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Adipose derived mesenchymal stem cells

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https://www.readbyqxmd.com/read/29453764/comparative-analysis-of-human-ucb-and-adipose-tissue-derived-mesenchymal-stem-cells-for-their-differentiation-potential-into-brown-and-white-adipocytes
#1
Afrooz Rashnonejad, Gulinnaz Ercan, Cumhur Gunduz, Ali Akdemir, Yigit Ozer Tiftikcioglu
The differentiation potential of umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) into brown and white adipocytes in comparison to Adipose tissue derived MSCs (AD-MSCs) were investigated in order to characterize their potency for future cell therapies. MSCs were isolated from ten UCB samples and six liposuction materials. MSCs were differentiated into white and brown adipocytes after characterization by flow cytometry. Differentiated adipocytes were stained with Oil Red O and hematoxylin/eosin...
February 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29451213/bone-marrow-derived-mesenchymal-stem-cells-versus-adipose-derived-mesenchymal-stem-cells-for-peripheral-nerve-regeneration
#2
Marcela Fernandes, Sandra Gomes Valente, Rodrigo Guerra Sabongi, João Baptista Gomes Dos Santos, Vilnei Mattioli Leite, Henning Ulrich, Arthur Andrade Nery, Maria José da Silva Fernandes
Studies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29448017/fetal-bovine-serum-free-cryopreservation-methods-for-clinical-banking-of-human-adipose-derived-stem-cells
#3
Seah Park, Dong Ryul Lee, Ji Sun Nam, Chul Woo Ahn, Haekwon Kim
The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. Here, we investigated if human serum albumin (HSA), human serum (HS), or knockout serum replacement (KSR) can replace FBS for the cryopreservation of MSCs. In addition, we examined the characteristics of MSCs after multiple rounds of cryopreservation. Human adipose-derived stem cells (ASCs) cryopreserved with three FBS replacements, 9% HSA, 90% HS, or 90% KSR, in combination with 10% dimethyl sulfoxide (Me 2 SO) maintained stem cell properties including growth, immunophenotypes, gene expression patterns, and the potential to differentiate into adipogenic, osteogenic, and chondrogenic lineages, similar to ASCs frozen with FBS...
February 12, 2018: Cryobiology
https://www.readbyqxmd.com/read/29446551/targeting-murine-mesenchymal-stem-cells-to-kidney-injury-molecule-1-improves-their-therapeutic-efficacy-in-chronic-ischemic-kidney-injury
#4
Xiangyu Zou, Kai Jiang, Amrutesh S Puranik, Kyra L Jordan, Hui Tang, Xiangyang Zhu, Lilach O Lerman
Mesenchymal stem cells (MSC) have been experimentally used for kidney repair, but modest retention limits their efficacy. Cell-surface coating allows modulating MSC homing and interaction with target cells. We coated mouse adipose tissue-derived MSC with antibodies directed against kidney injury molecule-1 (ab-KIM1), which is upregulated in injured kidneys, and tested the hypothesis that this would enhance their therapeutic effects in ischemic kidney injury. Untreated MSC, ab-KIM1-coated MSC (KIM-MSC), or vehicle, were injected systemically into the carotid artery of 2-kidneys, 1-clip mice 2 weeks after surgery...
February 15, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29445958/histopathological-and-behavioral-assessments-of-aging-effects-on-stem-cell-transplants-in-an-experimental-traumatic-brain-injury
#5
Jea-Young Lee, Roger Lin, Hung Nguyen, M Grant Liska, Trenton Lippert, Yuji Kaneko, Cesar V Borlongan
Traumatic brain injury (TBI) displays cognitive and motor symptoms following the initial injury which can be exacerbated by secondary cell death. Aging contributes significantly to the morbidity of TBI, with higher rates of negative neurological and behaviors outcomes. In the recent study, young and aged animals were injected intravenously with human adipose-derived mesenchymal stem cells (hADSCs) (Tx), conditioned media (CM), or vehicle (unconditioned media) following TBI. The beneficial effects of hADSCs were analyzed using various molecular and behavioral techniques...
