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https://www.readbyqxmd.com/read/28344018/enhanced-topical-penetration-system-exposure-and-anti-psoriasis-activity-of-two-particle-sized-curcumin-loaded-plga-nanoparticles-in-hydrogel
#1
Lin Sun, Zeyu Liu, Lun Wang, Dongmei Cun, Henry H Y Tong, Ru Yan, Xin Chen, Ruibing Wang, Ying Zheng
Psoriasis is an immune-mediated skin disorder, which is triggered by the aberrant activation of dendritic cells in skin. This activation is followed by the complex interaction between the immune cells in the skin and keratinocyte in the epidermis. To improve the conditions of poor aqueous solubility and chemical stability, overcome skin barriers, and enhance in-vivo anti-psoriatic activity, curcumin (Cur) loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) were fabricated and administered by topical route to treat Imiquimod (IMQ)-induced psoriasis-like mouse model...
March 23, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28341149/docetaxel-loaded-ripl-peptide-iplvvplrrrrrrrrc-conjugated-liposomes-drug-release-cytotoxicity-and-antitumor-efficacy
#2
Ho Yub Yoon, Seong Shin Kwak, Moon Ho Jang, Min Hyung Kang, Si Woo Sung, Chang Hyun Kim, Sung Rae Kim, Dong Woo Yeom, Myung Joo Kang, Young Wook Choi
We previously synthesized the RIPL peptide (IPLVVPLRRRRRRRRC) to facilitate selective delivery into hepsin-expressing cancer cells and showed that RIPL peptide-conjugated liposomes (RIPL-L) enhanced the intracellular delivery of fluorescent probes in vitro. In this study, docetaxel-loaded RIPL-L (DTX-RIPL-L) were prepared and evaluated for in vitro drug release, cytotoxicity, and in vivo antitumor efficacy. DTX was successfully encapsulated by pre-loading, with an average encapsulation efficiency and drug loading capacity of 32...
March 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28334931/rgf1p-rho1p-gef-is-required-for-double-strand-break-repair-in-fission-yeast
#3
Elvira Manjón, Tomás Edreira, Sofía Muñoz, Yolanda Sánchez
Rho GTPases are conserved molecules that control cytoskeletal dynamics. These functions are expedited by Rho GEFs that stimulate the release of GDP to enable GTP binding, thereby allowing Rho proteins to initiate intracellular signaling. How Rho GEFs and Rho GTPases protect cells from DNA damage is unknown. Here, we explore the extreme sensitivity of a deletion mutation in the Rho1p exchange factor Rgf1p to the DNA break/inducing antibiotic phleomycin (Phl). The Rgf1p mutant cells are defective in reentry into the cell cycle following the induction of severe DNA damage...
March 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28332333/allo-hla-cross-reactivities-of-cmv-flu-and-vzv-specific-memory-t-cells-are-shared-by-different-individuals
#4
H van den Heuvel, K M Heutinck, E M W van der Meer-Prins, S L Yong, P P M C van Miert, J D H Anholts, M E I Franke-van Dijk, X Q Zhang, D L Roelen, R J M Ten Berge, F H J Claas
Virus-specific T cells can recognize allogeneic HLA (allo-HLA) through TCR cross-reactivity. The allospecificity often differs per individual ("private cross-reactivity"), but can also be shared by multiple individuals ("public cross-reactivity"). However, only a few examples of the latter have been described. Since these could facilitate alloreactivity prediction in transplantation, we aimed to identify novel public cross-reactivities of human virus-specific CD8+ T cells directed against allo-HLA by assessing their reactivity in mixed-lymphocyte reactions...
March 23, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28330718/adult-glucocorticoid-receptor-mrna-expression-volatility-in-response-to-an-acute-stressor-and-juvenile-csf-corticotropin-releasing-factor-a-pilot-neurodevelopmental-study
#5
Shariful A Syed, Eric L P Smith, Olcay Batuman, Leonard A Rosenblum, Michael J Owens, Charles B Nemeroff, Jeremy D Coplan
INTRODUCTION: Early life stress (ELS) has been shown to play a role in establishing persistent maladaptive HPA axis modifications that may contribute to the pathogenesis of mood and anxiety disorders. Central glucocorticoid receptor (GR) messenger RNA (mRNA) expression may facilitate (mal)adaptive responsivity to ELS. The role of adult monocytic GR mRNA expression, a putative CNS proxy, during acute stress exposure was explored as well as the ELS marker, juvenile cerebrospinal fluid (CSF) corticotropin-releasing factor...
