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https://www.readbyqxmd.com/read/28070591/-the-importance-of-conservation-of-rare-domestic-animal-breeds-using-the-example-of-arc-warder
#1
Kai Frölich, Anabell Jandowsky
The domestic animals descend from various wild ancestors. Thus, for example, of the wolf, the dog (15  000 BC), of the Bezoar goat the goat (10  000 BC), of the Asian mouflon the sheep (10  000 BC), of the wild boar the pig (8000 BC) and of the aurochs the cattle (7000 BC). Domestication has dramatically changed our culture and led to a great diversity of animal breeds. This is a unique cultural and historical treasure, which we have to preserve for future generations. The zoological park Arc Warder is Europe's largest center for rare and endangered domestic animal breeds...
January 10, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/28057738/a-population-of-hematopoietic-stem-cells-derives-from-gata4-expressing-progenitors-located-in-the-placenta-and-lateral-mesoderm-of-mice
#2
Ana Cañete, Rita Carmona, Laura Ariza, Maria Jose Sanchez, Anabel Rojas, Ramon Muñoz-Chápuli
GATA transcription factors are expressed in mesoderm and endoderm during development. GATA1-3, but not GATA4, are critically involved in hematopoiesis. An enhancer (G2) of the mouse Gata4 gene directs its expression throughout the lateral mesoderm and the allantois, beginning at E7.5, becoming restricted to the septum transversum by E10.5, and disappearing by midgestation. We have studied the developmental fate of the G2-Gata4 cell lineage using a G2-Gata4Cre;R26REYFP mouse line. We found a substantial number of YFP+ hematopoietic cells of lymphoid, myeloid and erythroid lineages in embryos...
January 5, 2017: Haematologica
https://www.readbyqxmd.com/read/28042884/pharmacokinetics-of-the-antimicrobial-drug-sulfanilamide-is-altered-in-a-preclinical-model-of-vascular-calcification
#3
Anabel Brandoni, Adriana Mónica Torres
In vascular smooth muscle, calcium overload is linked to advancing age. The pharmacokinetics of sulfanilamide (SA), a compound with antibacterial properties, was evaluated in a preclinical model of vascular calcification. SA was used since it is useful to study possible modifications in the renal and hepatic management of drugs. Vascular calcification was induced by administration of a single high dose of vitamin D3 to rats (treated group) 10 days before the experiments. A parallel control group was processed...
January 2, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/27989941/a-multicentric-study-to-evaluate-the-use-of-relative-retention-times-in-targeted-proteomics
#4
Vital Vialas, Núria Colomé-Calls, Joaquín Abian, Kerman Aloria, Gloria Alvarez-Llamas, Oreto Antúnez, Jesus M Arizmendi, Mikel Azkargorta, Silvia Barceló-Batllori, María G Barderas, Francisco Blanco, J Ignacio Casal, Vanessa Casas, Carolina de la Torre, Eduardo Chicano-Gálvez, Felix Elortza, Guadalupe Espadas, Josep M Estanyol, Joaquín Fernandez-Irigoyen, Patricia Fernandez-Puente, María José Fidalgo, Manuel Fuentes, Marina Gay, Concha Gil, Alexandre Hainard, Maria Luisa Hernaez, Nieves Ibarrola, Arthur T Kopylov, Antonio Lario, Juan Antonio Lopez, María López-Lucendo, Miguel Marcilla, Anabel Marina-Ramírez, Gyorgy Marko-Varga, Luna Martín, Maria I Mora, Esperanza Morato-López, Javier Muñoz, Maria Antonia Odena, Eliandre de Oliveira, Irene Orera, Ignacio Ortea, Carla Pasquarello, Kevin B Ray, Melinda Rezeli, Isabel Ruppen, Eduard Sabidó, Manuel M Sanchez Del Pino, Jaime Sancho, Enrique Santamaría, Jesus Vazquez, Marta Vilaseca, Fernando Vivanco, James J Walters, Victor G Zgoda, Fernando J Corrales, Francesc Canals, Alberto Paradela
: Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT), has lately emerged as a powerful tool. The robustness, reproducibility and utility of this strategy were examined for the first time in a multicentric setting, involving 28 laboratories that included 24 of the Spanish network of proteomics laboratories (ProteoRed-ISCIII)...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27958606/from-the-molecule-to-the-mole-improving-heterogeneous-copper-catalyzed-click-chemistry-using-single-molecule-spectroscopy
#5
Bowen Wang, Javier Durantini, Matthew R Decan, Jun Nie, Anabel E Lanterna, Juan C Scaiano
Single molecule spectroscopy (SMS) inspired the optimization of a heterogeneous 'click' catalyst leading to enhanced yields of the Cu-catalyzed reaction of azides with terminal alkynes. Changes in SMS data after optimization confirm the improvements in catalyst performance.
