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https://www.readbyqxmd.com/read/29679778/recapitulating-spatiotemporal-tumor-heterogeneity-in-vitro-through-engineered-breast-cancer-microtissues
#1
Claudia Mazio, Costantino Casale, Giorgia Imparato, Francesco Urciuolo, Paolo Antonio Netti
Tumor and microenvironmental heterogeneity hinders the study of breast cancer biology and the assessment of therapeutic strategies, being associated with high variability and drug resistance. In this context, it is mandatory to develop three-dimensional breast tumor models able to reproduce this heterogeneity and the dynamic interaction occurring between tumor cells and microenvironment. Here we show a new breast cancer microtissue model (T-µTP) uniquely able to present intra-tumor morphological heterogeneity in a dynamic and responsive endogenous matrix...
April 18, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29649568/honokiol-alleviates-the-degeneration-of-intervertebral-disc-via-suppressing-the-activation-of-txnip-nlrp3-inflammasome-signal-pathway
#2
Pan Tang, Jia-Ming Gu, Zi-Ang Xie, Yu Gu, Zhi-Wei Jie, Kang-Mao Huang, Ji-Ying Wang, Shun-Wu Fan, Xue-Sheng Jiang, Zhi-Jun Hu
Intervertebral disc degeneration (IVDD) is a multifactorial disease and responsible for many spine related disorders, causes disability in the workforce and heavy social costs all over the world. Honokiol, a low molecular weight natural product, could penetrate into and distribute in IVDs to achieve therapeutic effect in a rat tail model. Therefore, the present study was undertaken to examine the antiinflammatory, antioxidation and chondroprotective effect of honokiol using nucleus pulposus cells and investigate its mechanisms to provide a new basis for future clinical treatment of IVDD...
April 9, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29643414/switching-between-individual-and-collective-motility-in-b-lymphocytes-is-controlled-by-cell-matrix-adhesion-and-inter-cellular-interactions
#3
Javier Rey-Barroso, Daniel S Calovi, Maud Combe, Yolla German, Mathieu Moreau, Astrid Canivet, Xiaobo Wang, Clément Sire, Guy Theraulaz, Loïc Dupré
Lymphocytes alternate between phases of individual migration across tissues and phases of clustering during activation and function. The range of lymphocyte motility behaviors and the identity of the factors that govern them remain elusive. To explore this point, we here collected unprecedented statistics pertaining to cell displacements, cell:matrix and cell:cell interactions using a model B cell line as well as primary human B lymphocytes. At low cell density, individual B lymphocytes displayed a high heterogeneity in their speed and diffusivity...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29629612/the-9p21-locus-as-a-potential-therapeutic-target-and-prognostic-marker-in-colorectal-cancer
#4
Afsane Bahrami, Seyed Mahdi Hassanian, Majid Khazaei, Masoumeh Gharib, Mahsa Rahmani, Hamid Fiuji, Mir Hadi Jazayeri, Mehrdad Moetamani-Ahmadi, Gordon A Ferns, Amir Avan
Colorectal cancer (CRC) is a major cause of cancer-related-death worldwide. Despite extensive efforts to identify valid biomarkers for the risk stratification of CRC patients, there are few of proven clinical utility. It is recognized that genetic factors play a major role in determining susceptibility to CRC. Recent genome-wide association studies have demonstrated common genetic variants in a region on chromosome 9p21 associated with an increased risk of CRC. Several genetic polymorphisms have been identified in this region that are associated with CRC...
April 9, 2018: Pharmacogenomics
https://www.readbyqxmd.com/read/29615492/surface-exposed-loops-and-an-acidic-patch-in-the-scl1-protein-of-group-a-streptococcus-enable-scl1-binding-to-wound-associated-fibronectin
#5
Dudley H McNitt, Soo Jeon Choi, Douglas R Keene, Livingston Van De Water, Flavia Squeglia, Rita Berisio, Slawomir Lukomski
Keratinized epidermis constitutes a powerful barrier of the mucosa and skin, effectively preventing bacterial invasion, unless it is wounded and no longer protective. Wound healing involves deposition of distinct extracellular matrix (ECM) proteins enriched in cellular fibronectin (cFn) isoforms containing extra domain A (EDA). The streptococcal collagen-like protein 1 (Scl1) is a surface adhesin of group A Streptococcus (GAS), which contains an N-terminal variable (V) domain and a C-terminally located collagen-like domain...
