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Adipose derived mesenchymal stem cells

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https://www.readbyqxmd.com/read/28078245/the-effects-of-cinnamaldehyde-and-eugenol-on-human-adipose-derived-mesenchymal-stem-cells-viability-growth-and-differentiation-a-cheminformatics-and-in-vitro-study
#1
Abdorrahim Absalan, Seyed Alireza Mesbah-Namin, Taki Tiraihi, Taher Taheri
OBJECTIVE: The aim of this study was to estimate the cheminformatics and qualitative structure-activity relationship (QSAR) of cinnamaldehyde and eugenol. The effects of cinnamaldehyde and eugenol on the viability, doubling time and adipogenic or osteogenic differentiations of human adipose-derived mesenchymal stem cells (hASCs) were also investigated. MATERIALS AND METHODS: QSAR and toxicity indices of cinnamaldehyde and eugenol were evaluated using cheminformatics tools including Toxtree and Toxicity Estimation Software Tool (T...
November 2016: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28077523/autologous-adipose-derived-mesenchymal-stem-cells-improve-healing-of-coiled-experimental-saccular-aneurysms-an-angiographic-and-histopathological-study
#2
Aymeric Rouchaud, Waleed Brinjikji, Daying Dai, Yong-Hong Ding, Tina Gunderson, Dana Schroeder, Laurent Spelle, David F Kallmes, Ramanathan Kadirvel
PURPOSE: Long-term occlusion of coiled aneurysms frequently fails, probably because of poor intrasaccular healing and inadequate endothelialization across the aneurysm neck. The purpose of this study was to determine if attachment of autologous mesenchymal stem cells (MSCs) to platinum coils would improve the healing response in an elastase-induced aneurysm model in rabbits. MATERIALS AND METHODS: With approval from the institutional animal care and use committee, aneurysms were created in rabbits and embolized with control platinum coils (Axium; Medtronic) (n=6) or coils seeded ex vivo with autologous adipose-tissue MSCs (n=7)...
January 11, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28071738/purification-and-differentiation-of-human-adipose-derived-stem-cells-by-membrane-filtration-and-membrane-migration-methods
#3
Hong Reng Lin, Chao-Wen Heish, Cheng-Hui Liu, Saradaprasan Muduli, Hsing-Fen Li, Akon Higuchi, S Suresh Kumar, Abdullah A Alarfaj, Murugan A Munusamy, Shih-Tien Hsu, Da-Chung Chen, Giovanni Benelli, Kadarkarai Murugan, Nai-Chen Cheng, Han-Chow Wang, Gwo-Jang Wu
Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28070458/characterization-of-adipose-tissue-macrophages-and-adipose-derived-stem-cells-in-critical-wounds
#4
Bong-Sung Kim, Pathricia V Tilstam, Katrin Springenberg-Jung, Arne Hendrick Boecker, Corinna Schmitz, Daniel Heinrichs, Soo Seok Hwang, Jan Philipp Stromps, Bergita Ganse, Ruedger Kopp, Matthias Knobe, Juergen Bernhagen, Norbert Pallua, Richard Bucala
BACKGROUND: Subcutaneous adipose tissue is a rich source of adipose tissue macrophages and adipose-derived stem cells which both play a key role in wound repair. While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers. METHODS: In the present study, we have investigated the expression of common macrophage polarization and stem cell markers in acutely inflamed adipose tissue...
2017: PeerJ
https://www.readbyqxmd.com/read/28070061/injectable-alginate-microencapsulated-canine-adipose-tissue-derived-mesenchymal-stem-cells-for-enhanced-viable-cell-retention
#5
Eunji Koh, Yun Chan Jung, Heung-Myong Woo, Byung-Jae Kang
The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro...
