanofibers-for-bone-tissue-engineering
#5
Chenghong Ao, Yan Niu, Ximu Zhang, Xu He, Wei Zhang, Canhui Lu
Nanofibrous scaffolds from cotton cellulose and nano-hydroxyapatite (nano-HA) were electrospun for bone tissue engineering. The solution properties of cellulose/nano-HA spinning dopes and their associated electrospinnability were characterized. Morphological, thermal and mechanical properties of the electrospun cellulose/nano-HA nanocomposite nanofibers (ECHNN) were measured and the biocompatibility of ECHNN with human dental follicle cells (HDFCs) was evaluated. Scanning electron microscope (SEM) images indicated that the average diameter of ECHNN increased with a higher nano-HA loading and the fiber diameter distributions were well within the range of natural ECM (extra cellular matrix) fibers (50-500nm)...
January 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28043893/a-novel-microstructural-interpretation-for-the-biomechanics-of-mouse-skin-derived-from-multiscale-characterization
#6
Barbara Lynch, Stéphane Bancelin, Christelle Bonod-Bidaud, Jean-Baptiste Gueusquin, Florence Ruggiero, Marie-Claire Schanne-Klein, Jean-Marc Allain
: Skin is a complex, multi-layered organ, with important functions in the protection of the body. The dermis provides structural support to the epidermal barrier, and thus has attracted a large number of mechanical studies. As the dermis is made of a mixture of stiff fibres embedded in a soft non-fibrillar matrix, it is classically considered that its mechanical response is based on an initial alignment of the fibres, followed by the stretching of the aligned fibres. Using a recently developed setup combining multiphoton microscopy with mechanical assay, we imaged the fibres network evolution during dermis stretching...
December 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27999805/efficiency-of-human-epiphyseal-chondrocytes-with-differential-replication-numbers-for-cellular-therapy-products
#7
Michiyo Nasu, Shinichiro Takayama, Akihiro Umezawa
The cell-based therapy for cartilage or bone requires a large number of cells; serial passages of chondrocytes are, therefore, needed. However, fates of expanded chondrocytes from extra fingers remain unclarified. The chondrocytes from human epiphyses morphologically changed from small polygonal cells to bipolar elongated spindle cells and to large polygonal cells with degeneration at early passages. Gene of type II collagen was expressed in the cells only at a primary culture (Passage 0) and Passage 1 (P1) cells...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27993655/chitosan-based-nanofibers-in-bone-tissue-engineering
#8
REVIEW
K Balagangadharan, S Dhivya, N Selvamurugan
Bone tissue engineering involves biomaterials, cells and regulatory factors to make biosynthetic bone grafts with efficient mineralization for regeneration of fractured or damaged bones. Out of all the techniques available for scaffold preparation, electrospinning is given priority as it can fabricate nanostructures. Also, electrospun nanofibers possess unique properties such as the high surface area to volume ratio, porosity, stability, permeability and morphological similarity to that of extra cellular matrix...
December 18, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27982511/biochemical-changes-in-the-niche-following-tumor-cell-invasion
#9
A M Decker, F C Cackowski, Y Jung, R S Taichman
Metastatic cancer is the leading cause of all cancer related deaths. Prostate cancer (PCa) metastasizes preferentially to the bone marrow, specifically within the endosteal niche. Endosteal cells secrete homing molecules that may recruit PCa cells to the bone marrow. Once there, the biochemical signature of this niche regulates PCa fate including cellular dormancy or cell cycle arrest, reactivation and resistance to chemotherapeutics. Growth factors, interleukins, adhesion molecules, as well as extra-cellular matrix proteins can collectively change the phenotype of PCa cells...
