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Adipose derived mesenchymal stem cells

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https://www.readbyqxmd.com/read/29925829/in-vitro-expression-of-cytokeratin-19-in-adipose-derived-stem-cells-is-induced-by-epidermal-growth-factor
#1
Shangliang Chen, Mingzhu Wang, Xinglu Chen, Shaolian Chen, Li Liu, Jianbin Zhu, Jinhui Wang, Xiaorong Yang, Xiangsheng Cai
BACKGROUND Cytokeratin 19 (CK19) is a typical epithelial marker. In this study, we determined whether epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) could enhance CK19 expression in adipose-derived stem cells (ADSCs), thereby inducing the differentiation of ADSCs into epithelial-like cells. MATERIAL AND METHODS ADSCs were isolated from perinephric fat, and the expression of CD29, CD90, and CD105 was confirmed. Following isolation, ADSCs were cultured in static medium or medium containing EGF or bFGF...
June 21, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29923191/effects-of-endurance-exercise-training-on-inflammatory-circulating-progenitor-cell-content-in-lean-and-obese-adults
#2
Grace M Niemiro, Jacob M Allen, Lucy J Mailing, Naiman A Khan, Hannah D Holscher, Jeffrey A Woods, Michael De Lisio
KEY POINTS: Chronic inflammation underlies many of the health decrements associated with obesity. Circulating progenitor cells can sense and respond to inflammatory stimuli, increasing the local inflammatory response within tissues. Here we show that 6 weeks of endurance exercise training significantly decreases inflammatory circulating progenitor cells in obese adults. These findings provide novel cellular mechanisms for the beneficial effects of exercise in obese adults. ABSTRACT: Circulating progenitor cells (CPCs) and subpopulations are normally found in the bone marrow, but can migrate to peripheral tissues to participate in local inflammation and/or remodelling...
June 19, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29922425/an-answer-to-colon-cancer-treatment-by-mesenchymal-stem-cell-originated-from-adipose-tissue
#3
Elif Sinem Iplik, Baris Ertugrul, Ilknur Kozanoglu, Yusuf Baran, Bedia Cakmakoglu
Objectives: Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies...
May 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29921325/inhibition-of-the-rhogtpase-cdc42-by-ml141-enhances-hepatocyte-differentiation-from-human-adipose-derived-mesenchymal-stem-cells-via-the-wnt5a-pi3k-mir-122-pathway-impact-of-the-age-of-the-donor
#4
Diana Chaker, Charbel Mouawad, Albert Azar, Didier Quilliot, Ibrahim Achkar, Ziad Fajloun, Nehman Makdissy
BACKGROUND: Human adipose-derived mesenchymal stem cells (hADSCs) are promising cells that may promote hepatocyte differentiation (Hep-Dif) and improve liver function, but the involvement of Cdc42, a key small RhoGTPase which plays a crucial role in aging, is still not well established. We hypothesized that the inhibition of Cdc42 may rescue the hepatogenic potential of hADSCs derived from aged donors. METHODS: hADSCs isolated from 61 women of different ages were cultured for evaluation of the proliferation of cells, adherence, apoptosis, immunomodulation, immunophenotyping, multipotency, gene expression, and cell function during Hep-Dif...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29921311/adipose-derived-and-bone-marrow-mesenchymal-stem-cells-a-donor-matched-comparison
#5
Samih Mohamed-Ahmed, Inge Fristad, Stein Atle Lie, Salwa Suliman, Kamal Mustafa, Hallvard Vindenes, Shaza B Idris
BACKGROUND: Adipose-derived stem cells (ASCs) have been introduced as an alternative to bone marrow mesenchymal stem cells (BMSCs) for cell-based therapy. However, different studies comparing ASCs and BMSCs have shown conflicting results. In fact, harvesting ASCs and BMSCs from different individuals might influence the results, making comparison difficult. Therefore, this study aimed to characterize donor-matched ASCs and BMSCs in order to investigate proliferation, differentiation potential and possible effects of donor variation on these mesenchymal stem cells (MSCs)...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29920999/anti-apoptotic-effects-of-adipose-derived-adherent-stromal-cells-in-mesenchymal-stem-cells-exposed-to-oxidative-stress
#6
Sunhye Shin, Jung-Won Choi, Soyeon Lim, Seahyoung Lee, Eun-Young Jun, Hyun-Min Sun, Il-Kwon Kim, Hoon-Bum Lee, Sang Woo Kim, Ki-Chul Hwang
Adipose-derived stromal vascular fractions (SVFs) are a heterogeneous collection of cells, and their regenerative modality has been applied in various animal experiments and clinical trials. Despite the attractive advantages of SVFs in clinical interventions, the recent status of clinical studies involving the application of SVFs in many diseases has not been fully evaluated. Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types despite their low numbers in heart tissue...