December 22, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27921162/multicentre-randomized-trial-of-the-ajust%C3%A2-single-incision-sling-compared-to-the-align%C3%A2-transobturator-tape-sling
#6
Jordi Sabadell, Marta Palau-Gené, Eva Huguet, Anabel Montero-Armengol, Sabina Salicrú, Jose L Poza
INTRODUCTION AND HYPOTHESIS: Tension-free suburethral tapes have become the first-line surgical treatment for female stress urinary incontinence. Single-incision midurethral slings (SIMS) were introduced with the aim of offering similar efficacy with reduced morbidity, particularly postoperative pain. The objective of this study was to compare the effectiveness and complications of the Ajust™ SIMS and the Align™ transobturator tape sling. METHODS: We performed a randomized controlled trial with a noninferiority design...
December 5, 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/27917052/the-blowflies-of-the-madeira-archipelago-species-diversity-distribution-and-identification-diptera-calliphoridaes-l
#7
Catarina Prado E Castro, Krzysztof Szpila, Anabel Martínez-Sánchez, Rego, Isamberto Silva, Artur R M Serrano, Mário Boieiro
Knowledge on the taxonomic diversity and distribution of blowflies from the Madeira Archipelago is updated. New and interesting findings are reported for poorly studied islands and islets of this archipelago, together with a brief analysis of the diversity of Macaronesian Calliphoridae s. l. Seven blowfly species were collected during this study, including the first records of Calliphora vicina Robineau-Desvoidy, 1830, Chrysomya albiceps (Wiedemann, 1819), Lucilia sericata (Meigen, 1826), Pollenia rudis (Fabricius, 1794) and Stomorhina lunata (Fabricius, 1805) from Porto Santo, and of Calliphora vicina, Lucilia sericata and Stomorhina lunata from Desertas Islands...
2016: ZooKeys
https://www.readbyqxmd.com/read/27916458/a-sensor-for-low-environmental-oxygen-in-the-mouse-main-olfactory-epithelium
#8
Katherin Bleymehl, Anabel Pérez-Gómez, Masayo Omura, Ana Moreno-Pérez, David Macías, Zhaodai Bai, Randall S Johnson, Trese Leinders-Zufall, Frank Zufall, Peter Mombaerts
Sensing the level of oxygen in the external and internal environments is essential for survival. Organisms have evolved multiple mechanisms to sense oxygen. No function in oxygen sensing has been attributed to any mammalian olfactory system. Here, we demonstrate that low environmental oxygen directly activates a subpopulation of sensory neurons in the mouse main olfactory epithelium. These neurons express the soluble guanylate cyclase Gucy1b2 and the cation channel Trpc2. Low oxygen induces calcium influx in these neurons, and Gucy1b2 and Trpc2 are required for these responses...
December 21, 2016: Neuron
https://www.readbyqxmd.com/read/27914927/novel-rna-viruses-producing-simultaneous-covert-infections-in-ceratitis-capitata-correlations-between-viral-titers-and-host-fitness-and-implications-for-sit-programs
#9
Angel Llopis-Giménez, Rosa Maria González, Anabel Millán-Leiva, Marta Catalá, Elena Llacer, Alberto Urbaneja, Salvador Herrero
The Mediterranean fruit fly (medfly), Ceratitis capitata is a highly polyphagous pest, which infests multiple species of fruits and vegetables worldwide. In addition to the traditional control with chemical insecticides, sterile insect technique (SIT) has been implemented in integrated programs worldwide, and has become an essential measure for the control of this pest. A key issue for SIT is to release sterile males that are sufficiently competitive with males from the wild population. Using sequence information available in public databases, three novel picornaviruses infecting medflies were discovered and named as C...
