keyword
MENU ▼
Read by QxMD icon Read
search

Adipose derived mesenchymal stem cells

keyword
https://www.readbyqxmd.com/read/28219809/thermoresponsive-polymer-modified-microfibers-for-cell-separations
#1
Kenichi Nagase, Yoichi Sakurada, Satoru Onizuka, Takanori Iwata, Masayuki Yamato, Naoya Takeda, Teruo Okano
Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells...
February 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28217409/muscle-derived-stem-cells-stimulate-muscle-myofiber-repair-and-counteract-fat-infiltration-in-a-diabetic-mouse-model-of-critical-limb-ischemia
#2
J Tsao, I Kovanecz, N Awadalla, R Gelfand, I Sinha-Hikim, R A White, N F Gonzalez-Cadavid
BACKGROUND: Critical Limb Ischemia (CLI) affects patients with Type 2 Diabetes (T2D) and obesity, with high risk of amputation and post-surgical mortality, and no effective medical treatment. Stem cell therapy, mainly with bone marrow mesenchymal, adipose derived, endothelial, hematopoietic, and umbilical cord stem cells, is promising in CLI mouse and rat models and is in clinical trials. Their general focus is on angiogenic repair, with no reports on the alleviation of necrosis, lipofibrosis, and myofiber regeneration in the ischemic muscle, or the use of Muscle Derived Stem Cells (MDSC) alone or in combination with pharmacological adjuvants, in the context of CLI in T2D...
December 2016: Journal of Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28213979/mmp-2-and-mmp-14-silencing-inhibits-vegfr2-cleavage-and-induces-the-differentiation-of-porcine-adipose-derived-mesenchymal-stem-cells-to-endothelial-cells
#3
Sami G Almalki, Yovani Llamas Valle, Devendra K Agrawal
The molecular mechanisms that control the ability of adipose-derived mesenchymal stem cells (AMSCs) to remodel three-dimensional extracellular matrix barriers during differentiation are not clearly understood. Herein, we studied the expression of matrix metalloproteinases (MMPs) during the differentiation of AMSCs to endothelial cells (ECs) in vitro. MSCs were isolated from porcine abdominal adipose tissue, and characterized by immunopositivity to CD44, CD90, CD105, and immunonegativity to CD14 and CD45. Plasticity of AMSCs was confirmed by multilineage differentiation...
February 18, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28210226/combination-of-collagen-based-scaffold-and-bioactive-factors-induces-adipose-derived-mesenchymal-stem-cells-chondrogenic-differentiation-in-vitro
#4
Giovanna Calabrese, Stefano Forte, Rosario Gulino, Francesco Cefalì, Elisa Figallo, Lucia Salvatorelli, Eugenia T Maniscalchi, Giuseppe Angelico, Rosalba Parenti, Massimo Gulisano, Lorenzo Memeo, Raffaella Giuffrida
Recently, multipotent mesenchymal stem cells (MSCs) have attracted much attention in the field of regenerative medicine due to their ability to give rise to different cell types, including chondrocytes. Damaged articular cartilage repair is one of the most challenging issues for regenerative medicine, due to the intrinsic limited capability of cartilage to heal because of its avascular nature. While surgical approaches like chondral autografts and allografts provide symptoms and function improvement only for a short period, MSC based stimulation therapies, like microfracture surgery or autologous matrix-induced chondrogenesis demonstrate to be more effective...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28209525/stromal-vascular-fraction-shows-robust-wound-healing-through-high-chemotactic-and-epithelialization-property
#5
Dong-Sic Chae, Seongho Han, Mina Son, Sung-Whan Kim
BACKGROUND: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated. AIMS: In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing. METHODS: Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA...
February 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28206643/in-vivo-exposure-to-inorganic-arsenic-alters-differentiation-specific-gene-expression-of-adipose-derived-mesenchymal-stem-stromal-cells-in-c57bl-6j-mouse-model
#6
Joseph J Shearer, Manoel Figueiredo Neto, C Samuel Umbaugh, Marxa L Figueiredo
No abstract text is available yet for this article.