April 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29597130/microrna-co-expression-patterns-unravel-the-relevance-of-extra-cellular-matrix-and-immunity-in-breast-cancer
#6
Matteo Dugo, Xiaoyan Huang, Marilena V Iorio, Alessandra Cataldo, Elda Tagliabue, Maria Grazia Daidone, Jiong Wu, Rosaria Orlandi
BACKGROUND: Aberrant microRNA (miRNA) expression is associated with human tumors, including breast cancer (BC), and has diagnostic and therapeutic potential. BC tissue is characterized by distinctive miRNA signatures associated with cancer development or progression. METHODS: We explored miRNA profiles from BC tissues by unsupervised hierarchical clustering. Functional analysis was based on miRNA-mRNA data integration and Gene Set Enrichment Analysis. RESULTS: Partition of miRNA expression data from BC samples of Chinese Han and Caucasian subjects identified clusters of correlated miRNAs that were subjected to a global and integrative analysis aimed at identifying novel associations with biological and clinical value...
March 26, 2018: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/29582951/-from-the-lab-bench-to-the-patient-can-injured-skeletal-tissues-regenerate
#7
REVIEW
Amir Haze, Josh Schroeder, Anat Blumenfeld, Meir Liebergall, Shaul Beyth
Regenerative medicine research has evolved significantly in recent years. There is a great un-met clinical need for developing new treatments that will induce regeneration of injured skeletal tissues in cases such as large bony defects caused by trauma or tumor resection, articular cartilage defects and torn or degenerate tendons and ligaments. Except for bone that can regenerate small defects, all other skeletal tissues do not hold the natural capability for regeneration after injury and rather form a less functional scar tissue...
March 2018: Harefuah
https://www.readbyqxmd.com/read/29569168/development-of-decellularized-amniotic-membrane-as-a-bioscaffold-for-bone-marrow-derived-mesenchymal-stem-cells-ultrastructural-study
#8
Radwa Ayman Salah, Ihab K Mohamed, Nagwa El-Badri
Developing effective stem cell-based therapies requires the design of complex in vitro culture systems for accurate representation of the physiological stem cell niche. Human amniotic membrane (hAM) has been successfully used in clinical grafting applications due to its unique biological and regenerative properties. Decellularized hAM (d-hAM) has been previously applied to the culture of human bone marrow mesenchymal stem cells (hMSCs), promoting their expansion and differentiation into adipogenic and osteogenic lineages...
March 22, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29559838/decellularized-hydrogels-in-bone-tissue-engineering-a-topical-review
#9
REVIEW
Andrea Pacifici, Luigi Laino, Marco Gargari, Federico Guzzo, Andrea Velandia Luz, Antonella Polimeni, Luciano Pacifici
Nowadays, autograft and allograft techniques represent the main solution to improve bone repair. Unfortunately, autograft technique is expensive, invasive and subject to infections and hematoma, frequently affecting both donor sites and surgical sites. A recent advance in tissue engineering is the fabrication of cell-laden hydrogels with custom-made geometry, depending on the clinical case. The use of ECM (Extra-Cellular Matrix)-derived Hydrogels from bone tissue is the new opportunity to obtain good results in bone regeneration...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29540680/basal-lamina-remodeling-at-the-skeletal-muscle-stem-cell-niche-mediates-stem-cell-self-renewal
#10
Shantisree Sandeepani Rayagiri, Daniele Ranaldi, Alexander Raven, Nur Izzah Farhana Mohamad Azhar, Olivier Lefebvre, Peter S Zammit, Anne-Gaëlle Borycki
A central question in stem cell biology is the relationship between stem cells and their niche. Although previous reports have uncovered how signaling molecules released by niche cells support stem cell function, the role of the extra-cellular matrix (ECM) within the niche is unclear. Here, we show that upon activation, skeletal muscle stem cells (satellite cells) induce local remodeling of the ECM and the deposition of laminin-α1 and laminin-α5 into the basal lamina of the satellite cell niche. Genetic ablation of laminin-α1, disruption of integrin-α6 signaling or blocking matrix metalloproteinase activity impairs satellite cell expansion and self-renewal...