December 1, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27913693/health-equity-diversity-and-inclusion
#10
Anabell Castro Thompson
No abstract text is available yet for this article.
December 2016: Hispanic Health Care International: the Official Journal of the National Association of Hispanic Nurses
https://www.readbyqxmd.com/read/27854562/full-shift-and-task-specific-upper-extremity-muscle-activity-among-us-large-herd-dairy-parlour-workers
#11
David I Douphrate, Nathan B Fethke, Matthew W Nonnenmann, Anabel Rodriguez, Robert Hagevoort, David Gimeno Ruiz de Porras
US large-herd dairy parlour workers experience a high prevalence of musculoskeletal symptoms in the upper extremity. The purpose of this study was to estimate and compare full-shift and task-specific muscle activity of the upper extremity among parlour workers. Surface electromyography data were recorded continuously throughout a full work shift for each participant (n = 60). For a subset of participants (n = 33), muscular effort was estimated for milking task cycles. Lower muscle activity levels and higher per cent muscular rest was observed among rotary parlour participants as compared to herringbone and parallel parlour participants for anterior deltoid, forearm flexor and forearm extensor muscles...
December 23, 2016: Ergonomics
https://www.readbyqxmd.com/read/27850412/774-withdrawal-syndrome-incidence-and-risk-factors-in-a-pediatric-intensive-care-unit
#12
Kristie Rodriguez-Otero, Anabel Puig-Ramos, Carlos Lopez-Ortiz, Samuel Pabon-Rivera, Gabriel De Jesus-Astacio, Ricardo Nieves, Maria Villar-Prados, Ricardo Garcia-De Jesus
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27842609/a-recombinant-multi-antigen-vaccine-formulation-containing-babesia-bovis-merozoite-surface-antigens-msa-2a1-msa-2b-and-msa-2c-elicits-invasion-inhibitory-antibodies-and-ifn-%C3%AE-producing-cells
#13
Alba Marina Gimenez, Katia S Françoso, Jonatan Ersching, Marcelo Y Icimoto, Vitor Oliveira, Anabel E Rodriguez, Leonhard Schnittger, Monica Florin-Christensen, Mauricio M Rodrigues, Irene S Soares
BACKGROUND: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B...
November 14, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27772801/wilms-tumour-gene-1-wt1-mutations-in-acute-myeloid-leukemia-aml
#14
Joëlle Marivel, Anabel Kearney, Anna Nelson, Silvia Ling
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27736991/loss-of-pancreas-upon-activated-wnt-signaling-is-concomitant-with-emergence-of-gastrointestinal-identity
#15
Jose Luis Muñoz-Bravo, Alvaro Flores-Martínez, Griselda Herrero-Martin, Sapna Puri, Makoto Mark Taketo, Anabel Rojas, Matthias Hebrok, David A Cano
Organ formation is achieved through the complex interplay between signaling pathways and transcriptional cascades. The canonical Wnt signaling pathway plays multiple roles during embryonic development including patterning, proliferation and differentiation in distinct tissues. Previous studies have established the importance of this pathway at multiple stages of pancreas formation as well as in postnatal organ function and homeostasis. In mice, gain-of-function experiments have demonstrated that activation of the canonical Wnt pathway results in pancreatic hypoplasia, a phenomenon whose underlying mechanisms remains to be elucidated...
