keyword
MENU ▼
Read by QxMD icon Read
search

Facilitated positional release

keyword
https://www.readbyqxmd.com/read/27921105/ultrasmall-silver-nanoclusters-highly-efficient-antibacterial-activity-and-their-mechanisms
#1
Jian-Cheng Jin, Xiao-Juan Wu, Juan Xu, Bei-Bei Wang, Feng-Lei Jiang, Yi Liu
Silver materials have been widely used as antimicrobial agents. Notably, silver nanoparticles have emerged as a new generation of nanoproducts for biomedical and environmental applications in recent years. However, ultrasmall silver nanoclusters (NCs) (∼2 nm) have rarely been used to kill bacteria and their antibacterial mechanisms have not yet been fully elucidated. Herein, we studied the antibacterial activities of bifunctional fluorescent DHLA-AgNCs against three types of bacteria. The results showed that DHLA-AgNCs exhibited excellent antibacterial activities against Gram-negative E...
December 6, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27920500/design-synthesis-and-evaluation-of-vegf-sirna-crs-as-a-novel-vector-for-gene-delivery
#2
Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Small interfering RNA (siRNA) delivery is a prospective method in gene therapy, but it has application limitations such as negative charge, water solubility and high molecular weight. In this study, a safe and efficient nano-vector, CRS, was designed and synthesized to facilitate siRNA delivery. Physical and chemical properties of VEGF-siRNA/CRS were characterized by methods including scanning electron microscopy (SEM), transmission electron microscopy, zeta potential (ζ) measurement, drug-releasing rate measurement, gel electrophoresis and confocal microscopy...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27919800/increasing-the-fidelity-of-noncanonical-amino-acid-incorporation-in-cell-free-protein-synthesis
#3
Qinglei Gan, Chenguang Fan
BACKGROUND: Cell-free protein synthesis provides a robust platform for co-translational incorporation of noncanonical amino acid (ncAA) into proteins to facilitate biological studies and biotechnological applications. Recently, eliminating the activity of release factor 1 has been shown to increase ncAA incorporation in response to amber codons. However, this approach could promote mis-incorporation of canonical amino acids by near cognate suppression. METHODS: We performed a facile protocol to remove near cognate tRNA isoacceptors of the amber codon from total tRNAs, and used the phosphoserine (Sep) incorporation system as validation...
December 2, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27909672/arthroscopic-360%C3%A2-capsular-release-for-adhesive-capsulitis-in-the-lateral-decubitus-position
#4
Gregory L Cvetanovich, Timothy Leroux, Jason T Hamamoto, John D Higgins, Anthony A Romeo, Nikhil N Verma
Idiopathic adhesive capsulitis of the shoulder is a relatively common condition that results in pain and loss of motion due to capsular thickening and fibrosis. Most cases are successfully treated with conservative management including physical therapy and intra-articular steroid injections. If conservative management fails, arthroscopic capsular release allows precise release of thickened capsular tissue with a lower risk of complications and less soft-tissue trauma than manipulation under anesthesia alone...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27906160/mechanistic-insights-into-the-alternative-translation-termination-by-arfa-and-rf2
#5
Chengying Ma, Daisuke Kurita, Ningning Li, Yan Chen, Hyouta Himeno, Ning Gao
During cellular translation of mRNAs by ribosomes, various situations that would result in the pausing or stalling of translation apparatus at elongation and termination steps often occur(1-6). Except for programmed stalling, which is usually utilized by the cells to exert regulatory purposes(5,7,8), ribosomes stalled on mRNAs need to be terminated and recycled to maintain adequate cellular translation capacity(9). A large source of ribosome stalling originates in aberrant mRNAs that lack a stop codon. Transcriptional errors and misprocessing of primary transcripts, as well as undesired mRNA cleavage, all contribute to the formation of non-stop mRNAs...
