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https://www.readbyqxmd.com/read/28735521/myd88-erk-nfkb%C3%A2-pathways-and-pro-inflammatory-cytokines-release-in-periodontal-ligament-stem-cells-stimulated-by-porphyromonas%C3%A2-gingivalis
#1
Francesca Diomede, Maria Zingariello, Marcos F X B Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia De Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani
The present study was aimed at investigating whether human Periodontal Ligament Stem Cells (hPDLSCs) were capable of sensing and reacting to lipopolysaccharide from Porphyromonas gingivalis (LPS-G) which is widely recognized as a major pathogen in the development and progression of periodontitis. At this purpose hPDLCs were stimulated with 5 μg/mL LPS-G various times and the expression of toll-like receptor 4 (TLR4) was evaluated. Toll-like receptors (TLRs) play an essential role in innate immune signaling in response to microbial infections, and in particular TLR4, type-I transmembrane proteins, has been shown recognizing LPS-G...
May 24, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735502/regenerative-medicine-in-kidney-disease-where-we-stand-and-where-to-go
#2
REVIEW
Fernanda T Borges, Nestor Schor
The kidney is a complex organ with more than 20 types of specialized cells that play an important role in maintaining the body's homeostasis. The epithelial tubular cell is formed during embryonic development and has little proliferative capacity under physiological conditions, but after acute injury the kidney does have regenerative capacity. However, after repetitive or severe lesions, it may undergo a maladaptation process that predisposes it to chronic kidney injury. Regenerative medicine includes various repair and regeneration techniques, and these have gained increasing attention in the scientific literature...
July 22, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28735492/isolation-of-biologically-active-exosomes-from-plasma-of-patients-with-cancer
#3
Chang-Sook Hong, Sonja Funk, Theresa L Whiteside
A method for exosome isolation from human plasma was developed for rapid, high-throughput processing of plasma specimens obtained from patients with cancer. This method removes the bulk of plasma proteins associated with exosomes and can be used for comparative examinations of exosomes and their content in serial specimens of patients' plasma, allowing for monitoring changes in exosome numbers, profiles, and functions in the course of cancer progression or during therapy. The plasma-derived exosomes can be recovered in quantities sufficient for the characterization of their morphology by transmission electron microscopy (TEM), size and concentration by qNano, protein/lipid ratios, nucleic acid extraction, molecular profiling by Western blots or immune arrays, and functional assays...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735489/a-zebrafish-model-for-evaluating-the-function-of-human-leukemic-gene-idh1-and-its-mutation
#4
Alvin C H Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, Anskar Y H Leung
The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735360/navigating-the-future-of-cardiovascular-drug-development-leveraging-novel-approaches-to-drive-innovation-and-drug-discovery-summary-of-findings-from-the-novel-cardiovascular-therapeutics-conference
#5
REVIEW
Thomas J Povsic, Rob Scott, Kenneth W Mahaffey, Robert Blaustein, Jay M Edelberg, Martin P Lefkowitz, Scott D Solomon, Jonathan C Fox, Kevin E Healy, Aarif Y Khakoo, Douglas W Losordo, Fady I Malik, Brett P Monia, Rusty L Montgomery, Jeffrey Riesmeyer, Gregory G Schwartz, Steven L Zelenkofske, Joseph C Wu, Scott M Wasserman, Matthew T Roe
PURPOSE: The need for novel approaches to cardiovascular drug development served as the impetus to convene an open meeting of experts from the pharmaceutical industry and academia to assess the challenges and develop solutions for drug discovery in cardiovascular disease. METHODS: The Novel Cardiovascular Therapeutics Summit first reviewed recent examples of ongoing or recently completed programs translating basic science observations to targeted drug development, highlighting successes (protein convertase sutilisin/kexin type 9 [PCSK9] and neprilysin inhibition) and targets still under evaluation (cholesteryl ester transfer protein [CETP] inhibition), with the hope of gleaning key lessons to successful drug development in the current era...
