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Adipose derived mesenchymal stem cells

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https://www.readbyqxmd.com/read/29227527/m2-muscarinic-receptor-activation-inhibits-cell-proliferation-and-migration-of-rat-adipose-mesenchymal-stem-cells
#1
Roberta Piovesana, Simona Melfi, Mario Fiore, Valerio Yi, Ada Maria Tata
Mesenchymal stem cells (MSCs), also known as stromal mesenchymal stem cells, are multipotent cells, which can be found in many tissues and organs as bone marrow, adipose tissue and other tissues. In particular MSCs derived from Adipose tissue (ADSCs) are the most frequently used in regenerative medicine because they are easy to source, rapidly expandable in culture and excellent differentiation potential into adipocytes, chondrocytes and other cell types. Acetylcholine (ACh), is one of the most important neurotransmitter in central (CNS) and peripheral nervous system (PNS), playing important roles also in non-neural tissue, but its functions in MSCs are still not investigated...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29218427/the-role-of-nonautologous-and-autologous-adipose-derived-mesenchymal-stem-cell-in-acute-pyelonephritis
#2
Shabnam Sabetkish, Nastaran Sabetkish, Mahba Ataei Talebi, Shahnaz Halimi, Abdol-Mohammad Kajbafzadeh
We compared the therapeutic effects of autologous and nonautologous adipose-derived mesenchymal stem cell (ADMSC), in ameliorating the renal function in a rabbit model of acute pyelonephritis. The difference of perirenal and neck subcutaneous ADMSCs were also evaluated. Twenty female rabbits were apportioned to 5 groups. In group I (n = 4), the rabbits were injected direct inoculation of Escherichia coli (E. coli) into the right kidney. In group II (n = 4), autologous ADMSCs obtained from nape adipose tissue were injected into the subcapsular space 1 week after E...
December 7, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29218085/identification-and-differential-expression-of-micrornas-in-1-25-dihydroxyvitamin-d3-induced-osteogenic-differentiation-of-human-adipose-derived-mesenchymal-stem-cells
#3
Huijie Gu, Jun Xu, Zhongyue Huang, Liang Wu, Kaifeng Zhou, Yiming Zhang, Jiong Chen, Jiangni Xia, Xiaofan Yin
The aim of this study was to identify specific microRNAs (miRNAs) and their regulatory roles in the process of 1, 25-dihydroxyvitamin D3-induced (VD3-induced) osteogenic differentiation of human adipose-derived Mesenchymal stem cells (hAMSCs). The differentially expressed miRNAs in VD3-induced hAMSCs was examined. The putative target genes of these miRNAs were predicted. A total of 76 conserved miRNAs, including 18 miRNAs were significantly up-regulated and 58 miRNAs were significantly downregulated, and significantly differentially expressed between the two samples...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29214990/rna-sequencing-reveals-a-transcriptomic-portrait-of-human-mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-palatine-tonsils
#4
Kyung-Ah Cho, Minhwa Park, Yu-Hee Kim, So-Youn Woo, Kyung-Ha Ryu
Human mesenchymal stem cells (MSCs) are adult multipotent cells that have plasticity and inhabit the stroma of diverse tissues. The potential utility of MSCs has been heavily investigated in the fields of regenerative medicine and cell therapy. However, MSCs represent diverse populations that may depend on the tissue of origin. Thus, the ability to identify specific MSC populations has remained difficult. Using RNA sequencing, we analyzed the whole transcriptomes of bone marrow-derived MSCs (BMs), adipose tissue-derived MSCs (AMs), and tonsil-derived MSCs (TMs)...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214313/rna-sequencing-gene-expression-profiling-of-orbital-adipose-derived-stem-cell-population-implicate-hox-genes-and-wnt-signaling-dysregulation-in-the-pathogenesis-of-thyroid-associated-orbitopathy
#5
Wensi Tao, Juan A Ayala-Haedo, Matthew G Field, Daniel Pelaez, Sara T Wester
Purpose: The purpose of this study was to characterize the intrinsic cellular properties of orbital adipose-derived stem cells (OASC) from patients with thyroid-associated orbitopathy (TAO) and healthy controls. Methods: Orbital adipose tissue was collected from a total of nine patients: four controls and five patients with TAO. Isolated OASC were characterized with mesenchymal stem cell-specific markers. Orbital adipose-derived stem cells were differentiated into three lineages: chondrocytes, osteocytes, and adipocytes...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29212384/articular-and-subcutaneous-adipose-tissues-of-rheumatoid-arthritis-patients-represent-equal-sources-of-immunoregulatory-mesenchymal-stem-cells
#6
Urszula Skalska, Ewa Kuca-Warnawin, Anna Kornatka, Iwona Janicka, Urszula Musiałowicz, Tomasz Burakowski, Ewa Kontny
Adipose-derived mesenchymal stem cells (ASCs) have immunoregulatory properties, but their activity is dependent on signals provided by the local microenvironment. It is likely that highly inflammatory milieu of rheumatoid joint affects ASCs activity. To test this hypothesis, the function of rheumatoid ASCs derived from articular adipose tissue (AT-ASCs) and ASCs derived from subcutaneous adipose tissue (Sc-ASCs) has been analysed. Articular adipose tissue (infrapatellar fat pad) and subcutaneous adipose tissue (from the site of skin closure with sutures) were obtained from rheumatoid arthritis (RA) patients undergoing total knee joint replacement surgery...