June 19, 2018: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29909687/comparative-efficacy-of-autologous-stromal-vascular-fraction-and-autologous-adipose-derived-mesenchymal-stem-cells-combined-with-hyaluronic-acid-for-the-treatment-of-sheep-osteoarthritis
#7
Xiaoteng Lv, Jiyin He, Xue Zhang, Xuan Luo, Na He, Zhongwei Sun, Huitang Xia, Victor Liu, Li Zhang, Xiangming Lin, Liping Lin, Huabin Yin, Dong Jiang, Wei Cao, Richard Wang, Guangdong Zhou, Wen Wang
The current study explored whether intra-articular (IA) injection of autologous adipose mesenchymal stem cells (ASCs) combined with hyaluronic acid (HA) achieved better therapeutic efficacy than autologous stromal vascular fraction (SVF) combined with HA to prevent osteoarthritis (OA) progression and determined how long autologous ASCs combined with HA must remain in the joint to observe efficacy. OA models were established by performing anterior cruciate ligament transection (ACLT) and medial meniscectomy (MM)...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29909686/improved-healing-after-the-co-transplantation-of-ho-1-and-bdnf-overexpressed-mesenchymal-stem-cells-in-the-subacute-spinal-cord-injury-of-dogs
#8
Imdad Ullah Khan, Yongseok Yoon, Ahyoung Kim, Kwang Rae Jo, Kyeung Uk Choi, Taeseong Jung, Namyul Kim, YeonSung Son, Wan Hee Kim, Oh-Kyeong Kweon
Abundant expression of proinflammatory cytokines after a spinal cord injury (SCI) creates an inhibitory microenvironment for neuroregeneration. The mesenchymal stem cells help to mitigate the inflammation and improve neural growth and survival. For this purpose, we potentiated the function of adipose-derived mesenchymal stem cells (Ad-MSCs) by transfecting them with brain-derived neurotrophic factor (BDNF) and heme oxygenase-1 (HO-1), through a lentivirus, to produce BDNF overexpressed Ad-MSCs (BDNF-MSCs), and HO-1 overexpressed Ad-MSCs (HO-1-MSCs)...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29908226/visfatin-alters-the-cytokine-and-matrix-degrading-enzyme-profile-during-osteogenic-and-adipogenic-msc-differentiation
#9
Lali Tsiklauri, Janina Werner, Marian Kampschulte, Klaus W Frommer, Lucija Berninger, Martina Irrgang, Kristina Glenske, Dirk Hose, Thaqif El Khassawna, Jörn Pons-Kühnemann, Stefan Rehart, Sabine Wenisch, Ulf Müller-Ladner, Elena Neumann
OBJECTIVES: Age-related bone loss is associated with bone marrow adiposity. Adipokines (e.g. visfatin, resistin, leptin) are adipocyte-derived factors with immunomodulatory properties and might influence differentiation of bone marrow-derived mesenchymal stem cells (MSC) in osteoarthritis (OA) and osteoporosis. Thus, the presence of adipokines and MMPs in bone marrow and their effects on MSC differentiation were analyzed. METHODS: MSC and RNA were isolated from femoral heads after hip replacement surgery of OA or osteoporotic femoral neck fracture (FF) patients...
June 13, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29905790/characterization-of-the-tissue-and-stromal-cell-components-of-micro-superficial-enhanced-fluid-fat-injection-micro-seffi-for-facial-aging-treatment
#10
Martina Rossi, Barbara Roda, Silvia Zia, Ilaria Vigliotta, Chiara Zannini, Francesco Alviano, Laura Bonsi, Andrea Zattoni, Pierluigi Reschiglian, Alessandro Gennai
Background: New microfat preparations provide material suitable for use as a regenerative filler for different facial areas. To support the development of new robust techniques for regenerative purposes, the cellular content of the sample should be considered. Objectives: To evaluate the stromal vascular fraction (SVF) cell components of micro-superficial enhanced fluid fat injection (SEFFI) samples via a technique to harvest re-injectable tissue with minimum manipulation...