January 6, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28056548/l-carnitine-contributes-to-enhancement-of-neurogenesis-from-mesenchymal-stem-cells-through-wnt-%C3%AE-catenin-and-pka-pathway
#6
Ezzatollah Fathi, Raheleh Farahzadi, Hojjatollah N Charoudeh
The identification of factors capable of enhancing neurogenesis has great potential for cellular therapies in neurodegenerative diseases. Multiple studies have shown the neuroprotective effects of L-carnitine (LC). This study determined whether neuronal differentiation of rat adipose tissue-derived mesenchymal stem cells (ADSCs) can be activated by LC. In this study, protein kinase A (PKA) and Wnt/β-catenin pathways were detected to show if this activation was due to these pathways. The expression of LC-induced neurogenesis markers in ADSCs was characterized using real-time PCR...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28055225/overexpression-of-prox1-induces-the-differentiation-of-human-adipose-derived-stem-cells-into-lymphatic-endothelial-like-cells-in-vitro
#7
Jingcheng Deng, Tingting Dai, Yiyu Sun, Qi Zhang, Zhaohua Jiang, Shengli Li, Weigang Cao
Constant levels of homeobox transcription factor Prox1 expression are required throughout the life of lymphatic endothelial cells (LECs) to maintain their differentiated identity. Recent studies have demonstrated that using human LECs for cell transplantation therapy may improve secondary lymphedema in a nude rat model. However, the application is currently limited by the low yield of LECs. In this study, Prox1 was overexpressed in human adipose tissue-derived stem cells (hADSCs) by using the transfection of lentiviral vectors to induce the differentiation of hADSCs to LECs...
January 5, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28052725/paracrine-activity-from-adipose-derived-stem-cells-on-in-vitro-wound-healing-in-human-tympanic-membrane-keratinocytes
#8
Huan Ting Ong, Sharon Redmond, Robert Marano, Marcus Atlas, Magnus von Unge, Peder Aabel, Rodney J Dilley
Stem cell therapies for tympanic membrane repair have shown initial experimental success using mesenchymal stem cells in rat models to promote healing, however the mechanisms providing this benefit are not known. We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSC) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalised human keratinocytes (HaCat). ADSC conditioned media (CMADSC) were assessed for paracrine activity on keratinocyte proliferation and migration, with hypoxic conditions for ADSC culture used to generate contrasting effects on cytokine gene expression...
January 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28051244/moringa-oleifera-lam-improves-lipid-metabolism-during-adipogenic-differentiation-of-human-stem-cells
#9
I Barbagallo, L Vanella, A Distefano, D Nicolosi, A Maravigna, G Lazzarino, M Di Rosa, D Tibullo, R Acquaviva, G Li Volti
OBJECTIVE: Moringa oleifera Lam., a multipurpose tree, is used traditionally for its nutritional and medicinal properties. It has been used for the treatment of a variety of conditions, including inflammation, cancer and metabolic disorders. MATERIALS AND METHODS: We investigated the effect of Moringa oleifera Lam. on adipogenic differentiation of human adipose-derived mesenchymal stem cells and its impact on lipid metabolism and cellular antioxidant systems. RESULTS: We showed that Moringa oleifera Lam...
December 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28043752/fibrous-synovium-releases-higher-numbers-of-mesenchymal-stem-cells-than-adipose-synovium-in-a-suspended-synovium-culture-model
#10
Kenta Katagiri, Yu Matsukura, Takeshi Muneta, Nobutake Ozeki, Mitsuru Mizuno, Hisako Katano, Ichiro Sekiya
PURPOSE: To develop an in vitro model, the "suspended synovium culture model," to demonstrate the mobilization of mesenchymal stem cells (MSCs) from the synovium into a noncontacted culture dish through culture medium. In addition, to examine which synovium, fibrous synovium or adipose synovium, released more MSCs in the knee with osteoarthritis. METHODS: Human synovial tissue was harvested during total knee arthroplasty from knee joints of 34 patients with osteoarthritis (28 patients: only fibrous synovium, 6 patients: fibrous and adipose synovium)...