July 22, 2017: Cardiovascular Drugs and Therapy
https://www.readbyqxmd.com/read/28735348/analysis-of-the-cd8-t-cell-anti-hiv-activity-in-heterologous-cell-co-cultures-reveals-the-benefit-of-multiple-hla-class-i-matches
#6
M Scott Killian, Fernando Teque, Ramu Sudhagoni
CD8(+) T lymphocytes can reduce the production of human immunodeficiency virus 1 (HIV-1) by CD4(+) T cells by cytotoxic and non-cytotoxic mechanisms. To investigate the involvement of human leukocyte antigen (HLA) class I compatibility in anti-HIV responses, we co-cultured primary CD8(+) T cells, isolated from the peripheral blood of HIV-1-infected individuals, with panels of autologous and heterologous acutely HIV-1-infected primary CD4(+) T cells. Altogether, CD8(+) T cell anti-HIV activity was evaluated in more than 200 co-cultures...
July 22, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28735242/transcriptomic-analysis-of-the-larva-taenia-multiceps
#7
W H Li, N Z Zhang, L Yue, Y Yang, L Li, H B Yan, T T Li, Z G Qu, W Z Jia, B Q Fu
Taenia multiceps is an adult worm affiliated to Taeniidae family, Platyhelminthes phylum. The larvae of the parasite (Coenurus cerebralis) parasitic in the brain and spinal cord in domestic and wild ruminants or humans can led to a fatal central nervous system (CNS) disease. The aims of the present study were to define the transcriptome profiles of the larvae of T. multiceps by RNA-Seq approach, and to generate large functional gene datasets that could be used to predict the key molecular pathways linked to this cestode...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28735231/exploring-the-association-between-heat-and-mortality-in-switzerland-between-1995-and-2013
#8
Martina S Ragettli, Ana M Vicedo-Cabrera, Christian Schindler, Martin Röösli
Designing effective public health strategies to prevent adverse health effect of hot weather is crucial in the context of global warming. In Switzerland, the 2003 heat have caused an estimated 7% increase in all-cause mortality. As a consequence, the Swiss Federal Office of Public Health developed an information campaign to raise public awareness on heat threats. For a better understanding on how hot weather affects daily mortality in Switzerland, we assessed the effect of heat on daily mortality in eight Swiss cities and population subgroups from 1995 to 2013 using different temperature metrics (daily mean (Tmean), maximum (Tmax), minimum (Tmin) and maximum apparent temperature (Tappmax)), and aimed to evaluate variations of the heat effect after 2003 (1995-2002 versus 2004-2013)...
July 20, 2017: Environmental Research
https://www.readbyqxmd.com/read/28735230/granulometry-microbial-composition-and-biological-activity-of-dusts-collected-in-french-dairy-farms
#9
Hugo Pfister, Claudie Morzadec, Pierre Le Cann, Laurent Madec, Valérie Lecureur, Martine Chouvet, Stéphane Jouneau, Laurent Vernhet
BACKGROUND: Dairy working increases the prevalence of lower airway respiratory diseases, especially COPD and asthma. Epidemiological studies have reported that chronic inhalation of organic dusts released during specific daily tasks could represent a major risk factor for development of these pathologies in dairy workers. Knowledge on size, nature and biological activity of such organic dusts remain however limited. OBJECTIVE: To compare size distribution, microbial composition and cellular effects of dusts liberated by the spreading of straw bedding in five French dairy farms located in Brittany...
July 20, 2017: Environmental Research
https://www.readbyqxmd.com/read/28735224/simultaneous-quantification-of-cortisol-and-cortisone-in-urines-from-infants-with-packed-fiber-solid-phase-extraction-coupled-to-hplc-ms-ms
#10
Chen Li, Zhao Zhang, Xiongwei Liu, Kangwei Shen, Pan Gu, Xuejun Kang
Cortisol and cortisone are two important glucocorticoids in human body, their interconversion is controlled by two isotypes of 11β-hydroxy steroid dehydrogenase (11β-HSD1 and 11β-HSD2). The ratio of urinary cortisol to cortisone can be used to assess the activity of 11β-HSDs. An analytical method to quantify urinary cortisol and cortisone using high performance liquid chromatographic tandem mass spectrometry following a packed-fiber solid-phase extraction (PFSPE) was developed. The proposed method was validated and applied to determine the urinary cortisol and cortisone concentrations in infants...