December 6, 2017: Autoimmunity
https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#7
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29209609/culture-medium-supplements-derived-from-human-platelet-and-plasma-cell-commitment-and-proliferation-support
#8
Anita Muraglia, Van Thi Nguyen, Marta Nardini, Massimo Mogni, Domenico Coviello, Beatrice Dozin, Paolo Strada, Ilaria Baldelli, Matteo Formica, Ranieri Cancedda, Maddalena Mastrogiacomo
Present cell culture medium supplements, in most cases based on animal sera, are not fully satisfactory especially for the in vitro expansion of cells intended for human cell therapy. This paper refers to (i) an heparin-free human platelet lysate (PL) devoid of serum or plasma components (v-PL) and (ii) an heparin-free human serum derived from plasma devoid of PL components (Pl-s) and to their use as single components or in combination in primary or cell line cultures. Human mesenchymal stem cells (MSC) primary cultures were obtained from adipose tissue, bone marrow, and umbilical cord...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29209319/mesenchymal-stem-cells-induce-expression-of-cd73-in-human-monocytes-in-vitro-and-in-a-swine-model-of-myocardial-infarction-in-vivo
#9
Marta Monguió-Tortajada, Santiago Roura, Carolina Gálvez-Montón, Marcella Franquesa, Antoni Bayes-Genis, Francesc E Borràs
The ectoenzymes CD39 and CD73 regulate the purinergic signaling through the hydrolysis of adenosine triphosphate (ATP)/ADP to AMP and to adenosine (Ado), respectively. This shifts the pro-inflammatory milieu induced by extracellular ATP to the anti-inflammatory regulation by Ado. Mesenchymal stem cells (MSCs) have potent immunomodulatory capabilities, including monocyte modulation toward an anti-inflammatory phenotype aiding tissue repair. In vitro, we observed that human cardiac adipose tissue-derived MSCs (cATMSCs) and umbilical cord MSCs similarly polarize monocytes toward a regulatory M2 phenotype, which maintained the expression of CD39 and induced expression of CD73 in a cell contact dependent fashion, correlating with increased functional activity...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29208029/tissue-source-determines-the-differentiation-potentials-of-mesenchymal-stem-cells-a-comparative-study-of-human-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue
#10
Liangliang Xu, Yamei Liu, Yuxin Sun, Bin Wang, Yunpu Xiong, Weiping Lin, Qiushi Wei, Haibin Wang, Wei He, Bin Wang, Gang Li
BACKGROUND: Mesenchymal stem cells (MSCs) possess intrinsic regeneration capacity as part of the repair process in response to injury, such as fracture or other tissue injury. Bone marrow and adipose tissue are the major sources of MSCs. However, which cell type is more effective and suitable for cell therapy remains to be answered. The intrinsic molecular mechanism supporting the assertion has also been lacking. METHODS: Human bone marrow-derived MSCs (BMSCs) and adipose tissue-derived MSCs (ATSCs) were isolated from bone marrow and adipose tissue obtained after total hip arthroplasty...
