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https://www.readbyqxmd.com/read/29724034/an-improved-weighted-partial-least-squares-method-coupled-with-near-infrared-spectroscopy-for-rapid-determination-of-multiple-components-and-anti-oxidant-activity-of-pu-erh-tea
#1
Ze Liu, Hua-Lin Xie, Lin Chen, Jian-Hua Huang
Background: Pu-erh tea is a unique microbially fermented tea, which distinctive chemical constituents and activities are worthy of systematic study. Near infrared spectroscopy (NIR) coupled with suitable chemometrics approaches can rapidly and accurately quantitatively analyze multiple compounds in samples. Methods: In this study, an improved weighted partial least squares (PLS) algorithm combined with near infrared spectroscopy (NIR) was used to construct a fast calibration model for determining four main components, i...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29556381/protective-effect-of-pu-erh-tea-extracts-against-ethanol-induced-gastric-mucosal-damage-in-rats
#2
Jinna Yang, Wangyi Zhou, Yaru Gu, Jinwei Dai, Xinxin Li, Ping Tai, Yanchuan Li, Xiaohui Ma, Yuyang Zhang
Pu-erh tea has become a focus of research due to its reported biological activities, including anti-oxidation, anti-inflammation and anti-immunosenescence. The present study was performed to evaluate the potential gastroprotective function of Pu-erh tea extracts against ethanol-induced gastric mucosal damage in rats. Sprague Dawley rats were randomly divided into seven groups: A normal control, a model control, a cimetidine (0.08 g/kg) group, three Pu-erh tea extracts groups (low, moderate and high-dose; 0...
April 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29493150/-determination-of-mycotoxins-in-pu-erh-tea-black-tea-and-green-tea-samples
#3
Lu Wang, Hua Yang, Guo-Xiang Xie, Wei Jia
To establish a robust method for the determination of mycotoxins in tea samples, and to provide means for the quality and safety control of tea products. Samples of 20 tea products acquired from international market were extracted by organic solvents (acetonitrile containing 0.1% formic acid) or hot water, respectively. The extracts were analyzed by UPLC-MS/MS.A good linear regression was achieved in a range of 39.1 to 5 000 ng•L⁻¹ for aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1), 117 to 15 000 ng•L⁻¹ for aflatoxin B2 (AFB2) and aflatoxin G2 (AFG2), 2...
December 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29424141/-adulteration-detection-of-tea-samples-based-on-plant-rbcl-gene-sequencing
#4
Minxuan Wang, Yongjie Zhang
Tea is one of the most popular drinks in the world, but counterfeit or adulterated tea can be found now and then on the tea market. The traditional methods dependent on sensory, physical and chemical tests cannot identify the composition of adulterated plant species accurately. We developed therefore a method for identification of adulterated plants in tea based on qualitative detection of plant rbcL (Ribulose 1,5-bisphosphate carboxylase-oxygenase large subunit) fragments, which involved amplification, sequencing and sequence analyses of rbcL fragments...
February 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29207513/methylxanthine-content-in-commonly-consumed-foods-in-spain-and-determination-of-its-intake-during-consumption
#5
Juan M Sanchez
Methylxanthines present psychostimulant effects. These compounds have low toxicity and their consumption at moderate levels presents some beneficial health effects, whereas some significant risk appears at high levels. Samples of common types of methylxanthine-containing beverages and foods consumed in Spain were analyzed to determine their content. Caffeine was the methylxanthine that was most found in the samples investigated. Instant coffees gave the highest caffeine percentage (18-44 mg·g-1 ). Green and scented teas were found to have a caffeine dry-weight content (8-26 mg·g-1 ) equivalent to ground coffees (13-23 mg·g-1 ), but black and pu-erh teas (18-30 mg·g-1 ) had a higher caffeine content...
December 4, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29069630/effects-of-pu-erh-ripened-tea-on-hyperuricemic-mice-studied-by-serum-metabolomics
#6
Ran Zhao, Dong Chen, Hualing Wu
To evaluate effects of Pu-erh ripened tea in hyperuricemic mice, a mouse hyperuricemia model was developed by oral administration of potassium oxonate for 7 d. Serum metabolomics, based on gas chromatography-mass spectrometry, was used to generate metabolic profiles from normal control, hyperuricemic and allopurinol-treated hyperuricemic mice, as well as hyperuricemic mice given Pu-erh ripened tea at three doses. Pu-erh ripened tea significantly lowered serum uric acid levels. Twelve potential biomarkers associated with hyperuricemia were identified...