July 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28735223/isomeric-separation-and-quantitation-of-di-2-ethylhexyl-trimellitates-and-mono-2-ethylhexyl-trimellitates-in-blood-by-lc-ms-ms
#11
Christine Höllerer, Johannes Müller, Thomas Göen, Elisabeth Eckert
A new and fast HPLC-method for the simultaneous determination of tri-(2-ethylhexyl) trimellitate (TOTM or TEHTM), its diesters 2,4-di-(2-ethylhexyl) trimellitate (2,4-DEHTM), 1,4-di-(2-ethylhexyl) trimellitate (1,4-DEHTM), 1,2-di-(2-ethylhexyl) trimellitate (1,2-DEHTM) and monoesters 1-mono-(2-ethylhexyl) trimellitate (1-MEHTM), 2-mono-(2-ethylhexyl) trimellitate (2-MEHTM) and 4-mono-(2-ethylhexyl) trimellitate (4-MEHTM) together with di-(2-ethylhexyl) phthalate (DEHP) and its primary metabolite mono-(2-ethylhexyl) phthalate (MEHP) in blood was developed and validated...
July 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28735192/nozzle-jet-printed-flexible-field-effect-transistor-biosensor-for-high-performance-glucose-detection
#12
Kiesar Sideeq Bhat, Rafiq Ahmad, Jin-Young Yoo, Yoon-Bong Hahn
Printable electronics is a subject of great interest for low-cost, facile and environmentally-friendly large scale device production. But, it still remains challenging for printable biosensor development. Herein, we present the fabrication of nozzle-jet printed flexible field-effect transistor (FET) glucose biosensor. The silver source-drain electrodes and ZnO seed layers were printed on flexible substrate by nozzle-jet printer followed by ZnO nanorods (ZnO NRs) synthesis and glucose oxidase (GOx) immobilization...
July 14, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28735178/development-of-a-real-time-prediction-model-of-driver-behavior-at-intersections-using-kinematic-time-series-data
#13
Yaoyuan V Tan, Michael R Elliott, Carol A C Flannagan
As connected autonomous vehicles (CAVs) enter the fleet, there will be a long period when these vehicles will have to interact with human drivers. One of the challenges for CAVs is that human drivers do not communicate their decisions well. Fortunately, the kinematic behavior of a human-driven vehicle may be a good predictor of driver intent within a short time frame. We analyzed the kinematic time series data (e.g., speed) for a set of drivers making left turns at intersections to predict whether the driver would stop before executing the turn...
July 20, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28735163/multidimensional-human-capital-formation-in-a-developing-country-health-cognition-and-locus-of-control-in-the-philippines
#14
Kira M Villa
Economic success depends on multiple human capital stocks whose production is interrelated and occurs over many life stages. Yet, much empirical work fails to account for human capital's multidimensional nature and limits its focus to specific childhood stages. Using longitudinal data from the Philippines, I estimate a model of multidimensional human capital formation from birth through adulthood where health, cognitive, and noncognitive dimensions are jointly produced. I examine during which developmental stages parental investment is most influential and address the endogeneity of investment using a policy function where investment depends on child characteristics, exogenous conditions at birth and local prices...
July 8, 2017: Economics and Human Biology
https://www.readbyqxmd.com/read/28735076/quantifying-the-influence-of-oscillatory-flow-disturbances-on-blood-flow
#15
Sargon A Gabriel, Yan Ding, Yuqing Feng
Pulsatile blood flow is renowned for inducing localised flow disturbances that are characterised by significant oscillations. These flow disturbances are recognised to have a physiologic significance within the cardiovascular system, particularly with respect to their proatherogenic expression on endothelial cells. Flow disturbances also impart significant influence on the mechanics of cardiovascular flow and are formally shown in the present study to be coupled to the period-average behaviour of a pulsatile flow field, causing it to be misrepresented by its steady-equivalent; which is often used in models of atherogenesis and hence limits their reliability...