December 1, 2016: Nature
https://www.readbyqxmd.com/read/27888616/ccl2-egf-positive-feedback-loop-between-cancer-cells-and-macrophages-promotes-cell-migration-and-invasion-in-head-and-neck-squamous-cell-carcinoma
#6
Lu Gao, Feng-Qin Wang, Hui-Min Li, Jie-Gang Yang, Jian-Gang Ren, Ke-Fei He, Bing Liu, Wei Zhang, Yi-Fang Zhao
Head and neck squamous cell carcinoma (HNSCC) represents the most frequent malignancy in the head and neck region, and the survival rate has not been improved significantly over the past three decades. It has been reported the infiltrated macrophages contribute to the malignant progression of HNSCC. However, the crosstalk between macrophages and cancer cells remains poorly understood. In the present study, we explored interactions between monocytes/macrophages and HNSCC cells by establishing the direct co-culture system, and found that the crosstalk promoted the migration and invasion of cancer cells by enhancing the invadopodia formation through a CCL2/EGF positive feedback loop...
November 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27887910/assessment-of-serum-level-of-corticotropin-releasing-factor-in-primary-nocturnal-enuresis
#7
Ayat A Motawie, Amany M Abd Al-Aziz, Hanan M Hamed, Amany A A Fatouh, Mona A M Awad, Amany Abd El-Ghany
INTRODUCTION: Primary nocturnal enuresis is one of the sleep related phenomena characterized by disruption in the relationship between arousal and urination. Corticotropin-releasing factor (CRF) is a neurohormone released from the paraventricular nucleus of the hypothalamus into the median eminence to elicit release of adrenocorticotrophin from the anterior pituitary. It may act to modulate autonomic function and behavior in concert with the endocrine effects. Conflicting animal studies about the role of CRF in micturition, either facilitating or inhibiting, have been raised...
October 24, 2016: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/27885530/protein-secretion-in-gram-positive-bacteria-from-multiple-pathways-to-biotechnology
#8
Jozef Anné, Anastassios Economou, Kristel Bernaerts
A number of Gram-positive bacteria are important players in industry as producers of a diverse array of economically interesting metabolites and proteins. As discussed in this overview, several Gram-positive bacteria are valuable hosts for the production of heterologous proteins. In contrast to Gram-negative bacteria, proteins secreted by Gram-positive bacteria are released into the culture medium where conditions for correct folding are more appropriate, thus facilitating the isolation and purification of active proteins...
November 25, 2016: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/27883931/disassembly-of-amphiphilic-small-molecular-prodrug-with-fluorescence-switch-induced-by-ph-and-folic-acid-receptors-for-targeted-delivery-and-controlled-release
#9
Zhigang Xu, Xiaoxiao Shi, Meili Hou, Peng Xue, Yong-E Gao, Shiying Liu, Yuejun Kang
We develop a new type of pH-responsive amphiphilic small molecular prodrug by conjugating folic acid with anti-tumour doxorubicin via a hydrazone bond. This prodrug is featured by high and precise drug loading (55.4wt%), which can self-assemble into micellar nanoparticles in neutral environment while disassemble in the presence of tumour cells expressing folic acid receptors or the acidic tumoral endosomal environment. The prodrug nanoparticles can effectively improve anticancer efficacy due to the features of pH-triggered drug release and targeted delivery...
November 17, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27880899/lc3c-contributes-to-vpu-mediated-antagonism-of-bst2-tetherin-restriction-on-hiv-1-release-through-a-non-canonical-autophagy-pathway
#10
Ursula Madjo, Olivier Leymarie, Stéphane Frémont, Aurelia Kuster, Mélanie Nehlich, Sarah Gallois-Montbrun, Katy Janvier, Clarisse Berlioz-Torrent
BST2 (bone marrow stromal antigen 2)/tetherin is a restriction factor of enveloped viruses, which blocks the release of viral particles. HIV-1 encodes proteins that antagonize this innate barrier, including the accessory protein Vpu. Here, we investigate whether the autophagy pathway and/or ATG proteins are hijacked by HIV-1 Vpu to circumvent BST2 restriction of viral release. We report that BST2 and Vpu are present in LC3-positive compartments. We found that Vpu selectively interacts with the ATG8 ortholog LC3C through the Vpu L63VEM66 sequence...