February 15, 2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29443020/autologous-microfractured-and-purified-adipose-tissue-for-arthroscopic-management-of-osteochondral-lesions-of-the-talus
#6
Riccardo D'Ambrosi, Cristian Indino, Camilla Maccario, Luigi Manzi, Federico Giuseppe Usuelli
In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several studies have focused their attention on mesenchymal stem cells derived from adipose tissue. Adipose-derived stem cells (ADSCs) exhibit morphological characteristics and properties similar to other mesenchymal cells, and are able to differentiate into several cellular lines. Moreover, these cells are also widely available in the subcutaneous tissue, representing 10 - 30% of the normal body weight, with a concentration of 5,000 cells per gram of tissue...
January 23, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29442706/human-adipose-derived-stem-cells-and-osteoblasts-interaction-with-fe-cr-nb-b-magnetic-nanoparticles
#7
Luminita Simion Labusca, Dumitru-Daniel Herea, Ecaterina Radu, Camelia Danceanu, Horia Chiriac, Nicoleta Lupu
The use of materials at nanoscale is currently of increasing interest for life sciences and medicine. Magnetic nanoparticles (MNPs) are under scrutiny for a large array of applications in nanomedicine as diagnostic and therapeutic tools. Proprietary Fe-Cr-Nb-B MNPs display heating properties that recommends them as potent agents for delivery of local hyperthermia for the treatment of solid tumours. Stem cell mediated delivery represents a safe and accurate modality to target remote or metastatic tumour sites...
July 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442525/comparative-analysis-of-mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-dental-pulp-as-sources-of-cell-therapy-for-zone-of-stasis-burns
#8
Ozan Luay Abbas, Orhan Özatik, Zeynep Burçin Gönen, Serdal Öğüt, Fikriye Yasemin Özatik, Hasan Salkın, Ahmet Musmul
INTRODUCTION: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations...
February 14, 2018: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/29441951/lncrna-hulc-promotes-epithelial-and-smooth-muscle-like-differentiation-of-adipose-derived-stem-cells-by-upregulation-of-bmp9
#9
Yongwei Li, Zhengfei Shan, Bin Yang, Diandong Yang, Changping Men, Yuanshan Cui, Jitao Wu
AIMS: Adipose-derived stem cells (ADSCs), a source of mesenchymal stem cells, are able to differentiate into numerous cell lineages, including epithelial and smooth muscle cells. The use of ADSCs in tissue engineering technology has become the most promising therapeutic approach for urethral reconstruction. This study aimed to explore the effect of lncRNA highly upregulated in liver cancer (HULC) on the induction of ADSCs to differentiate into epithelial and smooth-muscle-like cells. METHODS: ADSCs were isolated from a male dog, and the expression of HULC in ADSCs was overexpressed by transfection with HULC expressing vector lentivirus...
January 2, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29441709/multifunctional-cell-instructive-silk-bioactive-glass-composite-reinforced-scaffolds-toward-osteoinductive-proangiogenic-and-resorbable-bone-grafts
#10
Joseph Christakiran Moses, Samit Kumar Nandi, Biman B Mandal
The successful regeneration of large volume bone defects necessitates the use of proangiogenic and resorbable scaffolding matrix. Impaired and slow ingrowth of host vasculature within implanted grafts greatly compromises its effective osseointegration. By addressing this, it is demonstrated that the use of copper doped bioactive glass functionalizes silk microfiber reinforcements to improve the physicochemical and osteoinductive properties of two silk scaffolding matrices (mulberry Bombyx mori and non-mulberry Antheraea assama) employed in the study...
February 14, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29439287/demonstration-of-improved-tissue-integration-and-angiogenesis-with-an-elastic-estradiol-releasing-polyurethane-material-designed-for-use-in-pelvic-floor-repair
#11
Sarah Shafaat, Naside Mangir, Sabiniano R Regureos, Christopher R Chapple, Sheila MacNeil
AIMS: Pelvic organ prolapse and stress urinary incontinence affect 40-50% of postmenopausal women worldwide. Polypropylene meshes have been extensively used for the surgical intervention of these disorders; however, these meshes can lead to severe complications in some patients. The need for synthetic materials more suited for use in pelvic floor repair is widely accepted. This study aims to develop an electrospun 17-β-estradiol releasing polyurethane (PU) scaffold that not only provides the appropriate mechanical support but can also stimulate new extracellular matrix (ECM) production and angiogenesis...