March 14, 2018: Nature Communications
https://www.readbyqxmd.com/read/29536321/assessment-of-remote-myocardium-heterogeneity-in-patients-with-ventricular-tachycardia-using-texture-analysis-of-late-iodine-enhancement-lie-cardiac-computed-tomography-cct-images
#11
Antonio Esposito, Anna Palmisano, Sofia Antunes, Caterina Colantoni, Paola Maria Vittoria Rancoita, Davide Vignale, Francesca Baratto, Paolo Della Bella, Alessandro Del Maschio, Francesco De Cobelli
PURPOSE: Diffuse remodeling of myocardial extra-cellular matrix is largely responsible for left ventricle (LV) dysfunction and arrhythmias. Our hypothesis is that the texture analysis of late iodine enhancement (LIE) cardiac computed tomography (cCT) images may improve characterization of the diffuse extra-cellular matrix changes. Our aim was to extract volumetric extracellular volume (ECV) and LIE texture features of non-scarred (remote) myocardium from cCT of patients with recurrent ventricular tachycardia (rVT), and to compare these radiomic features with LV-function, LV-remodeling, and underlying cardiac disease...
March 13, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29523136/characterisation-of-feline-renal-cortical-fibroblast-cultures-and-their-transcriptional-response-to-transforming-growth-factor-%C3%AE-1
#12
J S Lawson, H M Syme, C P D Wheeler-Jones, J Elliott
BACKGROUND: Chronic kidney disease (CKD) is common in geriatric cats, and the most prevalent pathology is chronic tubulointerstitial inflammation and fibrosis. The cell type predominantly responsible for the production of extra-cellular matrix in renal fibrosis is the myofibroblast, and fibroblast to myofibroblast differentiation is probably a crucial event. The cytokine TGF-β1 is reportedly the most important regulator of myofibroblastic differentiation in other species. The aim of this study was to isolate and characterise renal fibroblasts from cadaverous kidney tissue of cats with and without CKD, and to investigate the transcriptional response to TGF-β1...
March 9, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29506630/human-podocytes-responses-to-alternatively-spliced-extra-domain-a-fibronectin-in-culture
#13
Tarunkumar Hemraj Madne, Mark Edward Carl Dockrell
The interactions of the extracellular matrix (ECM) proteins with cells strongly regulate cell behaviour. The glomerular basement membrane (GBM) is a dynamic structure made up of protein secreted by endothelial cells and podocyte. These proteins could regulate the behaviour of these cells in health and diseases. Extra Domain A + Fn (EDA+Fn) is an alternatively spliced form of Fibronectin (Fn) recently identified in GBM and a recognised marker of various pathologies. In this study for the first time, we have investigated the responses of human podocytes cultured on different composition of GBM proteins which are cellular Fn (EDA+), plasma Fn (EDA-) and collagen IV...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29506626/tgf%C3%AE-1-mediated-expression-and-alternative-splicing-of-fibronectin-extra-domain-a-in-human-podocyte-culture
#14
Tarunkumar Hemraj Madne, Mark Edward Carl Dockrell
Alternative splicing is a fundamental phenomenon to build protein diversity in health and diseases. Extra Domain A+ Fibronectin (EDA+Fn) is an alternatively spliced form of fibronectin protein present in the extra cellular matrix (ECM) in renal fibrosis. Podocytes are spectacular cell type and play a key role in filtration and synthesise ECM proteins in renal physiology and pathology. TGFβ1 is a strong stimulator of ECM proteins in renal injury. In this study, we have investigated alternative splicing of EDA+ Fn in human podocytes in response to TGFβ1...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29468965/versatility-of-cancer-associated-fibroblasts-commendable-targets-for-anti-tumor-therapy
#15
Ghulam Jilany Khan, Li Sun, Sara Khan, Shengtao Yuan, He Nongyue
BACKGROUND AND OBJECTIVE: Tumor growth, development, progression, metastasis, invasion and resistivity against drugs, solely depends on the recruitment of surrounding cells, extra cellular matrix and tumor microenvironment (TME). TME is directly as well as indirectly associated with cancer associated fibroblasts (CAF). The interactions of CAF with tumor surrounding cells, their origination, activation, transformation and ability to recruit other non-tumor cells for the clemency of tumor, are very complex and under active research since long...