November 22, 2016: Cell Reports
https://www.readbyqxmd.com/read/27859072/a-broadleaf-species-enhances-an-autotoxic-conifers-growth-through-belowground-chemical-interactions
#11
Zhi-Chao Xia, Chui-Hua Kong, Long-Chi Chen, Peng Wang, Si-Long Wang
Plants may affect the performance of neighboring plants either positively or negatively through interspecific and intraspecific interactions. Productivity of mixed-species systems is ultimately the net result of positive and negative interactions among the component species. Despite increasing knowledge of positive interactions occurring in mixed-species tree systems, relatively little is known about the mechanisms underlying such interactions. Based on data from 25-year-old experimental stands in situ and a series of controlled experiments, we test the hypothesis that a broadleaf, non-N fixing species, Michelia macclurei, facilitates the performance of an autotoxic conifer Chinese fir (Cunninghamia lanceolata) through belowground chemical interactions...
September 2016: Ecology
https://www.readbyqxmd.com/read/27852964/spinal-astrocytic-activation-contributes-to-mechanical-allodynia-in-a-rat-model-of-cyclophosphamide-induced-cystitis
#12
Bolong Liu, Minzhi Su, ShaoJun Tang, Xiangfu Zhou, Hailun Zhan, Fei Yang, Wenbiao Li, Tengcheng Li, Juncong Xie
BACKGROUND: Previous studies have demonstrated that glial cells play an important role in the generation and maintenance of neuropathic pain. Activated glial cells produce numerous mediators such as proinflammatory cytokines that facilitate neuronal activity and synaptic plasticity. Similarly, bladder pain syndrome/interstitial cystitis shares many characteristics of neuropathic pain. However, related report on the involvement of spinal glia in bladder pain syndrome/interstitial cystitis-associated pathological pain and the underlying mechanisms are still lacking...
2016: Molecular Pain
https://www.readbyqxmd.com/read/27834499/-i-had-a-sort-of-epiphany-an-exploratory-study-of-group-mindfulness-based-cognitive-therapy-for-older-people-with-depression
#13
Caroline M Williams, Frances Meeten, Susan Whiting
OBJECTIVES: Mindfulness-based cognitive therapy (MBCT) has been successful in reducing depressive symptoms in people with chronic-recurrent depression. However, the research evaluating the efficacy of this approach, and other innovative treatments for mood disorders, has mainly been with people under 65 years. This paper aims to help redress this imbalance by exploring older people's own reflections of their experience of MBCT. METHODS: A qualitative approach was used to explore 13 participants' experiences of MBCT; participants were interviewed pre and post-intervention and again after six months...
November 11, 2016: Aging & Mental Health
https://www.readbyqxmd.com/read/27833274/effect-of-external-volume-expansion-on-the-survival-of-fat-grafts
#14
Raghuveer Reddy, Subramania Iyer, Mohit Sharma, Sundeep Vijayaraghavan, P Kishore, Jimmy Mathew, A K K Unni, P Reshmi, Rakesh Sharma, Chaya Prasad
INTRODUCTION: External volume expansion (EVE) is one method, which has been utilised for increasing the survival of adipose tissue grafts. EVE releases positive pressure from the graft and also induces intense levels of edema that decreases diffusion of metabolites essential for graft survival initially. The ideal timing of external volume expansion in relation to the injection of the fat to facilitate survival is not yet clear. AIMS AND OBJECTIVES: This study was undertaken to evaluate and compare the efficacy of external volume expansion applied at variable time points in relation to the injection of the fat...
May 2016: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/27806306/in%C3%A2-vivo-interrogation-of-spinal-mechanosensory-circuits
#15
Amelia J Christensen, Shrivats M Iyer, Amaury François, Saurabh Vyas, Charu Ramakrishnan, Sam Vesuna, Karl Deisseroth, Grégory Scherrer, Scott L Delp
Spinal dorsal horn circuits receive, process, and transmit somatosensory information. To understand how specific components of these circuits contribute to behavior, it is critical to be able to directly modulate their activity in unanesthetized in vivo conditions. Here, we develop experimental tools that enable optogenetic control of spinal circuitry in freely moving mice using commonly available materials. We use these tools to examine mechanosensory processing in the spinal cord and observe that optogenetic activation of somatostatin-positive interneurons facilitates both mechanosensory and itch-related behavior, while reversible chemogenetic inhibition of these neurons suppresses mechanosensation...