February 13, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29434773/human-adipose-derived-mesenchymal-stem-cell-conditioned-media-suppresses-inflammatory-bone-loss-in-a-lipopolysaccharide-induced-murine-model
#12
Yu Li, Xin Gao, Jinbing Wang
Conditioned media (CM) from mesenchymal stem cells (MSCs) contains various cytokines, growth factors and microRNAs, which may serve important roles in modulating the inflammatory process. However, the effect of MSC-CM on inflammatory bone loss remains unknown. The present study investigated the effects of conditioned media from human adipose-derived mesenchymal stem cells (AMSC-CM) on the prevention of lipopolysaccharide (LPS)-mediated bone loss in mice. To investigate the underlying mechanisms of this effect, the effects of AMSC-CM on serum levels of inflammation-associated cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 and IL-10] in LPS-treated mice, in addition to their mRNA expression in LPS-treated macrophages, was investigated...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29434727/differentiation-of-rat-adipose-derived-mesenchymal-stem-cells-into-corneal-like-epithelial-cells-driven-by-pax6
#13
Jing Sun, Wei-Hua Liu, Feng-Mei Deng, Yong-Hui Luo, Ke Wen, Hong Zhang, Hai-Rong Liu, Jiang Wu, Bing-Yin Su, Yi-Lun Liu
Corneal integrity, transparency and vision acuity are maintained by corneal epithelial cells (CECs), which are continuously renewed by corneal limbal stem cells (LSCs). Deficiency of CECs and/or LSCs is associated with numerous ocular diseases. Paired box (PAX)6 is an eye development-associated transcription factor that is necessary for cell fate determination and differentiation of LSCs and CECs. In the present study, the PAX6 gene was introduced into adipose-derived rat mesenchymal stem cells (ADMSCs) to investigate whether PAX6-transfected cells were able to transdifferentiate into corneal-like epithelial cells and to further verify whether the cells were suitable as a cell source for corneal transplantation...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29434684/intraglandular-transplantation-of-adipose-derived-stem-cells-combined-with-platelet-rich-fibrin-extract-for-the-treatment-of-irradiation-induced-salivary-gland-damage
#14
Zhifa Wang, Hongyan Xing, Hanqing Hu, Taiqiang Dai, Yan Wang, Zhijin Li, Ran An, Haiyan Xu, Yanpu Liu, Bin Liu
The aim of the present study was to determine the effect of adipose-derived mesenchymal stem cells (ADSCs) combined with heterologous platelet-rich fibrin extract (PRFe) on irradiation-induced salivary gland (SG) damage. ADSCs were isolated from C3H mice, whereas PRFe was obtained from New Zealand rabbits. Twelve weeks post irradiation, the ADSCs or PRFe or their combination were transplanted into the submandibular glands of C3H mice with irradiation-induced damage. The salivary flow rate (SFR) was determined and histopathological analysis was performed at 12 weeks post transplantation...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29433579/the-role-of-fibrinolysis-inhibition-in-engineered-vascular-networks-derived-from-endothelial-cells-and-adipose-derived-stem-cells
#15
Severin Mühleder, Karoline Pill, Mira Schaupper, Krystyna Labuda, Eleni Priglinger, Pablo Hofbauer, Verena Charwat, Uwe Marx, Heinz Redl, Wolfgang Holnthoner
BACKGROUND: Co-cultures of endothelial cells with mesenchymal stem cells currently represent one of the most promising approaches in providing oxygen and nutrient supply for microvascular tissue engineering. Still, to translate this model into clinics several in vitro parameters including growth medium and scaffold degradation need to be fine-tuned. METHODS: We recently described the co-culture of adipose-derived stem cells with endothelial cells in fibrin, resulting in capillary formation in vitro as well as their perfusion in vivo...