June 14, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29904354/paracrine-anti-inflammatory-effects-of-adipose-tissue-derived-mesenchymal-stem-cells-in-human-monocytes
#11
Maria I Guillén, Julia Platas, María D Pérez Del Caz, Vicente Mirabet, Maria J Alcaraz
The inflammatory process is an essential phenomenon in the induction of immune responses. Monocytes are key effector cells during the inflammatory process. A wide range of evidence indicates that mesenchymal stem cells from adipose tissue (ASC) are endowed with immunomodulatory capacity. However, the interaction between ASC and monocytes in the innate immune response is not well understood. The aim of this work was to investigate the possible paracrine anti-inflammatory effects of ASC in human monocytes. Monocytes were isolated from buffy coats and ASC from fat of non-obese patients...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29901284/application-of-tissue-engineered-pericardial-patch-in-rat-models-of-myocardial-infarction
#12
Maryam Kameli, Reza Khorramirouz, Sahar Eftekharzadeh, Kiarad Fendereski, Seyedeh Sima Daryabari, Seyed Mohammad Tavangar, Abdol-Mohammad Kajbafzadeh
INTRODUCTION: Myocardial infarction (MI) is a major cause of mortality and morbidity in industrialized societies. Myocardial tissue engineering is an alternative and promising approach for substituting injured myocardium through development and seeding of appropriate scaffolds. In this study, we investigated the efficacy of using an acellular pericardium to deliver autologous mesenchymal stem cells (MSCs) to the infarcted site for regeneration of the myocardium. MATERIALS AND METHODS: MI was induced in two groups of rats; G1 or MI group, and G2 or patch-implanted group...
June 14, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29898789/co-stimulation-of-lpar-1-and-s1pr-1-3-increases-the-transplantation-efficacy-of-human-mesenchymal-stem-cells-in-drug-induced-and-alcoholic-liver-diseases
#13
Mianhuan Li, Yi Lv, Feng Chen, Xiaoyan Wang, Jiang Zhu, Hao Li, Jia Xiao
BACKGROUND: One of the major obstacles facing stem cell therapy is the limited number of functional stem cells available after transplantation due to the harsh microenvironment surrounding the damaged tissue. The aim of this study was to delineate the mechanistic involvement of lysophosphatidic acid receptors (LPARs) and sphingosine-1-phosphate receptors (S1PRs) in the regulation of anti-stress and transplantation efficacy of stem cells. METHODS: Human adipose-derived mesenchymal stem cells (hADMSCs) were treated with chemical toxin or ethanol to induce cell stress...
June 14, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29898623/depletion-of-sirt3-leads-to-the-impairment-of-adipogenic-differentiation-and-insulin-resistance-via-interfering-mitochondrial-function-of-adipose-derived-human-mesenchymal-stem-cells
#14
Yu-Ting Wu, Kun-Ting Chi, Yueh-Wen Lan, Jui-Chi Chan, Yi-Shing Ma, Yau-Huei Wei
Upregulation of mitochondrial function and oxidative metabolism is a hallmark in the differentiation of stem cells. However, the mechanism underlying the metabolic reprogramming and upregulation of mitochondrial function during the differentiation of human mesenchymal stem cells (hMSCs) is largely unclear. Sirt3 has emerged as a sensor in regulating mitochondrial function and antioxidant defense system in cellular response to energy demand or environmental stimuli, but its roles in stem cell differentiation has not been fully understood...