February 16, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28205638/effects-of-mir-146a-on-the-osteogenesis-of-adipose-derived-mesenchymal-stem-cells-and-bone-regeneration
#7
Qing Xie, Wei Wei, Jing Ruan, Yi Ding, Ai Zhuang, Xiaoping Bi, Hao Sun, Ping Gu, Zi Wang, Xianqun Fan
Increasing evidence has indicated that bone morphogenetic protein 2 (BMP2) coordinates with microRNAs (miRNAs) to form intracellular networks regulating mesenchymal stem cells (MSCs) osteogenesis. This study aimed to identify specific miRNAs in rat adipose-derived mesenchymal stem cells (ADSCs) during BMP2-induced osteogenesis, we selected the most significantly down-regulated miRNA, miR-146a, to systematically investigate its role in regulating osteogenesis and bone regeneration. Overexpressing miR-146a notably repressed ADSC osteogenesis, whereas knocking down miR-146a greatly promoted this process...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28204811/isopsoralen-mediated-suppression-of-bone-marrow-adiposity-and-attenuation-of-the-adipogenic-commitment-of-bone-marrow-derived-mesenchymal-stem-cells
#8
Jian Wang, Sheng-Fa Li, Ting Wang, Chun-Han Sun, Liang Wang, Min-Jun Huang, Jian Chen, Shao-Wei Zheng, Nan Wang, Ying-Jun Zhang, Tian-Yu Chen
Osteoporosis (OP) increases the risk of bone fractures and other complications, and is thus a major clinical problem. In this study, we examined the effect of isopsoralen on the differentiation of bone-derived marrow mesenchymal stem cells (BMSCs) into osteoblasts and adipocytes, as well as bone formation under osteoporotic conditions. Primary femoral BMSCs isolated from C57BL/6 mice were used to evaluate the isopsoralen-mediated regulation of the expression of alkaline phosphatase (ALP), osteocalcin (OCN) and runt-related transcription factor 2 (RUNX2) during osteogenesis 2 weeks...
March 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28202074/exendin-4-in-combination-with-adipose-derived-stem-cells-promotes-angiogenesis-and-improves-diabetic-wound-healing
#9
Eunhui Seo, Jae Soo Lim, Jin-Bum Jun, Woohyuk Choi, In-Sun Hong, Hee-Sook Jun
BACKGROUND: Diminished wound healing is a major complication of diabetes mellitus and can lead to foot ulcers. However, there are limited therapeutic methods to treat this condition. Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor agonist, is known to have many beneficial effects on diabetes. In addition, mesenchymal stem cells are known to have wound healing effects. We investigated the effects of Ex-4 in combination with human adipose tissue-derived stem cells (ADSCs) on diabetic wound healing in a diabetic animal model...
February 15, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28201959/differentiation-potential-of-mesenchymal-stem-cells-derived-from-adipose-tissue-vs-bone-marrow-toward-annulus-fibrosus-cells-in-vitro
#10
Yifei Zhou, Xuqi Hu, Xuhao Zheng, Yaosen Wu, Naifeng Tian, Huazi Xu, Xiaolei Zhang
This study was to compare differentiation potential of bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) from Sprague-Dawley rats towards annulus fibrosus (AF)-like cells. Using coculture plates with 0.4μm pore size polyethylene terephthalate (PET) track-etched inserts (Corning,USA), MSCs and AF cells were cocultured with direct cell-to-cell contact up till 21 days. We evaluated the AF markers expression at the histological level and molecular level. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) results showed up regulation in AF marker gene expressions of both two MSCs after coculture...
