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Facilitated positional release

Vincent Ronfard, Alain Vertes, Michael May, Anne Dupraz, Mark van Dyke, Yves Bayon
"Evaluating the Past & Present of Regenerative Medicine (RM)" was the first part of an Industry Symposium dedicated to the subject during the 2015 World TERMIS Congress in Boston. This working session presented a critical review of the current RM landscape in Europe and North America with possible projections for the future. Interestingly, the RM development cycle seems to obey the Gartner hype cycle, now at the enlightenment phase, after past exaggerated expectations and discouragements, as suggested by increasing numbers of clinical trials and recent market approvals of RM solutions in both Europe (Glybera & Holoclar® from Chiesi Pharma and Strimvelis® from GSK) and Japan (Remestemcel-L from Mesoblast® )...
October 20, 2016: Tissue Engineering. Part B, Reviews
Koji Nakamura, Kenjiro Sawada, Yasuto Kinose, Akihiko Yoshimura, Aska Toda, Erika Nakatsuka, Kae Hashimoto, Seiji Mabuchi, Ken-Ichirou Morishige, Hirohisa Kurachi, Ernst Lengyel, Tadashi Kimura
: Epithelial ovarian cancer (EOC) cells metastasize within the peritoneal cavity and directly encounter human peritoneal mesothelial cells (HPMCs) as the initial step of metastasis. The contact between ovarian cancer cells and the single layer of mesothelial cells involves direct communications that modulate cancer progression but the mechanisms are unclear. One candidate mediating cell-cell communications are exosomes, 30-100 nm membrane vesicles of endocytic origin, through the cell-cell transfer of proteins, mRNAs, or microRNAs...
October 6, 2016: Molecular Cancer Research: MCR
Richard A Hawkins, Juan R Viña
A facilitative transport system exists on the blood-brain barrier (BBB) that has been tacitly assumed to be a path for glutamate entry to the brain. However, glutamate is a non-essential amino acid whose brain content is much greater than plasma, and studies in vivo show that glutamate does not enter the brain in appreciable quantities except in those small regions with fenestrated capillaries (circumventricular organs). The situation became understandable when luminal (blood facing) and abluminal (brain facing) membranes were isolated and studied separately...
October 8, 2016: Biology
Shweta Sharma, Ashwni Kumar Verma, Jyotsana Singh, B Venkatesh Teja, Naresh Mittapelly, Gitu Pandey, Sandeep Urandur, Ravi Shukla, Rituraj Konwar, Prabhat Ranjan Mishra
This study reports the development of Vitamin B6 (VitB6) modified pH sensitive charge reversal nanoparticles for efficient intracellular delivery of Doxorubicin (DOX). Herein, VitB6 was conjugated to stearic acid and the nanoparticles of the lipid were formulated by solvent injection method (DOX-B6-SA-NP). Due to the pKa (5.6) of VitB6, DOX-B6-SA-NP showed positive charge and enhanced release of DOX at pH 5. Confocal microscopy illustrated that DOX-B6-SA-NP treatment keep higher DOX accumulation inside the cells that conventional pH insensitive lipid nanoparticles (DOX-SA-NP)...
October 14, 2016: ACS Applied Materials & Interfaces
I Gupta, A Goyal, N K Singh, H N Yadav, P L Sharma
BACKGROUND: Attenuated cardioprotective effect of ischemic preconditioning (IPC) by reduced nitric oxide (NO) is a hallmark during diabetes mellitus (DM). Recently, we reported that the formation of caveolin-endothelial nitric oxide synthase (eNOS) complex decreases the release of NO, which is responsible for attenuation of IPC-induced cardioprotection in DM rat heart. Heme oxygenase-1 (HO-1) facilitates release of NO by disrupting caveolin-eNOS complex. The activity of HO-1 is decreased during DM...
October 12, 2016: Human & Experimental Toxicology
Marzia Del Re, Elisa Biasco, Stefania Crucitta, Lisa Derosa, Eleonora Rofi, Cinzia Orlandini, Mario Miccoli, Luca Galli, Alfredo Falcone, Guido W Jenster, Ron H van Schaik, Romano Danesi
BACKGROUND: The androgen receptor splice variant 7 (AR-V7) is associated with resistance to hormonal therapy in castration-resistant prostate cancer (CRPC). Due to limitations of the methods available for AR-V7 analysis, the identification of a reliable detection method may facilitate the use of this biomarker in clinical practice. OBJECTIVE: To confirm AR-V7 as a predictor of resistance to hormonal therapy and develop a new approach to assess AR-V7 by highly sensitive digital droplet polymerase chain reaction (ddPCR) in plasma-derived exosomal RNA...