June 14, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29895333/exosomes-secreted-by-adipose-derived-mesenchymal-stem-cells-regulate-type-i-collagen-metabolism-in-fibroblasts-from-women-with-stress-urinary-incontinence
#15
Xiaochun Liu, Shiwei Wang, Suhui Wu, Qian Hao, Ying Li, Zhuodan Guo, Wenzhen Wang
BACKGROUND: Mesenchymal stem cells (MSC) have gained credibility as a therapeutic tool partly due to their potential to secrete factors such as cytokines and chemokines. Recently, exosomes, which mediate intercellular communication by delivering biomolecules such as mRNA and miRNA into recipient cells, have gained attention as a new and valuable therapeutic strategy in regenerative medicine. However, the potential role of exosomes secreted by adipose-derived mesenchymal stem cells (adMSC-Exos) in collagen metabolism is not well understood...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29892899/microarray-analysis-after-adipose-derived-mesenchymal-stem-cells-injection-in-monosodium-iodoacetate-induced-osteoarthritis-rats
#16
Jae Chul Lee
Osteoarthritis (OA) is a degenerative joint disease characterized by abrasion, and ultimately, destruction of the articular cartilage and trabecular bone loss. OA is still considered a devastating disease, which requires an aggressive therapeutic approach. Despite the therapeutic potential of human adipose-derived mesenchymal stem cells (AD-MSCs), the molecular parameters needed to define the "stemness" remain largely unknown. Using high-density oligonucleotide microarrays, the differential gene expression profiles between a fraction of human adipose-derived (AD) mononuclear cells and its MSC subpopulation were obtained...
January 2018: Genes & Genomics
https://www.readbyqxmd.com/read/29891848/fas-l-promotes-the-stem-cell-potency-of-adipose-derived-mesenchymal-cells
#17
Inna Solodeev, Benjamin Meilik, Ilan Volovitz, Meirav Sela, Sharon Manheim, Shai Yarkoni, Dov Zipori, Eyal Gur, Nir Shani
Fas-L is a TNF family member known to trigger cell death. It has recently become evident that Fas-L can transduce also non-apoptotic signals. Mesenchymal stem cells (MSCs) are multipotent cells that are derived from various adult tissues. Although MSCs from different tissues display common properties they also display tissue-specific characteristics. Previous works have demonstrated massive apoptosis following Fas-L treatment of bone marrow-derived MSCs both in vitro and following their administration in vivo...
June 11, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29890767/effect-of-different-preconditioning-regimens-on-the-expression-profile-of-murine-adipose-derived-stromal-stem-cells
#18
Patrick C Baer, Jürgen M Overath, Anja Urbschat, Ralf Schubert, Benjamin Koch, Asanke A Bohn, Helmut Geiger
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens are considered to be a promising strategy to improve the release of regenerative factors. In the present study, MSC were isolated from inguinal adipose tissue (mASC) from C57BL/6 mice, cultured, and characterized. Then, mASC were either preconditioned by incubation in a hypoxic environment (0...
June 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29890754/nanoscale-electrical-potential-and-roughness-of-a-calcium-phosphate-surface-promotes-the-osteogenic-phenotype-of-stromal-cells
#19
Igor A Khlusov, Yuri Dekhtyar, Yurii P Sharkeev, Vladimir F Pichugin, Marina Y Khlusova, Nataliya Polyaka, Fedor Tyulkin, Viktorija Vendinya, Elena V Legostaeva, Larisa S Litvinova, Valeria V Shupletsova, Olga G Khaziakhmatova, Kristina A Yurova, Konstantin A Prosolov
Mesenchymal stem cells (MSCs) and osteoblasts respond to the surface electrical charge and topography of biomaterials. This work focuses on the connection between the roughness of calcium phosphate (CP) surfaces and their electrical potential (EP) at the micro- and nanoscales and the possible role of these parameters in jointly affecting human MSC osteogenic differentiation and maturation in vitro. A microarc CP coating was deposited on titanium substrates and characterized at the micro- and nanoscale. Human adult adipose-derived MSCs (hAMSCs) or prenatal stromal cells from the human lung (HLPSCs) were cultured on the CP surface to estimate MSC behavior...
June 9, 2018: Materials
https://www.readbyqxmd.com/read/29889737/a-simple-dynamic-strategy-to-deliver-stem-cells-to-decellularized-nerve-allografts
#20
Nadia Rbia, Liselotte F Bulstra, Allen T Bishop, Andre J van Wijnen, Alexander Y Shin
BACKGROUND: The addition of adipose-derived Mesenchymal Stromal Cells (MSCs) to decellularized nerve allografts may improve outcomes of nerve reconstruction. Prior techniques used for cell seeding are traumatic to both the MSCs and nerve graft. An adequate, reliable and validated cell seeding technique is an essential step for evaluating the translational utility of MSC-enhanced decellularized nerve grafts. The purpose of this study was to develop a simple seeding strategy with an optimal seeding duration...
May 22, 2018: Plastic and Reconstructive Surgery
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