March 19, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28330134/phytases-from-enterobacter-and-serratia-species-with-desirable-characteristics-for-food-and-feed-applications
#6
Harpreet Kaur Kalsi, Rajveer Singh, Harcharan Singh Dhaliwal, Vinod Kumar
Phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. These catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. In the present investigation, 43 phytase positive bacteria on PSM plates were isolated from different sources and characterized for phytase activity...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28325685/a-positive-feedback-strategy-for-enhanced-chemotherapy-based-on-ros-triggered-self-accelerating-drug-release-nanosystem
#7
Jing-Jing Hu, Qi Lei, Meng-Yun Peng, Di-Wei Zheng, Yi-Xuan Chen, Xian-Zheng Zhang
Here, a positive feedback strategy was utilized to amplify the concentration of intracellular reactive oxygen species (ROS) and a ROS-triggered self-accelerating drug release nanosystem (defined as T/D@RSMSNs) was demonstrated for enhanced tumor chemotherapy. The mesoporous silica nanoparticles (MSNs) based nanocarriers were gated by β-cyclodextrin (β-CD) through the ROS-cleavable thioketal (TK) linker to encapsulate the anticancer drug doxorubicin hydrochloride (DOX) and ROS producing agent α-tocopheryl succinate (α-TOS), whose surface was further anchored with adamantane conjugated poly(ethylene glycol) chain (AD-PEG) via host-guest interaction...
March 11, 2017: Biomaterials
https://www.readbyqxmd.com/read/28319867/phototherapeutic-spectrum-expansion-through-synergistic-effect-of-mesoporous-silica-trio-nanohybrids-against-antibiotic-resistant-gram-negative-bacterium
#8
Yaswanth Kuthati, Ranjith Kumar Kankala, Prabhakar Busa, Shi-Xiang Lin, Jin-Pei Deng, Chung-Yuan Mou, Chia-Hung Lee
The extensive impact of antibiotic resistance has led to the exploration of new anti-bacterial modalities. We designed copper impregnated mesoporous silica nanoparticles (Cu-MSN) with immobilizing silver nanoparticles (SNPs) to apply photodynamic inactivation (PDI) of antibiotic-resistant E. coli. SNPs were decorated over the Cu-MSN surfaces by coordination of silver ions on diamine-functionalized Cu-MSN and further reduced to silver nanoparticles with formalin. We demonstrate that silver is capable of sensitizing the gram-negative bacteria E...
March 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28288210/depletion-of-neat1-lncrna-attenuates-nucleolar-stress-by-releasing-sequestered-p54nrb-and-psf-to-facilitate-c-myc-translation
#9
Wen Shen, Xue-Hai Liang, Hong Sun, Cheryl L De Hoyos, Stanley T Crooke
Altered expression of NEAT1, the architectural long non-coding RNA (lncRNA) of nuclear paraspeckles, has been reported during tumorigenesis, as well as under various cellular stress conditions. Here we report that the depletion of NEAT1 lncRNA alleviates nucleolar stress during RNAP I inhibition through releasing sequestered P54nrb and PSF to facilitate the IRES-dependent translation of c-Myc. RNAP I inhibitor CX5461 disrupts the SL1-rDNA interaction and induces nucleolar disruption, demonstrated by the accumulation of fibrillarin-containing nucleoplasmic foci and nucleolar clearance of ribosomal proteins in HeLa cells...
2017: PloS One
https://www.readbyqxmd.com/read/28280844/in-depth-analyses-of-native-n-linked-glycans-facilitated-by-high-performance-anion-exchange-chromatography-pulsed-amperometric-detection-coupled-to-mass-spectrometry
#10
Zoltan Szabo, James R Thayer, Yury Agroskin, Shanhua Lin, Yan Liu, Kannan Srinivasan, Julian Saba, Rosa Viner, Andreas Huhmer, Jeff Rohrer, Dietmar Reusch, Rania Harfouche, Shaheer H Khan, Christopher Pohl
Characterization of glycans present on glycoproteins has become of increasing importance due to their biological implications, such as protein folding, immunogenicity, cell-cell adhesion, clearance, receptor interactions, etc. In this study, the resolving power of high-performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) was applied to glycan separations and coupled to mass spectrometry to characterize native glycans released from different glycoproteins. A new, rapid workflow generates glycans from 200 μg of glycoprotein supporting reliable and reproducible annotation by mass spectrometry (MS)...