December 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29207475/function-of-micrornas-in-the-osteogenic-differentiation-and-therapeutic-application-of-adipose-derived-stem-cells-ascs
#11
REVIEW
Walter M Hodges, Frederick O'Brien, Sadanand Fulzele, Mark W Hamrick
Traumatic wounds with segmental bone defects represent substantial reconstructive challenges. Autologous bone grafting is considered the gold standard for surgical treatment in many cases, but donor site morbidity and associated post-operative complications remain a concern. Advances in regenerative techniques utilizing mesenchymal stem cell populations from bone and adipose tissue have opened the door to improving bone repair in the limbs, spine, and craniofacial skeleton. The widespread availability, ease of extraction, and lack of immunogenicity have made adipose-derived stem cells (ASCs) particularly attractive as a stem cell source for regenerative strategies...
December 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29207162/mir-33a-hinders-the-differentiation-of-adipose-mesenchymal-stem-cells-towards-urothelial-cells-in-an-inductive-condition-by-targeting-%C3%AE-%C3%A2-catenin-and-tgfr
#12
Gang Fan, Zhenzhou Xu, Xiang Hu, Mingfeng Li, Jie Zhou, Yong Zeng, Yu Xie
Tissue engineering technology offers an appealing approach for tissue reconstruction of the urothelium. Adipose‑derived mesenchymal stem cells (ADSCs) represent an abundant source for tissue engineering applications. However, ASCs primarily possess mesoderm lineage differentiation potential. It is difficult to induce differentiation of ASCs towards urothelial cells that are derived from the endoderm, although a recent findings have reported that a conditioned medium may drive ADSCs towards differentiation into the urothelium phenotype...
November 27, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29207059/therapeutic-effect-of-adipose%C3%A2-derived-stem-cell-transplantation-on-optic-nerve-injury-in-rats
#13
Xu Li, Shanyao Zhao, Liangjun Wang
Adipose-derived stem cells (ADSCs) are mesenchymal stem cells in adipose tissue. ADSCs may differentiate into cells and tissues of different embryonic germ layers under specific inducing conditions. Previous studies have demonstrated that Schwann cells transformed from ADSCs can express nerve growth factors, generate nerve fibers and promote axonal regeneration in models of peripheral nerve injury. Stem cell transplantation is one of the potential techniques for the treatment of retinal ganglion cell injury...
November 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29205184/adipose-derived-mesenchymal-stem-cells-suppress-of-acute-rejection-in-small-bowel-transplantation
#14
Yu Zhang, Qinghong Meng, Yanyan Zhang, Xiaobo Chen, Yuliang Wang
BACKGROUND/AIMS: Adipose-derived mesenchymal stem cells (ADSCs) possess immunosuppressive activity and hold promise in autologous cell-based therapies. The aim of this study was to determine whether autologous ADSCs can improve outcomes in the rat small bowel transplantation (SBT) model. MATERIALS AND METHODS: Allogeneic SBT followed by implantation of autologous ADSCs through the penile vein was conducted in Brown-Norway (BN) donor rats with Lewis (LEW) recipient rats infused with phosphate buffered solution as the control...
November 2017: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/29196933/osmolyte-type-and-the-osmolarity-level-affect-chondrogenesis-of-mesenchymal-stem-cells
#15
Sorour Ahmadyan, Mahboubeh Kabiri, Hana Hanaee-Ahvaz, Ali Farazmand
The inductive effects of increased osmolarity on chondrogenesis are well approved. However, the effects of the osmolyte agent invoked to induce hyperosmolarity are largely neglected. Herein, we scrutinized how hyperosmotic conditions acquired by addition of different osmolytes would impact chondrogenesis. We briefly assessed whether such conditions would differentially affect hypertrophy and angiogenesis during MSC chondrogenesis. Chondrogenic and hypertrophic marker expression along with VEGF secretion during adipose-derived (AD)-MSC chondrogenesis under three osmolarity levels (350, 450, and 550 mOsm) using three different osmolytes (NaCl, sorbitol, and PEG) were assessed...