February 13, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28197017/characterization-and-therapeutic-application-of-canine-adipose-mesenchymal-stem-cells-to-treat-elbow-osteoarthritis
#11
Éva Kriston-Pál, Ágnes Czibula, Zoltán Gyuris, Gyula Balka, Antal Seregi, Farkas Sükösd, Miklós Süth, Endre Kiss-Tóth, Lajos Haracska, Ferenc Uher, Éva Monostori
Visceral adipose tissue (AT) obtained from surgical waste during routine ovariectomies was used as a source for isolating canine mesenchymal stem cells (MSCs). As determined by cytofluorimetry, passage 2 cells expressed MSC markers CD44 and CD90 and were negative for lineage-specific markers CD34 and CD45. The cells differentiated toward osteogenic, adipogenic, and chondrogenic directions. With therapeutic aims, 30 dogs (39 joints) suffering from elbow dysplasia (ED) and osteoarthritis (OA) were intra-articularly transplanted with allogeneic MSCs suspended in 0...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28194905/a-proinflammatory-secretome-mediates-the-impaired-immunopotency-of-human-mesenchymal-stromal-cells-in-elderly-patients-with-atherosclerosis
#12
Özge Kizilay Mancini, Maximilien Lora, Dominique Shum-Tim, Stephanie Nadeau, Francis Rodier, Inés Colmegna
Inflammation plays a pivotal role in the initiation and progression of atherosclerosis (ATH). Due to their potent immunomodulatory properties, mesenchymal stromal cells (MSCs) are evaluated as therapeutic tools in ATH and other chronic inflammatory disorders. Aging reduces MSCs immunopotency potentially limiting their therapeutic utility. The mechanisms that mediate the effect of age on MSCs immune-regulatory function remain elusive and are the focus of this study. Human adipose tissue-derived MSCs were isolated from patients undergoing coronary artery bypass graft surgery...
February 14, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191762/periodontal-tissue-regeneration-using-syngeneic-adipose-derived-stromal-cells-in-a-mouse-model
#13
Mathieu Lemaitre, Paul Monsarrat, Vincent Blasco-Baque, Pascale Loubières, Rémy Burcelin, Louis Casteilla, Valérie Planat-Bénard, Philippe Kémoun
Current treatment of periodontitis is still associated with a high degree of variability in clinical outcomes. Recent advances in regenerative medicine by mesenchymal cells, including adipose stromal cells (ASC) have paved the way to improved periodontal regeneration (PD) but little is known about the biological processes involved. Here, we aimed to use syngeneic ASCs for periodontal regeneration in a new, relevant, bacteria-induced periodontitis model in mice. Periodontal defects were induced in female C57BL6/J mice by oral gavage with periodontal pathogens...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28186708/mesenchymal-stem-cell-derived-extracellular-vesicles-as-mediators-of-anti-inflammatory-effects-endorsement-of-macrophage-polarization
#14
Claudia Lo Sicco, Daniele Reverberi, Carolina Balbi, Valentina Ulivi, Elisa Principi, Luisa Pascucci, Pamela Becherini, Maria Carla Bosco, Luigi Varesio, Chiara Franzin, Michela Pozzobon, Ranieri Cancedda, Roberta Tasso
Mesenchymal Stem Cells (MSCs) are effective therapeutic agents enhancing the repair of injured tissues mostly through their paracrine activity. Increasing evidences show that besides the secretion of soluble molecules, the release of extracellular vesicles (EVs) represents an alternative mechanism adopted by MSCs. Since macrophages are essential contributors toward the resolution of inflammation, which has emerged as a finely orchestrated process, the aim of the present study was to carry out a detailed characterization of EVs released by human adipose derived-MSCs to investigate their involvement as modulators of MSC anti-inflammatory effects inducing macrophage polarization...