December 30, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28041853/stem-cells-in-male-sexual-dysfunction-are-we-getting-somewhere
#11
REVIEW
Mohammad Ayodhia Soebadi, Uros Milenkovic, Emmanuel Weyne, Fabio Castiglione, Maarten Albersen
INTRODUCTION: Stem cells for sexual disorders are steadily being introduced into clinical trials. Two conditions of importance are the main target for this line of treatment, especially when regarding the wide array of translational and basic science highlighting the potential advantages of regenerative therapy: erectile dysfunction (ED) and more recently Peyronie disease (PD). Cellular therapy offers a treatment modality that might reverse disease progression. It would be used in a curative setting, in contrast to other pharmaceutical agents that are currently available...
December 29, 2016: Sexual Medicine Reviews
https://www.readbyqxmd.com/read/28039611/alternative-new-mesenchymal-stem-cell-source-exerts-tumor-tropism-through-alcam-and-n-cadherin-via-regulation-of-microrna-192-and-218
#12
Ran Kim, Sang In Park, Chang Youn Lee, Jihyun Lee, Pilseog Kim, Sekyung Oh, Hojin Lee, Min Young Lee, Jongmin Kim, Yong-An Chung, Ki-Chul Hwang, Lee-So Maeng, Woochul Chang
Gliomas are the most common type of malignant primary brain tumors. Some treatments of gliomas exist, but they are rarely curative. Mesenchymal stem cells (MSCs) are emerging as potential modes of targeted cancer therapy owing to their capacity for homing toward tumor sites. It has been proposed that MSCs derived from various sources, such as bone marrow, adipose tissue and umbilical cord blood, can be used as cell-based therapy for brain tumors. Here, MSCs obtained from the synovial fluid of osteoarthritis or rheumatoid arthritis patients were investigated as therapeutic candidates...
December 30, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28039550/application-of-a-three-dimensional-graft-of-autologous-osteodifferentiated-adipose-stem-cells-in-patients-undergoing-minimally-invasive-transforaminal-lumbar-interbody-fusion-clinical-proof-of-concept
#13
E Fomekong, D Dufrane, B Vande Berg, W André, N Aouassar, S Veriter, C Raftopoulos
BACKGROUND: The authors applied a scaffold-free osteogenic three-dimensional (3D) graft made of adipose-derived mesenchymal stem cells (AMSCs) in patients undergoing minimally invasive transforaminal lumbar interbody fusion (MI-TLIF). METHODS: Three patients (two patients and one patient with 1 and 2 levels, respectively) with degenerative spondylolisthesis underwent MI-TLIF with 3D graft made of AMSCs. To obtain the AMSCs, fatty tissue was collected from the abdomen by lipoaspiration and differentiated afterwards in our Cell/Tissue bank...
December 30, 2016: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28035654/seven-days-post-injury-fate-and-effects-of-genetically-labelled-adipose-derived-mesenchymal-cells-on-a-rat-traumatic-brain-injury-experimental-model
#14
Ioanna Dori, Spyros Petrakis, Aggeliki Giannakopoulou, Chryssa Bekiari, Ioannis Grivas, Evangelia K Siska, Georgios Koliakos, Georgios C Papadopoulos
Mesenchymal stromal cells (MSC) have been suggested to have beneficial effects on animal models of traumatic brain injury (TBI), owing to their neurotrophic and immunomodulatory properties. Adipose tissue-derived stromal cells (ASCs) are multipotent MSC that can be harvested with minimally invasive methods, show a high proliferative capacity, low immunogenicity if allogeneic, and can be used in autologous or heterologous settings. In the present study ASCs were genetically labelled using the Sleeping Beauty transposon to express the fluorescent protein Venus...