March 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28279755/purinergic-p2-receptors-in-the-paraventricular-nucleus-of-the-hypothalamus-are-involved-in-hyperosmotic-induced-sympathoexcitation
#11
H C Ferreira-Neto, I M R Ribeiro, T S Moreira, S T Yao, V R Antunes
Increases in plasma osmolality activates the paraventricular nucleus of the hypothalamus (PVN) which in turn mounts a physiological response by increasing the release of arginine vasopressin and sympathetic nerve activity to end organs such as the kidney. The PVN expresses an abundance of purinergic receptors including P2X2 receptors. In the present study, we sought to determine (1) whether P2X2-expressing PVN neurons are activated by hypertonic saline or hypertonic mannitol and (2) what effects P2X receptor blockade has on sympathetic nerve activation mediated by a hyperosmotic stimulus...
March 6, 2017: Neuroscience
https://www.readbyqxmd.com/read/28267620/cancer-associated-circulating-large-extracellular-vesicles-in-cholangiocarcinoma-and-hepatocellular-carcinoma
#12
Henrike Julich-Haertel, Sabine K Urban, Marcin Krawczyk, Arnulf Willms, Krzysztof Jankowski, Waldemar Patkowski, Beata Kruk, Maciej Krasnodębski, Joanna Ligocka, Robert Schwab, Ines Richardsen, Sebastian Schaaf, Angelina Klein, Sebastian Gehlert, Hanna Sänger, Markus Casper, Jesus M Banales, Detlef Schuppan, Piotr Milkiewicz, Frank Lammert, Marek Krawczyk, Veronika Lukacs-Kornek, Miroslaw Kornek
BACKGROUND AND AIMS: we previously reported that large extracellular vesicles, specifically AnnexinV(+)EpCAM(+)CD147(+) tumour-associated microparticles (taMPs), facilitate the detection of colorectal carcinoma (CRC), non-small cell lung carcinoma (NSCLC) as well as pancreas carcinoma (PaCa). Here we assess the diagnostic value of taMPs for detection and monitoring of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA). Specifically, we aim to differentiate liver taMPs from other cancer taMPs, such as CRC and NSCLC...
March 3, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28266827/incorporation-of-human-platelet-derived-growth-factor-bb-encapsulated-poly-lactic-co-glycolic-acid-microspheres-into-3d-coragraf-enhances-osteogenic-differentiation-of-mesenchymal-stromal-cells
#13
Saktiswaren Mohan, Hanumantharao Balaji Raghavendran, Puvanan Karunanithi, Malliga Raman Murali, Sangeetha Vasudevaraj Naveen, Sepehr Talebian, Mohammad Mehrali, Mehdi Mehrali, Elango Natarajan, Chee Ken Chan, Tunku Kamarul
Tissue engineering aims to generate or facilitate regrowth or healing of damaged tissues by applying a combination of biomaterials, cells, and bioactive signaling molecules. In this regard, growth factors clearly play important roles in regulating cellular fate. However, uncontrolled release of growth factors has been demonstrated to produce severe side effects on the surrounding tissues. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres (MS) incorporated three-dimensional (3D) CORAGRAF scaffolds were engineered to achieve controlled release of platelet-derived growth factor-BB (PDGF-BB) for the differentiation of stem cells within the 3D polymer network...
March 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28263813/ligand-decorated-click-polypeptide-derived-nanoparticles-for-targeted-drug-delivery-applications
#14
Mohiuddin A Quadir, Stephen W Morton, Lawrence B Mensah, Kevin Shopsowitz, Jeroen Dobbelaar, Nicole Effenberger, Paula T Hammond
A ligand decorated, synthetic polypeptide block copolymer platform with environment-responsive capabilities was designed. We evaluated the potential of this system to function as a polymersome for targeted-delivery of a systemic chemotherapy to tumors. Our system employed click chemistry to provide a pH-responsive polypeptide block that drives nanoparticle assembly, and a ligand (folic acid) conjugated PEG block that targets folate-receptor over-expressing cancer cells. These nanocarriers were found to encapsulate a high loading of conventional chemotherapeutics (e...
March 2, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28263585/acute-responses-of-microorganisms-from-membrane-bioreactors-in-the-presence-of-naocl-protective-mechanisms-of-extracellular-polymeric-substances
#15
Xiaomeng Han, Zhiwei Wang, Mei Chen, Xingran Zhang, Chuyang Y Tang, Zhichao Wu
Extracellular polymeric substances (EPS) are key foulants in membrane bioreactors (MBRs). However, their positive functions of protecting microorganisms from environmental stresses, e.g., during in situ hypochlorite chemical cleaning of membranes, have not been adequately elucidated. In this work, we investigated the response of microorganisms in an MBR to various dosages of NaOCl, with a particular emphasis on the mechanistic roles of EPS. Results showed that functional groups in EPS such as the hydroxyl and amino groups were attacked by NaOCl, causing the oxidation of polysaccharides, denaturation of amino acids, damage to protein secondary structure, and transformation of tryptophan protein-like substances to condensed aromatic ring substances...