January 31, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28186687/identification-of-potential-plasma-microrna-stratification-biomarkers-for-response-to-allogeneic-adipose-derived-mesenchymal-stem-cells-in-rheumatoid-arthritis
#15
David J Mallinson, Donald R Dunbar, Susan Ridha, Elizabeth R Sutton, Olga De la Rosa, Wilfried Dalemans, Eleuterio Lombardo
The ability to identify and stratify patients that will respond to specific therapies has been transformational in a number of disease areas, particularly oncology. It is anticipated that this will also be the case for cell-based therapies, particularly in complex and heterogeneous diseases such as rheumatoid arthritis (RA). Recently, clinical results with expanded allogenic adipose-derived mesenchymal stem cells (eASCs) have indicated clinical efficacy in highly refractory RA patients. In this study, we set out to determine if circulating microRNAs (miRNAs) could be identified as potential biomarkers associated with response to eASCs in these RA patients...
January 3, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28174671/evaluation-of-serum-free-xeno-free-cryopreservation-solutions-for-human-adipose-derived-mesenchymal-stem-cells
#16
Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free)...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28173869/characteristics-of-human-adipose-derived-stem-cells-in-scleroderma-in-comparison-to-sex-and-age-matched-normal-controls-implications-for-regenerative-medicine
#17
Michelle Griffin, Caroline M Ryan, Omar Pathan, David Abraham, Christopher P Denton, Peter E M Butler
BACKGROUND: Adipose-derived stem cells (ADSCs) are emerging as an alternative stem cell source for cell-based therapies. Recent data suggest that autologous ADSC-enriched micrografting improves the effects of facial involvement in systemic sclerosis (SSc). We have extensively characterised ADSCs from SSc patients and compared their phenotype and function to healthy age- and sex-matched control ADSCs. METHODS: ADSCs were isolated and characterised from a cohort of six SSc patients (ADSC-SSc) and were compared to six healthy age- and sex-matched controls (ADSC-N)...
February 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28172411/op009-long-term-efficacy-and-safety-of-cx601-allogeneic-expanded-adipose-derived-mesenchymal-stem-cells-for-complex-perianal-fistulas-in-crohn-s-disease-52-week-results-of-a-phase-iii-randomised-controlled-trial
#18
J Panes, D García-Olmo, G Van Assche, J-F Colombel, W Reinisch, D C Baumgart, M Nachury, M Ferrante, L Kazemi-Shirazi, J C Grimaud, F de la Portilla, E Goldin, M P Richard, M C Diez, S Danese
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28170197/enhanced-cardioprotection-by-human-endometrium-mesenchymal-stem-cells-driven-by-exosomal-microrna-21
#19
Kan Wang, Zhi Jiang, Keith A Webster, Jinghai Chen, Hengxun Hu, Yu Zhou, Jing Zhao, Lihan Wang, Yingchao Wang, Zhiwei Zhong, Cheng Ni, Qingju Li, Charlie Xiang, Ling Zhang, Rongrong Wu, Wei Zhu, Hong Yu, Xinyang Hu, Jian'an Wang
Our group recently reported positive therapeutic benefit of human endometrium-derived mesenchymal stem cells (EnMSCs) delivered to infarcted rat myocardium, an effect that correlated with enhanced secretion of protective cytokines and growth factors compared with parallel cultures of human bone marrow MSCs (BMMSCs). To define more precisely the molecular mechanisms of EnMSC therapy, in the present study, we assessed in parallel the paracrine and therapeutic properties of MSCs derived from endometrium, bone marrow, and adipose tissues in a rat model of myocardial infarction (MI)...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170177/pluripotent-nontumorigenic-adipose-tissue-derived-muse-cells-have-immunomodulatory-capacity-mediated-by-transforming-growth-factor-%C3%AE-1
#20
María L Gimeno, Florencia Fuertes, Andres E Barcala Tabarrozzi, Alejandra I Attorressi, Rodolfo Cucchiani, Luis Corrales, Talita C Oliveira, Mari C Sogayar, Leticia Labriola, Ricardo A Dewey, Marcelo J Perone
Adult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates. Muse-AT cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods...
January 2017: Stem Cells Translational Medicine
keyword
keyword
94889
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"