July 19, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28735070/suppression-of-the-atp-binding-cassette-transporter-abcc4-impairs-neuroblastoma-tumour-growth-and-sensitises-to-irinotecan-in%C3%A2-vivo
#16
Jayne Murray, Emanuele Valli, Denise M T Yu, Alan M Truong, Andrew J Gifford, Georgina L Eden, Laura D Gamble, Kimberley M Hanssen, Claudia L Flemming, Alvin Tan, Amanda Tivnan, Sophie Allan, Federica Saletta, Leanna Cheung, Michelle Ruhle, John D Schuetz, Michelle J Henderson, Jennifer A Byrne, Murray D Norris, Michelle Haber, Jamie I Fletcher
The ATP-binding cassette transporter ABCC4 (multidrug resistance protein 4, MRP4) mRNA level is a strong predictor of poor clinical outcome in neuroblastoma which may relate to its export of endogenous signalling molecules and chemotherapeutic agents. We sought to determine whether ABCC4 contributes to development, growth and drug response in neuroblastoma in vivo. In neuroblastoma patients, high ABCC4 protein levels were associated with reduced overall survival. Inducible knockdown of ABCC4 strongly inhibited the growth of human neuroblastoma cells in vitro and impaired the growth of neuroblastoma xenografts...
July 20, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28735045/multiplexed-capillary-microfluidic-immunoassay-with-smartphone-data-acquisition-for-parallel-mycotoxin-detection
#17
Jessica M D Machado, Ruben R G Soares, Virginia Chu, João P Conde
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure...
July 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28735041/self-assembled-polypeptide-nanoparticles-for-intracellular-irinotecan-delivery
#18
N N Zashikhina, M V Volokitina, V A Korzhikov-Vlakh, I I Tarasenko, A Lavrentieva, T Scheper, E Rhuel, R V Orlova, T B Tennikova, E G Korzhikova-Vlakh
In this research poly(l-lysine)-b-poly(l-leucine) (PLys-b-PLeu) polymersomes were developed. It was shown that the size of nanoparticles depended on pH of self-assembly process and varied from 180 to 650nm. The biodegradation of PLys-b-PLeu nanoparticles was evaluated using in vitro polypeptide hydrolysis in two model enzymatic systems, as well as in human blood plasma. The experiments of the visualization of cellular uptake of rhodamine 6g-loaded and fluorescein-labeled nanoparticles were carried out, which approved the possibility of their penetration into the cells...
July 19, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28735021/wisp1-ccn4-aggravates-cartilage-degeneration-in-experimental-osteoarthritis
#19
Martijn H van den Bosch, Arjen B Blom, Vardit Kram, Azusa Maeda, Sheena Sikka, Yankel Gabet, Tina M Kilts, Wim B van den Berg, Peter L van Lent, Peter M van der Kraan, Marian F Young
OBJECTIVE: Increased Wisp1 expression was previously reported in experimental and human osteoarthritis. Moreover, adenoviral overexpression of Wisp1 in naïve mouse knee joints resulted in early OA-like cartilage lesions. Here, we determined how the matricellular protein WISP1 is involved in the pathology that occurs in the complex osteoarthritic environment with aging and experimental osteoarthritis in wild type and Wisp1(-/-) mice. METHODS: Wild type and Wisp1(-/-) mice were aged or experimental osteoarthritis was induced with intraarticular collagenase injection, destabilization of the medial meniscus or anterior cruciate ligament transection...
July 19, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28735018/safety-and-feasibility-of-direct-mri-guided-transperineal-prostate-biopsy-using-a-novel-mri-safe-robotic-device
#20
Mark W Ball, Ashley E Ross, Kamyar Ghabili, Chunwoo Kim, Changhan Jun, Doru Petrisor, Li Pan, Jonathan I Epstein, Katarzyna J Macura, Dan S Stoianovici, Mohamad E Allaf
OBJECTIVE: To evaluate safety and feasibility in a first in human trial of direct MRI-guided prostate biopsy using a novel robotic device. METHODS: MrBot is an MRI-safe robotic device constructed entirely of nonconductive, nonmetallic, and nonmagnetic materials, developed by our group. A safety and feasibility clinical trial was designed to assess the safety and feasibility of direct MRI-guided biopsy with MrBot and determine its targeting accuracy. Men with elevated PSA, prior negative prostate biopsy and cancer suspicious region (CSR) on MRI were enrolled in the study...
July 19, 2017: Urology
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