March 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28260798/geniposide-ameliorates-tnbs-induced-experimental-colitis-in-rats-via-reducing-inflammatory-cytokine-release-and-restoring-impaired-intestinal-barrier-function
#16
Bin Xu, Yan-Li Li, Ming Xu, Chang-Chun Yu, Meng-Qiao Lian, Ze-Yao Tang, Chuan-Xun Li, Yuan Lin
Geniposide is an iridoid glycosides purified from the fruit of Gardenia jasminoides Ellis, which is known to have antiinflammatory, anti-oxidative and anti-tumor activities. The present study aimed to investigate the effects of geniposide on experimental rat colitis and to reveal the related mechanisms. Experimental rat colitis was induced by rectal administration of a TNBS solution. The rats were treated with geniposide (25, 50 mg·kg(-1)·d(-1), ig) or with sulfasalazine (SASP, 100 mg·kg(-1)·d(-1), ig) as positive control for 14 consecutive days...
March 6, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28236789/ph-and-redox-dual-responsive-multifunctional-gene-delivery-with-enhanced-capability-of-transporting-dna-into-the-nucleus
#17
Zhe Yang, Yingqin Li, Jinbiao Gao, Zhong Cao, Qing Jiang, Jie Liu
Stimuli-responsive gene delivery vectors based on physiologically triggered structure changing have been recently recognized as a new therapeutic agent for their excellent performance in vivo. Herein, we present an intelligent gene delivery system based on the octa-arginine peptides (R8)-conjugated polyamino acid derivatives noted as PPCRC (PVIm-(PAsp-Cystamine-R8)-Cholesteryl), which processed pH responsive, surface charge-switching, intracellular redox-responsive and enhanced nucleus import of gene together...
February 16, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28235576/lidocaine-multivalent-ion-complex-as-a-potential-strategy-for-prolonged-local-anesthesia
#18
You Jin Jang, Jin Ho Lee, Tae Beom Seo, Se Heang Oh
Local anesthetics are adopted in clinical practice to manage the perioperative and/or postoperative pain. However, their relatively short duration of action limit their ability to meet clinical needs. Herein, we prepared a lidocaine/multivalent ion complex (icLD) using aqueous solutions containing positively charged LD and a multivalent counter-ion as a system for producing prolonged anesthesia. The results of the in vitro and in vivo experiments indicated that the icLD facilitates prolonged LD release even without adjuvants and thus provides nerve blockade for a long duration of action (∼14hrs) without further increase in neurotoxicity than the LD itself...
February 21, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28225587/compms2miner-an-automatable-metabolite-identification-visualization-and-data-sharing-r-package-for-high-resolution-lc-ms-data-sets
#19
William M B Edmands, Lauren Petrick, Dinesh K Barupal, Augustin Scalbert, Mark J Wilson, Jeffrey K Wickliffe, Stephen M Rappaport
A long-standing challenge of untargeted metabolomic profiling by ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) is efficient transition from unknown mass spectral features to confident metabolite annotations. The compMS(2)Miner (Comprehensive MS(2) Miner) package was developed in the R language to facilitate rapid, comprehensive feature annotation using a peak-picker-output and MS(2) data files as inputs. The number of MS(2) spectra that can be collected during a metabolomic profiling experiment far outweigh the amount of time required for pain-staking manual interpretation; therefore, a degree of software workflow autonomy is required for broad-scale metabolite annotation...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28222701/the-effect-of-deep-muscle-relaxation-on-the-force-required-during-latissimus-dorsi-dissection-for-breast-reconstructive-surgery-results-of-a-prospective-double-blinded-observational-pilot-study
#20
T Ledowski, A Goodwin-Walters, P Quinn, M Calvert
BACKGROUND: The use of neuromuscular blocking agents has previously been suggested to facilitate the dissection of the latissimus dorsi muscle during breast reconstructive surgery. The aim of this study was to quantify the influence of deep muscle relaxation on the force required to lift the latissimus dorsi muscle during flap preparation. METHODS: After ethics approval and written informed consent 15 patients scheduled for elective breast reconstruction with a latissimus dorsi pedicled flap (muscle flap, not myocutaneous flap) under general anaesthesia were prospectively included...
February 21, 2017: BMC Anesthesiology
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