December 16, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27967204/mmp-1-activation-contributes-to-airway-smooth-muscle-growth-and-asthma-severity
#10
Shams-Un-Nisa Naveed, Debbie Clements, David J Jackson, Christopher Philp, Charlotte K Billington, Irshad Soomro, Catherine Reynolds, Timothy W Harrison, Sebastian L Johnston, Dominick E Shaw, Simon R Johnson
INTRODUCTION: Matrix metalloproteinase-1 and mast cells are present in the airways of people with asthma. We hypothesised that matrix metalloproteinase-1 could be activated by mast cells and increase asthma severity. METHODS: Patients with stable asthma and healthy controls underwent spirometry, methacholine challenge, bronchoscopy and their airway smooth muscle cells were grown in culture. A second asthma group and controls had symptom scores, spirometry and bronchoalveolar lavage before and after rhinovirus-induced asthma exacerbations...
December 14, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27940195/differential-integrin-expression-regulates-cell-sensing-of-the-matrix-nanoscale-geometry
#11
Stefania Di Cio, Thea M L Bøggild, John Connelly, Duncan S Sutherland, Julien E Gautrot
: The nanoscale geometry and topography of the extra-cellular matrix (ECM) is an important parameter controlling cell adhesion and phenotype. Similarly, integrin expression and the geometrical maturation of adhesions they regulate have been correlated with important changes in cell spreading and phenotype. However, how integrin expression controls the nanoscale sensing of the ECM geometry is not clearly understood. Here we develop a new nanopatterning technique, electrospun nanofiber lithography (ENL), which allows the production of a quasi-2D fibrous nanopattern with controlled dimensions (250-1000nm) and densities...
December 8, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27939890/lumican-null-mice-are-susceptible-to-aging-and-isoproterenol-induced-myocardial-fibrosis
#12
Shao-Wei Chen, Ying-Chang Tung, Shih-Ming Jung, Yen Chu, Pyng-Jing Lin, Winston W-Y Kao, Pao-Hsien Chu
With aging and stress, the myocardium undergoes structural remodeling, often leading to fibrosis. The purpose of this study is to examine whether lumican, one of the class II small leucine-rich proteoglycans, has a protective role in cardiac remodeling and fibrosis. In attempts to elucidate the hypothesis that lumican may have a protective role in cardiac remodeling and fibrosis, we compared the cardiac phenotypes of young (3-month-old) and elder (6-month- and 12-month-old) lumican-null (Lum(-/-)) mice. Extra-cellular matrix remodeling and apoptosis are examined to determine the roles of lumican on age-dependent cardiac fibrosis induced by isoproterenol...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27928505/collagen-a-network-for-regenerative-medicine
#13
REVIEW
K M Pawelec, S M Best, R E Cameron
The basic building block of the extra-cellular matrix in native tissue is collagen. As a structural protein, collagen has an inherent biocompatibility making it an ideal material for regenerative medicine. Cellular response, mediated by integrins, is dictated by the structure and chemistry of the collagen fibers. Fiber formation, via fibrillogenesis, can be controlled in vitro by several factors: pH, ionic strength, and collagen structure. After formation, fibers are stabilized via cross-linking. The final bioactivity of collagen scaffolds is a result of both processes...
October 28, 2016: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/27916739/bioengineered-tumoral-microtissues-recapitulate-desmoplastic-reaction-of-pancreatic-cancer
#14
Virginia Brancato, Valentina Comunanza, Giorgia Imparato, Davide Corà, Francesco Urciuolo, Alessio Noghero, Federico Bussolino, Paolo A Netti
: Many of the existing three-dimensional (3D) cancer models in vitro fail to represent the entire complex tumor microenvironment composed of cells and extra cellular matrix (ECM) and do not allow a reliable study of the tumoral features and progression. In this paper we reported a strategy to produce 3D in vitro microtissues of pancreatic ductal adenocarcinoma (PDAC) for studying the desmoplastic reaction activated by the stroma-cancer crosstalk. Human PDAC microtissues were obtained by co-culturing pancreatic cancer cells (PT45) and normal or cancer-associated fibroblasts within biodegradable microcarriers in a spinner flask bioreactor...