November 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28964372/phytochemical-profiles-and-antioxidant-activities-of-chinese-dark-teas-obtained-by-different-processing-technologies
#7
Hai-Peng Lv, Yue Zhang, Jiang Shi, Zhi Lin
Dark teas are rich in secondary metabolites, such as phenolics and flavonoids, which have been suggested to be associated with their health benefits. In this study, the concentrations of tea polyphenols, tea pigments, catechins, flavonoids, alkaloid, and volatile components in 44 dark tea samples, including Pu-erh, Fuzhuan and Liubao teas, were systematically examined. Among the samples tested, Pu-erh tea contained the highest total flavonoid content (5.24±0.05%), followed by Liubao (4.45±0.61%) and Fuzhuan teas (3...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28822144/-progress-in-research-for-pharmacological-effects-of-pu-erh-tea
#8
REVIEW
Xiao-Pan Gu, Bo Pan, Zhen Wu, Yun-Fang Zhao, Peng-Fei Tu, Jiao Zheng
Pu-erh tea has gradually aroused general concern with social development and people's enhanced awareness of health. Pu-erh tea is rich in multiple active constitute such as flavonoids, catechins, phenolic acids, flavanols polymer, purine alkaloids, and hydrolysable tannin as a microbial-fermented tea.It is reported that Pu-erh tea have a variety of pharmacologically activities, such as anti-hyperlipidemic, anti-diabetic, anti-oxidative, anti-tumor, anti-bacterial, anti-inflammatory, and anti-viral effects. In this paper, the main pharmacological effects of Pu-erh tea are reviewed...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28792381/effects-of-tea-extracts-on-the-colonization-behaviour-of-candida-species-attachment-inhibition-and-biofilm-enhancement
#9
Yi Wang, H M H N Bandara, Deirdre Mikkelsen, Lakshman P Samaranayake
PURPOSE: We assessed the effects of four different types of tea extracts (green, oolong, black and pu-erh tea) on cellular surface properties (hydrophobicity and auto-aggregation) and the colonization attributes (attachment and biofilm formation) of four strains of Candida albicans and three strains of Candida krusei. METHODOLOGY: The cellular surface properties were determined using spectrophotometry. The colonization activities were quantified using colorimetric viability assays and visualized using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM)...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28620304/pu-erh-tea-extract-ameliorates-ovariectomy-induced-osteoporosis-in-rats-and-suppresses-osteoclastogenesis-in-vitro
#10
Titi Liu, Shihua Ding, Dan Yin, Xiangdan Cuan, Chuanqi Xie, Huanhuan Xu, Xuanjun Wang, Jun Sheng
Background and Objective: Tea drinking is associated with positive effects on bone health and may protect against osteoporosis, especially in elderly women. Pu-erh tea has many beneficial effects on human health; however, whether Pu-erh tea has anti-osteoporotic potential remains unclear. Thus, we investigated the effects of Pu-erh tea extract (PTE) on ovariectomy-induced osteoporosis in rats and on osteoclastogenesis in vitro. Methods: Female Wistar rats were divided into six groups: the sham, model, and Xian-Ling-Gu-Bao capsule (XLGB) groups, and the low-, medium-, and high-dose PTE groups...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28540171/flavanols-from-the-camellia-sinensis-var-assamica-and-their-hypoglycemic-and-hypolipidemic-activities
#11
Xin Wang, Quan Liu, Hongbo Zhu, Hongqing Wang, Jie Kang, Zhufang Shen, Ruoyun Chen
α-Glucosidase and lipase inhibitors play important roles in the treatment of hyperglycaemia and dyslipidemia. To identify novel naturally occurring inhibitors, a bioactivity-guided phytochemical research was performed on the pu-erh tea. One new flavanol, named (-)-epicatechin-3-O-(Z)-coumarate (1), and 16 known analogs (2-17) were isolated from the aqueous extract of the pu-erh tea. Their structures were determined by spectroscopic and chemical methods. Furthermore, the water extract of pu-erh tea and its fractions exhibited inhibitory activities against α-glucosidases and lipases in vitro; compound 15 showed moderate inhibitory effect against sucrase with an IC50 value of 32...