November 1, 2016: Cell Reports
https://www.readbyqxmd.com/read/27802558/does-replacing-live-demonstration-with-instructional-videos-improve-student-satisfaction-and-osteopathic-manipulative-treatment-examination-performance
#16
Ryan Seals, Sharon M Gustowski, Carol Kominski, Feiming Li
CONTEXT: Instructional videos for osteopathic manipulative treatment (OMT) are a potentially valuable resource for novice learners. OBJECTIVE: To evaluate student experiences and the effectiveness of instructional videos in lieu of live faculty demonstration in a second-year osteopathic manipulative medicine course. METHODS: Faculty created and produced written instructions and videos for selected Still and facilitated positional release techniques...
November 1, 2016: Journal of the American Osteopathic Association
https://www.readbyqxmd.com/read/27798311/eradicating-infectious-disease-using-weakly-transmissible-vaccines
#17
Scott L Nuismer, Benjamin M Althouse, Ryan May, James J Bull, Sean P Stromberg, Rustom Antia
Viral vaccines have had remarkable positive impacts on human health as well as the health of domestic animal populations. Despite impressive vaccine successes, however, many infectious diseases cannot yet be efficiently controlled or eradicated through vaccination, often because it is impossible to vaccinate a sufficient proportion of the population. Recent advances in molecular biology suggest that the centuries-old method of individual-based vaccine delivery may be on the cusp of a major revolution. Specifically, genetic engineering brings to life the possibility of a live, transmissible vaccine...
October 26, 2016: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/27762892/evaluating-the-past-present-and-future-of-regenerative-medicine-a-global-view
#18
Vincent Ronfard, Alain A Vertès, Michael H May, Anne Dupraz, Mark E van Dyke, Yves Bayon
"Evaluating the Past and Present of Regenerative Medicine (RM)" was the first part of an Industry Symposium dedicated to the subject during the 2015 TERMIS World Congress in Boston. This working session presented a critical review of the current RM landscape in Europe and North America with possible projections for the future. Interestingly, the RM development cycle seems to obey the Gartner hype cycle, now at the enlightenment phase, after past exaggerated expectations and discouragements, as suggested by increasing numbers of clinical trials and recent market approvals of RM solutions in both Europe (Glybera and Holoclar(®) from Chiesi Pharma and Strimvelis(®) from GSK) and Japan (Remestemcel-L from Mesoblast(®))...
November 21, 2016: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/27758876/exosomes-promote-ovarian-cancer-cell-invasion-through-transfer-of-cd44-to-peritoneal-mesothelial-cells
#19
Koji Nakamura, Kenjiro Sawada, Yasuto Kinose, Akihiko Yoshimura, Aska Toda, Erika Nakatsuka, Kae Hashimoto, Seiji Mabuchi, Ken-Ichirou Morishige, Hirohisa Kurachi, Ernst Lengyel, Tadashi Kimura
: Epithelial ovarian cancer (EOC) cells metastasize within the peritoneal cavity and directly encounter human peritoneal mesothelial cells (HPMCs) as the initial step of metastasis. The contact between ovarian cancer cells and the single layer of mesothelial cells involves direct communications that modulate cancer progression but the mechanisms are unclear. One candidate mediating cell-cell communications are exosomes, 30-100 nm membrane vesicles of endocytic origin, through the cell-cell transfer of proteins, mRNAs, or microRNAs...
October 6, 2016: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/27740595/how-glutamate-is-managed-by-the-blood-brain-barrier
#20
Richard A Hawkins, Juan R Viña
A facilitative transport system exists on the blood-brain barrier (BBB) that has been tacitly assumed to be a path for glutamate entry to the brain. However, glutamate is a non-essential amino acid whose brain content is much greater than plasma, and studies in vivo show that glutamate does not enter the brain in appreciable quantities except in those small regions with fenestrated capillaries (circumventricular organs). The situation became understandable when luminal (blood facing) and abluminal (brain facing) membranes were isolated and studied separately...
October 8, 2016: Biology
keyword
keyword
94863
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"