November 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29192407/de-novo-lipogenesis-and-desaturation-of-fatty-acids-during-adipogenesis-in-bovine-adipose-derived-mesenchymal-stem-cells
#16
Yongli Yue, Lichun Zhang, Xiang Zhang, Xueling Li, Haiquan Yu
Adipose-derived mesenchymal stem cells (ADSCs) are useful cell model to study adipogenesis and energy metabolism. However, the biological characteristics of bovine ADSCs (bADSCs) remain unclear. This study aimed to isolate and identify bADSCs and further investigate fatty acid (FA)-related gene expression and composition of FAs during adipogenesis. The growth curve showed the bADSCs of P5 cells had rapid proliferation superior to P10-P50. The colony formation assay showed colony number of P5 cells was higher than that of P50 cells (51...
November 30, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29192282/functionalized-gold-nanorod-nanocomposite-system-to-modulate-differentiation-of-human-mesenchymal-stem-cells-into-neural-like-progenitors
#17
Karrer M Alghazali, Steven D Newby, Zeid A Nima, Rabab N Hamzah, Fumiya Watanabe, Shawn E Bourdo, Thomas J Masi, Stacy M Stephenson, David E Anderson, Madhu S Dhar, Alexandru S Biris
A 2D multifunctional nanocomposite system of gold nanorods (AuNRs) was developed. Gold nanorods were functionalized via polyethylene glycol with a terminal amine, and, were characterized using transmission and scanning electron microscopy, ultra violet-visible and X-ray photoelectron spectroscopy, and Zeta-potential. The system was cytocompatible to and maintained the integrity of Schwann cells. The neurogenic potential of adipose tissue - derived human mesenchymal stem cells (hMSCs) was evaluated in vitro...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29189996/zinc-sulphate-mediates-the-stimulation-of-cell-proliferation-of-rat-adipose-tissue-derived-mesenchymal-stem-cells-under-high-intensity-of-emf-exposure
#18
Ezzatollah Fathi, Raheleh Farahzadi
Unlike the role of mesenchymal stem cells (MSCs) in regenerative medicine, their application in cell therapy can be complicated by factors such as a reduction in the proliferation potential, senescent tendency of MSCs to expand, and an age-dependent decline in their number and functions. It was shown that there is an association between exposure to electromagnetic fields (EMFs) and response to stress, cell proliferation, aging, and cell death. Furthermore, the zinc ion, as an essential trace element, was reported to be involved in the regulation of the growth and cell proliferation...
November 30, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/29187052/mesenchymal-stem-cell-therapy-prevents-abortion-in-cba-j-%C3%A3-dba-2-mating
#19
Bizhan Sadighi-Moghaddam, Amir Salek Farrokhi, Hassan Namdar Ahmadabad, Mehdi Barati, Seyed Mohammad Moazzeni
Immunological disorders are among the main causes of recurrent spontaneous abortions (RSAs). Mesenchymal stem cells (MSCs) have been shown to modulate various aspects of immune responses. It seems that MSCs may improve the immunological conditions in immune-mediated RSA. The aim of this study is the reduction of resorption in RSA mouse model through MSCs therapy. The adipose-derived MSCs were administered intraperitoneal to pregnant CBA/J mice on day 4.5 of gestation in abortion-prone matting. On day 13.5 of pregnancy, abortion rates were calculated and transforming growth factor-β (TGF-β), interleukin 10 (IL-10), interferon γ (IFN-γ), and tumor necrosis factor α (TNF-α) gene expression in deciduas were evaluated by real-time polymerase chain reaction (PCR)...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29186789/impact-of-antibiotics-on-the-proliferation-and-differentiation-of-human-adipose-derived-mesenchymal-stem-cells
#20
(no author information available yet)
Adipose tissue is a promising source of mesenchymal stem cells. Their potential to differentiate and regenerate other types of tissues may be affected by several factors. This may be due to in vitro cell-culture conditions, especially the supplementation with antibiotics. The aim of our study was to evaluate the effects of a penicillin-streptomycin mixture (PS), amphotericin B (AmB), a complex of AmB with copper (II) ions (AmB-Cu2+) and various combinations of these antibiotics on the proliferation and differentiation of adipose-derived stem cells in vitro...
November 24, 2017: International Journal of Molecular Sciences
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