December 30, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/28035603/reovirus-safety-study-for-proliferation-and-differentiation-of-human-adipose-derived-mesenchymal-stem-cells
#15
Jeong-Soo Park, Manbok Kim
Naturally occurring reoviruses are live replication-proficient viruses specifically infecting human cancer cells while sparing the normal counterparts. Stem cells can be highly susceptible to viral infection due to their innate high proliferation potential and other active signaling pathways of cells that might be involved in viral tropism. In the previous study, we showed that reoviruses could adversely affect murine embryonic stem cells' integrity in vitro and in vivo. Oncolytic viruses, delivered systemically face many hurdles that also impede their localization and infection of, metastatic tumors, due to a variety of immune and physical barriers...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28035134/upregulation-of-cc-chemokine-receptor-7-ccr7-enables-migration-of-xenogeneic-human-adipose-derived-mesenchymal-stem-cells-to-rat-secondary-lymphoid-organs
#16
Tian Ma, Shao-Liang Luan, Hong Huang, Xing-Kun Sun, Yan-Mei Yang, Hui Zhang, Wei-Dong Han, Hong Li, Yan Han
BACKGROUND CC chemokine receptor 7 (CCR7) expression is vital for cell migration to secondary lymphoid organs (SLOs). Our previous work showed that inducing CCR7 expression enabled syngeneic mesenchymal stem cells (MSCs) to migrate into SLOs, resulting in enhanced immunosuppressive performance in mice. Given that human adipose-derived stem cells (hASCs) are widely used in clinical therapy, we further investigated whether upregulation of CCR7 enables xenogeneic hASCs to migrate to rat SLOs. MATERIAL AND METHODS hASCs rarely express CCR7; therefore, hASCs were transfected with lentivirus encoding rat CCR7 (rCCR7) plus green fluorescence protein (GFP) or GFP alone...
December 30, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28034832/proteasome-activation-enhances-stemness-and-lifespan-of-human-mesenchymal-stem-cells
#17
Marianna Kapetanou, Niki Chondrogianni, Spyros Petrakis, George Koliakos, Efstathios S Gonos
The age-associated decline of adult stem cell function contributes to the physiological failure of homeostasis during aging. The proteasome plays a key role in the maintenance of proteostasis and its failure is associated with various biological phenomena including senescence and aging. Although stem cell biology has attracted intense attention, the role of proteasome in stemness and its age-dependent deterioration remains largely unclear. By employing both Wharton's-Jelly- and Adipose-derived human adult mesenchymal stem cells (hMSCs), we reveal a significant age-related decline in proteasome content and peptidase activities, accompanied by alterations of proteasomal complexes...
December 27, 2016: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28029762/prevascularization-of-decellularized-porcine-myocardial-slice-for-cardiac-tissue-engineering
#18
Pawan Kc, Mickey Shah, Jun Liao, Ge Zhang
Prevacularization strategies have been implemented in tissue engineering to generate microvasculature networks within a scaffold prior to implantation. Prevascularizing scaffolds will shorten the time of functional vascular perfusion with host upon implantation. In this study, we explored key variables affecting the interaction between decellularized porcine myocardium slices (dPMSs) and reseeded stem cells toward the fabrication of prevascularized cardiac tissue. Our results demonstrated that dPMS supports attachment of human mesenchymal stem cells (hMSCs) and rat adipose derived stem cells (rASCs) with high viability...
January 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28018432/adipose-derived-mesenchymal-stem-cells-viability-and-differentiating-features-for-orthopaedic-reparative-applications-banking-of-adipose-tissue
#19
Ilaria Roato, Daniela Alotto, Dimas Carolina Belisario, Stefania Casarin, Mara Fumagalli, Irene Cambieri, Raimondo Piana, Maurizio Stella, Riccardo Ferracini, Carlotta Castagnoli
Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious...
2016: Stem Cells International
https://www.readbyqxmd.com/read/28018212/mitochondrial-functional-changes-characterization-in-young-and-senescent-human-adipose-derived-mscs
#20
Bernd R Stab, Laura Martinez, Adriana Grismaldo, Alejandra Lerma, María L Gutiérrez, Luis A Barrera, Jhon J Sutachan, Sonia L Albarracín
Mitochondria are highly dynamic organelles that in response to the cell's bio-energetic state continuously undergo structural remodeling fission and fusion processes. This mitochondrial dynamic activity has been implicated in cell cycle, autophagy, and age-related diseases. Adult tissue-derived mesenchymal stromal/stem cells present a therapeutic potential. However, to obtain an adequate mesenchymal stromal/stem cell number for clinical use, extensive in vitro expansion is required. Unfortunately, these cells undergo replicative senescence rapidly by mechanisms that are not well understood...
2016: Frontiers in Aging Neuroscience
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