May 2017: Acta Pharmaceutica Sinica. B
https://www.readbyqxmd.com/read/28428754/pu-erh-tea-water-extract-mediates-cell-cycle-arrest-and-apoptosis-in-mda-mb-231-human-breast-cancer-cells
#12
Jing Xie, Haishuang Yu, Shuang Song, Chongye Fang, Xuanjun Wang, Zhongbin Bai, Xiao Ma, Shumei Hao, Hong-Ye Zhao, Jun Sheng
Pu-erh tea is believed to have health benefits, the growth inhibition activity of Pu-erh tea on breast cancer cell has not been investigated. In this study, we examined the activity of Pu-erh tea water extract on apoptosis and cell cycle arrest in the human breast adenocarcinoma cell line MDA-MB-231 and clarified its underlying mechanism of action. We found that Pu-erh tea extract inhibited cell proliferation and induced apoptosis in a dose-dependent manner. We also found that Pu-erh tea extract inhibited tumor cell growth within 24 h via accumulation of cells in S phase...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28410522/development-of-matrix-effect-free-mispe-uhplc-ms-ms-method-for-determination-of-lovastatin-in-pu-erh-tea-oyster-mushroom-and-red-yeast-rice
#13
Pavel Svoboda, Daniel Sander, Kateřina Plachká, Lucie Nováková
Matrix effect-free UHPLC-MS/MS method was developed and validated for the determination of cholesterol-lowering lovastatin in food samples represented by Pu-erh tea, oyster mushroom, and red yeast rice. The resulting method was fully validated in terms of intra-day and inter-day precision, accuracy, linearity, range, LOD, LOQ, and matrix effects. The matrix effect phenomenon evaluated by comparison of slopes of calibration curves was completely eliminated by solid-phase extraction based on the technique of molecularly imprinted polymers (MIPs)...
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28399674/optimization-and-validation-of-folin-ciocalteu-method-for-the-determination-of-total-polyphenol-content-of-pu-erh-tea
#14
Marilena Musci, Shicong Yao
Pu-erh tea is a post-fermented tea that has recently gained popularity worldwide, due to potential health benefits related to the antioxidant activity resulting from its high polyphenolic content. The Folin-Ciocalteu method is a simple, rapid, and inexpensive assay widely applied for the determination of total polyphenol content. Over the past years, it has been subjected to many modifications, often without any systematic optimization or validation. In our study, we sought to optimize the Folin-Ciocalteu method, evaluate quality parameters including linearity, precision and stability, and then apply the optimized model to determine the total polyphenol content of 57 Chinese teas, including green tea, aged and ripened Pu-erh tea...
December 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28164196/preventive-effect-of-silibinin-in-combination-with-pu-erh-tea-extract-on-non-alcoholic-fatty-liver-disease-in-ob-ob-mice
#15
Wen-Yi Hu, Xiao-Hui Ma, Wang-Yi Zhou, Xin-Xin Li, Ting-Ting Sun, He Sun
There are presently no miracle drugs for non-alcoholic fatty liver disease (NAFLD). This study investigates the synergistic effect of Silibinin combined with Pu-erh tea extract (PTE) against NAFLD and explores the suggested mechanism of action. Ob/ob mice were fed a high fat diet along with the oral administration of Silibinin (86 mg per kg per day), PTE (250 mg per kg per day) or their combination for 6 weeks. Their lean littermates who were fed with standard chow diet were used as the control group. The blood biochemical index and histopathological evaluation were analyzed...
March 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28154484/analysis-of-the-mat1-1-and-mat1-2-gene-ratio-in-black-koji-molds-isolated-from-meju
#16
Anbazhagan Mageswari, Jeong-Seon Kim, Kyu-Ho Cheon, Soon-Wo Kwon, Osamu Yamada, Seung-Beom Hong
Aspergillus luchuensis is known as an industrially important fungal species used for making fermented foods such as awamori and shochu in Japan, makgeolli and Meju in Korea, and Pu-erh tea in China. Nonetheless, this species has not yet been widely studied regarding mating-type genes. In this study, we examined the MAT1-1 and MAT1-2 gene ratio in black koji molds (A. luchuensis, Aspergillus niger, and Aspergillus tubingensis) and in Aspergillus welwitschiae isolated from Meju, a fermented soybean starting material for traditional soy sauce and soybean paste in Korea...