August 26, 2016: European Urology
Alexandra M Moore, Lisa N Anderson, David C Chen
INTRODUCTION: Minimally invasive laparoscopic and robotic techniques for ventral hernia repair have evolved to achieve the benefits and minimize the limitations of both the open Rives-Stoppa sublay mesh repair and laparoscopic intraperitoneal onlay mesh (IPOM) repair. By combining the principles of a retromuscular repair with the benefits of a minimally invasive approach, these techniques attempt to decrease recurrence, increase functionality, exclude mesh from the viscera, limit infection and wound complications, and minimize pain...
October 6, 2016: Surgical Technology International
Yang Liu, Boran Wu, Yongxia Hao, Wei Zhu, Zhonggen Li, Xiaoli Chai
Mercury emission fluxes (MEFs) under different surface coverage conditions in a landfill were investigated in this study. The results show similar diel patterns of Hg emission flux under different coverage conditions, with peak fluxes occurring at midday and decreasing during night. We examined the effects of environmental factors on MEFs, such as the physiological characteristics of vegetation and meteorological conditions. The results suggest that growth of vegetation in the daytime facilitates the release of Hg in the anaerobic unit, while in the semi-aerobic unit, where vegetation had been removed, the higher mercury content of the cover soil prompted the photo-reduction pathway to become the main path of mercury release and increased MEFs...
October 6, 2016: Waste Management
Fabian Elgner, Huimei Ren, Regina Medvedev, Daniela Ploen, Kiyoshi Himmelsbach, Klaus Boller, Eberhard Hildt
BACKGROUND: HCV particles are described as lipoviroparticles which are released similar to VLDLs. However, the release mechanism is still poorly understood; the canonical ERGIC pathway as well as the endosome-dependent release has been proposed. Recently, also the role of exosomes in the transmission of HCV has been reported. Only a minor fraction of the de novo synthesized lipoviroparticles are finally released by the infected cell. METHODS: To investigate the relevance of MVBs for viral morphogenesis and release, the MVB inhibitor U18666A was used...
October 5, 2016: Journal of Virology
Sabine Ladrech, Jing Wang, Marc Mathieu, Jean-Luc Puel, Marc Lenoir
High mobility group box 1 (HMGB1) is a DNA-binding protein that facilitates gene transcription and may act extracellularly as a late mediator of inflammation. The roles of HMGB1 in the pathogenesis of the spiral ganglion neurons (SGNs) of the cochlea are currently unknown. In the present study, we tested the hypothesis that early phenotypical changes in the SGNs of the amikacin-poisoned rat cochlea are mediated by HMGB1. Our results showed that a marked downregulation of HMGB1 had occurred by completion of amikacin treatment, coinciding with acute damage at the dendrite extremities of the SGNs...
October 4, 2016: Histochemistry and Cell Biology
Simon J Hazenberg, Rob van Lier
In three experiments, we investigated the influence of object-specific sounds on haptic scene recognition without vision. Blindfolded participants had to recognize, through touch, spatial scenes comprising six objects that were placed on a round platform. Critically, in half of the trials, object-specific sounds were played when objects were touched (bimodal condition), while sounds were turned off in the other half of the trials (unimodal condition). After first exploring the scene, two objects were swapped and the task was to report, which of the objects swapped positions...
July 2016: I-Perception
Joakim Delaux, Carmen Ortiz Mellet, Christine Canaff, Elodie Fourré, Cédric Gaillard, Abdellatif Barakat, José M García Fernández, Jean-Michel Tatibouët, François Jérôme
We have investigated the effect of non-thermal atmospheric plasma (NTAP) on the structure of microcrystalline cellulose. In particular, by means of different characterization methods, we demonstrate that NTAP promotes the partial cleavage of the β-1,4 glycosidic bond of cellulose leading to the release of short-chain cellodextrins that are reassembled in situ, preferentially at the C6 position, to form branched glucans with either a glucosyl or anhydroglucosyl terminal residue. The ramification of cellulosic chain induced by NTAP yields branched glucans that are soluble in DMSO or in water, thus opening a straightforward access to processable glucans from cellulose...
September 30, 2016: Chemistry: a European Journal
Young Ah Seo, Ruvin Kumara, Herbert A Wetli, Marianne Wessling-Resnick
Divalent Metal Transporter-1 (DMT1) mediates dietary iron uptake across the intestinal mucosa and facilitates peripheral delivery of iron released by transferrin in the endosome. Here we report that classical cannabinoids (Δ(9)-THC), non-classical cannabinoids (CP55,940), aminoalkylindoles (WIN55,212-2), and endocannabinoids (anandamide) reduce (55)Fe and (54)Mn uptake by HEK293T(DMT1) cells stably expressing the transporter. siRNA knockdown of cannabinoid receptor-2 (CB2) abrogated inhibition. CB2 is G protein-coupled receptor that negatively regulates signal transduction cascades involving serine/threonine kinases...