February 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29431030/angiogenic-activity-of-human-adipose-derived-mesenchymal-stem-cells-under-simulated-microgravity
#16
Andrey Ratushnyy, Maria Ezdakova, Danila Yakubets, Ludmila Buravkova
Multipotent mesenchymal stem/stromal cells (MSCs) are strongly involved in tissue homeostasis mainly through paracrine regulation. Here we examined the influence of simulated microgravity on the angiogenic potential of adipose-derived MSCs (ASCs). The conditioned medium (CM) from RPM-exposed ASCs stimulated the formation of vessel network in ovo, endothelial cell (EC) capillary-like network ex ovo and non-directed EC migration in vitro. These effects were driven by alteration of both angiogenesis-related gene and protein expression...
February 12, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29429756/corrigendum-to-sdf-1-improves-wound-healing-ability-of-glucocorticoid-treated-adipose-tissue-derived-mesenchymal-stem-cells-biochem-biophys-res-commun-493-2-2017-1010-1017
#17
Toshiki Kato, Vuong Cat Khanh, Kazutoshi Sato, Kosuke Takeuchi, Erica Carolina, Toshiharu Yamashita, Hisashi Sugaya, Tomokazu Yoshioka, Hajime Mishima, Osamu Ohneda
No abstract text is available yet for this article.
February 8, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29429509/physical-stimulation-and-scaffold-composition-efficiently-support-osteogenic-differentiation-of-mesenchymal-stem-cells
#18
Sepide Heydari Asl, Hoorieh Hosseinpoor, Kazem Parivar, Nasim Hayati Roodbari, Hana Hanaee-Ahvaz
BACKGROUND: Despite significant achievements in the field of tissue engineering, simplification and improvement of the existing protocols are of great importance. The use of complex differentiation media, due to the presence of multiple factors, may have some undesired effects on cell health and functions. Thus, minimizing the number of involved factors, while maintaining the differentiation efficiency, provides less costly and controllable conditions. Adipose-derived Mesenchymal stem cells (ASCs), the adult stem cells present in adipose tissue, can be a suitable source of stem cells due to abundant and ease of access...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429450/human-amnion-derived-mesenchymal-stem-cells-induced-osteogenesis-and-angiogenesis-in-human-adipose-derived-stem-cells-via-erk1-2-mapk-signaling-pathway
#19
Yuli Wang, Xichen Chen, Ying Yin, Song Li
Mesenchymal stem cells (MSCs) have shown great potential in treating bone deficiency. Human adipose-derived stem cells (HASCs) are multipotent progenitor cells with multi-lineage differentiation potential. Human amnion-derived mesenchymal stem cells (HAMSCs) are capable of promoting osteogenic differentiation of MSCs. In this study, we investigated the effect of HAMSCs on HASCs by a transwell co-culture system. HAMSCs promoted proliferation, osteogenic differentiation, angiogenic potential and adiponectin (APN) secretion of HASCs...
February 12, 2018: BMB Reports
https://www.readbyqxmd.com/read/29428734/co-transplantation-of-exosomes-derived-from-hypoxia-preconditioned-adipose-mesenchymal-stem-cells-promotes-neovascularization-and-graft-survival-in-fat-grafting
#20
Yu-di Han, Yun Bai, Xin-Long Yan, Jing Ren, Quan Zeng, Xiao-Dong Li, Xue-Tao Pei, Yan Han
BACKGROUND: Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proangiogenic potential of stem cell. This study aimed to investigate the effect of ADSC-derived exosomes (ADSC-Exos) on the survival in fat grafting. METHODS: A nude mouse model of subcutaneous fat grafting was adopted. Hypoxic preconditioned ADSC-Exos and ADSC-Exos were injected around the grafted tissue. The fat graft sample was weighed and examined by hematoxylin and eosin (H&E) staining and immunohistochemistry...
February 8, 2018: Biochemical and Biophysical Research Communications
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