December 1, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#15
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
November 30, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27889145/potential-probiotic-pichia-kudriavzevii-strains-and-their-ability-to-enhance-folate-content-of-traditional-cereal-based-african-fermented-food
#16
Anna Greppi, Fabien Saubade, Cristian Botta, Christèle Humblot, Jean-Pierre Guyot, Luca Cocolin
With the aim of selecting starter cultures with interesting probiotic potential and with the ability to produce folate in a food matrix, yeast strains isolated from fermented cereal-based African foods were investigated. A total of 93 yeast strains were screened for their tolerance to pH 2 and 0.3% of bile salts. Pichia kudriavzevii isolates gave the best results. Selected P. kudriavzevii strains were tested for survival to the simulated human digestion and for adhesion to Caco-2 cells. Moreover, presence of folate biosynthesis genes was verified and production of extra and intra-cellular folate determined during growth in culture medium...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27876673/extra-cellular-matrix-a-modular-soil-for-stem-cells
#17
EDITORIAL
Irmgard Irminger-Finger, Joanna Kargul, Geoffrey J Laurent
No abstract text is available yet for this article.
December 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27871028/microstructure-analysis-method-for-evaluating-degenerated-intervertebral-disc-tissue
#18
Andrea Friedmann, Felix Goehre, Christopher Ludtka, Thomas Mendel, Hans-Joerg Meisel, Andreas Heilmann, Stefan Schwan
Degeneration of intervertebral disc (IVD) tissue is characterized by several structural changes that result in variations in disc physiology and loss of biomechanical function. The complex process of degeneration exhibits highly intercorrelated biomechanical, biochemical, and cellular interactions. There is currently some understanding of the cellular changes in degenerated intervertebral disc tissue, but microstructural changes and deterioration of the tissue matrix has previously been rarely explored. In this work, sequestered IVD tissue was successfully characterized using histology, light microscopy, and scanning electron microscopy (SEM) to quantitatively evaluate parameters of interest for intervertebral disc degeneration (IDD) such as delamination of the collagenous matrix, cell density, cell size, and extra cellular matrix (ECM) thickness...
October 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27861153/epithelial-derived-matrix-metalloproteinase-mmp9-exhibits-a-novel-defensive-role-of-tumor-suppressor-in-colitis-associated-cancer-by-activating-mmp9-notch1-arf-p53-axis
#19
Lewins Walter, Adani Pujada, Noopur Bhatnagar, Agnieszka B Bialkowska, Vincent W Yang, Hamed Laroui, Pallavi Garg
Colitis associated cancer (CAC) is chronic inflammation driven colon cancer, prevalent among individuals with Inflammatory Bowel Disease. Matrix-metalloproteinase (MMP9) is one of the essential regulators of extra cellular matrix components. We have shown that MMP9 is protective in CAC contrary to its inflammatory role in acute-colitis. Aim of our study is to identify the mechanism of the protective role of epithelial derived-MMP9 in CAC. We used homozygous transgenic mice constitutively-expressing MMP9 in colonic-epithelium (TgM9) and wild-type (WT) littermates for in vivo experiments...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27856309/hedgehog-signalling-acts-upstream-of-laminin-alpha1-transcription-in-the-zebrafish-paraxial-mesoderm
#20
Joseph Pickering, Vincent T Cunliffe, Freek Van Eeden, Anne-Gaëlle Borycki
Laminin-111 (α1β1γ1) is a member of the Laminin family of extra-cellular matrix proteins that comprises 16 members, components of basement membranes. Laminin-111, one of the first Laminin proteins synthesised during embryogenesis, is required for basement membrane deposition and has essential roles in tissue morphogenesis and patterning. Yet, the mechanisms controlling Laminin-111 expression are poorly understood. We generated a zebrafish transgenic reporter line that reproduces faithfully the expression pattern of lama1, the gene encoding Laminin α1, and we used this reporter line to investigate lama1 transcriptional regulation...
November 14, 2016: Matrix Biology: Journal of the International Society for Matrix Biology
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