September 28, 2016: Biochemical Journal
Yue Huang, Hao Li, Lei Wang, Xiaoxia Mao, Genxi Li
In this work, we have successfully designed a smart and flexible signal amplification method based on a newly synthesized hybrid nanocomposite with switchable enzyme activity for specific and sensitive protein detection. The smart hybrid nanocomposite synthesized here is initially loaded with quenched fluorophore and a unique aptamer-inhibited DNA polymerase. It then undergoes target protein-triggered release of the fluorophore and activation of the DNA polymerase, which can thereby promote multiple catalytic reactions and recycled use of the target protein, resulting in the generation of highly amplified signals...
October 4, 2016: ACS Applied Materials & Interfaces
Yichen Lu, Mei Yan Lee, Shu Zhu, Talid Sinno, Scott L Diamond
During clotting under flow, platelets bind and activate on collagen and release autocrinic factors such as ADP and thromboxane, while tissue factor (TF) on the damaged wall leads to localized thrombin generation. Towards patient-specific simulation of thrombosis, a multiscale approach was developed to account for: platelet signalling [neural network (NN) trained by pairwise agonist scanning (PAS), PAS-NN], platelet positions (lattice kinetic Monte Carlo, LKMC), wall-generated thrombin and platelet-released ADP/thromboxane convection-diffusion (partial differential equation, PDE) and flow over a growing clot (lattice Boltzmann)...
September 25, 2016: Mathematical Medicine and Biology: a Journal of the IMA
Xi Zoë Zhong, Xue Sun, Qi Cao, Gaofeng Dong, Raphael Schiffmann, Xian-Ping Dong
Efficient lysosomal Ca(2+) release plays an essential role in lysosomal trafficking. We have recently shown that lysosomal big conductance Ca(2+)-activated potassium (BK) channel forms a physical and functional coupling with the lysosomal Ca(2+) release channel Transient Receptor Potential Mucolipin-1 (TRPML1). BK and TRPML1 forms a positive feedback loop to facilitate lysosomal Ca(2+) release and subsequent lysosome membrane trafficking. However, it is unclear whether the positive feedback mechanism is common for other lysosomal storage diseases (LSDs) and whether BK channel agonists rescue abnormal lysosomal storage in LSDs...
September 27, 2016: Scientific Reports
Mario Milazzo, Serena Danti, Francesco Inglese, Godfried Jansen van Vuuren, Vera Gramigna, Gabriella Bonsignori, Andrea De Vito, Luca Bruschini, Cesare Stefanini, Stefano Berrettini
This study shows the fabrication of innovative ossicular replacement prostheses (ORPs) based on banked cortical bone using computer numerically controlled ultraprecision micromilling, as a substantial improvement of "second generation" ORPs. Our aim is to combine optimal middle ear compatibility and surgical manageability in a single device, by releasing off-the-shelf homograft ORPs provided with the appealing features of synthetic ORPs, such as lightness, safety, measurement accuracy, surface decoration, and geometric plasticity...
September 23, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Jianwen Zhao, Fengfeng Zhao, Xiyong Wang, Xiaobo Fan, Guoqiu Wu
A novel multifunctional nano-drug delivery system based on reversal of peptide charge was successfully developed for anticancer drug delivery and imaging. Mesoporous silica nano-particles (MSN) ~50 nm in diameter were chosen as the drug reservoirs, and their surfaces were modified with HIV-1 transactivator peptide-fluorescein isothiocyanate (TAT-FITC) and YSA-BHQ1. The short TAT peptide labeled with FITC was used to facilitate intranuclear delivery, while the YSA peptide tagged with the BHQ1 quencher group was used to specifically bind to the tumor EphA2 membrane receptor...
September 20, 2016: Oncotarget
Barbara K Möller, Wei Xuan, Tom Beeckman
In dicot root systems, lateral roots are in general regularly spaced along the longitudinal axis of the primary root to facilitate water and nutrient uptake. Recently, recurrent programmed cell death in the root cap of the growing root has been implicated in lateral root spacing. The root cap contains an auxin source that modulates lateral root patterning. Periodic release of auxin by dying root cap cells seems to trigger lateral root specification at regular intervals. However, it is currently unclear through which molecular mechanisms auxin restricts lateral root specification to specific cells along the longitudinal and radial axes of the root, or how environmental signals impact this process...
September 17, 2016: Current Opinion in Plant Biology
Penghe Wang, Hui Zhang, Jie Zuo, Dehua Zhao, Xiangxu Zou, Zhengjie Zhu, Nasreen Jeelani, Xin Leng, Shuqing An
The plants effect in subsurface flow constructed wetlands (SSF-CWs) is controversial, especially at low temperatures. Consequently, several SSF-CWs planted with Iris pseudacorus (CWI) or Typha orientalis Presl. (CWT) and several unplanted ones (CWC) were set up and fed with secondary effluent of sewage treatment plant during the winter in Eastern China. The 16S rDNA Illumina Miseq sequencing analysis indicated the positive effects of I. pseudacorus on the bacterial community richness and diversity in the substrate...
2016: Scientific Reports
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