2016: PloS One
https://www.readbyqxmd.com/read/27714692/astrocyte-dysfunction-in-developmental-neurometabolic-diseases
#16
Silvia Olivera-Bravo, Eugenia Isasi, Anabel Fernández, Gabriela Casanova, Juan Carlos Rosillo, Luigi Barbeito
Astrocytes play crucial roles in maintaining brain homeostasis and in orchestrating neural development, all through tightly coordinated steps that cooperate to maintain the balance needed for normal development. Here, we review the alterations in astrocyte functions that contribute to a variety of developmental neurometabolic disorders and provide additional data on the predominant role of astrocyte dysfunction in the neurometabolic neurodegenerative disease glutaric acidemia type I. Finally, we describe some of the therapeutical approaches directed to neurometabolic diseases and discuss if astrocytes can be possible therapeutic targets for treating these disorders...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27693237/transcranial-direct-current-stimulation-in-mesial-temporal-lobe-epilepsy-and-hippocampal-sclerosis
#17
Daniel San-Juan, Dulce Anabel Espinoza López, Rafael Vázquez Gregorio, Carlos Trenado, Maricarmen Fernández-González Aragón, León Morales-Quezada, Axel Hernandez Ruiz, Flavio Hernandez-González, Alejandro Alcaraz-Guzmán, David J Anschel, Felipe Fregni
BACKGROUND: Transcranial direct current stimulation (tDCS) has been evaluated in medication refractory epilepsy patients. The results have been inconclusive and protocols have varied between studies. OBJECTIVE: To evaluate the safety and efficacy of two protocols of tDCS in adult patients with mesial temporal lobe epilepsy and hippocampal sclerosis (MTLE-HS). METHODS: This is a randomized placebo-controlled, double-blinded clinical trial, with 3 arms, 3 sessions, 5 sessions and placebo stimulation...
August 31, 2016: Brain Stimulation
https://www.readbyqxmd.com/read/27683825/coa7-c1orf163-resa1-mutations-associated-with-mitochondrial-leukoencephalopathy-and-cytochrome-c-oxidase-deficiency
#18
Anabel Martinez Lyons, Anna Ardissone, Aurelio Reyes, Alan J Robinson, Isabella Moroni, Daniele Ghezzi, Erika Fernandez-Vizarra, Massimo Zeviani
BACKGROUND: Assembly of cytochrome c oxidase (COX, complex IV, cIV), the terminal component of the mitochondrial respiratory chain, is assisted by several factors, most of which are conserved from yeast to humans. However, some of them, including COA7, are found in humans but not in yeast. COA7 is a 231aa-long mitochondrial protein present in animals, containing five Sel1-like tetratricopeptide repeat sequences, which are likely to interact with partner proteins. METHODS: Whole exome sequencing was carried out on a 19 year old woman, affected by early onset, progressive severe ataxia and peripheral neuropathy, mild cognitive impairment and a cavitating leukodystrophy of the brain with spinal cord hypotrophy...
September 28, 2016: Journal of Medical Genetics
https://www.readbyqxmd.com/read/27673341/heterogeneous-photocatalytic-click-chemistry
#19
Bowen Wang, Javier Durantini, Jun Nie, Anabel E Lanterna, Juan C Scaiano
Copper-doped semiconductors are designed to photoassist the alkyne-azide cycloaddition catalysis by Cu(I). Upon irradiation, injection of electrons from the semiconductor into copper oxide nanostructures produces the catalytic Cu(I) species. The new catalysts are air- and moisture-tolerant and can be readily recovered after use and reused several times.
October 12, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27642710/conditional-deletion-of-wt1-in-the-septum-transversum-mesenchyme-causes-congenital-diaphragmatic-hernia-in-mice
#20
Rita Carmona, Ana Cañete, Elena Cano, Laura Ariza, Anabel Rojas, Ramon Muñoz-Chápuli
Congenital diaphragmatic hernia (CDH) is a severe birth defect. Wt1-null mouse embryos develop CDH but the mechanisms regulated by WT1 are unknown. We have generated a murine model with conditional deletion of WT1 in the lateral plate mesoderm, using the G2 enhancer of the Gata4 gene as a driver. 80% of G2-Gata4(Cre);Wt1(fl/fl) embryos developed typical Bochdalek-type CDH. We show that the posthepatic mesenchymal plate coelomic epithelium gives rise to a mesenchyme that populates the pleuroperitoneal folds isolating the pleural cavities before the migration of the somitic myoblasts...
2016: ELife
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