December 2016: Mycobiology
https://www.readbyqxmd.com/read/27763516/transcriptome-profile-reveals-that-pu-erh-tea-represses-the-expression-of-vitellogenin-family-to-reduce-fat-accumulation-in-caenorhabditis-elegans
#17
Ru-Yue Xiao, Junjun Hao, Yi-Hong Ding, Yan-Yun Che, Xiao-Ju Zou, Bin Liang
Due to misbalanced energy surplus and expenditure, obesity has become a common chronic disorder that is highly associated with many metabolic diseases. Pu-erh tea, a traditional Chinese beverage, has been believed to have numerous health benefits, such as anti-obesity. However, the underlying mechanisms of its anti-obesity effect are yet to be understood. Here, we take the advantages of transcriptional profile by RNA sequencing (RNA-Seq) to view the global gene expression of Pu-erh tea. The model organism Caenorhabditis elegans was treated with different concentrations of Pu-erh tea water extract (PTE, 0 g/mL, 0...
October 17, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27754346/gallic-acid-content-in-taiwanese-teas-at-different-degrees-of-fermentation-and-its-antioxidant-activity-by-inhibiting-pkc%C3%AE-activation-in-vitro-and-in-silico-studies
#18
Teeradate Kongpichitchoke, Ming-Tzu Chiu, Tzou-Chi Huang, Jue-Liang Hsu
Teas can be classified according to their degree of fermentation, which has been reported to affect both the bioactive components in the teas and their antioxidative activity. In this study, four kinds of commercial Taiwanese tea at different degrees of fermentation, which include green (non-fermented), oolong (semi-fermented), black (fully fermented), and Pu-erh (post-fermented) tea, were profiled for catechin levels by using high performance liquid chromatography (HPLC). The result indicated that the gallic acid content in tea was directly proportional to the degree of fermentation in which the lowest and highest gallic acid content were 1...
October 12, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27578019/pu-erh-tea-protects-the-nervous-system-by-inhibiting-the-expression-of-metabotropic-glutamate-receptor-5
#19
Chunjie Li, Shaomeng Chai, Yongzhi Ju, Lu Hou, Hang Zhao, Wei Ma, Tian Li, Jun Sheng, Wei Shi
Glutamate is one of the major excitatory neurotransmitters of the CNS and is essential for numerous key neuronal functions. However, excess glutamate causes massive neuronal death and brain damage owing to excitotoxicity via the glutamate receptors. Metabotropic glutamate receptor 5 (mGluR5) is one of the glutamate receptors and represents a promising target for studying neuroprotective agents of potential application in neurodegenerative diseases. Pu-erh tea, a fermented tea, mainly produced in Yunnan province, China, has beneficial effects, including the accommodation of the CNS...
September 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/27373646/theagalloflavic-acid-a-new-pigment-derived-from-hexahydroxydiphenoyl-group-and-lignan-oxidation-products-produced-by-aerobic-microbial-fermentation-of-green-tea
#20
Yosuke Matsuo, Tomoko Matsuda, Keisuke Sugihara, Yoshinori Saito, Ying-Jun Zhang, Chong-Ren Yang, Takashi Tanaka
Chinese ripe pu-erh tea is produced by aerobic microbial fermentation of green tea. To clarify the microbial degradation of tea polyphenols, Japanese commercial green tea was mixed with Chinese ripe pu-erh tea, which retains microorganisms, and fermented for 5 d. Chromatographic separation yielded a novel water-soluble yellow pigment termed theagalloflavic acid. Spectroscopic and chemical evidence suggested that this pigment was produced by oxidative ring cleavage of hexahydroxydiphenoyl esters. In addition, two new oxygenated lignin metabolites, (+)-5,5'-dihydroxypinoresinol and 5-hydroxydihydrodehydrodiconiferyl alcohol, were also isolated together with known degradation products of quercetin and tea catechins...
2016: Chemical & Pharmaceutical Bulletin
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