February 19, 2018: Current Drug Targets
https://www.readbyqxmd.com/read/29458111/ppar%C3%AE-modulation-rescues-mitochondrial-fatty-acid-oxidation-defects-in-the-mdx-model-of-muscular-dystrophy
#16
Eric L Bell, Robert W Shine, Peter Dwyer, Lyndsay Olson, Jennifer Truong, Ross Fredenburg, Matthew Goddeeris, Dominique Stickens, Effie Tozzo
Duchenne muscular dystrophy (DMD) is a recessive, fatal X-linked disease that is characterized by progressive skeletal muscle wasting due to the absence of dystrophin, which is an a essential protein that bridges the inner cytoskeleton and extra-cellular matrix. This study set out to characterize the mitochondria in primary muscle satellite cell derived myoblasts from mdx mice and wild type control mice. Compared to wild type derived cells the mdx derived cells have reduced mitochondrial bioenergetics and have fewer mitochondria...
February 16, 2018: Mitochondrion
https://www.readbyqxmd.com/read/29454947/guide-to-electrospinning-denatured-whole-chain-collagen-from-hoki-fish-using-benign-solvents
#17
Deborah Le Corre-Bordes, Kathleen Hofman, Bronwyn Hall
Tissue engineering requires the design and manufacture of biomimetic scaffolds. Collagen-derived nanofibrous scaffolds have been intensively studied because collagen, in the form of fibrils, is one of the main components of the extra cellular matrix (ECM). Several collagen materials have been used in electrospinning studies including mammalian extracted Type I collagen and gelatin formulations. Denatured whole chain collagen (DWCC) can be prepared by heat denaturing acid-soluble collagen extracted from cold-water fish skin...
February 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29421060/alginate-polymethacrylate-hybrid-hydrogels-for-potential-osteochondral-tissue-regeneration
#18
P Stagnaro, I Schizzi, R Utzeri, E Marsano, M Castellano
Porous scaffolds based on alginate-polymethacrylate hybrid hydrogels intended for bone and cartilage regeneration were prepared through controlled calcium ions diffusion from an agar mould. The double interconnected network of such materials combines into a single porous structure maintained by both noncovalent crosslinks (calcium ions for alginate) and covalent crosslinks (polymethacrylate crosslinked by the addition of mixtures of mono and bifunctional monomers). The alginate component ensures the appropriate micro-environment to mimic the extra-cellular matrix, whereas the polymethacrylate improves the mechanical performances of the hybrid hydrogels, helping to overcome the mechanical limitations of the alginate component...
April 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29420170/jak-stat-pathway-induces-drosophila-follicle-elongation-by-a-gradient-of-apical-contractility
#19
Hervé Alégot, Pierre Pouchin, Olivier Bardot, Vincent Mirouse
Tissue elongation and its control by spatiotemporal signals is a major developmental question. Currently, it is thought that Drosophila ovarian follicular epithelium elongation requires the planar polarization of the basal domain cytoskeleton and of the extra-cellular matrix, associated with a dynamic process of rotation around the anteroposterior axis. Here we show, by careful kinetic analysis of fat2 mutants, that neither basal planar polarization nor rotation is required during a first phase of follicle elongation...
February 8, 2018: ELife
https://www.readbyqxmd.com/read/29402544/-facial-injections-of-hyaluronic-acid-based-fillers-for-malformations-preliminary-study-regarding-scar-tissue-improvement-and-cosmetic-betterment
#20
G Franchi, C Neiva-Vaz, A Picard, M-P Vazquez
BACKGROUND: Cross-linked hyaluronic acid-based fillers have gained rapid acceptance for treating facial wrinkles, deep tissue folds and sunken areas due to aging. This study evaluates, in addition to space-filling properties, their effects on softness and elasticity as a secondary effect, following injection of 3 commercially available cross-linked hyaluronic acid-based fillers (15mg/mL, 17,5mg/mL and 20mg/mL) in patients presenting with congenital or acquired facial malformations. PATIENTS ET METHODS: We started injecting gels of cross-linked hyaluronic acid-based fillers in those cases in 2013; we performed 46 sessions of injections in 32 patients, aged from 13-32...
February 2, 2018: Annales de Chirurgie Plastique et Esthétique
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